KR102692062B1 - A cosmetic composition having anti-oxidant, anti-inflammatory, protection of skin cell induced by ultraviolet ray and skin whitening effects comrising camellia sinensis extract, rumex crispus extract and inula britannica extract - Google Patents

Abstract

One aspect relates to a cosmetic composition having antioxidant, anti-inflammatory, ultraviolet ray-induced skin cell protection and skin whitening effects, comprising Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract. The cosmetic composition according to one aspect includes tea tree extract, Sorijani extract, and Geumbulcho extract, and has antioxidant effects through ABTS radical scavenging ability and DPPH radical scavenging ability compared to a single extract, anti-inflammatory effect through suppressing NO production, and protection of skin cells by ultraviolet rays. It has excellent effects in inhibiting tyrosinase activity and melanin production, preventing or improving skin oxidation (aging), skin troubles, and skin inflammation, and has excellent skin whitening effects. Therefore, the cosmetic composition can be usefully used in cosmetics for skin improvement.

Description

Cosmetic composition with antioxidant, anti-inflammatory, ultraviolet ray-induced skin cell protection and skin whitening effects containing tea tree extract, Sorijaengi extract and Geumbulcho extract {A COSMETIC COMPOSITION HAVING ANTI-OXIDANT, ANTI-INFLAMMATORY, PROTECTION OF SKIN CELL INDUCED BY ULTRAVIOLET RAY AND SKIN WHITENING EFFECTS COMRISING CAMELLIA SINENSIS EXTRACT, RUMEX CRISPUS EXTRACT AND INULA BRITANNICA EXTRACT}

It relates to a cosmetic composition containing antioxidant, anti-inflammatory, ultraviolet ray-induced skin cell protection, and skin whitening effects, including tea tree extract, Sorijani extract, and Geumbulcho extract.

The skin is the largest organ in our body and is exposed to the outside world, so it plays a role in protecting the body from external stimuli. Among various external stimuli, ultraviolet rays are known to be the main cause of photoaging. Depending on the wavelength, ultraviolet rays are divided into UVA (320 - 400 nm), UVB (280 - 320 nm), and UVC (200 - 280 nm). Among these, the ultraviolet rays that reach the earth's surface and affect the human body are known as UVA and UVB. there is. When exposed to UVB, some of it penetrates the epidermis and reaches the papillary layer of the dermis, directly causing skin damage such as skin aging, burns, and skin cancer. UVA is known to accelerate skin aging by damaging skin lipids in the epidermis or causing melanin pigment in the basal layer. Numerous epidemiological studies have demonstrated a strong relationship between sun exposure and human skin cancer; see DeSimone, "Sunscreen and Suntan Products", Handbook of Nonprescription Doctors, 7th Ed., Chapter 26, pp 499-511 ( American Pharmaceutical Association, Washington, D.C.; 1982). Accordingly, there is a need to develop various materials to protect the skin from ultraviolet rays by blocking UVA and UVB.

UVB mainly acts on the outermost epidermal layer of the skin, causing an inflammatory response through the secretion of cytokines and chemokines, especially UVB-induced tumor necrosis factor-alpha (TNF). Pro-inflammatory cytokines such as -α), interleukin-6 (IL-6), and interleukin-8 (IL-8) are known to contribute to skin damage. In addition, UVB causes the production of reactive oxygen species (ROS). ROS continuously generated in keratin cells are quickly removed by enzymatic and non-enzymatic methods, but excessively generated ROS causes skin damage. , Free radicals generated from ROS cause deterioration of cell function, resulting in abnormal gene expression.

Free radicals lead to damage to skin cells and tissues, destroying the antioxidant defense system, contributing to not only biological aging but also skin aging, and cause functional damage to various organs, causing various diseases. In addition, continuous oxidative stress causes damage to biological components such as activation of proteolytic enzymes, cleavage of elastic fibers collagen and elastin, and cleavage of hyaluronic acid, causing skin inflammation and suppressing skin immune function, resulting in an increase in the rate of bacterial infection or carcinogenesis. Therefore, skin cells must be protected by suppressing the production of free radicals and suppressing the inflammatory response caused by free radicals.

In order to protect skin cells from ultraviolet rays, it is important to protect cells from free radicals and DNA damage. Recently, research has been actively conducted to protect skin cells from ultraviolet rays while being safer using various herbal medicines or edible plant extracts.

These days, people are affected not only in terms of skin health but also in terms of aesthetics, such as skin darkening, pigmentation, skin aging, and skin cancer due to excessive melanin deposition due to long-term exposure to harmful ultraviolet rays due to the increase in social activities and the destruction of the ozone layer due to environmental pollution. . Darkening of the skin is determined by the content of melanin produced by melanocytes present in the basal layer. Melanin is a biopolymer that exists widely in nature and plays a role in preventing skin damage from ultraviolet rays or free radicals. there is.

As the mechanism of melanin production has been revealed, methods of mixing substances that have an effect of inhibiting the activity of tyrosinase, an enzyme involved in the melanin production process, into cosmetics have been commonly used. Representative substances used for this purpose include ascorbic acid, kojic acid, hydroquinone, and plant extracts such as mulberry extract and licorice extract. However, while kojic acid has a high tyrosinase activity inhibitory effect, it has stability problems such as discoloration and a decrease in potency due to changes over time when mixed in cosmetics, and has limitations in use due to its large skin irritation. Ascorbic acid has tyrosinase activity. It is not suitable due to its low inhibitory effect and low stability of the molecule itself, and hydroquinone is highly irritating to the skin, so its usability in cosmetic formulations is currently limited due to safety issues.

Accordingly, there is a growing demand for cosmetic compositions that solve the above problems, are biocompatible, can reduce skin damage caused by ultraviolet rays, and exhibit excellent whitening activity.

The steam extraction method used in the present invention has the advantage of creating new compounds by changing the constituents using steam, decomposing high molecular compounds to create low molecular substances, and destroying tissues to maximize the elution of useful components. In general, the heat treatment process burns plant tissue components and destroys active ingredients, reducing yield and physiological activity. To compensate for these shortcomings, a steam extraction method that can increase the elution of useful components by using steam, which is an indirect heat treatment rather than direct heat treatment, is being used.

One aspect is to provide a cosmetic composition comprising Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract.

Another aspect is to provide a method for producing a cosmetic composition comprising mixing Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract.

One aspect provides a cosmetic composition comprising Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract.

"Tea tree ( Camellia Sinensis )" is an evergreen broad-leaved shrub that is mainly used for green tea in Korea and black tea in places such as India and Sri Lanka. Meanwhile, in China, the leaves of the tea tree are also used as " Puer Tea ", which refers to a fermented tea mainly originating from the Yunnan region of China. Pu-erh tea is mainly obtained from large-leaved tea trees that grow naturally in Yunnan Province, China. It is a tea made by destroying the enzymes of the tea leaves and then depositing the tea leaves to induce the reproduction of microorganisms in the air and fermentation again. Ingredients contained in tea leaves include vitamins, caffeine, tannin, flavonoids, and essential oils (Yongju Lee et al., Herbal Pharmacology, gerund. 189-190, 1981). Tea leaves are traditionally known to have anti-diabetic, obesity, antioxidant, antihypertensive, antibacterial, cholesterol-lowering and anti-ulcer effects, and are therefore routinely consumed as commercial tea.

Meanwhile, Rumex Crispus is a perennial plant belonging to the Nodule family. It is a plant that is native to Europe in the Northern Hemisphere and is distributed all over the world, having spread to the Southern Hemisphere as well. The root of the above Sorujaengi is called " Rumex japonicus Houttuyn ", and is also called Sorujaengi, Soljaengi, Songguji, etc. It is a perennial plant in the knotweed family whose stems dry up in midsummer and grow again when the weather cools. Young leaves are eaten as a vegetable and are effective for ringworm, athlete's foot, and eczema. Since ancient times, it has been used in the private sector for skin diseases such as scabies, scabies, and skin cancer, and it is also known to have been used in decoction for cystitis and colitis. The roots that are dug up and dried in the fall are called Yangjegeun. It is known that Yangje root extract is very effective in suppressing the secretion and action of elastase and hyaluronidase, and has an excellent anti-inflammatory effect through suppressing the secretion of inflammatory cytokines such as IL-8 (Interleukin-8). .

In addition, “Geumbulcho ( Inula britannica )” is a perennial herb that grows in the mountains and fields of various parts of Korea. It grows well in moist places in the mountains and fields, and is a semi-shade or sunny plant. It is 20 to 60 cm tall, and the leaves are 5 to 10 cm long and 1 to 3 cm wide, alternate, long oval shaped, and have sparse sawtooth edges. The flowers are yellow and grow at the ends of branches and stems and are about 3 cm in diameter. The fruits grow around October and have caps about 0.5 cm long. Unlike other chrysanthemums, the flower is characterized by narrow and long petals. The young shoots are edible and the flowers are also used for medicinal purposes. The flower of Geumbulcho is also called Seonbokhwa.

According to existing research, a compound isolated from the extract of Seonbokhwa (Geumbulcho flower) produces cyclooxygenase-2 (COX-2)-dependent prostaglandins and leukotrienes ( It is known that it can be usefully used as a medicine or health functional food for the prevention and treatment of various inflammatory or allergic diseases because it inhibits the production of leukotriene and at the same time inhibits the release of histamine from mast cells.

In addition, according to existing research, it is known that Geumbulcho flower extract has antibacterial activity against skin flora, has antioxidant activity, and tyrosinase inhibitory activity, so it has the potential to be applied as a functional cosmetic raw material.

In one aspect, the tea tree, sorcerer and/or Geumbulcho may be all of them, a portion thereof, or materials derived from them. In addition, tea tree, sorijagi and/or Geumbulcho may be whole, part thereof, or materials derived from them may be ground or suitably dried. Additionally, products with a moisture content of less than 20% can be used after drying.

Specifically, in one aspect, the tea plant may be one or more selected from the group consisting of roots, stems, leaves, and flowers of the tea plant, and specifically, the tea plant may be leaves of the tea plant.

In addition, the sorrel may be one or more selected from the group consisting of the roots, stems, leaves, and flowers of the sorrel, and specifically, the sorrel may be the root of the sorrel.

In addition, the Geumbulcho may be one or more selected from the group consisting of roots, stems, leaves, and flowers of Geumbulcho, and specifically, the Geumbulcho may be a flower of Geumbulcho.

In one aspect, the tea tree, the sorcerer, and/or the Geumbulcho may be steamed, or, more specifically, may be dried after steaming.

The term “extract” refers to a result such as a liquid component obtained by immersing the desired substance in various solvents and then extracting it at room temperature or at a heated state for a certain period of time, and a solid content obtained by removing the solvent from the liquid component. In addition, in addition to the above results, it can be comprehensively interpreted to include dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results. Accordingly, the extract according to one aspect includes the extract itself and the extract, such as an extract obtained by extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude product or purified product of the extract, or a mixture thereof. It can be interpreted as including extracts of all formulations that can be formed using .

In one aspect, the Camellia sinensis extract, the Sorija extract, or the Geumbulcho extract are extracted with one or more selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol. It may be, specifically, it may be extracted with a mixture of alcohol and water having 1 to 5 carbon atoms, that is, an aqueous alcohol solution, and more specifically, it may be extracted with a mixture of ethanol and water, that is, an aqueous ethanol solution.

In addition, the alcohol concentration of the mixture of alcohol and water having 1 to 5 carbon atoms is 1 to 100% (w/w), for example, 10 to 100% (w/w), 20 to 100% (w/w), 30 to 100% (w/w), 40 to 100% (w/w), 50 to 100% (w/w), 60 to 100% (w/w), 70 to 100% (w/w), It may be 60 to 90% (w/w), 60 to 80% (w/w).

In one aspect, the extraction method of the extract is not particularly limited, and may be extracted alone or by combining two or more methods according to methods commonly used in the art. Non-limiting examples of the extraction method include heating extraction, hot water extraction, cold needle extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, compression, subcritical extraction, and supercritical extraction.

In one aspect, the composition may have one or more activities selected from the group consisting of antioxidant, anti-inflammatory, skin cell protection, and skin whitening.

The "antioxidant" refers to the activity of preventing and improving the cellular aging process and skin oxidation process by removing active oxygen, which is the main cause of skin aging, and is tested for phenol content measurement test, flavonoid content measurement test, DPPH ( It can be measured through 2,2-diphenylpicrylhydrazyl) radical scavenging and ABTS (2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt) radical scavenging.

According to one aspect, the cosmetic composition may improve skin oxidation, that is, have a skin antioxidant effect, and may have the effect of preventing or improving skin aging. At this time, the skin aging may be one or more selected from the group consisting of wrinkles, decreased skin elasticity, blemishes, freckles, skin damage caused by ultraviolet rays, skin dryness, weakened skin barrier, weakened skin transparency, keratinization, damaged skin texture, and skin cancer. It may be one or more selected from the group consisting of wrinkles, skin damage caused by ultraviolet rays, decreased skin elasticity, skin dryness, weakened skin barrier, weakened skin transparency, keratinization, and damaged skin texture, and more specifically, It may be one or more selected from the group consisting of skin damage caused by ultraviolet rays, wrinkles, and decreased skin elasticity.

According to one aspect, the antioxidant activity may be a radical scavenging activity. Specifically, the cosmetic composition according to one aspect includes DPPH (1-1-diphenyl-2-picryl-hydrazyl) radical and ABTS (2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt. ) It has excellent radical scavenging ability, showing excellent skin antioxidant effect.

In addition, the term "anti-inflammatory" refers to the activity of suppressing inflammation, and the control of inflammatory response is known to be very complex, and is known to enhance the in vivo repair system and reduce damage. However, if the inflammatory response continues due to repeated tissue damage or regeneration, ROS and RNS are excessively produced in inflammation-related cells, resulting in permanent genetic modification. In this way, ROS and RNS are deeply related to the inflammatory response that regulates the actions of various cells in vivo. Major inflammatory cytokines include tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), IL-6, and IL-8.

In particular, the cosmetic composition according to one aspect may exhibit anti-inflammatory activity against skin inflammation. In addition, the skin inflammation is selected from the group consisting of dermatitis, allergic dermatitis, irritant dermatitis, seborrheic dermatitis, atopic dermatitis, sensitive skin disease, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, drug rash, and acne. There can be more than one.

According to one aspect, the anti-inflammatory activity may be one or more selected from the group consisting of NO production inhibition ability and inflammatory cytokine inhibition ability, and specifically, may be NO production inhibition ability.

Additionally, in one aspect, the inflammatory cytokine may be one or more selected from the group consisting of tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), IL-6, and IL-8, Specifically, it may be TNF-α (tumor necrosis factor-α).

Specifically, the cosmetic composition according to one aspect has an excellent ability to inhibit NO (nitric oxide) production, and thus exhibits an excellent skin inflammation improvement effect.

The term “skin cell protection” refers to the activity of protecting or soothing skin cells against skin irritation, and the skin irritation may be, for example, irritation caused by dust, fine dust, compounds, inflammation, ultraviolet rays, etc. Protective or soothing activity of skin cells means alleviating heat or pain in irritated skin, and includes soothing skin irritated by skin inflammation.

In one aspect, the cosmetic composition may prevent or improve one or more conditions selected from the group consisting of skin trouble, skin aging, skin inflammation, skin heat, and skin pain, and specifically, skin trouble, skin aging, and skin pain. It may be to prevent or improve both inflammation, hot skin, and skin pain.

The term “prevention” may refer to any action that suppresses or delays skin trouble, skin aging, skin inflammation, skin heat, and/or skin pain in an individual by applying the cosmetic composition according to one aspect.

The term “improvement” may refer to any action that results in at least a reduction in the severity of a parameter associated with the condition being treated, such as symptoms. At this time, the cosmetic composition is used before the progression of the skin trouble, skin aging, skin inflammation, skin heat and/or skin pain in order to prevent or improve skin trouble, skin aging, skin inflammation, skin heat and/or skin pain. Thereafter, it can be used simultaneously with or separately from the medicament for treatment.

Specifically, the cosmetic composition according to one aspect has an excellent cell protection effect against damage to skin cells caused by ultraviolet rays, especially UVA, and is excellent for preventing skin trouble, skin aging, skin inflammation, skin heat, and/or skin pain. Shows improvement effect.

The “skin whitening” activity is induced by inhibiting the activity of an enzyme called tyrosinase or promoting the reduction of already produced melanin pigment.　 Tyrosine produced in melanosomes, melanocytes present in the epidermis, the surface of the skin, is a precursor of melanin and is synthesized into melanin through DOPA-quinone. In this intermediate process, an enzyme called tyrosinase acts. When the activity of tyrosinase, which is involved in the process of oxidation of tyrosine into dopa and dopachrome, is inhibited, melanin production is suppressed, thereby improving skin tone. A brightening and whitening effect appears.

In one aspect, the skin whitening activity may be one or more selected from the group consisting of tyrosinase activity inhibition ability and melanin production inhibition ability, and specifically, may be both tyrosinase activity inhibition ability and melanin production inhibition ability.

The cosmetic composition according to one aspect is excellent in both the efficacy of inhibiting tyrosinase activity and inhibiting melanin production, and thus exhibits excellent skin whitening activity.

In one aspect, the composition may include the Camellia sinensis extract, the Camellia sinensis extract, and the Geumbulcho extract in a weight ratio of 0.1 to 3:0.1 to 3:0.1 to 3. Specifically, the composition is composed of the tea tree extract, the Camellia sinensis extract, and the Geumbulcho extract in a ratio of 1:1:1, 2:1:1, 1:2:1, 1:1:2, 2:2:1, 2. : 1 : 2, 1 : 2 : 2 or 2 : 2 : 2, or 2 : 2: 2, and more specifically, the composition may contain the tea tree extract, the Sorijangi extract, and the Geumbulcho extract at a weight ratio of 2: 1: 1. It may be included by weight ratio.

When the weight ratio of the Camellia sinensis extract, the Sorijani extract, and the Geumbulcho extract in the composition is within the above range, the composition has a synergistic effect that exhibits superior antioxidant, anti-inflammatory, skin cell protection, and skin whitening activities compared to the single extract, If the weight ratio of the Camellia sinensis extract, the Sorija extract, and the Geumbulcho extract in the composition is outside the above range, other synergistic effects of mixing the extracts will be reduced, and the antioxidant, anti-inflammatory, skin cell protection, and skin whitening activities will be reduced. You can.

According to one aspect, the composition may be extracted after mixing the tea tree, Sorijani and Geumbulcho. In general, when each raw material is mixed and then extracted, the existing efficacy may be impaired. However, according to one aspect, in the case of a composition prepared by mixing and extracting tea tree, Sorijani and Geumbulcho, the antioxidant, anti-inflammatory, skin cell protection and skin whitening activities can be increased compared to the single extract of the raw material.

In addition, according to one aspect, the composition may be a mixture of extracts from each of Tea Tree, Sorijani and Geumbulcho. When preparing a composition by extracting each raw material and then mixing the extracted extracts, not only can the efficacy of the raw material be maintained, but compared to producing an extract by mixing and then extracting the raw materials, it is possible to achieve antioxidant, anti-inflammatory, skin cell protection and Skin whitening activity may be increased.

In one aspect, each of the Camellia sinensis extract, the Sorijani extract and the Geumbulcho extract is present in an amount of 0.0001% to 99.0% by weight, for example, 0.01% to 60% by weight, 0.01% to 40% by weight, based on the total weight of the composition. , 0.01% to 30% by weight, 0.01% to 20% by weight, 0.01% to 10% by weight, 0.01% to 5% by weight, 0.05% to 60% by weight, 0.05% to 40% by weight, 0.05% by weight. Weight % to 30 weight %, 0.05 weight % to 20 weight %, 0.05 weight % to 10 weight %, 0.05 weight % to 5 weight %, 0.1 weight % to 60 weight %, 0.1 weight % to 40 weight %, 0.1 weight % to 30% by weight, 0.1% to 20% by weight, 0.1% to 10% by weight, 0.1% to 5% by weight, 5 to 20% by weight, 5 to 18% by weight, 6 to 15% by weight, 8 to 15% by weight It may be included in weight% or 7 to 10 weight%.

Specifically, in one aspect, the concentration of the Camellia sinensis extract, the Camellia sinensis extract, and the Geumbulcho extract in the composition may be 0.001 to 1% by weight, and more specifically, the concentration of the Camellia sinensis extract, the Camellia sinensis extract, and the extract of the Geumbulcho extract in the composition. is 0.001 to 1% by weight, 0.001 to 0.1% by weight, 0.001 to 0.05% by weight, 0.005 to 1% by weight, 0.005 to 0.1% by weight, 0.005 to 0.05% by weight, 0.01 to 1% by weight, 0.01 to 0.1% by weight or 0.01% by weight. It may be from 0.05% by weight.

When the concentration of the Camellia sinensis extract, the Sorija extract, and the Geumbulcho extract in the composition are within the above range, the composition has a synergistic effect that exhibits superior antioxidant, anti-inflammatory, skin cell protection, and skin whitening activities compared to the single extract, If the concentration of the Camellia sinensis extract, the Sorija extract, and the Geumbulcho extract in the composition is outside the above range, other synergistic effects of mixing the extracts will be reduced, and the antioxidant, anti-inflammatory, skin cell protection, and skin whitening activities will be reduced. You can. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in amounts above the above range.

The term "cosmetic composition" refers to an article used on the human body to clean and beautify the human body, add attractiveness, brighten the appearance, or maintain or promote the health of the skin or hair.

In one aspect, the cosmetic composition can be manufactured in any formulation commonly manufactured in the technical field to which the present invention pertains. For example, the composition may be a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing agent, oil, powder foundation, emulsion foundation, wax foundation, massage cream, essence, ampoule. , skin adhesive type, spray, etc. Specifically, lotions such as softening lotions or nutritional lotions, emulsions such as facial lotions and body lotions, creams such as nutritional creams, moisture creams, eye creams, essences, cosmetic ointments, sprays, gels, packs, sunscreens, and makeup bases. , foundation such as liquid type, solid type or spray type, makeup remover such as powder, cleansing cream, cleansing lotion, cleansing oil, cleansing agent such as cleansing foam, soap, body wash, etc., liquid or mesotherapy that can be injected into the skin layer. It can be prepared in formulations such as gel formulations and formulations mixed with hyaluronic acid gel.

The cosmetic composition includes additional ingredients commonly used in cosmetics, such as excipients, thickeners, dispersants, fragrances, fillers, preservatives, preservatives, neutralizers, sweeteners, vitamins, free radical scavengers, metal ion sequestrants, functional ingredients, and these. It may further comprise any conventional cosmetic ingredient which may be selected from a mixture of. One of ordinary skill in the art can select any additional ingredients and/or amounts thereof such that the advantageous properties of the composition according to one aspect are not or substantially not adversely affected by the envisaged additions.

For example, the excipients include surfactants, emulsifiers, soapy acid, solvents, colorants, preservatives, antioxidants, anti-foaming agents, antibacterial agents, anti-redeposition agents, enzymes, vegetable or mineral oils, fats, fluorescent substances, fungicides, and hydrotropes. , moisturizers, fragrances, preservatives, proteins, silicones, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, waxes, fatty acids, higher alcohols, hydrocarbon oils, ester oils, triglycerides, vegetable oils, etc. there is.

According to one aspect, the cosmetic composition contains ingredients commonly added to cosmetic compositions, such as purified water, surfactants, humectants, lower alcohols, chelating agents, disinfectants, preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. It may additionally include common auxiliaries such as.

In addition, the cosmetic composition generally contains ingredients used in external skin preparations such as cosmetics and medicines, such as water-based ingredients, oil-based ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, and surfactants. , fragrance, colorant, various skin nutrients, or a combination thereof may be appropriately mixed according to need. The cosmetic composition also contains metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glablidin, and calin. hot water extract of fruits, various herbal medicines, drugs such as tocopherol acetate, glythylitic acid, tranexamic acid and its derivatives or salts, vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, kojic acid, glucose, fructose Sugars such as , trehalose, etc. can also be appropriately mixed.

When the cosmetic composition is a surfactant-containing cleansing formulation, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid mono ester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide ether. It may further include sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester.

In addition, when the cosmetic composition according to one aspect is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be added as a carrier ingredient. It can be included.

In addition, when the cosmetic composition is in the form of a solution or emulsion, the solvent, solvating agent, or emulsifying agent may be water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol, or sorbitan. It may further include fatty acid esters, etc.

When the cosmetic composition is a suspension formulation, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystalline It may further include cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

When the cosmetic composition is in a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder as a carrier ingredient. In particular, in the case of a spray formulation, it may additionally include chlorofluorohydrocarbon. , may further include a propellant such as propane/butane or dimethyl ether.

Additionally, in one aspect, the cosmetic composition may further include an organic sunscreen and/or an inorganic sunscreen.

The organic sunscreen includes ethylhexylmethoxycinnamate, Bis-ethylhexyloxyphenol methoxyphenyltriazine, ethylhexyl salicylate, butylmethoxydibenzoylmethane, octocrylene, homosalate, and isoamyl p-methane. It may be toxin cinnamate, diethylaminohydroxybenzoylhexyl benzoate, phenylbenzimidazole sulfonic acid, etc., and the inorganic sunscreen may be titanium dioxide, zinc oxide, zinc oxide, etc.

The sunscreen may be included in an amount of 1 to 50% by weight, 5 to 45% by weight, 5 to 40% by weight, 10 to 30% by weight, or 15 to 25% by weight, based on the total weight of the cosmetic composition.

The cosmetic composition may be used singly or in combination, or may be used in combination with other cosmetic compositions other than the cosmetic composition according to one aspect. Additionally, all ingredients included in the cosmetic composition do not exceed the regulations set by each country. In addition, the cosmetic composition according to one aspect may be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or taste.

Another aspect provides a method for producing a cosmetic composition comprising mixing Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract.

The "Tea tree extract", "Sorijaengi extract", "Geumbulcho extract", "cosmetic composition", etc. may be within the above-mentioned range.

According to one aspect, the method includes ultrasonic extraction of the tea tree to obtain a tea tree extract;

Obtaining a tea tree filtrate by filtering the tea tree extract;

Concentrating the tea tree filtrate to obtain a tea tree concentrate;

Obtaining tea tree extract powder by removing the solvent from the tea tree concentrate; and

It may further include mixing the tea tree extract powder with a solvent to obtain the tea tree extract.

Additionally, in one aspect, the method may further include steaming the tea tree prior to obtaining the tea tree extract.

Additionally, in one aspect, the method includes the steps of ultrasonic extraction of the snail extract to obtain a snail extract;

Obtaining a sorrel filtrate by filtering the sorrel extract;

Concentrating the filtrate to obtain a filtrate concentrate;

Obtaining a Sorijaeng extract powder by removing the solvent from the Sorijaengi concentrate; and

It may further include the step of obtaining the Sorijagi extract by mixing the Sorijagi extract powder with a solvent.

Additionally, in one aspect, the method may further include the step of steaming the sorrel before obtaining the extract of the sorrel.

In addition, the method includes the steps of ultrasonic extraction of Geumbulcho to obtain Geumbulcho extract;

Obtaining Geumbulcho filtrate by filtering the Geumbulcho extract;

Concentrating the Geumbulcho filtrate to obtain Geumbulcho concentrate;

Obtaining Geumbulcho extract powder by removing the solvent from the Geumbulcho concentrate; and

It may further include the step of mixing the Geumbulcho extract powder with a solvent to obtain the Geumbulcho extract.

Additionally, according to one aspect, the method may further include the step of steaming the Geumbulcho extract prior to the step of obtaining the Geumbulcho extract.

The ultrasonic extraction is performed at 4 to 70°C, for example, 4 to 50°C, 4 to 40°C, 4 to 30°C, 10 to 70°C, 15 to 70°C, 20 to 70°C, 10 to 50°C, 10 to 70°C. It may be performed at 40°C, 10 to 35°C, 10 to 30°C, or room temperature.

In one aspect, the ultrasonic extraction may be performed with one or more selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol, and specifically, alcohol having 1 to 5 carbon atoms. 5, it may be performed with a mixture of alcohol and water, that is, an alcohol aqueous solution, and more specifically, it may be performed with a mixture of ethanol and water, that is, an ethanol aqueous solution.

In addition, the alcohol concentration of the mixture of alcohol and water having 1 to 5 carbon atoms is 1 to 100% (w/w), for example, 10 to 100% (w/w), 20 to 100% (w/w), 30 to 100% (w/w), 40 to 100% (w/w), 50 to 100% (w/w), 60 to 100% (w/w), 70 to 100% (w/w), It may be 60 to 90% (w/w), 60 to 80% (w/w).

In addition, the ultrasonic extraction is performed by increasing the extraction solvent by 3 to 50 (w/w) times, for example, 3 to 40 (w/w) times, 3 to 30 (w) times, with respect to the tea tree, cypress and/or Geumbulcho. /w) times, 5 to 50 (w/w) times, 5 to 40 (w/w) times, 5 to 30 (w/w) times, or 4 to 40 (w/w) times. You can. For example, it may be carried out by adding 3 to 50 kg of the extraction solvent per 1 kg of material derived from the tea tree, Sorija and/or Geumbulcho.

In one aspect, the ultrasonic extraction time may vary depending on the selected temperature, from 1 hour to 2 months, for example, 1 hour to 1 month, 1 hour to 10 days, 1 hour to 5 days, 1 hour to 3 days. , 1 hour to 2 days, 1 hour to 1 day, 1 hour to 10 hours, 2 hours to 1 month, 2 hours to 15 days, 2 hours to 10 days, 2 hours to 5 days, 2 hours to 3 days, 2 It can be from 1 hour to 2 days, 2 hours to 1 day, or 2 hours to 10 hours. In addition, the ultrasonic extraction may include mixing the whole, part of, or materials derived from the Camellia sinensis, L. chinensis and/or Geumbulcho, in the solvent and leaving it for a certain period of time. The standing may include moderate agitation. The ultrasonic extraction may be repeated one or more times, for example, 1 to 5 times.

Additionally, the filtration may be performed by a normal filtration method, and specifically may be performed using a mesh filter. Additionally, the concentration may be performed by a conventional concentration method, for example, reduced pressure concentration.

In addition, the method may further include the step of drying the Camellia sinensis extract, the Sori extract, and/or the Geumbulcho extract, for example, the drying is performed by a conventional drying method such as freeze-drying and spray drying. may be performed, for example, in a vacuum.

According to one aspect, through the drying step, the Camellia sinensis extract, the Sorija extract, and/or the Geumbulcho extract can be obtained in powder form.

In one aspect, the method includes steaming the tea tree prior to obtaining the tea tree extract, steaming the tea tree prior to obtaining the tea tree extract, and/or prior to obtaining the Geumbulcho extract. The step of steaming the Geumbulcho may be further included. Additionally, according to one aspect, the steaming step may include a drying step after steaming.

Additionally, according to one aspect, any device used in the steaming step may be used as long as it can be steamed at a constant temperature for a certain period of time. Specifically, the steaming step may be performed at 100 to 140°C, specifically 110 to 130°C, more specifically 115 to 125°C, and may be performed for 1 to 8 hours or 1 to 6 hours, specifically 2 to 8 hours. It can be. Meanwhile, the drying step after steaming may be performed at 40 to 80°C for 1 to 12 hours, specifically at 50 to 70°C for 2 to 10 hours.

Additionally, in one aspect, the mixing step may be mixing the Camellia sinensis extract, the Camellia sinensis extract, and the Geumbulcho extract at a weight ratio of 0.1 to 3:0.1 to 3:0.1 to 3. Specifically, the mixing step is performed by mixing the tea tree extract, the sorijangi extract, and the Geumbulcho extract at a ratio of 1:1:1, 2:1:1, 1:2:1, 1:1:2, 2:2:1. , may be mixing at a weight ratio of 2:1:2, 1:2:2 or 2:2:2, and more specifically, the mixing step may be performed by mixing the Camellia sinensis extract, the Sorijani extract, and the Geumbulcho extract in a ratio of 2: It may be mixed at a weight ratio of 1:1.

In addition, in the mixing step, the solvent is used to dissolve the extraction powder, for example, from the group consisting of water, alcohol with 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol. It may be one or more selected substances, and specifically, it may be a mixture of alcohol and water having 1 to 5 carbon atoms, that is, an aqueous alcohol solution, and more specifically, it may be a mixture of ethanol and water, that is, an aqueous ethanol solution.

In addition, the alcohol concentration of the mixture of alcohol and water having 1 to 5 carbon atoms is 1 to 100% (w/w), for example, 10 to 100% (w/w), 20 to 100% (w/w), 30 to 100% (w/w), 40 to 100% (w/w), 50 to 100% (w/w), 60 to 100% (w/w), 70 to 100% (w/w), It may be 60 to 90% (w/w), 60 to 80% (w/w).

In the mixing step, when the mixing ratio of the tea tree extract, the sorrel extract, and the Geumbulcho extract is within the above range, the composition exhibits a synergistic effect that exhibits superior antioxidant, anti-inflammatory, skin cell protection, and skin whitening activities compared to the single extract. In the mixing step, if the mixing ratio of the tea tree extract, the sorrel extract, and the Geumbulcho extract is outside the above range, other synergistic effects of the mixing of the extracts are reduced, thereby reducing antioxidant, anti-inflammatory, skin cell protection, and skin whitening properties. Activity may decrease.

The cosmetic composition according to one aspect includes tea tree extract, Sorijani extract, and Geumbulcho extract, and has antioxidant effects through ABTS radical scavenging ability and DPPH radical scavenging ability compared to a single extract, anti-inflammatory effect through suppressing NO production, and protection of skin cells by ultraviolet rays. It has excellent effects in inhibiting tyrosinase activity and melanin production, preventing or improving skin oxidation (aging), skin troubles, and skin inflammation, and has excellent skin whitening effects. Therefore, the cosmetic composition can be usefully used in cosmetics for skin improvement.

Figure 1 is a diagram showing the results of tyrosinase inhibition experiments in Examples and Comparative Examples.
Figure 2 is a diagram showing the results of melanin production inhibition experiments in Examples and Comparative Examples.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Manufacturing example

Preparation Example 1. Preparation of tea tree leaf extract

Dried tea tree ( Camellia Sinensis ) leaves (pu'er tea) were purchased and used, and the raw material was steamed at 100°C for 2 hours and then dried at 70°C for 6 hours. The dried raw material was subjected to ultrasonic extraction for 3 hours with 10 times the weight of 70% alcohol. Afterwards, the extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 400 mesh), concentrated under reduced pressure using a rotary evaporator (R-100, Buchi, 1 L), and freeze-dried to prepare it in powder form. . At this time, the prepared extract was transferred to a sterilized container (KRS A-1, health up, Korea), refrigerated at 4°C, and used in the experiment.

Preparation Example 2. Preparation of Sorigi root extract

Dried Rumex Crispus roots were purchased and used. The raw material was steamed at 100°C for 2 hours and then dried at 70°C for 6 hours. The dried raw material was subjected to ultrasonic extraction for 3 hours with 10 times the weight of 70% alcohol. Afterwards, the extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 400 mesh), concentrated under reduced pressure using a rotary evaporator (R-100, Buchi, 1 L), and freeze-dried to prepare it in powder form. . At this time, the prepared extract was transferred to a sterilized container (KRS A-1, health up, Korea), refrigerated at 4°C, and used in the experiment.

Preparation Example 3. Preparation of Geumbulcho flower extract

Dried Inula Britannica flowers were purchased and used, and the raw material was steamed at 100°C for 2 hours and then dried at 70°C for 6 hours. The dried raw material was subjected to ultrasonic extraction for 3 hours with 10 times the weight of 70% alcohol. Afterwards, the extract was filtered through a mesh filter (stainless steel filter mesh, R&K, China, 400 mesh), concentrated under reduced pressure using a rotary evaporator (R-100, Buchi, 1 L), and freeze-dried to prepare it in powder form. . At this time, the prepared extract was transferred to a sterilized container (KRS A-1, health up, Korea), refrigerated at 4°C, and used in the experiment.

Examples and Comparative Examples

Preparation of Examples 1 to 3 (mixtures containing Camellia sinensis leaf extract, Camellia sinensis root extract and Geumbulcho flower extract) and Comparative Examples 1 to 3 (each of Camellia sinensis leaf extract, Sorania root extract and Geumbulcho flower extract)

The Camellia leaf extract of Preparation Example 1, the root extract of Preparation Example 2, and the flower extract of Preparation Example 3 were prepared using a 70% (w/w) ethanol aqueous solution in the composition shown in Table 1 below (Example: Examples 1 to 3).

In addition, in order to confirm the activity of the solution containing each extract alone, the Camellia sinensis leaf extract, the root extract of the root and the flower extract of Geumbulcho were each dissolved in the 70% (w/w) ethanol aqueous solution, and the extracts were contained alone. Comparative Examples 1 to 3 were also prepared.

At this time, to confirm the optimal mixing ratio, each extract was mixed according to the composition shown in Table 1 below, and the extract was stored in a sterilized container and refrigerated.

division Pu'er Tea Jegeun Yang golden fire flower Comparative Example 1 One - - Comparative Example 2 - One - Comparative Example 3 - - One Example 1 2 One One Example 2 One 2 One Example 3 One One 2

Experiment example

Experimental Example 1. Confirmation of antioxidant effect (DPPH assay)

In this experimental example, in order to confirm the antioxidant effect of Examples 1 to 3, the DPPH radical scavenging ability was evaluated.

Specifically, 100 μl of a 0.1 mM DPPH solution was added to 50 μl of the mixed extract at a concentration of 50 μg/ml, and then reacted at room temperature for about 10 minutes, shielded from light, and the absorbance was measured at 517 nm. Alpha-tocopherol was used as a control (PC, positive control), and the DPPH radical scavenging ability was calculated using Equation 1 below, and the results are shown in Table 2 below:

[Equation 1]

Inhibition (%) =｛1 - OD _sample / OD _control ｝Х 100.

division DPPH radical scavenging ability (%) Comparative Example 1 49.6 Comparative Example 2 31.2 Comparative Example 3 34.0 Example 1 62.3 Example 2 41.3 Example 3 46.1

As shown in Table 2, the DPPH radical scavenging ability of Examples 1 to 3 showed antioxidant activity superior to that of Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the most excellent DPPH radical scavenging ability among Examples 1 to 3 at about 62%.

Experimental Example 2. Confirmation of antioxidant effect (ABTS assay)

In this experimental example, the ABTS radical scavenging ability was evaluated to confirm the antioxidant effect of Examples 1 to 3. After adding 100 μl of the ABTS solution to 100 μl of the mixed extract at a concentration of 10 μg/ml, the mixture was incubated at room temperature for about 10 minutes, shielded from light, and the absorbance was measured at 734 nm. Ascorbic acid was used as a control (PC, positive control), and the ABTS radical scavenging ability was calculated using Equation 2 below, and the results are shown in Table 3 below:

[Equation 2]

Inhibition (%) =｛1 - OD _sample / OD _control ｝Х 100.

division ABTS radical scavenging ability (%) Comparative Example 1 57.7 Comparative Example 2 35.2 Comparative Example 3 44.4 Example 1 73.5 Example 2 41.4 Example 3 49.7

As shown in Table 3, the ABTS radical scavenging ability of Examples 1 to 3 showed antioxidant activity superior to that of Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the most excellent ABTS radical scavenging ability among Examples 1 to 3 at about 74%.

Experimental Example 3. Confirmation of anti-inflammatory effect (NO production inhibition ability)

In this experimental example, in order to confirm the anti-inflammatory effect of Examples 1 to 3, the ability to inhibit NO production was confirmed.

Specifically, Raw 264.7 cells, which are mouse macrophages, were distributed in a 96 well plate at a density of 1 Х 10 ⁵ cells/well and cultured for 24 hours. Afterwards, to induce an inflammatory response, the cells were treated with new medium containing 0.5 μg/ml concentration of lipopolysaccharide (LPS) and test substances and cultured for 24 hours. 50 μl each of the supernatant and Griess reagent were mixed, shielded from light, and reacted at room temperature for 10 minutes. After 10 minutes, the ability to inhibit NO production was measured through ELISA measurement at 540 nm. Quercetin was used as a control (PC, positive control), and the NO production inhibition rate (%) was calculated using Equation 3 below, and the results are shown in Table 4 below:

[Equation 3]

NO production inhibition rate (%) = ｛1 - OD _sample / OD _control ｝Х 100.

division NO production inhibition ability (%) Comparative Example 1 31.1 Comparative Example 2 25.5 Comparative Example 3 17.5 Example 1 49.4 Example 2 35.5 Example 3 29.7

As shown in Table 4, Examples 1 to 3 showed superior NO production inhibition ability than Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the most excellent NO production inhibition ability among Examples 1 to 3 at about 50% and exhibited anti-inflammatory activity.

Experimental Example 4. Confirmation of cell protection effect against cell damage caused by UVA

In this experimental example, cell survival rate was evaluated to confirm the cell protection effect due to UVA-induced cell damage for Examples 1 to 3.

Specifically, HaCaT cells, which are human skin keratinocytes, were dispensed at 2 Х 10 ⁴ cells/well into a 96 well plate and cultured in an incubator at 37°C and 5% CO ₂ for 24 hours. After 24 hours, all medium was removed, and the serum-free medium was treated with 40 μg/ml of Examples 1 to 3 and Comparative Examples 1 to 3 and cultured for 24 hours. The medium was removed, washed twice with 100 ㎕ of PBS, then treated with 0.25 mg/mL of MTT reagent and incubated for another 2 hours. After dissolving the formed formarzan crystals in DMSO, the cell viability was measured through ELISA measurement at 540 nm, and the cytoprotective effect against UVA-induced cell damage was confirmed by calculating using Equation 4 below. The results are shown in the table below. Shown in 5:

[Equation 4]

Cell viability (%) = ｛1 - OD _sample / OD _control ｝Х 100.

division Cell viability (%) Comparative Example 1 88.8 Comparative Example 2 89.2 Comparative Example 3 87.6 Example 1 114.7 Example 2 106.8 Example 3 108.1

As shown in Table 5, Examples 1 to 3 showed a higher cell protection effect than Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the highest cell protection ability against cell damage caused by UVA among Examples 1 to 3, with a cell viability of about 115%.

Experimental Example 5. Confirmation of whitening effect (Tyrosinase inhibition effect)

In this experiment, in order to confirm the whitening effect of Examples 1 to 3, the tyrosinase inhibition effect was evaluated.

Specifically, 80 μl of 100 mM sodium phosphate buffer (pH 6.8) and 40 μl of Tyrosinase were added to 40 μl of the 200 μg/ml extract and reacted at 37°C for 10 minutes. Afterwards, 40 ㎕ of L-DOPA was added to the reaction solution, reacted at 37°C for 8 minutes, and the tyrosinase inhibitory effect was measured through ELISA measurement at 475 nm. Kojic acid was used as a positive control, and the tyrosinase inhibition effect was calculated using Equation 5 below, and the results are shown in Table 6 and Figure 1:

[Equation 5]

Tyrosinase inhibitory effect (%) = ｛1 - OD _sample / OD _control ｝Х 100.

division Tyrosinase inhibition effect (%) Comparative Example 1 38.4 Comparative Example 2 21.5 Comparative Example 3 22.1 Example 1 60.6 Example 2 37.4 Example 3 48.0

As shown in Table 6 and Figure 1, Examples 1 to 3 showed a higher tyrosinase inhibitory effect than Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the highest whitening effect due to the tyrosinase inhibition effect among Examples 1 to 3, with a tyrosinase inhibition effect of about 61%.

Experimental Example 6. Confirmation of whitening activity (melanin production inhibition effect)

In this experimental example, in order to confirm the whitening effect of Examples 1 to 3, the melanin production inhibition effect was evaluated.

Specifically, B16-F10 cells, which are mouse melanoma cells, were dispensed at a density of 2 Х 10 ⁴ cells/well in a 24 well plate and cultured for 24 hours. Afterwards, to induce melanin production, the cells were treated with new medium containing 100 nM concentration of α-melanocyte stimulating hormone (MSH) and test substances and cultured for 72 hours. Arbutin was used as a control group. After culturing for 72 hours, the culture medium was suctioned, washed with 500 ㎕ of DPBS, and suctioned again. Afterwards, 110 ㎕ of 1 N NaOH (10% DMSO) was added to each well and incubated at 60°C for 1 hour to dissolve. After mixing the culture medium by pipetting, 90 ㎕ was dispensed into another 96 well plate, and the absorbance was measured at 490 nm along with melanin standard material. The amount of melanin produced was calculated using Equation 6 below, and the results of the effect of inhibiting melanin production compared to normal cells are shown in Table 7 and Figure 2:

[Equation 6]

Melanin production inhibition effect (%) = ｛1 - OD _sample / OD _control ｝Х 100.

division Melanin production inhibition effect (%) Comparative Example 1 12.0 Comparative Example 2 15.4 Comparative Example 3 8.0 Example 1 30.0 Example 2 21.3 Example 3 20.6

As shown in Table 7 and Figure 2, Examples 1 to 3 showed a higher melanin production inhibition effect than Comparative Examples 1 to 3, which were each single extract. In addition, Example 1 showed the highest whitening effect due to the melanin production inhibition effect among Examples 1 to 3, with a melanin production inhibition effect of about 30%.

Hereinafter, the present invention will be described in more detail through formulation examples. The formulation examples are only for illustrating the present invention and are not to be construed as limiting the scope of the present invention by the formulation examples.

Formulation example - Preparation of sunscreen containing mixed extracts

A sunscreen containing the mixed extract of Example 1 according to one aspect was prepared by a conventional method according to the components and composition ratios shown in Table 8 below.

ingredient Content (% by weight) mixed extract 0.02 Butylmethoxydibenzoylmethane 2.0 Polysilicon-15 2.0 octocrylene 2.0 Ethylhexyl Salicylate 3.0 Potassium Cetyl Phosphate 3.0 Glyceryl myristate 4.0 cetyl alcohol 2.0 Diisopropyl Sebacate 6.0 Dibutyl adipate 5.0 glycerin 3.0 xanthan gum 0.3 Purified water To 100

The above description is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive.

Claims (20)

A cosmetic composition comprising Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract,
The composition is a cosmetic composition comprising the Camellia sinensis extract, the Camellia sinensis extract, and the Geumbulcho extract in a weight ratio of 2:1:1.
The cosmetic composition according to claim 1, wherein the tea plant is at least one selected from the group consisting of roots, stems, leaves, and flowers of the tea plant.
The cosmetic composition according to claim 1, wherein the plant is at least one selected from the group consisting of roots, stems, leaves, and flowers of the plant.
The cosmetic composition according to claim 1, wherein the Geumbulcho is one or more selected from the group consisting of roots, stems, leaves, and flowers of Geumbulcho.
delete The method of claim 1, wherein the Camellia sinensis extract, the Sorijani extract, or the Geumbulcho extract is extracted with one or more selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol. Phosphorus, cosmetic composition.
delete The cosmetic composition according to claim 1, wherein the composition has one or more activities selected from the group consisting of antioxidant, anti-inflammatory, skin cell protection, and skin whitening.
The cosmetic composition according to claim 8, wherein the antioxidant activity is radical scavenging activity.
The cosmetic composition according to claim 8, wherein the anti-inflammatory agent is one or more selected from the group consisting of NO production inhibition ability and inflammatory cytokine inhibition ability.
The cosmetic composition according to claim 8, wherein the skin cell protecting activity protects skin cells from ultraviolet rays.
The cosmetic composition according to claim 8, wherein the skin whitening activity is at least one selected from the group consisting of tyrosinase activity inhibition ability and melanin production inhibition ability.
A method for producing a cosmetic composition comprising mixing Camellia Sinensis extract, Rumex Crispus extract, and Inula Britannica extract at a weight ratio of 2:1:1.
The method according to claim 13, comprising the steps of ultrasonic extraction of the tea tree to obtain a tea tree extract;
Obtaining a tea tree filtrate by filtering the tea tree extract;
Concentrating the tea tree filtrate to obtain a tea tree concentrate;
Obtaining tea tree extract powder by removing the solvent from the tea tree concentrate; and
A method for producing a cosmetic composition further comprising the step of mixing the tea tree extract powder with a solvent to obtain the tea tree extract.
The method of claim 14, further comprising steaming the tea tree prior to obtaining the tea tree extract.
The method according to claim 13, comprising the steps of ultrasonic extraction of the sorrel to obtain a sortle extract;
Obtaining a sorrel filtrate by filtering the sorrel extract;
Concentrating the filtrate to obtain a filtrate concentrate;
Obtaining a Sorijaeng extract powder by removing the solvent from the Sorijaengi concentrate; and
A method for producing a cosmetic composition further comprising the step of mixing the Sorijaengi extract powder with a solvent to obtain the Sorijagi extract.
The method of claim 16, further comprising the step of steaming the snail before obtaining the extract of the snail.
The method according to claim 13, comprising the steps of ultrasonic extraction of Geumbulcho to obtain Geumbulcho extract;
Obtaining Geumbulcho filtrate by filtering the Geumbulcho extract;
Concentrating the Geumbulcho filtrate to obtain Geumbulcho concentrate;
Obtaining Geumbulcho extract powder by removing the solvent from the Geumbulcho concentrate; and
A method for producing a cosmetic composition further comprising the step of mixing the Geumbulcho extract powder with a solvent to obtain the Geumbulcho extract.
The method of claim 18, further comprising the step of steaming the Geumbulcho extract before the step of obtaining the Geumbulcho extract.
delete