KR20200072608A - Cosmetic Composition For Skin Whitening Comprising Extract of Clitoria Ternatea Flower - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening and antioxidant containing a Clitoria Ternatea flower extract as an active component. The cosmetic composition of the present invention has an effect of inhibiting tyrosinase activity and an effect of inhibiting melanin production, and also has excellent stability by not irritating the skin.

Description

Cosmetic composition for skin whitening including butterfly bean flower extract {Cosmetic Composition For Skin Whitening　Comprising Extract of Clitoria Ternatea Flower}

The present invention relates to a cosmetic composition for skin whitening containing a butterfly bean extract as an active ingredient. The cosmetic composition of the present invention has an inhibitory effect on tyrosinase activity and an inhibitory effect on melanin production, and is excellent in stability because it does not irritate the skin.

The goal of cosmetics is to maintain beautiful and healthy skin, as well as to delay the aging of naturally occurring skin with age. In general, aging is due to oxidative damage of biomaterials by free radicals. In other words, highly reactive free radicals such as free radicals, such as hydrogen peroxide, hydroxy radicals, and superoxide radicals, peroxidize the lipids that make up the cell membrane, destroying the cell membrane, oxidizing proteins, attacking nucleic acids, and causing oxidative damage to biomaterials Destroy the cells and eventually kill the cells.

The most significant skin characteristics due to aging include wrinkles, reduced elasticity, and dull skin. In addition, in addition to wrinkles and decreased elasticity, after the 40s, the face color is often unclear and gray and dark. Particularly, in the case of young women, as a result of childbirth, blood bleeding occurs and blood circulation disorders occur, resulting in a decrease in metabolic ability and skin roughening and dullness. In addition, it is everyone's desire to have white and fine skin. In general, the color of human skin, hair and eyes is determined by melanin, carotene and hemoglobin.

In particular, melanin is the most important factor for determining skin color, and skin color is determined according to the amount, nature, and distribution of melanin. The pigment of human skin or hair serves to protect the skin or hair from the harmful effects of sunlight, especially ultraviolet rays. For example, a person who lacks such a pigment is very sensitive to sunlight and is prone to burns, and even at a young age, the likelihood of skin cancer is high. Short-wavelength ultraviolet rays (290-320 nm) and carcinogens form harmful radicals, such as oxygen radicals, in the skin, and these oxygen radicals are known to attack skin cells and age the skin. The main function of melanin is to remove these harmful radicals and protect the skin from damage. Therefore, the high amount of melanin means that it has an effective response system to protect the skin from physical or chemical toxic substances. Factors that promote the production of melanin include sunlight (ultraviolet rays), as well as estrogen or prostaglandin. On the other hand, melanin is produced through complex oxidation and condensation reaction after the tyrosine is converted to dopa (DOPA) and dopachrome by an enzyme called tyrosinase in melanocytes by ultraviolet rays. At this time, the produced melanin is transmitted to the skin cells and exhibits a circulating action in which melanin is lost and disappears with epidermal detachment. This process of melanin production is a natural phenomenon, and does not occur excessively of melanin in normal human skin. However, when the skin reacts to external stimuli such as ultraviolet rays, environmental pollution or stress, melanin is excessively produced. When the over-produced melanin is not discharged from the skin and remains in the skin, pigmentation occurs, resulting in spots and freckles.

Among the external stimulation factors, ultraviolet rays are the largest source of melanin biosynthesis and may affect various processes related to melanin production. That is, ultraviolet rays act as important factors for inducing melanin overproduction by promoting the activity of melanocytes, melanocytes, promoting melanin biosynthesis stimulating hormone secretion, promoting melanin oxidation, or promoting tyrosinase activity. The biggest feature of this melanin-producing mechanism is that only one enzyme, tyrosinase, is involved, and the whitening effect can be expected when the tyrosinase activity is inhibited to prevent melanin production. Among the prior art, it is known to use ascorbic acid as a whitening material (Japanese Patent Publication No. Hei 4-9320). However, ascorbic acid has poor phase stability in the formulation, and there is a problem in that the activity of the active ingredient decreases over time. Therefore, in recent years, various methods such as stabilizing ascorbic acid with capsules or liposomes have been proposed, but a clear stabilization method has not yet been proposed.

It is known to use hydroquinone as another whitening material (JP-A-6-192062). Hydroquinone has an excellent effect, but because it is a carcinogenic substance, it is limited in its use as a cosmetic. It is known to use kojic acid as another whitening material (Japanese Patent Publication No. Hei 56-7710). Kojic acid exhibits excellent whitening effect due to its excellent inhibitory ability of tyrosinase, but it also has a problem in terms of safety in the formulation and was recently released as a carcinogen.

It is known to use arbutin as another whitening material (Japanese Patent Publication No. Hei 4-9315). Arbutin can be obtained from alpine bilberries or obtained synthetically, and its inhibitory ability against tyrosinase, like kojic acid, has been demonstrated. However, arbutin itself is a state in which sugar is attached to hydroquinone, and when applied to cosmetic products, sugar is separated by skin enzymes, causing skin irritation caused by hydroquinone.

As a result, the present inventors tried to overcome the problems of the conventional skin whitening cosmetic composition, i.e., stability deterioration, skin side effects and weakening of the whitening effect. As a result, the butterfly bean flower extract has excellent tyrosinase activity inhibitory effect and , Dopachrome exhibits an automatic antioxidant inhibitory effect and an inhibitory effect on melanocyte production of melanocytes, and has completed the present invention by confirming that it has excellent antioxidant power and no skin irritation.

An object of the present invention is to provide a cosmetic composition excellent in skin whitening and antioxidant effects.

The above-described object is achieved by a cosmetic composition for skin whitening comprising a butterfly bean extract as an active ingredient.

Preferably, the butterfly flower extract is extracted using a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol. May be

Also preferably, the butterfly bean flower extract may be included in an amount of 0.001 to 30% by weight based on the total weight of the composition of the cosmetic.

Also preferably, the butterfly bean flower extract is composed of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray It may be a formulation selected from the group.

The butterfly bean extract of the present invention has an improved inhibitory effect on tyrosinase activity and an excellent melanosite production of melanin compared to the conventional whitening component, and the cosmetic composition comprising the butterfly bean extract of the present invention has an antioxidant effect and excellent skin whitening It shows effect. In addition, since the butterfly bean extract of the present invention hardly irritates the skin, it can be effectively used as an active ingredient of a cosmetic composition.

All technical terms used in the present invention, unless defined otherwise, have the following definitions and conform to the meaning as commonly understood by those skilled in the art in the relevant field of the present invention. In addition, although a preferred method or sample is described herein, similar or equivalent ones are included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, for reference amount, level, value, number, frequency, percent, dimension, size, amount, weight or length It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, the terms “comprises” and “comprising”, unless the context requires otherwise, include the steps or components presented, or groups of steps or components, but any other steps or components, or It should be understood that it implies that a group of steps or components is not excluded.

The present invention provides a cosmetic composition for skin whitening comprising a flower extract of Clitoria Ternatea as an active ingredient. The butterfly bean flower extract containing the active ingredient in the cosmetic composition of the present invention is an extract extracted from the flower of the butterfly bean.

Clitoria Ternatea is a plant belonging to the family Lepidoptera , also called Butterfly Pea and Green Pea, and is a perennial herbaceous plant with elliptical leaves. It grows well on moist neutral soil and is mainly distributed in Southeast Asia. It is said that it is used in heart disease, cerebrovascular disorders, hyperlipidemia, etc. in traditional Thai medicine, or it is said that it prevents aging of cells in beauty, and is used as a herbal tea. It is said that the flowers, leaves, and roots of butterfly beans have been used for a long time as a medicinal agent for enhancing memory, antidepressants, antistress, neurostabilizers, and sedatives. The flowers of butterfly beans are dried and used as herbal teas or added to foods to be used as a pigment, and the search is blue. Butterflies' flowers are rich in antioxidants, anthocyanins.

As used in the present invention, the term “butterfly flower extract” means an extract obtained from a flower of a butterfly bean. Preferably, the butterfly bean extract in the present invention is (a) water, (b) anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol and butanol, etc.), (c) acetone, (d) ethyl Solvents selected from the group consisting of acetate, (e) chloroform, (f) butyl acetate and (g) 1,3-butylene glycol were obtained from the flowers of butterfly beans. The extraction solvent is preferably a lower water alcohol, more preferably 70 to 95% alcohol, and most preferably 70% alcohol. A suitable amount of the extraction solvent is 1 to 20 times the dry weight of the butterfly flower, more preferably 1 to 10 times.

According to a specific embodiment of the present invention, the extraction is performed as follows. First, the butterfly bean flower is washed with purified water, then dried and crushed into small pieces, and the extractant 1 to 10 times the dry weight is added thereto. Thereafter, a cooling condenser is installed to extract the active ingredient from being evaporated for 3 to 20 hours at 40 to 100°C, or to extract for 1 to 15 days at 4 to 40°C, and dried completely using a rotary vacuum evaporator. do. At this time, when the extraction solvent is 1,3-butylene glycol, it is difficult to dry using a rotary reduced pressure evaporator, so it is prepared by directly extracting under the above conditions and adjusting the drying loss to 1% (w/v).

On the other hand, it is apparent to those skilled in the art that the extract of the present invention can obtain a butterfly bean flower extract having substantially the same effect even when using other extraction solvents as well as the aforementioned extraction solvent. In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract that has been subjected to a conventional purification process. Obtained through various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity) The fraction is also included in the butterfly bean flower extract of the present invention. The butterfly bean flower extract of the present invention can be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

Butterfly bean flower extract of the present invention is characterized in that it is used as a skin whitening component. The butterfly bean flower extract inhibits the production of melanin from tyrosine by inhibiting the activity of tyrosinase, and has an effect of inhibiting melanin production in melanocytes. The butterfly bean flower extract in the composition of the present invention contains an effective amount of the butterfly bean flower extract that can inhibit tyrosinase activity or inhibit melanin production in melanocytes. The cosmetic composition of the present invention may include other skin whitening components in addition to the butterfly bean flower extract as an active ingredient. However, the cosmetic composition of the present invention shows a very good skin whitening effect even as an active ingredient composed only of a butterfly bean flower extract.

According to a preferred embodiment of the present invention, the content of the butterfly bean flower extract is 0.001 to 30% by weight relative to the total weight of the cosmetic composition, preferably 0.01 to 20% by weight, more preferably 0.1 to 10.0% by weight. At this time, when the content of the butterfly bean flower extract contained in the cosmetic composition is less than the minimum content, there is a problem that the improvement of the whitening effect through inhibition of tyrosinase activity and inhibition of melanin production of melanocytes is somewhat insufficient, and exceeds the maximum content In the case, the apparent whitening effect is no longer increased and is likely to cause irritation to the skin, and has a great influence on the stabilization of the formulation.

The components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the active ingredients as the active ingredients. Conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , May be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder. When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as carrier components Can be used. When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in the case of a spray, additionally chlorofluorohydrocarbon, propane /Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspension agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or trakant, etc. can be used. When the formulation of the present invention is a surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as a carrier component Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail through experimental examples. These experimental examples are only for explaining the present invention in more detail, it is obvious to those skilled in the art to which the present invention pertains that the scope of the present invention is not limited by them according to the gist of the present invention. something to do.

Manufacturing example One: Butterfly Bean Flower Preparation of extract I

After washing with purified water and adding 200 g of dried Butterfly Soybean Flower to 1.2 L of water, extract the active ingredient by heating at 70-90° C. for 5 hours in an extractor equipped with a cooling condenser, and then filter with a 300 mesh filter cloth, 7- at 5-10° C. After standing for 10 days and aged, the filter was filtered with Whatman 5 filter paper. The filtrate was dried at 65° C. using a rotary vacuum evaporator to obtain a dry weight of 18.1 g. The dried powder was dissolved in 50% 1,3-butylene glycol solution to 1% (w/v) and used in the present invention.

Manufacturing example 2: Butterfly Bean Flower Preparation of extract II

After washing with purified water and adding 200 g of dried butterfly flowers to 1.2 L of water, extract the active ingredient for 5 days at 15-35° C., filter with a 300 mesh filter cloth, filter again with Whatman 5 filter paper, and double with a rotary vacuum evaporator. Concentrated. To this, 0.6 L of 100% ethanol was added, left to stand at 5-10°C for 7-10 days, aged, and then filtered with Whatman 5 filter paper. The filtrate was dried at 65° C. using a rotary vacuum evaporator to obtain a dry weight of 18.5 g. The dried powder was dissolved in 50% 1,3-butylene glycol solution to 1% (w/v) and used in the present invention.

Manufacturing example 3 to 21: Butterfly Bean Flower Preparation of extract III

Washed with purified water, 200 g of dried butterfly flowers were placed in 1.2 L of the extraction solvent of Table 1 below, and the active ingredient was extracted for 5 days at 4-40° C., filtered through a 300 mesh filter cloth, and left to stand at 5-10° C. for 7-10 days to mature. After being filtered, it was filtered with Whatman 5 filter paper. The filtrate was dried at 65° C. using a rotary vacuum evaporator to obtain a dry weight as shown in Table 1 below. The dried powder of each sample was dissolved in 50% 1,3-butylene glycol solution to 1% (w/v) and used in the present invention.

Manufacturing example number Extraction solvent Dry weight of final extract (unit: g) 3 water 15.2 4 10% ethanol 18.7 5 20% ethanol 19.8 6 30% ethanol 16.6 7 40% ethanol 18.5 8 50% ethanol 19.7 9 60% ethanol 19.4 10 70% ethanol 19.8 11 80% ethanol 18.2 12 90% ethanol 19.0 13 100% ethanol 18.5 14 Methanol 18.3 15 n-propanol 17.6 16 Isopropanol 15.6 17 2-butanol 15.2 18 Acetone 15.3 19 chloroform 16.4 20 Ethyl acetate 15.4 21 Butyl acetate 16.5

Manufacturing example 22: Butterfly Bean Flower Preparation of extract IV

After washing with purified water and adding 200 g of dry butterfly bean leaf into 1.2 L of 1,3-butylene glycol, extract the active ingredient for 48 hours, filter with a 300 mesh filter cloth, and stand at 5-10°C for 7-10 days to mature. After being filtered, it was filtered with Whatman 5 filter paper. This extract was again prepared for loss on drying to prepare a final concentration of 1% (w/v). The dried powder was dissolved in 50% 1,3-butylene glycol solution to 1% (w/v) and used in the present invention.

Manufacturing example 23 to 32: Butterfly Bean Flower Preparation of extract V

After washing with purified water and adding 200 g of dried butterfly bean leaves to 1.2 L of the extraction solvent of Table 2, heating was performed at 70-90° C. in an extractor equipped with a cooling condenser for 5 hours to extract the active ingredient, followed by filtration through a 300 mesh filter cloth. , Aged at 5-10° C. for 7-10 days, and then filtered with Whatman 5 filter paper. The extract was dried at 65° C. using a rotary vacuum evaporator to obtain a dry weight as shown in Table 2 below. The dried powder of each sample was dissolved in 50% 1,3-butylene glycol solution to 1% (w/v) and used in the present invention.

Manufacturing example number Extraction solvent Dry weight of final extract (unit: g) 23 10% ethanol 16.1 24 20% ethanol 18.5 25 30% ethanol 18.3 26 40% ethanol 18.6 27 50% ethanol 16.3 28 60% ethanol 17.0 29 70% ethanol 18.4 30 80% ethanol 17.2 31 90% ethanol 16.3 32 100% ethanol 18.0

Experimental Example One: Butterfly Bean Flower Experiment to inhibit tyrosinase activity of extract

40 μl of 1.5 mmol/L tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used as a substrate, and 0.05% of 1% (w/v) butterfly bean extract prepared in Preparation Examples 1 to 32 It was diluted with sodium phosphate buffer solution (pH 6.8) to prepare a sample solution having a volume ratio of 1% (v/v) (final butterfly flower extract concentration is 0.01% (w/v)). After adding 240 μl of the sample solution to 40 μl of tyrosine, 20 μl of tyrosinase (Sigma, 1500 U/ml) was added to the mixed solution, reacted at 37° C. for 15 minutes, and then absorbed at 490 nm. Was measured, and a butterfly bean extract not added was used as a control. Tyrosinase activity inhibition (%) was calculated according to Equation 1 below, and the results are shown in Table 3.

[Equation 1]

Test substance Inhibition rate of tyrosinase activity (%) Test substance Inhibition rate of tyrosinase activity (%) Preparation Example 1 64.5 Preparation Example 17 65.7 Preparation Example 2 65.4 Preparation Example 18 64.2 Preparation Example 3 67.4 Preparation Example 19 65.1 Preparation Example 4 64.3 Preparation Example 20 67.0 Preparation Example 5 65.2 Preparation Example 21 68.7 Preparation Example 6 65.6 Preparation Example 22 69.9 Preparation Example 7 64.3 Preparation Example 23 69.0 Preparation Example 8 65.8 Preparation Example 24 65.5 Preparation Example 9 66.9 Preparation Example 25 66.3 Preparation Example 10 68.5 Preparation Example 26 65.8 Preparation Example 11 65.0 Preparation Example 27 64.2 Preparation Example 12 65.7 Preparation Example 28 62.3 Preparation Example 13 67.4 Preparation Example 29 61.5 Preparation Example 14 68.7 Preparation Example 30 60.5 Preparation Example 15 66.4 Preparation Example 31 60.7 Preparation Example 16 68.2 Preparation Example 32 61.4

As can be seen from Table 3, the butterfly bean flower extract showed excellent tyrosinase activity inhibitory effect. Therefore, it was found that the composition containing the butterfly bean flower extract of the present invention exhibits an excellent inhibitory effect on tyrosinase activity.

Experimental Example 2: Butterfly Bean Flower Melanin production inhibition experiment of extract

B-16 cells (murine melanoma cells, Korea Cell Line Bank) were inoculated to a 12-well plate to be 104 cells/well and cultured for one day. The 1% (w/v) butterfly bean flower extract prepared in Preparation Examples 1 to 32 was diluted with 0.05 M sodium phosphate buffer (pH 6.8) in the same manner as in Experimental Example 1 to prepare a sample solution of 1% (v/v). (The final butterfly bean extract concentration is 0.01% (w/v)). After adding 240 µl of the sample solution to each well and incubating for 3-4 days, the supernatant of the culture solution of each well was removed and only melanocytes were centrifuged. The centrifuged cells were lysed with 500 μl of a 1N NaOH solution dissolved in dimethyl sulfoxide (DMSO) and heated in a 90° C. water bath for 10 minutes. After centrifugation again, the absorbance of the supernatant was measured at 570 nm, and as a control, a butterfly bean extract without treatment was used. The inhibition rate of melanin production was calculated according to Equation 2 below, and the results are shown in Table 4.

[Equation 2]

Test substance Melanin production inhibition rate (%) Test substance Melanin production inhibition rate (%) Preparation Example 1 44.3 Preparation Example 17 43.5 Preparation Example 2 47.1 Preparation Example 18 46.4 Preparation Example 3 64.2 Preparation Example 19 48.2 Preparation Example 4 44.1 Preparation Example 20 46.1 Preparation Example 5 45.0 Preparation Example 21 45.1 Preparation Example 6 45.4 Preparation Example 22 47.5 Preparation Example 7 46.3 Preparation Example 23 45.3 Preparation Example 8 44.3 Preparation Example 24 44.5 Preparation Example 9 47.1 Preparation Example 25 44.1 Preparation Example 10 48.5 Preparation Example 26 44.5 Preparation Example 11 45.1 Preparation Example 27 47.5 Preparation Example 12 46.5 Preparation Example 28 48.4 Preparation Example 13 46.1 Preparation Example 29 45.6 Preparation Example 14 46.6 Preparation Example 30 45.3 Preparation Example 15 44.0 Preparation Example 31 45.2 Preparation Example 16 48.5 Preparation Example 32 41.3

As can be seen in Table 4, it was confirmed that the butterfly bean flower extract has an excellent effect on the inhibition of melanin production in melanoma cells. Further, no cytotoxicity was observed in each experiment.

Hereinafter, a formulation example of a cosmetic composition containing a butterfly bean extract, for example, is specifically presented, but the composition of the present invention is not limited to these formulations.

Formulation example 1: Flexible makeup

The formulation example of the softening longevity including the butterfly bean extract obtained by the method of Preparation Example 10 was prepared with the composition of Table 5 according to the conventional method (Example 1), and the softening without the butterfly bean extract as a comparison group was added. Longevity (Comparative Example 1) was also prepared.

ingredient Content (% by weight) Example 1 Comparative Example 1 Butterfly Bean Flower Extract (Production Example 10) 1.50 - glycerin 8.00 8.00 Butylene Glycol 8.00 8.00 Tocopheryl acetate 0.01 0.01 EDTA-2Na 0.02 0.02 ethanol 3.00 3.00 PG-40 Hydrogenated Castor Oil a very small amount a very small amount Cellulose gum 0.50 0.50 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

Formulation example 2: milk Lotion

The formulation example of the milk lotion containing the butterfly bean extract obtained by the method of Preparation Example 10 was prepared with the composition of Table 6 according to a conventional method (Example 2), and as a comparative group, the milk without the butterfly bean extract was added. Lotion (Comparative Example 2) was also prepared.

ingredient Content (% by weight) Example 2 Comparative Example 2 Butterfly Bean Flower Extract (Production Example 10) 4 - glycerin 5 5 Dipropylene glycol 4.5 4.5 Tocopheryl acetate 3 3 Polyglyceryl-3 methyl glucose distearate 1.5 1.5 Squalane 3.2 3.2 Macadamia Nut Oil 1.5 1.5 Sorbitan sesquioleate 0.5 0.5 Sodium carbomer 0.2 0.2 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

Formulation example 3: nutrition cream

The formulation example of the nutrient cream containing the butterfly bean flower extract obtained by the method of Preparation Example 10 was prepared with the composition of Table 7 according to a conventional method (Example 3), and the butterfly bean flower extract was not added as a comparative group. A nutrition cream (Comparative Example 3) was also prepared.

ingredient Content (% by weight) Example 3 Comparative Example 3 Butterfly Bean Flower Extract (Production Example 10) 6 - glycerin 5 5 Dipropylene glycol 4.5 4.5 Tocopheryl Acetate 3 3 Polyglyceryl-3 methyl glucose distearate 2.5 2.5 Squalane 5 5 Macadamia Nut Oil 3 3 Vaseline One One Wax 0.5 0.5 EDTA-2Na 0.02 0.02 Sorbitan sesquioleate One One Sodium carbomer 0.8 0.8 incense a very small amount a very small amount antiseptic a very small amount a very small amount Purified water Balance Balance system 100 100

Experimental Example 3: Cosmetic Whitening effect of the composition

A whitening effect experiment of a cosmetic composition containing the butterfly bean flower extract of the present invention was conducted on 30 Korean women, especially 19 to 40 years old, with dark skin. The measurement sites are marked by 5 cm of the arm by 1 cm in width and width, and the cosmetic preparations of Examples 2, 3 and Comparative Examples 2 and 3 prepared in Formulation Examples 2 and 3 are respectively applied in two places, and the other one is compared. It was used as a control for. Each cosmetic was steadily applied for 8 weeks at intervals of 2 weeks, and after 8 weeks, the skin color (L value) was measured using Minolta CR 300, and then the change in skin color (ΔL) was calculated according to Equation 3 below. The results are shown in Table 8 below.

[Equation 3]

Skin color change (ΔL) = L value before applying cosmetics-L value after applying cosmetics

division Example 2 Example 3 Comparative Example 2 Comparative Example 3 Control ΔL value 0.83 1.02 0.23 0.32 0.00

As shown in Table 8, when looking at the ΔL value after 8 weeks, the cosmetic examples 2 and 3 of the present invention containing the butterfly bean flower extract were significantly higher than the control group. Therefore, it can be seen that the cosmetic composition of the present invention has a very good whitening effect.

Experimental Example 4: Cosmetic Skin irritation test of the composition

Skin irritation experiments of products prepared according to the above-mentioned Preparation Examples and Comparative Examples were conducted on 50 healthy adult men and women. A certain amount (about 0.1 g) of each sample (Examples 1 to 3 and 1 to 3) was sprinkled with 5 X 20 cm ² on the lower arm of each subject for 24 hours, removed, and visually observed after 1 hour and 24 hours elapsed. By confirming the change in skin condition, the degree of skin reaction was determined according to the skin reaction evaluation criteria in Table 9. In addition, the skin irritation rate was calculated according to Equation 4 below based on the determined result, and the results are shown in Table 10.

division Skin reaction degree No erythema or unusual phenomena - Slightly reddening than the surroundings -+ Significant redness than the surroundings + Reddening and swelling more severely than the surroundings ++

[Equation 4]

Formulation Judgment result (persons) Stimulation rate (%) ++ + +- - Example 1 - - 2 48 1.33 Example 2 - - 3 47 2.00 Example 3 - - 3 47 2.00 Comparative Example 1 - - 2 48 1.33 Comparative Example 2 - - 3 47 2.00 Comparative Example 3 - - 3 47 2.00

As can be seen from Table 10, the cosmetic compositions of Examples 1 to 3 of the present invention containing the butterfly bean flower extract have almost similar skin irritation rate compared to the cosmetic compositions of Comparative Examples 1 to 3 that do not contain the butterfly bean flower extract. By showing, it was found that the butterfly bean extract of the present invention hardly irritated the skin. Therefore, it was found that the cosmetic composition of the present invention has little skin irritation and excellent skin safety.

Experimental Example 5: Measurement of antioxidant effect

Thiobarbituric acid method (TBA assay) using the lipid peroxidation system by the Fenton reaction of antioxidant activity for Examples 1 to 3 and Comparative Examples 1 to 3 containing the butterfly bean flower extract of the present invention ). That is, 10 µl of ethyl linoleate, 0.2% sodium dodecyl sulfoxide (SDS), 10 µmol of ferric chloride, and 2 µmol of hydrogen peroxide containing 25 mM Trizma hydrochloride/0.75 mM potassium chloride buffer 5 ml containing butterfly bean extract After adding dimethyl sulfoxide (DMSO) test solution to Examples 1 to 3 and Comparative Examples 1 to 3, incubating at 55° C. for 16 hours, 50 μl of 4% dibutylhydroxytoluene (BHT) was added to stop the reaction. . 0.3 ml of this solution was put in a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and after centrifugation, the absorbance of the butanol layer was measured at 535 nm, and the antioxidant effect of each extract was calculated by the following equation (5). The results are shown in Table 11 below.

[Equation 5]

Test substance Antioxidative effect (%) Experimental concentration (10 μg/ml) Experimental concentration (50 μg/ml) Experimental concentration (100μg/ml) Example 1 15 45 65 Example 2 28 53 68 Example 3 30 55 72 Comparative Example 1 2.2 4.8 11.4 Comparative Example 2 7.2 11.8 19.2 Comparative Example 3 7.5 12.4 20.6

When the antioxidant effects of Examples 1 to 3 and Comparative Examples 1 to 3 were compared, Examples 1 to 3 containing the butterfly bean flower extract exhibited high activity in anti-oxidation effect.

Claims (4)

A cosmetic composition for skin whitening comprising butterfly bean extract as an active ingredient. According to claim 1, The butterfly flower extract is water, anhydrous or hydrous lower alcohol having 1-4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol is selected from the group consisting of solvents It is extracted by, cosmetic composition for skin whitening. According to claim 1, The butterfly flower extract is contained in 0.001 to 30% by weight based on the total weight of the composition of the cosmetic, cosmetic composition for skin whitening. According to claim 1, The butterfly flower extract is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray A cosmetic composition for skin whitening, which is a formulation selected from the group consisting of.