KR20210131527A - Multifunctional non-alcoholic sanitizer and its manufacturing method - Google Patents

Abstract

The present invention relates to a multifunctional alcohol-free disinfectant composition and a method for preparing the composition, wherein 10 to 20 parts by weight of a propolis extract, 8 to 9 parts by weight of a niosome containing a clove extract, and 3 to 5 parts by weight of a niosome containing a pogongyeong extract parts by weight, 5 to 7 parts by weight of glycerin, 2.5 to 3.5 parts by weight of 1.2-hexanediol, 10 to 20 parts by weight of sodium salicylate, 4.5 to 5.5 parts by weight of disodium EDTA, and 0.5 parts by weight of methylpropanediol - 1 part by weight, 1.5 to 2.5 parts by weight of menthol, 2.5 to 3.5 parts by weight of butylene glycol, 3 to 5 parts by weight of polysorbate-20, and a multifunctional alcohol-free disinfectant composition comprising the remaining amount of electrolyzed water Accordingly, it is characterized in that it is hypoallergenic compared to conventional alcohol-based disinfectants and provides high skin affinity and penetration of active ingredients to prepare a disinfectant composition with excellent sterilization and durability.

Description

Multifunctional alcohol-free disinfectant composition and composition manufacturing method {MULTIFUNCTIONAL NON-ALCOHOLIC SANITIZER AND ITS MANUFACTURING METHOD}

The present invention relates to a multifunctional non-alcoholic disinfectant composition and a method for manufacturing the composition, and more particularly, it has excellent sterilization power and is distinguished from existing alcohol-based disinfectants to prepare a non-alcoholic disinfectant composition containing a natural substance as a main component. It is about the provision of a dramatic disinfectant.

In general, a disinfectant is a drug prepared to promote sterilization and cleaning effect by applying or rubbing instead of washing with water in order to prevent various infectious diseases.

Disinfectants were mainly used in medical institutions for the purpose of preventing the intrusion of microorganisms surgically into patients who are vulnerable to infection. Interest in detergents and the like is increasing.

Disinfectants commonly used are provided in liquid or gel-type products for easy use by keeping them in tubes or pumps, and consist of about 60 to 80% alcohol and other various chemicals to exert a sterilizing effect.

Alcohol, the main component of the disinfectant, works to remove various viruses, bacteria, bacteria, molds, etc. by applying ethanol or isopropane.

As an example of a known technique, Korean Patent Registration No. 10-1284045 discloses 0.01 to 2.0% by weight of a maleic anhydride-based polymer, 0.01 to 5.0% by weight of a cellulosic water-soluble polysaccharide, and at least one selected from methanol, ethanol and isopropanol. 40 to 95% by weight of a lower alcohol consisting of a species, 0.001 to 10% by weight of a polymer containing 2-(meth)acryloyloxyethylphosphorylcholine, water, pH and viscosity modifiers, disinfectants, humectants, and these It constitutes a gel-like finger disinfectant further comprising one or two or more additives selected from the group consisting of a mixture of

As another example, Korean Patent Registration No. 10 - 1583850 discloses an emulsifier and an emollient, a silicone, and a skin protectant selected from the group consisting of mixtures of 0.1 to 10% by weight of a high internal phase emulsion, methanol, ethanol, propanol, iso 20 to 95% by weight of an alcohol selected from the group consisting of propyl alcohol, butanol, t-butanol, 2-butanol, pentanol, hexanol, and mixtures thereof, with water, a wetting agent, a disinfectant, a viscosity increasing agent, a perfume; A moisturizing hand sanitizer further comprising an ingredient selected from the group consisting of pH adjusting agents, skin conditioning agents, and mixtures thereof.

As another example, Korean Patent Registration No. 10 - 1196113 discloses a step of measuring anhydrous ethanol and mixing it with essential oil to prepare anhydrous ethanol and a first mixed water of essential oil, and an herbal extract, aloe vera gel, Contains a natural herbal extract comprising the steps of preparing a second mixed water mixing hyaluronic acid, grapefruit seed extract, and purified water, and packaging and storing the second mixed water in a container and storing it at room temperature for 1 to 2 days constitute a method for manufacturing hand sanitizer that

Korean Patent Registration No. 10 - 1284045 (2013.07.09) Korean Patent No. 10 - 1583850 (2016.01.08) Korean Patent Registration No. 10 - 1196113 (2012.10.30) Korean Patent Registration No. 10 - 1653436 (2016.09.01)

The disinfectant to which the prior art as described above is applied contains 60 to 80% of alcohol such as ethanol or isopropane as a main component to achieve a sterilization action, and other forms containing chemicals such as skin moisturizers or emulsifiers for physical properties is made of

However, conventional disinfectants can cause various side effects such as erythema and rash when used by people with weak and sensitive skin due to the strong volatility and irritation of alcohol, and when used in the eyes, mouth, mucous membranes or areas with wounds, severe irritation is accompanied. There is a problem to do.

Due to this problem, as in the prior art as another example, a disinfectant manufacturing technique containing a natural ingredient known to have a bactericidal action, such as an herbal extract or aloe vera, is provided.

However, even if an emulsifier or a wetting agent is used in the process of mixing natural and chemical ingredients to form a disinfectant, the formulation of the active ingredient is unstable. It contains problems that are difficult to obtain.

Accordingly, the present invention was invented to solve the problems of the prior art as described above,

10 to 20 parts by weight of propolis extract, 8 to 9 parts by weight of niosome containing clove extract, 3 to 5 parts by weight of niosome containing pogongyeong extract, 5 to 7 parts by weight of glycerin, and 1.2-hexanediol 2.5 to 3.5 parts by weight, 10 to 20 parts by weight of sodium salicylate, 4.5 to 5.5 parts by weight of disodium EDTA, 0.5 to 1 part by weight of methylpropanediol, 1.5 to 2.5 parts by weight of menthol, and butylene; By containing 2.5 to 3.5 parts by weight of Lycol, 3 to 5 parts by weight of Polysorbate-20, and the remaining amount of electrolyzed water, it is hypoallergenic and provides high skin affinity and penetration of active ingredients, and is non-alcoholic with excellent sterilization and durability. It is possible to achieve the purpose of preparing a disinfectant composition.

The present invention is to prepare a non-alcoholic disinfectant composition containing propolis, cloves, and pogongyeong, which are natural ingredients known to have sterilization, antibacterial, detoxification, and the like, as active ingredients.

Therefore, the present invention produces a disinfectant composition that is hypoallergenic and has excellent sterilization power compared to a conventional disinfectant containing about 60 to 80% alcohol, so that it is effective to provide a disinfectant that can be safely used even by people with sensitive skin.

In addition, the present invention is a disinfectant of a formulation having high elasticity and dispersion stability by forming a nano-sized active ingredient delivery system by applying niosome technology, which is a double-membrane transporter using a non-ionic surfactant to derive an effective action of the active ingredient. There are advantages to preparing the composition.

Therefore, the present invention provides a high skin affinity and penetrability compared to the prior art, and secures the ability to encapsulate polar and non-polar components, so that the feeling of use and sterilization is excellent, and by preventing oxidation and denaturation of active ingredients, a disinfectant composition with improved durability It has a manufacturing effect.

1 is an image of an antimicrobial activity test result of cloves contained in a multifunctional alcohol-free disinfectant composition according to the present invention.
Figure 2 is an image of the particle size analysis test result of niosomes containing clove extract and pogongyeong extract contained in the multifunctional alcohol-free disinfectant composition according to the present invention.
3 to 5 are images of a test report for measuring the sterilization power of the multifunctional alcohol-free disinfectant composition according to the present invention.

Hereinafter, the composition and action according to a preferred embodiment of the multifunctional non-alcoholic disinfectant composition and method for preparing the composition of the present invention will be described in detail with reference to the accompanying drawings. In the following description, a detailed description of a part that can be easily implemented by a person skilled in the art may be omitted. In addition, since the following description is given by way of a preferred embodiment of the present invention, the present invention is not limited by the following examples, and it is natural that various modifications may be provided within the scope of the present invention. will be.

1 is an image of an antibacterial activity test result of cloves contained in a multifunctional non-alcoholic disinfectant composition according to the present invention, FIG. 2 is a particle size analysis of niosomes containing a clove extract and pogongyeong extract contained in a multifunctional alcohol-free disinfectant composition according to the present invention The test result image, FIGS. 3 to 5 shows an image of a test report for measuring the sterilization power of the multifunctional alcohol-free disinfectant composition according to the present invention.

The multifunctional non-alcoholic disinfectant composition to which the technology of the present invention is applied and the method for producing the composition are differentiated from conventional alcohol-based disinfectants to prepare a non-alcoholic disinfectant composition containing a natural substance as an active ingredient, thereby providing an excellent sterilizing power and hypoallergenic disinfectant Note that it is about

For this purpose, the disinfectant composition of the present invention contains 10 to 20 parts by weight of propolis extract, 8 to 9 parts by weight of niosome containing clove extract, 3 to 5 parts by weight of niosome containing pogongyeong extract, and 5 to 7 parts by weight of glycerin. Parts by weight, 2.5 to 3.5 parts by weight of 1.2-hexanediol, 10 to 20 parts by weight of sodium salicylate, 4.5 to 5.5 parts by weight of disodium EDTA, 0.5 to 1 part by weight of methylpropanediol, and 1.5 parts by weight of menthol ~ 2.5 parts by weight, 2.5 to 3.5 parts by weight of butylene glycol, 3 to 5 parts by weight of polysorbate-20, and the remaining amount of electrolyzed water.

The propolis is a substance made by mixing secretions such as sap, flower pollen, bee saliva, and wax collected from plants such as pine trees, fir trees, and willows during the process of bees building a honeycomb. Since propolis contains ingredients such as minerals, vitamins, amino acids, anisotropics, organic acids, flavonoids, and terpenes, it has been used as a natural antibiotic against bacteria, viruses, and bacteria since ancient times.

The propolis extract is obtained by extracting propolis collected from a honeycomb with a solvent, filtration, purification, and concentration of beeswax. The solvent used for propolis extraction may be water or an organic solvent.

The propolis extract is composed of 10 to 20 parts by weight based on the total weight of the disinfectant composition to prevent infection and treatment, and to prevent odor and decay as well as anti-inflammatory action.

The clove is a bud of a clove tree and includes reddish-brown burns, sepals, petals, stamens, and pistils. Cloves are rich in antioxidants such as eugenol, beta-caryophyllene, vanillin, and tannin, as well as calcium and vitamins, and are used as anti-inflammatory, sterilizing and anti-inflammatory agents.

The clove extract is obtained by extracting dried cloves with a solvent, purifying the solvent, and treating with a hydrolase. The solvent used for extracting cloves may be water or an organic solvent.

On the other hand, the disinfectant composition according to the present invention is configured to contain the clove extract by processing in the form of niosomes.

The niosomes containing the clove extract are obtained by emulsifying a nonionic surfactant and cholesterol to obtain a niosome having a micro-sized double membrane structure, and capturing the clove extract inside the niosome and stabilizing it in a nanoparticle formulation.

The niosomes containing the clove extract are nonionic surfactant-based vesicles with a hydrophilic end facing outward and a hydrophobic end facing each other to form a double membrane. Niosomes are similar to liposomes in that they have a double membrane structure, but niosomes have excellent stability by capturing both hydrophilic and lipophilic active ingredients in an aqueous or lipid membrane. The niosome containing the clove extract is configured to exhibit high delivery and penetration of the active ingredient by controlling the particle size to a nano level and having an average particle size of 50 to 500 nm.

The niosome containing the clove extract is configured to kill and inhibit bacteria, bacteria, etc. by adding 8 to 9 parts by weight based on the total weight of the disinfectant composition, so as to have an antiseptic action and an anti-aging action on the skin.

The pogongyeong is a dried dandelion outpost of the Asteraceae family and contains flavonoids, luteolin-glucoside, carotene, ascorbic acid, vitamins, and the like, and was used as an antibacterial, anti-inflammatory, antibacterial, and anti-allergic agent.

The pogongyeong extract is obtained by extracting the dried pogongyeong with a solvent, purifying the solvent, and then concentrating. Water or an organic solvent may be applied as the solvent used for the pore extraction.

The disinfectant composition according to the present invention is configured to contain the pogongyeong extract by processing it in the form of niosomes.

The niosome containing the pogongyeong extract is obtained by emulsifying a nonionic surfactant and cholesterol to obtain a niosome having a micro-sized double membrane structure, and capturing the pogongyeong extract inside the niosome and stabilizing it in a nanoparticle formulation.

The niosomes containing the pogongyeong extract are nonionic surfactant-based vesicles with a hydrophilic end facing outward and a hydrophobic end facing each other to form a double membrane. Niosomes are similar to liposomes in that they have a double membrane structure, but niosomes have excellent stability by capturing both hydrophilic and lipophilic active ingredients in an aqueous or lipid membrane. The niosome containing the pogongyeong extract is configured to exhibit high delivery and penetration of active ingredients by controlling the particle size to a nano level and having an average particle size of 50 to 500 nm.

The niosome containing the pogongyeong extract is configured to have antioxidant and detoxifying action by adding 3 to 5 parts by weight based on the total weight of the disinfectant composition.

The glycerin is provided to contain 5 to 7 parts by weight based on the total weight of the disinfectant composition to supply moisture to the skin and prevent drying of the disinfectant itself.

The 1.2-hexanediol (1.2-Hexandiol) is prepared to contain 2.5 to 3.5 parts by weight based on the total weight of the disinfectant composition so as to act as a colorless and odorless natural moisturizing, antibacterial and antifungal agent.

The sodium salicylate (Sodium Salicylate) is provided so as to contain 10 to 20 parts by weight based on the total weight of the disinfectant composition to act as a denaturant and sterilizing preservative.

The disodium EDTA is provided so as to contain 4.5 to 5.5 parts by weight based on the total weight of the disinfectant composition in order to secure safety by inhibiting bacterial infection, antioxidant, and the generation of deposits.

The methylpropanediol is prepared to contain 0.5 to 1 part by weight based on the total weight of the disinfectant composition to act as a colorless and odorless organic solvent, solvent, and moisturizer.

The menthol is provided so as to contain 1.5 to 2.5 parts by weight based on the total weight of the disinfectant composition so as to give a refreshing feeling and a refreshing feeling and to provide a moisturizing effect.

The butylene glycol is provided so as to contain 2.5 to 3.5 parts by weight based on the total weight of the disinfectant composition to act as a solvent, a moisturizer, a viscosity reducing agent, and a skin conditioning agent.

The polysorbate-20 (Polysorbate 20) acts to achieve emulsion stability by uniformly dispersing ingredients that do not mix well with each other, such as natural and chemical substances constituting the disinfectant composition of the present invention as described above, and the disinfectant composition It is provided so as to contain 3 to 5 parts by weight based on the total weight.

The electrolyzed water is provided to contain an appropriate amount to sterilize bacteria or bacteria and act as a preservative by generating an oxidizer in a short time by inducing an electrolysis reaction by applying negative and positive currents to water.

In addition, the disinfectant composition of the present invention is provided to contain sodium hypochlorite (HCLO) to promote disinfection and deodorization, decay, and killing of pathogens.

Hereinafter, a method for producing a multifunctional alcohol-free disinfectant composition to which the technology of the present invention is applied having the configuration as described above will be described.

The step of composing the first mixture is a step of stirring sodium salicylate, glycerin, and 1.2-hexanediol at room temperature for 10 to 20 minutes.

The step of composing the first mixture is carried out in the aqueous phase, and is configured to impart sterilization and preservative power using electrolyzed water, and 10 to 20 parts by weight of sodium salicylate based on the total weight of the resulting disinfectant composition; 5 to 7 parts by weight of glycerin and 2.5 to 3.5 parts by weight of 1.2-hexanediol are mixed and stirred at low speed to form a first mixture.

The step of obtaining the second mixture is a step of stirring propolis, butylene glycol, and polysorbate-20 at 45° C. for 5 minutes.

In the step of obtaining the second mixture, 10 to 20 parts by weight of propolis extract, 2.5 to 3.5 parts by weight of butylene glycol, and 3 to 5 parts by weight of polysorbate-20 are blended as a natural active ingredient and using a homomixer A second mixture is prepared by stirring at 1500 rpm.

The step of composing the first composition is a step of forming the first composition by adding the second mixture to the first mixture prepared as described above.

In the step of obtaining the secondary composition, a niosome containing a clove extract, a niosome containing a pogongyeong extract, and a disodium edti A, menthol, sodium hypochlorite, and methylpropanediol are added and mixed at room temperature for 10 minutes to obtain a secondary composition.

In the step of obtaining the secondary composition, 8 to 9 parts by weight of niosome containing clove extract as a natural active ingredient, 3 to 5 parts by weight of niosome containing pogongyeong extract, and 4.5 to 5.5 weight of disodium EDTA Part and 1.5 to 2.5 parts by weight of menthol, sodium hypochlorite and methylpropanediol are mixed in an appropriate amount and mixed at 800 rpm using a homomixer to obtain a secondary composition.

Niosomes containing clove extract and niosomes containing pogongyeong extract, which are added to the step of obtaining the secondary composition, are emulsified with a nonionic surfactant and cholesterol to obtain niosomes having a fine-sized double membrane structure, and cloves The extract and the pogongyeong extract are captured inside each niosome and obtained through a process of stabilizing the nanoparticle formulation. As for the formation mechanism of niosomes, reference will be made to the well-known related art, and thus a detailed description thereof will be omitted.

Injecting clove and porgongyeong extract containing the active ingredient of the multifunctional alcohol-free disinfectant composition according to the present invention into the formulation of niosomes ensures stability by capturing both hydrophilic and lipophilic active ingredients inside the double membrane and niosomes. This is in order to exert high delivery and penetration of active ingredients by controlling the size of the particles at the nano level.

The secondary composition obtained as described above is cooled to room temperature and filtered through a 180-220 mesh filter, and after storing and aging the filtered secondary composition to obtain a disinfectant composition, a multifunctional alcohol-free disinfectant composition as a final product is obtained. manufacture

Hereinafter, embodiments and test examples including the present invention having the configuration as described above will be constructed, and the effects thereof will be understood in detail.

<Example>

A multifunctional alcohol-free disinfectant composition is prepared by performing the steps of preparing the first mixture according to the present invention as described above to obtaining the disinfectant composition. Table 1 below shows the mixing ratio of each component with respect to the total weight of the disinfectant composition.

Ingredient name propolis extract glycerin 1.2-hexanediol Sodium Salicylate HCLO Disodium EDTA methyl propanediol Niosomes Containing Clove Extract Niosomes Containing Pogongyoung Extract menthol Butylene Glycol Polysorbate 20 electrolyzed water Mixing ratio (wt%) 10-20 5-7 3 10-20 a very small amount 5 0.5~1 8~9 3-5 2 3 3-5 remaining amount

<Test Example 1>

The antibacterial activity test of cloves contained as an active ingredient of the multifunctional alcohol-free disinfectant composition prepared according to the above example is carried out.

As the strains used for the antibacterial test, as shown in Table 2 below, the gram-positive bacteria Listeria and the gram-negative bacteria E. coli (Excheri chia coli) were used.

Type Bacterial strain KCTC No. Temperature(℃) Medium Gram-positive Listeria gray KCTC 3433 37 Bin heart infusio Gram-negative Excherichia coli KCTC 10115 38 Luria-bertari broth

A disk diffusion method was performed to measure the antibacterial activity. One day before the disc diffusion method, each strain was cultured in 5 mL of Luria-bertari broth (LB) at 200 rpm for 24 hours, and the number of bacteria ^{was used at 1×10 6} cfu/mL. The extract was treated so that the maximum concentration was 5 or 10 mg in a Petri dish spread evenly on a solid medium, and after complete absorption, incubated in an incubator at 37° C. for 24 hours to measure the diameter of the inhibition zone (nm) around the sample treatment. 1 shows an image of a culture result in E. coli (a) and Listeria (b). If the zone is present, there is activity, and the larger the zone, the stronger the activity. Therefore, it can be confirmed that the antibacterial activity of cloves is actively performed through the culture result image of FIG. 1 .

<Test Example 2>

A particle size analysis test of niosomes containing clove extract and pogongyeong extract contained as an active ingredient of the multifunctional non-alcoholic disinfectant composition prepared by the above example is conducted. 2 is a graph showing a particle size distribution graph and average particle size measurement results of niosomes. When the niosome particles are composed at the nano level, the delivery power and penetration power of the active ingredient are significantly increased, and the average particle size of the niosome particles containing the clove extract and pogongyeong extract according to the present invention is 86.0 nm through the results of FIG. 2 . It can be confirmed that

<Test Example 3>

A test for measuring the sterilization power of the multifunctional alcohol-free disinfectant composition prepared according to the above example is carried out.

Inoculation strains used for the sterilization test were Pseudomonas aeruginosa, Salmonella Typhimurium, and Bacillus subtilis.

The test solution is 1 mL (g) of the multifunctional non-alcoholic disinfectant composition sample according to the present invention: 0.1 mL of the test bacteria solution, and the control test solution is PBS (Phosphate Buffer Saline) 1 mL: 0.1 mL of the test bacteria solution.

After mixing the sample and each test microbial solution, diffusion culture is performed after 5 minutes. The sample collection amount is 1mL, and in order to measure the number of viable cells, 10 fold serial dilution, 0.1 ml of each dilution, and diffusion culture are repeated twice. After culturing at 37°C for 22±2h using TSA medium, the number of bacteria is counted. to test The control test solution is also tested in the same way as the sample. Through the test results of FIGS. 3 to 5 and Table 3, it can be confirmed that the sterilizing power of the multifunctional alcohol-free disinfectant composition according to the present invention exhibits excellent results.

exam
strain contact
hour Number of bacteria (CFU/mL) Sterilization (%) Control test solution (A) Test solution (B) Pseudomonas aeruginosa 5 minutes 8.6×10 ⁵ <10 99.99 Salmonella 5 minutes 1.5×10 ⁶ <10 99.99 Bacillus subtilis 5 minutes 4.8×10 ⁵ <10 99.99

The multifunctional non-alcoholic disinfectant composition and composition manufacturing method according to the present invention as described above is configured to prepare an alcohol-free disinfectant composition containing propolis, cloves, and pogongyeong as active ingredients, so that about 60 to 80% of the conventional disinfectant consists of an alcohol component. There is an advantage in that it is possible to provide a disinfectant composition that is hypoallergenic and has excellent sterilization power.

In addition, the present invention is a disinfectant of a formulation having high elasticity and dispersion stability by forming a nano-sized active ingredient delivery system by applying niosome technology, which is a double-membrane transporter using a non-ionic surfactant to derive an effective action of the active ingredient. There are advantages to preparing the composition.

Therefore, the present invention provides a high-quality, multi-functional alcohol-free disinfectant composition with improved durability by preventing oxidation and denaturation of active ingredients, which provides high skin affinity and penetration, has excellent feeling and sterilization power, compared to disinfectants according to the prior art. there is

Not applicable.

Claims (2)

10 to 20 parts by weight of propolis extract, 8 to 9 parts by weight of niosome containing clove extract, 3 to 5 parts by weight of niosome containing pogongyeong extract, 5 to 7 parts by weight of glycerin, and 1.2-hexanediol 2.5 to 3.5 parts by weight, 10 to 20 parts by weight of sodium salicylate, 4.5 to 5.5 parts by weight of disodium EDTA, 0.5 to 1 part by weight of methylpropanediol, 1.5 to 2.5 parts by weight of menthol, and butylene; Lycol 2.5 to 3.5 parts by weight, polysorbate-20 3 to 5 parts by weight, and the remaining amount of electrolyzed water,
The multifunctional alcohol-free disinfectant composition, characterized in that the average particle size of the niosome containing the clove extract and the niosome particle containing the pogongyeong extract is 50 ~ 500nm. A first mixture is prepared by stirring sodium salicylate, glycerin, and 1.2-hexanediol at room temperature for 10 to 20 minutes;
Stirring propolis, butylene glycol, and polysorbate-20 at 45° C. for 5 minutes to obtain a second mixture;
adding a second mixture to the first mixture to form a primary composition;
Niosome containing clove extract, niosome containing clove extract, disodium EDTA, menthol, sodium hypochlorite, and methylpropanediol were added to the primary composition and mixed at room temperature for 10 minutes. obtaining a secondary composition;
Cooling the secondary composition to room temperature and filtering with a 180-220 mesh filter;
A method for producing a multifunctional alcohol-free disinfectant composition comprising the step of obtaining a disinfectant composition by storing and aging the filtered secondary composition.

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