KR20180068524A - SS18-SSX 융합 유전자 특이적 siRNA 및 이를 포함하는 암 예방 또는 치료용 약학적 조성물 - Google Patents
SS18-SSX 융합 유전자 특이적 siRNA 및 이를 포함하는 암 예방 또는 치료용 약학적 조성물 Download PDFInfo
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Abstract
Description
도 2는 259T 환자에서 확인한 SS18-SSX2 융합 유전자와 HS-SY-II 세포주에서 확인한 SS18-SSX1 융합 유전자의 브레이크포인트를 비교한 결과를 나타낸 도이다.
도 3은 설계된 16종의 siRNA를 활막육종 세포주(HS-SY-II)에 형질감염시킨 후 SS18-SSX 융합 유전자 발현의 변화를 qRT-PCR로 확인한 결과를 나타낸 도이다.
도 4는 선별된 3종의 siRNA(siRNA 3, siRNA 6, siRNA 11)를 활막육종 세포주(HS-SY-II)와 유잉육종 세포주(A673)에 형질감염시킨 후 SS18-SSX 융합 유전자 발현의 변화를 qRT-PCR 및 웨스턴 블롯으로 확인한 결과를 나타낸 도이다.
도 5는 3 종의 siRNA(siRNA 3, siRNA 6, siRNA 11)를 활막육종 세포주(HS-SY-II)에 형질감염시킨 후 세포성장억제변화를 WST로 확인한 결과를 나타낸 도이다.
Claims (10)
- 서열번호 1, 서열번호 2 또는 서열번호 3으로 표시되는 센스(sense) 서열 및 이의 안티센스(antisense) 서열을 포함하는, siRNA(small interfering RNA).
- 제1항에 있어서, 상기 siRNA는 SS18(nBAF chromatin remodeling complex subunit)-SSX(SSX family member) 융합 유전자에 특이적으로 결합하는 것인, siRNA.
- 제1항에 있어서, 상기 siRNA가 화학적으로 변형된 것인, siRNA.
- 제3항에 있어서, 상기 화학적 변형은 뉴클레오티드 내 당 구조의 2´ 탄소 위치에서 -OH기가 -CH3, -OCH3, -NH2, -F, -O-2-메톡시에틸 -O-프로필(propyl), -O-2-메틸티오에틸(methylthioethyl), -O-3-아미노프로필, -O-3-디메틸아미노프로필, -O-N-메틸아세트아미도 또는 -O-디메틸아미도옥시에틸로의 치환에 의한 변형; 뉴클레오티드 내 당(sugar) 구조 내의 산소가 황으로 치환된 변형; 뉴클레오티드결합의 포스포로티오에이트(phosphorothioate) 또는 보라노포스페이트(boranophosphate), 메틸포스포네이트(methyl phosphonate) 결합으로의 변형; PNA(peptide nucleic acid), LNA(locked nucleic acid) 또는 UNA(unlocked nucleic acid) 형태로의 변형;으로 구성된 군으로부터 선택된 1종 이상인, siRNA.
- 서열번호 1, 서열번호 2 또는 서열번호 3으로 표시되는 센스(sense) 서열 및 이의 안티센스(antisense) 서열을 포함하는, 암 예방 또는 치료용 약학적 조성물.
- 제5항에 있어서, 상기 암은 SS18-SSX 융합 유전자 양성 암인, 암 예방 또는 치료용 약학적 조성물.
- 제5항에 있어서, 상기 암은 활막육종인, 암 예방 또는 치료용 약학적 조성물.
- 제5항에 있어서, 핵산 전달체를 더 포함하는, 암 예방 또는 치료용 약학적 조성물.
- 서열번호 1, 서열번호 2 또는 서열번호 3으로 표시되는 센스(sense) 서열과 이의 안티센스(antisense) 서열을 포함하는 siRNA 및 핵산 전달체를 포함하는, siRNA 복합체.
- 제9항에 있어서, 상기 핵산 전달체는 바이러스성 벡터, 비바이러스성 벡터, 리포좀, 양이온성 고분자, 미셀(micelle), 에멀젼 및 지질 나노입자(solid lipid nanoparticles)로 구성된 군에서 선택되는 것인, siRNA 복합체.
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KR20210042292A (ko) | 2019-02-26 | 2021-04-19 | 서울대학교산학협력단 | Tut4/7 발현 조절인자를 포함하는 암 예방 또는 치료용 약학적 조성물 |
KR20210042290A (ko) | 2019-02-26 | 2021-04-19 | 서울대학교산학협력단 | Tut4/7 발현 조절인자를 포함하는 암 예방 또는 치료용 약학적 조성물 |
KR20220124112A (ko) | 2019-02-26 | 2022-09-13 | 서울대학교산학협력단 | Tut4/7 발현 조절인자를 포함하는 암 예방 또는 치료용 약학적 조성물 |
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