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KR20170044194A - 아데노바이러스의 제조를 위한 공정 - Google Patents

아데노바이러스의 제조를 위한 공정 Download PDF

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KR20170044194A
KR20170044194A KR1020177008230A KR20177008230A KR20170044194A KR 20170044194 A KR20170044194 A KR 20170044194A KR 1020177008230 A KR1020177008230 A KR 1020177008230A KR 20177008230 A KR20177008230 A KR 20177008230A KR 20170044194 A KR20170044194 A KR 20170044194A
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브라이언 로버트 챔피언
지텐드라 바티아
애쉬빈 파텔
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싸이오서스 테라퓨틱스 엘티디.
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Abstract

아데노바이러스의 제조를 위한 공정. 본 개시내용은 아데노바이러스의 제조를 위한 연속 공정에 관한 것이고, 상기 공정은, A) 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 상기 세포는 바이러스 복제를 지지할 수 있음), 및 B) 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하되, 바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지되고, 상기 공정은 적어도 하나의 배지 변경 또는 첨가 및 적어도 하나의 세포 변경 또는 첨가를 포함한다. 본 개시내용은 또한 상기 방법으로부터 얻어지거나 얻어질 수 있는 바이러스 집단으로 연장된다.

Description

아데노바이러스의 제조를 위한 공정{A PROCESS FOR THE PPODUCTION OF ADENOVIRUS}
본 개시내용은 소정의 아데노바이러스, 예를 들어 키메라 아데노바이러스 및/또는 복제 가능 아데노바이러스, 및/또는 B 그룹 바이러스의 제조 방법 및 이들로부터 얻어진 바이러스 생성물에 관한 것이다.
본 명세서는 2014년 8월 27일에 출원된 GB1415154.2 및 2014년 9월 3일에 출원된 GB1415581.6(이들 둘 다 본 명세서에 참고로 포함됨)에 대한 우선권을 주장한다.
현재, 약제학적 분야는 인간 사용을 위한 치료제로서 바이러스의 가능성을 막 실현하려는 참이다. 현재까지, ONXY-15(ONYX Pharmaceuticals 및 Shanghai Sunway Biotech)에 의해 획득)로부터 유래한 바이러스는 제한된 수의 나라에서 두경부암에 대해 사용에 대해 승인받았다. 그러나, 현재 임상에서 다수의 바이러스가 존재하고, 이들은 이들 중 일부가 희망적으로 인간에서 사용하기 위해 등록되게 해야 한다.
다수의 바이러스 치료제는 아데노바이러스에 기초하고, 예를 들어 EnAd(ColoAd1)는 현재 결장암의 치료에 대한 임상 실험에 있는 키메라 종양 아데노바이러스(WO2005/118825)이다.
이들 아데노바이러스 기반 치료제는 등록 후 임상 실험 및 수요 둘 다를 지지하기에 적합한 분량으로 및 우수의약품 제조관리 기준(good manufacturing practice; GMP)을 고수하는 조건 하에 제조될 필요가 있다.
제조 공정의 일부로서, 바이러스는 시험관내, 예를 들어 세포 현탁 배양에서 포유류 세포에서 전파된다. 바이러스는 세포 용해 및 후속하는 정제에 의해 이들 세포로부터 회수된다. 도 1은 카멘(Kamen) 및 헨리(Henry)(2004)(J Gene Med. 6: 184-192 페이지)로부터의 추출물이고, GMP 등급 아데노바이러스의 공정 관여 제조의 도식적 다이어그램을 보여준다. 특히, 바이러스 복제 후, 세포가 용해된다.
용해 후 세포로부터 DNA 및 숙주 세포 단백질(host cell protein; HCP)을 오염시키는 것은 상당한 문제일 수 있고, 최종 치료학적 아데노바이러스 생성물로부터 가능한 멀리 제거되어야 한다. 이것은 출원 WO2011/045381(세포 현탁액 내에서 세포를 용해시키거나, DNA를 단편화하거나, 침전시키고, 접선 흐름을 이용하여 이를 청징하는 것을 기재함)에 자세히 기재되어 있다. DNAse에 의한 DNA 분해는 도 1에서 제3 단계로서 또한 도시되어 있다.
성공적인 재조합 아데노바이러스 공정의 개발은 기본적인 숙주 세포주 생리학 및 대사; 재조합 바이러스, 및 세포주와 바이러스 사이의 상호작용의 상세한 이해를 요한다. 본질적으로, 상기 공정은 특정한 유형의 바이러스 또는 바이러스 벡터에 따라 적응을 요한다.
또한, 규제 허가를 얻은 재조합 바이러스 생성물에 대해 임상 수요가 충족될 수 있게 보장하도록 그리고 제조 비용을 감소시키기 위해 임상 사용에 적합한 재조합 바이러스를 제조하는 비용은 비교적 높다. 제조의 효율을 증가시키는, 예를 들어 바이러스의 수율을 증가시키는 개선된 공정이 필요하다.
놀랍게도, 본 발명자들은 세포 배지로부터 바이러스를 단리시키고, 세포를 용해시키기 위한 필요성을 없애고, 따라서 바이러스 생성물에서 DNA 및 HCP 오염의 시작 수준을 상당히 감소시킬 수 있는 연속 공정에 의해 소정의 아데노바이러스, 예를 들어 복제 가능(replication competent) 아데노바이러스 및 키메라 종양 아데노바이러스, 및 B 그룹 아데노바이러스가 제조될 수 있다는 것을 확립하였다.
또한, 본 발명자들은 바이러스, 예를 들어 B 그룹 바이러스 생성물이 소정의 시점에서 배양물에, 즉 상청액에 또는 세포 펠렛과 연관되어 위치하는 곳에 대한 제어를 제공하는 매개변수를 확립하였다. 이것은 공정이 필요한 바대로 적응되게 한다.
따라서, 본 개시내용은 아데노바이러스(예를 들어, B 그룹 아데노바이러스)의 제조를 위한 연속 공정으로서, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
b. 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지된다.
따라서, 본 개시내용은 복제 가능 아데노바이러스; B 그룹 바이러스, 아데노바이러스 사멸 단백질을 암호화하지 않거나 발현하지 않는 아데노바이러스, E2B 영역을 포함하는 게놈을 가지는 복제 가능 또는 결핍 키메라 종양 아데노바이러스로 이루어진 군으로부터 선택된 바이러스의 제조를 위한 연속 공정(상기 E2B 영역은 제1 아데노바이러스 혈청형으로부터 유래한 핵산 서열 및 제2의 구별되는 아데노바이러스 혈청형으로부터 유래한 핵산 서열을 포함하고; 상기 제1 혈청형 및 제2 혈청형은 각각 아데노바이러스 B, C, D, E 또는 F 하위군, 및 상기의 2개 이상의 조합으로부터 선택됨)을 제공하고, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
b. 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지된다.
따라서, 일 실시형태에서, 본 개시내용은
복제 가능 아데노바이러스; 또는
E2B 영역을 포함하는 게놈을 가지는 복제 가능 또는 결핍 키메라 종양 아데노바이러스(여기서, 상기 E2B 영역은 제1 아데노바이러스 혈청형으로부터 유래한 핵산 서열 및 제2의 구별되는 아데노바이러스 혈청형으로부터 유래한 핵산 서열을 포함하고; 상기 제1 혈청형 및 제2 혈청형은 아데노바이러스 B, C, D, E 또는 F 하위군으로부터 각각 선택됨)의 제조를 위한 연속 공정으로서, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
b. 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지된다.
독립적인 일 양태에서, B형 아데노바이러스의 제조를 위한 연속 공정이 제공되고, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
b. 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지된다.
일 실시형태에서, B형 아데노바이러스는 E2B 영역을 포함하는 게놈을 가지는 키메라 종양 아데노바이러스이고, 상기 E2B 영역은 제1 아데노바이러스 혈청형으로부터 유래한 핵산 서열 및 제2의 구별되는 아데노바이러스 혈청형으로부터 유래한 핵산 서열을 포함하고; 상기 제1 혈청형 및 제2 혈청형은 각각 아데노바이러스 B, C, D, E 또는 F 하위군으로부터 선택된다.
일 실시형태에서, 종양 바이러스는
동일한 혈청형으로부터의 섬유, 헥손 및 펜톤 단백질, 예를 들어 Ad11, 특히 Ad11p(예를 들어, 후자의 게놈 서열의 30812-31789, 18254-21100 및 13682-15367의 위치에서 발견됨)를 가지고, 뉴클레오타이드 위치는 유전자은행 217307399호(수탁 번호: GC689208)에 대한 것이다.
일 실시형태에서, 아데노바이러스는 에나데노투시레브(enadenotucirev)(또한 EnAd로 그리고 이전에 EnAd로 공지됨)이다. 본 명세서에 사용된 바와 같은, 에나데노투시레브는 서열 번호 12의 키메라 아데노바이러스를 의미한다. 이것은 야생형 아데노바이러스와 비교하여 증대된 치료학적 특성을 가지는 복제 가능 종양 키메라 아데노바이러스이다(WO2005/118825 참조). EnAd는 Ad11p 및 Ad3으로부터의 DNA, 및 E3/E4에서의 결실을 특징으로 하는 키메라 E2B 영역을 가진다. 에나데노투시레브의 구조 변경은 Ad11p보다 대략 3.5kb 작은 게놈을 생성시켜서 전이유전자의 삽입에 대한 추가의 "공간"을 제공한다.
OvAd1 및 OvAd2는 또한 게놈에서 추가의 "공간"을 또한 가지는 에나데노투시레브와 유사한 키메라 아데노바이러스이다(WO2008/080003 참조). 따라서, 일 실시형태에서, 아데노바이러스는 OvAd1 또는 OvAd2이다.
일 실시형태에서, B 그룹 아데노바이러스는 하기 화학식 (I)을 포함하는 게놈을 포함한다:
Figure pct00001
식 중,
B1은 결합이거나, E1A, E1B 또는 E1A-E1B를 포함하고;
BA는 E2B-L1-L2-L3-E2A-L4이고;
B2는 결합이거나, E3을 포함하고;
BX는 결합 또는 제한 부위, 하나 이상의 전이유전자 또는 둘 다를 포함하는 DNA 서열이고;
BB는 L5를 포함하고;
BY는 결합 또는 제한 부위, 하나 이상의 전이유전자 또는 둘 다를 포함하는 DNA 서열이고;
B3은 결합이거나, E4를 포함하고;
BX 및 BY 중 적어도 하나는 결합이 아니고, 예를 들어 BX 및 BY 중 적어도 하나는 전이유전자, 제한 부위 또는 둘 다, 예컨대 전이유전자를 포함한다.
따라서, 일 실시형태에서, 본 개시내용에 따른 연속 공정이 제공되고, B형 아데노바이러스는 복제 가능하다.
화학식 (I)의 서열을 포함하는 복제 가능 B 그룹 아데노바이러스:
Figure pct00002
식 중,
B1은 결합이거나, E1A, E1B 또는 E1A-E1B를 포함하고;
BA는 -E2B-L1-L2-L3-E2A-L4를 포함하고;
B2는 결합이거나, E3을 포함하고;
BX는 결합 또는 제한 부위, 하나 이상의 전이유전자 또는 둘 다를 포함하는 DNA 서열이고;
BB는 L5를 포함하고;
BY는 전이유전자 및 스플라이스 억셉터 서열을 포함하는 전이유전자 카세트를 포함하고;
B3은 결합이거나, E4를 포함하고,
전이유전자 카세트는 E4 및 주요 후기 프로모터로 이루어진 군으로부터 선택된 내인성 프로모터의 제어 하에 있고, 전이유전자 카세트는 편향되지 않게 삽입하는 전이인자(transposon)를 포함하지 않는다.
화학식 (I)의 바이러스는 본 명세서에 참고로 포함된 WO2015/059303에 개시되어 있다.
일 실시형태에서, 아데노바이러스는 서열 목록에서 본 명세서에 개시된 서열이다.
일 실시형태에서, 본 개시내용의 공정에서 사용된 바이러스는 전장 미만의 아데노바이러스 게놈을 함유하고, 예를 들어 아데노바이러스 게놈 또는 엄격한 조건 하에 아데노바이러스 게놈의 50%, 60%, 70%, 80% 이상에 혼성화하는 서열의 50%, 60%, 70%, 80% 이상을 함유한다.
일 실시형태에서, 본 개시내용의 바이러스는 결실된 E3 영역의 일부 또는 전부를 가진다. 이론에 구속되고자 바라지 않으면서, 이 영역의 부분 또는 완전 결실이 바이러스 복제의 속도를 증가시킬 수 있고, 이것이 몇몇 경우에 생체내 유리한 특성을 제공할 수 있다는 것이 본 발명자들에 의해 생각되었다.
일 실시형태에서, 본 개시내용에 사용된 바이러스는 결실된 E4 영역의 일부 또는 전부를 가진다. 이론에 구속되고자 바라지 않으면서, E4 영역의 부분 결실이 바이러스 복제의 속도를 증가시킬 수 있고, 이것이 몇몇 경우에 생체내 유리한 특성을 제공할 수 있다는 것이 본 발명자들에 의해 생각되었다.
일 실시형태에서, 본 개시내용에 사용된 바이러스는 바이러스가 이의 복제 역량을 보유하도록 E4 영역에서 부분적으로 결실된다.
일 실시형태에서, 본 개시내용에 사용된 바이러스는, 적절한 바대로, 결실된 E3 영역의 일부 또는 전부 및 결실된 E4 영역의 일부 또는 전부를 가진다.
일 실시형태에서, 아데노바이러스는 B 그룹 아데노바이러스, 예를 들어 Ad11 또는 EnAd로부터 헥손 및 섬유를 가진다.
일 실시형태에서, 예를 들어 B 하위군의 섬유 및 헥손을 가지는 아데노바이러스(예컨대, Ad11, 특히 슬로비트스키(Slobitski) 균주로 또한 공지된 Ad11p)의 제조를 위한 연속 공정(여기서, E3의 일부 또는 전부 및/또는 모든 E4 영역의 일부가 결실됨)이 제공되고, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
b. 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지된다.
일 실시형태에서, 본 개시내용에서 사용된 바이러스는 전이유전자를 포함할 수 있다.
일 실시형태에서, 본 개시내용의 아데노바이러스, 예컨대 B형 아데노바이러스는 복제 가능 또는 결핍이다.
일 실시형태에서, 아데노바이러스는 복제 가능 또는 복제 결핍, 예를 들어 복제 가능이다. 복제 결핍 아데노바이러스는 또한 본 명세서에서 바이러스 벡터라 칭해진다.
일 실시형태에서, 키메라 바이러스는 복제 가능 또는 복제 결핍, 예를 들어 복제 가능이다.
일 실시형태에서, 본 개시내용에 사용된 바이러스는 기능적 아데노바이러스 사멸 단백질 또는 이의 기능적 단편을 발현하지 않고, 특히 아데노바이러스 사멸 단백질 또는 이의 단편을 발현하지 않는다.
일 실시형태에서, 본 개시내용의 바이러스는 기능적 아데노바이러스 사멸 단백질 또는 이의 기능적 단편을 암호화하는 DNA 서열을 포함하지 않고, 특히 아데노바이러스 사멸 단백질 또는 이의 단편을 암호화하는 서열을 포함하지 않는다.
일 실시형태에서, 본 개시내용에 사용된 아데노바이러스는 콕사키바이러스 및 아데노바이러스 수용체(coxsackievirus and adenovirus receptor; CAR)를 통해 세포를 감염시키는 것이 아니다.
일 실시형태에서, 본 개시내용에 사용된 아데노바이러스는 CD46, 예를 들어 B 그룹 아데노바이러스를 통해 세포를 감염시키는 것이다.
일 실시형태에서, 바이러스, 예를 들어 복제 가능 바이러스는 C 그룹 바이러스가 아니다.
일 실시형태에서, 바이러스, 예를 들어 복제 가능 바이러스는 Ad5가 아니다.
일 실시형태에서, 연속 제조 기간은 적어도 2회의 바이러스 주기, 예를 들어 70시간 내지 300시간이다.
일 실시형태에서, 상기 공정은 적어도 2개의 수확 단계를 포함한다.
일 실시형태에서, 상기 공정은, 감염 후 적어도 24시간, 예컨대 30시간 또는 35시간에 개시되고, 예를 들어 공정의 종료까지 지속하는, 바이러스의 연속 수확을 포함한다.
일 실시형태에서, 예를 들어 상기 공정의 종료 시, 특히 세포에 보유된 바이러스를 회수하기 위해 단일 세포 용해 단계가 존재한다.
일 실시형태에서, 상기 공정은 수확된 하나 이상의 분획 바이러스를 조합하는 단계를 포함한다.
일 실시형태에서, 상기 공정은, 예를 들어 일 이상의 시점, 예컨대 24, 30, 35, 40, 45, 48, 50, 55, 60, 65, 70, 72, 75, 80, 85, 90, 95 또는 96시간에서, 독립적으로 선택된 일 이상의 경우에, 예를 들어 1개, 2개, 3개, 4개 또는 5개의 첨가에서 배양물에 새로운 세포를 첨가하는 단계를 포함한다.
일 실시형태에서, 상기 공정은 새로운 배지가 첨가되는 적어도 1개의 단계를 포함한다. 일 실시형태에서, 상기 공정은 예를 들어 감염 후 12시간 내지 96시간, 예컨대 24, 30, 35, 40, 45, 48, 50, 55, 60, 65, 70, 72, 75, 80, 85, 90, 95 또는 96시간의 임의의 시점에서 적어도 하나의 배지 변경 또는 첨가를 포함한다.
일 실시형태에서, 상기 공정은
예를 들어 일 이상의 시점, 예컨대 24, 30, 35, 40, 45, 48, 50, 55, 60, 65, 70, 72, 75, 80, 85, 90, 95 또는 96시간에서, 독립적으로 선택된 일 이상의 경우에, 예를 들어 1개, 2개, 3개, 4개 또는 5개의 첨가에서 배양물에 새로운 세포를 첨가하는 단계, 및
예를 들어 감염 후 12시간 내지 96시간, 예컨대 24, 30, 35, 40, 45, 48, 50, 55, 60, 65, 70, 72, 75, 80, 85, 90, 95 또는 96시간의 임의의 시점에서 새로운 배지의 첨가 또는 배지 예의 변경의 단계를 포함한다.
일 실시형태에서, 세포는 1-91 내지 9×104vp/㎖ 이상, 예컨대 1 내지 9×105, 1 내지 9×106, 1 내지 9×107, 1 내지 9×108, 1 내지 9×109vp/㎖, 특히 1 내지 5×107vp/㎖, 예컨대 약 1×106, 4 내지 5×106, 특히 1×106vp/㎖의 바이러스의 시작 농도에 의해 감염된다.
일 실시형태에서, 감염 다중도((multiplicity of infection; MOI)는 2 내지 75의 범위, 예를 들어 5, 6, 7, 8, 9, 10, 11, 12, 12.5, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 31.25, 32, 33, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55 등, 예컨대 12.5, 31.25 또는 50이다. 일 실시형태에서, 감염 다중도는 10 내지 15의 범위, 예컨대 12.5vp/세포이다.
일 실시형태에서, MOI는 2 내지 50, 예를 들어 5 내지 20, 예컨대 6, 7, 8, 9, 10, 11, 12, 13, 14 또는 15의 범위, 예컨대 12.5vp/세포이다.
일 실시형태에서, 시드 밀도는 1.9×106 또는 4×106이고, 감염 다중도는 50이다.
일 실시형태에서, 시드 밀도는 1 내지 1.9×106의 범위, 예컨대 1×106vp/㎖이고, 감염 다중도는 10 내지 15의 범위, 예컨대 12.5이다.
본 개시내용의 일 실시형태에서, 사용된 배양은 관류 배양이다.
본 개시내용의 일 실시형태에서, 사용된 세포는 부착성이다.
본 개시내용의 일 실시형태에서, 사용된 세포는 현탁 배양에 적합하다.
일 실시형태에서, 본 개시내용의 공정에서 사용된 배양은 현탁 배양, 부착성 배양, 관류 배양 또는 이들의 조합, 예컨대 현탁 배양이다.
본 개시내용에 따른 연속 제조 공정은 이것이 하나 이상의 하기 이점을 가진다는 점에서 유리하다: 바이러스의 적절한 분량이 임상 사용을 위해 준비되게 함으로써 제조의 효율을 증가시키는 것, 제조 캠페인에서 소모된 시간을 감소시키는 것(결국 상품 비용을 감소시킴), 및 또한 이것이 복수의 마스터 바이러스 시드 스톡을 제조할 필요를 최소화시킨다(비용을 또한 감소시킬 수 있음)는 점에서 제조의 복잡함을 감소시키는 것.
본 명세서에 기재된 공정의 다른 이점은 상기 공정의 규모가 예를 들어 증가한 수율로 인해 감소되어야 하는 능력을 포함한다.
더욱이, 본 발명자들은 세포가 세포마다 높은 수준의 바이러스를 제조하게 하는 매개변수를 확립하였다. 이 독립적인 양태에서, 배양은 예를 들어 낮은 시작 시드 세포 밀도와 조합된 낮은 감염 다중도를 가진다는 것을 특징으로 한다.
따라서, 독립적인 일 양태에서, 아데노바이러스를 복제하기에 적합한 세포를 감염시키는 방법이 제공되고, 시작 시드 밀도는 2×106vp/㎖ 이하, 예를 들어 1.5×106vp/㎖ 이하, 예컨대 1×106vp/㎖이고, 감염 다중도는 15 이하, 예컨대 14, 13, 12.5, 12, 11 또는 10, 예컨대 12.5이다.
따라서, 독립적인 일 양태에서, 아데노바이러스를 복제하기에 적합한 세포를 감염시키는 방법이 제공되고, 시작 시드 밀도는 1.9×106이고, 감염 다중도는 50ppc이다.
소정의 시점, 예컨대 약 72시간 이상에서 세포마다 200,000개만큼의 높은 바이러스 입자의 수율이 본 명세서에 기재된 조건을 이용하여 달성될 수 있다. 일 실시형태에서, 예를 들어 48, 50, 55, 60, 65 또는 70시간 후 100,000개 초과로 세포마다 바이러스가 제조된다.
배양물이 낮은 시드 밀도 및 낮은 감염 다중도를 이용하는 이 실시형태에서, 바이러스가 용이하게 수확될 수 있다.
또한, 본 발명자들은 공정의 매개변수를 변경함으로써 바이러스가 예를 들어 세포, 상청액 또는 이들의 조합에서 위치하는 곳에 대해 제어가 제공될 수 있다는 것을 확립하였다. 이것은 본 개시내용의 추가의 양태를 형성한다.
특히 낮은 감염 다중도와 함께 낮은 시드 밀도는 세포에 주로 위치한 바이러스 생성물을 생성시키지만, 공정의 종료에 향하면서 바이러스가 상청액에서 또한 발견된다. 이것은 특히 배지가 변경될 때 세포마다 매우 높은 수율을 또한 제공하였다.
높은 감염 다중도 및 높은 시드 밀도는 또한 공정의 종료에 향하면서 상청액에서 대부분의 바이러스 입자를 생성시킨다.
높은 감염 다중도는 낮은 시드 밀도와 조합되어 공정의 종료에 향하면서 상청액에서 대부분의 바이러스 생성물을 생성시킨다.
반대로, 낮은 감염 다중도는 높은 시드 밀도와 함께 특히 배지가 변경되지 않을 때 세포에서 바이러스를 제공하였다.
보통의 감염 다중도는 보통의 시드 밀도와 조합되어 특히 배지 변경이 없을 때 상청액 및 세포에서 바이러스 생성물을 제공한다.
더욱이, 높은 감염 다중도 및 높은 시드 밀도는 배지의 변경에 의해 또는 변경 없이 주로 상청액에서 바이러스 생성물을 제공하는 것으로 보인다.
따라서, 놀랍게도, 공정의 매개변수를 제어함으로써, 바이러스 생성물의 위치가 제어될 수 있다. 일 실시형태에서, 상청액과 숙주 세포 사이에 재조합 바이러스(특히 본 명세서에 기재된 아데노바이러스)의 분배를 제어하는 방법이 제공되고, 상기 방법은 a) 숙주 세포 배양(특히 현탁 배양으로서 특히 포유류 세포, 예컨대 본 명세서에 기재된 것)을 제공하는 것, b) 상청액 및 숙주 세포로부터 선택된 원하는 위치에서 바이러스 생성물을 제공하기 위해 시드 밀도, 감염 다중도 및 감염의 기간(배양의 기간)을 선택하는 것, c) 관련 시점에서 배양 상청액 및 숙주 세포에서 재조합 바이러스의 수율을 결정하는 것 및 d) 단계 b)에서 선택된 시드 밀도 및 감염 다중도를 유지시키도록 선출하는 단계(c)에서 결정된 상청액 및 세포의 수율을 비교하는 것 또는 재조합 바이러스의 주요 회수에 맞도록 상청액과 세포 사이에 재조합 바이러스의 분배를 변경하도록 시드 밀도, 감염 다중도 또는 둘 다를 변경하는 것을 포함한다.
일 실시형태에서, 본 개시내용에 따른 매개변수를 조절하는 공정은 상이한 조건에 평행하게 수행된 단계, 즉 시드 밀도, 감염 다중도 또는 둘 다가 제1 공정에서 이용된 매개변수로부터 변경된 공정을 포함하여서, 2개의 상이한 공정의 비교를 허용한다.
일 실시형태에서, 재조합 바이러스의 80% 이상은 상청액에 있고, 예를 들어 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 또는 100%는 상청액에 위치한다.
일 실시형태에서, 48-65시간의 감염 기간에 걸쳐, 예를 들어 본 명세서에 정의된 바대로 낮은 감염 다중도가 사용되고 낮은 시드 밀도가 사용될 때, 재조합 바이러스의 80% 이상은 세포에 있고, 예를 들어 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 또는 100%는 세포에 위치한다. 배지 변경 또는 첨가는 수율을 최대화하도록 또한 도울 수 있다.
따라서, 독립적인 일 양태에서, 아데노바이러스(예를 들어, 본 명세서에 기재된 바이러스, 예컨대 B 그룹 아데노바이러스)의 제조를 위한 공정이 제공되고, 상기 공정은
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 바이러스의 시작 시드 밀도는 1 내지 2×106vp/㎖(예컨대, 1×106vp/㎖)의 범위이고, 감염 다중도는 5 내지 20, 예컨대 10 내지 15의 범위, 특히 12.5임), 및
b. 예를 들어 용해 단계가 감염 후 65시간 내지 70시간에, 예컨대 감염 후 66시간, 67시간, 68시간 또는 69시간에 수행되는 경우 세포로부터 바이러스를 수확하기 위해 바이러스 감염 후 24시간 내지 75시간의 기간에 용해 단계를 수행하는 단계를 포함한다.
일 실시형태에서, 상기 공정은 GMP 제조 공정이다.
본 개시내용의 바이러스는 문맥이 달리 표시하지 않은 한 키메라 종양 아데노바이러스를 포함하는 복제할 수 있는, 복제 가능 또는 복제 결핍 아데노바이러스를 일반적으로 의미하도록 본 명세서에 일반적으로 사용된다.
본 명세서에 사용된 바와 같은, 복제 가능은 세포에서 선택적으로 복제할 수 있는 복제 가능 바이러스 또는 바이러스들을 의미한다. 암 세포에서 선택적으로 복제하는 바이러스는 복제하는 암 세포에서 상향조절되는 유전자 또는 단백질, 예컨대 p53 유전자를 요하는 것이다.
본 명세서에 사용된 바와 같은, 복제 가능 바이러스는 필수 바이러스 단백질을 암호화하는 상보성 세포주, 예컨대 E1 영역에 의해 암호화된 것(또한 패키징 세포주라 칭해짐)의 도움 없이 복제할 수 있는 바이러스 및 헬퍼 바이러스의 도움 없이 복제할 수 있는 바이러스를 의미한다.
일 실시형태에서, 바이러스는 복제 가능하다.
복제 결핍 바이러스는 벡터이고, 복제할 수 있는 패키징 세포주 또는 헬퍼 바이러스의 사용을 요한다.
이용되는 아데노바이러스는, 문맥이 달리 표시하지 않은 한, 일반적으로 복제 가능 아데노바이러스 또는 복제 결핍, 예를 들어 B 그룹 바이러스, 특히 Ad11, 예컨대 Ad11p라 칭해질 것이다. 몇몇 경우에, 이것은 오직 복제 가능 바이러스를 언급하도록 이용될 것이고, 이것은 문맥으로부터 명확할 것이다.
일 실시형태에서, 아데노바이러스는 복제 가능하다.
아데노바이러스 벡터는 일반적으로 복제 결핍 아데노바이러스를 의미할 것이다.
본 명세서에 사용된 바와 같은, B 하위군(B 그룹 또는 B형)은 B 그룹 아데노바이러스로부터 적어도 섬유 및 헥손, 예를 들어 B 그룹 바이러스로부터 전체 캡시드, 예컨대 B 그룹 바이러스로부터 실질적으로 전체 게놈을 가지는 바이러스를 의미한다. 일 실시형태에서, B 그룹 바이러스는 아데노바이러스 사멸 단백질, 예를 들어 11.64K달톤의 ADP 단백질, 예컨대 유니프로트(Uniprot) 번호 P24935를 가지는 단백질을 암호화하지 않는다.
현재까지 시험된 모든 인간 아데노바이러스 게놈은 동일한 일반 체계화를 가지고, 즉 특이적 기능을 암호화하는 유전자는 바이러스 게놈에서 동일한 위치(본 명세서에서 구조 구성요소라 칭해짐)에 위치한다. 바이러스 게놈의 각각의 말단은 바이러스 복제에 필요한 역전 말단 반복부(또는 ITR(inverted terminal repeat))로 공지된 짧은 서열을 가진다. 바이러스 게놈은 5개의 조기 전사 단위(E1A, E1B, E2, E3, 및 E4), 3개의 지연 조기 단위(IX, IVa2 및 E2 후기) 및 1개의 후기 단위(주요 후기)(후기 mRNA의 5개의 패밀리(L1-L5)를 생성시키도록 처리됨)를 함유한다. 조기 유전자에 의해 암호화된 단백질은 주로 감염에 대한 숙주 세포 반응의 복제 및 조절에 관여하지만, 후기 유전자는 바이러스 구조 단백질을 암호화한다. 조기 유전자는 철자 E가 앞에 붙고, 후기 유전자는 철자 L이 앞에 붙는다.
아데노바이러스의 게놈은 단단히 충전되고, 즉 비암호화 서열이 아주 적고, 따라서 전이유전자를 삽입하기 위해 적합한 위치를 찾는 것이 어려울 수 있다. 본 발명자들 전이유전자가 관용성인, 특히 확인된 부위가 복잡한 전이유전자를 수용하기에 적합한 2개의 DNA 영역, 예컨대 항체를 암호화하는 것을 확인하였다. 즉, 전이유전자, 즉 화학식 (I)의 바이러스에서 위치 BX 및/또는 위치 BY는 바이러스의 생존능력, 네이티브 특성, 예컨대 종양 특성 또는 복제에 부정적으로 영향을 미치지 않으면서 발현된다.
일 실시형태에서, 아데노바이러스는 E3 영역에서 부분적으로 또는 완전히 결실된다.
본 명세서에 사용된 바와 같은, E3 영역의 일부가 결실된다는 것은 E3 영역의 예를 들어 1% 내지 99%의 범위의 적어도 일부가 결실된다, 예컨대 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94 95, 96, 97 또는 98%가 결실된다는 것을 의미한다.
일 실시형태에서, 아데노바이러스는 E4 영역에서 부분적으로 결실된다. 바이러스는 E4 영역의 오직 일부가 결실될 때 복제 가능으로 유지될 수 있다.
본 명세서에 사용된 바와 같은, E4 영역의 일부가 결실된다는 것은 E4 영역의 예를 들어 1% 내지 99%의 범위의 적어도 일부가 결실된다, 예컨대 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94 95, 96, 97 또는 98%가 결실된다는 것을 의미한다. 본 개시내용의 아데노바이러스에서, 충분한 E4는 복제를 허용하도록 보유되어야 한다.
본 명세서에 사용된 바와 같은, 키메라(또는 키메라 바이러스)는 일반적으로 적어도 2개의 상이한 혈청형, 예를 들어 B, C, D, E 및 F 그룹, 예컨대 복제할 수 있는, 복제 가능 또는 복제 결핍 키메라 종양 바이러스로부터 독립적으로 혈청형으로부터 게놈 DNA를 포함하는 아데노바이러스를 의미할 것이다. 일 실시형태에서, 키메라 종양 아데노바이러스는 복제 가능, 예를 들어 EnAd, OvAd1 또는 OvAd2이다.
일 실시형태에서, 키메라 바이러스는 EnAd(서열 번호 12) 또는 이의 유도체, 예를 들어 전이유전자 또는 전이유전자들(이의 예는 하기 기재됨)을 혼입하도록 채택된 유도체이다.
일 실시형태에서, 키메라는 E3 영역에서 부분적으로 또는 완전히 결실된다.
본 명세서에 사용된 바와 같은, E3 영역의 일부가 결실된다는 것은 E3 영역의 예를 들어 1% 내지 99%의 범위의 적어도 일부가 결실된다, 예컨대 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94 95, 96, 97 또는 98%가 결실된다는 것을 의미한다.
일 실시형태에서, 키메라는 E4 영역에서 부분적으로 또는 완전히 결실된다.
본 명세서에 사용된 바와 같은, E4 영역의 일부가 결실된다는 것은 E4 영역의 예를 들어 1% 내지 99%의 범위의 적어도 일부가 결실된다, 예컨대 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94 95, 96, 97 또는 98%가 결실된다는 것을 의미한다. 본 개시내용의 아데노바이러스에서, 충분한 E4는 복제를 허용하도록 보유되어야 한다.
본 명세서에 사용된 바와 같은, 전이유전자는 바이러스에 천연이 아니거나(외인성), 바이러스에서 이 특정한 위치에서 보통 발견되지 않는 유전자인 게놈 서열로 삽입된 유전자를 의미한다. 전이유전자의 예는 하기 제공된다. 본 명세서에 사용된 바와 같은, 전이유전자는 또한 삽입될 때 전장 유전자의 기능 또는 대부분의 기능을 수행하기에 적합한 유전자의 부분인 유전자의 기능적 단편을 포함한다.
전이유전자 및 암호화 서열은, 문맥이 달리 표시하지 않은 한, 바이러스 게놈으로의 삽입체의 맥락에서 본 명세서에서 상호 교환되어 사용된다. 본 명세서에 사용된 바와 같은, 암호화 서열은 예를 들어 기능적 RNA, 펩타이드, 폴리펩타이드 또는 단백질을 암호화하는 DNA 서열을 의미한다. 통상적으로, 암호화 서열은 관심 있는 기능적 RNA, 펩타이드, 폴리펩타이드 또는 단백질을 암호화하는 전이유전자에 대한 cDNA이다. 관심 있는 기능적 RNA, 펩타이드, 폴리펩타이드 및 단백질은 하기 기재되어 있다.
명확하게, 바이러스 게놈은 DNA의 암호화 서열을 함유한다. 바이러스의 게놈 서열에서 내인성(천연 발생 유전자)은, 이후 재조합 기법에 의해 변형되지 않는 한, 예컨대 이들이 비천연 위치 또는 비천연 환경에 있거나, 이들이 비천연 기능을 가지는 않는 한, 본 명세서의 맥락 내에, 전이유전자로 생각되지 않는다.
일 실시형태에서, 본 명세서에 사용된 바와 같은, 전이유전자는 하나의 유기체로부터 단리되고 상이한 유기체, 즉 본 개시내용의 바이러스로 도입된 유전자 또는 cDNA 서열을 함유하는 DNA의 분절을 의미한다. 일 실시형태에서, DNA의 이 비네이티브 분절은 기능적 RNA, 펩타이드, 폴리펩타이드 또는 단백질을 생성하는 능력을 보유할 수 있다.
따라서, 일 실시형태에서, 삽입된 전이유전자는 인간 또는 인간화 단백질, 폴리펩타이드 또는 펩타이드를 암호화한다.
일 실시형태에서, 삽입된 전이유전자는 예를 들어 마우스, 랫트, 토끼, 낙타, 라마 또는 유사로부터 비인간 단백질, 폴리펩타이드 또는 펩타이드(예컨대, 비인간 포유류 단백질, 폴리펩타이드 또는 펩타이드) 또는 RNA 분자를 암호화한다. 유리하게는, 본 개시내용의 바이러스는 전이유전자가 암성 세포 내부로 수송되게 한다. 따라서, 비인간 서열(예컨대, 단백질)에 대해 인간 환자에 의해 생성된 반응은 이 세포내 전달에 의해 최소화될 수 있다.
DNA 서열은 1개 초과의 전이유전자, 예를 들어 1개, 2개, 3개 또는 4개, 예컨대 1개 또는 2개의 전이유전자를 포함할 수 있다.
전이유전자 카세트는 1개 초과의 전이유전자, 예를 들어 1개, 2개, 3개 또는 4개, 예컨대 1개 또는 2개의 전이유전자를 포함할 수 있다.
본 명세서에 사용된 바와 같은, 전이유전자 카세트는 하나 이상의 암호화 서열의 형태의 하나 이상의 전이유전자 및 하나 이상의 조절 구성요소를 암호화하는 DNA 서열을 의미한다.
전이유전자 카세트는 하나 이상의 단일시스트론성 및/또는 폴리시스트론성 mRNA 서열을 암호화할 수 있다.
일 실시형태에서, 전이유전자 또는 전이유전자 카세트는 단일시스트론성 또는 폴리시스트론성 mRNA를 암호화하고, 예를 들어 카세트는 내인성 프로모터 또는 외인성 프로모터 또는 이들의 조합의 제어 하에 위치에서 아데노바이러스 게놈으로의 삽입에 적합하다.
본 명세서에 사용된 바와 같은, 단일시스트론성 mRNA는 단일 기능적 RNA, 펩타이드, 폴리펩타이드 또는 단백질을 암호화하는 mRNA 분자를 의미한다.
일 실시형태에서, 전이유전자 카세트는 단일시스트론성 mRNA를 암호화한다.
일 실시형태에서, 단일시스트론성 mRNA를 암호화하는 카세트의 맥락에서 전이유전자 카세트는 관심 있는 단백질에 대한 cDNA로부터 보통 유래한, 암호화 서열(즉, 전이유전자)인 (전이유전자가 어디서 및 언제 활성인지를 결정하는 조절 서열인) 외인성 프로모터 또는 (mRNA 분자가 언제 스플라이소솜에 의해 분해되는지를 결정하는 조절 서열인) 스플라이스 부위를 임의로 함유하고, 폴리A 신호 서열 및 종결자 서열을 임의로 함유하는, DNA의 분절을 의미한다.
일 실시형태에서, 전이유전자 카세트는 하나 이상의 폴리시스트론성 mRNA 서열을 암호화할 수 있다.
본 명세서에 사용된 바와 같은, 폴리시스트론성 mRNA는 2개 이상의 기능적 RNA, 펩타이드 또는 단백질 또는 이들의 조합을 암호화하는 mRNA 분자를 의미한다. 일 실시형태에서, 전이유전자 카세트는 폴리시스트론성 mRNA를 암호화한다.
일 실시형태에서, 폴리시스트론성 mRNA를 암호화하는 카세트의 맥락에서 전이유전자 카세트는 관심 있는 단백질 또는 펩타이드에 대한 cDNA로부터 보통 유래한, 2개 이상의 암호화 서열(즉, 전이유전자)(예를 들어 여기서 각각의 암호화 서열은 IRES 또는 2A 펩타이드에 의해 분리됨)인 (전이유전자가 어디서 및 언제 활성인지를 결정하는 조절 서열인) 외인성 프로모터 또는 (mRNA 분자가 언제 스플라이소솜에 의해 분해되는지를 결정하는 조절 서열인) 스플라이스 부위를 임의로 함유하는, DNA의 분절을 포함한다. 전사되는 마지막 암호화 서열 후, 카세트는 폴리A 서열 및 종결자 서열을 임의로 함유할 수 있다.
일 실시형태에서, 전이유전자 카세트는 단일시스트론성 mRNA, 이어서 폴리시스트론성 mRNA를 암호화한다. 또 다른 실시형태에서, 전이유전자 카세트는 폴리시스트론성 mRNA, 이어서 단일시스트론성 mRNA를 암호화한다.
일 실시형태에서, BX는 제한 부위, 예를 들어 1개, 2개, 3개 또는 4개, 예컨대 1개 또는 2개의 제한 부위를 포함한다. 일 실시형태에서, BX는 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함한다. 일 실시형태에서, BX는 특히 유전자 또는 유전자를 포함하는 DNA 서열을 게놈으로부터 구체적으로 절제하고/하거나 대체하도록 허용하도록 제한 부위가 이들을 샌드위칭하는 경우 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자 및 1개 이상의 제한 부위, 예를 들어 2개 또는 3개의 제한 부위를 포함한다. 대안적으로, 제한 부위는 각각의 유전자를 샌드위칭할 수 있고, 예를 들어 2개의 전이유전자가 존재할 때, 유전자가 선택적으로 절제되고/되거나 대체될 수 있도록 보장하도록 3개의 상이한 제한 부위가 필요하다. 일 실시형태에서, 1개 이상의, 예를 들어 모든 전이유전자는 전이유전자 카세트의 형태이다. 일 실시형태에서, BX는 서열 번호 10을 포함한다. 일 실시형태에서, 서열 번호 10은 예를 들어 전이유전자에 의해 중단된다. 실시형태에서, 서열 번호 10은 중단되지 않는다. 일 실시형태에서, BX는 제한 부위를 포함하지 않는다. 일 실시형태에서, BX는 결합이다. 일 실시형태에서, BX는 1개 이상의 전이유전자를 포함하거나 이들로 이루어진다.
일 실시형태에서, BY는 제한 부위, 예를 들어 1개, 2개, 3개 또는 4개, 예컨대 1개 또는 2개의 제한 부위를 포함한다. 일 실시형태에서, BY는 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함한다. 일 실시형태에서, BY는 특히 유전자 또는 유전자를 포함하는 DNA 서열을 게놈으로부터 구체적으로 절제하고/하거나 대체하도록 허용하도록 제한 부위가 이들을 샌드위칭하는 경우 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자 및 1개 이상의 제한 부위, 예를 들어 2개 또는 3개의 제한 부위를 포함한다. 대안적으로, 제한 부위는 각각의 유전자를 샌드위칭할 수 있고, 예를 들어 2개의 전이유전자가 존재할 때, 유전자가 선택적으로 절제되고/되거나 대체될 수 있도록 보장하도록 3개의 상이한 제한 부위가 필요하다. 일 실시형태에서, 1개 이상의, 예를 들어 모든 전이유전자는 전이유전자 카세트의 형태이다. 일 실시형태에서, BY는 서열 번호 11을 포함한다. 일 실시형태에서, 서열 번호 11은 예를 들어 전이유전자에 의해 중단된다. 실시형태에서, 서열 번호 11은 중단되지 않는다. 일 실시형태에서, BY는 제한 부위를 포함하지 않는다. 일 실시형태에서, BY는 결합이다. 일 실시형태에서, BY는 1개 이상의 전이유전자를 포함하거나 이들로 이루어진다.
일 실시형태에서, BX 및 BY는 각각 제한 부위, 예를 들어 1개, 2개, 3개 또는 4개, 예컨대 1개 또는 2개의 제한 부위를 포함한다. 일 실시형태에서, BX 및 BY는 각각 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함한다. 일 실시형태에서, BX 및 BY는 각각 특히 유전자 또는 유전자를 포함하는 DNA 서열을 게놈으로부터 구체적으로 절제하고/하거나 대체하도록 허용하도록 제한 부위가 이들을 샌드위칭하는 경우 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자 및 1개 이상의 제한 부위, 예를 들어 2개 또는 3개의 제한 부위를 포함한다. 대안적으로, 제한 부위는 각각의 유전자를 샌드위칭할 수 있고, 예를 들어 2개의 전이유전자가 존재할 때, 유전자가 선택적으로 절제되고/되거나 대체될 수 있도록 보장하도록 3개의 상이한 제한 부위가 필요하다. 일 실시형태에서, 1개 이상의, 예를 들어 모든 전이유전자는 전이유전자 카세트의 형태이다. 일 실시형태에서, BX 및 BY는 각각 서열 번호 10 및 서열 번호 11을 포함한다. 일 실시형태에서, BX 및 BY는 제한 부위를 포함하지 않는다. 일 실시형태에서, BX는 결합이고, BY는 결합이 아니다. 일 실시형태에서, BY는 결합이고, BX는 결합이 아니다.
일 실시형태에서, 전이유전자는 BX에 위치한다. 일 실시형태에서, 전이유전자 또는 전이유전자 카세트는 BY에 위치한다. 일 실시형태에서, 전이유전자 또는 전이유전자 카세트는 BX 및 BY에 위치하고, 예를 들어 전이유전자는 각각의 위치에서 동일하거나 상이할 수 있다.
유리하게는, 본 바이러스 작제물에서 전이유전자는 조기 유전자로부터 제거된 위치에서 삽입되는데, 왜냐하면 이것이 바이러스 유전자 발현 또는 복제의 속도에 영향을 미칠 가능성을 감소시키기 때문이다.
독립적인 일 양태에서, 혈청형 11의 복제 가능 종양 아데노바이러스 또는 섬유, 헥손 및 캡시드가 혈청형 11인 이의 바이러스 유도체가 제공되고, 여기서 바이러스 게놈은 치료학적 항체 또는 항체 결합 단편을 암호화하는 DNA 서열을 포함하고, 상기 DNA 서열은 E4 및 주요 후기 프로모터로 이루어진 것으로부터 선택된 아데노바이러스에 내인성인 프로모터의 제어 하에 있어서, 전이유전자는 바이러스 복제를 방해하지 않고, 예를 들어 치료학적 항체 또는 항체 결합 단편을 암호화하는 DNA 서열은 E4 프로모터의 제어 하에 또는 대안적으로 주요 후기 프로모터의 제어 하에 있고, 특히 항체 또는 항체 결합 단편을 암호화하는 DNA 서열은 바이러스 게놈 서열에서 L5 후에(즉, 바이러스 서열의 3' 말단을 향해) 위치한다. 유리하게는, 내인성 프로모터를 사용하는 것은 전이유전자를 삽입하기 위해 이용 가능한 공간의 양을 최대화한다.
유리하게는, 이 프로모터의 제어 하에 있을 때 바이러스는 복제 가능하게 있고, 전장 항체 또는 적합한 결합 단편 또는 다른 단백질로서 항체를 또한 발현할 수 있다. 따라서, 선택된 항체 또는 다른 단백질은 암 세포에 의해 발현될 것이다. 내인성 프로모터를 사용하는 것은 유리할 수 있는데, 왜냐하면 이것이 항체, 단편 또는 다른 단백질을 발현하도록 혼입될 필요가 있는 전이유전자 카세트의 크기를 감소시키기 때문이고, 즉 카세트는 더 작을 수 있는데, 왜냐하면 외인성 프로모터가 포함될 필요가 없기 때문이다.
바이러스에서 내인성 프로모터를 사용하는 것은 치료학적 맥락에서 또한 유리할 수 있는데, 왜냐하면 전이유전자를 연속하여 전사시키고 부적절한 농도의 항체 또는 단편을 생성시킬 수 있는 구성적 외인성 프로모터와 반대로 바이러스가 복제할 때 전이유전자가 오직 발현되기 때문이다.
일 실시형태에서, 항체 또는 단편의 발현은 주요 후기 프로모터의 제어 하에 있다.
일 실시형태에서, 항체 또는 단편의 발현은 E4 프로모터의 제어 하에 있다.
독립적인 일 양태에서, 혈청형 11의 복제 가능 종양 아데노바이러스 또는 섬유, 헥손 및 캡시드가 혈청형 11인 이의 바이러스 유도체가 제공되고, 여기서 바이러스 게놈은 바이러스 복제 주기에서 후기에 발현된 바이러스 게놈의 부분에 위치한 치료학적 항체 또는 항체 결합 단편을 암호화하는 DNA 서열을 포함하고, 그래서 전이유전자는 바이러스 복제를 방해하지 않고, 상기 DNA 서열은 아데노바이러스에 외인성인 프로모터의 제어 하에 있고, 예를 들어 치료학적 항체 또는 항체 결합 단편을 암호화하는 DNA 서열은 CMV 프로모터의 제어 하에 있고, 특히 항체 또는 항체 결합 단편을 암호화하는 DNA 서열은 바이러스 게놈 서열에서 L5 후에(즉, 바이러스 서열의 3' 말단을 향해) 위치한다.
외인성 프로모터를 사용하는 것은 유리할 수 있는데, 왜냐하면 이것은 몇몇 상황에서, 예를 들어 환자가 매우 만연한 암을 가지는 경우 특히 유용할 수 있는 항체 또는 단편을 강하게 구성적으로 발현할 수 있기 때문이다.
일 실시형태에서, 항체 또는 단편의 발현은 CMV 프로모터의 제어 하에 있다.
일 실시형태에서, 외인성 프로모터는 이 DNA 서열과 연관되고, 예를 들어 항체 또는 단편을 암호화하는 발현 카세트의 일부이다.
일 실시형태에서, 항체 또는 단편을 암호화하는 DNA 서열은 바이러스 서열에서 L5 유전자 후에 위치한다. 유리하게는, 본 발명자들은, 바이러스의 생활 주기 또는 벡터의 안정성에 부정적으로 영향을 미치지 않으면서, 외인성 또는 내인성 프로모터의 제어 하에 다양한 전이유전자가 BX 및/또는 BY로 삽입될 수 있다는 것을 확립하였다.
일 실시형태에서, 전이유전자는 적어도 하나의 암호화 서열(즉, 적어도 하나의 전이유전자) 및 임의로 하기로부터 독립적으로 선택된 하나 이상의 구성요소를 포함하는 전이유전자 카세트의 부분이다:
ⅰ. 유전자 발현의 조절자, 예컨대 외인성 프로모터 또는 스플라이스 억셉터;
ⅱ. 내부 리보솜 유입(IRES) DNA 서열;
ⅲ. 높은 자가 절단 효율 2A 펩타이드를 암호화하는 DNA 서열;
ⅳ. 폴리아데닐화 서열을 암호화하는 DNA 서열, 및
ⅴ. 이들의 조합.
따라서, 일 실시형태에서, 전이유전자 카세트는 ⅰ) 또는 ⅱ) 또는 ⅲ) 또는 iv)를 포함한다.
일 실시형태에서, 전이유전자 카세트는 ⅰ)과 ⅱ), 또는 ⅰ)과 ⅲ), 또는 ⅰ)과 ⅳ), 또는 ⅱ)와 ⅲ), 또는 ⅱ)와 ⅳ), 또는 ⅲ)과 ⅳ)를 포함한다.
일 실시형태에서, 전이유전자 카세트는 ⅰ)과 ⅱ)와 ⅲ), 또는 ⅰ)과 ⅱ)와 ⅳ), 또는 ⅰ)과 ⅲ)과 ⅳ), 또는 ⅱ)와 ⅲ)과 ⅳ)를 포함한다.
일 실시형태에서, 전이유전자 카세트는 ⅰ)과 ⅱ)와 ⅲ)과 ⅳ)를 포함한다.
일 실시형태에서, 전이유전자 또는 전이유전자 카세트는 예를 들어 단백질 암호화 서열의 시작 시 mRNA의 번역을 돕는 코작(Kozak) 서열을 포함한다.
적절한 위치에서 아데노바이러스로 혼입될 때 ITR의 기능을 보유한다. 일 실시형태에서, 5'ITR은 서열 번호 12의 약 1bp 내지 138bp의 서열 또는 전체 길이에 걸쳐 이에 90%, 95%, 96%, 97%, 98% 또는 99% 동일한 서열, 특히 서열 번호 12의 약 1bp 내지 138bp로 이루어진 서열을 포함하거나 이들로 이루어진다.
본 명세서에 사용된 바와 같은, 5'ITR은 적절한 위치에서 아데노바이러스로 혼입될 때 ITR의 기능을 보유하는 아데노바이러스의 3' 말단으로부터의 ITR의 일부 또는 전부를 의미한다. 일 실시형태에서, 3'ITR은 서열 번호 12의 약 32189bp 내지 32326bp의 서열 또는 전체 길이에 걸쳐 이에 90%, 95%, 96%, 97%, 98% 또는 99% 동일한 서열, 특히 서열 번호 12의 약 32189bp 내지 32326bp로 이루어진 서열을 포함하거나 이들로 이루어진다.
본 명세서에 사용된 바와 같은, B1은 아데노바이러스로부터의 E1A의 일부 또는 전부, 아데노바이러스의 E1B 영역의 일부 또는 전부, 및 독립적으로 아데노바이러스의 E1A 및 E1B 영역의 일부 또는 전부를 암호화하는 DNA 서열을 의미한다.
B1이 결합할 때, E1A 및 E1B 서열은 바이러스로부터 생략될 것이다. 일 실시형태에서, B1은 결합되고 따라서 바이러스는 벡터이다.
일 실시형태에서, B1은 전이유전자를 추가로 포함한다. E1 영역이 E1 영역으로 파괴 방식으로(즉, 서열의 "중간"에서) 삽입될 수 있는 전이유전자를 수용할 수 있거나, 유전자 재료를 수용할 더 많은 여지를 제공하도록 E1 영역의 일부 또는 전부가 결실될 수 있다는 것이 당해 분야에 공지되어 있다.
본 명세서에 사용된 바와 같은, E1A는 아데노바이러스 E1A 영역의 일부 또는 전부를 암호화하는 DNA 서열을 의미한다. 여기서 후자는 폴리펩타이드/단백질 E1A를 의미한다. 이것은 E1A 유전자에 의해 암호화된 단백질이 보존적 또는 비보존적 아미노산 변화를 가지도록 돌연변이될 수 있어서, 이것은 야생형과 동일한 기능(즉, 상응하는 비돌연변이된 단백질); 야생형 단백질과 비교하여 기능의 증가; 야생형 단백질과 비교하여 기능의 감소, 예컨대 기능 없음을 가지거나; 야생형 단백질과 비교하여 새로운 기능을 가지거나, 적절한 바대로 이들의 조합이다.
본 명세서에 사용된 바와 같은, E1B는 아데노바이러스 E1B 영역(즉, 폴리펩타이드 또는 단백질)의 일부 또는 전부를 암호화하는 DNA 서열을 의미하고, 이것은 E1B 유전자/영역에 의해 암호화된 단백질이 보존적 또는 비보존적 아미노산 변화를 가지도록 돌연변이될 수 있어서, 이것은 야생형과 동일한 기능(즉, 상응하는 비돌연변이된 단백질); 야생형 단백질과 비교하여 기능의 증가; 야생형 단백질과 비교하여 기능의 감소, 예컨대 기능 없음을 가지거나; 야생형 단백질과 비교하여 새로운 기능을 가지거나, 적절한 바대로 이들의 조합이다.
따라서, B1은 야생형 E1 영역, 예컨대 야생형 E1A 및/또는 E1B에 대해 변형되거나 비변형될 수 있다. 당업자는 E1A 및/또는 E1B가 존재하거나 (부분) 결실되거나 돌연변이되는지를 용이하게 확인할 수 있다.
본 명세서에 사용된 바와 같은, 야생형은 공지된 아데노바이러스를 의미한다. 공지된 아데노바이러스는 서열이 이용 가능한지와 무관하게 확인되거나 명명된 것이다.
일 실시형태에서, B1은 서열 번호 12의 139bp 내지 3932bp의 서열을 가진다.
본 명세서에 사용된 바와 같은, BA는 적절한 바대로 임의의 비암호화 서열을 포함하는 E2B-L1-L2-L3-E2A-L4 영역을 암호화하는 DNA 서열을 의미한다. 일반적으로, 이 서열은 전이유전자를 포함하지 않을 것이다. 일 실시형태에서, 서열은 공지된 아데노바이러스로부터의 인접 서열, 예를 들어 표 1에 기재된 혈청형, 특히 B 그룹 바이러스, 예를 들어 Ad3, Ad7, Ad11, Ad14, Ad16, Ad21, Ad34, Ad35, Ad51 또는 이들의 조합, 예컨대 Ad3, Ad11 또는 이들의 조합과 실질적으로 유사하거나 동일하다. 일 실시형태에서, E2B-L1-L2-L3-E2A-L4는 이 구성요소 및 영역과 연관된 다른 구조 구성요소를 포함한다는 것을 의미하고, 예를 들어 BA는 일반적으로 하기한 것: IV2A IV2a-E2B-L1-L2-L3-E2A-L4와 같은 단백질 IV2a를 암호화하는 서열을 포함할 것이다.
일 실시형태에서, E2B 영역은 키메라이다. 즉, 2개 이상의 상이한 아데노바이러스 혈청형, 예를 들어 Ad3 및 Ad11, 예컨대 Ad11p로부터의 DNA 서열을 포함한다. 일 실시형태에서, E2B 영역은 서열 번호 12의 5068bp 내지 10355bp의 서열 또는 전체 길이에 걸쳐 이에 95%, 96%, 97%, 98% 또는 99% 동일한 서열을 가진다.
일 실시형태에서, 성분 BA에서의 E2B는 (WO2005/118825에 개시된 서열 번호 3에 상응하는) 서열 번호 47에 도시된 서열을 포함한다.
일 실시형태에서, BA는 서열 번호 12의 3933bp 내지 27184bp의 서열을 가진다.
본 명세서에 사용된 바와 같은, E3은 아데노바이러스 E3 영역(즉, 단백질/폴리펩타이드)의 일부 또는 전부를 암호화하는 DNA 서열을 의미하고, 이것은 E3 유전자에 의해 암호화된 단백질이 보존적 또는 비보존적 아미노산 변화를 가지도록 돌연변이될 수 있어서, 이것은 야생형과 동일한 기능(즉, 상응하는 비돌연변이된 단백질); 야생형 단백질과 비교하여 기능의 증가; 야생형 단백질과 비교하여 기능의 감소, 예컨대 기능 없음을 가지거나, 야생형 단백질과 비교하여 새로운 기능을 가지거나, 적절한 바대로 이들의 조합이다.
일 실시형태에서, E3 영역은 표 1에 제공된 아데노바이러스 혈청형 또는 이들의 조합, 특히 B 그룹 혈청형, 예를 들어 Ad3, Ad7, Ad11(특히 Ad11p), Ad14, Ad16, Ad21, Ad34, Ad35, Ad51 또는 이들의 조합, 예컨대 Ad3, Ad11(특히 Ad11p) 또는 이들의 조합의 형태이다.
일 실시형태에서, E3 영역이 부분적으로 결실되고, 예를 들어 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10%, 5% 결실된다.
일 실시형태에서, B2가 결합이고, E3 영역을 암호화하는 DNA가 부재한다.
일 실시형태에서, E3 영역을 암호화하는 DNA는 전이유전자에 의해 대체되거나 차단될 수 있다. 본 명세서에 사용된 바와 같은, 전이유전자에 의해 대체된, 본 명세서에 사용된 바와 같은, E3 영역은 전이유전자에 의해 대체된 E3 영역의 일부 또는 전부를 포함한다.
일 실시형태에서, B2 영역은 서열 번호 12의 27185bp 내지 28165bp의 서열을 포함한다.
일 실시형태에서, B2는 서열 번호 12의 27185bp 내지 28165bp의 서열로 이루어진다.
본 명세서에 사용된 바와 같은, BX는 BB의 L5 유전자의 5' 말단의 근처에서의 DNA 서열을 의미한다. 본 명세서에 사용된 바와 같은, L5 유전자의 5' 말단에 근처에서 또는 근위에서는 L5 유전자의 5' 말단에 인접한(근접한) 또는 이것과 고유하게 연관된, 즉 L5 유전자의 5' 프라임 말단에 접하거나 근접한 비암호화 영역 또는 이것과 고유하게 연관된 비암호화 영역을 의미한다. 대안적으로, 근처에서 또는 근위에서는 BX 영역과 L5 유전자의 5' 말단 사이에 암호화 서열이 없도록 L5 유전자에 가깝다는 것을 의미할 수 있다.
따라서, 일 실시형태에서, BX는 예를 들어 L5 유전자의 암호화 서열의 시작을 나타내는 L5의 염기에 직접적으로 연결된다.
따라서, 일 실시형태에서, BX는 예를 들어 비암호화 서열의 시작을 나타내는 L5의 염기에 직접적으로 연결되거나, L5와 자연에서 연관된 비암호화 영역에 직접적으로 연결된다. 본 명세서에 사용된 바와 같은, L5와 자연에서 연관된 비암호화 영역은 L5 유전자의 일부이거나 이것과 인접하지만 또 다른 유전자의 일부가 아닌 비암호화 영역의 전부 중 일부를 의미한다.
일 실시형태에서, BX는 서열 번호 10의 서열을 포함한다. 이 서열은 인공 비암호화 서열이고, 여기서 예를 들어 전이유전자(또는 전이유전자 카세트), 제한 부위 또는 이들의 조합을 포함하는 DNA 서열이 내부에 삽입될 수 있다. 이 서열은 유리한데, 왜냐하면 이것이 바이러스 안정성 및 생존능력에 대한 파괴적인 영향을 최소화하면서 전이유전자의 정확한 위치에 대한 약간의 가요성을 허용한다는 점에서 완충제로서 작용하기 때문이다.
삽입체(들)는 5' 말단으로부터, 3' 말단으로부터 서열 번호 10 내에 어디든 또는 bp 1 내지 201 사이, 예를 들어 염기 쌍 1/2, 2/3, 3/4, 4/5, 5/6, 6/7, 7/8, 8/9, 9/10, 10/11, 11/12, 12/13, 13/14, 14/15, 15/16, 16/17, 17/18, 18/19, 19/20, 20/21, 21/22, 22/23, 23/24, 24/25, 25/26, 26/27, 27/28, 28/29, 29/30, 30/31, 31/32, 32/33, 33/34, 34/35, 35/36, 36/37, 37/38, 38/39, 39/40, 40/41, 41/42, 42/43, 43/44, 44/45, 45/46, 46/47, 47/48, 48/49, 49/50, 50/51, 51/52, 52/53, 53/54, 54/55, 55/56, 56/57, 57/58, 58/59, 59/60, 60/61, 61/62, 62/63, 63/64, 64/65, 65/66, 66/67, 67/68, 68/69, 69/70, 70/71, 71/72, 72/73, 73/74, 74/75, 75/76, 76/77, 77/78, 78/79, 79/80, 80/81, 81/82, 82/83, 83/84, 84/85, 85/86, 86/87, 87/88, 88/89, 89/90, 90/91, 91/92, 92/93, 93/94, 94/95, 95/96, 96/97, 97/98, 98/99, 99/100, 100/101, 101/102, 102/103, 103/104, 104/105, 105/106, 106/107, 107/108, 108/109, 109/110, 110/111, 111/112, 112/113, 113/114, 114/115, 115/116, 116/117, 117/118, 118/119, 119/120, 120/121, 121/122, 122/123, 123/124, 124/125, 125/126, 126/127, 127/128, 128/129, 129/130, 130/131, 131/132, 132/133, 133/134, 134/135, 135/136, 136/137, 137/138, 138/139, 139/140, 140/141, 141/142, 142/143, 143/144, 144/145, 145/146, 146/147, 147/148, 148/149, 150/151, 151/152, 152/153, 153/154, 154/155, 155/156, 156/157, 157/158, 158/159, 159/160, 160/161, 161/162, 162/163, 163/164, 164/165, 165/166, 166/167, 167/168, 168/169, 169/170, 170/171, 171/172, 172/173, 173/174, 174/175, 175/176, 176/177, 177/178, 178/179, 179/180, 180/181, 181/182, 182/183, 183/184, 184/185, 185/186, 186/187, 187/188, 189/190, 190/191, 191/192, 192/193, 193/194, 194/195, 195/196, 196/197, 197/198, 198/199, 199/200 또는 200/201 사이의 임의의 지점에 발생할 수 있다.
일 실시형태에서, BX는 bp 27 내지 bp 28에 삽입된 DNA 서열을 가지는 서열 번호 10 또는 서열 번호 12의 위치 28192bp 내지 28193bp에 상응하는 장소를 포함한다.
일 실시형태에서, 삽입체는 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개 또는 2개의 제한 부위를 포함한다. 일 실시형태에서, 제한 부위는 2개의 제한 부위를 포함하는 19bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개의 제한 부위를 포함하는 9bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개 또는 2개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함한다. 일 실시형태에서, 제한 부위는 2개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함하는 19bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개, 2개 또는 3개, 예컨대 1개 또는 2개의 전이유전자를 포함하는 9bp 제한 부위 삽입체이다. 일 실시형태에서, 2개의 제한 부위는 (예를 들어, 전이유전자 카세트에서) 1개 이상, 예컨대 2개의 전이유전자를 샌드위칭한다. 일 실시형태에서, BX가 2개의 제한 부위를 포함할 때, 상기 제한 부위는 서로 상이하다. 일 실시형태에서, BX에서 상기 1개 이상의 제한 부위는 이것이 삽입되는 특정한 아데노바이러스 게놈에서 천연 비발생이다. 일 실시형태에서, BX에서 상기 1개 이상의 제한 부위는, 예를 들어 천연 발생 제한 부위 및/또는 게놈의 다른 부분으로 도입된 제한 부위, 예컨대 BY로 도입된 제한 부위와 다른, 아데노바이러스 게놈에서 그 외 위치한 다른 제한 부위와 다르다. 따라서, 일 실시형태에서, 제한 부위 또는 부위들은 섹션에서의 DNA가 특이적으로 절단되게 한다.
유리하게는, "독특한" 제한 부위의 사용은 단순히 적절한 제한 효소를 사용함으로써 바이러스 게놈이 절단되는 제어 및 선택도를 제공한다.
본 명세서에 사용된 바와 같은, 특이적으로 절단된은 제한 부위에 특이적인 효소의 사용이 원하는 위치, 때때로 이것이 위치의 쌍일 수 있지만, 보통 일 위치에서만 바이러스를 절단하는 경우를 의미한다. 본 명세서에 사용된 바와 같은, 한 쌍의 위치는 동일한 효소(즉, 서로 구별될 수 없음)에 의해 절단되도록 설계된 서로에 근접한 2개의 제한 부위를 의미한다.
일 실시형태에서, 제한 부위 삽입체는 서열 번호 55이다.
일 실시형태에서, BX는 서열 번호 12의 28166bp 내지 28366bp의 서열을 가진다.
일 실시형태에서, BX는 결합이다.
본 명세서에 사용된 바와 같은, BB는 L5 영역을 암호화하는 DNA 서열을 의미한다. 본 명세서에 사용된 바와 같이, L5 영역은 문맥에서 적절한 바대로 섬유 폴리펩타이드/단백질을 암호화하는 유전자를 함유하는 DNA 서열을 의미한다. 섬유 유전자/영역은 아데노바이러스의 주요 캡시드 성분인 섬유 단백질을 암호화한다. 섬유는 수용체 인식에서 작용하고, 세포에 선택적으로 결합하고 이를 감염시키는 아데노바이러스의 능력에 기여한다.
본 개시내용의 바이러스에서, 섬유는 임의의 아데노바이러스 혈청형 유래일 수 있고, 상이한 혈청형 중 하나에 대한 섬유를 변경하는 것의 결과로서 키메라인 아데노바이러스는 공지되어 있다. 일 실시형태에서, 섬유는 B 그룹 바이러스, 특히 Ad11, 예컨대 Ad11p 유래이다.
일 실시형태에서, BB는 서열 번호 12의 28367bp 내지 29344bp의 서열을 가진다.
본 명세서에 사용된 바와 같은, BY와 관련하여 DNA 서열은 BB의 L5 유전자의 3' 말단의 근처에서의 DNA 서열을 의미한다. 본 명세서에 사용된 바와 같은, L5 유전자의 3' 말단에 근처에서 또는 근위에서는 L5 유전자의 3' 말단에 인접한(근접한) 또는 이것과 고유하게 연관된, 즉 L5 유전자의 3' 프라임 말단에 접하거나 근접한 비암호화 영역 또는 이것과 고유하게 연관된 비암호화 영역(즉, L5에 내인성인 비암호화 서열의 전부 또는 일부)을 의미한다. 대안적으로, 근처에서 또는 근위에서는 BY 영역과 L5 유전자의 3' 말단 사이에 암호화 서열이 없도록 L5 유전자에 가깝다는 것을 의미할 수 있다.
따라서, 일 실시형태에서, BY는 암호화 서열의 "말단"을 나타내는 L5의 염기에 직접적으로 연결된다.
따라서, 일 실시형태에서, BY는 비암호화 서열의 "말단"을 나타내는 L5의 염기에 직접적으로 연결되거나, L5와 천연으로 연관된 비암호화 영역에 직접적으로 연결된다.
고유하게 및 천연으로는 본 명세서에서 상호 교환되어 사용된다. 일 실시형태에서, BY는 서열 번호 11의 서열을 포함한다. 이 서열은 예를 들어 전이유전자(또는 전이유전자 카세트), 제한 부위 또는 이들의 조합을 포함하는 DNA 서열이 삽입될 수 있는 비암호화 서열이다. 이 서열은 유리한데, 왜냐하면 이것이 바이러스 안정성 및 생존능력에 파괴 효과를 최소화하면서 전이유전자의 정확한 위치에서 약간의 가요성을 허용하는 데 있어서 완충제로서 작용하기 때문이다.
삽입체(들)는 5' 말단으로부터, 3' 말단으로부터 서열 번호 11 내에 어디든 또는 bp 1 내지 35 사이, 예를 들어 염기 쌍 1/2, 2/3, 3/4, 4/5, 5/6, 6/7, 7/8, 8/9, 9/10, 10/11, 11/12, 12/13, 13/14, 14/15, 15/16, 16/17, 17/18, 18/19, 19/20, 20/21, 21/22, 22/23, 23/24, 24/25, 25/26, 26/27, 27/28, 28/29, 29/30, 30/31, 31/32, 32/33, 33/34, 또는 34/35 사이의 임의의 지점에 발생할 수 있다.
일 실시형태에서, BY는 서열 번호 12에서 bp 12와 13 사이의 위치 또는 29356bp 및 29357bp에 상응하는 위치 사이에 삽입된 DNA 서열을 가지는 서열 번호 11을 포함한다. 일 실시형태에서, 삽입체는 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개 또는 2개의 제한 부위를 포함한다. 일 실시형태에서, 제한 부위는 2개의 제한 부위를 포함하는 19bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개의 제한 부위를 포함하는 9bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개 또는 2개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 또는 3개의 전이유전자, 예컨대 1개 또는 2개의 전이유전자를 포함한다. 일 실시형태에서, 제한 부위는 2개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함하는 19bp 제한 부위 삽입체이다. 일 실시형태에서, 제한 부위 삽입체는 1개의 제한 부위 및 적어도 1개의 전이유전자, 예를 들어 1개 또는 2개의 전이유전자를 포함하는 9bp 제한 부위 삽입체이다. 일 실시형태에서, 2개의 제한 부위는 (예를 들어, 전이유전자 카세트에서) 1개 이상, 예컨대 2개의 전이유전자를 샌드위칭한다. 일 실시형태에서, BY가 2개의 제한 부위를 포함할 때, 상기 제한 부위는 서로 다르다. 일 실시형태에서, BY 내의 상기 1개 이상의 제한 부위는 이들이 삽입된 특정한 아데노바이러스 게놈에서 천연 비발생이다(예컨대, 독특하다). 일 실시형태에서, BY 내의 상기 1개 이상의 제한 부위는 아데노바이러스 게놈에서 그 외 위치한 다른 제한 부위와 다르고, 예를 들어 천연 발생 제한 부위 또는 BX와 같은 게놈의 다른 부분으로 도입된 제한 부위와 다르다. 따라서, 일 실시형태에서, 제한 부위 또는 부위들은 섹션에서의 DNA가 특이적으로 절단되게 허용한다.
일 실시형태에서, 제한 부위 삽입체는 서열 번호 54이다.
일 실시형태에서, BY는 서열 번호 12의 29345bp 내지 29379bp의 서열을 가진다.
일 실시형태에서, BY는 결합이다.
일 실시형태에서, 삽입체는 서열 번호 11에서 bp 12 후이다.
일 실시형태에서, 삽입체는 서열 번호 12의 약 29356bp 위치이다.
일 실시형태에서, 삽입체는 1개 이상, 예를 들어 1개, 2개 또는 3개, 예컨대 1 또는 2개의 전이유전자를 포함하는 전이유전자 카세트이다.
본 명세서에 사용된 바와 같은, E4는, E4 유전자에 의해 암호화된 단백질이 보존적 또는 비보존적 아미노산 변경을 가지고, 야생형(상응하는 비돌연변이된 단백질)과 동일한 기능; 야생형 단백질과 비교하여 기능의 증가; 야생형 단백질과 비교하여 기능의 감소, 예컨대 기능 없음을 가지거나, 야생형 단백질과 비교하여 새로운 기능을 가지거나, 적절한 바대로 이들의 조합이도록, 돌연변이될 수 있는, 아데노바이러스 E4 영역(즉, 폴리펩타이드/단백질 영역)의 일부 또는 전부를 암호화하는 DNA 서열을 의미한다.
일 실시형태에서, E4 영역은 부분적으로 결실되고, 예를 들어 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10% 또는 5% 결실된다. 일 실시형태에서, E4 영역은 서열 번호 12의 32188bp 내지 29380bp의 서열을 가진다.
일 실시형태에서, B3는 결합이고, 즉 E4는 부재한다.
일 실시형태에서, B3은 서열 번호 12의 32188bp 내지 29380bp로 이루어진 서열을 가진다.
본 명세서에 사용된 바와 같은, 숫자 범위는 종점을 포함한다.
당업자는 본 명세서에서의 화학식, 예컨대 화학식 (I)에서의 구성요소가 인접하고 비암호화 DNA 서열, 및 본 명세서에 언급된 유전자 및 암호화 DNA 서열(구조 특징부)을 구현할 수 있다는 것을 이해할 것이다. 하나 이상의 실시형태에서, 본 개시내용의 화학식은 아데노바이러스 게놈에서 천연 발생 서열을 기술하도록 시도한다. 이 맥락에서, 화학식이 게놈의 관련 섹션을 규명하는 주요 구성요소를 언급하고, DNA의 게놈 스트레치의 철저한 설명인 것으로 의도되지 않는다는 것이 당업자에게 명확할 것이다.
본 명세서에 사용된 바와 같은, E1A, E1B, E3 및 E4는 각각 독립적으로 본 명세서에 기재된 바와 같은 각각의 영역의 야생형 및 이의 균등물, 돌연변이된 또는 부분적으로 결실된 형태, 특히 공지된 아데노바이러스로부터의 야생형 서열을 의미한다.
본 명세서에 사용된 바와 같은, "삽입체"는 이것이 기준 서열을 중단시키도록 5' 말단에, 3' 말단에 또는 소정의 DNA 서열 기준 분절 내에 혼입된 DNA 서열을 의미한다. 후자는 삽입체가 위치한 기준점으로서 사용되는 기준 서열이다. 본 개시내용의 맥락에서, 삽입체는 일반적으로 서열 번호 10 또는 서열 번호 11 내에 발생한다. 삽입체는 제한 부위 삽입체, 전이유전자 카세트 또는 둘 다일 수 있다. 서열이 중단될 때, 바이러스는 여전히 원래 서열을 포함할 것이지만, 일반적으로 이것은 삽입체를 샌드위칭하는 2개의 단편으로 있을 것이다.
일 실시형태에서, 전이유전자 또는 전이유전자 카세트는 비편향 삽입 전이인자, 예컨대 Tn7 전이인자 또는 이의 부분을 포함하지 않는다. 본 명세서에 사용된 바와 같은, Tn7 전이인자는 WO2008/080003에 기재된 바와 같은 비편향 삽입 전이인자를 의미한다.
본 명세서에 사용된 바와 같은, 제조의 연속 공정은, 예를 들어 공정에서 적어도 1개의 시점에서 또는 적어도 2개의 시점에서 생성된 바이러스 입자가 세포마다 50,000개 이상일 때, 특히 각각의 세포에 의해 생성된 바이러스, 예를 들어 본 명세서에 기재된 바이러스, 예컨대 복제 가능 키메라 종양 아데노바이러스(여기서, 바이러스는 2회 이상의 복제 주기를 가짐)가 비연속 공정과 비교하여 증가하도록, 본 개시내용에 따라 정의된 바와 같은 바이러스의 제조 공정이다.
연속 공정은 별개의 배양의 반대이고, 여기서 감염 후 세포는 추가의 세포에 보충되지 않고, 세포는 복제된 바이러스를 수확하도록 예를 들어 용해되거나, 세포는 단일 바이러스 복제 주기 및 이로부터 바이러스의 회수 후 버려진다. 본 개시내용의 공정의 일부로서, 바이러스 함유 세포 비함유 상청액은 바이러스의 다운스트림 정제를 위해 다회 또는 연속하여 수확될 수 있다. 일 실시형태에서, 수확은 세포 배양의 기간의 종료 시가 아니다.
일 실시형태에서, 일 이상의 시점에서 세포마다 제조된 바이러스 입자는 적어도 75,000개, 예를 들어 80,000개; 90,000개; 100,000개; 150,000개; 175,000개; 180,000개 또는 195,000개이다.
이 범위에서 세포마다 바이러스 수율은 소정의 바이러스에 대해 관련 매개변수를 선택함으로써 달성된다. 이 수율을 달성하는 데 중요한 매개변수는 시작 시드 밀도, 감염 다중도(MOI), 배지의 변경 및 공정의 기간이다. 이론에 구속되고자 바라지 않으면서, 이 매개변수의 제어가 수율과 관련하여 공정에 대해 제어를 허용하고, 바이러스 생성물이 즉 상청액, 세포 또는 둘 다에서 위치하는 곳에 대해 제어를 또한 제공할 수 있는 것으로 생각된다.
본 명세서에 사용된 바와 같은, 낮은 감염 다중도는 20 미만, 예컨대 19, 18, 17, 16, 15, 14, 13, 12.5, 12, 11, 10, 9, 8, 7, 6 또는 5의 감염 다중도를 의미한다.
본 명세서에 사용된 바와 같은, 높은 감염 다중도는 45 이상, 예컨대 46, 47, 48, 49, 50, 51, 52, 53, 54, 55 이상, 예컨대 100의 감염 다중도를 의미한다.
본 명세서에 사용된 바와 같은, 낮은 시드 밀도는 2×106 이하, 예컨대 1.9, 1.8, 1.7, 1.6, 1.5, 1.4, 1.3, 1.2, 1.1, 1.0, 0.9, 0.8, 0.7, 0.6 또는 0.5×106 이하의 시드 밀도를 의미한다.
본 명세서에 사용된 바와 같은, 높은 시드 밀도는 3.5×106 이상, 예를 들어 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5×106 이상이다.
상기 공정의 일 실시형태에서, 포유류 세포는 1 내지 9×104vp/㎖ 이상, 예컨대 1 내지 9×105, 1 내지 9×106, 1 내지 9×107, 1 내지 9×108, 1 내지 9×109, 특히 1-5×106vp/㎖ 또는 2.5-5×108vp/㎖의 바이러스의 시작 농도에 의해 감염된다.
일 실시형태에서, 포유류 세포는 1 내지 4×106vp/㎖, 예컨대 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.5, 3.0 또는 4×106vp/㎖의 시작 시드 밀도에 의해 감염된다.
상기 공정의 일 실시형태에서, 포유류 세포는 약 1 내지 200ppc, 예를 들어 40 내지 120ppc, 예컨대 50ppc에서 1×106vp/㎖의 시작 농도에 의해 감염된다.
본 명세서에 사용된 바와 같은, ppc는 세포마다 바이러스 입자의 수를 의미한다. ppc 및 감염 다중도는 본 명세서에서 상호교환되어 사용된다.
일 실시형태에서, 배양 동안 바이러스, 예컨대 키메라 종양 아데노바이러스는 세포마다 20 내지 150개의 입자(ppc), 예컨대 40 내지 100ppc, 특히 50ppc의 범위의 농도이다. 이 농도는 시작 시드 밀도와 반대인 배양 공정 동안의 농도이다. 바이러스 농도의 더 낮은 값, 예를 들어 100ppc 미만, 예컨대 50ppc, 특히 20 이하, 예컨대 15, 14, 13, 12.5, 12, 11 또는 10이 유리할 수 있다. 이점은 더 높은 바이러스 농도를 가지는 배양과 비교하여 증가한 세포 생존능력(특히 세포 생존능력이 수확 전에 측정될 때), 및 세포마다 증가한 수준의 바이러스 입자 생성의 하기 특성 중 하나 이상을 포함할 수 있다.
일 실시형태에서, 배양물 내의 세포의 수는 배양물 중의 바이러스의 수준에 상응하도록 조정되고, 예를 들어 세포는 배양물(예컨대, 정지 상의 세포)에 첨가되거나, 배양물의 세포 성장은 감염 다중도(MOI)를 거의 일정하게 유지시키는 세포 수를 제공하도록 조정된다.
바이러스 제조는 또한 세포 밀도에 따라 달라진다. 일 실시형태에서, 세포 밀도는 100만 내지 1000만 개의 세포/㎖, 예컨대 200만 내지 1000만 개의 세포/㎖의 범위이다.
본 명세서에 사용된 바와 같은, "공정의 종료를 향해"는 상기 공정이 감염 후 예를 들어 약 60시간, 예컨대 65시간, 70시간, 71시간, 72시간, 73시간, 74시간, 75시간, 76시간, 77시간, 78시간, 79시간, 80시간, 81시간, 82시간, 83시간, 84시간, 85시간, 86시간, 87시간, 88시간, 89시간 이상으로부터 실행되는 시간의 마지막 40%를 의미한다.
본 명세서에 사용된 바와 같은, "바이러스 감염 및 제조를 위한 생존가능 세포는 바이러스를 복제하기에 적합한 수준으로 용기에 유지된다"는 바이러스의 원해지고 유용하고 일반적인 수율을 생성하는 수준에서 생존가능 세포를 유지시키는 것을 의미한다.
생존가능 세포는 바이러스에 의해 감염될 수 있고/있거나, 복제 바이러스를 지지할 수 있는 세포, 특히 바이러스에 의해 감염될 수 있고/있거나, 복제 바이러스를 지지할 수 있는 건강한 세포이다.
낮은 세포 생존능력은 시간에 따라 바이러스를 공격할 수 있는 효소 또는 단백질에 세포를 노출시킬 있는 세포 용해를 발생시킬 수 있다. 그러나, 세포 배양과 같은 동적 공정에서 세포의 일정 백분율, 예컨대 적은 백분율이 용해될 수 있다. 이것은 실제 조건에서 상당한 문제점을 일반적으로 발생시키지 않는다.
비생존가능 세포는 바이러스 복제와 관련하여 죽거나, 용해되거나, 불활성일 것이다.
누출된 세포는 증가한 침투성을 가지는 막을 가지는 것이다.
흥미롭게도, 제조 공정 동안 배양물이 첨가된 세포는 예를 들어 약 24시간 내에, 즉 생존능력 염료에 의해 염색될 때 비교적 신속히 증가한 침투성을 발생시키는 것으로 보인다. 감염성 바이러스 입자를 포함하는 높은 수준의 바이러스 입자가 여전히 생성될 수 있으므로 이것은 불리한 것으로 보이지 않는다고 말해진다.
세포 생존능력을 시험하는 방법은 당해 분야의 당업자에게 공지되어 있고, 세포의 샘플을 위하는 것 및 비생존능력이고 또한 누출된 세포를 침투하는 세포를 침투하고 염색시키는 생존능력 염색과 시험하는 것을 포함한다.
세포 생존능력 검정은 하기 기법 중 하나 이상에 기초할 수 있다:
1. 세포용해 또는 막 누출 검정: 이 카테고리는 세포막이 더 이상 온전하지 않을 때 용이하게 검출될 수 있는 모든 세포에서의 안정한 효소인 락테이트 탈수소효소 검정을 포함한다. 예는 프로피듐 요오다이드, 트립판 블루 및 7-아미노악티노마이신 D를 포함한다.
2. 미토콘드리아 활성 또는 카스파제 검정: 레사주린 및 포르마잔(MTT/XTT)은 세포 사멸의 전조가 되는 아폽토시스 과정에서 다양한 단계에 대해 평가될 수 있다.
3. 기능적 검정: 세포 기능의 검정은 평가되는 세포의 유형에 매우 특이적일 것이다. 예를 들어, 이동성은 정자 세포 기능의 광범위하게 이용되는 검정이다. 생식력은 일반적으로 배우자 생존을 평가하기 위해 이용될 수 있다. 적혈구는 변형성, 삼투압 취약성, 용혈, ATP 수준 및 헤모글로빈 함량의 면에서 평가된다.
4. 게놈 및 단백질체 검정: 세포는 마이크로어레이 및 단백질 칩을 이용하여 스트레스 경로의 활성화에 대해 평가될 수 있다.
세포 생존능력을 평가하기 위해 이용된 시험의 일반 목록은 ATP 시험, 칼세인(Calcein) AM, 클로노게닉(Clonogenic) 검정, 에티듐 동종이합체 검정, 에반스(Evans) 블루, 플루오레세인 다이아세테이트 가수분해/프로피듐 요오다이드 염색(FDA/PI 염색), 유세포분석법, 포르마잔 기반 검정(MTT/XTT), 녹색 형광 단백질, 락테이트 탈수소효소(LDH), 메틸 바이올렛, 프로피듐 요오다이드, 괴사, 아폽토시스 및 정상 세포를 구별할 수 있는 DNA 염색, 레사주린, 트립판 블루, 생 세포 배제 염료(염료는 오직 죽은 세포의 세포막을 횡단함) 및 TUNEL 검정을 포함한다.
따라서, 세포의 생존능력은, 당해 분야의 당업자에게 공지된 기법을 이용하여 예를 들어 트립판 블루(및 광 현미경검사법) 또는 670㎚에서 방출하는 생체 염료인 7-아미노-악티노마이신 D(또는 ViaProbe, 7AAD의 상업용 사용 준비 용액)에 의한 세포 염색 및 유세포분석법에 의해 시험될 수 있다. 염색이 세포로 침투하는 경우, 세포는 생존가능하지 않다고 생각된다. 염료가 채워지지 않은 세포는 생존가능하다고 생각된다. 예시적인 방법은 대략 100㎕의 세포 현탁액마다 약 5㎕의 7AAD 및 약 5㎕의 of 아넥신(Annexin)-V(아폽토시스 동안 노출된 외부 인지질 포스파티딜세린에 결합한 인지질 결합 단백질)을 사용할 수 있다. 이 혼합물은 광의 부재 하에 약 15분 동안 주변 온도에서 항온처리될 수 있다. 분석은 이후 유세포분석법을 이용하여 수행될 수 있다. 예를 들어, 문헌[MG Wing, AMP Montgomery, S. Songsivilai and JV Watson. An Improved Method for the Detection of Cell Surface Angigens in Samples of Low Viability using Flow Cytometry. J Immunol Methods 126: 21-27 1990]을 참조한다.
일 실시형태에서, 산소 흡수 또는 산소 전달은 세포의 생존능력을 평가하기 위해 사용된다. 여전히 바이러스를 복제할 수 있는 누출된 세포가 시약, 예컨대 트립판 블루 등에 의해 염색될 수 있으므로, 이것은 세포의 생존능력을 평가하는 더 적절한 방법일 수 있다. 따라서, 이 방법은 누출된 세포에 대해 죽은/용해된 세포를 구별하도록 이용될 수 있다.
놀랍게도, 본 발명자들은, 공정의 몇몇 실시형태를 이용할 때, 특히 새로운 세포의 첨가가 없을 때 세포가 연속 제조에 대해 본 명세서에 기재된 기간 동안 감염 후 높은 생존능력(예컨대, 80 내지 90%의 생존능력은 이 맥락에서 생존능력 염료에 의해 염색되지 않음)을 유지한다는 것을 발견하였다. 따라서, 일 실시형태에서, 수확 및 공정은 충분한 세포가 계속 생존가능한 한 계속될 수 있다.
일 실시형태에서, 배지 교환 없이 그리고 바이러스 복제를 지지하는 세포의 첨가 또는 대체 없이, 세포 생존능력은 EnAd에 의해 감염될 때 96시간 시점(즉, 감염 후 96시간)에서 상기 공정 동안 대략 50% 내지 100%, 예를 들어 60% 내지 95%, 예컨대 90%의 생존능력(즉, 세포의 90%는 생존능력 염료에 의한 염색을 가지지 않음)이다.
일 실시형태에서, 세포 생존능력은 NG76에 의해 감염될 때 96시간 시점(즉, 감염 후 96시간)에서 상기 공정 동안 대략 50% 내지 100%, 예를 들어 60% 내지 90%, 특히 새로운 세포가 첨가되지 않을 때, 예컨대 83%의 생존능력(즉, 세포의 83%는 생존능력 염료에 의한 염색을 가지지 않음)이다.
일 실시형태에서, 세포 생존능력은 NG135에 의해 감염될 때 96시간 시점(즉, 감염 후 96시간)에서 상기 공정 동안 대략 50% 내지 100%, 예를 들어 60% 내지 90%, 특히 새로운 세포가 첨가되지 않을 때, 예컨대 85%의 생존능력(세포의 85%는 생존능력 염료에 의한 염색을 가지지 않음)이다.
일 실시형태에서, 세포 생존능력은 Ad11에 의해 감염될 때 96시간 시점(즉, 감염 후 96시간)에서 상기 공정 동안 대략 50% 내지 100%, 예를 들어 80% 내지 90%, 특히 새로운 세포가 첨가되지 않을 때, 예를 들어 85%의 생존능력(즉, 세포의 85%는 생존능력 염료에 의한 염색을 가지지 않음)이다.
일 실시형태에서, 배양 공정은 하나 이상의 세포 첨가 또는 변경을 포함한다. 본 명세서에 이용된 세포 첨가는 세포의 일부 또는 전부의 대체 및 임의로 죽은 세포의 제거를 의미한다. 본 명세서에 사용된 바와 같은, 세포 변경은 적어도 몇몇 세포의 제거 및 새로운 세포의 첨가를 의미한다.
일 실시형태에서, 연속 제조의 기간 동안 비생존가능 세포는 바이러스 복제의 진행 중인/지속하는 기간 및 배양 배지로의 바이러스의 진행 중인/지속하는 방출을 유지시키도록 대체되고, 바이러스 복제 수준이 공정 동안 변동할 수 있더라고, 예를 들어 바이러스 복제는 연속이다(즉, 비중단된다).
일 실시형태에서, 새로 첨가된 세포의 생존능력은 중요한 인자일 수 있고, 따라서 전체 세포 배양물에 걸친 (즉, 생존능력 염색 염료에 의해 시험되는) 세포 생존능력이 측정되는 경우, 이것은 사실 50% 미만일 수 있되, 단 적절한 양의 바이러스의 복제를 유지시키도록 충분한 세포가 존재한다. 그러나, 염료 염색은 비생존가능 세포와 누출된 세포 사이에 반드시 분화할 필요는 없다.
이론에 구속되고자 바라지 않으면서 누출될 수 있는 세포는 또한 바이러스를 생성하도록 생존가능한 것으로 보인다.
몇몇 경우에, 상기 공정에서 사용된 세포의 70% 이상은 예를 들어 본 공정에 의해 이것에 가해진 응력으로 인해 누출될 수 있다. 그러나, 이것은 바이러스를 제조하는 데 있어서 세포의 성능에 영향을 거의 갖지 않는 것으로 보인다. 따라서, 본 공정의 목적을 위해, 세포는 생존능력 염료가 이들을 염색시킬 때에도 바이러스를 제조하기 위해 충분히 생존능력으로 남을 수 있다. 새로운 세포가 배양물에 첨가되는 본 개시내용에 따른 공정에서, 생존능력 염색에 의한 세포 염색은 새로운 세포의 첨가 후에도 진탕 플라스크에서 수행된 실험에서 세포 집단의 70%와 같이 매우 높을 수 있다. 바이오반응기 공정이 포유류 세포에서 바이러스 생성을 수행하기에 특히 적합하고, 바이오반응기 공정으로부터 세포의 염색의 양이 덜할 수 있다는 것이 말해진다.
일 실시형태에서, 본 개시내용에 따른 공정은 바이오반응기에서 수행된다.
적합한 바이오반응기 시스템의 예는 iCELLis 바이오반응기이고, 이것은 예를 들어 마이크로섬유로 충전된 완전 통합된 고밀도 바이오반응기이다. 이 시스템은 부착성 세포를 배양하기에 특히 적합하다. 균등하게 분포된 배지 순환은 빌트인 자기 드라이브 임펠러에 의해 달성되어서, 낮은 전단 응력 및 높은 세포 생존능력을 보장한다. 세포 배양 배지는 바닥으로부터 상부로 고정 층을 통해 흐른다. 상부에서, 배지는 바이오반응기에서 높은 KLa를 유지시키도록 O2를 흡수하는 외부 벽 아래의 박층으로서 떨어진다. 온화한 교반 및 바이오매스 부동화와 함께 이 산소화는 소형의 iCELLis 바이오반응기가 높은 세포 밀도를 달성하고 유지시키도록 하여서, 훨씬 더 큰 교반 탱크 유닛의 생산성에 맞먹는다. 예를 들어 하기에서 추가의 상세내용을 참조한다:
http://www.pall.com/main/biopharmaceuticals/product.page?lid=hw7uq21l
바이러스 복제의 진행 중인 또는 지속하는 기간은 바이러스가 1회 초과의 복제 주기(즉, 2회 이상의 복제 주기를 가짐)에 대한 시간을 가지는 것이다. 복제 주기는 소정의 바이러스가 진입하는 경우 세포가 복제하고 바이러스 및/또는 바이러스 입자는 세포로부터 방출되고, 이후 바이러스 또는 이의 자손은 세포 또는 세포들을 감염시키도록 진행하고 복제하도록 진행하는 것이다.
본 명세서에 사용된 바와 같은, 바이러스의 제조 공정은 바이러스가 복제되고 따라서 바이러스 입자의 수가 증가하는 공정을 의미하도록 의도된다. 특히 제조는 예를 들어 1 내지 9×105개 내지 1 내지 9×1020개의 범위로 치료학적 생성물을 제제화하도록 바이러스 입자의 충분한 수를 제공하는 것이거나, 더 많은 입자가 예컨대 1 내지 9×108개 내지 1 내지 9×1015개의 바이러스 입자의 범위로 생성될 수 있고, 특히 1 내지 9×1010개 또는 1 내지 9×1015개의 바이러스 입자가 10ℓ의 뱃치로부터 생성될 수 있다.
일 실시형태에서, 수율은 1리터당 약 1-2×1014vp이다.
일 실시형태에서, 세포마다 바이러스 제조는, 일 이상의 시점에서 세포마다 55,000; 60,000; 65,000; 70,000; 75,000; 80,000; 90,000; 95,000; 100,000; 105,000; 110,000; 115,000; 120,000; 125,000; 130,000; 135,000; 140,000; 145,000; 150,000; 155,000; 160,000; 165,000; 170,000; 175,000; 176,000; 177,000; 178,000; 179,000; 180,000; 181,000; 182,000; 183,000; 184,000; 185,000; 186,000; 187,000; 188,000; 189,000; 190,000; 191,000; 192,000; 193,000; 194,000; 195,000; 196,000; 197,000; 198,000; 199,000; 200,000; 201,000; 202,000개 이상의 바이러스 입자를 포함하는 군으로부터 예를 들어 독립적으로 선택된, 50,000개 이상의 바이러스 입자이다.
본 명세서에 사용된 바와 같은, 연속 제조의 기간은 단순히 소정의 제조 캠페인의 기간이다. 일반적으로, 본 발명의 공정은 48시간 이상, 예를 들어 70, 80, 84, 86, 90, 96, 100, 108, 120, 124, 128, 132, 144, 156, 168, 180, 192, 204, 216, 228, 240시간 이상(여기서, 상기 값은 ± 3시간임), 예를 들어 144시간 내지 240시간의 범위, 예컨대 156, 168, 180, 192, 204, 216, 228 또는 240시간인 연속 제조의 기간을 가질 것이다.
일 실시형태에서, 배양 기간은 70시간 내지 300시간의 범위, 예를 들어 144 내지 240시간, 예를 들어 144, 156, 168, 180, 192, 204, 216, 228 또는 240시간이다.
일 실시형태에서, 연속 제조 공정은 세포가 100시간 초과 동안 배양된다는 것을 특징으로 한다.
일 실시형태에서, 본 개시내용의 연속 제조 공정은 100시간 이하의 감염 후 배양 기간 및 적어도 하나의 배지 변경 또는 첨가 및 적어도 하나의 세포 변경 또는 세포 첨가를 특징으로 한다.
일 실시형태에서, 연속 제조는 감염 후 35 내지 96시간의 범위이다.
유리하게는, 본 개시내용의 바이러스는 배양 공정이 70시간 이상으로 연장될 때에도 분해되는 것으로 보이지 않는다. 바이러스의 분해는 바이러스의 전염력을 평가함으로써 확인될 수 있다. 바이러스 입자가 분해되면서, 바이러스의 전염력은 감소한다.
본 명세서에 사용된 바와 같은, 최대 전체 바이러스 제조는 세포마다 제조된 바이러스 입자의 전체 수를 의미하고, 상청액 및 세포 중의 바이러스 입자를 포함한다.
일 실시형태에서, 최대 전체 바이러스 제조는 감염 후 약 40 내지 60시간 및 이의 다수, 예를 들어 감염 후 49, 98, 147시간 등에 달성된다.
일 실시형태에서, 최대 전체 바이러스 생성은 감염 후 약 70 내지 90시간 및 이의 다수, 예를 들어 감염 후 140-180시간에 달성된다.
일 실시형태에서, 최대 전체 바이러스 수율은 감염 후 약 60 내지 96시간에 달성된다.
상기 공정에서의 1회 이상의 배지의 변경/대체는 바이러스 수율에 긍정적이고 상당한 영향을 가지는 것으로 보인다. 일 실시형태에서, 배지는 세포를 제거함이 없이 변경된다. 일 실시형태에서, 배지 중 5% 내지 100%는 적어도 1의 시점에서 변경된다. 일 실시형태에서, 배지 및 세포는 제거되고, 예를 들어 (세포 현탁 배양 공정의) 세포 현탁액의 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% 이상은 제거되고, 새로운 배지 및 세포에 의해 대체된다.
일 실시형태에서, 배지는 상기 공정 동안 일 이상의 경우에 첨가된다. 이것은 숙주 세포가 지수 성장기에 있을 때 특히 유리할 수 있다.
일 실시형태에서, 배양 공정은 하나 이상의 배지 변경을 포함한다. 이것은 세포 성장, 수율 또는 유사를 최적화하도록 유리할 수 있다. 배지 변경이 이용되는 경우, 변경되는 배지로부터 바이러스 입자를 회수하는 것이 필요할 수 있다. 이 입자는 바이러스의 수율이 최적화되게 보장하도록 주요 바이러스 뱃치와 조합될 수 있다. 유사한 기법은 바이러스 회수를 최적화하기 위해 관류 공정의 배지와 또한 이용될 수 있다.
일 실시형태에서, 배양 공정은 배지 변경 단계를 포함하지 않는다. 바이러스 입자가 소실되지 않고 따라서 수율이 최적화될 수 있으므로 이것은 유리할 수 있다.
일 실시형태에서, 배지 및/또는 세포는 주기적으로 보충하거나 비축된다.
일 실시형태에서, 세포는 상기 공정 동안, 예를 들어 별개의 시점 또는 시점들에서 또는 연속하여 수확된다.
포유류 세포를 배양하기에 적합한 배지는 시그마-알드리치(Sigma-Aldrich) 사제의 EX-CELL(등록상표) 배지, 예컨대 HEK293 세포에 대한 EX-CELL(등록상표)293 혈청 비함유 배지, CHO 세포에 대한 EX-CELL(등록상표) ACF CHO 배지 혈청 비함유 배지, CHO 세포에 대한 EX-CELL(등록상표) 302 혈청 비함유 배지, 론자(Lonza) 사제 RMPI(예컨대, HEPES 및 L-글루타메이트 함유 RMPI 1640, L-글루타메이트 함유 또는 비함유 RMPI 1640, 및 울트라글루타메이트 함유 RMPI 1640)로부터의 EX-CELL CD 가수분해물 융합 배지 보충물, MEM, DMEM, 및 SFMII 배지를 포함하지만, 이들로 제한되지는 않는다.
일 실시형태에서, 배지는 혈청 비함유이다. 이것은 규제 기관에 의한 제조 공정의 등록을 수월하게 하므로 유리하다.
본 명세서에 사용된 바와 같은, 새로운 배지의 첨가는, 영양소 및 성분이 세포 성장을 지지하기 위해 적합한 수준에 있는, 즉 결핍되지 않는, 배지의 첨가를 의미한다.
본 명세서에 사용된 바와 같은, 배지 변경은 공정에서 사용된 배지 중 일부 또는 전부를 대체하여, 예를 들어 약간의 배지를 제거하고 새로운 배지를 첨가하는 것을 의미한다.
본 명세서에 사용된 바와 같은, "단계 a)로부터 제조된 배지 바이러스로부터의 단리, 여기서 바이러스의 단리는 세포 용해 단계에 후속하지 않는다"는 공정으로부터 얻은 바이러스가 상청액으로부터 단리된 적어도 하나의 단계를 의미한다. 상기 공정은, 적절한 경우, 예를 들어 공정의 종료 시 세포로부터 바이러스를 회수하도록 및/또는 배양물로부터 제거된 세포로부터 바이러스를 회수하도록 세포 용해 단계를 또한 포함할 수 있다.
본 명세서에 사용된 바와 같은, "여기서, 단리는 세포 용해 단계에 후속하지 않는다"는 제조 공정에서 적어도 일 시점에서의 수확이 특정한 용해 단계를 포함하지 않는다는 사실을 의미하도록 의도된다. 즉, 조건이 배양에서 세포의 전부 또는 대부분을 용해시키도록 설계된 단계는 예를 들어 화학 용해 단계, 효소 용해 단계, 용해 완충 단계, 기계적 용해 단계 또는 물리적 용해 단계, 예컨대 원심분리 또는 동결-해동을 이용하지 않는다.
본 명세서에 사용된 바와 같은, 대부분은 매우 다수, 예를 들어 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 또는 99%를 의미한다.
일 실시형태에서, 바이러스가 세포로부터 상청액으로 방출된 후, 이후 수확이 시작하고 그 시점으로부터 앞으로(예를 들어, 순환 배지로부터의 회수에 의해) 연속하여 수행되거나, 별개의 시점, 예를 들어 40 내지 50시간, 60 내지 70시간, 80 내지 100시간, 120 내지 150시간, 160 내지 200시간, 200 내지 250 또는 상기의 조합에서 수확된다. 바이러스의 전부는 아니지만 소정의 양이 상청액으로부터 연속하여 제거되는 조합 및 소정의 시점에서 제거되는 바이러스의 양은 증가하거나 감소한다.
바이러스의 수확은 바이러스 복제를 계속시키도록 배양에서 충분한 바이러스를 남겨야 한다. 이것은 예를 들어 MOI를 모니터링하고 이것을 본 명세서에 기재된 범위 내에 유지시킴으로써 달성될 수 있다.
일 실시형태에서, 모든 바이러스는 공정의 종료 시 일 시점에 수확된다.
일 실시형태에서, 모든 바이러스는 공정의 종료 시 일 시점에 수확되지 않는다.
일 실시형태에서, 본 개시내용의 공정은 하나 이상의 여과 단계를 포함한다. 여과는 세포 및 오염성분을 보유하도록 적절한 바대로 선택될 수 있어서, 바이러스가 필터의 기공을 통해 통과한다. 대안적으로, 여과는 바이러스를 보유하도록 선택되고, 오염성분이 통과하게 허용할 수 있다. 일 실시형태에서, 여과 기법의 조합은 본 개시내용의 공정에서 사용된다.
바이러스는 여과에 의해 상청액으로부터 제거될 수 있고, 예를 들어 바이러스를 통과시키지만 세포 및 다른 세포 배양 성분(오염성분)을 보유하기에 충분한 기공을 가지는 필터를 사용할 수 있다. 일 실시형태에서, 필터는 0.1 내지 10마이크론, 예를 들어 0.2마이크론의 범위로 사용된다. 일 실시형태에서, 점감 여과(graduated filtration)를 사용하고, 예를 들어 10마이크론 필터에 예를 들어 1 내지 5마이크론의 범위의 더 작은 필터가 후행하고, 이것에 결국 예컨대 0.2마이크론 이하의 더 작은 필터가 후행한다.
일 실시형태에서, 정용여과, 예컨대 접선 흐름 여과가 사용된다. 필터의 크기 및 조건은 바이러스를 선택적으로 추출하도록 선택될 수 있다. 적합할 수 있는 필터의 예는 TFF 막 300K NMWC 및 이의 균등물을 포함한다.
일 실시형태에서, 약 300kDa의 컷오프를 가지는 정용여과 시스템은 일반적으로 오염을 제거하면서 바이러스 입자의 90% 이상의 보유를 허용하므로 적절할 수 있다.
내부에 함유된 바이러스의 일부 또는 전부의 추출 후 남은 상청액은, 적절한 경우, 더 많은 바이러스를 추출하고 전체 수율을 증가시키기 위해 추가로 공정처리되거나, 임의로 새로운 배지 또는 이의 성분의 양과 조합되어 배양 시스템으로 재순환되거나, 폐기되거나, 이들의 조합일 수 있다.
일 실시형태에서, (상청액으로부터 제거된 바이러스와 반대로) 세포로부터 단리된 바이러스의 분획(일 분획을 포함)은 상청액으로부터 단리된 바이러스에 대한 첨가 전에 이들에 수행된 적어도 하나의 정제 단계를 가진다.
일 실시형태에서, (상청액으로부터 제거된 바이러스와 반대로) 세포로부터 단리된 바이러스의 분획(일 분획을 포함)은 상청액으로부터 단리된 바이러스에 대한 첨가 전에 이들에 수행된 적어도 하나의 정제 단계를 가지지 않는다.
일 실시형태에서, 90% 초과, 예를 들어 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 100%, 예컨대 95% 이상, 특히 98% 이상의 바이러스는 48시간 시점 및 이의 다수에 상청액에 존재한다.
일 실시형태에서, 상당한 양의 바이러스는 38시간 후 배지에 있다. 바이러스의 예를 들어 50% 초과, 특히 70% 초과는 38시간 후 및 이의 다수, 예를 들어 76, 114, 152, 190, 228 등 후에 배지에 있다.
일 실시형태에서, 바이러스의 50% 이상, 예컨대 55%, 60%, 65%, 70%, 75% 이상은 약 65시간만큼 상청액에 있다.
일 실시형태에서, 바이러스의 70% 이상, 예컨대 75%, 76%, 77%, 78%, 79%, 80% 이상은 약 72시간만큼 상청액에 있다.
일 실시형태에서, 바이러스의 80% 이상, 예컨대 85%, 86%, 87%, 88%, 89%, 80% 이상은 약 96시간만큼 상청액에 있다.
일 실시형태에서, 64시간 시점에, 즉 64시간 후, CVL 펠렛에서 10% 미만의 검출 가능한 바이러스, 예컨대 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%의 검출 가능한 바이러스가 존재한다. 본 명세서에 사용된 바와 같은, CVL는 미정제 바이러스 용해물을 의미한다.
일 실시형태에서, 복제 후 바이러스는 실질적으로 모든 세포에 대해 거의 동시에 세포를 빠져나와서, 상청액 중의 바이러스가 피크인 특정한 시점이 존재한다. 문맥이 달리 표시하지 않은 한, 본 명세서에 사용된 바와 같은, 바이러스 복제 주기는 공정에서의 바이러스 피크를 의미하고, 제1 바이러스 피크는 제1 바이러스 복제 주기의 종료 및 제2 바이러스 복제 주기의 시작 등을 나타낸다. 공정의 이 프로필은 동시에 배양된 세포에 의해 달성될 수 있어서, 이들이 임의의 소정의 시간에 이들의 생활 주기와 동일한 또는 대략 동일한 단계에 있도록 보장한다. 대안적으로, 일련의 상이한 세포는 이 프로필을 평평하게 하고 연속 기준으로 복제된 바이러스를 제공하도록 사용될 수 있다.
배지 또는 상청액에서 바이러스 수준은 HPLC 또는 다른 기법에 의해 모니터링될 수 있다.
일반적으로, 배양물에 첨가된 세포는 배양물에 사용되는 세포의 동일한 유형일 것이다.
배양물에 첨가된 세포는 사전 배양되어서 주요 배양에 대한 첨가에 이들이 더 적합하게 할 수 있어서, 예를 들어 이들이 주요 배양의 세포와 혼합되는 세포 주기 생활 주기의 상용성 단계에 있도록 보장한다.
유리하게는, 생존가능 세포 수준이 증가하는 경우, 바이러스 복제가 바이러스 입자의 절대 수를 증가시키면서, 세포는 바이러스가 감염되게 하도록 이용 가능하여서, 매우 효율적인 공정을 제공한다. 일 실시형태에서, 집단의 다른 특성은 세포를 변경 또는 첨가함으로써 변경되고, 예를 들어 막 투과성이 증가할 수 있다.
일 실시형태에서, 세포는 바이러스의 감염 및 복제 동안 로그 성장기를 지원하고 유지시키도록 확립된 조건 하에 유지된다. 따라서, 일 실시형태에서, 세포 집단의 적어도 부분이 공정에서 임의의 소정의 시간에 로그 성장기에 있고, 예를 들어 세포의 10% 내지 80%, 예컨대 20% 내지 50%는 성장기에 있다. 본 명세서에 사용된 바와 같은, 로그 성장기는 세포가 이용 가능한 영양소, 기질 및 세포에 의해 방출된 생성물에 의한 저해제가 제한 인자가 아닌 성장 배지 조건 하에 지수적으로 증식한다는 것을 의미한다.
세포의 배양은 관류 배양, 유가 배양, 회분 배양, 정상 상태 배양, 연속 배양 또는 기술적으로 적절한 경우 이들 중 하나 이상의 조합, 특히 관류 배양을 이용할 수 있다.
일 실시형태에서, 상기 공정은 관류 공정, 예를 들어 연속 관류 공정이다.
본 명세서에 사용된 바와 같은, "유래한"은 예를 들어 DNA 단편이 아데노바이러스로부터 취해지거나 아데노바이러스에서 원래 발견되는 서열에 상응하는 경우를 의미한다. 이 말은 어떻게 서열이 얻어지는지, 예를 들어 본 개시내용에 따른 바이러스에서 사용된 서열이 합성될 수 있는지를 제한하도록 의도되지 않는다.
일 실시형태에서, 유도체는 원래 DNA 서열과 이의 완전 길이에 걸쳐 100%의 서열 동일성을 가진다.
일 실시형태에서, 유도체는 원래 DNA 서열과 95%, 96%, 97%, 98% 또는 99%의 동일성 또는 유사성을 가진다.
일 실시형태에서, 유도체는 엄격한 조건 하에 원래 DNA 서열에 혼성화한다.
본 명세서에 사용된 바대로, "엄격성"은 통상적으로 대략 Tm(융점)-50℃(프로브의 Tm보다 5℃ 낮음) 내지 Tm보다 약 20℃ 내지 25℃ 낮음의 범위에서 발생한다. 당해 분야의 당업자에 의해 이해되는 것처럼, 엄격한 혼성화는 동일한 폴리뉴클레오타이드 서열을 확인하거나 검출하기 위해 또는 유사한 또는 관련 폴리뉴클레오타이드 서열을 확인하거나 검출하기 위해 사용될 수 있다. 본 명세서에 사용된 바대로, 용어 "엄격한 조건"은 서열 사이에 적어도 95%, 예컨대 적어도 97% 동일성이 존재하는 경우 혼성화가 일반적으로 일어날 것이라는 것을 의미한다.
본 명세서에 사용된 바대로, "혼성화"는, 본 명세서에 사용된 바대로, "폴리뉴클레오타이드 가닥이 염기 쌍 지음을 통해 상보성 가닥과 합해지는 임의의 공정"을 포함해야 한다(Coombs, J., Dictionary of Biotechnology, Stockton Press, New York, N.Y., 1994).
유리하게는, 본 공정은 세포 용해 단계가 회피될 수 있으므로 세포로부터의 오염 DNA 또는 단백질의 더 낮은 시작 농도로 인해 바이러스의 다운스트림 처리를 단순화시킬 수 있다. 다운스트림 처리에서 사용된 시약, 설비 및 시간이 감소할 수 있으므로 이것은 비용을 절약시킬 수 있다. 이것은 또한 오염 DNA의 더 낮은 최종 농도 및/또는 오염 세포 DNA 및 단백질의 큰 단편의 더 낮은 농도와 함께 더 높은 순도를 발생시킬 수 있다.
더욱이, 세포 효소에 대한 바이러스 노출은 가능한 분해물질, 예컨대 세포로부터의 뉴클레아제에 대한 바이러스의 노출을 최소화하는 세포 용해를 피함으로써 최소화된다. 이것은, 예를 들어 전염력에 의해 측정된 바대로, 더 높은 바이러스 안정성 및/또는 효력을 발생시킬 수 있다.
흥미롭게도, 세포를 떠난 후, 본 개시내용의 바이러스는 세포에 부착하지 않고, 그래서 상청액으로부터 용이하게 회수될 수 있다. 이것은 현재의 공정을 수월하게 하는 본 명세서에 기재된 소정의 바이러스의 특징인 현상일 수 있다. 반대로, 야생형 Ad5가 세포에 부착하는 것으로 생각된다. 사실, 결과는 야생형 Ad5, 바이러스 입자가 본 명세서에 기재된 시간에 상청액에 실질적으로 존재하지 않는다는 것을 나타났다.
이론에 구속되고자 바라지 않으면서, 일 실시형태에서, 세포를 떠나고 이것에 부착하지 않는 능력 및/또는 신속한 바이러스 복제 주기는 키메라 E2B 영역 및/또는 E3의 부분의 결실 및/또는 E4 영역의 결실과 연관될 수 있다.
이론에 구속되고자 바라지 않으면서, 일 실시형태에서, 세포를 떠나고 이것에 부착하지 않는 능력 및/또는 신속한 바이러스 복제 주기는 B 그룹 캡시드, 예를 들어 Ad11 캡시드와 연관될 수 있다.
일 실시형태에서, 세포에 대한 부착성의 결여는 종양 바이러스의 헥손 및 섬유와 관련될 수 있고, 예를 들어 이와 관련하여 Ad11과 같은 B 그룹 아데노바이러스로부터의 바이러스 캡시드는 특히 본 개시내용의 복제 가능 바이러스 및 키메라 바이러스에 유리할 수 있다.
본 명세서에 사용된 바와 같은, 신속한 바이러스 복제 주기는 감염 후 50시간 이하의 기간에 바이러스의 적어도 일부가 상청액으로 추출되도록 완전한 주기를 의미한다.
일 실시형태에서, 공지된 표준 시스템은 본 명세서에 기재된 방법에 의해 제조된 바이러스를 처리하기 위해 사용된다.
일 실시형태에서, 예를 들어 연속 공정이 사실 비중단인, 즉 일과 반대로 주 및 개월과 같은 매우 긴 캠페인 동안 실행되는 경우, 배양 용기는 오염성분, 예컨대 DNA 및 세포 부스러기의 축적을 피하기 위해 배양 배지의 인라인 접선 흐름 여과를 제공하도록 채택될 수 있다. 하나의 가능한 배열은 도 2에 도시되어 있고, 여기서 접선 흐름 여과 시스템은 배양 용기에 인접하게 제공된다. 배양 용기와 접선 흐름 시스템 사이의 계면은 도 2에서 편평한 계면으로 도시되어 있다. 그러나, 계면은 임의의 적합한 형상일 수 있고, 예를 들어 접선 흐름 여과는 배양 용기 주위의 재킷으로서 배열될 수 있다.
용기와의 계면은 접선 흐름 필터 막이라 칭해지는 선택적 투과성 막이다. 일 실시형태에서, 막은 300K NMWC TFF이다.
도 2에서의 배열은 오직 적합한 배열의 예이고, 예를 들어 바이러스 추출 후 배지는 배양물로 재순환될 수 있고, 펌프는 다르게 배열될 수 있다.
따라서, 일 실시형태에서, 배양 용기와 연관된 접선 흐름 시스템은 배양물로부터 오염물질을 제거한다. 따라서, 일 실시형태에서, 세포 배양 용기는, 배양물로부터 세포 부스러기 및 오염성분의 제거를 허용하여서, 배양이 유지될 수 있는 기간을 연장하는, 예를 들어 압력차에서 유지되는 접선 흐름 계면을 포함한다.
일 실시형태에서, 배양 용기와 연관된 접선 흐름 시스템은 배양물로부터 바이러스를 제거하고, 따라서 바이러스를 수확하기 위해 사용될 수 있다.
분리 시스템은 바이러스의 1차 회수 또는 2차 회수로서 바이러스를 제거할 수 있다. 분리 시스템은 폐기 출구를 또한 가질 수 있다.
일 실시형태에서, 분리 시스템이 부재할 수 있다.
본 명세서에 사용된 바와 같은, 용기는 예를 들어 배양 백, 팟(pot), 병, 큐브(cube), 관, 바이오반응기 또는 유사에서 세포를 배양하도록 사용하기에 적합한 임의의 용기를 의미한다. 일 실시형태에서, 용기는 가스 투과성 막을 포함할 수 있다.
일 실시형태에서, 배양은 현탁 배양이다. 또 다른 실시형태에서, 배양은 부착성 배양이다.
포유류 세포는 포유류로부터 유래한 세포이다. 일 실시형태에서, 포유류 세포는 HEK, CHO, COS-7, HeLa, Vero, A549, PerC6 및 GMK, 특히 HEK293 또는 A549 세포를 포함하는 군으로부터 선택된다. 일 실시형태에서, 배양물에 첨가된 세포는 빨리 성장하는 세포, 예를 들어 293-F이다. 일 실시형태에서, 사용된 세포/세포주는 캡시드화 세포주, 즉 바이러스 복제를 수월하게 하도록 바이러스 전이유전자를 제공하는 패키징 세포주가 아니다.
예를 들어, 중합체 구(마이크로비드는 또한 마이크로캐리어러 칭해짐)에서 배양되거나 중공 섬유에 부착된 부착성 세포는 교반 탱크 바이오반응기에서 현탁액 중에 사용될 수 있다.
적어도 HEK, CHO 및 A549는 부착성 세포주가 될 수 있다.
본 명세서에 사용된 바와 같은, 포유류 세포의 배양은 세포가 생체외 제어 조건 하에 성장하는 공정을 의미한다. 적합한 조건은 당해 분야에 공지되어 있고, 37℃와 같은 온도를 포함할 수 있다. CO2 수준은 제어될, 예를 들어 10% 미만, 예컨대 5%의 수준에서 유지될 필요가 있을 수 있다. 이의 상세내용은 문헌[Culture of Animal Cells: A Manual of Basic Techniques 및 Specialised Applications Edition Six R. Ian Freshney, Basic Cell Culture (Practical Approach) Second Edition Edited by J.M. Davis]에 기재되어 있다.
보통, 세포는 본 개시내용의 바이러스에 의한 감염 전에 충분한 수를 생성하도록 배양될 것이다. 이 방법은 당해 분야의 당업자에게 공지되어 있거나, 공개 프로토콜 또는 문헌에서 용이하게 이용 가능하다.
일반적으로, 세포는 상업용 규모, 예를 들어 5ℓ, 10ℓ, 15ℓ, 20ℓ, 25ℓ, 30ℓ, 35ℓ, 40ℓ, 45ℓ, 50ℓ, 100ℓ, 200ℓ, 300ℓ, 400ℓ, 500ℓ, 600ℓ, 700ℓ, 800ℓ, 900ℓ, 1000ℓ 또는 유사로 배양될 것이다. 일 실시형태에서, 본 개시내용에 따른 연속 공정의 스케일은 150ℓ 이하이다.
본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스 및/또는 B 그룹 복제 가능 아데노바이러스는 Ad5와 같은 벡터로서 사용된 아데노바이러스의 특성과 다른 특성을 가지고, 이것은 배양의 비교적 짧은 기간에 걸쳐 세포 용해에 대한 수요 없이 이들이 배지로부터 회수될 수 있다는 사실을 포함한다. 오랜 기간 Ad5(그룹 C 바이러스)의 E3 영역에서 암호화된 아데노바이러스 사멸 단백질이 바이러스가 세포를 떠나는 데 필요하다고 생각되므로, 이것은 당해 분야에서 예상된 것과 반대이다. 따라서, 이론에 구속되고자 바라지 않으면서, B 그룹 바이러스는 세포를 떠나는 기전을 가지는 것으로 보인다.
더욱이, 본 개시내용의 바이러스, 예컨대 키메라 종양 아데노바이러스는 세포를 떠난 후 세포와 회합하거나 이에 부착하는 것으로 보이지 않아서, 특히 세포 배양 조건이 최적화될 때, 상청액으로부터 회수를 또한 촉진한다.
일 실시형태에서, 상기 공정은 약 40℃ 미만, 예를 들어 약 35 내지 39℃, 예컨대 37℃의 온도에서 수행된다.
일 실시형태에서, 상기 공정은 10% 미만, 예를 들어 약 4-6%의 CO2, 예컨대 5% CO2의 이산화탄소 농도에 의해 수행된다.
일 실시형태에서, 상기 공정은 6 내지 8의 범위의 pH에서, 예컨대 pH 7.4에서 수행된다.
일 실시형태에서, 바이러스, 예컨대 키메라 종양 바이러스 입자를 함유하는 배지는 세포를 제거하고 추가의 다운스트림 처리에 대한 미정제 상청액을 제공하기 위해 여과된다.
일 실시형태에서, 접선 흐름 필터가 사용된다.
일 실시형태에서, 배지는 셀룰로스 기반 뎁스 필터를 가지는 밀리포어 Millistak+(등록상표) POD 시스템을 사용하여 여과된다. Millistak+(등록상표) 뎁스 필터 배지는 포드 필터 시스템(Pod Filter System)인 확장 가능한, 일회용 포맷으로 제공된다. 이것은 세포 배양을 포함하는 매우 다양한 1차 및 2차 청징 분야에 이상적이다.
Millistak+(등록상표) 포드 필터는 특정한 적용 수요를 맞추기 위해 3개의 구별되는 일련의 배지 등급에서 이용 가능하다. Millistak+(등록상표) DE, CE 및 HC 배지는 구배 밀도 매트릭스를 통해 최적 성능, 및 양성 표면 전하 특성을 전달한다. 일 실시형태에서, 여과는 접선 흐름 기술을 이용하여, 예를 들어 펠리콘 미니(Pellicon Mini) 카세트 막 홀더, 압력 센서, 혼합기를 가지는 10리터 리사이클 탱크, 보유액 유량계, 저울, 공급 펌프, 이송 펌프, 배관 및 밸브를 포함하는 Cogent(상표명) M 시스템을 이용하여 수행된다. 시스템의 제어 및 조작은 반자동 정용여과/농축을 제외하고 수동이다. 오퍼레이터는 펌프 속도, 모든 밸브 및 조작 절차의 수동 제어를 가진다. 바이러스는, 원하는 경우, 이 단계에서 최종 완충제로 또한 제제화될 수 있다.
일 실시형태에서, 다운스트림 처리는 8㎛ 캡슐 필터(Sartopure PP2 8㎛), 이어서 직렬의 3.0㎛/0.8㎛ 캡슐 필터(Sartoclean CA, 3.0㎛/0.8㎛, 0.2㎡)로 이루어진 청징 어셈블리로 이루어진다.
따라서, 일 실시형태에서, 여과 단계에서, 농축되고 컨디셔닝된 아데노바이러스 재료는 최종 또는 거의 최종 제제에 제공된다.
일 실시형태에서, 상기 공정은 2개 이상의 여과 단계를 포함한다.
일 실시형태에서, 다운스트림 처리는 Millistak + 포드 시스템 35 CE 및 50 CE 카세트, 이어서 직렬의 옵티캡(opticap) XL 10 익스프레스 0.5/0.2㎛ 막 필터를 포함한다.
일 실시형태에서, 상기 공정은 CsCl 구배, 크로마토그래피 단계, 예컨대 크기 배제 크로마토그래피, 이온 교환 크로마토그래피, 특히 음이온 교환 크로마토그래피, 및 이들의 조합으로부터 선택된 정제 단계를 추가로 포함한다.
이온 교환 크로마토그래피는 DNA에 매우 강하게 결합하고, 통상적으로 임의의 잔류 DNA가 제거되는 장소이다. 이온 교환 수지/막은 바이러스 및 DNA 둘 다에 결합하고, 염 구배 용리 동안 바이러스는 보통 처음에 칼럼을 용리시키고(저염 구배), DNA와 수지와의 상호작용이 바이러스보다 강하므로 DNA는 훨씬 더 높은 염 농도에서 용리된다.
일 실시형태에서, 크로마토그래피 단계 또는 단계들은 예를 들어 비아 세퍼레이션스(BIA Separations)사로부터 이용 가능한 모노리쓰 기술을 이용한다,
일 실시형태에서, Sartobind Q(4차 아민 막 정제 공정)는 정제 단계로서 사용된다.
일 실시형태에서, Source Q 수지는 정제 단계에서 사용된다.
일 실시형태에서, Sartobind Q가 사용되고, 단리된 바이러스의 다운스트림 처리에서 Source Q 수지가 후행한다.
일 실시형태에서, Sartobind Q가 사용되고, 단리된 바이러스의 다운스트림 처리에서 CIM(등록상표) 모노리쓰 칼럼(CIM-QA; 4차 아민 막 정제 공정)이 후행한다.
일 실시형태에서, Source Q가 정제 단계에서 사용된다.
제조 공정(즉, 포획 및 연마 단계)의 크로마토그래피 단계 동안, 몇몇 칼럼은 직렬로 연결될 수 있어서, 제1 칼럼으로부터 누출된 생성물이 직렬의 제2 칼럼에 포획되므로, 제1 칼럼은 재료 손실 없이 결합 능력을 넘어 과로딩될 수 있다. 연속 다중칼럼 제조 공정의 원칙은 따라서 항류 크로마토그래피 공정이라 칭해지는 생성물 흐름과 반대 방향의 칼럼의 (모의) 이동을 생성한다.
3개 이상의 칼럼은 항류 크로마토그래피 공정에 필요할 수 있어서, (결합 능력을 넘어 과로딩된) 제1 칼럼은 세척 단계, 용리, 재생 및 재평형 단계에 걸쳐 처리되고 이후 생성물을 포획하도록 라인에 다시 놓이도록 충분한 시간을 가져야 하고, 직렬의 또 다른(생성물 포화) 칼럼은 상기 기재된 재평형 단계로 세척을 겪는다. 칼럼은 보통 크로마토그래피 배지의 일생 동안 각각의 정제 실행에 걸쳐 수회 순환된다.
일 실시형태에서, 정제 후 제조된 바이러스는 80ng/㎖ 미만, 예를 들어 60ng/㎖ 내지 10ng/㎖의 오염 DNA를 함유한다.
일 실시형태에서, 실질적으로 모든 오염 DNA 단편은 700개 이하, 예를 들어 500bp 이하, 예컨대 200bp 이하의 염기 쌍이다.
일 실시형태에서, 정제된 바이러스 생성물에서의 잔류 숙주 세포 단백질 함량은, 특히 ELISA 검정에 의해 측정될 때, 20ng/㎖ 이하, 예를 들어 15ng/㎖ 이하이다.
일 실시형태에서, 정제된 바이러스 생성물에서의 잔류 트윈(tween)은 0.1mg/㎖ 이하, 예컨대 0.05mg/㎖ 이하이다.
100U/㎖의 벤조나제(Merck)는 숙주 세포 DNA를 분해시키도록 사용된다. 벤조나제 처리는 +37℃에서 30분 동안 수행된다. 벤조나제는 실온에서 1시간 동안 고염 항온처리에 의해 중지된다. 세포 DNA를 분해시키는 벤조나제의 사용은 또한 회피되거나 원하는 경우 감소할 수 있어서, 유리할 수 있다. 특히, 벤조나제의 제거 및 잔류 벤조나제의 부재를 나타내는 시험이 회피될 수 있다. 일 실시형태에서, 벤조나제는 본 공정에서 사용되지 않는다.
일 실시형태에서, 정제된 바이러스 생성물에서 잔류 벤조나제 함량은 1ng/㎖ 이하, 예컨대 0.5ng/㎖ 이하이다.
본원의 맥락에서, 배지 및 배지들은 상호 교환되어 사용될 수 있다.
종양 바이러스는 암 세포를 우선적으로 감염시키고, 예를 들어 이의 용해에 의해 세포 사멸을 재촉하거나, 암 세포에서 선택적으로 복제하는 것이다.
암 세포를 우선적으로 감염시키는 바이러스는 정상 건강한 세포와 비교하여 암 세포를 감염시킬 더 높은 속도를 나타내는 바이러스이다.
본 개시내용의 키메라 아데노바이러스는 일련의 종양 세포에서 이의 용해 가능성을 검사함으로써 특이적 종양 타입에 대한 이의 선호도에 대해 평가될 수 있고, 예를 들어 결장 종양 세포주는 HT-29, DLD-1, LS174T, LS1034, SW403, HCT116, SW48, 및 Colo320DM을 포함한다. 임의의 이용 가능한 결장 종양 세포주는 이러한 평가를 위해 사용될 수 있다.
전립선 세포주는 DU145 및 PC-3 세포를 포함한다. 췌장 세포주는 Panc-1 세포를 포함한다. 유방 종양 세포주는 MDA231 세포주를 포함하고, 난소 세포주는 OVCAR-3 세포주를 포함한다. 폐암 세포주는 A549를 포함한다. 조혈 세포주는 라지(Raji) 및 다우디(Daudi) B 림프구성 세포, K562 적혈아구성 세포, U937 골수성 세포 및 HSB2 T-림프구성 세포를 포함하지만, 이들로 제한되지는 않는다. 다른 이용 가능한 종양 세포주가 동등하게 사용될 수 있다.
비키메라인 것, 예를 들어 Ad11, 예컨대 Ad11p를 포함하는 종양 바이러스는 이 세포주에서 유사하게 평가될 수 있다.
일 실시형태에서, 키메라 종양 바이러스는 프로그래밍된 세포 사멸을 재촉하는 아폽토시스성이다.
일 실시형태에서, 키메라 종양 바이러스는 세포용해성이다. 본 개시내용의 키메라 종양 아데노바이러스의 세포용해 활성은 대표적인 종양 세포주에서 결정될 수 있고, 데이터는, 표준(즉, 1의 효력을 고려)으로 사용되는, 예를 들어 C 하위군에 속하는 아데노바이러스, 바람직하게는 Ad5에 의한 효력의 측정으로 변환된다. 세포용해 활성을 결정하기 위한 적합한 방법은 MTS 검정이다(WO2005/118825(본 명세서에 참고로 포함됨)의 실시예 4, 도 2 참조).
일 실시형태에서, 본 개시내용의 키메라 종양 아데노바이러스는 세포 괴사를 발생시킨다.
일 실시형태에서, 키메라 종양 바이러스는 암 세포에 대한 증대 치료 지수를 가진다.
"치료 지수" 또는 "치료 창"은, 정상(즉, 비암성) 세포주에서 동일한 아데노바이러스의 효력에 의해 관련 암 세포주에서의 키메라 종양 아데노바이러스의 효력을 나눔으로써 결정될 수 있는, 소정의 아데노바이러스의 종양 가능성을 나타내는 숫자이다.
일 실시형태에서, 키메라 종양 바이러스는 결장암 세포, 유방암 세포, 두경부암, 췌장암 세포, 난소암 세포, 조혈 종양 세포, 백혈병 세포, 신경교종 세포, 전립선암 세포, 폐암 세포, 흑색종 세포, 육종 세포, 간암 세포, 신장암 세포, 방광암 세포 및 전이성 암 세포를 포함하는 군으로부터 선택된 하나 이상의 암 세포에서 증대 치료 지수를 가진다.
본 명세서에 사용된 바와 같은, 키메라 종양 아데노바이러스는 적어도 2개의 구별되는 아데노바이러스 혈청형으로부터 유래한 DNA 서열을 가지고 바이러스가 종양인 E2B 영역을 포함하는 아데노바이러스를 의미한다.
현재 약 56개의 아데노바이러스 혈청형이 존재한다. 표 1은 아데노바이러스 혈청형의 분할을 보여준다:
하위군 아데노바이러스 혈청형
A 12, 18, 31
B 3, 7, 11, 14, 16, 21, 34, 35, 50, 55
C 1, 2, 5, 6
D 8-10, 13, 15, 17, 19, 20, 22-30, 32, 33, 36-39,
42-51, 53, 54, 56
E 4
F 40, 41
G 52
E2B 영역은 아데노바이러스에서 공지된 영역이고, 바이러스 게놈의 약 18%를 나타낸다. 이것은 단백질 IVa2, DNA 중합효소 및 말단 단백질을 암호화하는 것으로 생각된다. Ad11(Ad11p라 칭해짐)의 슬로비트스키 균주에서, 이 단백질은 게놈 서열에서 각각 위치 5588-3964, 8435-5067 및 10342-8438에서 암호화되고, E2B 영역은 10342-3950에 이른다. E2B 영역의 정확한 위치는 다른 혈청형에서 변할 수 있지만, 이들이 동일한 일반 조직화를 모두 가지면서 기능은 모든 인간 아데노바이러스 게놈에서 보존된다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 B 하위군 헥손을 가진다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 Ad11 헥손, 예컨대 A11p 헥손을 가진다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 B 하위군 섬유를 가진다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 Ad11 섬유, 예컨대 A11p 섬유를 가진다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 동일한 혈청형, 예를 들어 B 하위군 아데노바이러스, 예컨대 Ad11, 특히 Ad11p로부터의 섬유 및 헥손 단백질을 가진다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 키메라 종양 바이러스는 동일한 혈청형, 예를 들어 Ad11, 특히 Ad11p로부터의 섬유, 헥손 및 펜톤 단백질(예를 들어, 후자의 게놈 서열의 위치 30811-31788, 18254-21100 및 13682-15367에서 발견됨)을 가진다.
제1 바이러스에 대한 명확한 혈청형의 바이러스는 동일한 하위군 또는 상이한 하위군 유래일 수 있지만, 항상 상이한 혈청형 유래는 아닐 것이다. 일 실시형태에서, 조합은 (제1 Ad 혈청형: 제2 Ad 혈청형)에서 하기와 같다: AA, AB, AC, AD, AE, AF, AG, BB, BC, BD, BF, BG, CC, CD, CE, CF, CG, DD, DE, DF, DG, EE, EF, EG, FF, FG 및 GG.
일 실시형태에서, 키메라 E2B 영역은 Ad3 및 Ad11(특히 Ad11p)로부터 유래한다.
일 실시형태에서, E2B 영역은 본 명세서에서 서열 번호 47에 기재된 서열이다.
일 실시형태에서, 바이러스는 B 그룹 아데노바이러스, 예를 들어 Ad11로부터의 헥손 및 섬유를 가지고, 특히 바이러스는 ColoAd1이다.
일 실시형태에서, 단리된 정제된 EnAd가 제공되고, 여기서 오염 DNA 함량은 80ng/㎖ 미만이다.
EnAd는 WO2005/118825에 개시되어 있고, 바이러스에 대한 완전 서열, 즉 서열 번호 12는 본 명세서에 제공된다.
대안적인 키메라 종양 바이러스는 각각 WO 2008/080003에 개시되고 본 명세서에 참고로 포함된 서열 번호 2 및 3인 OvAd1 및 OvAd2를 포함한다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 본 개시내용의 키메라 종양 바이러스는 전이유전자로의 1개 이상의 제한 부위를 포함한다. 일 실시형태에서, 제한 부위는 후기 유전자, 예를 들어 L5에 있거나, 이에 인접하다.
일 실시형태에서, 본 개시내용의 바이러스, 예컨대 본 개시내용의 키메라 종양 바이러스는 1개 이상의 전이유전자, 예를 들어 치료학적 펩타이드(들) 또는 단백질 서열(들)을 암호화하는 1개 이상의 전이유전자를 포함한다.
일 실시형태에서, 키메라 종양 바이러스는 적어도 1개의 전이유전자를 암호화한다. 적합한 전이유전자는 소위 자살 유전자, 예컨대 p53; 사이토카인, 예컨대 IL-2, IL-6, IL-7, IL-12, IL-15, IL-18, IL-21, GM-CSF 또는 G-CSF, 인터페론(예를 들어, I형 인터페론, 예컨대 IFN-알파 또는 베타, II형 인터페론, 예컨대 IFN-감마), TNF(예를 들어, TNF-알파 또는 TNF-베타), TGF-베타, CD22, CD27, CD30, CD40, CD120을 암호화하는 폴리뉴클레오타이드 서열; 단일클론 항체를 암호화하는 폴리뉴클레오타이드, 예를 들어 트라스투주맙, 세툭시맙, 파니투맙, 페르투주맙, 에프라투주맙, 항-EGF 항체, 항-VEGF 항체 및 항-PDGF 항체, 항-FGF 항체, 관문 저해제 항체, 예컨대 항-CTLA4, 항-PD1 및 항-PDL1 항체, 또는 이의 표적 항원 결합 단편, 또는 종양 연관 항원, 예컨대 NY-ESO1, WT1, MAGE-A3 및 당해 분야에 공지된 기타를 포함한다.
그 자체가 종양 또는 면역 반응을 조절하도록 작용하고, 치료학적으로 작용하는 분자를 암호화하거나, 이러한 분자의 활성을 직접적으로 또는 간접적으로 저해하거나, 활성화하거나 증대시키는 물질인, 일련의 상이한 유형의 전이유전자, 및 이들의 조합이 고안된다. 이러한 분자는 단백질 리간드 또는 리간드의 활성 결합 단편, 항체(전장 또는 단편, 예컨대 Fv, ScFv, Fab, F(ab)'2 또는 더 작은 특이적 결합 단편), 또는 다른 표적 특이적 결합 단백질 또는 펩타이드(예를 들어, 파지 디스플레이 등과 같은 기법에 의해 선택될 수 있음), 천연 또는 합성 결합 수용체, 리간드 또는 단편, 표적(예를 들어, siRNA 또는 shRNA 분자, 전사 인자)을 암호화하는 유전자의 전사 또는 번역을 조절하는 특이적 분자를 포함한다. 분자는, 항원 제시 세포, 예컨대 수지상 세포(예를 들어, 항-DEC-205, 항-만노스 수용체, 항-덱틴)에 특이성을 가지는 항체 또는 항체 단편에 융합된, 이의 활성, 안정성, 특이성 등을 증대시키기 위해 다른 펩타이드 서열과의 융합 단백질의 형태일 수 있다(예를 들어, 리간드는 이합체를 형성하고 안정성을 증대시키기 위해 면역글로불린 Fc 영역과 융합될 수 있다). 전이유전자는 예를 들어 "삽입체 보유 아데노바이러스"에 의해 감염된 세포의 검출, 종양 또는 배농 림프관 및 림프절의 영상화 등에 사용될 수 있는 리포터 유전자를 또한 암호화할 수 있다.
일 실시형태에서, 키메라 종양 바이러스에 의해 감염된 암 세포는 용해되어서, 전이유전자에 의해 암호화된 단백질을 포함할 수 있는 세포의 내용물을 방출한다.
일 실시형태에서, 세포에서 복제된 전체 바이러스 중 40% 내지 93% 이상은 배지로부터 회수 가능하고, 예를 들어 전체 바이러스 중 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 또는 92%가 회수 가능하고, 예컨대 전체 바이러스 중 94, 95, 96, 97, 98, 99 또는 100%가 회수 가능하다.
일 실시형태에서, 상기 공정은 GMP 제조 공정, 예컨대 cGMP 제조 공정이다.
일 실시형태에서, 상기 공정은 저장에 적합한 완충제 중에 바이러스를 제제화하는 단계를 추가로 포함한다.
일 실시형태에서, 형성은 실온에서의 저장에 적합하다.
일 실시형태에서, 형성은 4℃ 이하에서의 저장에 적합하다.
일 실시형태에서, 형성은 동결된다.
일 실시형태에서, 본 개시내용은 본 방법으로부터 얻어지거나 얻어질 수 있는 바이러스 또는 바이러스 제제로 확장된다.
본 명세서의 맥락에서, "포함하는"은 "함유하는" 것으로 해석되어어야 한다.
소정의 구성요소를 포함하는 본 발명의 양태는 또한 대안적인 실시형태 관련 구성요소로 "이루어진" 또는 "본질적으로 이루어진"으로 확장되는 것으로 의도된다.
기술적으로 적절한 경우, 본 발명의 실시형태는 본 개시내용의 상이한 독립적인 양태를 언급하더라도 조합될 수 있는데, 왜냐하면 본 명세서에서 모든 본 발명에 기초하는 기본 기술이 동일하기 때문이다.
실시형태는 소정의 특징/구성요소를 포함하는 것으로 본 명세서에 기재되어 있다. 본 개시내용은 또한 상기 특징/구성요소를 구성하는 또는 본질적으로 구성하는 별개의 실시형태로 연장된다.
기술 참고문헌, 예컨대 특허 및 출원은 본 명세서에 참고로 포함된다.
본 명세서에서 구체적으로 및 명확히 언급된 임의의 실시형태는 단독으로 또는 하나 이상의 추가의 실시형태와 조합되어 부인의 기초를 형성할 수 있다.
본 발명은 첨부한 도면을 참조하여 오직 하기 예에서 예시로서 추가로 기재되어 있다.
문단
1. 복제 가능 아데노바이러스; 또는
E2B 영역을 포함하는 게놈을 가지는 복제 가능 또는 결핍 키메라 종양 아데노바이러스(여기서, 상기 E2B 영역은 제1 아데노바이러스 혈청형으로부터 유래한 핵산 서열 및 제2의 구별되는 아데노바이러스 혈청형으로부터 유래한 핵산 서열을 포함하고; 상기 제1 혈청형 및 제2 혈청형은 아데노바이러스 B, C, D, E 또는 F 하위군으로부터 각각 선택됨)의 제조를 위한 연속 공정으로서, 상기 공정은
A) 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
B) 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지되는, 연속 공정
또는
1. B형 아데노바이러스의 제조를 위한 연속 공정으로서, 상기 공정은
C) 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있음), 및
D) 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계(여기서, 바이러스의 단리는 세포 용해 단계에 후속하지 않음)를 포함하고,
바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 바이러스를 복제하기에 적합한 수준으로 용기 내에 유지되는, 공정.
2. 문단 1에 있어서, 바이러스는 B 그룹 아데노바이러스, 예를 들어 Ad11로부터의 헥손 및 섬유를 가지고, 특히 바이러스는 EnAd인, 공정.
3. 문단 1 또는 2에 있어서, 바이러스는 복제 가능한, 공정.
4. 문단 1 내지 3 중 어느 하나에 있어서, 연속 제조 기간은 적어도 2회의 바이러스 복제 주기를 포함하는, 공정.
5. 문단 4에 있어서, 각각의 바이러스 복제 주기는 70시간 내지 300시간의 범위인, 공정.
6. 문단 1 내지 4 중 어느 하나에 있어서, 바이러스 감염 및 제조를 위한 생존가능 세포는 배양을 증대시키도록 추가의 세포의 첨가에 의해 바이러스를 복제하기에 적합한 수준으로 상기 용기 내에서 유지되는, 공정.
7. 문단 1 내지 6 중 어느 하나에 있어서, 포유류 세포는 HEK, CHO, HeLa, Vero, PerC6 및 GMK, 특히 HEK293을 포함하는 군으로부터 선택되는, 공정.
8. 문단 1 내지 7 중 어느 하나에 있어서, 배양은 5ℓ의 이상의 규모인, 공정.
9. 문단 1 내지 8 중 어느 하나에 있어서, 배양 동안 바이러스는 40 내지 150ppc, 예컨대 50 내지 100ppc의 범위의 농도인, 공정.
10. 문단 1 내지 9 중 어느 하나에 있어서, 세포는 바이러스 of 1 내지 9×104vp/㎖ 이상, 예컨대 1 내지 9×105, 1 내지 9×106, 1 내지 9×107, 1 내지 9×108, 1 내지 9×109, 특히 4 내지 5×106vp/㎖의 바이러스의 시작 농도에 의해 감염된, 공정.
11. 문단 1 내지 10 중 어느 하나에 있어서, 종양 바이러스의 분획을 제공하고, 상기 공정은 상이한 공정의 것에 의해 만들어진 종양 바이러스의 제2 분획 또는 분획들이 제1 분획과 조합되도록 추가의 단계를 포함하는, 공정.
12. 문단 1 내지 11 중 어느 하나에 있어서, 관류 배양이 이용되는, 공정.
13. 문단 1 내지 12 중 어느 하나에 있어서, 현탁 배양이 이용되는, 공정.
14. 문단 1 내지 12 중 어느 하나에 있어서, 부착 배양이 이용되는, 공정.
15. 문단 1 내지 14 중 어느 하나에 있어서, 공정은 GMP 제조 공정인, 공정.
16. 문단 1 내지 15 중 어느 하나에 있어서, 필터는 접선 필터인, 공정.
17. 문단 1 내지 16 중 어느 하나에 있어서, 공정은 CsCl 구배, 크로마토그래피 단계, 예컨대 이온 교환 크로마토그래피, 특히 음이온 교환 크로마토그래피, 및 이들의 조합으로부터 선택된 정제 단계를 추가로 포함하는, 공정.
18. 문단 1 내지 17 중 어느 하나에 있어서, 전체 바이러스 중 40% 내지 93%는 배지로부터 회수 가능한, 공정.
19. 아데노바이러스의 제조 공정으로서,
a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 아데노바이러스에 의해 감염된 포유류 세포를 배양하는 단계(여기서, 세포는 바이러스 복제를 지지할 수 있고, 바이러스의 시작 시드 밀도는 1 내지 2×106vp/㎖(예컨대, 1×106vp/㎖)의 범위이고, 감염 다중도는 5 내지 20, 예컨대 10 내지 15, 특히 12.5vp/세포의 범위임), 및
b. 세포로부터 바이러스를 수확하기 위해 바이러스 감염 후 24시간 내지 75시간의 기간에 용해 단계를 수행하는 단계(여기서, 예를 들어 상기 용해 단계는 감염 후 65시간 내지 70시간에, 예컨대 감염 후 66시간, 67시간, 68시간 또는 69시간에 수행됨)를 포함하는, 아데노바이러스의 제조 공정.
20. 문단 1 내지 19 중 어느 하나에 있어서, 저장에 적합한 완충제 중에 바이러스를 제제화하는 단계를 추가로 포함하는, 공정.
21. 예를 들어 제1항 내지 제20항 중 어느 한 항에 본 명세서에 기재된 이 공정으로부터 얻어지거나 얻어질 수 있는 바이러스 또는 제제.
서열
서열 번호 1 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-77 바이러스 게놈 서열. 전이유전자 카세트는 5' 분지된 스플라이스 억셉터 서열(bSA), 5' 리더를 가지는 ab 중쇄 서열, IRES, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 2 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-135 바이러스 게놈 서열. 전이유전자 카세트는 5' 짧은 스플라이스 억셉터 서열(short splice acceptor sequence; SSA), 5' 리더를 가지는 ab 중쇄 서열, IRES, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 3 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 포함하는 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더를 가지는 ab 중쇄 서열, SSA 및 5' 리더를 가지는 ab 경쇄 서열을 함유한다.
서열 번호 4 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 포함하는 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더를 가지는 ab 중쇄 서열, SSA, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 5 영역 BY에서 삽입된 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 포함하는 NG-74 바이러스 게놈 서열. 전이유전자 카세트는 bSA, 5' 리더를 가지는 항-VEGF ScFv 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 6 영역 BY에서 삽입된 C 말단 His6 태그를 가지는 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-78 바이러스 게놈 서열. 전이유전자 카세트는 bSA, 5' 리더를 가지는 항-VEGF ScFv 서열 및 3' 6 x 히스티딘 서열 및 폴리(A) 서열을 함유한다.
서열 번호 7 영역 BY에서 삽입된 C 말단 His6 태그를 가지는 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-76 바이러스 게놈 서열. 전이유전자 카세트는 CMV 프로모터, 5' 리더를 가지는 항-VEGF ScFv 서열 및 3' 6 x 히스티딘 서열 및 폴리(A) 서열을 함유한다.
서열 번호 8 영역 BY에서 삽입된 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-73 바이러스 게놈 서열. 전이유전자 카세트는 CMV 프로모터, 5' 리더를 가지는 항-VEGF ScFv 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 9 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-134 바이러스 게놈 서열. 전이유전자 카세트는 CMV 프로모터, 5' 리더를 가지는 ab 중쇄 서열, IRES, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 10 EnAd 게놈의 bp 28166-28366에 상응하고 이를 포함하는 BX DNA 서열.
서열 번호 11 EnAd 게놈의 bp 29345-29379에 상응하고 이를 포함하는 BY DNA 서열.
서열 번호 12 EnAd 게놈.
서열 번호 13 CMV 외인성 프로모터.
서열 번호 14 PGK 외인성 프로모터.
서열 번호 15 CBA 외인성 프로모터.
서열 번호 16 짧은 스플라이스 억셉터(SSA). 제로 서열
서열 번호 17 스플라이스 억셉터(SA).
서열 번호 18 분지된 스플라이스 억셉터(bSA).
서열 번호 19 내부 리보솜 유입 서열(IRES).
서열 번호 20 폴리아데닐화 서열.
서열 번호 21 리더 서열(HuVH).
서열 번호 22 리더 서열(HG3).
서열 번호 23 히스티딘 태그.
서열 번호 24 V5 태그.
서열 번호 25 P2A 펩타이드.
서열 번호 26 F2A 펩타이드.
서열 번호 27 E2A 펩타이드.
서열 번호 28 T2A 펩타이드.
서열 번호 29 항-VEGF ab VH 사슬 아미노산 서열.
서열 번호 30 항-PD-L1 항체 VH 사슬 아미노산 서열.
서열 번호 31 항-VEGF ab VL 사슬 아미노산 서열.
서열 번호 32 항-PD-L1 항체 VL 사슬 아미노산 서열.
서열 번호 33 인간 IgG1 불변 중쇄 아미노산 서열.
서열 번호 34 인간 IgG1 변형된 불변 중쇄 아미노산 서열.
서열 번호 35 인간 카파 불변 경쇄 아미노산 서열.
서열 번호 36 항-VEGF ScFv 아미노산 서열.
서열 번호 37 항-PD-L1 ScFv 아미노산 서열.
서열 번호 38 녹색 형광 단백질 아미노산 서열.
서열 번호 39 루시퍼라제 아미노산 서열.
서열 번호 40 인간 종양 괴사 인자 알파(TNFα) 아미노산 서열.
서열 번호 41 인간 인터페론 감마(IFNγ) 아미노산 서열.
서열 번호 42 인간 인터페론 알파 (IFNα) 아미노산 서열.
서열 번호 43 인간 암/고환 항원 1(NY-ESO-1) 아미노산 서열.
서열 번호 44 인간 MUC-1 아미노산 서열.
서열 번호 45 코작 서열. gccaccatg(제로 서열)
서열 번호 46 영역 BY에서 삽입된 항-PD-L1 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-177 바이러스 게놈 서열. 전이유전자 카세트는 CMV 프로모터, 5' 리더를 가지는 ab 중쇄 서열, IRES, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 47 EnAd 게놈(bp 10355-5068)의 E2B 영역에 상응하는 DNA 서열.
서열 번호 48 영역 BY에서 삽입된 C 말단 His6 태그를 가지는 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-167 바이러스 게놈 서열. 전이유전자 카세트는 5' SSA, 5' 리더를 가지는 항-VEGF ScFv 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 49 영역 BY에서 삽입된 사이토카인, IFNγ를 암호화하는 전이유전자 카세트를 포함하는 NG-95 바이러스 게놈 서열. 전이유전자 카세트는 5' CMV 프로모터, IFNγ cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 50 영역 BY에서 삽입된 사이토카인, IFNα를 암호화하는 전이유전자 카세트를 포함하는 NG-97 바이러스 게놈 서열. 전이유전자 카세트는 5' CMV 프로모터, IFNγ cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 51 영역 BY에서 삽입된 사이토카인, IFNγ를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-92 바이러스 게놈 서열. 전이유전자 카세트는 5' bSA, IFNγ cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 52 영역 BY에서 삽입된 사이토카인, IFNα를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-96 바이러스 게놈 서열. 전이유전자 카세트는 5' bSA, IFNγ cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 53 영역 BY에서 삽입된 사이토카인, IFNα를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-139 바이러스 게놈 서열. 전이유전자 카세트는 5' SSA, IFNα cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 54 제한 부위 삽입체(BY).
서열 번호 55 제한 부위 삽입체(BX).
서열 번호 56 영역 BY에서 삽입된 종양 연관 항원, NY-ESO-1을 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-220 바이러스 게놈 서열. 전이유전자 카세트는 5' PGK 프로모터, NY-ESO-1 cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 57 영역 BY에서 삽입된 종양 연관 항원, NY-ESO-1을 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-217 바이러스 게놈 서열. 전이유전자 카세트는 5' CMV 프로모터, NY-ESO-1 cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 58 영역 BY에서 삽입된 항-CTLA-4 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-242 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더를 가지는 ab 중쇄 서열, IRES, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 59 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-165 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더를 가지는 ab 중쇄 서열, P2A 펩타이드 서열, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 60 영역 BY에서 삽입된 항-PD-L1 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-190 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더를 가지는 ab 중쇄 서열, P2A 펩타이드 서열, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 61 영역 BY에서 삽입된 C 말단 His6 태그를 가지는 항-PD-L1 ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-221 바이러스 게놈 서열. 전이유전자 카세트는 5' SSA, 5' 리더를 가지는 항-PD-L1 ScFv 서열 및 3' 6 x 히스티딘 서열, 이어서 폴리(A) 서열을 함유한다.
서열 번호 62 영역 BY에서 삽입된 항-VEGF 전장 항체를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-258 바이러스 게놈 서열. 전이유전자 카세트는 CMV 프로모터, 5' 리더를 가지는 ab 중쇄 서열, P2A 펩타이드 서열, 5' 리더를 가지는 ab 경쇄 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 63 BX 및 BY 영역으로 삽입된 독특한 제한 부위를 가지는 EnAd 게놈을 포함하는 NG-185 바이러스 게놈 서열.
서열 번호 64 박테리아 복제 기원(p15A), 항생제 내성 유전자(KanR) 및 BY 영역에서 삽입된 독특한 제한 부위를 가지는 EnAd 게놈 서열을 포함하는 pNG-33(pColoAd2.4) DNA 플라스미드.
서열 번호 65 박테리아 복제 기원(p15A), 항생제 내성 유전자(KanR) 및 BX 및 BY 영역에서 삽입된 독특한 제한 부위를 가지는 EnAd 게놈 서열을 포함하는 pNG-185(pColoAd2.6) DNA 플라스미드.
서열 번호 66 영역 BY로 삽입된 GAPDH에 대한 shRNA를 암호화하는 전이유전자 카세트를 포함하는 NG-sh01 바이러스 게놈 서열. 전이유전자 카세트는 U6 RNA polIII 프로모터 및 shRNA를 암호화하는 DNA를 함유한다.
서열 번호 67 나트륨 요오다이드 동시수송체(NIS) 아미노산 서열.
서열 번호 68 영역 BY로 삽입된 나트륨 요오다이드 동시수송체(NIS)를 암호화하는 전이유전자 카세트를 포함하는 NG-280 바이러스 게놈 서열. 전이유전자 카세트는 5' SSA, NIS cDNA 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 69 영역 BY로 삽입된 항-VEGF ScFv 및 항-PD-L1 ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-272 바이러스 게놈 서열. 전이유전자 카세트는 SSA, 5' 리더 및 3' 6xHis 태그를 가지는 항-PD-L1 ScFv 서열, P2A 펩타이드 서열, 5' 리더 및 3' V5-태그를 가지는 항-VEGF ScFv 서열 및 3' 폴리(A) 서열을 함유한다.
서열 번호 70 항-CTLA-4 VH 사슬 아미노산 서열.
서열 번호 71 항-CTLA-4 VL 사슬 아미노산 서열.
서열 번호 72 영역 Bx로 삽입된 항-VEGF ScFv를 암호화하는 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-257 바이러스 게놈 서열. 전이유전자 카세트는 bSA, 5' 리더 및 3' 6xHis 태그를 가지는 항-VEGF ScFv 서열, 이어서 3' 폴리(A) 서열을 함유한다.
서열 번호 73 영역 Bx로 삽입된 항-VEGF ScFv를 암호화하는 전이유전자 카세트 및 영역 BY로 삽입된 항-PD-L1 ScFv를 암호화하는 제2 전이유전자 카세트를 가지는 EnAd 게놈을 포함하는 NG-281 바이러스 게놈 서열. 전이유전자 카세트는 bSA, 5' 리더 및 3' 6xHis 태그를 가지는 항-VEGF ScFv 서열, 이어서 3' 폴리(A) 서열을 함유한다.
[도면의 간단한 설명]
도 1은 아데노바이러스 제조 공정의 도식적 표현을 나타낸다.
도 2는 연속 바이러스 제조를 위한 세포 배양 배열을 보여준다.
도 3은 수율에 대한 시드 밀도의 도면을 보여준다.
도 4는 12.5의 감염 다중도 및 1×106의 시드 세포 밀도 및 배지 변경을 가지는 진탕 플라스크 A 및 B에 대한 감염 후 다양한 시점에서의 세포마다 제조된 바이러스의 도면을 보여준다.
도 5는 12.5의 감염 다중도 및 4×106의 시드 세포 밀도 및 배지 변경을 가지는 진탕 플라스크 C 및 D에 대한 감염 후 다양한 시점에서의 세포마다 제조된 바이러스의 도면을 보여준다.
도 6은 배지 변경이 바이러스 수율을 증가시킨다는 것을 보여준다.
도 7은 감염의 기간에 대한 세포의 생존능력 백분율을 보여준다.
도 8은 12.5의 감염 다중도 및 1×106의 시드 밀도와 배지 변경을 가지는 DOE(??) F플라스크에 대한 감염 후 다양한 시점에서의 세포 생존능력의 백분율을 보여준다.
도 9는 12.5의 감염 다중도 및 1×106의 시드 밀도와 배지 변경을 가지는 DOE 플라스크 A 및 B에 대한 감염 후 다양한 시점 동안 상청액에서의 바이러스의 백분율을 보여준다.
도 10은 12.5의 감염 다중도 및 4×106의 시드 밀도와 함께 배지 변경이 없는 DOE 플라스크 C 및 D에 대한 감염 후 다양한 시점 동안 상청액에서의 바이러스의 백분율을 보여준다.
도 11은 12.5ppc의 MOI 및 1×106개의 세포/㎖의 세포 밀도에서 EnAd에 의해 감염된 세포를 가지는 5ℓ의 바이오반응기 공정의 바이러스 생성물 분포를 보여준다.
도 12는 1.9×106개의 세포/㎖의 시드 밀도를 이용한 공정에 대한 시간에 따른 세포마다 제조된 전체 바이러스 입자를 보여주고, 배양물은 50ppc에서 EnAd에 의해 감염된다.
도 13은 12.5ppc 및 1×106vp/㎖의 시드 밀도를 이용한 공정에 대한 시간에 따른 세포 생존능력을 보여준다.
도 14는 50ppc 및 1.9×106vp/㎖의 시드 밀도를 이용한 공정에 대한 시간에 따른 세포 생존능력을 보여준다.
도 15는 2개의 공정(하나는 1×106vp/㎖의 시드 밀도 및 12.5ppc를 이용하고, 제2 공정은 1.9×106vp/㎖ 및 50ppc를 이용함)에 대한 바이오반응기 데이터를 보여준다.
도 16은 2.2×106개의 생존가능 세포/㎖의 시드 밀도 및 50ppc 감염을 이용한 공정에 대한 시간에 따른 세포 생존능력을 보여준다.
도 17은 2.2×106개의 생존가능 세포/㎖의 시드 밀도 및 50ppc 감염을 이용한 공정에 대한 세포 분포를 보여준다.
도 18은 2.2×106개의 생존가능 세포/㎖의 시드 밀도를 이용하고 50ppc에 의해 감염되지만 세포 현탁액 제거가 없거나 새로운 세포 및 배지의 첨가가 없는 대조군 배양에 대한 생존능력을 보여준다.
도 19는 2.2×106개의 생존가능 세포/㎖의 시드 밀도를 이용하고 50ppc에 의해 감염되지만 세포 현탁액 제거가 없거나 새로운 세포 및 배지의 첨가가 없는 대조군 배양에 대한 바이러스 분포를 보여준다.
도 20은 2.2×106개의 생존가능 세포/㎖의 시드 밀도 및 50ppc 감염을 이용한 공정 및 세포 현탁액 제거 없음 및 새로운 세포 및 배지의 첨가 없음이 생략된 대조군 공정에 대한 누적 바이러스 분포를 보여준다.
도 21은 진탕 플라스크 실험에서 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 이용한 공정에 대한 시간에 따른 세포 생존능력을 보여준다.
도 22는 진탕 플라스크 실험에서 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 이용한 공정에 대한 시간에 따른 바이러스 분포를 보여준다.
도 23은 진탕 플라스크 실험에서 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 이용한 대조군 공정(여기서, 생략은 세포 현탁액 제거이고 새로운 세포 및 배지가 첨가되지 않음)에 대한 시간에 따른 세포 생존능력을 보여준다.
도 24는 진탕 플라스크 실험에서 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 이용한 대조군 공정(여기서, 생략은 세포 현탁액 제거이고 새로운 세포 및 배지가 첨가되지 않음)에 대한 시간에 따른 바이러스 분포를 보여준다.
도 25는 진탕 플라스크 실험에서 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 이용한 공정 및 대조군 공정(여기서, 생략은 세포 현탁액 제거이고 새로운 세포 및 배지가 첨가되지 않음)에 대한 누적 전체 바이러스 수율을 보여준다.
도 26은 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 가지는 공정(여기서, 95% 현탁액은 다양한 시점에서 제거되고 1.0x106개의 생존가능 세포/㎖의 새로운 배지 세포 밀도에서의 47.5㎖의 감염되지 않은 세포에 의해 대체됨)에서의 세포의 생존능력을 보여준다.
도 27은 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 가지는 공정(여기서, 95% 현탁액은 다양한 시점에서 제거되고 1.0x106개의 생존가능 세포/㎖의 새로운 배지 세포 밀도에서의 47.5㎖의 감염되지 않은 세포에 의해 대체됨)에서의 바이러스 분포를 보여준다.
도 28은 1.0x106개의 생존가능 세포/㎖ 및 12.5ppc 감염을 가지는 공정(여기서, 95% 현탁액은 다양한 시점에서 제거되고 1.0x106개의 생존가능 세포/㎖의 새로운 배지 세포 밀도에서의 47.5㎖의 감염되지 않은 세포에 의해 대체됨) 및 대조군 공정에서의 누적 전체 바이러스를 보여준다.
실시예
실시예 1
현탁 배양 및 혈청 비함유 배지 채택 HEK293 세포를 성장시키고, 100rpm에서 복수의 125㎖의 진탕 플라스크에서 30㎖의 EX-CELL 혈청 비함유 배지 중에 90% 초과의 생존능력으로 1×106개의 생존가능 세포/㎖의 세포 밀도로 증식시켰다. 이후, 플라스크의 일부를 세포마다 50개의 바이러스 입자(ppc)의 감염 다중도(MOI)를 이용하여 EnAd 바이러스에 의해 감염시키고, 나머지는 감염되지 않게 두고, 계대배양에 의해 0.5 내지 2×106개의 생존가능 세포/㎖의 세포 밀도 및 75% 초과의 생존능력에서 유지시키고, 하기 표시된 바대로 다시 감염된 세포 배양에 대한 첨가를 위해 사용하였다. 감염 후 다양한 시점에서, 10㎖의 배양물을 진탕 플라스크 중 하나로부터 제거하고, 1×106개의 생존가능 세포/㎖ 및 90% 초과의 생존능력에서 새로운 EX-CELL 배지에서 10㎖의 감염되지 않은 HEK293 세포에 의해 대체하였다. 10㎖의 감염된 세포 배양 현탁액을 원심분리하고, 상청액을 분석을 위해 저장하고, 세포 펠렛을 1㎖의 새로운 배지(3X 동결-해동) 중에 용해시키고, 이것을 이후 분석을 위한 저장 전에 원심분리를 위해 청징하였다. 이후, 세포 생존능력이 75% 미만으로 감소할 때까지 감염된 진탕 플라스크 배양물을 재배양하고, 이후 배양을 종료시키고 원심분리하고 처리하여 상청액 및 세포 용해물 분획(상기 기재된 바와 같음)을 생성하고, 이것을 분석을 위해 저장하였다. 실험에 걸쳐, 부르커(Burker) 세포 혈구계산기 및 트립판 블루 염색, 및 pH 측정을 이용하여 세포 및 생존능력 계수를 위해 적은 분취량(50㎕)의 현탁액을 각각의 플라스크로부터 제거하였다.
세포 용해물 및 상청액 샘플에서의 전체 및 감염성 EnAd 입자를 각각 HPLC 및 면역염색 감염 검정에 의해 결정하였다. 상청액 샘플에서의 숙주 세포 DNA의 양을 실시간 qPCR에 의해 결정하고, 상청액 중의 숙주 세포 단백질의 양을 친화도 정제 염소 항-HEK 항혈청을 이용하여 HEK293 숙주 세포 단백질 ELISA 키트를 이용하여 결정하였다. 전체 EnAd 바이러스 수율 및 숙주 세포 DNA 및 단백질의 수준을 상이한 처리 시점에 대해 비교하였다.
실시예 2
실험 프로토콜을 실시예 1에 기재된 바대로 이용하지만, 30㎖의 현탁액 중 15㎖를 다양한 시점에서 제거하고, 10㎖의 용적 대신에 새로운 배지에서 15㎖의 감염되지 않은 세포에 의해 대체하였다.
실시예 3
실험 프로토콜을 실시예 1에 기재된 바대로 이용하지만, 이 실험에서 감염된 배양 플라스크에 다시 첨가된 새로운 감염되지 않은 세포를 이 동일한 플라스크로부터 제거된 바이러스 함유 상청액 중에 재현탁시키고(분석을 위해 2×500㎕의 분취량을 다시 유지시킴), 원래 플라스크로 다시 첨가하기 전에 용적을 10㎖로 조정하였다.
실시예 4
실험 프로토콜을 실시예 3에 기재된 바대로 이용하지만, 30㎖의 현탁액 중 15㎖를 다양한 시점에서 제거하고, 10㎖의 용적 대신에 15㎖의 바이러스 함유 상청액 중에 재현탁된 감염되지 않은 세포에 의해 대체하였다.
실시예 5 내지 8
일련의 4개의 추가의 실험은, 1회차의 현탁액 제거 및 새로운 세포에 의한 대체 후 실험을 종료하기보다는, 실시예 1-4에 기재된 프로토콜을 이용하고, 배양물은 반복적인 현탁액 제거 및 새로운 세포 대체 프로토콜에서 유지되고, 이 프로토콜은 (예를 들어, 세포 부스러기 또는 다른 세포 생성물의 축적 또는 충분한 영양소의 결여로 인해) 충분한 생존가능 세포의 유지가 지속할 수 없을 때까지 계속되었다. 현탁액 제거 및 대체의 빈도는 육안 검사와 함께 매일의 세포 계수 및 생존능력 정보에 의해 지도되었다.
실시예 9
현탁액 HEK293 세포의 단일 바이알을 해동하고, 5ℓ의 교반 탱크(유리 용기) 바이오반응기에서 3ℓ의 워킹 용적으로 증식 전에 진탕 플라스크에서 증식시켰다. 바이오반응기 제어기를 37℃, 7.4의 pH 설정, 50의 용존 산소(DO), 100㎖/분의 송풍량 및 100rpm의 교반의 매개변수로 설정한다. 바이오반응기 시스템을 평형화시킨 후, 1.5ℓ의 EX-CELL 배양 배지의 초기 용적을 5×105개의 세포/㎖의 생존가능 세포 밀도로 시딩하고, 이후 3ℓ의 워킹 용적으로 증식시켜서, 90% 초과의 생존능력을 유지시키고 EnAd 바이러스에 의한 감염을 위해 1.8 내지 2.2×106개의 세포/㎖의 밀도를 목표하였다. 관류 및 배지 교환을 중공 섬유 접선 흐름 여과(TFF)를 통해 3ℓ의 단에서 개시시켰다. TFF 카트리지는 0.2㎛의 기공 크기, 0.5㎜의 루멘 직경 및 790㎠의 표면적을 가졌다. TFF 어셈블리를 오토클레이빙에 의해 예비 살균하고, 이후 무균 배관 용접장치의 사용을 통해 바이오반응기에 부착시켰다. 연동 펌프를 사용하여, 세포 배양물을 TFF 시스템을 통해 재순환시켰다. 보유액 라인에 배압이 위치하지 않았고, 시스템으로부터 측정된 유속을 제공하기 위해 투과액 라인에 제2 연동 펌프가 위치하였다. 관류 유속을 매일 1 용기 용적(즉, 3ℓ/24시간)으로 설정하였다. 목표 세포 밀도가 도달하면, 배양물을 50ppc의 MOI로 EnAd에 의해 감염시켰다.
바이오반응기 배양과 동시에, HEK293 세포의 진탕 플라스크 배양을 규칙적인 계대배양에 의해 0.5 내지 2×106개의 세포/㎖의 세포 밀도 및 90% 초과의 생존능력으로 확립하고 유지시키고, 이 세포를 기재된 바대로 바이오반응기에 첨가하도록 감염되지 않은 세포의 공급원으로서 사용하였다.
관류 투과액의 수집을 감염 후 48시간에 시작하고, 관류 투과액 샘플을 규칙적인 시점에서 취하고, 제2 현탁액 플라스크로부터의 감염되지 않은 세포는 바이오반응기에서 감염된 세포에 다시 첨가되어서, 2×106개의 세포/㎖의 세포 밀도 및 75% 초과의 생존능력의 세포 생존능력을 유지시켰다. 세포 투과액 샘플에서의 전체 및 감염성 EnAd 입자를 각각 HPLC 및 면역염색 감염 검정에 의해 결정하였다. 상청액 샘플에서의 숙주 세포 DNA의 양을 실시간 qPCR에 의해 결정하고, 숙주 세포 단백질의 양을 친화도 정제 염소 항-HEK 항혈청을 이용하여 HEK293 숙주 세포 단백질 ELISA 키트를 이용하여 결정하였다. 다양한 시점에서 전체 EnAd 바이러스 수율 및 숙주 세포 DNA 및 단백질의 수준을 비교하였다. 세포 생존능력이 50% 초과로 유지될 수 없을 때까지, 바이오반응기 배양을 관류 및 새로운 감염되지 않은 세포 보충 절차에 의해 활동적으로 유지시켰다.
수집된 바이러스 함유 투과액 샘플을 혼주하고, 바이러스를 (하기 기재된) EnAd 바이러스의 GMP 제조에 대해 이전에 확립된 공정에 의해 정제하여서, 분석 검정 데이터는 본 명세서에 기재된 연속 제조의 구성요소 없이 제조된 적절한 표준 바이러스 제제와 비교될 수 있었다.
EnAd 바이러스 정제
바이러스를 상이한 시점에서 바이오반응기로부터 수집된 세포 비함유 투과액 샘플로부터 정제하였다. 이 투과액 샘플을 처음에 벤조나제(Benzonase)(등록상표)에 의해 처리하여 숙주 세포 DNA를 분해하고, 이후 2개의 상이한 음이온 교환 크로마토그래피 수지를 이용한 EnAd의 선택적 포획 및 용리를 포함하는 다운스트림에서 2단계 정제 공정의 제1에 대한 제조에서 접선 흐름 여과(TFF)에 의해 농축시키고 완충제 교환시켰다. 제1 일차 포획 단계(예를 들어, 사르토바인드(Sartobind) 수지)에 제2 "연마 정제 단계(예를 들어, CIM-Q)가 후행하여 숙주 세포 잔류물질을 추가로 감소시켰다. 이후, 분석 전에 -80℃에서 재료를 냉동 저장하기 전에, 제2 TFF 단계를 이용하여 정제된 바이러스를 최종 제제 완충제로 완충제 교환하였다.
실시예 10
실시예 9에 기재된 바와 같은 실험 프로토콜을 HEK293 세포의 EnAd 감염에 사용된 100의 MOI로 이용하였다.
실시예 11
이 실험을 위해, 바이러스 수율 및 상청액 또는 세포로의 분포에 대한 상이한 배양 매개변수의 영향을 평가하기 위해 실험의 설계(DOE) 접근법이 JMP 소프트웨어를 이용하여 따라지고 맞춤화되었다. 이 연구의 설계에 사용된 상이한 변수, 각각의 변수 및 반응의 범위는 표 1에 기재되어 있다.
Figure pct00003
워킹 세포 은행(working cell bank; WCB)으로부터의 HEK293 세포의 하나의 바이알을 37℃에서 해동하고 6mM L-글루타메이트가 보충된 EX-CELL 293 배지(성장 배지)를 사용하여 75㎠의 세포 배양 플라스크에서 증식시켰다. 항온처리 4일 후, 세포를 추가로 계대배양(계대배양 1)시키고, 1.0x106개 초과의 생존가능 세포/㎖의 밀도로 1.2×107개의 세포의 생존가능 세포가 달성되면(계대배양 후 대략 3-5일), 세포를 무균 진탕기 플라스크(계대배양 2)로 옮겼다. 배양을 모니터링하고, 세포 수가 1.2×106개 이상의 생존가능 세포/㎖로 배가될 때, 세포를 대략 3일 또는 4일마다 추가로 계대배양하였다. 세포 수를 계수에 의해 세포 확장기에 걸쳐 모니터링하여, 세포 밀도가 0.5-0.6x106개의 생존가능 세포/㎖의 최소 세포 밀도로 유지되게 보장하였다.
세포 계수를 자동 세포 계수기(인비트로겐(Invitrogen)) 및 트립판 블루 염색(인비트로겐)을 이용하여 수행하였다. 실험에 필요한 세포가 생성될 때까지 세포 수를 1ℓ의 무균 진탕기 플라스크에서 추가로 증식시켰다.
세포 현탁액을 300g에서 5분 동안 원심분리하고, 세포 펠렛을 새로운 성장 배지 중에 재현탁시키고, 세포 현탁액을 표 2에 따라 조정된 시딩 세포 밀도로 각각의 플라스크에서 40㎖의 워킹 용적에서 진탕기 플라스크로 옮겼다. 각각의 진탕기 플라스크를 적절히 표지하였다.
Figure pct00004
EnAd 워킹 바이러스 시드 스톡(WVSS)의 일 바이알을 -70℃ 저장으로부터 제거하고 실온에서 해동하였다. 진탕기 플라스크 A 내지 I의 감염을 표 2에 따라 감염 다중도(MOI 또는 ppc)를 이용하여 수행하였다. 음성 대조군 플라스크를 감염시지 않고, 감염의 기간에 걸쳐 유지시켰다. 모든 진탕기 플라스크를 +37℃, 5% CO2 및 120rpm에서 진탕 항온처리기에 위치시켰다. 300g에서 5분 원심분리 후 상청액을 제거하고 각각의 플라스크에서 40㎖의 새로운 성장 배지에서 세포 펠렛을 재현탁시킴으로써 감염 후 24시간에 배지 변경을 진탕 플라스크 A, B, G 및 I에서 수행하였다.
Figure pct00005
감염 후 40시간, 48시간, 60시간 및 72시간에, 4.1㎖의 샘플을 분석을 위해 각각의 플라스크로부터 취하고, 감염 후 96시간에 남은 세포 현탁액 모두를 수확하였다. 각각의 시점에서의 2×500㎕의 샘플을 세포 계수 및 생존능력 분석에 사용하였다. 남은 4.0㎖를 상청액과 세포 펠렛 사이의 바이러스 분포의 분석을 위해 사용하였다. 이것을 분석을 위해 저장된 감염된 세포 배양 현탁액 및 상청액 및 새로운 배지에서 용해된 (3X 냉동-해동) 세포 펠렛(이것은 이후 분석을 위한 저장 전에 원심분리에 의해 청징됨)의 원심분리에 의해 결정하였다.
미정제 바이러스 용해물(Crude Viral Lysate; CVL) 및 상청액(supernatant; SN) 샘플에서의 전체 바이러스 입자 농도(vp)를 AEX-HPLC 검정에 의해 측정하였다. AEX-HPLC 분석 동안, 숙주 세포 단백질(HCP)은 용리 실행의 시작 시 용리하고, 따라서 HCP 함량이 크로마토그램 및 HCP 피크 면적의 크기의 분석에 의해 또한 결정될 수 있다는 것이 공지되어 있다.
세포 생존능력 백분율 및 트립판 블루 염색된 세포 백분율(트립판 블루 염색이 세포에 진입할 수 있도록 아직 기능상 죽지 않았지만, 이의 막 통합성이 손상된 죽은 세포 및 "누출된" 세포 둘 다를 나타냄)가 표 4에 표시되어 있다. 각각의 샘플 시점에 대한 진탕기 플라스크 배양마다 바이러스 입자의 전체 수 및 SN 및 CVL에서의 바이러스 입자의 백분율은 표 5에 표시되어 있다.
상이한 변수 및 바이러스 수율(vp/세포)과 관련하여 반응에 대한 변수의 영향 사이의 관계식을 평가하기 위해 JMP 소프트웨어 분석을 이용하여 '최소 자승 일치 모델'을 이용하여 이 DOE 실험으로부터의 결과를 작도하였다. 3개의 상호작용 관계식은 세포 모델마다 수율에서 상당한 것으로 관찰되었고, 이것은 1) DOI에 대한 시딩 밀도; 2) DOI에 대한 MOI 및 3) DOI에 대한 배지 변경이었다.
시딩 밀도는 전체 바이러스 제조에 가장 큰 통계 효과를 가졌다. 더 낮은 세포 시딩 밀도에서 세포마다 더 높은 수율이 관찰되었다(도 3). 198,143vp/세포의 가장 높은 수율(도 3 및 도 4)은 (12.5ppc에 의해 감염된) 4e6개의 세포/㎖와 비교하여 (12.5ppc에 의해 감염된) 1e6개의 세포/㎖의 가장 낮은 세포 밀도에서 관찰되었고, 세포마다 바이러스 수율은 5922vp/세포로 유의적으로 적었다(도 3 및 도 5). 플라스크마다 전체 바이러스 수율은 더 높은 세포 밀도 및 MOI 조건에서 가장 높았다(플라스크 I).
배지 변경은 또한 수율에 유의적인 통계 효과를 가졌다(도 6). 감염 24시간 후에 배지 변경을 가진 진탕기 플라스크(진탕기 플라스크 A, B, G 및 I)는 배지 변경을 가지지 않은 진탕기 플라스크와 비교하여 더 높은 바이러스 수율을 가졌다. 결과는 도 4, 도 5 및 도 6에 도시되어 있다.
더 높은 감염 기간(DOI)에, 세포 생존능력은 감소하였고, 누출된 세포 및 죽은 세포의 백분율은 증가하였다. 생존능력 및 누출/죽은 (%) 데이터는 표 4에 표시되어 있고, 도 7 및 도 8에 도시되어 있다. 누출된 세포는 온전한 세포막을 가지는 것으로 보이지만 막이 트립판 블루 염색에 투과성인 세포로서 정의된다. 죽은 세포는 트립판 블루 염색되지만, 세포막은 더 이상 온전하지 않았다.
배지 변경을 가지는 12.5ppc에 의해 감염된 1e6개의 세포/㎖ 시딩 밀도에서, 93% 초과의 바이러스가 감염 후 96시간에 상청액에서 관찰되었다(도 9). 배지 변경이 없는 동일한 12.5ppc에 의해 감염된, 4e6개의 세포/㎖의 더 높은 세포 밀도에서, 상청액에서 바이러스가 관찰되지 않았다(도 10).
Figure pct00006
Figure pct00007
Figure pct00008
Figure pct00009
실시예 12
진탕 플라스크에서 실시예 11에 기재된 실험으로부터 나타난 중요한 DOE 매개변수를 5ℓ의 바이오반응기에서 평가하였다. HEK293 세포 증식 및 바이오반응기 준비를 실시예 9에 기재된 바대로 수행하였다. 세포 계수를 자동 세포 계수기 및 트립판 블루 염색을 이용하여 수행하였다. 전체 7.5e8개의 세포의 생존가능 세포가 진탕 플라스크에서 획득되면, 5ℓ의 바이오반응기를 접종하도록 세포를 사용하였다. 1e6개의 세포/㎖의 목표 세포 밀도가 달성될 때, 바이오반응기를 12.5ppc의 MOI에서 EnAd에 의해 감염시켰다.
감염 후 24, 40, 48, 65 및 70시간에, 샘플(20㎖)을 세포 계수, 생존능력 및 전체 바이러스 농도를 위해 취했다. 상청액을 원심분리에 의해 세포로부터 분리시키고, AEX-HPLC에 의한 바이러스 입자 농도 분석을 수행할 때까지 -80℃에서 저장하였다. 세포 펠렛 샘플을 실시예 11에 기재된 바대로 제조하고, 분석까지 -80℃에서 저장하였다. 결과가 표 6에 기재되어 있다.
이 조건 하에, 대부분의 바이러스는 감염 후 71시간까지 상당한 바이러스가 상청액에 존재하지 않으면서 모든 시점에 CVL에 존재하였다(도 11). 감염 후 71시간에, 불과 4%가 상청액에 존재하면서(표 6), 96%의 EnAd 바이러스가 세포 펠렛에서 관찰되었다(표 6 및 도 11). 전체 428,868개의 바이러스 입자를 화학 용해에 의해 세포로부터 추출하고, 세포 부스러기를 청징에 의해 제거하였다(표 6에서 용해 후 수를 참조).
12.5ppc에 의한 감염 후 71시간에 세포 생존능력은 T0에서 85%와 비교하여 58%이었다(도 13). 50ppc에 의한 감염 후 71시간에(실시예 13 참조), 세포 생존능력은 통상적으로 30% 미만이었다.
Figure pct00010
실시예 13
실시예 12에 기재된 바와 같은 실험 프로토콜을 1.9e6개의 세포/㎖의 감염에서 목표 세포 밀도에 의해 이용하고, 배양물을 50ppc의 MOI로 EnAd에 의해 감염시켰다. 샘플을 감염 후 24시간, 48시간, 60시간 및 70시간에 취했다. 샘플의 바이러스 입자 농도를 AEX-HPLC에 의해 분석하고, 결과가 표 7에 기재되어 있다.
감염 후 71시간에, 41%(63,081vp)가 세포 바이러스 용해물에 존재하면서(표 7 및 도 12), 59%(89,485vp)의 EnAd 바이러스가 상청액에서 관찰되었다(표 8). 전체 213,981개의 바이러스 입자를 화학 용해에 의해 세포로부터 추출하고, 세포 부스러기를 청징에 의해 제거하였다(표 7에서 용해 후 수를 참조).
이 실험에서, 50ppc에 의해 감염된 1.9e6개의 세포/㎖의 바이오반응기 배양 매개변수는 실시예 12(여기서, 428,868vp/세포는 12.5ppc에 의해 감염된 1e6개의 세포/㎖로 생성됨)에 기재된 매개변수와 비교하여 수율(213981vp/세포)의 절반을 생성시켰다.
50ppc에 의한 감염 후 71시간에 세포 생존능력은 T0에서 96%와 비교하여 26%이었다(도 14). (실시예 12에서) 12.5ppc에 의한 감염 후 71시간에 세포 생존능력은 T0에서 85%와 비교하여 58%이었다(도 13). 감염에서 낮은 MOI(12.5ppc)는 71시간에 더 높은 (감염 후) 세포 생존능력(58%)을 차지할 수 있고, 이것은 이 연구와 비교하여 실시예 12에서 용해 후 2배 더 높은 바이러스 제조에 잠재적으로 기여한다(도 15).
Figure pct00011
실시예 14
실험 프로토콜을, 25㎖의 진탕기 플라스크 워킹 용적, 2.2×106개의 생존가능 세포/㎖의 세포 밀도 및 50ppc 감염을 제외하고는, 실시예 1에 기재된 바대로 이용하였다. 연속 제조 접근법의 원칙을 조사하기 위해, 다양한 시점에서, 25㎖의 현탁액(80%) 중 20㎖를 제거하고, 새로운 배지에서 시작(2.2×106개의 생존가능 세포/㎖)과 동일한 세포 밀도로 20㎖의 감염되지 않은 세포에 의해 대체하였다. 실험을 7일 동안 계속하고, 매일(1일, 2일, 3일, 4일, 5일, 6일 및 7일)에 20㎖의 감염 후 샘플을 세포 계수, 생존능력 및 상청액 및 CVL에서의 전체 바이러스 농도를 위해 취했다. 감염 후 세포 계수를 혈구계산기 및 트립판 블루 염색(인비트로겐)을 이용하여 수행하였다. 상청액을 원심분리에 의해 세포로부터 제거하고, AEX-HPLC에 의한 바이러스 입자 농도 분석을 위해 -80℃에서 저장하였다. 세포 펠렛 샘플을 실시예 11에 기재된 바대로 제조하고, HPLC 분석까지 -80℃에서 저장하였다. 생존능력 결과는 표 8에 기재되어 있고, 상청액 및 CVL에 대한 HPLC 결과는 표 9에 기재되어 있다.
이 실험에 대한 실험 대조군으로서 대조군 진탕기 플라스크를 25㎖의 워킹 용적, 2.2×106개의 생존가능 세포/㎖의 세포 밀도 및 50ppc에 의한 감염에 의해 설정하였다. 감염 후 세포 현탁액 제거 또는 새로운 세포 및 배지의 첨가가 수행되지 않았다. 이 대조군을 감염 후 3일에 종료시켰다. 세포 계수 및 생존능력을 매일 평가하고, 감염 후 3일에 샘플을 세포 계수, 생존능력 및 전체 바이러스 농도를 위해 취했다. 샘플을 HPLC에 의한 상청액 및 CVL 분석을 위해 실시예 11에 기재된 바대로 처리하였다. 결과는 표 9에 기재되어 있다.
1일에 세포 생존능력은 94%이었고, 이것은 7일에 5%로 가장 낮은 생존능력에 의해 시간에 따라 감소하였다. 누출된 세포의 백분율은 7일에 존재하는 가장 높은 양에 의해 시간에 따라 증가하였다(86%)(표 8 및 도 16 참조). 1일에 대조군 세포 생존능력은 또한 94%이었고, 이것은 3일에 가장 낮은 생존능력(34%)에 의해 감소하였다. 누출된 세포의 백분율은 시간에 따라 증가하고 3일에 최고였다(64%)(표 8 및 도 18).
Figure pct00012
상청액에서의 바이러스의 백분율은 5일 및 6일(각각 46% 및 47%)에 최대로 3일로부터 7일로 변했다. 7일에 불과 17%가 상청액에 존재하였다(표 9 및 도 17). 대조군은 3일에 상청액에 존재하는 74%의 바이러스를 가졌다. 대조군 CVL의 경우, 3일에 불과 26%의 바이러스가 세포에 있었고, 여기서 연속 제조 시험 플라스크에서처럼 53% 이상이 1일 내지 7일에 세포에 있고, 83%는 7일에 세포에 존재하였다(표 9, 도 19).
이 연구를 위해, 7일에 걸쳐 감염된 세포 배양물에 대한 새로운 감염되지 않은 세포의 매일의 첨가에 의해 생성된 전체 누적 바이러스 입자는 1.36e13vp였고, 이것은 대조군 플라스크에서 생성된 양(2.0e12vp)보다 7배 높았다. 7일에 걸쳐 상청액에서의 전체 양은 3.7e12vp였고, 이것은 전체 vp(1.36e13vp)의 27%를 나타내고, 7일 동안 CVL에 73%가 존재한다. 비교하면, 대조군의 바이러스 분포는 상청액에 존재하는 74%(1.5e12vp)와 CVL에 존재하는 26%(5.2e11vp)이었다(표 9 및 표 10, 도 20).
Figure pct00013
Figure pct00014
실시예 15
실험 프로토콜을, 50㎖의 진탕기 플라스크 워킹 용적, 1.0x106개의 생존가능 세포/㎖의 세포 밀도 및 12.5ppc 감염을 제외하고는, 실시예 14에 기재된 바대로 이용하였다. 다양한 시점에서, 50㎖의 현탁액(80%) 중 40㎖를 제거하고, 새로운 배지에서 1.0x106개의 생존가능 세포/㎖로 40㎖의 감염되지 않은 세포에 의해 대체하였다. 실험을 5일 동안 계속하고, 감염 후 매일(1일, 2일, 3일, 4일 및 5일)에 샘플을 세포 계수, 생존능력 및 전체 바이러스 농도를 위해 취했다. 감염 후 세포 계수를 혈구계산기 및 트립판 블루 염색(인비트로겐)을 이용하여 수행하였다. 상청액을 원심분리에 의해 세포로부터 제거하고, AEX-HPLC에 의한 바이러스 입자 농도 분석을 수행할 때까지 -80℃에서 저장하였다. 세포 펠렛 샘플을 실시예 11에 기재된 바대로 제조하고, HPLC 분석까지 -80℃에서 저장하였다. 생존능력 결과는 표 11에 기재되어 있다. 상청액 및 CVL에 대한 HPLC 결과는 표 12에 기재되어 있다.
1일에 세포 생존능력은 78%이었고, 이것은 7일에 가장 낮은 생존능력(16%)에 의해 시간에 따라 감소하였다. 누출된 세포의 백분율은 3일에 존재하는 가장 높은 양에 의해 시간에 따라 증가하였다(82%)(표 11 및 도 21). 1일에 대조군 세포 생존능력은 73%이었고, 이것은 3일에 기록된 가장 낮은 생존능력(23%)에 의해 감소하였다. 누출된 세포의 백분율은 시간에 따라 증가하고 3일에 최고였다(70%)(도 23).
Figure pct00015
상청액에서의 바이러스의 백분율은 4일(각각 28%)에 최대로 1일로부터 5일로 변했다. 5일에 불과 18%가 상청액에 존재하였다(표 12 및 도 22). 대조군(세포 및 배지의 제거 및 대체가 없는 플라스크)은 3일에 상청액에 존재하는 81%의 바이러스 및 CVL에서의 19%를 가졌다.
이 대조군 CVL의 경우, 3일에 불과 19%의 바이러스가 세포에 있었고, 여기서 매일의 세포 및 배지 대체를 가지는 플라스크에서처럼 72% 이상이 1일 내지 5일에 세포 연관된 채 있고, 82%는 5일에 세포 펠렛에 존재하였다(표 12).
이 연구를 위해, 5일에 걸쳐 감염된 세포에 대한 새로운 감염되지 않은 세포의 첨가에 의해 생성된 전체 바이러스 입자는 2.33e13vp였고, 이것은 대조군에서 생성된 양보다 3배 높았다(7.6e12). 5일에 걸쳐 상청액에서의 전체 양은 4.4e12vp였고, 이것은 전체 vp(2.33e13)의 19%를 나타내고, 5일 동안 81%가 CVL에 존재하였다. 비교하면, 대조군 배양의 바이러스 분포는 상청액에 존재하는 81%(6.10e12)와 CVL에 존재하는 19%(1.48e12)이었다(표 12 및 표 13, 도 25).
Figure pct00016
Figure pct00017
실시예 16
실험 프로토콜을 실시예 15에 기재된 바대로 이용하지만, 다양한 시점에서 50㎖의 현탁액(95%) 중 47.5㎖를 제거하고, 1.0x106개의 생존가능 세포/㎖의 동일한 세포 밀도로 새로운 배지에서 47.5㎖의 감염되지 않은 세포에 의해 대체하였다. 이 연구는 실시예 15의 것과 동시에 수행되고, 동일한 대조군 플라스크를 사용하였다. 생존능력 결과는 표 14에 기재되어 있다. 상청액 및 CVL에 대한 HPLC 결과는 표 15에 기재되어 있다.
1일에 세포 생존능력은 78%이었고, 이것은 5일에 가장 낮은 생존능력(16%)에 의해 시간에 따라 감소하였다. 누출된 세포의 백분율은 5일에 존재하는 가장 높은 양에 의해 시간에 따라 증가하였다(77%)(표 14 및 도 26). 1일에 대조군 세포 생존능력은 73%이었고, 이것은 3일에 기록된 가장 낮은 생존능력(23%)에 의해 감소하였다. 누출된 세포의 백분율은 시간에 따라 증가하고 3일에 최고였다(70%)(도 23).
Figure pct00018
1일 내지 5일에, 바이러스가 상청액에 존재하지 않고, 모든 바이러스는 CVL에 있었다(표 15 및 도 27).
이 실험을 위해, 5일에 걸쳐 현탁액의 매일의 제거 및 감염된 세포에 대한 새로운 감염되지 않은 세포의 첨가에 의해 생성된 전체 바이러스 입자는 1.96e13vp였고, 이것은 대조군에서 생성된 양보다 3배 높았다(7.6e12). 5일에 걸쳐 CVL에서의 바이러스의 전체 양은 1.96e13vp였고, 이것은 전체 vp(1.96e13)의 100%를 나타낸다. 비교하면, 대조군의 바이러스 분포는 상청액에 존재하는 81%(6.10e12)와 CVL에 존재하는 19%(1.48e12)였다(표 16 및 도 28).
Figure pct00019
Figure pct00020
SEQUENCE LISTING <110> PSIOXUS THERAPEUTICS LIMITED <120> A Process for the Production of Adenovirus <130> WO 2016/030489 <140> PCT/EP2015/069706 <141> 2015-08-27 <150> GB 1415154.2 <151> 2014-08-27 <150> GB 1415581.6 <151> 2014-09-03 <160> 73 <170> PatentIn version 3.5 <210> 1 <211> 35373 <212> DNA <213> Artificial Sequence <220> <223> NG-77 virus genome sequence <400> 1 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgctgctaa tcctttctct cttcaggcca ccatgggatg 29400 gagctgtatc atcctcttct tggtagcaac agctacaggt gtccactccg aggttcagct 29460 ggtggagtct ggcggtggcc tggtgcagcc agggggctca ctccgtttgt cctgtgcagc 29520 ttctggctat accttcacca actatggtat gaactgggtc cgtcaggccc cgggtaaggg 29580 cctggaatgg gttggatgga ttaacaccta taccggtgaa ccgacctatg ctgcggattt 29640 caaacgtcgt tttacttttt ctctggacac ctccaagagc acagcttacc tgcagatgaa 29700 cagcctgcgc gctgaggaca ctgccgtcta ttactgtgca aagtacccgc actattatgg 29760 gagcagccac tggtatttcg acgtctgggg tcaaggaacc ctggtcaccg tctcctcggc 29820 ctccaccaag ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg 29880 cacagcggcc ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg 29940 gaactcaggc gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg 30000 actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta 30060 catctgcaac gtgaatcaca agcccagcaa caccaaggtc gacaagaaag ttgagcccaa 30120 atcttgtgac aaaactcaca catgcccacc gtgcccagca cctgagctcc tggggggacc 30180 gtcagtcttc ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga 30240 ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta 30300 cgtggacggc gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag 30360 cacgtaccgt gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga 30420 gtacaagtgc aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa 30480 agccaaaggg cagccccgag aaccacaggt gtacaccctg cccccatccc gggaggagat 30540 gaccaagaac caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc 30600 cgtggagtgg gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct 30660 ggactccgac ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca 30720 gcaggggaac gtcttctcat gctccgtgat gcacgaggct ctgcacaacc actacacgca 30780 gaagagcctc tccctgtctc cgggtaaatg atctagactc gaggtttcta gaagttgtct 30840 cctcctgcac tgactgactg acctaacgtt actggccgaa gccgcttgga ataaggccgg 30900 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc 30960 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa 31020 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga 31080 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc 31140 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc 31200 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcccctcaag cgtattcaac 31260 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg 31320 tgcacatgct tttcatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg 31380 gggacgtggt tttcctttga aaaacacgat gataataaca tgggatggag ctgtatcatc 31440 ctcttcttgg tagcaacagc tacaggtgtc cactccgata tccagatgac ccagtccccg 31500 agctccctgt ccgcctctgt gggcgatagg gtcaccatca cctgcagcgc aagtcaggat 31560 attagcaact atttaaactg gtatcaacag aaaccaggaa aagctccgaa agtcctgatt 31620 tacttcacct cctctctcca ctctggagtc ccttctcgct tctctggatc cggttctggg 31680 acggatttca ctctgaccat cagcagtctg cagccagaag acttcgcaac ttattactgt 31740 caacagtata gcaccgtgcc gtggacgttt ggacagggta ccaaggtgga gatcaaacga 31800 actgtggctg caccatctgt cttcatcttc ccgccatctg atgagcagtt gaaatctgga 31860 actgcttctg ttgtgtgcct gctgaataac ttctatccca gagaggccaa agtacagtgg 31920 aaggtggata acgccctcca atcgggtaac tcccaggaga gtgtcacaga gcaggacagc 31980 aaggacagca cctacagcct cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa 32040 cacaaagtct acgcctgcga agtcacccat cagggcctga gctcgcccgt cacaaagagc 32100 ttcaacaggg gagagtgtta agctagcttg actgactgag atacagcgta ccttcagctc 32160 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa 32220 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc 32280 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg 32340 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtagtcgtca gctatcctgc 32400 aggaacttgt ttatttgaaa atcaattcac aaaatccgag tagttatttt gcctccccct 32460 tcccatttaa cagaatacac caatctctcc ccacgcacag ctttaaacat ttggatacca 32520 ttagatatag acatggtttt agattccaca ttccaaacag tttcagagcg agccaatctg 32580 gggtcagtga tagataaaaa tccatcggga tagtctttta aagcgctttc acagtccaac 32640 tgctgcggat gcgactccgg agtctggatc acggtcatct ggaagaagaa cgatgggaat 32700 cataatccga aaacggtatc ggacgattgt gtctcatcaa acccacaagc agccgctgtc 32760 tgcgtcgctc cgtgcgactg ctgtttatgg gatcagggtc cacagtgtcc tgaagcatga 32820 ttttaatagc ccttaacatc aactttctgg tgcgatgcgc gcagcaacgc attctgattt 32880 cactcaaatc tttgcagtag gtacaacaca ttattacaat attgtttaat aaaccataat 32940 taaaagcgct ccagccaaaa ctcatatctg atataatcgc ccctgcatga ccatcatacc 33000 aaagtttaat ataaattaaa tgacgttccc tcaaaaacac actacccaca tacatgatct 33060 cttttggcat gtgcatatta acaatctgtc tgtaccatgg acaacgttgg ttaatcatgc 33120 aacccaatat aaccttccgg aaccacactg ccaacaccgc tcccccagcc atgcattgaa 33180 gtgaaccctg ctgattacaa tgacaatgaa gaacccaatt ctctcgaccg tgaatcactt 33240 gagaatgaaa aatatctata gtggcacaac atagacataa atgcatgcat cttctcataa 33300 tttttaactc ctcaggattt agaaacatat cccagggaat aggaagctct tgcagaacag 33360 taaagctggc agaacaagga agaccacgaa cacaacttac actatgcata gtcatagtat 33420 cacaatctgg caacagcggg tggtcttcag tcatagaagc tcgggtttca ttttcctcac 33480 aacgtggtaa ctgggctctg gtgtaagggt gatgtctggc gcatgatgtc gagcgtgcgc 33540 gcaaccttgt cataatggag ttgcttcctg acattctcgt attttgtata gcaaaacgcg 33600 gccctggcag aacacactct tcttcgcctt ctatcctgcc gcttagcgtg ttccgtgtga 33660 tagttcaagt acaaccacac tcttaagttg gtcaaaagaa tgctggcttc agttgtaatc 33720 aaaactccat cgcatctaat cgttctgagg aaatcatcca agcaatgcaa ctggattgtg 33780 tttcaagcag gagaggagag ggaagagacg gaagaaccat gttaattttt attccaaacg 33840 atctcgcagt acttcaaatt gtagatcgcg cagatggcat ctctcgcccc cactgtgttg 33900 gtgaaaaagc acagctagat caaaagaaat gcgattttca aggtgctcaa cggtggcttc 33960 cagcaaagcc tccacgcgca catccaagaa caaaagaata ccaaaagaag gagcattttc 34020 taactcctca atcatcatat tacattcctg caccattccc agataatttt cagctttcca 34080 gccttgaatt attcgtgtca gttcttgtgg taaatccaat ccacacatta caaacaggtc 34140 ccggagggcg ccctccacca ccattcttaa acacaccctc ataatgacaa aatatcttgc 34200 tcctgtgtca cctgtagcga attgagaatg gcaacatcaa ttgacatgcc cttggctcta 34260 agttcttctt taagttctag ttgtaaaaac tctctcatat tatcaccaaa ctgcttagcc 34320 agaagccccc cgggaacaag agcaggggac gctacagtgc agtacaagcg cagacctccc 34380 caattggctc cagcaaaaac aagattggaa taagcatatt gggaaccgcc agtaatatca 34440 tcgaagttgc tggaaatata atcaggcaga gtttcttgta aaaattgaat aaaagaaaaa 34500 tttgccaaaa aaacattcaa aacctctggg atgcaaatgc aataggttac cgcgctgcgc 34560 tccaacattg ttagttttga attagtctgc aaaaataaaa aaaaaaacaa gcgtcatatc 34620 atagtagcct gacgaacaga tggataaatc agtctttcca tcacaagaca agccacaggg 34680 tctccagctc gaccctcgta aaacctgtca tcatgattaa acaacagcac cgaaagttcc 34740 tcgcggtgac cagcatgaat aattcttgat gaagcataca atccagacat gttagcatca 34800 gttaacgaga aaaaacagcc aacatagcct ttgggtataa ttatgcttaa tcgtaagtat 34860 agcaaagcca cccctcgcgg atacaaagta aaaggcacag gagaataaaa aatataatta 34920 tttctctgct gctgttcagg caacgtcgcc cccggtccct ctaaatacac atacaaagcc 34980 tcatcagcca tggcttacca gacaaagtac agcgggcaca caaagcacaa gctctaaagt 35040 gactctccaa cctctccaca atatatatat acacaagccc taaactgacg taatgggagt 35100 aaagtgtaaa aaatcccgcc aaacccaaca cacaccccga aactgcgtca ccagggaaaa 35160 gtacagtttc acttccgcaa tcccaacagg cgtaacttcc tctttctcac ggtacgtgat 35220 atcccactaa cttgcaacgt cattttccca cggtcgcacc gcccctttta gccgttaacc 35280 ccacagccaa tcaccacacg atccacactt tttaaaatca cctcatttac atattggcac 35340 cattccatct ataaggtata ttatatagat aga 35373 <210> 2 <211> 35355 <212> DNA <213> Artificial Sequence <220> <223> NG-135 virus genome sequence <400> 2 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgta tcatcctctt 29400 cttggtagca acagctacag gtgtccactc cgaggttcag ctggtggagt ctggcggtgg 29460 cctggtgcag ccagggggct cactccgttt gtcctgtgca gcttctggct ataccttcac 29520 caactatggt atgaactggg tccgtcaggc cccgggtaag ggcctggaat gggttggatg 29580 gattaacacc tataccggtg aaccgaccta tgctgcggat ttcaaacgtc gttttacttt 29640 ttctctggac acctccaaga gcacagctta cctgcagatg aacagcctgc gcgctgagga 29700 cactgccgtc tattactgtg caaagtaccc gcactattat gggagcagcc actggtattt 29760 cgacgtctgg ggtcaaggaa ccctggtcac cgtctcctcg gcctccacca agggcccatc 29820 ggtcttcccc ctggcaccct cctccaagag cacctctggg ggcacagcgg ccctgggctg 29880 cctggtcaag gactacttcc ccgaaccggt gacggtgtcg tggaactcag gcgccctgac 29940 cagcggcgtg cacaccttcc cggctgtcct acagtcctca ggactctact ccctcagcag 30000 cgtggtgacc gtgccctcca gcagcttggg cacccagacc tacatctgca acgtgaatca 30060 caagcccagc aacaccaagg tcgacaagaa agttgagccc aaatcttgtg acaaaactca 30120 cacatgccca ccgtgcccag cacctgagct cctgggggga ccgtcagtct tcctcttccc 30180 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt 30240 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt 30300 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag 30360 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc 30420 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg 30480 agaaccacag gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag 30540 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa 30600 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt 30660 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc 30720 atgctccgtg atgcacgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc 30780 tccgggtaaa tgatctagac tcgaggtttc tagaagttgt ctcctcctgc actgactgac 30840 tgacctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg 30900 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc 30960 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg 31020 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg 31080 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca 31140 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata 31200 gttgtggaaa gagtcaaatg gctcccctca agcgtattca acaaggggct gaaggatgcc 31260 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg cttttcatgt 31320 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt 31380 gaaaaacacg atgataataa catggactgg acctggcgcg tgttttgcct gctcgccgtg 31440 gctcctgggg cccacagcga tatccagatg acccagtccc cgagctccct gtccgcctct 31500 gtgggcgata gggtcaccat cacctgcagc gcaagtcagg atattagcaa ctatttaaac 31560 tggtatcaac agaaaccagg aaaagctccg aaagtcctga tttacttcac ctcctctctc 31620 cactctggag tcccttctcg cttctctgga tccggttctg ggacggattt cactctgacc 31680 atcagcagtc tgcagccaga agacttcgca acttattact gtcaacagta tagcaccgtg 31740 ccgtggacgt ttggacaggg taccaaggtg gagatcaaac gaactgtggc tgcaccatct 31800 gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcttc tgttgtgtgc 31860 ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga taacgccctc 31920 caatcgggta actcccagga gagtgtcaca gagcaggaca gcaaggacag cacctacagc 31980 ctcagcagca ccctgacgct gagcaaagca gactacgaga aacacaaagt ctacgcctgc 32040 gaagtcaccc atcagggcct gagctcgccc gtcacaaaga gcttcaacag gggagagtgt 32100 taagctagct tgactgactg agatacagcg taccttcagc tcacagacat gataagatac 32160 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 32220 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 32280 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc 32340 aagtaaaacc tctacaaatg tggtagtcgt cagctatcct gcaggaactt gtttatttga 32400 aaatcaattc acaaaatccg agtagttatt ttgcctcccc cttcccattt aacagaatac 32460 accaatctct ccccacgcac agctttaaac atttggatac cattagatat agacatggtt 32520 ttagattcca cattccaaac agtttcagag cgagccaatc tggggtcagt gatagataaa 32580 aatccatcgg gatagtcttt taaagcgctt tcacagtcca actgctgcgg atgcgactcc 32640 ggagtctgga tcacggtcat ctggaagaag aacgatggga atcataatcc gaaaacggta 32700 tcggacgatt gtgtctcatc aaacccacaa gcagccgctg tctgcgtcgc tccgtgcgac 32760 tgctgtttat gggatcaggg tccacagtgt cctgaagcat gattttaata gcccttaaca 32820 tcaactttct ggtgcgatgc gcgcagcaac gcattctgat ttcactcaaa tctttgcagt 32880 aggtacaaca cattattaca atattgttta ataaaccata attaaaagcg ctccagccaa 32940 aactcatatc tgatataatc gcccctgcat gaccatcata ccaaagttta atataaatta 33000 aatgacgttc cctcaaaaac acactaccca catacatgat ctcttttggc atgtgcatat 33060 taacaatctg tctgtaccat ggacaacgtt ggttaatcat gcaacccaat ataaccttcc 33120 ggaaccacac tgccaacacc gctcccccag ccatgcattg aagtgaaccc tgctgattac 33180 aatgacaatg aagaacccaa ttctctcgac cgtgaatcac ttgagaatga aaaatatcta 33240 tagtggcaca acatagacat aaatgcatgc atcttctcat aatttttaac tcctcaggat 33300 ttagaaacat atcccaggga ataggaagct cttgcagaac agtaaagctg gcagaacaag 33360 gaagaccacg aacacaactt acactatgca tagtcatagt atcacaatct ggcaacagcg 33420 ggtggtcttc agtcatagaa gctcgggttt cattttcctc acaacgtggt aactgggctc 33480 tggtgtaagg gtgatgtctg gcgcatgatg tcgagcgtgc gcgcaacctt gtcataatgg 33540 agttgcttcc tgacattctc gtattttgta tagcaaaacg cggccctggc agaacacact 33600 cttcttcgcc ttctatcctg ccgcttagcg tgttccgtgt gatagttcaa gtacaaccac 33660 actcttaagt tggtcaaaag aatgctggct tcagttgtaa tcaaaactcc atcgcatcta 33720 atcgttctga ggaaatcatc caagcaatgc aactggattg tgtttcaagc aggagaggag 33780 agggaagaga cggaagaacc atgttaattt ttattccaaa cgatctcgca gtacttcaaa 33840 ttgtagatcg cgcagatggc atctctcgcc cccactgtgt tggtgaaaaa gcacagctag 33900 atcaaaagaa atgcgatttt caaggtgctc aacggtggct tccagcaaag cctccacgcg 33960 cacatccaag aacaaaagaa taccaaaaga aggagcattt tctaactcct caatcatcat 34020 attacattcc tgcaccattc ccagataatt ttcagctttc cagccttgaa ttattcgtgt 34080 cagttcttgt ggtaaatcca atccacacat tacaaacagg tcccggaggg cgccctccac 34140 caccattctt aaacacaccc tcataatgac aaaatatctt gctcctgtgt cacctgtagc 34200 gaattgagaa tggcaacatc aattgacatg cccttggctc taagttcttc tttaagttct 34260 agttgtaaaa actctctcat attatcacca aactgcttag ccagaagccc cccgggaaca 34320 agagcagggg acgctacagt gcagtacaag cgcagacctc cccaattggc tccagcaaaa 34380 acaagattgg aataagcata ttgggaaccg ccagtaatat catcgaagtt gctggaaata 34440 taatcaggca gagtttcttg taaaaattga ataaaagaaa aatttgccaa aaaaacattc 34500 aaaacctctg ggatgcaaat gcaataggtt accgcgctgc gctccaacat tgttagtttt 34560 gaattagtct gcaaaaataa aaaaaaaaac aagcgtcata tcatagtagc ctgacgaaca 34620 gatggataaa tcagtctttc catcacaaga caagccacag ggtctccagc tcgaccctcg 34680 taaaacctgt catcatgatt aaacaacagc accgaaagtt cctcgcggtg accagcatga 34740 ataattcttg atgaagcata caatccagac atgttagcat cagttaacga gaaaaaacag 34800 ccaacatagc ctttgggtat aattatgctt aatcgtaagt atagcaaagc cacccctcgc 34860 ggatacaaag taaaaggcac aggagaataa aaaatataat tatttctctg ctgctgttca 34920 ggcaacgtcg cccccggtcc ctctaaatac acatacaaag cctcatcagc catggcttac 34980 cagacaaagt acagcgggca cacaaagcac aagctctaaa gtgactctcc aacctctcca 35040 caatatatat atacacaagc cctaaactga cgtaatggga gtaaagtgta aaaaatcccg 35100 ccaaacccaa cacacacccc gaaactgcgt caccagggaa aagtacagtt tcacttccgc 35160 aatcccaaca ggcgtaactt cctctttctc acggtacgtg atatcccact aacttgcaac 35220 gtcattttcc cacggtcgca ccgccccttt tagccgttaa ccccacagcc aatcaccaca 35280 cgatccacac tttttaaaat cacctcattt acatattggc accattccat ctataaggta 35340 tattatatag ataga 35355 <210> 3 <211> 34493 <212> DNA <213> Artificial Sequence <220> <223> A virus genome sequence consisting of a transgene cassette that encodes an anti-VEGF full length antibody inserted in the region BY. The transgene cassette contains a SSA, ab heavy chain sequence with 5' leader, a SSA,and ab light chain sequence with <400> 3 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc accatgggat ggagctgtat catcctcttc 29400 ttggtagcaa cagctacagg tgtccactcc gaggttcagc tggtggagtc tggcggtggc 29460 ctggtgcagc cagggggctc actccgtttg tcctgtgcag cttctggcta taccttcacc 29520 aactatggta tgaactgggt ccgtcaggcc ccgggtaagg gcctggaatg ggttggatgg 29580 attaacacct ataccggtga accgacctat gctgcggatt tcaaacgtcg ttttactttt 29640 tctctggaca cctccaagag cacagcttac ctgcagatga acagcctgcg cgctgaggac 29700 actgccgtct attactgtgc aaagtacccg cactattatg ggagcagcca ctggtatttc 29760 gacgtctggg gtcaaggaac cctggtcacc gtctcctcgg cctccaccaa gggcccatcg 29820 gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc 29880 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 29940 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc 30000 gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac 30060 aagcccagca acaccaaggt cgacaagaaa gttgagccca aatcttgtga caaaactcac 30120 acatgcccac cgtgcccagc acctgagctc ctggggggac cgtcagtctt cctcttcccc 30180 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 30240 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 30300 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 30360 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 30420 aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 30480 gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc 30540 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 30600 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 30660 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 30720 tgctccgtga tgcacgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 30780 ccgggtaaat gatctagact cgagcaggcg agcatggact ggacctggcg cgtgttttgc 30840 ctgctcgccg tggctcctgg ggcccacagc gatatccaga tgacccagtc cccgagctcc 30900 ctgtccgcct ctgtgggcga tagggtcacc atcacctgca gcgcaagtca ggatattagc 30960 aactatttaa actggtatca acagaaacca ggaaaagctc cgaaagtcct gatttacttc 31020 acctcctctc tccactctgg agtcccttct cgcttctctg gatccggttc tgggacggat 31080 ttcactctga ccatcagcag tctgcagcca gaagacttcg caacttatta ctgtcaacag 31140 tatagcaccg tgccgtggac gtttggacag ggtaccaagg tggagatcaa acgaactgtg 31200 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgct 31260 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg 31320 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac 31380 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa 31440 gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac 31500 aggggagagt gttaacctgc aggaacttgt ttatttgaaa atcaattcac aaaatccgag 31560 tagttatttt gcctccccct tcccatttaa cagaatacac caatctctcc ccacgcacag 31620 ctttaaacat ttggatacca ttagatatag acatggtttt agattccaca ttccaaacag 31680 tttcagagcg agccaatctg gggtcagtga tagataaaaa tccatcggga tagtctttta 31740 aagcgctttc acagtccaac tgctgcggat gcgactccgg agtctggatc acggtcatct 31800 ggaagaagaa cgatgggaat cataatccga aaacggtatc ggacgattgt gtctcatcaa 31860 acccacaagc agccgctgtc tgcgtcgctc cgtgcgactg ctgtttatgg gatcagggtc 31920 cacagtgtcc tgaagcatga ttttaatagc ccttaacatc aactttctgg tgcgatgcgc 31980 gcagcaacgc attctgattt cactcaaatc tttgcagtag gtacaacaca ttattacaat 32040 attgtttaat aaaccataat taaaagcgct ccagccaaaa ctcatatctg atataatcgc 32100 ccctgcatga ccatcatacc aaagtttaat ataaattaaa tgacgttccc tcaaaaacac 32160 actacccaca tacatgatct cttttggcat gtgcatatta acaatctgtc tgtaccatgg 32220 acaacgttgg ttaatcatgc aacccaatat aaccttccgg aaccacactg ccaacaccgc 32280 tcccccagcc atgcattgaa gtgaaccctg ctgattacaa tgacaatgaa gaacccaatt 32340 ctctcgaccg tgaatcactt gagaatgaaa aatatctata gtggcacaac atagacataa 32400 atgcatgcat cttctcataa tttttaactc ctcaggattt agaaacatat cccagggaat 32460 aggaagctct tgcagaacag taaagctggc agaacaagga agaccacgaa cacaacttac 32520 actatgcata gtcatagtat cacaatctgg caacagcggg tggtcttcag tcatagaagc 32580 tcgggtttca ttttcctcac aacgtggtaa ctgggctctg gtgtaagggt gatgtctggc 32640 gcatgatgtc gagcgtgcgc gcaaccttgt cataatggag ttgcttcctg acattctcgt 32700 attttgtata gcaaaacgcg gccctggcag aacacactct tcttcgcctt ctatcctgcc 32760 gcttagcgtg ttccgtgtga tagttcaagt acaaccacac tcttaagttg gtcaaaagaa 32820 tgctggcttc agttgtaatc aaaactccat cgcatctaat cgttctgagg aaatcatcca 32880 agcaatgcaa ctggattgtg tttcaagcag gagaggagag ggaagagacg gaagaaccat 32940 gttaattttt attccaaacg atctcgcagt acttcaaatt gtagatcgcg cagatggcat 33000 ctctcgcccc cactgtgttg gtgaaaaagc acagctagat caaaagaaat gcgattttca 33060 aggtgctcaa cggtggcttc cagcaaagcc tccacgcgca catccaagaa caaaagaata 33120 ccaaaagaag gagcattttc taactcctca atcatcatat tacattcctg caccattccc 33180 agataatttt cagctttcca gccttgaatt attcgtgtca gttcttgtgg taaatccaat 33240 ccacacatta caaacaggtc ccggagggcg ccctccacca ccattcttaa acacaccctc 33300 ataatgacaa aatatcttgc tcctgtgtca cctgtagcga attgagaatg gcaacatcaa 33360 ttgacatgcc cttggctcta agttcttctt taagttctag ttgtaaaaac tctctcatat 33420 tatcaccaaa ctgcttagcc agaagccccc cgggaacaag agcaggggac gctacagtgc 33480 agtacaagcg cagacctccc caattggctc cagcaaaaac aagattggaa taagcatatt 33540 gggaaccgcc agtaatatca tcgaagttgc tggaaatata atcaggcaga gtttcttgta 33600 aaaattgaat aaaagaaaaa tttgccaaaa aaacattcaa aacctctggg atgcaaatgc 33660 aataggttac cgcgctgcgc tccaacattg ttagttttga attagtctgc aaaaataaaa 33720 aaaaaaacaa gcgtcatatc atagtagcct gacgaacaga tggataaatc agtctttcca 33780 tcacaagaca agccacaggg tctccagctc gaccctcgta aaacctgtca tcatgattaa 33840 acaacagcac cgaaagttcc tcgcggtgac cagcatgaat aattcttgat gaagcataca 33900 atccagacat gttagcatca gttaacgaga aaaaacagcc aacatagcct ttgggtataa 33960 ttatgcttaa tcgtaagtat agcaaagcca cccctcgcgg atacaaagta aaaggcacag 34020 gagaataaaa aatataatta tttctctgct gctgttcagg caacgtcgcc cccggtccct 34080 ctaaatacac atacaaagcc tcatcagcca tggcttacca gacaaagtac agcgggcaca 34140 caaagcacaa gctctaaagt gactctccaa cctctccaca atatatatat acacaagccc 34200 taaactgacg taatgggagt aaagtgtaaa aaatcccgcc aaacccaaca cacaccccga 34260 aactgcgtca ccagggaaaa gtacagtttc acttccgcaa tcccaacagg cgtaacttcc 34320 tctttctcac ggtacgtgat atcccactaa cttgcaacgt cattttccca cggtcgcacc 34380 gcccctttta gccgttaacc ccacagccaa tcaccacacg atccacactt tttaaaatca 34440 cctcatttac atattggcac cattccatct ataaggtata ttatatagat aga 34493 <210> 4 <211> 34754 <212> DNA <213> Artificial Sequence <220> <223> A virus genome sequence consisting of a transgene cassette that encodes an anti-VEGF full length antibody inserted in the region BY. <400> 4 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc accatgggat ggagctgtat catcctcttc 29400 ttggtagcaa cagctacagg tgtccactcc gaggttcagc tggtggagtc tggcggtggc 29460 ctggtgcagc cagggggctc actccgtttg tcctgtgcag cttctggcta taccttcacc 29520 aactatggta tgaactgggt ccgtcaggcc ccgggtaagg gcctggaatg ggttggatgg 29580 attaacacct ataccggtga accgacctat gctgcggatt tcaaacgtcg ttttactttt 29640 tctctggaca cctccaagag cacagcttac ctgcagatga acagcctgcg cgctgaggac 29700 actgccgtct attactgtgc aaagtacccg cactattatg ggagcagcca ctggtatttc 29760 gacgtctggg gtcaaggaac cctggtcacc gtctcctcgg cctccaccaa gggcccatcg 29820 gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc 29880 ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc 29940 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc 30000 gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac 30060 aagcccagca acaccaaggt cgacaagaaa gttgagccca aatcttgtga caaaactcac 30120 acatgcccac cgtgcccagc acctgagctc ctggggggac cgtcagtctt cctcttcccc 30180 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 30240 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 30300 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 30360 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 30420 aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 30480 gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc 30540 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 30600 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 30660 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 30720 tgctccgtga tgcacgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 30780 ccgggtaaat gatctagact cgagcaggcc gacatggact ggacctggcg cgtgttttgc 30840 ctgctcgccg tggctcctgg ggcccacagc gatatccaga tgacccagtc cccgagctcc 30900 ctgtccgcct ctgtgggcga tagggtcacc atcacctgca gcgcaagtca ggatattagc 30960 aactatttaa actggtatca acagaaacca ggaaaagctc cgaaagtcct gatttacttc 31020 acctcctctc tccactctgg agtcccttct cgcttctctg gatccggttc tgggacggat 31080 ttcactctga ccatcagcag tctgcagcca gaagacttcg caacttatta ctgtcaacag 31140 tatagcaccg tgccgtggac gtttggacag ggtaccaagg tggagatcaa acgaactgtg 31200 gctgcaccat ctgtcttcat cttcccgcca tctgatgagc agttgaaatc tggaactgct 31260 tctgttgtgt gcctgctgaa taacttctat cccagagagg ccaaagtaca gtggaaggtg 31320 gataacgccc tccaatcggg taactcccag gagagtgtca cagagcagga cagcaaggac 31380 agcacctaca gcctcagcag caccctgacg ctgagcaaag cagactacga gaaacacaaa 31440 gtctacgcct gcgaagtcac ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac 31500 aggggagagt gttaagctag cttgactgac tgagatacag cgtaccttca gctcacagac 31560 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 31620 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 31680 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 31740 gttttttaaa gcaagtaaaa cctctacaaa tgtggtcctg caggaacttg tttatttgaa 31800 aatcaattca caaaatccga gtagttattt tgcctccccc ttcccattta acagaataca 31860 ccaatctctc cccacgcaca gctttaaaca tttggatacc attagatata gacatggttt 31920 tagattccac attccaaaca gtttcagagc gagccaatct ggggtcagtg atagataaaa 31980 atccatcggg atagtctttt aaagcgcttt cacagtccaa ctgctgcgga tgcgactccg 32040 gagtctggat cacggtcatc tggaagaaga acgatgggaa tcataatccg aaaacggtat 32100 cggacgattg tgtctcatca aacccacaag cagccgctgt ctgcgtcgct ccgtgcgact 32160 gctgtttatg ggatcagggt ccacagtgtc ctgaagcatg attttaatag cccttaacat 32220 caactttctg gtgcgatgcg cgcagcaacg cattctgatt tcactcaaat ctttgcagta 32280 ggtacaacac attattacaa tattgtttaa taaaccataa ttaaaagcgc tccagccaaa 32340 actcatatct gatataatcg cccctgcatg accatcatac caaagtttaa tataaattaa 32400 atgacgttcc ctcaaaaaca cactacccac atacatgatc tcttttggca tgtgcatatt 32460 aacaatctgt ctgtaccatg gacaacgttg gttaatcatg caacccaata taaccttccg 32520 gaaccacact gccaacaccg ctcccccagc catgcattga agtgaaccct gctgattaca 32580 atgacaatga agaacccaat tctctcgacc gtgaatcact tgagaatgaa aaatatctat 32640 agtggcacaa catagacata aatgcatgca tcttctcata atttttaact cctcaggatt 32700 tagaaacata tcccagggaa taggaagctc ttgcagaaca gtaaagctgg cagaacaagg 32760 aagaccacga acacaactta cactatgcat agtcatagta tcacaatctg gcaacagcgg 32820 gtggtcttca gtcatagaag ctcgggtttc attttcctca caacgtggta actgggctct 32880 ggtgtaaggg tgatgtctgg cgcatgatgt cgagcgtgcg cgcaaccttg tcataatgga 32940 gttgcttcct gacattctcg tattttgtat agcaaaacgc ggccctggca gaacacactc 33000 ttcttcgcct tctatcctgc cgcttagcgt gttccgtgtg atagttcaag tacaaccaca 33060 ctcttaagtt ggtcaaaaga atgctggctt cagttgtaat caaaactcca tcgcatctaa 33120 tcgttctgag gaaatcatcc aagcaatgca actggattgt gtttcaagca ggagaggaga 33180 gggaagagac ggaagaacca tgttaatttt tattccaaac gatctcgcag tacttcaaat 33240 tgtagatcgc gcagatggca tctctcgccc ccactgtgtt ggtgaaaaag cacagctaga 33300 tcaaaagaaa tgcgattttc aaggtgctca acggtggctt ccagcaaagc ctccacgcgc 33360 acatccaaga acaaaagaat accaaaagaa ggagcatttt ctaactcctc aatcatcata 33420 ttacattcct gcaccattcc cagataattt tcagctttcc agccttgaat tattcgtgtc 33480 agttcttgtg gtaaatccaa tccacacatt acaaacaggt cccggagggc gccctccacc 33540 accattctta aacacaccct cataatgaca aaatatcttg ctcctgtgtc acctgtagcg 33600 aattgagaat ggcaacatca attgacatgc ccttggctct aagttcttct ttaagttcta 33660 gttgtaaaaa ctctctcata ttatcaccaa actgcttagc cagaagcccc ccgggaacaa 33720 gagcagggga cgctacagtg cagtacaagc gcagacctcc ccaattggct ccagcaaaaa 33780 caagattgga ataagcatat tgggaaccgc cagtaatatc atcgaagttg ctggaaatat 33840 aatcaggcag agtttcttgt aaaaattgaa taaaagaaaa atttgccaaa aaaacattca 33900 aaacctctgg gatgcaaatg caataggtta ccgcgctgcg ctccaacatt gttagttttg 33960 aattagtctg caaaaataaa aaaaaaaaca agcgtcatat catagtagcc tgacgaacag 34020 atggataaat cagtctttcc atcacaagac aagccacagg gtctccagct cgaccctcgt 34080 aaaacctgtc atcatgatta aacaacagca ccgaaagttc ctcgcggtga ccagcatgaa 34140 taattcttga tgaagcatac aatccagaca tgttagcatc agttaacgag aaaaaacagc 34200 caacatagcc tttgggtata attatgctta atcgtaagta tagcaaagcc acccctcgcg 34260 gatacaaagt aaaaggcaca ggagaataaa aaatataatt atttctctgc tgctgttcag 34320 gcaacgtcgc ccccggtccc tctaaataca catacaaagc ctcatcagcc atggcttacc 34380 agacaaagta cagcgggcac acaaagcaca agctctaaag tgactctcca acctctccac 34440 aatatatata tacacaagcc ctaaactgac gtaatgggag taaagtgtaa aaaatcccgc 34500 caaacccaac acacaccccg aaactgcgtc accagggaaa agtacagttt cacttccgca 34560 atcccaacag gcgtaacttc ctctttctca cggtacgtga tatcccacta acttgcaacg 34620 tcattttccc acggtcgcac cgcccctttt agccgttaac cccacagcca atcaccacac 34680 gatccacact ttttaaaatc acctcattta catattggca ccattccatc tataaggtat 34740 attatataga taga 34754 <210> 5 <211> 33442 <212> DNA <213> Artificial Sequence <220> <223> NG-74 virus genome sequence <400> 5 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgctgctaa tcctttctct cttcaggcca ccatgggatg 29400 gagctgtatc atcctcttct tggtagcaac agctacaggt gtccactccg aggttcagct 29460 ggtggagtct ggcggtggcc tggtgcagcc agggggctca ctccgtttgt cctgtgcagc 29520 ttctggctat accttcacca actatggtat gaactgggtc cgtcaggccc cgggtaaggg 29580 cctggaatgg gttggatgga ttaacaccta taccggtgaa ccgacctatg ctgcggattt 29640 caaacgtcgt tttacttttt ctctggacac ctccaagagc acagcttacc tgcagatgaa 29700 cagcctgcgc gctgaggaca ctgccgtcta ttactgtgca aagtacccgc actattatgg 29760 gagcagccac tggtatttcg acgtctgggg tcaaggaacc ctggtcaccg tctcctcggg 29820 tggcggtggc tcgggcggtg gtggatctgg tggcggcgga tctgatatcc agatgaccca 29880 gtccccgagc tccctgtccg cctctgtggg cgatagggtc accatcacct gcagcgcaag 29940 tcaggatatt agcaactatt taaactggta tcaacagaaa ccaggaaaag ctccgaaagt 30000 cctgatttac ttcacctcct ctctccactc tggagtccct tctcgcttct ctggatccgg 30060 ttctgggacg gatttcactc tgaccatcag cagtctgcag ccagaagact tcgcaactta 30120 ttactgtcaa cagtatagca ccgtgccgtg gacgtttgga cagggtacca aggtggagat 30180 caaacgataa gctagcttga ctgactgaga tacagcgtac cttcagctca cagacatgat 30240 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 30300 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 30360 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 30420 ttaaagcaag taaaacctct acaaatgtgg tagtcgtcag ctatcctgca ggaacttgtt 30480 tatttgaaaa tcaattcaca aaatccgagt agttattttg cctccccctt cccatttaac 30540 agaatacacc aatctctccc cacgcacagc tttaaacatt tggataccat tagatataga 30600 catggtttta gattccacat tccaaacagt ttcagagcga gccaatctgg ggtcagtgat 30660 agataaaaat ccatcgggat agtcttttaa agcgctttca cagtccaact gctgcggatg 30720 cgactccgga gtctggatca cggtcatctg gaagaagaac gatgggaatc ataatccgaa 30780 aacggtatcg gacgattgtg tctcatcaaa cccacaagca gccgctgtct gcgtcgctcc 30840 gtgcgactgc tgtttatggg atcagggtcc acagtgtcct gaagcatgat tttaatagcc 30900 cttaacatca actttctggt gcgatgcgcg cagcaacgca ttctgatttc actcaaatct 30960 ttgcagtagg tacaacacat tattacaata ttgtttaata aaccataatt aaaagcgctc 31020 cagccaaaac tcatatctga tataatcgcc cctgcatgac catcatacca aagtttaata 31080 taaattaaat gacgttccct caaaaacaca ctacccacat acatgatctc ttttggcatg 31140 tgcatattaa caatctgtct gtaccatgga caacgttggt taatcatgca acccaatata 31200 accttccgga accacactgc caacaccgct cccccagcca tgcattgaag tgaaccctgc 31260 tgattacaat gacaatgaag aacccaattc tctcgaccgt gaatcacttg agaatgaaaa 31320 atatctatag tggcacaaca tagacataaa tgcatgcatc ttctcataat ttttaactcc 31380 tcaggattta gaaacatatc ccagggaata ggaagctctt gcagaacagt aaagctggca 31440 gaacaaggaa gaccacgaac acaacttaca ctatgcatag tcatagtatc acaatctggc 31500 aacagcgggt ggtcttcagt catagaagct cgggtttcat tttcctcaca acgtggtaac 31560 tgggctctgg tgtaagggtg atgtctggcg catgatgtcg agcgtgcgcg caaccttgtc 31620 ataatggagt tgcttcctga cattctcgta ttttgtatag caaaacgcgg ccctggcaga 31680 acacactctt cttcgccttc tatcctgccg cttagcgtgt tccgtgtgat agttcaagta 31740 caaccacact cttaagttgg tcaaaagaat gctggcttca gttgtaatca aaactccatc 31800 gcatctaatc gttctgagga aatcatccaa gcaatgcaac tggattgtgt ttcaagcagg 31860 agaggagagg gaagagacgg aagaaccatg ttaattttta ttccaaacga tctcgcagta 31920 cttcaaattg tagatcgcgc agatggcatc tctcgccccc actgtgttgg tgaaaaagca 31980 cagctagatc aaaagaaatg cgattttcaa ggtgctcaac ggtggcttcc agcaaagcct 32040 ccacgcgcac atccaagaac aaaagaatac caaaagaagg agcattttct aactcctcaa 32100 tcatcatatt acattcctgc accattccca gataattttc agctttccag ccttgaatta 32160 ttcgtgtcag ttcttgtggt aaatccaatc cacacattac aaacaggtcc cggagggcgc 32220 cctccaccac cattcttaaa cacaccctca taatgacaaa atatcttgct cctgtgtcac 32280 ctgtagcgaa ttgagaatgg caacatcaat tgacatgccc ttggctctaa gttcttcttt 32340 aagttctagt tgtaaaaact ctctcatatt atcaccaaac tgcttagcca gaagcccccc 32400 gggaacaaga gcaggggacg ctacagtgca gtacaagcgc agacctcccc aattggctcc 32460 agcaaaaaca agattggaat aagcatattg ggaaccgcca gtaatatcat cgaagttgct 32520 ggaaatataa tcaggcagag tttcttgtaa aaattgaata aaagaaaaat ttgccaaaaa 32580 aacattcaaa acctctggga tgcaaatgca ataggttacc gcgctgcgct ccaacattgt 32640 tagttttgaa ttagtctgca aaaataaaaa aaaaaacaag cgtcatatca tagtagcctg 32700 acgaacagat ggataaatca gtctttccat cacaagacaa gccacagggt ctccagctcg 32760 accctcgtaa aacctgtcat catgattaaa caacagcacc gaaagttcct cgcggtgacc 32820 agcatgaata attcttgatg aagcatacaa tccagacatg ttagcatcag ttaacgagaa 32880 aaaacagcca acatagcctt tgggtataat tatgcttaat cgtaagtata gcaaagccac 32940 ccctcgcgga tacaaagtaa aaggcacagg agaataaaaa atataattat ttctctgctg 33000 ctgttcaggc aacgtcgccc ccggtccctc taaatacaca tacaaagcct catcagccat 33060 ggcttaccag acaaagtaca gcgggcacac aaagcacaag ctctaaagtg actctccaac 33120 ctctccacaa tatatatata cacaagccct aaactgacgt aatgggagta aagtgtaaaa 33180 aatcccgcca aacccaacac acaccccgaa actgcgtcac cagggaaaag tacagtttca 33240 cttccgcaat cccaacaggc gtaacttcct ctttctcacg gtacgtgata tcccactaac 33300 ttgcaacgtc attttcccac ggtcgcaccg ccccttttag ccgttaaccc cacagccaat 33360 caccacacga tccacacttt ttaaaatcac ctcatttaca tattggcacc attccatcta 33420 taaggtatat tatatagata ga 33442 <210> 6 <211> 33460 <212> DNA <213> Artificial Sequence <220> <223> NG-78 virus genome sequence <400> 6 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgctgctaa tcctttctct cttcaggcca ccatgggatg 29400 gagctgtatc atcctcttct tggtagcaac agctacaggt gtccactccg aggttcagct 29460 ggtggagtct ggcggtggcc tggtgcagcc agggggctca ctccgtttgt cctgtgcagc 29520 ttctggctat accttcacca actatggtat gaactgggtc cgtcaggccc cgggtaaggg 29580 cctggaatgg gttggatgga ttaacaccta taccggtgaa ccgacctatg ctgcggattt 29640 caaacgtcgt tttacttttt ctctggacac ctccaagagc acagcttacc tgcagatgaa 29700 cagcctgcgc gctgaggaca ctgccgtcta ttactgtgca aagtacccgc actattatgg 29760 gagcagccac tggtatttcg acgtctgggg tcaaggaacc ctggtcaccg tctcctcggg 29820 tggcggtggc tcgggcggtg gtggatctgg tggcggcgga tctgatatcc agatgaccca 29880 gtccccgagc tccctgtccg cctctgtggg cgatagggtc accatcacct gcagcgcaag 29940 tcaggatatt agcaactatt taaactggta tcaacagaaa ccaggaaaag ctccgaaagt 30000 cctgatttac ttcacctcct ctctccactc tggagtccct tctcgcttct ctggatccgg 30060 ttctgggacg gatttcactc tgaccatcag cagtctgcag ccagaagact tcgcaactta 30120 ttactgtcaa cagtatagca ccgtgccgtg gacgtttgga cagggtacca aggtggagat 30180 caaacgacat catcaccacc accattaagc tagcttgact gactgagata cagcgtacct 30240 tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 30300 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 30360 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 30420 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta gtcgtcagct 30480 atcctgcagg aacttgttta tttgaaaatc aattcacaaa atccgagtag ttattttgcc 30540 tcccccttcc catttaacag aatacaccaa tctctcccca cgcacagctt taaacatttg 30600 gataccatta gatatagaca tggttttaga ttccacattc caaacagttt cagagcgagc 30660 caatctgggg tcagtgatag ataaaaatcc atcgggatag tcttttaaag cgctttcaca 30720 gtccaactgc tgcggatgcg actccggagt ctggatcacg gtcatctgga agaagaacga 30780 tgggaatcat aatccgaaaa cggtatcgga cgattgtgtc tcatcaaacc cacaagcagc 30840 cgctgtctgc gtcgctccgt gcgactgctg tttatgggat cagggtccac agtgtcctga 30900 agcatgattt taatagccct taacatcaac tttctggtgc gatgcgcgca gcaacgcatt 30960 ctgatttcac tcaaatcttt gcagtaggta caacacatta ttacaatatt gtttaataaa 31020 ccataattaa aagcgctcca gccaaaactc atatctgata taatcgcccc tgcatgacca 31080 tcataccaaa gtttaatata aattaaatga cgttccctca aaaacacact acccacatac 31140 atgatctctt ttggcatgtg catattaaca atctgtctgt accatggaca acgttggtta 31200 atcatgcaac ccaatataac cttccggaac cacactgcca acaccgctcc cccagccatg 31260 cattgaagtg aaccctgctg attacaatga caatgaagaa cccaattctc tcgaccgtga 31320 atcacttgag aatgaaaaat atctatagtg gcacaacata gacataaatg catgcatctt 31380 ctcataattt ttaactcctc aggatttaga aacatatccc agggaatagg aagctcttgc 31440 agaacagtaa agctggcaga acaaggaaga ccacgaacac aacttacact atgcatagtc 31500 atagtatcac aatctggcaa cagcgggtgg tcttcagtca tagaagctcg ggtttcattt 31560 tcctcacaac gtggtaactg ggctctggtg taagggtgat gtctggcgca tgatgtcgag 31620 cgtgcgcgca accttgtcat aatggagttg cttcctgaca ttctcgtatt ttgtatagca 31680 aaacgcggcc ctggcagaac acactcttct tcgccttcta tcctgccgct tagcgtgttc 31740 cgtgtgatag ttcaagtaca accacactct taagttggtc aaaagaatgc tggcttcagt 31800 tgtaatcaaa actccatcgc atctaatcgt tctgaggaaa tcatccaagc aatgcaactg 31860 gattgtgttt caagcaggag aggagaggga agagacggaa gaaccatgtt aatttttatt 31920 ccaaacgatc tcgcagtact tcaaattgta gatcgcgcag atggcatctc tcgcccccac 31980 tgtgttggtg aaaaagcaca gctagatcaa aagaaatgcg attttcaagg tgctcaacgg 32040 tggcttccag caaagcctcc acgcgcacat ccaagaacaa aagaatacca aaagaaggag 32100 cattttctaa ctcctcaatc atcatattac attcctgcac cattcccaga taattttcag 32160 ctttccagcc ttgaattatt cgtgtcagtt cttgtggtaa atccaatcca cacattacaa 32220 acaggtcccg gagggcgccc tccaccacca ttcttaaaca caccctcata atgacaaaat 32280 atcttgctcc tgtgtcacct gtagcgaatt gagaatggca acatcaattg acatgccctt 32340 ggctctaagt tcttctttaa gttctagttg taaaaactct ctcatattat caccaaactg 32400 cttagccaga agccccccgg gaacaagagc aggggacgct acagtgcagt acaagcgcag 32460 acctccccaa ttggctccag caaaaacaag attggaataa gcatattggg aaccgccagt 32520 aatatcatcg aagttgctgg aaatataatc aggcagagtt tcttgtaaaa attgaataaa 32580 agaaaaattt gccaaaaaaa cattcaaaac ctctgggatg caaatgcaat aggttaccgc 32640 gctgcgctcc aacattgtta gttttgaatt agtctgcaaa aataaaaaaa aaaacaagcg 32700 tcatatcata gtagcctgac gaacagatgg ataaatcagt ctttccatca caagacaagc 32760 cacagggtct ccagctcgac cctcgtaaaa cctgtcatca tgattaaaca acagcaccga 32820 aagttcctcg cggtgaccag catgaataat tcttgatgaa gcatacaatc cagacatgtt 32880 agcatcagtt aacgagaaaa aacagccaac atagcctttg ggtataatta tgcttaatcg 32940 taagtatagc aaagccaccc ctcgcggata caaagtaaaa ggcacaggag aataaaaaat 33000 ataattattt ctctgctgct gttcaggcaa cgtcgccccc ggtccctcta aatacacata 33060 caaagcctca tcagccatgg cttaccagac aaagtacagc gggcacacaa agcacaagct 33120 ctaaagtgac tctccaacct ctccacaata tatatataca caagccctaa actgacgtaa 33180 tgggagtaaa gtgtaaaaaa tcccgccaaa cccaacacac accccgaaac tgcgtcacca 33240 gggaaaagta cagtttcact tccgcaatcc caacaggcgt aacttcctct ttctcacggt 33300 acgtgatatc ccactaactt gcaacgtcat tttcccacgg tcgcaccgcc ccttttagcc 33360 gttaacccca cagccaatca ccacacgatc cacacttttt aaaatcacct catttacata 33420 ttggcaccat tccatctata aggtatatta tatagataga 33460 <210> 7 <211> 34117 <212> DNA <213> Artificial Sequence <220> <223> NG-76 virus genome sequence <400> 7 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgctgc caagcttccg agctctcgaa ttcaaaggag gtacccacca tgggatggag 30060 ctgtatcatc ctcttcttgg tagcaacagc tacaggtgtc cactccgagg ttcagctggt 30120 ggagtctggc ggtggcctgg tgcagccagg gggctcactc cgtttgtcct gtgcagcttc 30180 tggctatacc ttcaccaact atggtatgaa ctgggtccgt caggccccgg gtaagggcct 30240 ggaatgggtt ggatggatta acacctatac cggtgaaccg acctatgctg cggatttcaa 30300 acgtcgtttt actttttctc tggacacctc caagagcaca gcttacctgc agatgaacag 30360 cctgcgcgct gaggacactg ccgtctatta ctgtgcaaag tacccgcact attatgggag 30420 cagccactgg tatttcgacg tctggggtca aggaaccctg gtcaccgtct cctcgggtgg 30480 cggtggctcg ggcggtggtg gatctggtgg cggcggatct gatatccaga tgacccagtc 30540 cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc atcacctgca gcgcaagtca 30600 ggatattagc aactatttaa actggtatca acagaaacca ggaaaagctc cgaaagtcct 30660 gatttacttc acctcctctc tccactctgg agtcccttct cgcttctctg gatccggttc 30720 tgggacggat ttcactctga ccatcagcag tctgcagcca gaagacttcg caacttatta 30780 ctgtcaacag tatagcaccg tgccgtggac gtttggacag ggtaccaagg tggagatcaa 30840 acgacatcat caccaccacc attaagctag cttgactgac tgagatacag cgtaccttca 30900 gctcacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 30960 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 31020 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 31080 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtagtc gtcagctatc 31140 ctgcaggaac ttgtttattt gaaaatcaat tcacaaaatc cgagtagtta ttttgcctcc 31200 cccttcccat ttaacagaat acaccaatct ctccccacgc acagctttaa acatttggat 31260 accattagat atagacatgg ttttagattc cacattccaa acagtttcag agcgagccaa 31320 tctggggtca gtgatagata aaaatccatc gggatagtct tttaaagcgc tttcacagtc 31380 caactgctgc ggatgcgact ccggagtctg gatcacggtc atctggaaga agaacgatgg 31440 gaatcataat ccgaaaacgg tatcggacga ttgtgtctca tcaaacccac aagcagccgc 31500 tgtctgcgtc gctccgtgcg actgctgttt atgggatcag ggtccacagt gtcctgaagc 31560 atgattttaa tagcccttaa catcaacttt ctggtgcgat gcgcgcagca acgcattctg 31620 atttcactca aatctttgca gtaggtacaa cacattatta caatattgtt taataaacca 31680 taattaaaag cgctccagcc aaaactcata tctgatataa tcgcccctgc atgaccatca 31740 taccaaagtt taatataaat taaatgacgt tccctcaaaa acacactacc cacatacatg 31800 atctcttttg gcatgtgcat attaacaatc tgtctgtacc atggacaacg ttggttaatc 31860 atgcaaccca atataacctt ccggaaccac actgccaaca ccgctccccc agccatgcat 31920 tgaagtgaac cctgctgatt acaatgacaa tgaagaaccc aattctctcg accgtgaatc 31980 acttgagaat gaaaaatatc tatagtggca caacatagac ataaatgcat gcatcttctc 32040 ataattttta actcctcagg atttagaaac atatcccagg gaataggaag ctcttgcaga 32100 acagtaaagc tggcagaaca aggaagacca cgaacacaac ttacactatg catagtcata 32160 gtatcacaat ctggcaacag cgggtggtct tcagtcatag aagctcgggt ttcattttcc 32220 tcacaacgtg gtaactgggc tctggtgtaa gggtgatgtc tggcgcatga tgtcgagcgt 32280 gcgcgcaacc ttgtcataat ggagttgctt cctgacattc tcgtattttg tatagcaaaa 32340 cgcggccctg gcagaacaca ctcttcttcg ccttctatcc tgccgcttag cgtgttccgt 32400 gtgatagttc aagtacaacc acactcttaa gttggtcaaa agaatgctgg cttcagttgt 32460 aatcaaaact ccatcgcatc taatcgttct gaggaaatca tccaagcaat gcaactggat 32520 tgtgtttcaa gcaggagagg agagggaaga gacggaagaa ccatgttaat ttttattcca 32580 aacgatctcg cagtacttca aattgtagat cgcgcagatg gcatctctcg cccccactgt 32640 gttggtgaaa aagcacagct agatcaaaag aaatgcgatt ttcaaggtgc tcaacggtgg 32700 cttccagcaa agcctccacg cgcacatcca agaacaaaag aataccaaaa gaaggagcat 32760 tttctaactc ctcaatcatc atattacatt cctgcaccat tcccagataa ttttcagctt 32820 tccagccttg aattattcgt gtcagttctt gtggtaaatc caatccacac attacaaaca 32880 ggtcccggag ggcgccctcc accaccattc ttaaacacac cctcataatg acaaaatatc 32940 ttgctcctgt gtcacctgta gcgaattgag aatggcaaca tcaattgaca tgcccttggc 33000 tctaagttct tctttaagtt ctagttgtaa aaactctctc atattatcac caaactgctt 33060 agccagaagc cccccgggaa caagagcagg ggacgctaca gtgcagtaca agcgcagacc 33120 tccccaattg gctccagcaa aaacaagatt ggaataagca tattgggaac cgccagtaat 33180 atcatcgaag ttgctggaaa tataatcagg cagagtttct tgtaaaaatt gaataaaaga 33240 aaaatttgcc aaaaaaacat tcaaaacctc tgggatgcaa atgcaatagg ttaccgcgct 33300 gcgctccaac attgttagtt ttgaattagt ctgcaaaaat aaaaaaaaaa acaagcgtca 33360 tatcatagta gcctgacgaa cagatggata aatcagtctt tccatcacaa gacaagccac 33420 agggtctcca gctcgaccct cgtaaaacct gtcatcatga ttaaacaaca gcaccgaaag 33480 ttcctcgcgg tgaccagcat gaataattct tgatgaagca tacaatccag acatgttagc 33540 atcagttaac gagaaaaaac agccaacata gcctttgggt ataattatgc ttaatcgtaa 33600 gtatagcaaa gccacccctc gcggatacaa agtaaaaggc acaggagaat aaaaaatata 33660 attatttctc tgctgctgtt caggcaacgt cgcccccggt ccctctaaat acacatacaa 33720 agcctcatca gccatggctt accagacaaa gtacagcggg cacacaaagc acaagctcta 33780 aagtgactct ccaacctctc cacaatatat atatacacaa gccctaaact gacgtaatgg 33840 gagtaaagtg taaaaaatcc cgccaaaccc aacacacacc ccgaaactgc gtcaccaggg 33900 aaaagtacag tttcacttcc gcaatcccaa caggcgtaac ttcctctttc tcacggtacg 33960 tgatatccca ctaacttgca acgtcatttt cccacggtcg caccgcccct tttagccgtt 34020 aaccccacag ccaatcacca cacgatccac actttttaaa atcacctcat ttacatattg 34080 gcaccattcc atctataagg tatattatat agataga 34117 <210> 8 <211> 34099 <212> DNA <213> Artificial Sequence <220> <223> NG-73 virus genome sequence <400> 8 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgctgc caagcttccg agctctcgaa ttcaaaggag gtacccacca tgggatggag 30060 ctgtatcatc ctcttcttgg tagcaacagc tacaggtgtc cactccgagg ttcagctggt 30120 ggagtctggc ggtggcctgg tgcagccagg gggctcactc cgtttgtcct gtgcagcttc 30180 tggctatacc ttcaccaact atggtatgaa ctgggtccgt caggccccgg gtaagggcct 30240 ggaatgggtt ggatggatta acacctatac cggtgaaccg acctatgctg cggatttcaa 30300 acgtcgtttt actttttctc tggacacctc caagagcaca gcttacctgc agatgaacag 30360 cctgcgcgct gaggacactg ccgtctatta ctgtgcaaag tacccgcact attatgggag 30420 cagccactgg tatttcgacg tctggggtca aggaaccctg gtcaccgtct cctcgggtgg 30480 cggtggctcg ggcggtggtg gatctggtgg cggcggatct gatatccaga tgacccagtc 30540 cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc atcacctgca gcgcaagtca 30600 ggatattagc aactatttaa actggtatca acagaaacca ggaaaagctc cgaaagtcct 30660 gatttacttc acctcctctc tccactctgg agtcccttct cgcttctctg gatccggttc 30720 tgggacggat ttcactctga ccatcagcag tctgcagcca gaagacttcg caacttatta 30780 ctgtcaacag tatagcaccg tgccgtggac gtttggacag ggtaccaagg tggagatcaa 30840 acgataagct agcttgactg actgagatac agcgtacctt cagctcacag acatgataag 30900 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg 30960 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa 31020 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta 31080 aagcaagtaa aacctctaca aatgtggtag tcgtcagcta tcctgcagga acttgtttat 31140 ttgaaaatca attcacaaaa tccgagtagt tattttgcct cccccttccc atttaacaga 31200 atacaccaat ctctccccac gcacagcttt aaacatttgg ataccattag atatagacat 31260 ggttttagat tccacattcc aaacagtttc agagcgagcc aatctggggt cagtgataga 31320 taaaaatcca tcgggatagt cttttaaagc gctttcacag tccaactgct gcggatgcga 31380 ctccggagtc tggatcacgg tcatctggaa gaagaacgat gggaatcata atccgaaaac 31440 ggtatcggac gattgtgtct catcaaaccc acaagcagcc gctgtctgcg tcgctccgtg 31500 cgactgctgt ttatgggatc agggtccaca gtgtcctgaa gcatgatttt aatagccctt 31560 aacatcaact ttctggtgcg atgcgcgcag caacgcattc tgatttcact caaatctttg 31620 cagtaggtac aacacattat tacaatattg tttaataaac cataattaaa agcgctccag 31680 ccaaaactca tatctgatat aatcgcccct gcatgaccat cataccaaag tttaatataa 31740 attaaatgac gttccctcaa aaacacacta cccacataca tgatctcttt tggcatgtgc 31800 atattaacaa tctgtctgta ccatggacaa cgttggttaa tcatgcaacc caatataacc 31860 ttccggaacc acactgccaa caccgctccc ccagccatgc attgaagtga accctgctga 31920 ttacaatgac aatgaagaac ccaattctct cgaccgtgaa tcacttgaga atgaaaaata 31980 tctatagtgg cacaacatag acataaatgc atgcatcttc tcataatttt taactcctca 32040 ggatttagaa acatatccca gggaatagga agctcttgca gaacagtaaa gctggcagaa 32100 caaggaagac cacgaacaca acttacacta tgcatagtca tagtatcaca atctggcaac 32160 agcgggtggt cttcagtcat agaagctcgg gtttcatttt cctcacaacg tggtaactgg 32220 gctctggtgt aagggtgatg tctggcgcat gatgtcgagc gtgcgcgcaa ccttgtcata 32280 atggagttgc ttcctgacat tctcgtattt tgtatagcaa aacgcggccc tggcagaaca 32340 cactcttctt cgccttctat cctgccgctt agcgtgttcc gtgtgatagt tcaagtacaa 32400 ccacactctt aagttggtca aaagaatgct ggcttcagtt gtaatcaaaa ctccatcgca 32460 tctaatcgtt ctgaggaaat catccaagca atgcaactgg attgtgtttc aagcaggaga 32520 ggagagggaa gagacggaag aaccatgtta atttttattc caaacgatct cgcagtactt 32580 caaattgtag atcgcgcaga tggcatctct cgcccccact gtgttggtga aaaagcacag 32640 ctagatcaaa agaaatgcga ttttcaaggt gctcaacggt ggcttccagc aaagcctcca 32700 cgcgcacatc caagaacaaa agaataccaa aagaaggagc attttctaac tcctcaatca 32760 tcatattaca ttcctgcacc attcccagat aattttcagc tttccagcct tgaattattc 32820 gtgtcagttc ttgtggtaaa tccaatccac acattacaaa caggtcccgg agggcgccct 32880 ccaccaccat tcttaaacac accctcataa tgacaaaata tcttgctcct gtgtcacctg 32940 tagcgaattg agaatggcaa catcaattga catgcccttg gctctaagtt cttctttaag 33000 ttctagttgt aaaaactctc tcatattatc accaaactgc ttagccagaa gccccccggg 33060 aacaagagca ggggacgcta cagtgcagta caagcgcaga cctccccaat tggctccagc 33120 aaaaacaaga ttggaataag catattggga accgccagta atatcatcga agttgctgga 33180 aatataatca ggcagagttt cttgtaaaaa ttgaataaaa gaaaaatttg ccaaaaaaac 33240 attcaaaacc tctgggatgc aaatgcaata ggttaccgcg ctgcgctcca acattgttag 33300 ttttgaatta gtctgcaaaa ataaaaaaaa aaacaagcgt catatcatag tagcctgacg 33360 aacagatgga taaatcagtc tttccatcac aagacaagcc acagggtctc cagctcgacc 33420 ctcgtaaaac ctgtcatcat gattaaacaa cagcaccgaa agttcctcgc ggtgaccagc 33480 atgaataatt cttgatgaag catacaatcc agacatgtta gcatcagtta acgagaaaaa 33540 acagccaaca tagcctttgg gtataattat gcttaatcgt aagtatagca aagccacccc 33600 tcgcggatac aaagtaaaag gcacaggaga ataaaaaata taattatttc tctgctgctg 33660 ttcaggcaac gtcgcccccg gtccctctaa atacacatac aaagcctcat cagccatggc 33720 ttaccagaca aagtacagcg ggcacacaaa gcacaagctc taaagtgact ctccaacctc 33780 tccacaatat atatatacac aagccctaaa ctgacgtaat gggagtaaag tgtaaaaaat 33840 cccgccaaac ccaacacaca ccccgaaact gcgtcaccag ggaaaagtac agtttcactt 33900 ccgcaatccc aacaggcgta acttcctctt tctcacggta cgtgatatcc cactaacttg 33960 caacgtcatt ttcccacggt cgcaccgccc cttttagccg ttaaccccac agccaatcac 34020 cacacgatcc acacttttta aaatcacctc atttacatat tggcaccatt ccatctataa 34080 ggtatattat atagataga 34099 <210> 9 <211> 36030 <212> DNA <213> Artificial Sequence <220> <223> NG-134 virus genome sequence <400> 9 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgctgc caagcttccg agctctcgaa ttcaaaggag gtacccacca tgggatggag 30060 ctgtatcatc ctcttcttgg tagcaacagc tacaggtgtc cactccgagg ttcagctggt 30120 ggagtctggc ggtggcctgg tgcagccagg gggctcactc cgtttgtcct gtgcagcttc 30180 tggctatacc ttcaccaact atggtatgaa ctgggtccgt caggccccgg gtaagggcct 30240 ggaatgggtt ggatggatta acacctatac cggtgaaccg acctatgctg cggatttcaa 30300 acgtcgtttt actttttctc tggacacctc caagagcaca gcttacctgc agatgaacag 30360 cctgcgcgct gaggacactg ccgtctatta ctgtgcaaag tacccgcact attatgggag 30420 cagccactgg tatttcgacg tctggggtca aggaaccctg gtcaccgtct cctcggcctc 30480 caccaagggc ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac 30540 agcggccctg ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa 30600 ctcaggcgcc ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact 30660 ctactccctc agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat 30720 ctgcaacgtg aatcacaagc ccagcaacac caaggtcgac aagaaagttg agcccaaatc 30780 ttgtgacaaa actcacacat gcccaccgtg cccagcacct gagctcctgg ggggaccgtc 30840 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt 30900 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt 30960 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac 31020 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta 31080 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc 31140 caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg aggagatgac 31200 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt 31260 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga 31320 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca 31380 ggggaacgtc ttctcatgct ccgtgatgca cgaggctctg cacaaccact acacgcagaa 31440 gagcctctcc ctgtctccgg gtaaatgatc tagactcgag gtttctagaa gttgtctcct 31500 cctgcactga ctgactgacc taacgttact ggccgaagcc gcttggaata aggccggtgt 31560 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg 31620 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga 31680 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa 31740 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 31800 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac 31860 gttgtgagtt ggatagttgt ggaaagagtc aaatggctcc cctcaagcgt attcaacaag 31920 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc 31980 acatgctttt catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg 32040 acgtggtttt cctttgaaaa acacgatgat aataacatgg actggacctg gcgcgtgttt 32100 tgcctgctcg ccgtggctcc tggggcccac agcgatatcc agatgaccca gtccccgagc 32160 tccctgtccg cctctgtggg cgatagggtc accatcacct gcagcgcaag tcaggatatt 32220 agcaactatt taaactggta tcaacagaaa ccaggaaaag ctccgaaagt cctgatttac 32280 ttcacctcct ctctccactc tggagtccct tctcgcttct ctggatccgg ttctgggacg 32340 gatttcactc tgaccatcag cagtctgcag ccagaagact tcgcaactta ttactgtcaa 32400 cagtatagca ccgtgccgtg gacgtttgga cagggtacca aggtggagat caaacgaact 32460 gtggctgcac catctgtctt catcttcccg ccatctgatg agcagttgaa atctggaact 32520 gcttctgttg tgtgcctgct gaataacttc tatcccagag aggccaaagt acagtggaag 32580 gtggataacg ccctccaatc gggtaactcc caggagagtg tcacagagca ggacagcaag 32640 gacagcacct acagcctcag cagcaccctg acgctgagca aagcagacta cgagaaacac 32700 aaagtctacg cctgcgaagt cacccatcag ggcctgagct cgcccgtcac aaagagcttc 32760 aacaggggag agtgttaagc tagcttgact gactgagata cagcgtacct tcagctcaca 32820 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 32880 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 32940 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 33000 gaggtttttt aaagcaagta aaacctctac aaatgtggta gtcgtcagct atcctgcagg 33060 aacttgttta tttgaaaatc aattcacaaa atccgagtag ttattttgcc tcccccttcc 33120 catttaacag aatacaccaa tctctcccca cgcacagctt taaacatttg gataccatta 33180 gatatagaca tggttttaga ttccacattc caaacagttt cagagcgagc caatctgggg 33240 tcagtgatag ataaaaatcc atcgggatag tcttttaaag cgctttcaca gtccaactgc 33300 tgcggatgcg actccggagt ctggatcacg gtcatctgga agaagaacga tgggaatcat 33360 aatccgaaaa cggtatcgga cgattgtgtc tcatcaaacc cacaagcagc cgctgtctgc 33420 gtcgctccgt gcgactgctg tttatgggat cagggtccac agtgtcctga agcatgattt 33480 taatagccct taacatcaac tttctggtgc gatgcgcgca gcaacgcatt ctgatttcac 33540 tcaaatcttt gcagtaggta caacacatta ttacaatatt gtttaataaa ccataattaa 33600 aagcgctcca gccaaaactc atatctgata taatcgcccc tgcatgacca tcataccaaa 33660 gtttaatata aattaaatga cgttccctca aaaacacact acccacatac atgatctctt 33720 ttggcatgtg catattaaca atctgtctgt accatggaca acgttggtta atcatgcaac 33780 ccaatataac cttccggaac cacactgcca acaccgctcc cccagccatg cattgaagtg 33840 aaccctgctg attacaatga caatgaagaa cccaattctc tcgaccgtga atcacttgag 33900 aatgaaaaat atctatagtg gcacaacata gacataaatg catgcatctt ctcataattt 33960 ttaactcctc aggatttaga aacatatccc agggaatagg aagctcttgc agaacagtaa 34020 agctggcaga acaaggaaga ccacgaacac aacttacact atgcatagtc atagtatcac 34080 aatctggcaa cagcgggtgg tcttcagtca tagaagctcg ggtttcattt tcctcacaac 34140 gtggtaactg ggctctggtg taagggtgat gtctggcgca tgatgtcgag cgtgcgcgca 34200 accttgtcat aatggagttg cttcctgaca ttctcgtatt ttgtatagca aaacgcggcc 34260 ctggcagaac acactcttct tcgccttcta tcctgccgct tagcgtgttc cgtgtgatag 34320 ttcaagtaca accacactct taagttggtc aaaagaatgc tggcttcagt tgtaatcaaa 34380 actccatcgc atctaatcgt tctgaggaaa tcatccaagc aatgcaactg gattgtgttt 34440 caagcaggag aggagaggga agagacggaa gaaccatgtt aatttttatt ccaaacgatc 34500 tcgcagtact tcaaattgta gatcgcgcag atggcatctc tcgcccccac tgtgttggtg 34560 aaaaagcaca gctagatcaa aagaaatgcg attttcaagg tgctcaacgg tggcttccag 34620 caaagcctcc acgcgcacat ccaagaacaa aagaatacca aaagaaggag cattttctaa 34680 ctcctcaatc atcatattac attcctgcac cattcccaga taattttcag ctttccagcc 34740 ttgaattatt cgtgtcagtt cttgtggtaa atccaatcca cacattacaa acaggtcccg 34800 gagggcgccc tccaccacca ttcttaaaca caccctcata atgacaaaat atcttgctcc 34860 tgtgtcacct gtagcgaatt gagaatggca acatcaattg acatgccctt ggctctaagt 34920 tcttctttaa gttctagttg taaaaactct ctcatattat caccaaactg cttagccaga 34980 agccccccgg gaacaagagc aggggacgct acagtgcagt acaagcgcag acctccccaa 35040 ttggctccag caaaaacaag attggaataa gcatattggg aaccgccagt aatatcatcg 35100 aagttgctgg aaatataatc aggcagagtt tcttgtaaaa attgaataaa agaaaaattt 35160 gccaaaaaaa cattcaaaac ctctgggatg caaatgcaat aggttaccgc gctgcgctcc 35220 aacattgtta gttttgaatt agtctgcaaa aataaaaaaa aaaacaagcg tcatatcata 35280 gtagcctgac gaacagatgg ataaatcagt ctttccatca caagacaagc cacagggtct 35340 ccagctcgac cctcgtaaaa cctgtcatca tgattaaaca acagcaccga aagttcctcg 35400 cggtgaccag catgaataat tcttgatgaa gcatacaatc cagacatgtt agcatcagtt 35460 aacgagaaaa aacagccaac atagcctttg ggtataatta tgcttaatcg taagtatagc 35520 aaagccaccc ctcgcggata caaagtaaaa ggcacaggag aataaaaaat ataattattt 35580 ctctgctgct gttcaggcaa cgtcgccccc ggtccctcta aatacacata caaagcctca 35640 tcagccatgg cttaccagac aaagtacagc gggcacacaa agcacaagct ctaaagtgac 35700 tctccaacct ctccacaata tatatataca caagccctaa actgacgtaa tgggagtaaa 35760 gtgtaaaaaa tcccgccaaa cccaacacac accccgaaac tgcgtcacca gggaaaagta 35820 cagtttcact tccgcaatcc caacaggcgt aacttcctct ttctcacggt acgtgatatc 35880 ccactaactt gcaacgtcat tttcccacgg tcgcaccgcc ccttttagcc gttaacccca 35940 cagccaatca ccacacgatc cacacttttt aaaatcacct catttacata ttggcaccat 36000 tccatctata aggtatatta tatagataga 36030 <210> 10 <211> 201 <212> DNA <213> Artificial Sequence <220> <223> BX DNA sequence corresponding to and including bp 28166-28366 of the EnAd genome <400> 10 aaaatgatta ataaaaaatc acttacttga aatcagcaat aaggtctctg ttgaaatttt 60 ctcccagcag cacctcactt ccctcttccc aactctggta ttctaaaccc cgttcagcgg 120 catactttct ccatacttta aaggggatgt caaattttag ctcctctcct gtacccacaa 180 tcttcatgtc tttcttccca g 201 <210> 11 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> BY DNA sequence corresponding to and including bp 29345-29379 of the EnAd genome <400> 11 caaataaagt ttaacttgtt tatttgaaaa tcaat 35 <210> 12 <211> 32326 <212> DNA <213> Artificial Sequence <220> <223> EnAd genome <400> 12 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttaact tgtttatttg aaaatcaatt cacaaaatcc gagtagttat 29400 tttgcctccc ccttcccatt taacagaata caccaatctc tccccacgca cagctttaaa 29460 catttggata ccattagata tagacatggt tttagattcc acattccaaa cagtttcaga 29520 gcgagccaat ctggggtcag tgatagataa aaatccatcg ggatagtctt ttaaagcgct 29580 ttcacagtcc aactgctgcg gatgcgactc cggagtctgg atcacggtca tctggaagaa 29640 gaacgatggg aatcataatc cgaaaacggt atcggacgat tgtgtctcat caaacccaca 29700 agcagccgct gtctgcgtcg ctccgtgcga ctgctgttta tgggatcagg gtccacagtg 29760 tcctgaagca tgattttaat agcccttaac atcaactttc tggtgcgatg cgcgcagcaa 29820 cgcattctga tttcactcaa atctttgcag taggtacaac acattattac aatattgttt 29880 aataaaccat aattaaaagc gctccagcca aaactcatat ctgatataat cgcccctgca 29940 tgaccatcat accaaagttt aatataaatt aaatgacgtt ccctcaaaaa cacactaccc 30000 acatacatga tctcttttgg catgtgcata ttaacaatct gtctgtacca tggacaacgt 30060 tggttaatca tgcaacccaa tataaccttc cggaaccaca ctgccaacac cgctccccca 30120 gccatgcatt gaagtgaacc ctgctgatta caatgacaat gaagaaccca attctctcga 30180 ccgtgaatca cttgagaatg aaaaatatct atagtggcac aacatagaca taaatgcatg 30240 catcttctca taatttttaa ctcctcagga tttagaaaca tatcccaggg aataggaagc 30300 tcttgcagaa cagtaaagct ggcagaacaa ggaagaccac gaacacaact tacactatgc 30360 atagtcatag tatcacaatc tggcaacagc gggtggtctt cagtcataga agctcgggtt 30420 tcattttcct cacaacgtgg taactgggct ctggtgtaag ggtgatgtct ggcgcatgat 30480 gtcgagcgtg cgcgcaacct tgtcataatg gagttgcttc ctgacattct cgtattttgt 30540 atagcaaaac gcggccctgg cagaacacac tcttcttcgc cttctatcct gccgcttagc 30600 gtgttccgtg tgatagttca agtacaacca cactcttaag ttggtcaaaa gaatgctggc 30660 ttcagttgta atcaaaactc catcgcatct aatcgttctg aggaaatcat ccaagcaatg 30720 caactggatt gtgtttcaag caggagagga gagggaagag acggaagaac catgttaatt 30780 tttattccaa acgatctcgc agtacttcaa attgtagatc gcgcagatgg catctctcgc 30840 ccccactgtg ttggtgaaaa agcacagcta gatcaaaaga aatgcgattt tcaaggtgct 30900 caacggtggc ttccagcaaa gcctccacgc gcacatccaa gaacaaaaga ataccaaaag 30960 aaggagcatt ttctaactcc tcaatcatca tattacattc ctgcaccatt cccagataat 31020 tttcagcttt ccagccttga attattcgtg tcagttcttg tggtaaatcc aatccacaca 31080 ttacaaacag gtcccggagg gcgccctcca ccaccattct taaacacacc ctcataatga 31140 caaaatatct tgctcctgtg tcacctgtag cgaattgaga atggcaacat caattgacat 31200 gcccttggct ctaagttctt ctttaagttc tagttgtaaa aactctctca tattatcacc 31260 aaactgctta gccagaagcc ccccgggaac aagagcaggg gacgctacag tgcagtacaa 31320 gcgcagacct ccccaattgg ctccagcaaa aacaagattg gaataagcat attgggaacc 31380 gccagtaata tcatcgaagt tgctggaaat ataatcaggc agagtttctt gtaaaaattg 31440 aataaaagaa aaatttgcca aaaaaacatt caaaacctct gggatgcaaa tgcaataggt 31500 taccgcgctg cgctccaaca ttgttagttt tgaattagtc tgcaaaaata aaaaaaaaaa 31560 caagcgtcat atcatagtag cctgacgaac agatggataa atcagtcttt ccatcacaag 31620 acaagccaca gggtctccag ctcgaccctc gtaaaacctg tcatcatgat taaacaacag 31680 caccgaaagt tcctcgcggt gaccagcatg aataattctt gatgaagcat acaatccaga 31740 catgttagca tcagttaacg agaaaaaaca gccaacatag cctttgggta taattatgct 31800 taatcgtaag tatagcaaag ccacccctcg cggatacaaa gtaaaaggca caggagaata 31860 aaaaatataa ttatttctct gctgctgttc aggcaacgtc gcccccggtc cctctaaata 31920 cacatacaaa gcctcatcag ccatggctta ccagacaaag tacagcgggc acacaaagca 31980 caagctctaa agtgactctc caacctctcc acaatatata tatacacaag ccctaaactg 32040 acgtaatggg agtaaagtgt aaaaaatccc gccaaaccca acacacaccc cgaaactgcg 32100 tcaccaggga aaagtacagt ttcacttccg caatcccaac aggcgtaact tcctctttct 32160 cacggtacgt gatatcccac taacttgcaa cgtcattttc ccacggtcgc accgcccctt 32220 ttagccgtta accccacagc caatcaccac acgatccaca ctttttaaaa tcacctcatt 32280 tacatattgg caccattcca tctataaggt atattatata gataga 32326 <210> 13 <211> 572 <212> DNA <213> Artificial Sequence <220> <223> CMV exogenous promoter <400> 13 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 60 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 120 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 180 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 240 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 300 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc 360 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 420 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 480 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 540 tctatataag cagagctggt ttagtgaacc gt 572 <210> 14 <211> 505 <212> DNA <213> Artificial Sequence <220> <223> PGK exogenous promoter <400> 14 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 60 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 120 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta cccctcccct 180 agtcaggaag ttcccccccg ccccgcagct cgcgtcatgc aggacgtgac aaatggaagt 240 agcacgtctc actagtctcg tgcaaatgga cagcaccgct gagcaatgga agcgggtagg 300 cccttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 360 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 420 gtcctccgga ggcccggcat tccgcacgct tcaaaagcgc acgtctgccg cgctgttctc 480 ttcttcctca tctccgggcc tttcg 505 <210> 15 <211> 367 <212> DNA <213> Artificial Sequence <220> <223> CBA exogenous promoter <400> 15 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca 60 attttgtatt tatttatttt ttaattattt tatgcagcga tgggggcggg gggggggggg 120 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc 180 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 240 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc 300 ccgtgccccg ctccgcgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact 360 cccacag 367 <210> 16 <211> 4 <212> DNA <213> Artificial Sequence <220> <223> Short splice acceptor (SSA) <400> 16 000 4 <210> 17 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> splice acceptor (SA) <400> 17 cctttctctc ttcagg 16 <210> 18 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> branched splice acceptor (bSA) <400> 18 tgctaatcct ttctctcttc agg 23 <210> 19 <211> 551 <212> DNA <213> Artificial Sequence <220> <223> Internal Ribosome Entry sequence (IRES) <400> 19 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 60 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 120 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 180 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 240 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 300 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 360 aagagtcaaa tggctcccct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 420 accccattgt atgggatctg atctggggcc tcggtgcaca tgcttttcat gtgtttagtc 480 gaggttaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca 540 cgatgataat a 551 <210> 20 <211> 192 <212> DNA <213> Artificial Sequence <220> <223> polyadenylation sequence <400> 20 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 60 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 120 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 180 gggaggtttt tt 192 <210> 21 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Leader sequence <400> 21 Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 1 5 10 15 Val His Ser <210> 22 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Leader sequence <400> 22 Met Asp Trp Thr Trp Arg Val Phe Cys Leu Leu Ala Val Ala Pro Gly 1 5 10 15 Ala His Ser <210> 23 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Histidine tag <400> 23 His His His His His His 1 5 <210> 24 <211> 14 <212> PRT <213> Artificial Sequence <220> <223> V5 tag <400> 24 Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 1 5 10 <210> 25 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> P2A peptide <400> 25 Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 1 5 10 15 Glu Glu Asn Pro Gly Pro 20 <210> 26 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> F2A peptide <400> 26 Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 1 5 10 15 Glu Asn Pro Gly Pro 20 <210> 27 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> E2A peptide <400> 27 Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 1 5 10 15 Val Glu Ser Asn Pro Gly Pro 20 <210> 28 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> T2A peptide <400> 28 Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 1 5 10 15 Gly Asp Val Glu Ser Asn Pro Gly Pro 20 25 <210> 29 <211> 123 <212> PRT <213> Artificial Sequence <220> <223> anti-VEGF ab VH chain amino acid sequence <400> 29 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 20 25 30 Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 50 55 60 Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 100 105 110 Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> 30 <211> 118 <212> PRT <213> Artificial Sequence <220> <223> anti-PD-L1 antibody VH chain amino acid sequence <400> 30 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 20 25 30 Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 50 55 60 Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 100 105 110 Leu Val Thr Val Ser Ala 115 <210> 31 <211> 108 <212> PRT <213> Artificial Sequence <220> <223> anti-VEGF ab VL chain amino acid sequence <400> 31 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 35 40 45 Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 100 105 <210> 32 <211> 108 <212> PRT <213> Artificial Sequence <220> <223> anti-PD-L1 antibody VL chain amino acid sequence <400> 32 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 20 25 30 Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 100 105 <210> 33 <211> 330 <212> PRT <213> Artificial Sequence <220> <223> human IgG1 constant heavy chain amino acid sequence <400> 33 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235 240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> 34 <211> 330 <212> PRT <213> Artificial Sequence <220> <223> human IgG1 modified constant heavy chain amino acid sequence <400> 34 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 130 135 140 Val Val Val Ala Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 225 230 235 240 Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> 35 <211> 105 <212> PRT <213> Artificial Sequence <220> <223> human kappa constant light chain amino acid sequence <400> 35 Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 1 5 10 15 Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 20 25 30 Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 35 40 45 Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 50 55 60 Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 65 70 75 80 Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 85 90 95 Thr Lys Ser Phe Asn Arg Gly Glu Cys 100 105 <210> 36 <211> 265 <212> PRT <213> Artificial Sequence <220> <223> anti-VEGF ScFv amino acid sequence <400> 36 Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 1 5 10 15 Val His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 20 25 30 Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asn Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 50 55 60 Glu Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala 65 70 75 80 Ala Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser 85 90 95 Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 100 105 110 Tyr Tyr Cys Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr 115 120 125 Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly 130 135 140 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln 145 150 155 160 Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 165 170 175 Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn Trp 180 185 190 Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile Tyr Phe Thr 195 200 205 Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 210 215 220 Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 225 230 235 240 Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp Thr Phe Gly 245 250 255 Gln Gly Thr Lys Val Glu Ile Lys Arg 260 265 <210> 37 <211> 260 <212> PRT <213> Artificial Sequence <220> <223> anti-PD-L1 ScFv amino acid sequence <400> 37 Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 1 5 10 15 Val His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 20 25 30 Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 35 40 45 Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 50 55 60 Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 65 70 75 80 Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 85 90 95 Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 100 105 110 Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 115 120 125 Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly 130 135 140 Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro 145 150 155 160 Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 165 170 175 Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro 180 185 190 Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser 195 200 205 Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 210 215 220 Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 225 230 235 240 Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val 245 250 255 Glu Ile Lys Arg 260 <210> 38 <211> 239 <212> PRT <213> Artificial Sequence <220> <223> Green fluorescent protein amino acid sequence <400> 38 Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 1 5 10 15 Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 20 25 30 Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 35 40 45 Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 50 55 60 Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 65 70 75 80 Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 85 90 95 Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 100 105 110 Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 115 120 125 Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 130 135 140 Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 145 150 155 160 Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 165 170 175 Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 180 185 190 Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 195 200 205 Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 210 215 220 Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 225 230 235 <210> 39 <211> 550 <212> PRT <213> Artificial Sequence <220> <223> Luciferase amino acid sequence <400> 39 Met Glu Asp Ala Lys Asn Ile Lys Lys Gly Pro Ala Pro Phe Tyr Pro 1 5 10 15 Leu Glu Asp Gly Thr Ala Gly Glu Gln Leu His Lys Ala Met Lys Arg 20 25 30 Tyr Ala Leu Val Pro Gly Thr Ile Ala Phe Thr Asp Ala His Ile Glu 35 40 45 Val Asp Ile Thr Tyr Ala Glu Tyr Phe Glu Met Ser Val Arg Leu Ala 50 55 60 Glu Ala Met Lys Arg Tyr Gly Leu Asn Thr Asn His Arg Ile Val Val 65 70 75 80 Cys Ser Glu Asn Ser Leu Gln Phe Phe Met Pro Val Leu Gly Ala Leu 85 90 95 Phe Ile Gly Val Ala Val Ala Pro Ala Asn Asp Ile Tyr Asn Glu Arg 100 105 110 Glu Leu Leu Asn Ser Met Gly Ile Ser Gln Pro Thr Val Val Phe Val 115 120 125 Ser Lys Lys Gly Leu Gln Lys Ile Leu Asn Val Gln Lys Lys Leu Pro 130 135 140 Ile Ile Gln Lys Ile Ile Ile Met Asp Ser Lys Thr Asp Tyr Gln Gly 145 150 155 160 Phe Gln Ser Met Tyr Thr Phe Val Thr Ser His Leu Pro Pro Gly Phe 165 170 175 Asn Glu Tyr Asp Phe Val Pro Glu Ser Phe Asp Arg Asp Lys Thr Ile 180 185 190 Ala Leu Ile Met Asn Ser Ser Gly Ser Thr Gly Leu Pro Lys Gly Val 195 200 205 Ala Leu Pro His Arg Thr Ala Cys Val Arg Phe Ser His Ala Arg Asp 210 215 220 Pro Ile Phe Gly Asn Gln Ile Ile Pro Asp Thr Ala Ile Leu Ser Val 225 230 235 240 Val Pro Phe His His Gly Phe Gly Met Phe Thr Thr Leu Gly Tyr Leu 245 250 255 Ile Cys Gly Phe Arg Val Val Leu Met Tyr Arg Phe Glu Glu Glu Leu 260 265 270 Phe Leu Arg Ser Leu Gln Asp Tyr Lys Ile Gln Ser Ala Leu Leu Val 275 280 285 Pro Thr Leu Phe Ser Phe Phe Ala Lys Ser Thr Leu Ile Asp Lys Tyr 290 295 300 Asp Leu Ser Asn Leu His Glu Ile Ala Ser Gly Gly Ala Pro Leu Ser 305 310 315 320 Lys Glu Val Gly Glu Ala Val Ala Lys Arg Phe His Leu Pro Gly Ile 325 330 335 Arg Gln Gly Tyr Gly Leu Thr Glu Thr Thr Ser Ala Ile Leu Ile Thr 340 345 350 Pro Glu Gly Asp Asp Lys Pro Gly Ala Val Gly Lys Val Val Pro Phe 355 360 365 Phe Glu Ala Lys Val Val Asp Leu Asp Thr Gly Lys Thr Leu Gly Val 370 375 380 Asn Gln Arg Gly Glu Leu Cys Val Arg Gly Pro Met Ile Met Ser Gly 385 390 395 400 Tyr Val Asn Asn Pro Glu Ala Thr Asn Ala Leu Ile Asp Lys Asp Gly 405 410 415 Trp Leu His Ser Gly Asp Ile Ala Tyr Trp Asp Glu Asp Glu His Phe 420 425 430 Phe Ile Val Asp Arg Leu Lys Ser Leu Ile Lys Tyr Lys Gly Tyr Gln 435 440 445 Val Ala Pro Ala Glu Leu Glu Ser Ile Leu Leu Gln His Pro Asn Ile 450 455 460 Phe Asp Ala Gly Val Ala Gly Leu Pro Asp Asp Asp Ala Gly Glu Leu 465 470 475 480 Pro Ala Ala Val Val Val Leu Glu His Gly Lys Thr Met Thr Glu Lys 485 490 495 Glu Ile Val Asp Tyr Val Ala Ser Gln Val Thr Thr Ala Lys Lys Leu 500 505 510 Arg Gly Gly Val Val Phe Val Asp Glu Val Pro Lys Gly Leu Thr Gly 515 520 525 Lys Leu Asp Ala Arg Lys Ile Arg Glu Ile Leu Ile Lys Ala Lys Lys 530 535 540 Gly Gly Lys Ile Ala Val 545 550 <210> 40 <211> 233 <212> PRT <213> Artificial Sequence <220> <223> Human Tumour necrosis factor alpha amino acid sequence <400> 40 Met Ser Thr Glu Ser Met Ile Arg Asp Val Glu Leu Ala Glu Glu Ala 1 5 10 15 Leu Pro Lys Lys Thr Gly Gly Pro Gln Gly Ser Arg Arg Cys Leu Phe 20 25 30 Leu Ser Leu Phe Ser Phe Leu Ile Val Ala Gly Ala Thr Thr Leu Phe 35 40 45 Cys Leu Leu His Phe Gly Val Ile Gly Pro Gln Arg Glu Glu Phe Pro 50 55 60 Arg Asp Leu Ser Leu Ile Ser Pro Leu Ala Gln Ala Val Arg Ser Ser 65 70 75 80 Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val Val Ala Asn Pro 85 90 95 Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg Ala Asn Ala Leu 100 105 110 Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu Val Val Pro Ser 115 120 125 Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe Lys Gly Gln Gly 130 135 140 Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile Ser Arg Ile Ala 145 150 155 160 Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala Ile Lys Ser Pro 165 170 175 Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu 180 185 190 Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys Gly Asp Arg Leu 195 200 205 Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly 210 215 220 Gln Val Tyr Phe Gly Ile Ile Ala Leu 225 230 <210> 41 <211> 166 <212> PRT <213> Artificial Sequence <220> <223> Human Interferon gamma amino acid sequence <400> 41 Met Lys Tyr Thr Ser Tyr Ile Leu Ala Phe Gln Leu Cys Ile Val Leu 1 5 10 15 Gly Ser Leu Gly Cys Tyr Cys Gln Asp Pro Tyr Val Lys Glu Ala Glu 20 25 30 Asn Leu Lys Lys Tyr Phe Asn Ala Gly His Ser Asp Val Ala Asp Asn 35 40 45 Gly Thr Leu Phe Leu Gly Ile Leu Lys Asn Trp Lys Glu Glu Ser Asp 50 55 60 Arg Lys Ile Met Gln Ser Gln Ile Val Ser Phe Tyr Phe Lys Leu Phe 65 70 75 80 Lys Asn Phe Lys Asp Asp Gln Ser Ile Gln Lys Ser Val Glu Thr Ile 85 90 95 Lys Glu Asp Met Asn Val Lys Phe Phe Asn Ser Asn Lys Lys Lys Arg 100 105 110 Asp Asp Phe Glu Lys Leu Thr Asn Tyr Ser Val Thr Asp Leu Asn Val 115 120 125 Gln Arg Lys Ala Ile His Glu Leu Ile Gln Val Met Ala Glu Leu Ser 130 135 140 Pro Ala Ala Lys Thr Gly Lys Arg Lys Arg Ser Gln Met Leu Phe Arg 145 150 155 160 Gly Arg Arg Ala Ser Gln 165 <210> 42 <211> 188 <212> PRT <213> Artificial Sequence <220> <223> Human Interferon alpha amino acid sequence <400> 42 Met Ala Leu Thr Phe Ala Leu Leu Val Ala Leu Leu Val Leu Ser Cys 1 5 10 15 Lys Ser Ser Cys Ser Val Gly Cys Asp Leu Pro Gln Thr His Ser Leu 20 25 30 Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile Ser 35 40 45 Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu 50 55 60 Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His 65 70 75 80 Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser Ser 85 90 95 Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr 100 105 110 Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val 115 120 125 Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys 130 135 140 Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro 145 150 155 160 Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu 165 170 175 Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys Glu 180 185 <210> 43 <211> 180 <212> PRT <213> Artificial Sequence <220> <223> human cancer/testis angtigen 1 (NY-ESO-1) amino acid sequence <400> 43 Met Gln Ala Glu Gly Arg Gly Thr Gly Gly Ser Thr Gly Asp Ala Asp 1 5 10 15 Gly Pro Gly Gly Pro Gly Ile Pro Asp Gly Pro Gly Gly Asn Ala Gly 20 25 30 Gly Pro Gly Glu Ala Gly Ala Thr Gly Gly Arg Gly Pro Arg Gly Ala 35 40 45 Gly Ala Ala Arg Ala Ser Gly Pro Gly Gly Gly Ala Pro Arg Gly Pro 50 55 60 His Gly Gly Ala Ala Ser Gly Leu Asn Gly Cys Cys Arg Cys Gly Ala 65 70 75 80 Arg Gly Pro Glu Ser Arg Leu Leu Glu Phe Tyr Leu Ala Met Pro Phe 85 90 95 Ala Thr Pro Met Glu Ala Glu Leu Ala Arg Arg Ser Leu Ala Gln Asp 100 105 110 Ala Pro Pro Leu Pro Val Pro Gly Val Leu Leu Lys Glu Phe Thr Val 115 120 125 Ser Gly Asn Ile Leu Thr Ile Arg Leu Thr Ala Ala Asp His Arg Gln 130 135 140 Leu Gln Leu Ser Ile Ser Ser Cys Leu Gln Gln Leu Ser Leu Leu Met 145 150 155 160 Trp Ile Thr Gln Cys Phe Leu Pro Val Phe Leu Ala Gln Pro Pro Ser 165 170 175 Gly Gln Arg Arg 180 <210> 44 <211> 1255 <212> PRT <213> Artificial Sequence <220> <223> human MUC-1 amino acid sequence <400> 44 Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 1 5 10 15 Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 20 25 30 Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 35 40 45 Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 50 55 60 Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 65 70 75 80 Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Thr Trp Gly Gln 85 90 95 Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Ser Thr Thr 100 105 110 Pro Pro Ala His Asp Val Thr Ser Ala Pro Asp Asn Lys Pro Ala Pro 115 120 125 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 130 135 140 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 145 150 155 160 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 165 170 175 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 180 185 190 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 195 200 205 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 210 215 220 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 225 230 235 240 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 245 250 255 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 260 265 270 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 275 280 285 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 290 295 300 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 305 310 315 320 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 325 330 335 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 340 345 350 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 355 360 365 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 370 375 380 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 385 390 395 400 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 405 410 415 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 420 425 430 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 435 440 445 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 450 455 460 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 465 470 475 480 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 485 490 495 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 500 505 510 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 515 520 525 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 530 535 540 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 545 550 555 560 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 565 570 575 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 580 585 590 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 595 600 605 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 610 615 620 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 625 630 635 640 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 645 650 655 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 660 665 670 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 675 680 685 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 690 695 700 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 705 710 715 720 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 725 730 735 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 740 745 750 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 755 760 765 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 770 775 780 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 785 790 795 800 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 805 810 815 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 820 825 830 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 835 840 845 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 850 855 860 Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 865 870 875 880 Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 885 890 895 Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 900 905 910 Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 915 920 925 Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn 930 935 940 Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Val His Asn Val Thr Ser 945 950 955 960 Ala Ser Gly Ser Ala Ser Gly Ser Ala Ser Thr Leu Val His Asn Gly 965 970 975 Thr Ser Ala Arg Ala Thr Thr Thr Pro Ala Ser Lys Ser Thr Pro Phe 980 985 990 Ser Ile Pro Ser His His Ser Asp Thr Pro Thr Thr Leu Ala Ser His 995 1000 1005 Ser Thr Lys Thr Asp Ala Ser Ser Thr His His Ser Ser Val Pro 1010 1015 1020 Pro Leu Thr Ser Ser Asn His Ser Thr Ser Pro Gln Leu Ser Thr 1025 1030 1035 Gly Val Ser Phe Phe Phe Leu Ser Phe His Ile Ser Asn Leu Gln 1040 1045 1050 Phe Asn Ser Ser Leu Glu Asp Pro Ser Thr Asp Tyr Tyr Gln Glu 1055 1060 1065 Leu Gln Arg Asp Ile Ser Glu Met Phe Leu Gln Ile Tyr Lys Gln 1070 1075 1080 Gly Gly Phe Leu Gly Leu Ser Asn Ile Lys Phe Arg Pro Gly Ser 1085 1090 1095 Val Val Val Gln Leu Thr Leu Ala Phe Arg Glu Gly Thr Ile Asn 1100 1105 1110 Val His Asp Val Glu Thr Gln Phe Asn Gln Tyr Lys Thr Glu Ala 1115 1120 1125 Ala Ser Arg Tyr Asn Leu Thr Ile Ser Asp Val Ser Val Ser Asp 1130 1135 1140 Val Pro Phe Pro Phe Ser Ala Gln Ser Gly Ala Gly Val Pro Gly 1145 1150 1155 Trp Gly Ile Ala Leu Leu Val Leu Val Cys Val Leu Val Ala Leu 1160 1165 1170 Ala Ile Val Tyr Leu Ile Ala Leu Ala Val Cys Gln Cys Arg Arg 1175 1180 1185 Lys Asn Tyr Gly Gln Leu Asp Ile Phe Pro Ala Arg Asp Thr Tyr 1190 1195 1200 His Pro Met Ser Glu Tyr Pro Thr Tyr His Thr His Gly Arg Tyr 1205 1210 1215 Val Pro Pro Ser Ser Thr Asp Arg Ser Pro Tyr Glu Lys Val Ser 1220 1225 1230 Ala Gly Asn Gly Gly Ser Ser Leu Ser Tyr Thr Asn Pro Ala Val 1235 1240 1245 Ala Ala Thr Ser Ala Asn Leu 1250 1255 <210> 45 <211> 9 <212> DNA <213> Artificial Sequence <220> <223> A Kozak sequence <400> 45 000 9 <210> 46 <211> 35334 <212> DNA <213> Artificial Sequence <220> <223> NG-177 virus genome sequence consisting of a transgene cassette encoding an anti-PD-L1 full length antibody inserted into the region BY. <400> 46 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac ctttaaaggt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgca ttatcctgtt 29400 tctggttgct actgccactg gagtgcatag cgaggtacag cttgtcgagt ctggaggtgg 29460 gctggtacaa ccaggcggat ccctcagact gtcttgtgcc gcatctggct tcacgttcag 29520 cgatagttgg atacactggg tgaggcaggc accagggaaa ggtctggagt gggtggcttg 29580 gataagccca tatggtggct ccacatatta cgccgactca gtgaagggtc gcttcaccat 29640 ttctgctgat acgtcaaaga acacagccta tctccagatg aactcccttc gtgccgagga 29700 taccgctgtc tactattgtg cccggagaca ttggcctggc gggttcgact actggggaca 29760 ggggactctc gtcactgttt ctgcagcgtc tactaaaggg cccagcgtgt ttcctcttgc 29820 tccctccagt aagtcaacta gtggcggcac cgctgctttg ggctgcctgg tcaaggacta 29880 ttttccagag cctgtaaccg tgagctggaa tagtggagca ttgacctcag gagtgcacac 29940 atttcccgca gtgctgcagt caagcggact gtacagcctt tccagtgtgg tgaccgttcc 30000 ttcctcaagt ctgggcactc aaacctacat ctgcaacgtg aaccacaaac ccagcaatac 30060 caaagtcgac aagaaggttg agccgaagag ctgtgacaaa acgcacacat gccctccatg 30120 tcccgcccca gaactgctcg gtggaccgtc cgtgttcctg tttcctccga agcccaagga 30180 cacactgatg atcagtcgca ccccagaagt cacctgtgtg gtggtggcgg taagccacga 30240 agatcccgag gtcaagttca attggtatgt cgatggggtt gaggtccaca atgccaagac 30300 taagccaagg gaggaacagt atgccagcac ataccgagtc gtgtctgtgc tgaccgttct 30360 gcatcaggac tggctcaacg ggaaagagta caagtgcaaa gtgtccaaca aggcacttcc 30420 tgcccctatc gagaaaacca tcagcaaggc gaaaggtcag cccagagaac cacaagtcta 30480 cacactgcca ccctctcggg acgaactcac aaagaatcag gtgtcactga catgcttggt 30540 taaaggcttt taccccagcg atattgccgt ggaatgggaa tccaatgggc aacctgagaa 30600 caactacaag actacacctc ccgtattgga tagcgatgga agcttcttcc tgtactctaa 30660 gttgacggtt gacaaatcca ggtggcagca gggcaatgtg ttttcttgct ccgtaatgca 30720 tgaagctctg cataatcact atacccagaa atccctctca ctgagtccgg gcaaatgact 30780 cgaggtttct agaagttgtc tcctcctgca ctgactgact gacctaacgt tactggccga 30840 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 30900 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 30960 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 31020 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 31080 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 31140 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 31200 ctcccctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 31260 ggatctgatc tggggcctcg gtgcacatgc ttttcatgtg tttagtcgag gttaaaaaac 31320 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataataac 31380 atggactgga cctggcgcgt gttttgcctg ctcgccgtgg ctcctggggc ccacagcgat 31440 atccaaatga ctcagtctcc ctcttcactg tctgcaagcg ttggcgatag ggtgacaatt 31500 acctgtagag cgagtcagga cgtttcaacc gctgtagcct ggtaccagca gaaaccggga 31560 aaagccccta agctgctcat ttactccgct tcctttctct attccggagt gcctagccga 31620 tttagtggct ctggttcagg gactgacttc acactgacca tatccagtct gcaaccagag 31680 gatttcgcga cgtactactg tcagcagtat ctgtatcacc ccgctacatt tgggcaaggg 31740 acaaaagtgg agatcaaacg gactgtagcc gcacccagcg tgttcatctt tccaccctct 31800 gatgagcagc ttaagagcgg tacggcatct gtcgtgtgct tgctgaacaa cttctatcct 31860 cgtgaagcca aagtccagtg gaaggtggac aatgccttgc agtcaggcaa ctcacaggaa 31920 agcgtcactg aacaggacag caaggatagt acctacagcc tgtccagcac actgactctc 31980 tccaaagccg actatgagaa gcacaaggtt tacgcttgcg aagtgaccca tcaaggcctt 32040 agcagtccag tcaccaagtc cttcaatcgc ggagagtgct gagctagctt gactgactga 32100 gatacagcgt accttcagct cacagacatg ataagataca ttgatgagtt tggacaaacc 32160 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 32220 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 32280 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 32340 ggtagtcgtc agctatcctg caggaacttg tttatttgaa aatcaattca caaaatccga 32400 gtagttattt tgcctccccc ttcccattta acagaataca ccaatctctc cccacgcaca 32460 gctttaaaca tttggatacc attagatata gacatggttt tagattccac attccaaaca 32520 gtttcagagc gagccaatct ggggtcagtg atagataaaa atccatcggg atagtctttt 32580 aaagcgcttt cacagtccaa ctgctgcgga tgcgactccg gagtctggat cacggtcatc 32640 tggaagaaga acgatgggaa tcataatccg aaaacggtat cggacgattg tgtctcatca 32700 aacccacaag cagccgctgt ctgcgtcgct ccgtgcgact gctgtttatg ggatcagggt 32760 ccacagtgtc ctgaagcatg attttaatag cccttaacat caactttctg gtgcgatgcg 32820 cgcagcaacg cattctgatt tcactcaaat ctttgcagta ggtacaacac attattacaa 32880 tattgtttaa taaaccataa ttaaaagcgc tccagccaaa actcatatct gatataatcg 32940 cccctgcatg accatcatac caaagtttaa tataaattaa atgacgttcc ctcaaaaaca 33000 cactacccac atacatgatc tcttttggca tgtgcatatt aacaatctgt ctgtaccatg 33060 gacaacgttg gttaatcatg caacccaata taaccttccg gaaccacact gccaacaccg 33120 ctcccccagc catgcattga agtgaaccct gctgattaca atgacaatga agaacccaat 33180 tctctcgacc gtgaatcact tgagaatgaa aaatatctat agtggcacaa catagacata 33240 aatgcatgca tcttctcata atttttaact cctcaggatt tagaaacata tcccagggaa 33300 taggaagctc ttgcagaaca gtaaagctgg cagaacaagg aagaccacga acacaactta 33360 cactatgcat agtcatagta tcacaatctg gcaacagcgg gtggtcttca gtcatagaag 33420 ctcgggtttc attttcctca caacgtggta actgggctct ggtgtaaggg tgatgtctgg 33480 cgcatgatgt cgagcgtgcg cgcaaccttg tcataatgga gttgcttcct gacattctcg 33540 tattttgtat agcaaaacgc ggccctggca gaacacactc ttcttcgcct tctatcctgc 33600 cgcttagcgt gttccgtgtg atagttcaag tacaaccaca ctcttaagtt ggtcaaaaga 33660 atgctggctt cagttgtaat caaaactcca tcgcatctaa tcgttctgag gaaatcatcc 33720 aagcaatgca actggattgt gtttcaagca ggagaggaga gggaagagac ggaagaacca 33780 tgttaatttt tattccaaac gatctcgcag tacttcaaat tgtagatcgc gcagatggca 33840 tctctcgccc ccactgtgtt ggtgaaaaag cacagctaga tcaaaagaaa tgcgattttc 33900 aaggtgctca acggtggctt ccagcaaagc ctccacgcgc acatccaaga acaaaagaat 33960 accaaaagaa ggagcatttt ctaactcctc aatcatcata ttacattcct gcaccattcc 34020 cagataattt tcagctttcc agccttgaat tattcgtgtc agttcttgtg gtaaatccaa 34080 tccacacatt acaaacaggt cccggagggc gccctccacc accattctta aacacaccct 34140 cataatgaca aaatatcttg ctcctgtgtc acctgtagcg aattgagaat ggcaacatca 34200 attgacatgc ccttggctct aagttcttct ttaagttcta gttgtaaaaa ctctctcata 34260 ttatcaccaa actgcttagc cagaagcccc ccgggaacaa gagcagggga cgctacagtg 34320 cagtacaagc gcagacctcc ccaattggct ccagcaaaaa caagattgga ataagcatat 34380 tgggaaccgc cagtaatatc atcgaagttg ctggaaatat aatcaggcag agtttcttgt 34440 aaaaattgaa taaaagaaaa atttgccaaa aaaacattca aaacctctgg gatgcaaatg 34500 caataggtta ccgcgctgcg ctccaacatt gttagttttg aattagtctg caaaaataaa 34560 aaaaaaaaca agcgtcatat catagtagcc tgacgaacag atggataaat cagtctttcc 34620 atcacaagac aagccacagg gtctccagct cgaccctcgt aaaacctgtc atcatgatta 34680 aacaacagca ccgaaagttc ctcgcggtga ccagcatgaa taattcttga tgaagcatac 34740 aatccagaca tgttagcatc agttaacgag aaaaaacagc caacatagcc tttgggtata 34800 attatgctta atcgtaagta tagcaaagcc acccctcgcg gatacaaagt aaaaggcaca 34860 ggagaataaa aaatataatt atttctctgc tgctgttcag gcaacgtcgc ccccggtccc 34920 tctaaataca catacaaagc ctcatcagcc atggcttacc agacaaagta cagcgggcac 34980 acaaagcaca agctctaaag tgactctcca acctctccac aatatatata tacacaagcc 35040 ctaaactgac gtaatgggag taaagtgtaa aaaatcccgc caaacccaac acacaccccg 35100 aaactgcgtc accagggaaa agtacagttt cacttccgca atcccaacag gcgtaacttc 35160 ctctttctca cggtacgtga tatcccacta acttgcaacg tcattttccc acggtcgcac 35220 cgcccctttt agccgttaac cccacagcca atcaccacac gatccacact ttttaaaatc 35280 acctcattta catattggca ccattccatc tataaggtat attatataga taga 35334 <210> 47 <211> 5288 <212> DNA <213> Artificial Sequence <220> <223> DNA sequence corresponding to E2B region of the EnAd genome (bp 10355-5068) <400> 47 ctatggcatc tcgatccagc agacctcctc gtttcgcggg tttggacggc tcctggaata 60 gggtatgaga cgatgggcgt ccagcgctgc cagggttcgg tccttccagg gtctcagtgt 120 tcgagtcagg gttgtttccg tcacagtgaa ggggtgtgcg cctgcttggg cgcttgccag 180 ggtgcgcttc agactcatcc tgctggtcga aaacttctgt cgcttggcgc cctgtatgtc 240 ggccaagtag cagtttacca tgagttcgta gttgagcgcc tcggctgcgt ggcctttggc 300 gcggagctta cctttggaag ttttcttgca taccgggcag tataggcatt tcagcgcata 360 caacttgggc gcaaggaaaa cggattctgg ggagtatgca tctgcgccgc aggaggcgca 420 aacagtttca cattccacca gccaggttaa atccggttca ttggggtcaa aaacaagttt 480 tccgccatat tttttgatgc gtttcttacc tttggtctcc atgagttcgt gtcctcgttg 540 agtgacaaac aggctgtccg tgtccccgta gactgatttt acaggcctct tctccagtgg 600 agtgcctcgg tcttcttcgt acaggaactc tgaccactct gatacaaagg cgcgcgtcca 660 ggccagcaca aaggaggcta tgtgggaggg gtagcgatcg ttgtcaacca gggggtccac 720 cttttccaaa gtatgcaaac acatgtcacc ctcttcaaca tccaggaatg tgattggctt 780 gtaggtgtat ttcacgtgac ctggggtccc cgctgggggg gtataaaagg gggcggttct 840 ttgctcttcc tcactgtctt ccggatcgct gtccaggaac gtcagctgtt ggggtaggta 900 ttccctctcg aaggcgggca tgacctctgc actcaggttg tcagtttcta agaacgagga 960 ggatttgata ttgacagtgc cggttgagat gcctttcatg aggttttcgt ccatctggtc 1020 agaaaacaca atttttttat tgtcaagttt ggtggcaaat gatccataca gggcgttgga 1080 taaaagtttg gcaatggatc gcatggtttg gttcttttcc ttgtccgcgc gctctttggc 1140 ggcgatgttg agttggacat actcgcgtgc caggcacttc cattcgggga agatagttgt 1200 taattcatct ggcacgattc tcacttgcca ccctcgatta tgcaaggtaa ttaaatccac 1260 actggtggcc acctcgcctc gaaggggttc attggtccaa cagagcctac ctcctttcct 1320 agaacagaaa gggggaagtg ggtctagcat aagttcatcg ggagggtctg catccatggt 1380 aaagattccc ggaagtaaat ccttatcaaa atagctgatg ggagtggggt catctaaggc 1440 catttgccat tctcgagctg ccagtgcgcg ctcatatggg ttaaggggac tgccccatgg 1500 catgggatgg gtgagtgcag aggcatacat gccacagatg tcatagacgt agatgggatc 1560 ctcaaagatg cctatgtagg ttggatagca tcgcccccct ctgatacttg ctcgcacata 1620 gtcatatagt tcatgtgatg gcgctagcag ccccggaccc aagttggtgc gattgggttt 1680 ttctgttctg tagacgatct ggcgaaagat ggcgtgagaa ttggaagaga tggtgggtct 1740 ttgaaaaatg ttgaaatggg catgaggtag acctacagag tctctgacaa agtgggcata 1800 agattcttga agcttggtta ccagttcggc ggtgacaagt acgtctaggg cgcagtagtc 1860 aagtgtttct tgaatgatgt cataacctgg ttggtttttc ttttcccaca gttcgcggtt 1920 gagaaggtat tcttcgcgat ccttccagta ctcttctagc ggaaacccgt ctttgtctgc 1980 acggtaagat cctagcatgt agaactgatt aactgccttg taagggcagc agcccttctc 2040 tacgggtaga gagtatgctt gagcagcttt tcgtagcgaa gcgtgagtaa gggcaaaggt 2100 gtctctgacc atgactttga ggaattggta tttgaagtcg atgtcgtcac aggctccctg 2160 ttcccagagt tggaagtcta cccgtttctt gtaggcgggg ttgggcaaag cgaaagtaac 2220 atcattgaag agaatcttgc cggccctggg catgaaattg cgagtgatgc gaaaaggctg 2280 tggtacttcc gctcggttat tgataacctg ggcagctagg acgatctcgt cgaaaccgtt 2340 gatgttgtgt cctacgatgt ataattctat gaaacgcggc gtgcctctga cgtgaggtag 2400 cttactgagc tcatcaaagg ttaggtctgt ggggtcagat aaggcgtagt gttcgagagc 2460 ccattcgtgc aggtgaggat tcgctttaag gaaggaggac cagaggtcca ctgccagtgc 2520 tgtttgtaac tggtcccggt actgacgaaa atgccgtccg actgccattt tttctggggt 2580 gacgcaatag aaggtttggg ggtcctgccg ccagcgatcc cacttgagtt ttatggcgag 2640 gtcataggcg atgttgacga gccgctggtc tccagagagt ttcatgacca gcatgaaggg 2700 gattagctgc ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgagaaa 2760 gagcctttct gtgcgaggat gagagccaat cgggaagaac tggatctcct gccaccagtt 2820 ggaggaatgg ctgttgatgt gatggaagta gaactccctg cgacgcgccg agcattcatg 2880 cttgtgcttg tacagacggc cgcagtagtc gcagcgttgc acgggttgta tctcgtgaat 2940 gagttgtacc tggcttccct tgacgagaaa tttcagtggg aagccgaggc ctggcgattg 3000 tatctcgtgc tttactatgt tgtctgcatc ggcctgttca tcttctgtct cgatggtggt 3060 catgctgacg agccctcgcg ggaggcaagt ccagacctcg gcgcggcagg ggcggagctc 3120 gaggacgaga gcgcgcaggc tggagctgtc cagggtcctg agacgctgcg gactcaggtt 3180 agtaggcagt gtcaggagat taacttgcat gatcttttgg agggcgtgcg ggaggttcag 3240 atagtacttg atctcaacgg gtccgttggt ggagatgtcg atggcttgca gggttccgtg 3300 tcccttgggc gctaccaccg tgcccttgtt tttcattttg gacggcggtg gctctgttgc 3360 ttcttgcatg tttagaagcg gtgtcgaggg cgcgcaccgg gcggcagggg cggctcggga 3420 cccggcggca tggctggcag tggtacgtcg gcgccgcgcg cgggtaggtt ctggtactgc 3480 gccctgagaa gactcgcatg cgcgacgacg cggcggttga catcctggat ctgacgcctc 3540 tgggtgaaag ctaccggccc cgtgagcttg aacctgaaag agagttcaac agaatcaatc 3600 tcggtatcgt tgacggcggc ttgcctaagg atttcttgca cgtcaccaga gttgtcctgg 3660 taggcgatct ccgccatgaa ctgctcgatc tcttcctctt gaagatctcc gcggcccgct 3720 ctctcgacgg tggccgcgag gtcgttggag atgcgcccaa tgagttgaga gaatgcattc 3780 atgcccgcct cgttccagac gcggctgtag accacggccc ccacgggatc tctcgcgcgc 3840 atgaccacct gggcgaggtt gagctccacg tggcgggtga agaccgcata gttgcatagg 3900 cgctggaaaa ggtagttgag tgtggtggcg atgtgctcgg tgacgaagaa atacatgatc 3960 catcgtctca gcggcatctc gctgacatcg cccagagctt ccaagcgctc catggcctcg 4020 tagaagtcca cggcaaaatt aaaaaactgg gagtttcgcg cggacacggt caactcctct 4080 tccagaagac ggataagttc ggcgatggtg gtgcgcacct cgcgctcgaa agcccctggg 4140 atttcttcct caatctcttc ttcttccact aacatctctt cctcttcagg tggggctgca 4200 ggaggagggg gaacgcggcg acgccggcgg cgcacgggca gacggtcgat gaatctttca 4260 atgacctctc cgcggcggcg gcgcatggtt tcagtgacgg cgcggccgtt ctcgcgcggt 4320 cgcagagtaa aaacaccgcc gcgcatctcc ttaaagtggt gactgggagg ttctccgttt 4380 gggagggaga gggcgctgat tatacatttt attaattggc ccgtagggac tgcacgcaga 4440 gatctgatcg tgtcaagatc cacgggatct gaaaaccttt cgacgaaagc gtctaaccag 4500 tcacagtcac aaggtaggct gagtacggct tcttgtgggc gggggtggtt atgtgttcgg 4560 tctgggtctt ctgtttcttc ttcatctcgg gaaggtgaga cgatgctgct ggtgatgaaa 4620 ttaaagtagg cagttctaag acggcggatg gtggcgagga gcaccaggtc tttgggtccg 4680 gcttgctgga tacgcaggcg attggccatt ccccaagcat tatcctgaca tctagcaaga 4740 tctttgtagt agtcttgcat gagccgttct acgggcactt cttcctcacc cgttctgcca 4800 tgcatacgtg tgagtccaaa tccgcgcatt ggttgtacca gtgccaagtc agctacgact 4860 ctttcggcga ggatggcttg ctgtacttgg gtaagggtgg cttgaaagtc atcaaaatcc 4920 acaaagcggt ggtaagctcc tgtattaatg gtgtaagcac agttggccat gactgaccag 4980 ttaactgtct ggtgaccagg gcgcacgagc tcggtgtatt taaggcgcga ataggcgcgg 5040 gtgtcaaaga tgtaatcgtt gcaggtgcgc accagatact ggtaccctat aagaaaatgc 5100 ggcggtggtt ggcggtagag aggccatcgt tctgtagctg gagcgccagg ggcgaggtct 5160 tccaacataa ggcggtgata gccgtagatg tacctggaca tccaggtgat tcctgcggcg 5220 gtagtagaag cccgaggaaa ctcgcgtacg cggttccaaa tgttgcgtag cggcatgaag 5280 tagttcat 5288 <210> 48 <211> 33432 <212> DNA <213> Artificial Sequence <220> <223> NG-167 virus genome sequence consisting of a transgene cassette that encodes an anti-VEGF ScFv with a C-terminal His6 tag, inserted in the region BY. <400> 48 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc accatgggat ggagctgtat catcctcttc 29400 ttggtagcaa cagctacagg tgtccactcc gaagtgcaac tggtggagtc tggcggaggt 29460 ctggtgcagc ctggaggctc tctcagactg tcatgcgctg caagcggttt taccatctct 29520 gactactgga tccattgggt gcgtcaggct cctggaaaag gccttgagtg ggttgcgggt 29580 ataactccag caggcgggta cacctactac gccgatagcg ttaaaggccg ctttaccatt 29640 agcgcggaca ccagcaagaa taccgcctat ctgcagatga actcactgag agcggaggat 29700 acagcagtgt actattgcgc aagattcgtg ttctttctgc catacgctat ggactattgg 29760 ggccaaggga cactggttac agtatcctcg ggtggcggtg gctcgggcgg tggtggatct 29820 ggtggcggcg gatctgacat ccagatgacc cagagcccat caagtctgtc cgccagcgtt 29880 ggcgatcgag tcaccattac ttgtagggct tctcaggacg tgtctacagc tgttgcttgg 29940 tatcagcaga aaccagggaa agcacccaaa cttctgatct actcagcttc cttcctgtac 30000 tcaggagttc ctagtcggtt tagtggcagc ggcagtggca cagacttcac gctcaccatt 30060 agcagtctcc agcctgagga ttttgccact tactactgtc agcaagggta tggaaatccc 30120 ttcacattcg ggcaaggaac caaagtcgag attaagaggc atcatcacca ccaccattaa 30180 gctagcttga ctgactgaga tacagcgtac cttcagctca cagacatgat aagatacatt 30240 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 30300 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 30360 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 30420 taaaacctct acaaatgtgg tagtcgtcag ctatcctgca ggaacttgtt tatttgaaaa 30480 tcaattcaca aaatccgagt agttattttg cctccccctt cccatttaac agaatacacc 30540 aatctctccc cacgcacagc tttaaacatt tggataccat tagatataga catggtttta 30600 gattccacat tccaaacagt ttcagagcga gccaatctgg ggtcagtgat agataaaaat 30660 ccatcgggat agtcttttaa agcgctttca cagtccaact gctgcggatg cgactccgga 30720 gtctggatca cggtcatctg gaagaagaac gatgggaatc ataatccgaa aacggtatcg 30780 gacgattgtg tctcatcaaa cccacaagca gccgctgtct gcgtcgctcc gtgcgactgc 30840 tgtttatggg atcagggtcc acagtgtcct gaagcatgat tttaatagcc cttaacatca 30900 actttctggt gcgatgcgcg cagcaacgca ttctgatttc actcaaatct ttgcagtagg 30960 tacaacacat tattacaata ttgtttaata aaccataatt aaaagcgctc cagccaaaac 31020 tcatatctga tataatcgcc cctgcatgac catcatacca aagtttaata taaattaaat 31080 gacgttccct caaaaacaca ctacccacat acatgatctc ttttggcatg tgcatattaa 31140 caatctgtct gtaccatgga caacgttggt taatcatgca acccaatata accttccgga 31200 accacactgc caacaccgct cccccagcca tgcattgaag tgaaccctgc tgattacaat 31260 gacaatgaag aacccaattc tctcgaccgt gaatcacttg agaatgaaaa atatctatag 31320 tggcacaaca tagacataaa tgcatgcatc ttctcataat ttttaactcc tcaggattta 31380 gaaacatatc ccagggaata ggaagctctt gcagaacagt aaagctggca gaacaaggaa 31440 gaccacgaac acaacttaca ctatgcatag tcatagtatc acaatctggc aacagcgggt 31500 ggtcttcagt catagaagct cgggtttcat tttcctcaca acgtggtaac tgggctctgg 31560 tgtaagggtg atgtctggcg catgatgtcg agcgtgcgcg caaccttgtc ataatggagt 31620 tgcttcctga cattctcgta ttttgtatag caaaacgcgg ccctggcaga acacactctt 31680 cttcgccttc tatcctgccg cttagcgtgt tccgtgtgat agttcaagta caaccacact 31740 cttaagttgg tcaaaagaat gctggcttca gttgtaatca aaactccatc gcatctaatc 31800 gttctgagga aatcatccaa gcaatgcaac tggattgtgt ttcaagcagg agaggagagg 31860 gaagagacgg aagaaccatg ttaattttta ttccaaacga tctcgcagta cttcaaattg 31920 tagatcgcgc agatggcatc tctcgccccc actgtgttgg tgaaaaagca cagctagatc 31980 aaaagaaatg cgattttcaa ggtgctcaac ggtggcttcc agcaaagcct ccacgcgcac 32040 atccaagaac aaaagaatac caaaagaagg agcattttct aactcctcaa tcatcatatt 32100 acattcctgc accattccca gataattttc agctttccag ccttgaatta ttcgtgtcag 32160 ttcttgtggt aaatccaatc cacacattac aaacaggtcc cggagggcgc cctccaccac 32220 cattcttaaa cacaccctca taatgacaaa atatcttgct cctgtgtcac ctgtagcgaa 32280 ttgagaatgg caacatcaat tgacatgccc ttggctctaa gttcttcttt aagttctagt 32340 tgtaaaaact ctctcatatt atcaccaaac tgcttagcca gaagcccccc gggaacaaga 32400 gcaggggacg ctacagtgca gtacaagcgc agacctcccc aattggctcc agcaaaaaca 32460 agattggaat aagcatattg ggaaccgcca gtaatatcat cgaagttgct ggaaatataa 32520 tcaggcagag tttcttgtaa aaattgaata aaagaaaaat ttgccaaaaa aacattcaaa 32580 acctctggga tgcaaatgca ataggttacc gcgctgcgct ccaacattgt tagttttgaa 32640 ttagtctgca aaaataaaaa aaaaaacaag cgtcatatca tagtagcctg acgaacagat 32700 ggataaatca gtctttccat cacaagacaa gccacagggt ctccagctcg accctcgtaa 32760 aacctgtcat catgattaaa caacagcacc gaaagttcct cgcggtgacc agcatgaata 32820 attcttgatg aagcatacaa tccagacatg ttagcatcag ttaacgagaa aaaacagcca 32880 acatagcctt tgggtataat tatgcttaat cgtaagtata gcaaagccac ccctcgcgga 32940 tacaaagtaa aaggcacagg agaataaaaa atataattat ttctctgctg ctgttcaggc 33000 aacgtcgccc ccggtccctc taaatacaca tacaaagcct catcagccat ggcttaccag 33060 acaaagtaca gcgggcacac aaagcacaag ctctaaagtg actctccaac ctctccacaa 33120 tatatatata cacaagccct aaactgacgt aatgggagta aagtgtaaaa aatcccgcca 33180 aacccaacac acaccccgaa actgcgtcac cagggaaaag tacagtttca cttccgcaat 33240 cccaacaggc gtaacttcct ctttctcacg gtacgtgata tcccactaac ttgcaacgtc 33300 attttcccac ggtcgcaccg ccccttttag ccgttaaccc cacagccaat caccacacga 33360 tccacacttt ttaaaatcac ctcatttaca tattggcacc attccatcta taaggtatat 33420 tatatagata ga 33432 <210> 49 <211> 33765 <212> DNA <213> Artificial Sequence <220> <223> NG-95 virus genome sequence consisting of a transgene cassette that encodes the cytokine, IFN?, inserted in the region BY. <400> 49 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgccca ccatgaaata tacaagttat atcttggctt ttcagctctg catcgttttg 30060 ggttctcttg gctgttactg ccaggaccca tatgtaaaag aagcagaaaa ccttaagaaa 30120 tattttaatg caggtcattc agatgtagcg gataatggaa ctcttttctt aggcattttg 30180 aagaattgga aagaggagag tgacagaaaa ataatgcaga gccaaattgt ctccttttac 30240 ttcaaacttt ttaaaaactt taaagatgac cagagcatcc aaaagagtgt ggagaccatc 30300 aaggaagaca tgaatgtcaa gtttttcaat agcaacaaaa agaaacgaga tgacttcgaa 30360 aagctgacta attattcggt aactgacttg aatgtccaac gcaaagcaat acatgaactc 30420 atccaagtga tggctgaact gtcgccagca gctaaaacag ggaagcgaaa aaggagtcag 30480 atgctgtttc gaggtcgaag agcatcccag taagctagct tgactgactg agatacagcg 30540 taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac cacaactaga 30600 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 30660 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 30720 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtagtcgt 30780 cagctatcct gcaggaactt gtttatttga aaatcaattc acaaaatccg agtagttatt 30840 ttgcctcccc cttcccattt aacagaatac accaatctct ccccacgcac agctttaaac 30900 atttggatac cattagatat agacatggtt ttagattcca cattccaaac agtttcagag 30960 cgagccaatc tggggtcagt gatagataaa aatccatcgg gatagtcttt taaagcgctt 31020 tcacagtcca actgctgcgg atgcgactcc ggagtctgga tcacggtcat ctggaagaag 31080 aacgatggga atcataatcc gaaaacggta tcggacgatt gtgtctcatc aaacccacaa 31140 gcagccgctg tctgcgtcgc tccgtgcgac tgctgtttat gggatcaggg tccacagtgt 31200 cctgaagcat gattttaata gcccttaaca tcaactttct ggtgcgatgc gcgcagcaac 31260 gcattctgat ttcactcaaa tctttgcagt aggtacaaca cattattaca atattgttta 31320 ataaaccata attaaaagcg ctccagccaa aactcatatc tgatataatc gcccctgcat 31380 gaccatcata ccaaagttta atataaatta aatgacgttc cctcaaaaac acactaccca 31440 catacatgat ctcttttggc atgtgcatat taacaatctg tctgtaccat ggacaacgtt 31500 ggttaatcat gcaacccaat ataaccttcc ggaaccacac tgccaacacc gctcccccag 31560 ccatgcattg aagtgaaccc tgctgattac aatgacaatg aagaacccaa ttctctcgac 31620 cgtgaatcac ttgagaatga aaaatatcta tagtggcaca acatagacat aaatgcatgc 31680 atcttctcat aatttttaac tcctcaggat ttagaaacat atcccaggga ataggaagct 31740 cttgcagaac agtaaagctg gcagaacaag gaagaccacg aacacaactt acactatgca 31800 tagtcatagt atcacaatct ggcaacagcg ggtggtcttc agtcatagaa gctcgggttt 31860 cattttcctc acaacgtggt aactgggctc tggtgtaagg gtgatgtctg gcgcatgatg 31920 tcgagcgtgc gcgcaacctt gtcataatgg agttgcttcc tgacattctc gtattttgta 31980 tagcaaaacg cggccctggc agaacacact cttcttcgcc ttctatcctg ccgcttagcg 32040 tgttccgtgt gatagttcaa gtacaaccac actcttaagt tggtcaaaag aatgctggct 32100 tcagttgtaa tcaaaactcc atcgcatcta atcgttctga ggaaatcatc caagcaatgc 32160 aactggattg tgtttcaagc aggagaggag agggaagaga cggaagaacc atgttaattt 32220 ttattccaaa cgatctcgca gtacttcaaa ttgtagatcg cgcagatggc atctctcgcc 32280 cccactgtgt tggtgaaaaa gcacagctag atcaaaagaa atgcgatttt caaggtgctc 32340 aacggtggct tccagcaaag cctccacgcg cacatccaag aacaaaagaa taccaaaaga 32400 aggagcattt tctaactcct caatcatcat attacattcc tgcaccattc ccagataatt 32460 ttcagctttc cagccttgaa ttattcgtgt cagttcttgt ggtaaatcca atccacacat 32520 tacaaacagg tcccggaggg cgccctccac caccattctt aaacacaccc tcataatgac 32580 aaaatatctt gctcctgtgt cacctgtagc gaattgagaa tggcaacatc aattgacatg 32640 cccttggctc taagttcttc tttaagttct agttgtaaaa actctctcat attatcacca 32700 aactgcttag ccagaagccc cccgggaaca agagcagggg acgctacagt gcagtacaag 32760 cgcagacctc cccaattggc tccagcaaaa acaagattgg aataagcata ttgggaaccg 32820 ccagtaatat catcgaagtt gctggaaata taatcaggca gagtttcttg taaaaattga 32880 ataaaagaaa aatttgccaa aaaaacattc aaaacctctg ggatgcaaat gcaataggtt 32940 accgcgctgc gctccaacat tgttagtttt gaattagtct gcaaaaataa aaaaaaaaac 33000 aagcgtcata tcatagtagc ctgacgaaca gatggataaa tcagtctttc catcacaaga 33060 caagccacag ggtctccagc tcgaccctcg taaaacctgt catcatgatt aaacaacagc 33120 accgaaagtt cctcgcggtg accagcatga ataattcttg atgaagcata caatccagac 33180 atgttagcat cagttaacga gaaaaaacag ccaacatagc ctttgggtat aattatgctt 33240 aatcgtaagt atagcaaagc cacccctcgc ggatacaaag taaaaggcac aggagaataa 33300 aaaatataat tatttctctg ctgctgttca ggcaacgtcg cccccggtcc ctctaaatac 33360 acatacaaag cctcatcagc catggcttac cagacaaagt acagcgggca cacaaagcac 33420 aagctctaaa gtgactctcc aacctctcca caatatatat atacacaagc cctaaactga 33480 cgtaatggga gtaaagtgta aaaaatcccg ccaaacccaa cacacacccc gaaactgcgt 33540 caccagggaa aagtacagtt tcacttccgc aatcccaaca ggcgtaactt cctctttctc 33600 acggtacgtg atatcccact aacttgcaac gtcattttcc cacggtcgca ccgccccttt 33660 tagccgttaa ccccacagcc aatcaccaca cgatccacac tttttaaaat cacctcattt 33720 acatattggc accattccat ctataaggta tattatatag ataga 33765 <210> 50 <211> 33868 <212> DNA <213> Artificial Sequence <220> <223> NG-97 virus genome sequence consisting of a transgene cassette that encodes the cytokine, IFN?, inserted in the region BY. <400> 50 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgctgc caagcttccg agctctcgaa ttcaaaggag gtacccacca tggccttgac 30060 ctttgcttta ctggtggccc tcctggtgct cagctgcaag tcaagctgct ctgtgggctg 30120 tgatctgcct caaacccaca gcctgggtag caggaggacc ttgatgctcc tggcacagat 30180 gaggagaatc tctcttttct cctgcttgaa ggacagacat gactttggat ttccccagga 30240 ggagtttggc aaccagttcc aaaaggctga aaccatccct gtcctccatg agatgatcca 30300 gcagatcttc aatctcttca gcacaaagga ctcatctgct gcttgggatg agaccctcct 30360 agacaaattc tacactgaac tctaccagca gctgaatgac ctggaagcct gtgtgataca 30420 gggggtgggg gtgacagaga ctcccctgat gaaggaggac tccattctgg ctgtgaggaa 30480 atacttccaa agaatcactc tctatctgaa agagaagaaa tacagccctt gtgcctggga 30540 ggttgtcaga gcagaaatca tgagatcttt ttctttgtca acaaacttgc aagaaagttt 30600 aagaagtaag gaatgagcta gcttgactga ctgagataca gcgtaccttc agctcacaga 30660 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 30720 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 30780 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga 30840 ggttttttaa agcaagtaaa acctctacaa atgtggtagt cgtcagctat cctgcaggaa 30900 cttgtttatt tgaaaatcaa ttcacaaaat ccgagtagtt attttgcctc ccccttccca 30960 tttaacagaa tacaccaatc tctccccacg cacagcttta aacatttgga taccattaga 31020 tatagacatg gttttagatt ccacattcca aacagtttca gagcgagcca atctggggtc 31080 agtgatagat aaaaatccat cgggatagtc ttttaaagcg ctttcacagt ccaactgctg 31140 cggatgcgac tccggagtct ggatcacggt catctggaag aagaacgatg ggaatcataa 31200 tccgaaaacg gtatcggacg attgtgtctc atcaaaccca caagcagccg ctgtctgcgt 31260 cgctccgtgc gactgctgtt tatgggatca gggtccacag tgtcctgaag catgatttta 31320 atagccctta acatcaactt tctggtgcga tgcgcgcagc aacgcattct gatttcactc 31380 aaatctttgc agtaggtaca acacattatt acaatattgt ttaataaacc ataattaaaa 31440 gcgctccagc caaaactcat atctgatata atcgcccctg catgaccatc ataccaaagt 31500 ttaatataaa ttaaatgacg ttccctcaaa aacacactac ccacatacat gatctctttt 31560 ggcatgtgca tattaacaat ctgtctgtac catggacaac gttggttaat catgcaaccc 31620 aatataacct tccggaacca cactgccaac accgctcccc cagccatgca ttgaagtgaa 31680 ccctgctgat tacaatgaca atgaagaacc caattctctc gaccgtgaat cacttgagaa 31740 tgaaaaatat ctatagtggc acaacataga cataaatgca tgcatcttct cataattttt 31800 aactcctcag gatttagaaa catatcccag ggaataggaa gctcttgcag aacagtaaag 31860 ctggcagaac aaggaagacc acgaacacaa cttacactat gcatagtcat agtatcacaa 31920 tctggcaaca gcgggtggtc ttcagtcata gaagctcggg tttcattttc ctcacaacgt 31980 ggtaactggg ctctggtgta agggtgatgt ctggcgcatg atgtcgagcg tgcgcgcaac 32040 cttgtcataa tggagttgct tcctgacatt ctcgtatttt gtatagcaaa acgcggccct 32100 ggcagaacac actcttcttc gccttctatc ctgccgctta gcgtgttccg tgtgatagtt 32160 caagtacaac cacactctta agttggtcaa aagaatgctg gcttcagttg taatcaaaac 32220 tccatcgcat ctaatcgttc tgaggaaatc atccaagcaa tgcaactgga ttgtgtttca 32280 agcaggagag gagagggaag agacggaaga accatgttaa tttttattcc aaacgatctc 32340 gcagtacttc aaattgtaga tcgcgcagat ggcatctctc gcccccactg tgttggtgaa 32400 aaagcacagc tagatcaaaa gaaatgcgat tttcaaggtg ctcaacggtg gcttccagca 32460 aagcctccac gcgcacatcc aagaacaaaa gaataccaaa agaaggagca ttttctaact 32520 cctcaatcat catattacat tcctgcacca ttcccagata attttcagct ttccagcctt 32580 gaattattcg tgtcagttct tgtggtaaat ccaatccaca cattacaaac aggtcccgga 32640 gggcgccctc caccaccatt cttaaacaca ccctcataat gacaaaatat cttgctcctg 32700 tgtcacctgt agcgaattga gaatggcaac atcaattgac atgcccttgg ctctaagttc 32760 ttctttaagt tctagttgta aaaactctct catattatca ccaaactgct tagccagaag 32820 ccccccggga acaagagcag gggacgctac agtgcagtac aagcgcagac ctccccaatt 32880 ggctccagca aaaacaagat tggaataagc atattgggaa ccgccagtaa tatcatcgaa 32940 gttgctggaa atataatcag gcagagtttc ttgtaaaaat tgaataaaag aaaaatttgc 33000 caaaaaaaca ttcaaaacct ctgggatgca aatgcaatag gttaccgcgc tgcgctccaa 33060 cattgttagt tttgaattag tctgcaaaaa taaaaaaaaa aacaagcgtc atatcatagt 33120 agcctgacga acagatggat aaatcagtct ttccatcaca agacaagcca cagggtctcc 33180 agctcgaccc tcgtaaaacc tgtcatcatg attaaacaac agcaccgaaa gttcctcgcg 33240 gtgaccagca tgaataattc ttgatgaagc atacaatcca gacatgttag catcagttaa 33300 cgagaaaaaa cagccaacat agcctttggg tataattatg cttaatcgta agtatagcaa 33360 agccacccct cgcggataca aagtaaaagg cacaggagaa taaaaaatat aattatttct 33420 ctgctgctgt tcaggcaacg tcgcccccgg tccctctaaa tacacataca aagcctcatc 33480 agccatggct taccagacaa agtacagcgg gcacacaaag cacaagctct aaagtgactc 33540 tccaacctct ccacaatata tatatacaca agccctaaac tgacgtaatg ggagtaaagt 33600 gtaaaaaatc ccgccaaacc caacacacac cccgaaactg cgtcaccagg gaaaagtaca 33660 gtttcacttc cgcaatccca acaggcgtaa cttcctcttt ctcacggtac gtgatatccc 33720 actaacttgc aacgtcattt tcccacggtc gcaccgcccc ttttagccgt taaccccaca 33780 gccaatcacc acacgatcca cactttttaa aatcacctca tttacatatt ggcaccattc 33840 catctataag gtatattata tagataga 33868 <210> 51 <211> 33132 <212> DNA <213> Artificial Sequence <220> <223> NG-92 virus genome sequence consisting of a transgene cassette that encodes the cytokine, IFN?, inserted in the region BY. <400> 51 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgctgctaa tcctttctct cttcaggcca ccatgaaata 29400 tacaagttat atcttggctt ttcagctctg catcgttttg ggttctcttg gctgttactg 29460 ccaggaccca tatgtaaaag aagcagaaaa ccttaagaaa tattttaatg caggtcattc 29520 agatgtagcg gataatggaa ctcttttctt aggcattttg aagaattgga aagaggagag 29580 tgacagaaaa ataatgcaga gccaaattgt ctccttttac ttcaaacttt ttaaaaactt 29640 taaagatgac cagagcatcc aaaagagtgt ggagaccatc aaggaagaca tgaatgtcaa 29700 gtttttcaat agcaacaaaa agaaacgaga tgacttcgaa aagctgacta attattcggt 29760 aactgacttg aatgtccaac gcaaagcaat acatgaactc atccaagtga tggctgaact 29820 gtcgccagca gctaaaacag ggaagcgaaa aaggagtcag atgctgtttc gaggtcgaag 29880 agcatcccag taagctagct tgactgactg agatacagcg taccttcagc tcacagacat 29940 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 30000 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 30060 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 30120 tttttaaagc aagtaaaacc tctacaaatg tggtcctgca ggaacttgtt tatttgaaaa 30180 tcaattcaca aaatccgagt agttattttg cctccccctt cccatttaac agaatacacc 30240 aatctctccc cacgcacagc tttaaacatt tggataccat tagatataga catggtttta 30300 gattccacat tccaaacagt ttcagagcga gccaatctgg ggtcagtgat agataaaaat 30360 ccatcgggat agtcttttaa agcgctttca cagtccaact gctgcggatg cgactccgga 30420 gtctggatca cggtcatctg gaagaagaac gatgggaatc ataatccgaa aacggtatcg 30480 gacgattgtg tctcatcaaa cccacaagca gccgctgtct gcgtcgctcc gtgcgactgc 30540 tgtttatggg atcagggtcc acagtgtcct gaagcatgat tttaatagcc cttaacatca 30600 actttctggt gcgatgcgcg cagcaacgca ttctgatttc actcaaatct ttgcagtagg 30660 tacaacacat tattacaata ttgtttaata aaccataatt aaaagcgctc cagccaaaac 30720 tcatatctga tataatcgcc cctgcatgac catcatacca aagtttaata taaattaaat 30780 gacgttccct caaaaacaca ctacccacat acatgatctc ttttggcatg tgcatattaa 30840 caatctgtct gtaccatgga caacgttggt taatcatgca acccaatata accttccgga 30900 accacactgc caacaccgct cccccagcca tgcattgaag tgaaccctgc tgattacaat 30960 gacaatgaag aacccaattc tctcgaccgt gaatcacttg agaatgaaaa atatctatag 31020 tggcacaaca tagacataaa tgcatgcatc ttctcataat ttttaactcc tcaggattta 31080 gaaacatatc ccagggaata ggaagctctt gcagaacagt aaagctggca gaacaaggaa 31140 gaccacgaac acaacttaca ctatgcatag tcatagtatc acaatctggc aacagcgggt 31200 ggtcttcagt catagaagct cgggtttcat tttcctcaca acgtggtaac tgggctctgg 31260 tgtaagggtg atgtctggcg catgatgtcg agcgtgcgcg caaccttgtc ataatggagt 31320 tgcttcctga cattctcgta ttttgtatag caaaacgcgg ccctggcaga acacactctt 31380 cttcgccttc tatcctgccg cttagcgtgt tccgtgtgat agttcaagta caaccacact 31440 cttaagttgg tcaaaagaat gctggcttca gttgtaatca aaactccatc gcatctaatc 31500 gttctgagga aatcatccaa gcaatgcaac tggattgtgt ttcaagcagg agaggagagg 31560 gaagagacgg aagaaccatg ttaattttta ttccaaacga tctcgcagta cttcaaattg 31620 tagatcgcgc agatggcatc tctcgccccc actgtgttgg tgaaaaagca cagctagatc 31680 aaaagaaatg cgattttcaa ggtgctcaac ggtggcttcc agcaaagcct ccacgcgcac 31740 atccaagaac aaaagaatac caaaagaagg agcattttct aactcctcaa tcatcatatt 31800 acattcctgc accattccca gataattttc agctttccag ccttgaatta ttcgtgtcag 31860 ttcttgtggt aaatccaatc cacacattac aaacaggtcc cggagggcgc cctccaccac 31920 cattcttaaa cacaccctca taatgacaaa atatcttgct cctgtgtcac ctgtagcgaa 31980 ttgagaatgg caacatcaat tgacatgccc ttggctctaa gttcttcttt aagttctagt 32040 tgtaaaaact ctctcatatt atcaccaaac tgcttagcca gaagcccccc gggaacaaga 32100 gcaggggacg ctacagtgca gtacaagcgc agacctcccc aattggctcc agcaaaaaca 32160 agattggaat aagcatattg ggaaccgcca gtaatatcat cgaagttgct ggaaatataa 32220 tcaggcagag tttcttgtaa aaattgaata aaagaaaaat ttgccaaaaa aacattcaaa 32280 acctctggga tgcaaatgca ataggttacc gcgctgcgct ccaacattgt tagttttgaa 32340 ttagtctgca aaaataaaaa aaaaaacaag cgtcatatca tagtagcctg acgaacagat 32400 ggataaatca gtctttccat cacaagacaa gccacagggt ctccagctcg accctcgtaa 32460 aacctgtcat catgattaaa caacagcacc gaaagttcct cgcggtgacc agcatgaata 32520 attcttgatg aagcatacaa tccagacatg ttagcatcag ttaacgagaa aaaacagcca 32580 acatagcctt tgggtataat tatgcttaat cgtaagtata gcaaagccac ccctcgcgga 32640 tacaaagtaa aaggcacagg agaataaaaa atataattat ttctctgctg ctgttcaggc 32700 aacgtcgccc ccggtccctc taaatacaca tacaaagcct catcagccat ggcttaccag 32760 acaaagtaca gcgggcacac aaagcacaag ctctaaagtg actctccaac ctctccacaa 32820 tatatatata cacaagccct aaactgacgt aatgggagta aagtgtaaaa aatcccgcca 32880 aacccaacac acaccccgaa actgcgtcac cagggaaaag tacagtttca cttccgcaat 32940 cccaacaggc gtaacttcct ctttctcacg gtacgtgata tcccactaac ttgcaacgtc 33000 attttcccac ggtcgcaccg ccccttttag ccgttaaccc cacagccaat caccacacga 33060 tccacacttt ttaaaatcac ctcatttaca tattggcacc attccatcta taaggtatat 33120 tatatagata ga 33132 <210> 52 <211> 33199 <212> DNA <213> Artificial Sequence <220> <223> NG-96 virus genome sequence consisting of a transgene cassette that encodes the cytokine, IFN?, inserted in the region BY. <400> 52 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac ctttaaaggt ttgtggggag 12480 tgcatgcccc ggtaggagaa agagcaaccg tgtctagctt gttaactccg aactcccgcc 12540 tattattact gttggtagct cctttcaccg acagcggtag catcgaccgt aattcctatt 12600 tgggttacct actaaacctg tatcgcgaag ccatagggca aagtcaggtg gacgagcaga 12660 cctatcaaga aattacccaa gtcagtcgcg ctttgggaca ggaagacact ggcagtttgg 12720 aagccactct gaacttcttg cttaccaatc ggtctcaaaa gatccctcct caatatgctc 12780 ttactgcgga ggaggagagg atccttagat atgtgcagca gagcgtggga ttgtttctga 12840 tgcaagaggg ggcaactccg actgcagcac tggacatgac agcgcgaaat atggagccca 12900 gcatgtatgc cagtaaccga cctttcatta acaaactgct ggactacttg cacagagctg 12960 ccgctatgaa ctctgattat ttcaccaatg ccatcttaaa cccgcactgg ctgcccccac 13020 ctggtttcta cacgggcgaa tatgacatgc ccgaccctaa tgacggattt ctgtgggacg 13080 acgtggacag cgatgttttt tcacctcttt ctgatcatcg cacgtggaaa aaggaaggcg 13140 gcgatagaat gcattcttct gcatcgctgt ccggggtcat gggtgctacc gcggctgagc 13200 ccgagtctgc aagtcctttt cctagtctac ccttttctct acacagtgta cgtagcagcg 13260 aagtgggtag aataagtcgc ccgagtttaa tgggcgaaga ggagtatcta aacgattcct 13320 tgctcagacc ggcaagagaa aaaaatttcc caaacaatgg aatagaaagt ttggtggata 13380 aaatgagtag atggaagact tatgctcagg atcacagaga cgagcctggg atcatgggga 13440 ttacaagtag agcgagccgt agacgccagc gccatgacag acagaggggt cttgtgtggg 13500 acgatgagga ttcggccgat gatagcagcg tgctggactt gggtgggaga ggaaggggca 13560 acccgtttgc tcatttgcgc cctcgcttgg gtggtatgtt gtaaaaaaaa ataaaaaaaa 13620 aactcaccaa ggccatggcg acgagcgtac gttcgttctt ctttattatc tgtgtctagt 13680 ataatgaggc gagtcgtgct aggcggagcg gtggtgtatc cggagggtcc tcctccttcg 13740 tacgagagcg tgatgcagca gcagcaggcg acggcggtga tgcaatcccc actggaggct 13800 ccctttgtgc ctccgcgata cctggcacct acggagggca gaaacagcat tcgttattcg 13860 gaactggcac ctcagtacga taccaccagg ttgtatctgg tggacaacaa gtcggcggac 13920 attgcttctc tgaactatca gaatgaccac agcaacttct tgaccacggt ggtgcaaaac 13980 aatgacttta cccctacgga agccagcacc cagaccatta actttgatga acgatcgcgg 14040 tggggcggtc agctaaagac catcatgcat actaacatgc caaacgtgaa cgagtatatg 14100 tttagtaaca agttcaaagc gcgtgtgatg gtgtccagaa aacctcccga cggtgctgca 14160 gttggggata cttatgatca caagcaggat attttgaaat atgagtggtt cgagtttact 14220 ttgccagaag gcaacttttc agttactatg actattgatt tgatgaacaa tgccatcata 14280 gataattact tgaaagtggg tagacagaat ggagtgcttg aaagtgacat tggtgttaag 14340 ttcgacacca ggaacttcaa gctgggatgg gatcccgaaa ccaagttgat catgcctgga 14400 gtgtatacgt atgaagcctt ccatcctgac attgtcttac tgcctggctg cggagtggat 14460 tttaccgaga gtcgtttgag caaccttctt ggtatcagaa aaaaacagcc atttcaagag 14520 ggttttaaga ttttgtatga agatttagaa ggtggtaata ttccggccct cttggatgta 14580 gatgcctatg agaacagtaa gaaagaacaa aaagccaaaa tagaagctgc tacagctgct 14640 gcagaagcta aggcaaacat agttgccagc gactctacaa gggttgctaa cgctggagag 14700 gtcagaggag acaattttgc gccaacacct gttccgactg cagaatcatt attggccgat 14760 gtgtctgaag gaacggacgt gaaactcact attcaacctg tagaaaaaga tagtaagaat 14820 agaagctata atgtgttgga agacaaaatc aacacagcct atcgcagttg gtatctttcg 14880 tacaattatg gcgatcccga aaaaggagtg cgttcctgga cattgctcac cacctcagat 14940 gtcacctgcg gagcagagca ggtctactgg tcgcttccag acatgatgaa ggatcctgtc 15000 actttccgct ccactagaca agtcagtaac taccctgtgg tgggtgcaga gcttatgccc 15060 gtcttctcaa agagcttcta caacgaacaa gctgtgtact cccagcagct ccgccagtcc 15120 acctcgctta cgcacgtctt caaccgcttt cctgagaacc agattttaat ccgtccgccg 15180 gcgcccacca ttaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggaccctg 15240 ccgttgcgca gcagtatccg gggagtccaa cgtgtgaccg ttactgacgc cagacgccgc 15300 acctgtccct acgtgtacaa ggcactgggc atagtcgcac cgcgcgtcct ttcaagccgc 15360 actttctaaa aaaaaaaaaa atgtccattc ttatctcgcc cagtaataac accggttggg 15420 gtctgcgcgc tccaagcaag atgtacggag gcgcacgcaa acgttctacc caacatcctg 15480 tccgtgttcg cggacatttt cgcgctccat ggggcgccct caagggccgc actcgcgttc 15540 gaaccaccgt cgatgatgta atcgatcagg tggttgccga cgcccgtaat tatactccta 15600 ctgcgcctac atctactgtg gatgcagtta ttgacagtgt agtggctgac gctcgcaact 15660 atgctcgacg taagagccgg cgaaggcgca ttgccagacg ccaccgagct accactgcca 15720 tgcgagccgc aagagctctg ctacgaagag ctagacgcgt ggggcgaaga gccatgctta 15780 gggcggccag acgtgcagct tcgggcgcca gcgccggcag gtcccgcagg caagcagccg 15840 ctgtcgcagc ggcgactatt gccgacatgg cccaatcgcg aagaggcaat gtatactggg 15900 tgcgtgacgc tgccaccggt caacgtgtac ccgtgcgcac ccgtccccct cgcacttaga 15960 agatactgag cagtctccga tgttgtgtcc cagcggcgag gatgtccaag cgcaaataca 16020 aggaagaaat gctgcaggtt atcgcacctg aagtctacgg ccaaccgttg aaggatgaaa 16080 aaaaaccccg caaaatcaag cgggttaaaa aggacaaaaa agaagaggaa gatggcgatg 16140 atgggctggc ggagtttgtg cgcgagtttg ccccacggcg acgcgtgcaa tggcgtgggc 16200 gcaaagttcg acatgtgttg agacctggaa cttcggtggt ctttacaccc ggcgagcgtt 16260 caagcgctac ttttaagcgt tcctatgatg aggtgtacgg ggatgatgat attcttgagc 16320 aggcggctga ccgattaggc gagtttgctt atggcaagcg tagtagaata acttccaagg 16380 atgagacagt gtcgataccc ttggatcatg gaaatcccac ccctagtctt aaaccggtca 16440 ctttgcagca agtgttaccc gtaactccgc gaacaggtgt taaacgcgaa ggtgaagatt 16500 tgtatcccac tatgcaactg atggtaccca aacgccagaa gttggaggac gttttggaga 16560 aagtaaaagt ggatccagat attcaacctg aggttaaagt gagacccatt aagcaggtag 16620 cgcctggtct gggggtacaa actgtagaca ttaagattcc cactgaaagt atggaagtgc 16680 aaactgaacc cgcaaagcct actgccacct ccactgaagt gcaaacggat ccatggatgc 16740 ccatgcctat tacaactgac gccgccggtc ccactcgaag atcccgacga aagtacggtc 16800 cagcaagtct gttgatgccc aattatgttg tacacccatc tattattcct actcctggtt 16860 accgaggcac tcgctactat cgcagccgaa acagtacctc ccgccgtcgc cgcaagacac 16920 ctgcaaatcg cagtcgtcgc cgtagacgca caagcaaacc gactcccggc gccctggtgc 16980 ggcaagtgta ccgcaatggt agtgcggaac ctttgacact gccgcgtgcg cgttaccatc 17040 cgagtatcat cacttaatca atgttgccgc tgcctccttg cagatatggc cctcacttgt 17100 cgccttcgcg ttcccatcac tggttaccga ggaagaaact cgcgccgtag aagagggatg 17160 ttgggacgcg gaatgcgacg ctacaggcga cggcgtgcta tccgcaagca attgcggggt 17220 ggttttttac cagccttaat tccaattatc gctgctgcaa ttggcgcgat accaggcata 17280 gcttccgtgg cggttcaggc ctcgcaacga cattgacatt ggaaaaaaac gtataaataa 17340 aaaaaaaaaa atacaatgga ctctgacact cctggtcctg tgactatgtt ttcttagaga 17400 tggaagacat caatttttca tccttggctc cgcgacacgg cacgaagccg tacatgggca 17460 cctggagcga catcggcacg agccaactga acgggggcgc cttcaattgg agcagtatct 17520 ggagcgggct taaaaatttt ggctcaacca taaaaacata cgggaacaaa gcttggaaca 17580 gcagtacagg acaggcgctt agaaataaac ttaaagacca gaacttccaa caaaaagtag 17640 tcgatgggat agcttccggc atcaatggag tggtagattt ggctaaccag gctgtgcaga 17700 aaaagataaa cagtcgtttg gacccgccgc cagcaacccc aggtgaaatg caagtggagg 17760 aagaaattcc tccgccagaa aaacgaggcg acaagcgtcc gcgtcccgat ttggaagaga 17820 cgctggtgac gcgcgtagat gaaccgcctt cttatgagga agcaacgaag cttggaatgc 17880 ccaccactag accgatagcc ccaatggcca ccggggtgat gaaaccttct cagttgcatc 17940 gacccgtcac cttggatttg ccccctcccc ctgctgctac tgctgtaccc gcttctaagc 18000 ctgtcgctgc cccgaaacca gtcgccgtag ccaggtcacg tcccgggggc gctcctcgtc 18060 caaatgcgca ctggcaaaat actctgaaca gcatcgtggg tctaggcgtg caaagtgtaa 18120 aacgccgtcg ctgcttttaa ttaaatatgg agtagcgctt aacttgccta tctgtgtata 18180 tgtgtcatta cacgccgtca cagcagcaga ggaaaaaagg aagaggtcgt gcgtcgacgc 18240 tgagttactt tcaagatggc caccccatcg atgctgcccc aatgggcata catgcacatc 18300 gccggacagg atgcttcgga gtacctgagt ccgggtctgg tgcagttcgc ccgcgccaca 18360 gacacctact tcaatctggg aaataagttt agaaatccca ccgtagcgcc gacccacgat 18420 gtgaccaccg accgtagcca gcggctcatg ttgcgcttcg tgcccgttga ccgggaggac 18480 aatacatact cttacaaagt gcggtacacc ctggccgtgg gcgacaacag agtgctggat 18540 atggccagca cgttctttga cattaggggt gtgttggaca gaggtcccag tttcaaaccc 18600 tattctggta cggcttacaa ctccctggct cctaaaggcg ctccaaatac atctcagtgg 18660 attgcagaag gtgtaaaaaa tacaactggt gaggaacacg taacagaaga ggaaaccaat 18720 actactactt acacttttgg caatgctcct gtaaaagctg aagctgaaat tacaaaagaa 18780 ggactcccag taggtttgga agtttcagat gaagaaagta aaccgattta tgctgataaa 18840 acatatcagc cagaacctca gctgggagat gaaacttgga ctgaccttga tggaaaaacc 18900 gaaaagtatg gaggcagggc tctcaaaccc gatactaaga tgaaaccatg ctacgggtcc 18960 tttgccaaac ctactaatgt gaaaggcggt caggcaaaac aaaaaacaac ggagcagcca 19020 aatcagaaag tcgaatatga tatcgacatg gagttttttg atgcggcatc gcagaaaaca 19080 aacttaagtc ctaaaattgt catgtatgca gaaaatgtaa atttggaaac tccagacact 19140 catgtagtgt acaaacctgg aacagaagac acaagttccg aagctaattt gggacaacaa 19200 tctatgccca acagacccaa ctacattggc ttcagagata actttattgg acttatgtac 19260 tataacagta ctggtaacat gggggtgctg gctggtcaag cgtctcagtt aaatgcagtg 19320 gttgacttgc aggacagaaa cacagaactt tcttaccaac tcttgcttga ctctctgggc 19380 gacagaacca gatactttag catgtggaat caggctgtgg acagttatga tcctgatgta 19440 cgtgttattg aaaatcatgg tgtggaagat gaacttccca actactgttt tccactggac 19500 ggcataggtg ttccaacaac cagttacaaa tcaatagttc caaatggaga caatgcgcct 19560 aattggaagg aacctgaagt aaatggaaca agtgagatcg gacagggtaa tttgtttgcc 19620 atggaaatta accttcaagc caatctatgg cgaagtttcc tttattccaa tgtggctcta 19680 tatctcccag actcgtacaa atacaccccg tccaatgtca ctcttccaga aaacaaaaac 19740 acctacgact acatgaacgg gcgggtggtg ccgccatctc tagtagacac ctatgtgaac 19800 attggtgcca ggtggtctct ggatgccatg gacaatgtca acccattcaa ccaccaccgt 19860 aacgctggct tgcgttaccg atccatgctt ctgggtaacg gacgttatgt gcctttccac 19920 atacaagtgc ctcaaaaatt cttcgctgtt aaaaacctgc tgcttctccc aggctcctac 19980 acttatgagt ggaactttag gaaggatgtg aacatggttc tacagagttc cctcggtaac 20040 gacctgcggg tagatggcgc cagcatcagt ttcacgagca tcaacctcta tgctactttt 20100 ttccccatgg ctcacaacac cgcttccacc cttgaagcca tgctgcggaa tgacaccaat 20160 gatcagtcat tcaacgacta cctatctgca gctaacatgc tctaccccat tcctgccaat 20220 gcaaccaata ttcccatttc cattccttct cgcaactggg cggctttcag aggctggtca 20280 tttaccagac tgaaaaccaa agaaactccc tctttggggt ctggatttga cccctacttt 20340 gtctattctg gttctattcc ctacctggat ggtaccttct acctgaacca cacttttaag 20400 aaggtttcca tcatgtttga ctcttcagtg agctggcctg gaaatgacag gttactatct 20460 cctaacgaat ttgaaataaa gcgcactgtg gatggcgaag gctacaacgt agcccaatgc 20520 aacatgacca aagactggtt cttggtacag atgctcgcca actacaacat cggctatcag 20580 ggcttctaca ttccagaagg atacaaagat cgcatgtatt catttttcag aaacttccag 20640 cccatgagca ggcaggtggt tgatgaggtc aattacaaag acttcaaggc cgtcgccata 20700 ccctaccaac acaacaactc tggctttgtg ggttacatgg ctccgaccat gcgccaaggt 20760 caaccctatc ccgctaacta tccctatcca ctcattggaa caactgccgt aaatagtgtt 20820 acgcagaaaa agttcttgtg tgacagaacc atgtggcgca taccgttctc gagcaacttc 20880 atgtctatgg gggcccttac agacttggga cagaatatgc tctatgccaa ctcagctcat 20940 gctctggaca tgacctttga ggtggatccc atggatgagc ccaccctgct ttatcttctc 21000 ttcgaagttt tcgacgtggt cagagtgcat cagccacacc gcggcatcat cgaggcagtc 21060 tacctgcgta caccgttctc ggccggtaac gctaccacgt aagaagcttc ttgcttcttg 21120 caaatagcag ctgcaaccat ggcctgcgga tcccaaaacg gctccagcga gcaagagctc 21180 agagccattg tccaagacct gggttgcgga ccctattttt tgggaaccta cgataagcgc 21240 ttcccggggt tcatggcccc cgataagctc gcctgtgcca ttgtaaatac ggccggacgt 21300 gagacggggg gagagcactg gttggctttc ggttggaacc cacgttctaa cacctgctac 21360 ctttttgatc cttttggatt ctcggatgat cgtctcaaac agatttacca gtttgaatat 21420 gagggtctcc tgcgccgcag cgctcttgct accaaggacc gctgtattac gctggaaaaa 21480 tctacccaga ccgtgcaggg tccccgttct gccgcctgcg gacttttctg ctgcatgttc 21540 cttcacgcct ttgtgcactg gcctgaccgt cccatggacg gaaaccccac catgaaattg 21600 ctaactggag tgccaaacaa catgcttcat tctcctaaag tccagcccac cctgtgtgac 21660 aatcaaaaag cactctacca ttttcttaat acccattcgc cttattttcg ctcccatcgt 21720 acacacatcg aaagggccac tgcgttcgac cgtatggatg ttcaataatg actcatgtaa 21780 acaacgtgtt caataaacat cactttattt ttttacatgt atcaaggctc tgcattactt 21840 atttatttac aagtcgaatg ggttctgacg agaatcagaa tgacccgcag gcagtgatac 21900 gttgcggaac tgatacttgg gttgccactt gaattcggga atcaccaact tgggaaccgg 21960 tatatcgggc aggatgtcac tccacagctt tctggtcagc tgcaaagctc caagcaggtc 22020 aggagccgaa atcttgaaat cacaattagg accagtgctt tgagcgcgag agttgcggta 22080 caccggattg cagcactgaa acaccatcag cgacggatgt ctcacgcttg ccagcacggt 22140 gggatctgca atcatgccca catccagatc ttcagcattg gcaatgctga acggggtcat 22200 cttgcaggtc tgcctaccca tggcgggcac ccaattaggc ttgtggttgc aatcgcagtg 22260 cagggggatc agtatcatct tggcctgatc ctgtctgatt cctggataca cggctctcat 22320 gaaagcatca tattgcttga aagcctgctg ggctttacta ccctcggtat aaaacatccc 22380 gcaggacctg ctcgaaaact ggttagctgc acagccggca tcattcacac agcagcgggc 22440 gtcattgtta gctatttgca ccacacttct gccccagcgg ttttgggtga ttttggttcg 22500 ctcgggattc tcctttaagg ctcgttgtcc gttctcgctg gccacatcca tctcgataat 22560 ctgctccttc tgaatcataa tattgccatg caggcacttc agcttgccct cataatcatt 22620 gcagccatga ggccacaacg cacagcctgt acattcccaa ttatggtggg cgatctgaga 22680 aaaagaatgt atcattccct gcagaaatct tcccatcatc gtgctcagtg tcttgtgact 22740 agtgaaagtt aactggatgc ctcggtgctc ctcgtttacg tactggtgac agatgcgctt 22800 gtattgttcg tgttgctcag gcattagttt aaaagaggtt ctaagttcgt tatccagcct 22860 gtacttctcc atcagcagac acatcacttc catgcctttc tcccaagcag acaccagggg 22920 caagctaatc ggattcttaa cagtgcaggc agcagctcct ttagccagag ggtcatcttt 22980 agcgatcttc tcaatgcttc ttttgccatc cttctcaacg atgcgcacgg gcgggtagct 23040 gaaacccact gctacaagtt gcgcctcttc tctttcttct tcgctgtctt gactgatgtc 23100 ttgcatgggg atatgtttgg tcttccttgg cttctttttg gggggtatcg gaggaggagg 23160 actgtcgctc cgttccggag acagggagga ttgtgacgtt tcgctcacca ttaccaactg 23220 actgtcggta gaagaacctg accccacacg gcgacaggtg tttctcttcg ggggcagagg 23280 tggaggcgat tgcgaagggc tgcggtccga cctggaaggc ggatgactgg cagaacccct 23340 tccgcgttcg ggggtgtgct ccctgtggcg gtcgcttaac tgatttcctt cgcggctggc 23400 cattgtgttc tcctaggcag agaaacaaca gacatggaaa ctcagccatt gctgtcaaca 23460 tcgccacgag tgccatcaca tctcgtcctc agcgacgagg aaaaggagca gagcttaagc 23520 attccaccgc ccagtcctgc caccacctct accctagaag ataaggaggt cgacgcatct 23580 catgacatgc agaataaaaa agcgaaagag tctgagacag acatcgagca agacccgggc 23640 tatgtgacac cggtggaaca cgaggaagag ttgaaacgct ttctagagag agaggatgaa 23700 aactgcccaa aacaacgagc agataactat caccaagatg ctggaaatag ggatcagaac 23760 accgactacc tcatagggct tgacggggaa gacgcgctcc ttaaacatct agcaagacag 23820 tcgctcatag tcaaggatgc attattggac agaactgaag tgcccatcag tgtggaagag 23880 ctcagccgcg cctacgagct taacctcttt tcacctcgta ctccccccaa acgtcagcca 23940 aacggcacct gcgagccaaa tcctcgctta aacttttatc cagcttttgc tgtgccagaa 24000 gtactggcta cctatcacat cttttttaaa aatcaaaaaa ttccagtctc ctgccgcgct 24060 aatcgcaccc gcgccgatgc cctactcaat ctgggacctg gttcacgctt acctgatata 24120 gcttccttgg aagaggttcc aaagatcttc gagggtctgg gcaataatga gactcgggcc 24180 gcaaatgctc tgcaaaaggg agaaaatggc atggatgagc atcacagcgt tctggtggaa 24240 ttggaaggcg ataatgccag actcgcagta ctcaagcgaa gcatcgaggt cacacacttc 24300 gcatatcccg ctgtcaacct gccccctaaa gtcatgacgg cggtcatgga ccagttactc 24360 attaagcgcg caagtcccct ttcagaagac atgcatgacc cagatgcctg tgatgagggt 24420 aaaccagtgg tcagtgatga gcagctaacc cgatggctgg gcaccgactc tcccagggat 24480 ttggaagagc gtcgcaagct tatgatggcc gtggtgctgg ttaccgtaga actagagtgt 24540 ctccgacgtt tctttaccga ttcagaaacc ttgcgcaaac tcgaagagaa tctgcactac 24600 acttttagac acggctttgt gcggcaggca tgcaagatat ctaacgtgga actcaccaac 24660 ctggtttcct acatgggtat tctgcatgag aatcgcctag gacaaagcgt gctgcacagc 24720 accctgaagg gggaagcccg ccgtgattac atccgcgatt gtgtctatct gtacctgtgc 24780 cacacgtggc aaaccggcat gggtgtatgg cagcaatgtt tagaagaaca gaacttgaaa 24840 gagcttgaca agctcttaca gaaatctctt aaggttctgt ggacagggtt cgacgagcgc 24900 accgtcgctt ccgacctggc agacctcatc ttcccagagc gtctcagggt tactttgcga 24960 aacggattgc ctgactttat gagccagagc atgcttaaca attttcgctc tttcatcctg 25020 gaacgctccg gtatcctgcc cgccacctgc tgcgcactgc cctccgactt tgtgcctctc 25080 acctaccgcg agtgcccccc gccgctatgg agtcactgct acctgttccg tctggccaac 25140 tatctctcct accactcgga tgtgatcgag gatgtgagcg gagacggctt gctggagtgt 25200 cactgccgct gcaatctgtg cacgccccac cggtccctag cttgcaaccc ccagttgatg 25260 agcgaaaccc agataatagg cacctttgaa ttgcaaggcc ccagcagcca aggcgatggg 25320 tcttctcctg ggcaaagttt aaaactgacc ccgggactgt ggacctccgc ctacttgcgc 25380 aagtttgctc cggaagatta ccacccctat gaaatcaagt tctatgagga ccaatcacag 25440 cctccaaagg ccgaactttc ggcctgcgtc atcacccagg gggcaattct ggcccaattg 25500 caagccatcc aaaaatcccg ccaagaattt ctactgaaaa agggtaaggg ggtctacctt 25560 gacccccaga ccggcgagga actcaacaca aggttccctc aggatgtccc aacgacgaga 25620 aaacaagaag ttgaaggtgc agccgccgcc cccagaagat atggaggaag attgggacag 25680 tcaggcagag gaggcggagg aggacagtct ggaggacagt ctggaggaag acagtttgga 25740 ggaggaaaac gaggaggcag aggaggtgga agaagtaacc gccgacaaac agttatcctc 25800 ggctgcggag acaagcaaca gcgctaccat ctccgctccg agtcgaggaa cccggcggcg 25860 tcccagcagt agatgggacg agaccggacg cttcccgaac ccaaccagcg cttccaagac 25920 cggtaagaag gatcggcagg gatacaagtc ctggcggggg cataagaatg ccatcatctc 25980 ctgcttgcat gagtgcgggg gcaacatatc cttcacgcgg cgctacttgc tattccacca 26040 tggggtgaac tttccgcgca atgttttgca ttactaccgt cacctccaca gcccctacta 26100 tagccagcaa atcccggcag tctcgacaga taaagacagc ggcggcgacc tccaacagaa 26160 aaccagcagc ggcagttaga aaatacacaa caagtgcagc aacaggagga ttaaagatta 26220 cagccaacga gccagcgcaa acccgagagt taagaaatcg gatctttcca accctgtatg 26280 ccatcttcca gcagagtcgg ggtcaagagc aggaactgaa aataaaaaac cgatctctgc 26340 gttcgctcac cagaagttgt ttgtatcaca agagcgaaga tcaacttcag cgcactctcg 26400 aggacgccga ggctctcttc aacaagtact gcgcgctgac tcttaaagag taggcagcga 26460 ccgcgcttat tcaaaaaagg cgggaattac atcatcctcg acatgagtaa agaaattccc 26520 acgccttaca tgtggagtta tcaaccccaa atgggattgg cggcaggcgc ctcccaggac 26580 tactccaccc gcatgaattg gctcagcgcc gggccttcta tgatttctcg agttaatgat 26640 atacgcgcct accgaaacca aatacttttg gaacagtcag ctcttaccac cacgccccgc 26700 caacacctta atcccagaaa ttggcccgcc gccctagtgt accaggaaag tcccgctccc 26760 accactgtat tacttcctcg agacgcccag gccgaagtcc aaatgactaa tgcaggtgcg 26820 cagttagctg gcggctccac cctatgtcgt cacaggcctc ggcataatat aaaacgcctg 26880 atgatcagag gccgaggtat ccagctcaac gacgagtcgg tgagctctcc gcttggtcta 26940 cgaccagacg gaatctttca gattgccggc tgcgggagat cttccttcac ccctcgtcag 27000 gctgttctga ctttggaaag ttcgtcttcg caaccccgct cgggcggaat cgggaccgtt 27060 caatttgtgg aggagtttac tccctctgtc tacttcaacc ccttctccgg atctcctggg 27120 cattacccgg acgagttcat accgaacttc gacgcgatta gcgagtcagt ggacggctac 27180 gattgatgtc tggtgacgcg gctgagctat ctcggctgcg acatctagac cactgccgcc 27240 gctttcgctg ctttgcccgg gaactcattg agttcatcta cttcgaactc cccaaggatc 27300 accctcaagg tccggcccac ggagtgcgga tttctatcga aggcaaaata gactctcgcc 27360 tgcaacgaat tttctcccag cggcccgtgc tgatcgagcg agaccaggga aacaccacgg 27420 tttccatcta ctgcatttgt aatcaccccg gattgcatga aagcctttgc tgtcttatgt 27480 gtactgagtt taataaaaac tgaattaaga ctctcctacg gactgccgct tcttcaaccc 27540 ggattttaca accagaagaa cgaaactttt cctgtcgtcc aggactctgt taacttcacc 27600 tttcctactc acaaactaga agctcaacga ctacaccgct tttccagaag cattttccct 27660 actaatacta ctttcaaaac cggaggtgag ctccaaggtc ttcctacaga aaacccttgg 27720 gtggaagcgg gccttgtagt gctaggaatt cttgcgggtg ggcttgtgat tattctttgc 27780 tacctataca caccttgctt cactttctta gtggtgttgt ggtattggtt taaaaaatgg 27840 ggcccatact agtcttgctt gttttacttt cgcttttgga accgggttct gccaattacg 27900 atccatgtct agacttcgac ccagaaaact gcacacttac ttttgcaccc gacacaagcc 27960 gcatctgtgg agttcatcgc ctctcttacg aacttggccc ccaacgacaa aaatttacct 28020 gcatggtggg aatcaacccc atagttatca cccagcaaag tggagatact aagggttgca 28080 ttcactgctc ctgcgattcc atcgagtgca cctacaccct gctgaagacc ctatgcggcc 28140 taagagacct gctaccaatg aattaaaaaa tgattaataa aaaatcactt acttgaaatc 28200 agcaataagg tctctgttga aattttctcc cagcagcacc tcacttccct cttcccaact 28260 ctggtattct aaaccccgtt cagcggcata ctttctccat actttaaagg ggatgtcaaa 28320 ttttagctcc tctcctgtac ccacaatctt catgtctttc ttcccagatg accaagagag 28380 tccggctcag tgactccttc aaccctgtct acccctatga agatgaaagc acctcccaac 28440 acccctttat aaacccaggg tttatttccc caaatggctt cacacaaagc ccaaacggag 28500 ttcttacttt aaaatgttta accccactaa caaccacagg cggatctcta cagctaaaag 28560 tgggaggggg acttacagtg gatgacacca acggtttttt gaaagaaaac ataagtgcca 28620 ccacaccact cgttaagact ggtcactcta taggtttacc actaggagcc ggattgggaa 28680 cgaatgaaaa taaactttgt atcaaattag gacaaggact tacattcaat tcaaacaaca 28740 tttgcattga tgacaatatt aacaccttat ggacaggagt caaccccacc gaagccaact 28800 gtcaaatcat gaactccagt gaatctaatg attgcaaatt aattctaaca ctagttaaaa 28860 ctggagcact agtcactgca tttgtttatg ttataggagt atctaacaat tttaatatgc 28920 taactacaca cagaaatata aattttactg cagagctgtt tttcgattct actggtaatt 28980 tactaactag actctcatcc ctcaaaactc cacttaatca taaatcagga caaaacatgg 29040 ctactggtgc cattactaat gctaaaggtt tcatgcccag cacgactgcc tatcctttca 29100 atgataattc tagagaaaaa gaaaactaca tttacggaac ttgttactac acagctagtg 29160 atcgcactgc ttttcccatt gacatatctg tcatgcttaa ccgaagagca ataaatgacg 29220 agacatcata ttgtattcgt ataacttggt cctggaacac aggagatgcc ccagaggtgc 29280 aaacctctgc tacaacccta gtcacctccc catttacctt ttactacatc agagaagacg 29340 actgacaaat aaagtttgcg atcgctgcta atcctttctc tcttcaggcc accatggcct 29400 tgacctttgc tttactggtg gccctcctgg tgctcagctg caagtcaagc tgctctgtgg 29460 gctgtgatct gcctcaaacc cacagcctgg gtagcaggag gaccttgatg ctcctggcac 29520 agatgaggag aatctctctt ttctcctgct tgaaggacag acatgacttt ggatttcccc 29580 aggaggagtt tggcaaccag ttccaaaagg ctgaaaccat ccctgtcctc catgagatga 29640 tccagcagat cttcaatctc ttcagcacaa aggactcatc tgctgcttgg gatgagaccc 29700 tcctagacaa attctacact gaactctacc agcagctgaa tgacctggaa gcctgtgtga 29760 tacagggggt gggggtgaca gagactcccc tgatgaagga ggactccatt ctggctgtga 29820 ggaaatactt ccaaagaatc actctctatc tgaaagagaa gaaatacagc ccttgtgcct 29880 gggaggttgt cagagcagaa atcatgagat ctttttcttt gtcaacaaac ttgcaagaaa 29940 gtttaagaag taaggaatga gctagcttga ctgactgaga tacagcgtac cttcagctca 30000 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 30060 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 30120 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 30180 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tcctgcagga acttgtttat 30240 ttgaaaatca attcacaaaa tccgagtagt tattttgcct cccccttccc atttaacaga 30300 atacaccaat ctctccccac gcacagcttt aaacatttgg ataccattag atatagacat 30360 ggttttagat tccacattcc aaacagtttc agagcgagcc aatctggggt cagtgataga 30420 taaaaatcca tcgggatagt cttttaaagc gctttcacag tccaactgct gcggatgcga 30480 ctccggagtc tggatcacgg tcatctggaa gaagaacgat gggaatcata atccgaaaac 30540 ggtatcggac gattgtgtct catcaaaccc acaagcagcc gctgtctgcg tcgctccgtg 30600 cgactgctgt ttatgggatc agggtccaca gtgtcctgaa gcatgatttt aatagccctt 30660 aacatcaact ttctggtgcg atgcgcgcag caacgcattc tgatttcact caaatctttg 30720 cagtaggtac aacacattat tacaatattg tttaataaac cataattaaa agcgctccag 30780 ccaaaactca tatctgatat aatcgcccct gcatgaccat cataccaaag tttaatataa 30840 attaaatgac gttccctcaa aaacacacta cccacataca tgatctcttt tggcatgtgc 30900 atattaacaa tctgtctgta ccatggacaa cgttggttaa tcatgcaacc caatataacc 30960 ttccggaacc acactgccaa caccgctccc ccagccatgc attgaagtga accctgctga 31020 ttacaatgac aatgaagaac ccaattctct cgaccgtgaa tcacttgaga atgaaaaata 31080 tctatagtgg cacaacatag acataaatgc atgcatcttc tcataatttt taactcctca 31140 ggatttagaa acatatccca gggaatagga agctcttgca gaacagtaaa gctggcagaa 31200 caaggaagac cacgaacaca acttacacta tgcatagtca tagtatcaca atctggcaac 31260 agcgggtggt cttcagtcat agaagctcgg gtttcatttt cctcacaacg tggtaactgg 31320 gctctggtgt aagggtgatg tctggcgcat gatgtcgagc gtgcgcgcaa ccttgtcata 31380 atggagttgc ttcctgacat tctcgtattt tgtatagcaa aacgcggccc tggcagaaca 31440 cactcttctt cgccttctat cctgccgctt agcgtgttcc gtgtgatagt tcaagtacaa 31500 ccacactctt aagttggtca aaagaatgct ggcttcagtt gtaatcaaaa ctccatcgca 31560 tctaatcgtt ctgaggaaat catccaagca atgcaactgg attgtgtttc aagcaggaga 31620 ggagagggaa gagacggaag aaccatgtta atttttattc caaacgatct cgcagtactt 31680 caaattgtag atcgcgcaga tggcatctct cgcccccact gtgttggtga aaaagcacag 31740 ctagatcaaa agaaatgcga ttttcaaggt gctcaacggt ggcttccagc aaagcctcca 31800 cgcgcacatc caagaacaaa agaataccaa aagaaggagc attttctaac tcctcaatca 31860 tcatattaca ttcctgcacc attcccagat aattttcagc tttccagcct tgaattattc 31920 gtgtcagttc ttgtggtaaa tccaatccac acattacaaa caggtcccgg agggcgccct 31980 ccaccaccat tcttaaacac accctcataa tgacaaaata tcttgctcct gtgtcacctg 32040 tagcgaattg agaatggcaa catcaattga catgcccttg gctctaagtt cttctttaag 32100 ttctagttgt aaaaactctc tcatattatc accaaactgc ttagccagaa gccccccggg 32160 aacaagagca ggggacgcta cagtgcagta caagcgcaga cctccccaat tggctccagc 32220 aaaaacaaga ttggaataag catattggga accgccagta atatcatcga agttgctgga 32280 aatataatca ggcagagttt cttgtaaaaa ttgaataaaa gaaaaatttg ccaaaaaaac 32340 attcaaaacc tctgggatgc aaatgcaata ggttaccgcg ctgcgctcca acattgttag 32400 ttttgaatta gtctgcaaaa ataaaaaaaa aaacaagcgt catatcatag tagcctgacg 32460 aacagatgga taaatcagtc tttccatcac aagacaagcc acagggtctc cagctcgacc 32520 ctcgtaaaac ctgtcatcat gattaaacaa cagcaccgaa agttcctcgc ggtgaccagc 32580 atgaataatt cttgatgaag catacaatcc agacatgtta gcatcagtta acgagaaaaa 32640 acagccaaca tagcctttgg gtataattat gcttaatcgt aagtatagca aagccacccc 32700 tcgcggatac aaagtaaaag gcacaggaga ataaaaaata taattatttc tctgctgctg 32760 ttcaggcaac gtcgcccccg gtccctctaa atacacatac aaagcctcat cagccatggc 32820 ttaccagaca aagtacagcg ggcacacaaa gcacaagctc taaagtgact ctccaacctc 32880 tccacaatat atatatacac aagccctaaa ctgacgtaat gggagtaaag tgtaaaaaat 32940 cccgccaaac ccaacacaca ccccgaaact gcgtcaccag ggaaaagtac agtttcactt 33000 ccgcaatccc aacaggcgta acttcctctt tctcacggta cgtgatatcc cactaacttg 33060 caacgtcatt ttcccacggt cgcaccgccc cttttagccg ttaaccccac agccaatcac 33120 cacacgatcc acacttttta aaatcacctc atttacatat tggcaccatt ccatctataa 33180 ggtatattat atagataga 33199 <210> 53 <211> 33314 <212> DNA <213> Artificial Sequence <220> <223> NG-139 virus genome sequence consisting of a transgene cassette that encodes the cytokine, TNF?, inserted in the region BY. <400> 53 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc gccatgagca ctgaaagcat gatccgggac 29400 gtggagctgg ccgaggaggc gctccccaag aagacagggg ggccccaggg ctccaggcgg 29460 tgcttgttcc tcagcctctt ctccttcctg atcgtggcag gcgccaccac gctcttctgc 29520 ctgctgcact ttggagtgat cggcccccag agggaagagt tccccaggga cctctctcta 29580 atcagccctc tggcccaggc agtcagatca tcttctcgaa ccccgagtga caagcctgta 29640 gcccatgttg tagcaaaccc tcaagctgag gggcagctcc agtggctgaa ccgccgggcc 29700 aatgccctcc tggccaatgg cgtggagctg agagataacc agctggtggt gccatcagag 29760 ggcctgtacc tcatctactc ccaggtcctc ttcaagggcc aaggctgccc ctccacccat 29820 gtgctcctca cccacaccat cagccgcatc gccgtctcct accagaccaa ggtcaacctc 29880 ctctctgcca tcaagagccc ctgccagagg gagaccccag agggggctga ggccaagccc 29940 tggtatgagc ccatctatct gggaggggtc ttccagctgg agaagggtga ccgactcagc 30000 gctgagatca atcggcccga ctatctcgac tttgccgagt ctgggcaggt ctactttggg 30060 atcattgccc tgtgagctag cttgactgac tgagatacag cgtaccttca gctcacagac 30120 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 30180 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 30240 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 30300 gttttttaaa gcaagtaaaa cctctacaaa tgtggtcctg caggaacttg tttatttgaa 30360 aatcaattca caaaatccga gtagttattt tgcctccccc ttcccattta acagaataca 30420 ccaatctctc cccacgcaca gctttaaaca tttggatacc attagatata gacatggttt 30480 tagattccac attccaaaca gtttcagagc gagccaatct ggggtcagtg atagataaaa 30540 atccatcggg atagtctttt aaagcgcttt cacagtccaa ctgctgcgga tgcgactccg 30600 gagtctggat cacggtcatc tggaagaaga acgatgggaa tcataatccg aaaacggtat 30660 cggacgattg tgtctcatca aacccacaag cagccgctgt ctgcgtcgct ccgtgcgact 30720 gctgtttatg ggatcagggt ccacagtgtc ctgaagcatg attttaatag cccttaacat 30780 caactttctg gtgcgatgcg cgcagcaacg cattctgatt tcactcaaat ctttgcagta 30840 ggtacaacac attattacaa tattgtttaa taaaccataa ttaaaagcgc tccagccaaa 30900 actcatatct gatataatcg cccctgcatg accatcatac caaagtttaa tataaattaa 30960 atgacgttcc ctcaaaaaca cactacccac atacatgatc tcttttggca tgtgcatatt 31020 aacaatctgt ctgtaccatg gacaacgttg gttaatcatg caacccaata taaccttccg 31080 gaaccacact gccaacaccg ctcccccagc catgcattga agtgaaccct gctgattaca 31140 atgacaatga agaacccaat tctctcgacc gtgaatcact tgagaatgaa aaatatctat 31200 agtggcacaa catagacata aatgcatgca tcttctcata atttttaact cctcaggatt 31260 tagaaacata tcccagggaa taggaagctc ttgcagaaca gtaaagctgg cagaacaagg 31320 aagaccacga acacaactta cactatgcat agtcatagta tcacaatctg gcaacagcgg 31380 gtggtcttca gtcatagaag ctcgggtttc attttcctca caacgtggta actgggctct 31440 ggtgtaaggg tgatgtctgg cgcatgatgt cgagcgtgcg cgcaaccttg tcataatgga 31500 gttgcttcct gacattctcg tattttgtat agcaaaacgc ggccctggca gaacacactc 31560 ttcttcgcct tctatcctgc cgcttagcgt gttccgtgtg atagttcaag tacaaccaca 31620 ctcttaagtt ggtcaaaaga atgctggctt cagttgtaat caaaactcca tcgcatctaa 31680 tcgttctgag gaaatcatcc aagcaatgca actggattgt gtttcaagca ggagaggaga 31740 gggaagagac ggaagaacca tgttaatttt tattccaaac gatctcgcag tacttcaaat 31800 tgtagatcgc gcagatggca tctctcgccc ccactgtgtt ggtgaaaaag cacagctaga 31860 tcaaaagaaa tgcgattttc aaggtgctca acggtggctt ccagcaaagc ctccacgcgc 31920 acatccaaga acaaaagaat accaaaagaa ggagcatttt ctaactcctc aatcatcata 31980 ttacattcct gcaccattcc cagataattt tcagctttcc agccttgaat tattcgtgtc 32040 agttcttgtg gtaaatccaa tccacacatt acaaacaggt cccggagggc gccctccacc 32100 accattctta aacacaccct cataatgaca aaatatcttg ctcctgtgtc acctgtagcg 32160 aattgagaat ggcaacatca attgacatgc ccttggctct aagttcttct ttaagttcta 32220 gttgtaaaaa ctctctcata ttatcaccaa actgcttagc cagaagcccc ccgggaacaa 32280 gagcagggga cgctacagtg cagtacaagc gcagacctcc ccaattggct ccagcaaaaa 32340 caagattgga ataagcatat tgggaaccgc cagtaatatc atcgaagttg ctggaaatat 32400 aatcaggcag agtttcttgt aaaaattgaa taaaagaaaa atttgccaaa aaaacattca 32460 aaacctctgg gatgcaaatg caataggtta ccgcgctgcg ctccaacatt gttagttttg 32520 aattagtctg caaaaataaa aaaaaaaaca agcgtcatat catagtagcc tgacgaacag 32580 atggataaat cagtctttcc atcacaagac aagccacagg gtctccagct cgaccctcgt 32640 aaaacctgtc atcatgatta aacaacagca ccgaaagttc ctcgcggtga ccagcatgaa 32700 taattcttga tgaagcatac aatccagaca tgttagcatc agttaacgag aaaaaacagc 32760 caacatagcc tttgggtata attatgctta atcgtaagta tagcaaagcc acccctcgcg 32820 gatacaaagt aaaaggcaca ggagaataaa aaatataatt atttctctgc tgctgttcag 32880 gcaacgtcgc ccccggtccc tctaaataca catacaaagc ctcatcagcc atggcttacc 32940 agacaaagta cagcgggcac acaaagcaca agctctaaag tgactctcca acctctccac 33000 aatatatata tacacaagcc ctaaactgac gtaatgggag taaagtgtaa aaaatcccgc 33060 caaacccaac acacaccccg aaactgcgtc accagggaaa agtacagttt cacttccgca 33120 atcccaacag gcgtaacttc ctctttctca cggtacgtga tatcccacta acttgcaacg 33180 tcattttccc acggtcgcac cgcccctttt agccgttaac cccacagcca atcaccacac 33240 gatccacact ttttaaaatc acctcattta catattggca ccattccatc tataaggtat 33300 attatataga taga 33314 <210> 54 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Restriction site insert (BY) <400> 54 gcgatcgcta ccctgcagg 19 <210> 55 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Restriction site insert (BX) <400> 55 gcggccgcta tggccggcc 19 <210> 56 <211> 33770 <212> DNA <213> Artificial Sequence <220> <223> NG-220 virus genome sequence consisting of a transgene cassette that encodes the tumour associated antigen, NY-ESO-1, inserted in the region BY. <400> 56 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct ctaccgggta ggggaggcgc 29400 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca 29460 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt 29520 tctttggtgg ccccttcgcg ccaccttcta cccctcccct agtcaggaag ttcccccccg 29580 ccccgcagct cgcgtcatgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 29640 tgcaaatgga cagcaccgct gagcaatgga agcgggtagg cccttggggc agcggccaat 29700 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 29760 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat 29820 tccgcacgct tcaaaagcgc acgtctgccg cgctgttctc ttcttcctca tctccgggcc 29880 tttcgagatc tttgtcgatc ctaccatcca ctcgacacac ccgccagcgg ccgctgccaa 29940 gcttccgagc tctcgaattc aaaggaggta ccaccatgca ggccgaaggc cggggcacag 30000 ggggttcgac gggcgatgct gatggcccag gaggccctgg cattcctgat ggcccagggg 30060 gcaatgctgg cggcccagga gaggcgggtg ccacgggcgg cagaggtccc cggggcgcag 30120 gggcagcaag ggcctcgggg ccgggaggag gcgccccgcg gggtccgcat ggcggcgcgg 30180 cttcagggct gaatggatgc tgcagatgcg gggccagggg gccggagagc cgcctgcttg 30240 agttctacct cgccatgcct ttcgcgacac ccatggaagc agagctggcc cgcaggagcc 30300 tggcccagga tgccccaccg cttcccgtgc caggggtgct tctgaaggag ttcactgtgt 30360 ccggcaacat actgactatc cgactgactg ctgcagacca ccgccaactg cagctctcca 30420 tcagctcctg tctccagcag ctttccctgt tgatgtggat cacgcagtgc tttctgcccg 30480 tgtttttggc tcagcctccc tcagggcaga ggcgctaagc tagcttgact gactgagata 30540 cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa 30600 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 30660 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 30720 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta 30780 gtcgtcagct atcctgcagg aacttgttta tttgaaaatc aattcacaaa atccgagtag 30840 ttattttgcc tcccccttcc catttaacag aatacaccaa tctctcccca cgcacagctt 30900 taaacatttg gataccatta gatatagaca tggttttaga ttccacattc caaacagttt 30960 cagagcgagc caatctgggg tcagtgatag ataaaaatcc atcgggatag tcttttaaag 31020 cgctttcaca gtccaactgc tgcggatgcg actccggagt ctggatcacg gtcatctgga 31080 agaagaacga tgggaatcat aatccgaaaa cggtatcgga cgattgtgtc tcatcaaacc 31140 cacaagcagc cgctgtctgc gtcgctccgt gcgactgctg tttatgggat cagggtccac 31200 agtgtcctga agcatgattt taatagccct taacatcaac tttctggtgc gatgcgcgca 31260 gcaacgcatt ctgatttcac tcaaatcttt gcagtaggta caacacatta ttacaatatt 31320 gtttaataaa ccataattaa aagcgctcca gccaaaactc atatctgata taatcgcccc 31380 tgcatgacca tcataccaaa gtttaatata aattaaatga cgttccctca aaaacacact 31440 acccacatac atgatctctt ttggcatgtg catattaaca atctgtctgt accatggaca 31500 acgttggtta atcatgcaac ccaatataac cttccggaac cacactgcca acaccgctcc 31560 cccagccatg cattgaagtg aaccctgctg attacaatga caatgaagaa cccaattctc 31620 tcgaccgtga atcacttgag aatgaaaaat atctatagtg gcacaacata gacataaatg 31680 catgcatctt ctcataattt ttaactcctc aggatttaga aacatatccc agggaatagg 31740 aagctcttgc agaacagtaa agctggcaga acaaggaaga ccacgaacac aacttacact 31800 atgcatagtc atagtatcac aatctggcaa cagcgggtgg tcttcagtca tagaagctcg 31860 ggtttcattt tcctcacaac gtggtaactg ggctctggtg taagggtgat gtctggcgca 31920 tgatgtcgag cgtgcgcgca accttgtcat aatggagttg cttcctgaca ttctcgtatt 31980 ttgtatagca aaacgcggcc ctggcagaac acactcttct tcgccttcta tcctgccgct 32040 tagcgtgttc cgtgtgatag ttcaagtaca accacactct taagttggtc aaaagaatgc 32100 tggcttcagt tgtaatcaaa actccatcgc atctaatcgt tctgaggaaa tcatccaagc 32160 aatgcaactg gattgtgttt caagcaggag aggagaggga agagacggaa gaaccatgtt 32220 aatttttatt ccaaacgatc tcgcagtact tcaaattgta gatcgcgcag atggcatctc 32280 tcgcccccac tgtgttggtg aaaaagcaca gctagatcaa aagaaatgcg attttcaagg 32340 tgctcaacgg tggcttccag caaagcctcc acgcgcacat ccaagaacaa aagaatacca 32400 aaagaaggag cattttctaa ctcctcaatc atcatattac attcctgcac cattcccaga 32460 taattttcag ctttccagcc ttgaattatt cgtgtcagtt cttgtggtaa atccaatcca 32520 cacattacaa acaggtcccg gagggcgccc tccaccacca ttcttaaaca caccctcata 32580 atgacaaaat atcttgctcc tgtgtcacct gtagcgaatt gagaatggca acatcaattg 32640 acatgccctt ggctctaagt tcttctttaa gttctagttg taaaaactct ctcatattat 32700 caccaaactg cttagccaga agccccccgg gaacaagagc aggggacgct acagtgcagt 32760 acaagcgcag acctccccaa ttggctccag caaaaacaag attggaataa gcatattggg 32820 aaccgccagt aatatcatcg aagttgctgg aaatataatc aggcagagtt tcttgtaaaa 32880 attgaataaa agaaaaattt gccaaaaaaa cattcaaaac ctctgggatg caaatgcaat 32940 aggttaccgc gctgcgctcc aacattgtta gttttgaatt agtctgcaaa aataaaaaaa 33000 aaaacaagcg tcatatcata gtagcctgac gaacagatgg ataaatcagt ctttccatca 33060 caagacaagc cacagggtct ccagctcgac cctcgtaaaa cctgtcatca tgattaaaca 33120 acagcaccga aagttcctcg cggtgaccag catgaataat tcttgatgaa gcatacaatc 33180 cagacatgtt agcatcagtt aacgagaaaa aacagccaac atagcctttg ggtataatta 33240 tgcttaatcg taagtatagc aaagccaccc ctcgcggata caaagtaaaa ggcacaggag 33300 aataaaaaat ataattattt ctctgctgct gttcaggcaa cgtcgccccc ggtccctcta 33360 aatacacata caaagcctca tcagccatgg cttaccagac aaagtacagc gggcacacaa 33420 agcacaagct ctaaagtgac tctccaacct ctccacaata tatatataca caagccctaa 33480 actgacgtaa tgggagtaaa gtgtaaaaaa tcccgccaaa cccaacacac accccgaaac 33540 tgcgtcacca gggaaaagta cagtttcact tccgcaatcc caacaggcgt aacttcctct 33600 ttctcacggt acgtgatatc ccactaactt gcaacgtcat tttcccacgg tcgcaccgcc 33660 ccttttagcc gttaacccca cagccaatca ccacacgatc cacacttttt aaaatcacct 33720 catttacata ttggcaccat tccatctata aggtatatta tatagataga 33770 <210> 57 <211> 33843 <212> DNA <213> Artificial Sequence <220> <223> NG-217 virus genome sequence consisting of a transgene cassette that encodes the tumour associated antigen, NY-ESO-1, inserted in the region BY <400> 57 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcggctcc cgccagatct agtaatcaat tacggggtca 29400 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 29460 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 29520 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 29580 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 29640 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 29700 tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat 29760 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 29820 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 29880 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 29940 ttagtgaacc gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag 30000 cggccgctgc caagcttccg agctctcgaa ttcaaaggag gtaccaccat gcaggccgaa 30060 ggccggggca cagggggttc gacgggcgat gctgatggcc caggaggccc tggcattcct 30120 gatggcccag ggggcaatgc tggcggccca ggagaggcgg gtgccacggg cggcagaggt 30180 ccccggggcg caggggcagc aagggcctcg gggccgggag gaggcgcccc gcggggtccg 30240 catggcggcg cggcttcagg gctgaatgga tgctgcagat gcggggccag ggggccggag 30300 agccgcctgc ttgagttcta cctcgccatg cctttcgcga cacccatgga agcagagctg 30360 gcccgcagga gcctggccca ggatgcccca ccgcttcccg tgccaggggt gcttctgaag 30420 gagttcactg tgtccggcaa catactgact atccgactga ctgctgcaga ccaccgccaa 30480 ctgcagctct ccatcagctc ctgtctccag cagctttccc tgttgatgtg gatcacgcag 30540 tgctttctgc ccgtgttttt ggctcagcct ccctcagggc agaggcgcta agctagcttg 30600 actgactgag atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt 30660 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct 30720 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt 30780 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc 30840 tacaaatgtg gtagtcgtca gctatcctgc aggaacttgt ttatttgaaa atcaattcac 30900 aaaatccgag tagttatttt gcctccccct tcccatttaa cagaatacac caatctctcc 30960 ccacgcacag ctttaaacat ttggatacca ttagatatag acatggtttt agattccaca 31020 ttccaaacag tttcagagcg agccaatctg gggtcagtga tagataaaaa tccatcggga 31080 tagtctttta aagcgctttc acagtccaac tgctgcggat gcgactccgg agtctggatc 31140 acggtcatct ggaagaagaa cgatgggaat cataatccga aaacggtatc ggacgattgt 31200 gtctcatcaa acccacaagc agccgctgtc tgcgtcgctc cgtgcgactg ctgtttatgg 31260 gatcagggtc cacagtgtcc tgaagcatga ttttaatagc ccttaacatc aactttctgg 31320 tgcgatgcgc gcagcaacgc attctgattt cactcaaatc tttgcagtag gtacaacaca 31380 ttattacaat attgtttaat aaaccataat taaaagcgct ccagccaaaa ctcatatctg 31440 atataatcgc ccctgcatga ccatcatacc aaagtttaat ataaattaaa tgacgttccc 31500 tcaaaaacac actacccaca tacatgatct cttttggcat gtgcatatta acaatctgtc 31560 tgtaccatgg acaacgttgg ttaatcatgc aacccaatat aaccttccgg aaccacactg 31620 ccaacaccgc tcccccagcc atgcattgaa gtgaaccctg ctgattacaa tgacaatgaa 31680 gaacccaatt ctctcgaccg tgaatcactt gagaatgaaa aatatctata gtggcacaac 31740 atagacataa atgcatgcat cttctcataa tttttaactc ctcaggattt agaaacatat 31800 cccagggaat aggaagctct tgcagaacag taaagctggc agaacaagga agaccacgaa 31860 cacaacttac actatgcata gtcatagtat cacaatctgg caacagcggg tggtcttcag 31920 tcatagaagc tcgggtttca ttttcctcac aacgtggtaa ctgggctctg gtgtaagggt 31980 gatgtctggc gcatgatgtc gagcgtgcgc gcaaccttgt cataatggag ttgcttcctg 32040 acattctcgt attttgtata gcaaaacgcg gccctggcag aacacactct tcttcgcctt 32100 ctatcctgcc gcttagcgtg ttccgtgtga tagttcaagt acaaccacac tcttaagttg 32160 gtcaaaagaa tgctggcttc agttgtaatc aaaactccat cgcatctaat cgttctgagg 32220 aaatcatcca agcaatgcaa ctggattgtg tttcaagcag gagaggagag ggaagagacg 32280 gaagaaccat gttaattttt attccaaacg atctcgcagt acttcaaatt gtagatcgcg 32340 cagatggcat ctctcgcccc cactgtgttg gtgaaaaagc acagctagat caaaagaaat 32400 gcgattttca aggtgctcaa cggtggcttc cagcaaagcc tccacgcgca catccaagaa 32460 caaaagaata ccaaaagaag gagcattttc taactcctca atcatcatat tacattcctg 32520 caccattccc agataatttt cagctttcca gccttgaatt attcgtgtca gttcttgtgg 32580 taaatccaat ccacacatta caaacaggtc ccggagggcg ccctccacca ccattcttaa 32640 acacaccctc ataatgacaa aatatcttgc tcctgtgtca cctgtagcga attgagaatg 32700 gcaacatcaa ttgacatgcc cttggctcta agttcttctt taagttctag ttgtaaaaac 32760 tctctcatat tatcaccaaa ctgcttagcc agaagccccc cgggaacaag agcaggggac 32820 gctacagtgc agtacaagcg cagacctccc caattggctc cagcaaaaac aagattggaa 32880 taagcatatt gggaaccgcc agtaatatca tcgaagttgc tggaaatata atcaggcaga 32940 gtttcttgta aaaattgaat aaaagaaaaa tttgccaaaa aaacattcaa aacctctggg 33000 atgcaaatgc aataggttac cgcgctgcgc tccaacattg ttagttttga attagtctgc 33060 aaaaataaaa aaaaaaacaa gcgtcatatc atagtagcct gacgaacaga tggataaatc 33120 agtctttcca tcacaagaca agccacaggg tctccagctc gaccctcgta aaacctgtca 33180 tcatgattaa acaacagcac cgaaagttcc tcgcggtgac cagcatgaat aattcttgat 33240 gaagcataca atccagacat gttagcatca gttaacgaga aaaaacagcc aacatagcct 33300 ttgggtataa ttatgcttaa tcgtaagtat agcaaagcca cccctcgcgg atacaaagta 33360 aaaggcacag gagaataaaa aatataatta tttctctgct gctgttcagg caacgtcgcc 33420 cccggtccct ctaaatacac atacaaagcc tcatcagcca tggcttacca gacaaagtac 33480 agcgggcaca caaagcacaa gctctaaagt gactctccaa cctctccaca atatatatat 33540 acacaagccc taaactgacg taatgggagt aaagtgtaaa aaatcccgcc aaacccaaca 33600 cacaccccga aactgcgtca ccagggaaaa gtacagtttc acttccgcaa tcccaacagg 33660 cgtaacttcc tctttctcac ggtacgtgat atcccactaa cttgcaacgt cattttccca 33720 cggtcgcacc gcccctttta gccgttaacc ccacagccaa tcaccacacg atccacactt 33780 tttaaaatca cctcatttac atattggcac cattccatct ataaggtata ttatatagat 33840 aga 33843 <210> 58 <211> 35361 <212> DNA <213> Artificial Sequence <220> <223> NG-242 virus genome sequence consisting of a transgene cassette encoding an anti-CTLA-4 full length antibody inserted into the region BY. <400> 58 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgta tcatcctctt 29400 cttggtagca acagctacag gtgtccactc ccaggtgcag ctggtggagt ctgggggagg 29460 cgtggtccag cctgggaggt ccctgagact ctcctgtgca gcgtctggat tcaccttcag 29520 tagctatggc atgcactggg tccgccaggc tccaggcaag gggctggagt gggtggcagt 29580 tatatggtat gatggaagta ataaatacta tgcagactcc gtgaagggcc gattcaccat 29640 ctccagagac aattccaaga acacgctgta tctgcaaatg aacagcctga gagccgagga 29700 cacggctgtg tattactgtg cgagagatcc gaggggagct accctttact actactacta 29760 cggtatggac gtctggggcc aagggaccac ggtcaccgtc tcctcagcct ccaccaaggg 29820 cccatcggtc ttccccctgg caccctcctc caagagcacc tctgggggca cagcggccct 29880 gggctgcctg gtcaaggact acttccccga accggtgacg gtgtcgtgga actcaggcgc 29940 cctgaccagc ggcgtgcaca ccttcccggc tgtcctacag tcctcaggac tctactccct 30000 cagcagcgtg gtgaccgtgc cctccagcag cttgggcacc cagacctaca tctgcaacgt 30060 gaatcacaag cccagcaaca ccaaggtcga caagaaagtt gagcccaaat cttgtgacaa 30120 aactcacaca tgcccaccgt gcccagcacc tgagctcctg gggggaccgt cagtcttcct 30180 cttcccccca aaacccaagg acaccctcat gatctcccgg acccctgagg tcacatgcgt 30240 ggtggtggac gtgagccacg aagaccctga ggtcaagttc aactggtacg tggacggcgt 30300 ggaggtgcat aatgccaaga caaagccgcg ggaggagcag tacaacagca cgtaccgtgt 30360 ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa 30420 ggtctccaac aaagccctcc cagcccccat cgagaaaacc atctccaaag ccaaagggca 30480 gccccgagaa ccacaggtgt acaccctgcc cccatcccgg gaggagatga ccaagaacca 30540 ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc gacatcgccg tggagtggga 30600 gagcaatggg cagccggaga acaactacaa gaccacgcct cccgtgctgg actccgacgg 30660 ctccttcttc ctctacagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt 30720 cttctcatgc tccgtgatgc acgaggctct gcacaaccac tacacgcaga agagcctctc 30780 cctgtctccg ggtaaatgat ctagactcga ggtttctaga agttgtctcc tcctgcactg 30840 actgactgac ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 30900 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 30960 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 31020 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 31080 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 31140 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 31200 tggatagttg tggaaagagt caaatggctc ccctcaagcg tattcaacaa ggggctgaag 31260 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt 31320 tcatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt 31380 tcctttgaaa aacacgatga taataacatg gactggacct ggcgcgtgtt ttgcctgctc 31440 gccgtggctc ctggggccca cagcgatatc cagatgaccc agtctccatc ctccctgtct 31500 gcatctgtag gagacagagt caccatcact tgccgggcaa gtcagagcat taacagctat 31560 ttagattggt atcagcagaa accagggaaa gcccctaaac tcctgatcta tgctgcatcc 31620 agtttgcaaa gtggggtccc atcaaggttc agtggcagtg gatctgggac agatttcact 31680 ctcaccatca gcagtctgca acctgaagat tttgcaactt actactgtca acagtattac 31740 agtactccat tcactttcgg ccctgggacc aaagtggaaa tcaaacgaac tgtggctgca 31800 ccatctgtct tcatcttccc gccatctgat gagcagttga aatctggaac tgcttctgtt 31860 gtgtgcctgc tgaataactt ctatcccaga gaggccaaag tacagtggaa ggtggataac 31920 gccctccaat cgggtaactc ccaggagagt gtcacagagc aggacagcaa ggacagcacc 31980 tacagcctca gcagcaccct gacgctgagc aaagcagact acgagaaaca caaagtctac 32040 gcctgcgaag tcacccatca gggcctgagc tcgcccgtca caaagagctt caacagggga 32100 gagtgttaag ctagcttgac tgactgagat acagcgtacc ttcagctcac agacatgata 32160 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 32220 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 32280 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 32340 taaagcaagt aaaacctcta caaatgtggt agtcgtcagc tatcctgcag gaacttgttt 32400 atttgaaaat caattcacaa aatccgagta gttattttgc ctcccccttc ccatttaaca 32460 gaatacacca atctctcccc acgcacagct ttaaacattt ggataccatt agatatagac 32520 atggttttag attccacatt ccaaacagtt tcagagcgag ccaatctggg gtcagtgata 32580 gataaaaatc catcgggata gtcttttaaa gcgctttcac agtccaactg ctgcggatgc 32640 gactccggag tctggatcac ggtcatctgg aagaagaacg atgggaatca taatccgaaa 32700 acggtatcgg acgattgtgt ctcatcaaac ccacaagcag ccgctgtctg cgtcgctccg 32760 tgcgactgct gtttatggga tcagggtcca cagtgtcctg aagcatgatt ttaatagccc 32820 ttaacatcaa ctttctggtg cgatgcgcgc agcaacgcat tctgatttca ctcaaatctt 32880 tgcagtaggt acaacacatt attacaatat tgtttaataa accataatta aaagcgctcc 32940 agccaaaact catatctgat ataatcgccc ctgcatgacc atcataccaa agtttaatat 33000 aaattaaatg acgttccctc aaaaacacac tacccacata catgatctct tttggcatgt 33060 gcatattaac aatctgtctg taccatggac aacgttggtt aatcatgcaa cccaatataa 33120 ccttccggaa ccacactgcc aacaccgctc ccccagccat gcattgaagt gaaccctgct 33180 gattacaatg acaatgaaga acccaattct ctcgaccgtg aatcacttga gaatgaaaaa 33240 tatctatagt ggcacaacat agacataaat gcatgcatct tctcataatt tttaactcct 33300 caggatttag aaacatatcc cagggaatag gaagctcttg cagaacagta aagctggcag 33360 aacaaggaag accacgaaca caacttacac tatgcatagt catagtatca caatctggca 33420 acagcgggtg gtcttcagtc atagaagctc gggtttcatt ttcctcacaa cgtggtaact 33480 gggctctggt gtaagggtga tgtctggcgc atgatgtcga gcgtgcgcgc aaccttgtca 33540 taatggagtt gcttcctgac attctcgtat tttgtatagc aaaacgcggc cctggcagaa 33600 cacactcttc ttcgccttct atcctgccgc ttagcgtgtt ccgtgtgata gttcaagtac 33660 aaccacactc ttaagttggt caaaagaatg ctggcttcag ttgtaatcaa aactccatcg 33720 catctaatcg ttctgaggaa atcatccaag caatgcaact ggattgtgtt tcaagcagga 33780 gaggagaggg aagagacgga agaaccatgt taatttttat tccaaacgat ctcgcagtac 33840 ttcaaattgt agatcgcgca gatggcatct ctcgccccca ctgtgttggt gaaaaagcac 33900 agctagatca aaagaaatgc gattttcaag gtgctcaacg gtggcttcca gcaaagcctc 33960 cacgcgcaca tccaagaaca aaagaatacc aaaagaagga gcattttcta actcctcaat 34020 catcatatta cattcctgca ccattcccag ataattttca gctttccagc cttgaattat 34080 tcgtgtcagt tcttgtggta aatccaatcc acacattaca aacaggtccc ggagggcgcc 34140 ctccaccacc attcttaaac acaccctcat aatgacaaaa tatcttgctc ctgtgtcacc 34200 tgtagcgaat tgagaatggc aacatcaatt gacatgccct tggctctaag ttcttcttta 34260 agttctagtt gtaaaaactc tctcatatta tcaccaaact gcttagccag aagccccccg 34320 ggaacaagag caggggacgc tacagtgcag tacaagcgca gacctcccca attggctcca 34380 gcaaaaacaa gattggaata agcatattgg gaaccgccag taatatcatc gaagttgctg 34440 gaaatataat caggcagagt ttcttgtaaa aattgaataa aagaaaaatt tgccaaaaaa 34500 acattcaaaa cctctgggat gcaaatgcaa taggttaccg cgctgcgctc caacattgtt 34560 agttttgaat tagtctgcaa aaataaaaaa aaaaacaagc gtcatatcat agtagcctga 34620 cgaacagatg gataaatcag tctttccatc acaagacaag ccacagggtc tccagctcga 34680 ccctcgtaaa acctgtcatc atgattaaac aacagcaccg aaagttcctc gcggtgacca 34740 gcatgaataa ttcttgatga agcatacaat ccagacatgt tagcatcagt taacgagaaa 34800 aaacagccaa catagccttt gggtataatt atgcttaatc gtaagtatag caaagccacc 34860 cctcgcggat acaaagtaaa aggcacagga gaataaaaaa tataattatt tctctgctgc 34920 tgttcaggca acgtcgcccc cggtccctct aaatacacat acaaagcctc atcagccatg 34980 gcttaccaga caaagtacag cgggcacaca aagcacaagc tctaaagtga ctctccaacc 35040 tctccacaat atatatatac acaagcccta aactgacgta atgggagtaa agtgtaaaaa 35100 atcccgccaa acccaacaca caccccgaaa ctgcgtcacc agggaaaagt acagtttcac 35160 ttccgcaatc ccaacaggcg taacttcctc tttctcacgg tacgtgatat cccactaact 35220 tgcaacgtca ttttcccacg gtcgcaccgc cccttttagc cgttaacccc acagccaatc 35280 accacacgat ccacactttt taaaatcacc tcatttacat attggcacca ttccatctat 35340 aaggtatatt atatagatag a 35361 <210> 59 <211> 34807 <212> DNA <213> Artificial Sequence <220> <223> NG-165 virus genome sequence consisting of a transgene cassette encoding an anti-VEGF full length antibody inserted into the region BY. <400> 59 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgta tcatcctctt 29400 cttggtagca acagctacag gtgtccactc cgaggttcag ctggtggagt ctggcggtgg 29460 cctggtgcag ccagggggct cactccgttt gtcctgtgca gcttctggct ataccttcac 29520 caactatggt atgaactggg tccgtcaggc cccgggtaag ggcctggaat gggttggatg 29580 gattaacacc tataccggtg aaccgaccta tgctgcggat ttcaaacgtc gttttacttt 29640 ttctctggac acctccaaga gcacagctta cctgcagatg aacagcctgc gcgctgagga 29700 cactgccgtc tattactgtg caaagtaccc gcactattat gggagcagcc actggtattt 29760 cgacgtctgg ggtcaaggaa ccctggtcac cgtctcctcg gcctccacca agggcccatc 29820 ggtcttcccc ctggcaccct cctccaagag cacctctggg ggcacagcgg ccctgggctg 29880 cctggtcaag gactacttcc ccgaaccggt gacggtgtcg tggaactcag gcgccctgac 29940 cagcggcgtg cacaccttcc cggctgtcct acagtcctca ggactctact ccctcagcag 30000 cgtggtgacc gtgccctcca gcagcttggg cacccagacc tacatctgca acgtgaatca 30060 caagcccagc aacaccaagg tcgacaagaa agttgagccc aaatcttgtg acaaaactca 30120 cacatgccca ccgtgcccag cacctgagct cctgggggga ccgtcagtct tcctcttccc 30180 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt 30240 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt 30300 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag 30360 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc 30420 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg 30480 agaaccacag gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag 30540 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa 30600 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt 30660 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc 30720 atgctccgtg atgcacgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc 30780 tccgggtaaa ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga 30840 ggagaaccct ggacctgact ggacctggcg cgtgttttgc ctgctcgccg tggctcctgg 30900 ggcccacagc gatatccaga tgacccagtc cccgagctcc ctgtccgcct ctgtgggcga 30960 tagggtcacc atcacctgca gcgcaagtca ggatattagc aactatttaa actggtatca 31020 acagaaacca ggaaaagctc cgaaagtcct gatttacttc acctcctctc tccactctgg 31080 agtcccttct cgcttctctg gatccggttc tgggacggat ttcactctga ccatcagcag 31140 tctgcagcca gaagacttcg caacttatta ctgtcaacag tatagcaccg tgccgtggac 31200 gtttggacag ggtaccaagg tggagatcaa acgaactgtg gctgcaccat ctgtcttcat 31260 cttcccgcca tctgatgagc agttgaaatc tggaactgct tctgttgtgt gcctgctgaa 31320 taacttctat cccagagagg ccaaagtaca gtggaaggtg gataacgccc tccaatcggg 31380 taactcccag gagagtgtca cagagcagga cagcaaggac agcacctaca gcctcagcag 31440 caccctgacg ctgagcaaag cagactacga gaaacacaaa gtctacgcct gcgaagtcac 31500 ccatcagggc ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt gttaagctag 31560 cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat acattgatga 31620 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga 31680 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg 31740 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa 31800 cctctacaaa tgtggtagtc gtcagctatc ctgcaggaac ttgtttattt gaaaatcaat 31860 tcacaaaatc cgagtagtta ttttgcctcc cccttcccat ttaacagaat acaccaatct 31920 ctccccacgc acagctttaa acatttggat accattagat atagacatgg ttttagattc 31980 cacattccaa acagtttcag agcgagccaa tctggggtca gtgatagata aaaatccatc 32040 gggatagtct tttaaagcgc tttcacagtc caactgctgc ggatgcgact ccggagtctg 32100 gatcacggtc atctggaaga agaacgatgg gaatcataat ccgaaaacgg tatcggacga 32160 ttgtgtctca tcaaacccac aagcagccgc tgtctgcgtc gctccgtgcg actgctgttt 32220 atgggatcag ggtccacagt gtcctgaagc atgattttaa tagcccttaa catcaacttt 32280 ctggtgcgat gcgcgcagca acgcattctg atttcactca aatctttgca gtaggtacaa 32340 cacattatta caatattgtt taataaacca taattaaaag cgctccagcc aaaactcata 32400 tctgatataa tcgcccctgc atgaccatca taccaaagtt taatataaat taaatgacgt 32460 tccctcaaaa acacactacc cacatacatg atctcttttg gcatgtgcat attaacaatc 32520 tgtctgtacc atggacaacg ttggttaatc atgcaaccca atataacctt ccggaaccac 32580 actgccaaca ccgctccccc agccatgcat tgaagtgaac cctgctgatt acaatgacaa 32640 tgaagaaccc aattctctcg accgtgaatc acttgagaat gaaaaatatc tatagtggca 32700 caacatagac ataaatgcat gcatcttctc ataattttta actcctcagg atttagaaac 32760 atatcccagg gaataggaag ctcttgcaga acagtaaagc tggcagaaca aggaagacca 32820 cgaacacaac ttacactatg catagtcata gtatcacaat ctggcaacag cgggtggtct 32880 tcagtcatag aagctcgggt ttcattttcc tcacaacgtg gtaactgggc tctggtgtaa 32940 gggtgatgtc tggcgcatga tgtcgagcgt gcgcgcaacc ttgtcataat ggagttgctt 33000 cctgacattc tcgtattttg tatagcaaaa cgcggccctg gcagaacaca ctcttcttcg 33060 ccttctatcc tgccgcttag cgtgttccgt gtgatagttc aagtacaacc acactcttaa 33120 gttggtcaaa agaatgctgg cttcagttgt aatcaaaact ccatcgcatc taatcgttct 33180 gaggaaatca tccaagcaat gcaactggat tgtgtttcaa gcaggagagg agagggaaga 33240 gacggaagaa ccatgttaat ttttattcca aacgatctcg cagtacttca aattgtagat 33300 cgcgcagatg gcatctctcg cccccactgt gttggtgaaa aagcacagct agatcaaaag 33360 aaatgcgatt ttcaaggtgc tcaacggtgg cttccagcaa agcctccacg cgcacatcca 33420 agaacaaaag aataccaaaa gaaggagcat tttctaactc ctcaatcatc atattacatt 33480 cctgcaccat tcccagataa ttttcagctt tccagccttg aattattcgt gtcagttctt 33540 gtggtaaatc caatccacac attacaaaca ggtcccggag ggcgccctcc accaccattc 33600 ttaaacacac cctcataatg acaaaatatc ttgctcctgt gtcacctgta gcgaattgag 33660 aatggcaaca tcaattgaca tgcccttggc tctaagttct tctttaagtt ctagttgtaa 33720 aaactctctc atattatcac caaactgctt agccagaagc cccccgggaa caagagcagg 33780 ggacgctaca gtgcagtaca agcgcagacc tccccaattg gctccagcaa aaacaagatt 33840 ggaataagca tattgggaac cgccagtaat atcatcgaag ttgctggaaa tataatcagg 33900 cagagtttct tgtaaaaatt gaataaaaga aaaatttgcc aaaaaaacat tcaaaacctc 33960 tgggatgcaa atgcaatagg ttaccgcgct gcgctccaac attgttagtt ttgaattagt 34020 ctgcaaaaat aaaaaaaaaa acaagcgtca tatcatagta gcctgacgaa cagatggata 34080 aatcagtctt tccatcacaa gacaagccac agggtctcca gctcgaccct cgtaaaacct 34140 gtcatcatga ttaaacaaca gcaccgaaag ttcctcgcgg tgaccagcat gaataattct 34200 tgatgaagca tacaatccag acatgttagc atcagttaac gagaaaaaac agccaacata 34260 gcctttgggt ataattatgc ttaatcgtaa gtatagcaaa gccacccctc gcggatacaa 34320 agtaaaaggc acaggagaat aaaaaatata attatttctc tgctgctgtt caggcaacgt 34380 cgcccccggt ccctctaaat acacatacaa agcctcatca gccatggctt accagacaaa 34440 gtacagcggg cacacaaagc acaagctcta aagtgactct ccaacctctc cacaatatat 34500 atatacacaa gccctaaact gacgtaatgg gagtaaagtg taaaaaatcc cgccaaaccc 34560 aacacacacc ccgaaactgc gtcaccaggg aaaagtacag tttcacttcc gcaatcccaa 34620 caggcgtaac ttcctctttc tcacggtacg tgatatccca ctaacttgca acgtcatttt 34680 cccacggtcg caccgcccct tttagccgtt aaccccacag ccaatcacca cacgatccac 34740 actttttaaa atcacctcat ttacatattg gcaccattcc atctataagg tatattatat 34800 agataga 34807 <210> 60 <211> 34792 <212> DNA <213> Artificial Sequence <220> <223> NG-190 virus genome sequence consisting of a transgene cassette encoding an anti-PD-L1 full length antibody inserted into the region BY. <400> 60 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgca ttatcctgtt 29400 tctggttgct actgccactg gagtgcatag cgaggtacag cttgtcgagt ctggaggtgg 29460 gctggtacaa ccaggcggat ccctcagact gtcttgtgcc gcatctggct tcacgttcag 29520 cgatagttgg atacactggg tgaggcaggc accagggaaa ggtctggagt gggtggcttg 29580 gataagccca tatggtggct ccacatatta cgccgactca gtgaagggtc gcttcaccat 29640 ttctgctgat acgtcaaaga acacagccta tctccagatg aactcccttc gtgccgagga 29700 taccgctgtc tactattgtg cccggagaca ttggcctggc gggttcgact actggggaca 29760 ggggactctc gtcactgttt ctgcagcgtc tactaaaggg cccagcgtgt ttcctcttgc 29820 tccctccagt aagtcaacta gtggcggcac cgctgctttg ggctgcctgg tcaaggacta 29880 ttttccagag cctgtaaccg tgagctggaa tagtggagca ttgacctcag gagtgcacac 29940 atttcccgca gtgctgcagt caagcggact gtacagcctt tccagtgtgg tgaccgttcc 30000 ttcctcaagt ctgggcactc aaacctacat ctgcaacgtg aaccacaaac ccagcaatac 30060 caaagtcgac aagaaggttg agccgaagag ctgtgacaaa acgcacacat gccctccatg 30120 tcccgcccca gaactgctcg gtggaccgtc cgtgttcctg tttcctccga agcccaagga 30180 cacactgatg atcagtcgca ccccagaagt cacctgtgtg gtggtggcgg taagccacga 30240 agatcccgag gtcaagttca attggtatgt cgatggggtt gaggtccaca atgccaagac 30300 taagccaagg gaggaacagt atgccagcac ataccgagtc gtgtctgtgc tgaccgttct 30360 gcatcaggac tggctcaacg ggaaagagta caagtgcaaa gtgtccaaca aggcacttcc 30420 tgcccctatc gagaaaacca tcagcaaggc gaaaggtcag cccagagaac cacaagtcta 30480 cacactgcca ccctctcggg acgaactcac aaagaatcag gtgtcactga catgcttggt 30540 taaaggcttt taccccagcg atattgccgt ggaatgggaa tccaatgggc aacctgagaa 30600 caactacaag actacacctc ccgtattgga tagcgatgga agcttcttcc tgtactctaa 30660 gttgacggtt gacaaatcca ggtggcagca gggcaatgtg ttttcttgct ccgtaatgca 30720 tgaagctctg cataatcact atacccagaa atccctctca ctgagtccgg gcaaaggaag 30780 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc 30840 tgactggacc tggcgcgtgt tttgcctgct cgccgtggct cctggggccc acagcgatat 30900 ccaaatgact cagtctccct cttcactgtc tgcaagcgtt ggcgataggg tgacaattac 30960 ctgtagagcg agtcaggacg tttcaaccgc tgtagcctgg taccagcaga aaccgggaaa 31020 agcccctaag ctgctcattt actccgcttc ctttctctat tccggagtgc ctagccgatt 31080 tagtggctct ggttcaggga ctgacttcac actgaccata tccagtctgc aaccagagga 31140 tttcgcgacg tactactgtc agcagtatct gtatcacccc gctacatttg ggcaagggac 31200 aaaagtggag atcaaacgga ctgtagccgc acccagcgtg ttcatctttc caccctctga 31260 tgagcagctt aagagcggta cggcatctgt cgtgtgcttg ctgaacaact tctatcctcg 31320 tgaagccaaa gtccagtgga aggtggacaa tgccttgcag tcaggcaact cacaggaaag 31380 cgtcactgaa caggacagca aggatagtac ctacagcctg tccagcacac tgactctctc 31440 caaagccgac tatgagaagc acaaggttta cgcttgcgaa gtgacccatc aaggccttag 31500 cagtccagtc accaagtcct tcaatcgcgg agagtgctga gctagcttga ctgactgaga 31560 tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac 31620 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 31680 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 31740 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 31800 tagtcgtcag ctatcctgca ggaacttgtt tatttgaaaa tcaattcaca aaatccgagt 31860 agttattttg cctccccctt cccatttaac agaatacacc aatctctccc cacgcacagc 31920 tttaaacatt tggataccat tagatataga catggtttta gattccacat tccaaacagt 31980 ttcagagcga gccaatctgg ggtcagtgat agataaaaat ccatcgggat agtcttttaa 32040 agcgctttca cagtccaact gctgcggatg cgactccgga gtctggatca cggtcatctg 32100 gaagaagaac gatgggaatc ataatccgaa aacggtatcg gacgattgtg tctcatcaaa 32160 cccacaagca gccgctgtct gcgtcgctcc gtgcgactgc tgtttatggg atcagggtcc 32220 acagtgtcct gaagcatgat tttaatagcc cttaacatca actttctggt gcgatgcgcg 32280 cagcaacgca ttctgatttc actcaaatct ttgcagtagg tacaacacat tattacaata 32340 ttgtttaata aaccataatt aaaagcgctc cagccaaaac tcatatctga tataatcgcc 32400 cctgcatgac catcatacca aagtttaata taaattaaat gacgttccct caaaaacaca 32460 ctacccacat acatgatctc ttttggcatg tgcatattaa caatctgtct gtaccatgga 32520 caacgttggt taatcatgca acccaatata accttccgga accacactgc caacaccgct 32580 cccccagcca tgcattgaag tgaaccctgc tgattacaat gacaatgaag aacccaattc 32640 tctcgaccgt gaatcacttg agaatgaaaa atatctatag tggcacaaca tagacataaa 32700 tgcatgcatc ttctcataat ttttaactcc tcaggattta gaaacatatc ccagggaata 32760 ggaagctctt gcagaacagt aaagctggca gaacaaggaa gaccacgaac acaacttaca 32820 ctatgcatag tcatagtatc acaatctggc aacagcgggt ggtcttcagt catagaagct 32880 cgggtttcat tttcctcaca acgtggtaac tgggctctgg tgtaagggtg atgtctggcg 32940 catgatgtcg agcgtgcgcg caaccttgtc ataatggagt tgcttcctga cattctcgta 33000 ttttgtatag caaaacgcgg ccctggcaga acacactctt cttcgccttc tatcctgccg 33060 cttagcgtgt tccgtgtgat agttcaagta caaccacact cttaagttgg tcaaaagaat 33120 gctggcttca gttgtaatca aaactccatc gcatctaatc gttctgagga aatcatccaa 33180 gcaatgcaac tggattgtgt ttcaagcagg agaggagagg gaagagacgg aagaaccatg 33240 ttaattttta ttccaaacga tctcgcagta cttcaaattg tagatcgcgc agatggcatc 33300 tctcgccccc actgtgttgg tgaaaaagca cagctagatc aaaagaaatg cgattttcaa 33360 ggtgctcaac ggtggcttcc agcaaagcct ccacgcgcac atccaagaac aaaagaatac 33420 caaaagaagg agcattttct aactcctcaa tcatcatatt acattcctgc accattccca 33480 gataattttc agctttccag ccttgaatta ttcgtgtcag ttcttgtggt aaatccaatc 33540 cacacattac aaacaggtcc cggagggcgc cctccaccac cattcttaaa cacaccctca 33600 taatgacaaa atatcttgct cctgtgtcac ctgtagcgaa ttgagaatgg caacatcaat 33660 tgacatgccc ttggctctaa gttcttcttt aagttctagt tgtaaaaact ctctcatatt 33720 atcaccaaac tgcttagcca gaagcccccc gggaacaaga gcaggggacg ctacagtgca 33780 gtacaagcgc agacctcccc aattggctcc agcaaaaaca agattggaat aagcatattg 33840 ggaaccgcca gtaatatcat cgaagttgct ggaaatataa tcaggcagag tttcttgtaa 33900 aaattgaata aaagaaaaat ttgccaaaaa aacattcaaa acctctggga tgcaaatgca 33960 ataggttacc gcgctgcgct ccaacattgt tagttttgaa ttagtctgca aaaataaaaa 34020 aaaaaacaag cgtcatatca tagtagcctg acgaacagat ggataaatca gtctttccat 34080 cacaagacaa gccacagggt ctccagctcg accctcgtaa aacctgtcat catgattaaa 34140 caacagcacc gaaagttcct cgcggtgacc agcatgaata attcttgatg aagcatacaa 34200 tccagacatg ttagcatcag ttaacgagaa aaaacagcca acatagcctt tgggtataat 34260 tatgcttaat cgtaagtata gcaaagccac ccctcgcgga tacaaagtaa aaggcacagg 34320 agaataaaaa atataattat ttctctgctg ctgttcaggc aacgtcgccc ccggtccctc 34380 taaatacaca tacaaagcct catcagccat ggcttaccag acaaagtaca gcgggcacac 34440 aaagcacaag ctctaaagtg actctccaac ctctccacaa tatatatata cacaagccct 34500 aaactgacgt aatgggagta aagtgtaaaa aatcccgcca aacccaacac acaccccgaa 34560 actgcgtcac cagggaaaag tacagtttca cttccgcaat cccaacaggc gtaacttcct 34620 ctttctcacg gtacgtgata tcccactaac ttgcaacgtc attttcccac ggtcgcaccg 34680 ccccttttag ccgttaaccc cacagccaat caccacacga tccacacttt ttaaaatcac 34740 ctcatttaca tattggcacc attccatcta taaggtatat tatatagata ga 34792 <210> 61 <211> 34792 <212> DNA <213> Artificial Sequence <220> <223> NG-221 virus genome sequence consisting of the EnAd genome with a transgene cassette that encodes an anti-PD-L1 ScFv with a C-terminal His6 tag, inserted in the region BY. <400> 61 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgca ttatcctgtt 29400 tctggttgct actgccactg gagtgcatag cgaggtacag cttgtcgagt ctggaggtgg 29460 gctggtacaa ccaggcggat ccctcagact gtcttgtgcc gcatctggct tcacgttcag 29520 cgatagttgg atacactggg tgaggcaggc accagggaaa ggtctggagt gggtggcttg 29580 gataagccca tatggtggct ccacatatta cgccgactca gtgaagggtc gcttcaccat 29640 ttctgctgat acgtcaaaga acacagccta tctccagatg aactcccttc gtgccgagga 29700 taccgctgtc tactattgtg cccggagaca ttggcctggc gggttcgact actggggaca 29760 ggggactctc gtcactgttt ctgcagcgtc tactaaaggg cccagcgtgt ttcctcttgc 29820 tccctccagt aagtcaacta gtggcggcac cgctgctttg ggctgcctgg tcaaggacta 29880 ttttccagag cctgtaaccg tgagctggaa tagtggagca ttgacctcag gagtgcacac 29940 atttcccgca gtgctgcagt caagcggact gtacagcctt tccagtgtgg tgaccgttcc 30000 ttcctcaagt ctgggcactc aaacctacat ctgcaacgtg aaccacaaac ccagcaatac 30060 caaagtcgac aagaaggttg agccgaagag ctgtgacaaa acgcacacat gccctccatg 30120 tcccgcccca gaactgctcg gtggaccgtc cgtgttcctg tttcctccga agcccaagga 30180 cacactgatg atcagtcgca ccccagaagt cacctgtgtg gtggtggcgg taagccacga 30240 agatcccgag gtcaagttca attggtatgt cgatggggtt gaggtccaca atgccaagac 30300 taagccaagg gaggaacagt atgccagcac ataccgagtc gtgtctgtgc tgaccgttct 30360 gcatcaggac tggctcaacg ggaaagagta caagtgcaaa gtgtccaaca aggcacttcc 30420 tgcccctatc gagaaaacca tcagcaaggc gaaaggtcag cccagagaac cacaagtcta 30480 cacactgcca ccctctcggg acgaactcac aaagaatcag gtgtcactga catgcttggt 30540 taaaggcttt taccccagcg atattgccgt ggaatgggaa tccaatgggc aacctgagaa 30600 caactacaag actacacctc ccgtattgga tagcgatgga agcttcttcc tgtactctaa 30660 gttgacggtt gacaaatcca ggtggcagca gggcaatgtg ttttcttgct ccgtaatgca 30720 tgaagctctg cataatcact atacccagaa atccctctca ctgagtccgg gcaaaggaag 30780 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc 30840 tgactggacc tggcgcgtgt tttgcctgct cgccgtggct cctggggccc acagcgatat 30900 ccaaatgact cagtctccct cttcactgtc tgcaagcgtt ggcgataggg tgacaattac 30960 ctgtagagcg agtcaggacg tttcaaccgc tgtagcctgg taccagcaga aaccgggaaa 31020 agcccctaag ctgctcattt actccgcttc ctttctctat tccggagtgc ctagccgatt 31080 tagtggctct ggttcaggga ctgacttcac actgaccata tccagtctgc aaccagagga 31140 tttcgcgacg tactactgtc agcagtatct gtatcacccc gctacatttg ggcaagggac 31200 aaaagtggag atcaaacgga ctgtagccgc acccagcgtg ttcatctttc caccctctga 31260 tgagcagctt aagagcggta cggcatctgt cgtgtgcttg ctgaacaact tctatcctcg 31320 tgaagccaaa gtccagtgga aggtggacaa tgccttgcag tcaggcaact cacaggaaag 31380 cgtcactgaa caggacagca aggatagtac ctacagcctg tccagcacac tgactctctc 31440 caaagccgac tatgagaagc acaaggttta cgcttgcgaa gtgacccatc aaggccttag 31500 cagtccagtc accaagtcct tcaatcgcgg agagtgctga gctagcttga ctgactgaga 31560 tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg gacaaaccac 31620 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 31680 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 31740 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 31800 tagtcgtcag ctatcctgca ggaacttgtt tatttgaaaa tcaattcaca aaatccgagt 31860 agttattttg cctccccctt cccatttaac agaatacacc aatctctccc cacgcacagc 31920 tttaaacatt tggataccat tagatataga catggtttta gattccacat tccaaacagt 31980 ttcagagcga gccaatctgg ggtcagtgat agataaaaat ccatcgggat agtcttttaa 32040 agcgctttca cagtccaact gctgcggatg cgactccgga gtctggatca cggtcatctg 32100 gaagaagaac gatgggaatc ataatccgaa aacggtatcg gacgattgtg tctcatcaaa 32160 cccacaagca gccgctgtct gcgtcgctcc gtgcgactgc tgtttatggg atcagggtcc 32220 acagtgtcct gaagcatgat tttaatagcc cttaacatca actttctggt gcgatgcgcg 32280 cagcaacgca ttctgatttc actcaaatct ttgcagtagg tacaacacat tattacaata 32340 ttgtttaata aaccataatt aaaagcgctc cagccaaaac tcatatctga tataatcgcc 32400 cctgcatgac catcatacca aagtttaata taaattaaat gacgttccct caaaaacaca 32460 ctacccacat acatgatctc ttttggcatg tgcatattaa caatctgtct gtaccatgga 32520 caacgttggt taatcatgca acccaatata accttccgga accacactgc caacaccgct 32580 cccccagcca tgcattgaag tgaaccctgc tgattacaat gacaatgaag aacccaattc 32640 tctcgaccgt gaatcacttg agaatgaaaa atatctatag tggcacaaca tagacataaa 32700 tgcatgcatc ttctcataat ttttaactcc tcaggattta gaaacatatc ccagggaata 32760 ggaagctctt gcagaacagt aaagctggca gaacaaggaa gaccacgaac acaacttaca 32820 ctatgcatag tcatagtatc acaatctggc aacagcgggt ggtcttcagt catagaagct 32880 cgggtttcat tttcctcaca acgtggtaac tgggctctgg tgtaagggtg atgtctggcg 32940 catgatgtcg agcgtgcgcg caaccttgtc ataatggagt tgcttcctga cattctcgta 33000 ttttgtatag caaaacgcgg ccctggcaga acacactctt cttcgccttc tatcctgccg 33060 cttagcgtgt tccgtgtgat agttcaagta caaccacact cttaagttgg tcaaaagaat 33120 gctggcttca gttgtaatca aaactccatc gcatctaatc gttctgagga aatcatccaa 33180 gcaatgcaac tggattgtgt ttcaagcagg agaggagagg gaagagacgg aagaaccatg 33240 ttaattttta ttccaaacga tctcgcagta cttcaaattg tagatcgcgc agatggcatc 33300 tctcgccccc actgtgttgg tgaaaaagca cagctagatc aaaagaaatg cgattttcaa 33360 ggtgctcaac ggtggcttcc agcaaagcct ccacgcgcac atccaagaac aaaagaatac 33420 caaaagaagg agcattttct aactcctcaa tcatcatatt acattcctgc accattccca 33480 gataattttc agctttccag ccttgaatta ttcgtgtcag ttcttgtggt aaatccaatc 33540 cacacattac aaacaggtcc cggagggcgc cctccaccac cattcttaaa cacaccctca 33600 taatgacaaa atatcttgct cctgtgtcac ctgtagcgaa ttgagaatgg caacatcaat 33660 tgacatgccc ttggctctaa gttcttcttt aagttctagt tgtaaaaact ctctcatatt 33720 atcaccaaac tgcttagcca gaagcccccc gggaacaaga gcaggggacg ctacagtgca 33780 gtacaagcgc agacctcccc aattggctcc agcaaaaaca agattggaat aagcatattg 33840 ggaaccgcca gtaatatcat cgaagttgct ggaaatataa tcaggcagag tttcttgtaa 33900 aaattgaata aaagaaaaat ttgccaaaaa aacattcaaa acctctggga tgcaaatgca 33960 ataggttacc gcgctgcgct ccaacattgt tagttttgaa ttagtctgca aaaataaaaa 34020 aaaaaacaag cgtcatatca tagtagcctg acgaacagat ggataaatca gtctttccat 34080 cacaagacaa gccacagggt ctccagctcg accctcgtaa aacctgtcat catgattaaa 34140 caacagcacc gaaagttcct cgcggtgacc agcatgaata attcttgatg aagcatacaa 34200 tccagacatg ttagcatcag ttaacgagaa aaaacagcca acatagcctt tgggtataat 34260 tatgcttaat cgtaagtata gcaaagccac ccctcgcgga tacaaagtaa aaggcacagg 34320 agaataaaaa atataattat ttctctgctg ctgttcaggc aacgtcgccc ccggtccctc 34380 taaatacaca tacaaagcct catcagccat ggcttaccag acaaagtaca gcgggcacac 34440 aaagcacaag ctctaaagtg actctccaac ctctccacaa tatatatata cacaagccct 34500 aaactgacgt aatgggagta aagtgtaaaa aatcccgcca aacccaacac acaccccgaa 34560 actgcgtcac cagggaaaag tacagtttca cttccgcaat cccaacaggc gtaacttcct 34620 ctttctcacg gtacgtgata tcccactaac ttgcaacgtc attttcccac ggtcgcaccg 34680 ccccttttag ccgttaaccc cacagccaat caccacacga tccacacttt ttaaaatcac 34740 ctcatttaca tattggcacc attccatcta taaggtatat tatatagata ga 34792 <210> 62 <211> 35501 <212> DNA <213> Artificial Sequence <220> <223> NG-258 virus genome sequence consisting of a transgene cassette encoding an anti-VEGF full length antibody inserted into the region BY <400> 62 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta ctgcggccgc 28200 tatggccggc ctgaaatcag caataaggtc tctgttgaaa ttttctccca gcagcacctc 28260 acttccctct tcccaactct ggtattctaa accccgttca gcggcatact ttctccatac 28320 tttaaagggg atgtcaaatt ttagctcctc tcctgtaccc acaatcttca tgtctttctt 28380 cccagatgac caagagagtc cggctcagtg actccttcaa ccctgtctac ccctatgaag 28440 atgaaagcac ctcccaacac ccctttataa acccagggtt tatttcccca aatggcttca 28500 cacaaagccc aaacggagtt cttactttaa aatgtttaac cccactaaca accacaggcg 28560 gatctctaca gctaaaagtg ggagggggac ttacagtgga tgacaccaac ggttttttga 28620 aagaaaacat aagtgccacc acaccactcg ttaagactgg tcactctata ggtttaccac 28680 taggagccgg attgggaacg aatgaaaata aactttgtat caaattagga caaggactta 28740 cattcaattc aaacaacatt tgcattgatg acaatattaa caccttatgg acaggagtca 28800 accccaccga agccaactgt caaatcatga actccagtga atctaatgat tgcaaattaa 28860 ttctaacact agttaaaact ggagcactag tcactgcatt tgtttatgtt ataggagtat 28920 ctaacaattt taatatgcta actacacaca gaaatataaa ttttactgca gagctgtttt 28980 tcgattctac tggtaattta ctaactagac tctcatccct caaaactcca cttaatcata 29040 aatcaggaca aaacatggct actggtgcca ttactaatgc taaaggtttc atgcccagca 29100 cgactgccta tcctttcaat gataattcta gagaaaaaga aaactacatt tacggaactt 29160 gttactacac agctagtgat cgcactgctt ttcccattga catatctgtc atgcttaacc 29220 gaagagcaat aaatgacgag acatcatatt gtattcgtat aacttggtcc tggaacacag 29280 gagatgcccc agaggtgcaa acctctgcta caaccctagt cacctcccca tttacctttt 29340 actacatcag agaagacgac tgacaaataa agtttgcgat cgcggctccc gccagatcta 29400 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 29460 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 29520 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 29580 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 29640 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 29700 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tgctgatgcg 29760 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 29820 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 29880 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 29940 ctatataagc agagctggtt tagtgaaccg tcagatcaga tctttgtcga tcctaccatc 30000 cactcgacac acccgccagc ggccgctgcc aagcttccga gctctcgaat tcaaaggagg 30060 tacccaccat gggatggagc tgtatcatcc tcttcttggt agcaacagct acaggtgtcc 30120 actccgaggt tcagctggtg gagtctggcg gtggcctggt gcagccaggg ggctcactcc 30180 gtttgtcctg tgcagcttct ggctatacct tcaccaacta tggtatgaac tgggtccgtc 30240 aggccccggg taagggcctg gaatgggttg gatggattaa cacctatacc ggtgaaccga 30300 cctatgctgc ggatttcaaa cgtcgtttta ctttttctct ggacacctcc aagagcacag 30360 cttacctgca gatgaacagc ctgcgcgctg aggacactgc cgtctattac tgtgcaaagt 30420 acccgcacta ttatgggagc agccactggt atttcgacgt ctggggtcaa ggaaccctgg 30480 tcaccgtctc ctcggcctcc accaagggcc catcggtctt ccccctggca ccctcctcca 30540 agagcacctc tgggggcaca gcggccctgg gctgcctggt caaggactac ttccccgaac 30600 cggtgacggt gtcgtggaac tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg 30660 tcctacagtc ctcaggactc tactccctca gcagcgtggt gaccgtgccc tccagcagct 30720 tgggcaccca gacctacatc tgcaacgtga atcacaagcc cagcaacacc aaggtcgaca 30780 agaaagttga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc ccagcacctg 30840 agctcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac accctcatga 30900 tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa gaccctgagg 30960 tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca aagccgcggg 31020 aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg caccaggact 31080 ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca gcccccatcg 31140 agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac accctgcccc 31200 catcccggga ggagatgacc aagaaccagg tcagcctgac ctgcctggtc aaaggcttct 31260 atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac aactacaaga 31320 ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag ctcaccgtgg 31380 acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcac gaggctctgc 31440 acaaccacta cacgcagaag agcctctccc tgtctccggg taaaggaagc ggagctacta 31500 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct gactggacct 31560 ggcgcgtgtt ttgcctgctc gccgtggctc ctggggccca cagcgatatc cagatgaccc 31620 agtccccgag ctccctgtcc gcctctgtgg gcgatagggt caccatcacc tgcagcgcaa 31680 gtcaggatat tagcaactat ttaaactggt atcaacagaa accaggaaaa gctccgaaag 31740 tcctgattta cttcacctcc tctctccact ctggagtccc ttctcgcttc tctggatccg 31800 gttctgggac ggatttcact ctgaccatca gcagtctgca gccagaagac ttcgcaactt 31860 attactgtca acagtatagc accgtgccgt ggacgtttgg acagggtacc aaggtggaga 31920 tcaaacgaac tgtggctgca ccatctgtct tcatcttccc gccatctgat gagcagttga 31980 aatctggaac tgcttctgtt gtgtgcctgc tgaataactt ctatcccaga gaggccaaag 32040 tacagtggaa ggtggataac gccctccaat cgggtaactc ccaggagagt gtcacagagc 32100 aggacagcaa ggacagcacc tacagcctca gcagcaccct gacgctgagc aaagcagact 32160 acgagaaaca caaagtctac gcctgcgaag tcacccatca gggcctgagc tcgcccgtca 32220 caaagagctt caacagggga gagtgttaag ctagcttgac tgactgagat acagcgtacc 32280 ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 32340 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 32400 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg 32460 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt agtcgtcagc 32520 tatcctgcag gaacttgttt atttgaaaat caattcacaa aatccgagta gttattttgc 32580 ctcccccttc ccatttaaca gaatacacca atctctcccc acgcacagct ttaaacattt 32640 ggataccatt agatatagac atggttttag attccacatt ccaaacagtt tcagagcgag 32700 ccaatctggg gtcagtgata gataaaaatc catcgggata gtcttttaaa gcgctttcac 32760 agtccaactg ctgcggatgc gactccggag tctggatcac ggtcatctgg aagaagaacg 32820 atgggaatca taatccgaaa acggtatcgg acgattgtgt ctcatcaaac ccacaagcag 32880 ccgctgtctg cgtcgctccg tgcgactgct gtttatggga tcagggtcca cagtgtcctg 32940 aagcatgatt ttaatagccc ttaacatcaa ctttctggtg cgatgcgcgc agcaacgcat 33000 tctgatttca ctcaaatctt tgcagtaggt acaacacatt attacaatat tgtttaataa 33060 accataatta aaagcgctcc agccaaaact catatctgat ataatcgccc ctgcatgacc 33120 atcataccaa agtttaatat aaattaaatg acgttccctc aaaaacacac tacccacata 33180 catgatctct tttggcatgt gcatattaac aatctgtctg taccatggac aacgttggtt 33240 aatcatgcaa cccaatataa ccttccggaa ccacactgcc aacaccgctc ccccagccat 33300 gcattgaagt gaaccctgct gattacaatg acaatgaaga acccaattct ctcgaccgtg 33360 aatcacttga gaatgaaaaa tatctatagt ggcacaacat agacataaat gcatgcatct 33420 tctcataatt tttaactcct caggatttag aaacatatcc cagggaatag gaagctcttg 33480 cagaacagta aagctggcag aacaaggaag accacgaaca caacttacac tatgcatagt 33540 catagtatca caatctggca acagcgggtg gtcttcagtc atagaagctc gggtttcatt 33600 ttcctcacaa cgtggtaact gggctctggt gtaagggtga tgtctggcgc atgatgtcga 33660 gcgtgcgcgc aaccttgtca taatggagtt gcttcctgac attctcgtat tttgtatagc 33720 aaaacgcggc cctggcagaa cacactcttc ttcgccttct atcctgccgc ttagcgtgtt 33780 ccgtgtgata gttcaagtac aaccacactc ttaagttggt caaaagaatg ctggcttcag 33840 ttgtaatcaa aactccatcg catctaatcg ttctgaggaa atcatccaag caatgcaact 33900 ggattgtgtt tcaagcagga gaggagaggg aagagacgga agaaccatgt taatttttat 33960 tccaaacgat ctcgcagtac ttcaaattgt agatcgcgca gatggcatct ctcgccccca 34020 ctgtgttggt gaaaaagcac agctagatca aaagaaatgc gattttcaag gtgctcaacg 34080 gtggcttcca gcaaagcctc cacgcgcaca tccaagaaca aaagaatacc aaaagaagga 34140 gcattttcta actcctcaat catcatatta cattcctgca ccattcccag ataattttca 34200 gctttccagc cttgaattat tcgtgtcagt tcttgtggta aatccaatcc acacattaca 34260 aacaggtccc ggagggcgcc ctccaccacc attcttaaac acaccctcat aatgacaaaa 34320 tatcttgctc ctgtgtcacc tgtagcgaat tgagaatggc aacatcaatt gacatgccct 34380 tggctctaag ttcttcttta agttctagtt gtaaaaactc tctcatatta tcaccaaact 34440 gcttagccag aagccccccg ggaacaagag caggggacgc tacagtgcag tacaagcgca 34500 gacctcccca attggctcca gcaaaaacaa gattggaata agcatattgg gaaccgccag 34560 taatatcatc gaagttgctg gaaatataat caggcagagt ttcttgtaaa aattgaataa 34620 aagaaaaatt tgccaaaaaa acattcaaaa cctctgggat gcaaatgcaa taggttaccg 34680 cgctgcgctc caacattgtt agttttgaat tagtctgcaa aaataaaaaa aaaaacaagc 34740 gtcatatcat agtagcctga cgaacagatg gataaatcag tctttccatc acaagacaag 34800 ccacagggtc tccagctcga ccctcgtaaa acctgtcatc atgattaaac aacagcaccg 34860 aaagttcctc gcggtgacca gcatgaataa ttcttgatga agcatacaat ccagacatgt 34920 tagcatcagt taacgagaaa aaacagccaa catagccttt gggtataatt atgcttaatc 34980 gtaagtatag caaagccacc cctcgcggat acaaagtaaa aggcacagga gaataaaaaa 35040 tataattatt tctctgctgc tgttcaggca acgtcgcccc cggtccctct aaatacacat 35100 acaaagcctc atcagccatg gcttaccaga caaagtacag cgggcacaca aagcacaagc 35160 tctaaagtga ctctccaacc tctccacaat atatatatac acaagcccta aactgacgta 35220 atgggagtaa agtgtaaaaa atcccgccaa acccaacaca caccccgaaa ctgcgtcacc 35280 agggaaaagt acagtttcac ttccgcaatc ccaacaggcg taacttcctc tttctcacgg 35340 tacgtgatat cccactaact tgcaacgtca ttttcccacg gtcgcaccgc cccttttagc 35400 cgttaacccc acagccaatc accacacgat ccacactttt taaaatcacc tcatttacat 35460 attggcacca ttccatctat aaggtatatt atatagatag a 35501 <210> 63 <211> 32364 <212> DNA <213> Artificial Sequence <220> <223> NG-185 virus genome sequence consisting of the EnAd genome with unique restriction sites inserted into the BX and BY regions. <400> 63 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta ctgcggccgc 28200 tatggccggc ctgaaatcag caataaggtc tctgttgaaa ttttctccca gcagcacctc 28260 acttccctct tcccaactct ggtattctaa accccgttca gcggcatact ttctccatac 28320 tttaaagggg atgtcaaatt ttagctcctc tcctgtaccc acaatcttca tgtctttctt 28380 cccagatgac caagagagtc cggctcagtg actccttcaa ccctgtctac ccctatgaag 28440 atgaaagcac ctcccaacac ccctttataa acccagggtt tatttcccca aatggcttca 28500 cacaaagccc aaacggagtt cttactttaa aatgtttaac cccactaaca accacaggcg 28560 gatctctaca gctaaaagtg ggagggggac ttacagtgga tgacaccaac ggttttttga 28620 aagaaaacat aagtgccacc acaccactcg ttaagactgg tcactctata ggtttaccac 28680 taggagccgg attgggaacg aatgaaaata aactttgtat caaattagga caaggactta 28740 cattcaattc aaacaacatt tgcattgatg acaatattaa caccttatgg acaggagtca 28800 accccaccga agccaactgt caaatcatga actccagtga atctaatgat tgcaaattaa 28860 ttctaacact agttaaaact ggagcactag tcactgcatt tgtttatgtt ataggagtat 28920 ctaacaattt taatatgcta actacacaca gaaatataaa ttttactgca gagctgtttt 28980 tcgattctac tggtaattta ctaactagac tctcatccct caaaactcca cttaatcata 29040 aatcaggaca aaacatggct actggtgcca ttactaatgc taaaggtttc atgcccagca 29100 cgactgccta tcctttcaat gataattcta gagaaaaaga aaactacatt tacggaactt 29160 gttactacac agctagtgat cgcactgctt ttcccattga catatctgtc atgcttaacc 29220 gaagagcaat aaatgacgag acatcatatt gtattcgtat aacttggtcc tggaacacag 29280 gagatgcccc agaggtgcaa acctctgcta caaccctagt cacctcccca tttacctttt 29340 actacatcag agaagacgac tgacaaataa agtttgcgat cgctaccctg caggaacttg 29400 tttatttgaa aatcaattca caaaatccga gtagttattt tgcctccccc ttcccattta 29460 acagaataca ccaatctctc cccacgcaca gctttaaaca tttggatacc attagatata 29520 gacatggttt tagattccac attccaaaca gtttcagagc gagccaatct ggggtcagtg 29580 atagataaaa atccatcggg atagtctttt aaagcgcttt cacagtccaa ctgctgcgga 29640 tgcgactccg gagtctggat cacggtcatc tggaagaaga acgatgggaa tcataatccg 29700 aaaacggtat cggacgattg tgtctcatca aacccacaag cagccgctgt ctgcgtcgct 29760 ccgtgcgact gctgtttatg ggatcagggt ccacagtgtc ctgaagcatg attttaatag 29820 cccttaacat caactttctg gtgcgatgcg cgcagcaacg cattctgatt tcactcaaat 29880 ctttgcagta ggtacaacac attattacaa tattgtttaa taaaccataa ttaaaagcgc 29940 tccagccaaa actcatatct gatataatcg cccctgcatg accatcatac caaagtttaa 30000 tataaattaa atgacgttcc ctcaaaaaca cactacccac atacatgatc tcttttggca 30060 tgtgcatatt aacaatctgt ctgtaccatg gacaacgttg gttaatcatg caacccaata 30120 taaccttccg gaaccacact gccaacaccg ctcccccagc catgcattga agtgaaccct 30180 gctgattaca atgacaatga agaacccaat tctctcgacc gtgaatcact tgagaatgaa 30240 aaatatctat agtggcacaa catagacata aatgcatgca tcttctcata atttttaact 30300 cctcaggatt tagaaacata tcccagggaa taggaagctc ttgcagaaca gtaaagctgg 30360 cagaacaagg aagaccacga acacaactta cactatgcat agtcatagta tcacaatctg 30420 gcaacagcgg gtggtcttca gtcatagaag ctcgggtttc attttcctca caacgtggta 30480 actgggctct ggtgtaaggg tgatgtctgg cgcatgatgt cgagcgtgcg cgcaaccttg 30540 tcataatgga gttgcttcct gacattctcg tattttgtat agcaaaacgc ggccctggca 30600 gaacacactc ttcttcgcct tctatcctgc cgcttagcgt gttccgtgtg atagttcaag 30660 tacaaccaca ctcttaagtt ggtcaaaaga atgctggctt cagttgtaat caaaactcca 30720 tcgcatctaa tcgttctgag gaaatcatcc aagcaatgca actggattgt gtttcaagca 30780 ggagaggaga gggaagagac ggaagaacca tgttaatttt tattccaaac gatctcgcag 30840 tacttcaaat tgtagatcgc gcagatggca tctctcgccc ccactgtgtt ggtgaaaaag 30900 cacagctaga tcaaaagaaa tgcgattttc aaggtgctca acggtggctt ccagcaaagc 30960 ctccacgcgc acatccaaga acaaaagaat accaaaagaa ggagcatttt ctaactcctc 31020 aatcatcata ttacattcct gcaccattcc cagataattt tcagctttcc agccttgaat 31080 tattcgtgtc agttcttgtg gtaaatccaa tccacacatt acaaacaggt cccggagggc 31140 gccctccacc accattctta aacacaccct cataatgaca aaatatcttg ctcctgtgtc 31200 acctgtagcg aattgagaat ggcaacatca attgacatgc ccttggctct aagttcttct 31260 ttaagttcta gttgtaaaaa ctctctcata ttatcaccaa actgcttagc cagaagcccc 31320 ccgggaacaa gagcagggga cgctacagtg cagtacaagc gcagacctcc ccaattggct 31380 ccagcaaaaa caagattgga ataagcatat tgggaaccgc cagtaatatc atcgaagttg 31440 ctggaaatat aatcaggcag agtttcttgt aaaaattgaa taaaagaaaa atttgccaaa 31500 aaaacattca aaacctctgg gatgcaaatg caataggtta ccgcgctgcg ctccaacatt 31560 gttagttttg aattagtctg caaaaataaa aaaaaaaaca agcgtcatat catagtagcc 31620 tgacgaacag atggataaat cagtctttcc atcacaagac aagccacagg gtctccagct 31680 cgaccctcgt aaaacctgtc atcatgatta aacaacagca ccgaaagttc ctcgcggtga 31740 ccagcatgaa taattcttga tgaagcatac aatccagaca tgttagcatc agttaacgag 31800 aaaaaacagc caacatagcc tttgggtata attatgctta atcgtaagta tagcaaagcc 31860 acccctcgcg gatacaaagt aaaaggcaca ggagaataaa aaatataatt atttctctgc 31920 tgctgttcag gcaacgtcgc ccccggtccc tctaaataca catacaaagc ctcatcagcc 31980 atggcttacc agacaaagta cagcgggcac acaaagcaca agctctaaag tgactctcca 32040 acctctccac aatatatata tacacaagcc ctaaactgac gtaatgggag taaagtgtaa 32100 aaaatcccgc caaacccaac acacaccccg aaactgcgtc accagggaaa agtacagttt 32160 cacttccgca atcccaacag gcgtaacttc ctctttctca cggtacgtga tatcccacta 32220 acttgcaacg tcattttccc acggtcgcac cgcccctttt agccgttaac cccacagcca 32280 atcaccacac gatccacact ttttaaaatc acctcattta catattggca ccattccatc 32340 tataaggtat attatataga taga 32364 <210> 64 <211> 35208 <212> DNA <213> Artificial Sequence <220> <223> pNG-33 (pColoAd2.4) DNA plasmid, consisting of a bacterial origin of replication (p15A), an antibiotic resistance gene (KanR) and the EnAd genome <400> 64 ggcgcgccta tctatataat ataccttata gatggaatgg tgccaatatg taaatgaggt 60 gattttaaaa agtgtggatc gtgtggtgat tggctgtggg gttaacggct aaaaggggcg 120 gtgcgaccgt gggaaaatga cgttttgtgg gggtggagtt tttttgcaag ttgtcgcggg 180 aaatgtgacg cataaaaagg cttttttctc acggaactac ttagttttcc cacggtattt 240 aacaggaaat gaggtagttt tgaccggatg caagtgaaaa ttgttgattt tcgcgcgaaa 300 actgaatgag gaagtgtttt tctgaataat gtggtattta tggcagggtg gagtatttgt 360 tcagggccag gtagactttg acccattacg tggaggtttc gattaccgtg ttttttacct 420 gaatttccgc gtaccgtgtc aaagtcttct gtttttacgt aggtgtcagc tgatcgctag 480 ggtatttata cctcagggtt tgtgtcaaga ggccactctt gagtgccagc gagaagagtt 540 ttctcctctg cgccggcagt ttaataataa aaaaatgaga gatttgcgat ttctgcctca 600 ggaaataatc tctgctgaga ctggaaatga aatattggag cttgtggtgc acgccctgat 660 gggagacgat ccggagccac ctgtgcagct ttttgagcct cctacgcttc aggaactgta 720 tgatttagag gtagagggat cggaggattc taatgaggaa gctgtaaatg gcttttttac 780 cgattctatg cttttagctg ctaatgaagg gttagaatta gatccgcctt tggacacttt 840 tgatactcca ggggtaattg tggaaagcgg tacaggtgta agaaaattac ctgatttgag 900 ttccgtggac tgtgatttgc actgctatga agacgggttt cctccgagtg atgaggagga 960 ccatgaaaag gagcagtcca tgcagactgc agcgggtgag ggagtgaagg ctgccaatgt 1020 tggttttcag ttggattgcc cggagcttcc tggacatggc tgtaagtctt gtgaatttca 1080 caggaaaaat actggagtaa aggaactgtt atgttcgctt tgttatatga gaacgcactg 1140 ccactttatt tacagtaagt gtgtttaagt taaaatttaa aggaatatgc tgtttttcac 1200 atgtatattg agtgtgagtt ttgtgcttct tattataggt cctgtgtctg atgctgatga 1260 atcaccatct cctgattcta ctacctcacc tcctgagatt caagcacctg ttcctgtgga 1320 cgtgcgcaag cccattcctg tgaagcttaa gcctgggaaa cgtccagcag tggaaaaact 1380 tgaggacttg ttacagggtg gggacggacc tttggacttg agtacacgga aacgtccaag 1440 acaataagtg ttccatatcc gtgtttactt aaggtgacgt caatatttgt gtgacagtgc 1500 aatgtaataa aaatatgtta actgttcact ggtttttatt gctttttggg cggggactca 1560 ggtatataag tagaagcaga cctgtgtggt tagctcatag gagctggctt tcatccatgg 1620 aggtttgggc cattttggaa gaccttagga agactaggca actgttagag aacgcttcgg 1680 acggagtctc cggtttttgg agattctggt tcgctagtga attagctagg gtagttttta 1740 ggataaaaca ggactataaa caagaatttg aaaagttgtt ggtagattgc ccaggacttt 1800 ttgaagctct taatttgggc catcaggttc actttaaaga aaaagtttta tcagttttag 1860 acttttcaac cccaggtaga actgctgctg ctgtggcttt tcttactttt atattagata 1920 aatggatccc gcagactcat ttcagcaggg gatacgtttt ggatttcata gccacagcat 1980 tgtggagaac atggaaggtt cgcaagatga ggacaatctt aggttactgg ccagtgcagc 2040 ctttgggtgt agcgggaatc ctgaggcatc caccggtcat gccagcggtt ctggaggagg 2100 aacagcaaga ggacaacccg agagccggcc tggaccctcc agtggaggag gcggagtagc 2160 tgacttgtct cctgaactgc aacgggtgct tactggatct acgtccactg gacgggatag 2220 gggcgttaag agggagaggg catctagtgg tactgatgct agatctgagt tggctttaag 2280 tttaatgagt cgcagacgtc ctgaaaccat ttggtggcat gaggttcaga aagagggaag 2340 ggatgaagtt tctgtattgc aggagaaata ttcactggaa caggtgaaaa catgttggtt 2400 ggagcctgag gatgattggg aggtggccat taaaaattat gccaagatag ctttgaggcc 2460 tgataaacag tataagatta ctagacggat taatatccgg aatgcttgtt acatatctgg 2520 aaatggggct gaggtggtaa tagatactca agacaaggca gttattagat gctgcatgat 2580 ggatatgtgg cctggggtag tcggtatgga agcagtaact tttgtaaatg ttaagtttag 2640 gggagatggt tataatggaa tagtgtttat ggccaatacc aaacttatat tgcatggttg 2700 tagctttttt ggtttcaaca atacctgtgt agatgcctgg ggacaggtta gtgtacgggg 2760 atgtagtttc tatgcgtgtt ggattgccac agctggcaga accaagagtc aattgtctct 2820 gaagaaatgc atatttcaaa gatgtaacct gggcattctg aatgaaggcg aagcaagggt 2880 ccgccactgc gcttctacag atactggatg ttttattttg attaagggaa atgccagcgt 2940 aaagcataac atgatttgcg gtgcttccga tgagaggcct tatcaaatgc tcacttgtgc 3000 tggtgggcat tgtaatatgc tggctactgt gcatattgtt tcccatcaac gcaaaaaatg 3060 gcctgttttt gatcacaatg tgatgacgaa gtgtaccatg catgcaggtg ggcgtagagg 3120 aatgtttatg ccttaccagt gtaacatgaa tcatgtgaaa gtgttgttgg aaccagatgc 3180 cttttccaga atgagcctaa caggaatttt tgacatgaac atgcaaatct ggaagatcct 3240 gaggtatgat gatacgagat cgagggtacg cgcatgcgaa tgcggaggca agcatgccag 3300 gttccagccg gtgtgtgtag atgtgactga agatctcaga ccggatcatt tggttattgc 3360 ccgcactgga gcagagttcg gatccagtgg agaagaaact gactaaggtg agtattggga 3420 aaactttggg gtgggatttt cagatggaca gattgagtaa aaatttgttt tttctgtctt 3480 gcagctgtca tgagtggaaa cgcttctttt aaggggggag tcttcagccc ttatctgaca 3540 gggcgtctcc catcctgggc aggagttcgt cagaatgtta tgggatctac tgtggatgga 3600 agacccgtcc aacccgccaa ttcttcaacg ctgacctatg ctactttaag ttcttcacct 3660 ttggacgcag ctgcagctgc cgccgccgct tctgttgccg ctaacactgt gcttggaatg 3720 ggttactatg gaagcatcat ggctaattcc acttcctcta ataacccttc taccctgact 3780 caggacaagt tacttgtcct tttggcccag ctggaggctt tgacccaacg tctgggtgaa 3840 ctttctcagc aggtggtcga gttgcgagta caaactgagt ctgctgtcgg cacggcaaag 3900 tctaaataaa aaaatcccag aatcaatgaa taaataaaca agcttgttgt tgatttaaaa 3960 tcaagtgttt ttatttcatt tttcgcgcac ggtatgccct agaccaccga tctctatcat 4020 tgagaactcg gtggattttt tccaggatcc tatagaggtg ggattgaatg tttagataca 4080 tgggcattag gccgtctttg gggtggagat agctccattg aagggattca tgctccgggg 4140 tagtgttgta aatcacccag tcataacaag gtcgcagtgc atggtgttgc acaatatctt 4200 ttagaagtag gctgattgcc acagataagc ccttggtgta ggtgtttaca aaccggttga 4260 gctgggatgg gtgcattcgg ggtgaaatta tgtgcatttt ggattggatt tttaagttgg 4320 caatattgcc gccaagatcc cgtcttgggt tcatgttatg aaggaccacc aagacggtgt 4380 atccggtaca tttaggaaat ttatcgtgca gcttggatgg aaaagcgtgg aaaaatttgg 4440 agacaccctt gtgtcctcca agattttcca tgcactcatc catgataata gcaatggggc 4500 cgtgggcagc ggcgcgggca aacacgttcc gtgggtctga cacatcatag ttatgttcct 4560 gagttaaatc atcataagcc attttaatga atttggggcg gagagtacca gattggggta 4620 tgaatgttcc ttcgggcccc ggagcatagt tcccctcaca gatttgcatt tcccaagctt 4680 tcagttccga gggtggaatc atgtccacct ggggggctat gaaaaacacc gtttctgggg 4740 cgggggtgat taattgtgat gatagcaaat ttctgagcaa ttgagatttg ccacatccgg 4800 tggggccata aatgattccg attacgggtt gcaggtggta gtttagggaa cggcaactgc 4860 cgtcttctcg aagcaagggg gccacctcgt tcatcatttc ccttacatgc atattttccc 4920 gcaccaaatc cattaggagg cgctctcctc ctagtgatag aagttcttgt agtgaggaaa 4980 agtttttcag cggtttcaga ccgtcagcca tgggcatttt ggagagagtt tgctgcaaaa 5040 gttctagtct gttccacagt tcagtgatgt gttctatggc atctcgatcc agcagacctc 5100 ctcgtttcgc gggtttggac ggctcctgga atagggtatg agacgatggg cgtccagcgc 5160 tgccagggtt cggtccttcc agggtctcag tgttcgagtc agggttgttt ccgtcacagt 5220 gaaggggtgt gcgcctgctt gggcgcttgc cagggtgcgc ttcagactca tcctgctggt 5280 cgaaaacttc tgtcgcttgg cgccctgtat gtcggccaag tagcagttta ccatgagttc 5340 gtagttgagc gcctcggctg cgtggccttt ggcgcggagc ttacctttgg aagttttctt 5400 gcataccggg cagtataggc atttcagcgc atacaacttg ggcgcaagga aaacggattc 5460 tggggagtat gcatctgcgc cgcaggaggc gcaaacagtt tcacattcca ccagccaggt 5520 taaatccggt tcattggggt caaaaacaag ttttccgcca tattttttga tgcgtttctt 5580 acctttggtc tccatgagtt cgtgtcctcg ttgagtgaca aacaggctgt ccgtgtcccc 5640 gtagactgat tttacaggcc tcttctccag tggagtgcct cggtcttctt cgtacaggaa 5700 ctctgaccac tctgatacaa aggcgcgcgt ccaggccagc acaaaggagg ctatgtggga 5760 ggggtagcga tcgttgtcaa ccagggggtc caccttttcc aaagtatgca aacacatgtc 5820 accctcttca acatccagga atgtgattgg cttgtaggtg tatttcacgt gacctggggt 5880 ccccgctggg ggggtataaa agggggcggt tctttgctct tcctcactgt cttccggatc 5940 gctgtccagg aacgtcagct gttggggtag gtattccctc tcgaaggcgg gcatgacctc 6000 tgcactcagg ttgtcagttt ctaagaacga ggaggatttg atattgacag tgccggttga 6060 gatgcctttc atgaggtttt cgtccatctg gtcagaaaac acaatttttt tattgtcaag 6120 tttggtggca aatgatccat acagggcgtt ggataaaagt ttggcaatgg atcgcatggt 6180 ttggttcttt tccttgtccg cgcgctcttt ggcggcgatg ttgagttgga catactcgcg 6240 tgccaggcac ttccattcgg ggaagatagt tgttaattca tctggcacga ttctcacttg 6300 ccaccctcga ttatgcaagg taattaaatc cacactggtg gccacctcgc ctcgaagggg 6360 ttcattggtc caacagagcc tacctccttt cctagaacag aaagggggaa gtgggtctag 6420 cataagttca tcgggagggt ctgcatccat ggtaaagatt cccggaagta aatccttatc 6480 aaaatagctg atgggagtgg ggtcatctaa ggccatttgc cattctcgag ctgccagtgc 6540 gcgctcatat gggttaaggg gactgcccca tggcatggga tgggtgagtg cagaggcata 6600 catgccacag atgtcataga cgtagatggg atcctcaaag atgcctatgt aggttggata 6660 gcatcgcccc cctctgatac ttgctcgcac atagtcatat agttcatgtg atggcgctag 6720 cagccccgga cccaagttgg tgcgattggg tttttctgtt ctgtagacga tctggcgaaa 6780 gatggcgtga gaattggaag agatggtggg tctttgaaaa atgttgaaat gggcatgagg 6840 tagacctaca gagtctctga caaagtgggc ataagattct tgaagcttgg ttaccagttc 6900 ggcggtgaca agtacgtcta gggcgcagta gtcaagtgtt tcttgaatga tgtcataacc 6960 tggttggttt ttcttttccc acagttcgcg gttgagaagg tattcttcgc gatccttcca 7020 gtactcttct agcggaaacc cgtctttgtc tgcacggtaa gatcctagca tgtagaactg 7080 attaactgcc ttgtaagggc agcagccctt ctctacgggt agagagtatg cttgagcagc 7140 ttttcgtagc gaagcgtgag taagggcaaa ggtgtctctg accatgactt tgaggaattg 7200 gtatttgaag tcgatgtcgt cacaggctcc ctgttcccag agttggaagt ctacccgttt 7260 cttgtaggcg gggttgggca aagcgaaagt aacatcattg aagagaatct tgccggccct 7320 gggcatgaaa ttgcgagtga tgcgaaaagg ctgtggtact tccgctcggt tattgataac 7380 ctgggcagct aggacgatct cgtcgaaacc gttgatgttg tgtcctacga tgtataattc 7440 tatgaaacgc ggcgtgcctc tgacgtgagg tagcttactg agctcatcaa aggttaggtc 7500 tgtggggtca gataaggcgt agtgttcgag agcccattcg tgcaggtgag gattcgcttt 7560 aaggaaggag gaccagaggt ccactgccag tgctgtttgt aactggtccc ggtactgacg 7620 aaaatgccgt ccgactgcca ttttttctgg ggtgacgcaa tagaaggttt gggggtcctg 7680 ccgccagcga tcccacttga gttttatggc gaggtcatag gcgatgttga cgagccgctg 7740 gtctccagag agtttcatga ccagcatgaa ggggattagc tgcttgccaa aggaccccat 7800 ccaggtgtag gtttccacat cgtaggtgag aaagagcctt tctgtgcgag gatgagagcc 7860 aatcgggaag aactggatct cctgccacca gttggaggaa tggctgttga tgtgatggaa 7920 gtagaactcc ctgcgacgcg ccgagcattc atgcttgtgc ttgtacagac ggccgcagta 7980 gtcgcagcgt tgcacgggtt gtatctcgtg aatgagttgt acctggcttc ccttgacgag 8040 aaatttcagt gggaagccga ggcctggcga ttgtatctcg tgctttacta tgttgtctgc 8100 atcggcctgt tcatcttctg tctcgatggt ggtcatgctg acgagccctc gcgggaggca 8160 agtccagacc tcggcgcggc aggggcggag ctcgaggacg agagcgcgca ggctggagct 8220 gtccagggtc ctgagacgct gcggactcag gttagtaggc agtgtcagga gattaacttg 8280 catgatcttt tggagggcgt gcgggaggtt cagatagtac ttgatctcaa cgggtccgtt 8340 ggtggagatg tcgatggctt gcagggttcc gtgtcccttg ggcgctacca ccgtgccctt 8400 gtttttcatt ttggacggcg gtggctctgt tgcttcttgc atgtttagaa gcggtgtcga 8460 gggcgcgcac cgggcggcag gggcggctcg ggacccggcg gcatggctgg cagtggtacg 8520 tcggcgccgc gcgcgggtag gttctggtac tgcgccctga gaagactcgc atgcgcgacg 8580 acgcggcggt tgacatcctg gatctgacgc ctctgggtga aagctaccgg ccccgtgagc 8640 ttgaacctga aagagagttc aacagaatca atctcggtat cgttgacggc ggcttgccta 8700 aggatttctt gcacgtcacc agagttgtcc tggtaggcga tctccgccat gaactgctcg 8760 atctcttcct cttgaagatc tccgcggccc gctctctcga cggtggccgc gaggtcgttg 8820 gagatgcgcc caatgagttg agagaatgca ttcatgcccg cctcgttcca gacgcggctg 8880 tagaccacgg cccccacggg atctctcgcg cgcatgacca cctgggcgag gttgagctcc 8940 acgtggcggg tgaagaccgc atagttgcat aggcgctgga aaaggtagtt gagtgtggtg 9000 gcgatgtgct cggtgacgaa gaaatacatg atccatcgtc tcagcggcat ctcgctgaca 9060 tcgcccagag cttccaagcg ctccatggcc tcgtagaagt ccacggcaaa attaaaaaac 9120 tgggagtttc gcgcggacac ggtcaactcc tcttccagaa gacggataag ttcggcgatg 9180 gtggtgcgca cctcgcgctc gaaagcccct gggatttctt cctcaatctc ttcttcttcc 9240 actaacatct cttcctcttc aggtggggct gcaggaggag ggggaacgcg gcgacgccgg 9300 cggcgcacgg gcagacggtc gatgaatctt tcaatgacct ctccgcggcg gcggcgcatg 9360 gtttcagtga cggcgcggcc gttctcgcgc ggtcgcagag taaaaacacc gccgcgcatc 9420 tccttaaagt ggtgactggg aggttctccg tttgggaggg agagggcgct gattatacat 9480 tttattaatt ggcccgtagg gactgcacgc agagatctga tcgtgtcaag atccacggga 9540 tctgaaaacc tttcgacgaa agcgtctaac cagtcacagt cacaaggtag gctgagtacg 9600 gcttcttgtg ggcgggggtg gttatgtgtt cggtctgggt cttctgtttc ttcttcatct 9660 cgggaaggtg agacgatgct gctggtgatg aaattaaagt aggcagttct aagacggcgg 9720 atggtggcga ggagcaccag gtctttgggt ccggcttgct ggatacgcag gcgattggcc 9780 attccccaag cattatcctg acatctagca agatctttgt agtagtcttg catgagccgt 9840 tctacgggca cttcttcctc acccgttctg ccatgcatac gtgtgagtcc aaatccgcgc 9900 attggttgta ccagtgccaa gtcagctacg actctttcgg cgaggatggc ttgctgtact 9960 tgggtaaggg tggcttgaaa gtcatcaaaa tccacaaagc ggtggtaagc tcctgtatta 10020 atggtgtaag cacagttggc catgactgac cagttaactg tctggtgacc agggcgcacg 10080 agctcggtgt atttaaggcg cgaataggcg cgggtgtcaa agatgtaatc gttgcaggtg 10140 cgcaccagat actggtaccc tataagaaaa tgcggcggtg gttggcggta gagaggccat 10200 cgttctgtag ctggagcgcc aggggcgagg tcttccaaca taaggcggtg atagccgtag 10260 atgtacctgg acatccaggt gattcctgcg gcggtagtag aagcccgagg aaactcgcgt 10320 acgcggttcc aaatgttgcg tagcggcatg aagtagttca ttgtaggcac ggtttgacca 10380 gtgaggcgcg cgcagtcatt gatgctctat agacacggag aaaatgaaag cgttcagcga 10440 ctcgactccg tagcctggag gaacgtgaac gggttgggtc gcggtgtacc ccggttcgag 10500 acttgtactc gagccggccg gagccgcggc taacgtggta ttggcactcc cgtctcgacc 10560 cagcctacaa aaatccagga tacggaatcg agtcgttttg ctggtttccg aatggcaggg 10620 aagtgagtcc tatttttttt ttttgccgct cagatgcatc ccgtgctgcg acagatgcgc 10680 ccccaacaac agcccccctc gcagcagcag cagcagcaat cacaaaaggc tgtccctgca 10740 actactgcaa ctgccgccgt gagcggtgcg ggacagcccg cctatgatct ggacttggaa 10800 gagggcgaag gactggcacg tctaggtgcg ccttcacccg agcggcatcc gcgagttcaa 10860 ctgaaaaaag attctcgcga ggcgtatgtg ccccaacaga acctatttag agacagaagc 10920 ggcgaggagc cggaggagat gcgagcttcc cgctttaacg cgggtcgtga gctgcgtcac 10980 ggtttggacc gaagacgagt gttgcgggac gaggatttcg aagttgatga aatgacaggg 11040 atcagtcctg ccagggcaca cgtggctgca gccaaccttg tatcggctta cgagcagaca 11100 gtaaaggaag agcgtaactt ccaaaagtct tttaataatc atgtgcgaac cctgattgcc 11160 cgcgaagaag ttacccttgg tttgatgcat ttgtgggatt tgatggaagc tatcattcag 11220 aaccctacta gcaaacctct gaccgcccag ctgtttctgg tggtgcaaca cagcagagac 11280 aatgaggctt tcagagaggc gctgctgaac atcaccgaac ccgaggggag atggttgtat 11340 gatcttatca acattctaca gagtatcata gtgcaggagc ggagcctggg cctggccgag 11400 aaggtggctg ccatcaatta ctcggttttg agcttgggaa aatattacgc tcgcaaaatc 11460 tacaagactc catacgttcc catagacaag gaggtgaaga tagatgggtt ctacatgcgc 11520 atgacgctca aggtcttgac cctgagcgat gatcttgggg tgtatcgcaa tgacagaatg 11580 catcgcgcgg ttagcgccag caggaggcgc gagttaagcg acagggaact gatgcacagt 11640 ttgcaaagag ctctgactgg agctggaacc gagggtgaga attacttcga catgggagct 11700 gacttgcagt ggcagcctag tcgcagggct ctgagcgccg cgacggcagg atgtgagctt 11760 ccttacatag aagaggcgga tgaaggcgag gaggaagagg gcgagtactt ggaagactga 11820 tggcacaacc cgtgtttttt gctagatgga acagcaagca ccggatcccg caatgcgggc 11880 ggcgctgcag agccagccgt ccggcattaa ctcctcggac gattggaccc aggccatgca 11940 acgtatcatg gcgttgacga ctcgcaaccc cgaagccttt agacagcaac cccaggccaa 12000 ccgtctatcg gccatcatgg aagctgtagt gccttcccgc tctaatccca ctcatgagaa 12060 ggtcctggcc atcgtgaacg cgttggtgga gaacaaagct attcgtccag atgaggccgg 12120 actggtatac aacgctctct tagaacgcgt ggctcgctac aacagtagca atgtgcaaac 12180 caatttggac cgtatgataa cagatgtacg cgaagccgtg tctcagcgcg aaaggttcca 12240 gcgtgatgcc aacctgggtt cgctggtggc gttaaatgct ttcttgagta ctcagcctgc 12300 taatgtgccg cgtggtcaac aggattatac taacttttta agtgctttga gactgatggt 12360 atcagaagta cctcagagcg aagtgtatca gtccggtcct gattacttct ttcagactag 12420 cagacagggc ttgcagacgg taaatctgag ccaagctttt aaaaacctta aaggtttgtg 12480 gggagtgcat gccccggtag gagaaagagc aaccgtgtct agcttgttaa ctccgaactc 12540 ccgcctatta ttactgttgg tagctccttt caccgacagc ggtagcatcg accgtaattc 12600 ctatttgggt tacctactaa acctgtatcg cgaagccata gggcaaagtc aggtggacga 12660 gcagacctat caagaaatta cccaagtcag tcgcgctttg ggacaggaag acactggcag 12720 tttggaagcc actctgaact tcttgcttac caatcggtct caaaagatcc ctcctcaata 12780 tgctcttact gcggaggagg agaggatcct tagatatgtg cagcagagcg tgggattgtt 12840 tctgatgcaa gagggggcaa ctccgactgc agcactggac atgacagcgc gaaatatgga 12900 gcccagcatg tatgccagta accgaccttt cattaacaaa ctgctggact acttgcacag 12960 agctgccgct atgaactctg attatttcac caatgccatc ttaaacccgc actggctgcc 13020 cccacctggt ttctacacgg gcgaatatga catgcccgac cctaatgacg gatttctgtg 13080 ggacgacgtg gacagcgatg ttttttcacc tctttctgat catcgcacgt ggaaaaagga 13140 aggcggcgat agaatgcatt cttctgcatc gctgtccggg gtcatgggtg ctaccgcggc 13200 tgagcccgag tctgcaagtc cttttcctag tctacccttt tctctacaca gtgtacgtag 13260 cagcgaagtg ggtagaataa gtcgcccgag tttaatgggc gaagaggagt atctaaacga 13320 ttccttgctc agaccggcaa gagaaaaaaa tttcccaaac aatggaatag aaagtttggt 13380 ggataaaatg agtagatgga agacttatgc tcaggatcac agagacgagc ctgggatcat 13440 ggggattaca agtagagcga gccgtagacg ccagcgccat gacagacaga ggggtcttgt 13500 gtgggacgat gaggattcgg ccgatgatag cagcgtgctg gacttgggtg ggagaggaag 13560 gggcaacccg tttgctcatt tgcgccctcg cttgggtggt atgttgtaaa aaaaaataaa 13620 aaaaaaactc accaaggcca tggcgacgag cgtacgttcg ttcttcttta ttatctgtgt 13680 ctagtataat gaggcgagtc gtgctaggcg gagcggtggt gtatccggag ggtcctcctc 13740 cttcgtacga gagcgtgatg cagcagcagc aggcgacggc ggtgatgcaa tccccactgg 13800 aggctccctt tgtgcctccg cgatacctgg cacctacgga gggcagaaac agcattcgtt 13860 attcggaact ggcacctcag tacgatacca ccaggttgta tctggtggac aacaagtcgg 13920 cggacattgc ttctctgaac tatcagaatg accacagcaa cttcttgacc acggtggtgc 13980 aaaacaatga ctttacccct acggaagcca gcacccagac cattaacttt gatgaacgat 14040 cgcggtgggg cggtcagcta aagaccatca tgcatactaa catgccaaac gtgaacgagt 14100 atatgtttag taacaagttc aaagcgcgtg tgatggtgtc cagaaaacct cccgacggtg 14160 ctgcagttgg ggatacttat gatcacaagc aggatatttt gaaatatgag tggttcgagt 14220 ttactttgcc agaaggcaac ttttcagtta ctatgactat tgatttgatg aacaatgcca 14280 tcatagataa ttacttgaaa gtgggtagac agaatggagt gcttgaaagt gacattggtg 14340 ttaagttcga caccaggaac ttcaagctgg gatgggatcc cgaaaccaag ttgatcatgc 14400 ctggagtgta tacgtatgaa gccttccatc ctgacattgt cttactgcct ggctgcggag 14460 tggattttac cgagagtcgt ttgagcaacc ttcttggtat cagaaaaaaa cagccatttc 14520 aagagggttt taagattttg tatgaagatt tagaaggtgg taatattccg gccctcttgg 14580 atgtagatgc ctatgagaac agtaagaaag aacaaaaagc caaaatagaa gctgctacag 14640 ctgctgcaga agctaaggca aacatagttg ccagcgactc tacaagggtt gctaacgctg 14700 gagaggtcag aggagacaat tttgcgccaa cacctgttcc gactgcagaa tcattattgg 14760 ccgatgtgtc tgaaggaacg gacgtgaaac tcactattca acctgtagaa aaagatagta 14820 agaatagaag ctataatgtg ttggaagaca aaatcaacac agcctatcgc agttggtatc 14880 tttcgtacaa ttatggcgat cccgaaaaag gagtgcgttc ctggacattg ctcaccacct 14940 cagatgtcac ctgcggagca gagcaggtct actggtcgct tccagacatg atgaaggatc 15000 ctgtcacttt ccgctccact agacaagtca gtaactaccc tgtggtgggt gcagagctta 15060 tgcccgtctt ctcaaagagc ttctacaacg aacaagctgt gtactcccag cagctccgcc 15120 agtccacctc gcttacgcac gtcttcaacc gctttcctga gaaccagatt ttaatccgtc 15180 cgccggcgcc caccattacc accgtcagtg aaaacgttcc tgctctcaca gatcacggga 15240 ccctgccgtt gcgcagcagt atccggggag tccaacgtgt gaccgttact gacgccagac 15300 gccgcacctg tccctacgtg tacaaggcac tgggcatagt cgcaccgcgc gtcctttcaa 15360 gccgcacttt ctaaaaaaaa aaaaaatgtc cattcttatc tcgcccagta ataacaccgg 15420 ttggggtctg cgcgctccaa gcaagatgta cggaggcgca cgcaaacgtt ctacccaaca 15480 tcctgtccgt gttcgcggac attttcgcgc tccatggggc gccctcaagg gccgcactcg 15540 cgttcgaacc accgtcgatg atgtaatcga tcaggtggtt gccgacgccc gtaattatac 15600 tcctactgcg cctacatcta ctgtggatgc agttattgac agtgtagtgg ctgacgctcg 15660 caactatgct cgacgtaaga gccggcgaag gcgcattgcc agacgccacc gagctaccac 15720 tgccatgcga gccgcaagag ctctgctacg aagagctaga cgcgtggggc gaagagccat 15780 gcttagggcg gccagacgtg cagcttcggg cgccagcgcc ggcaggtccc gcaggcaagc 15840 agccgctgtc gcagcggcga ctattgccga catggcccaa tcgcgaagag gcaatgtata 15900 ctgggtgcgt gacgctgcca ccggtcaacg tgtacccgtg cgcacccgtc cccctcgcac 15960 ttagaagata ctgagcagtc tccgatgttg tgtcccagcg gcgaggatgt ccaagcgcaa 16020 atacaaggaa gaaatgctgc aggttatcgc acctgaagtc tacggccaac cgttgaagga 16080 tgaaaaaaaa ccccgcaaaa tcaagcgggt taaaaaggac aaaaaagaag aggaagatgg 16140 cgatgatggg ctggcggagt ttgtgcgcga gtttgcccca cggcgacgcg tgcaatggcg 16200 tgggcgcaaa gttcgacatg tgttgagacc tggaacttcg gtggtcttta cacccggcga 16260 gcgttcaagc gctactttta agcgttccta tgatgaggtg tacggggatg atgatattct 16320 tgagcaggcg gctgaccgat taggcgagtt tgcttatggc aagcgtagta gaataacttc 16380 caaggatgag acagtgtcga tacccttgga tcatggaaat cccaccccta gtcttaaacc 16440 ggtcactttg cagcaagtgt tacccgtaac tccgcgaaca ggtgttaaac gcgaaggtga 16500 agatttgtat cccactatgc aactgatggt acccaaacgc cagaagttgg aggacgtttt 16560 ggagaaagta aaagtggatc cagatattca acctgaggtt aaagtgagac ccattaagca 16620 ggtagcgcct ggtctggggg tacaaactgt agacattaag attcccactg aaagtatgga 16680 agtgcaaact gaacccgcaa agcctactgc cacctccact gaagtgcaaa cggatccatg 16740 gatgcccatg cctattacaa ctgacgccgc cggtcccact cgaagatccc gacgaaagta 16800 cggtccagca agtctgttga tgcccaatta tgttgtacac ccatctatta ttcctactcc 16860 tggttaccga ggcactcgct actatcgcag ccgaaacagt acctcccgcc gtcgccgcaa 16920 gacacctgca aatcgcagtc gtcgccgtag acgcacaagc aaaccgactc ccggcgccct 16980 ggtgcggcaa gtgtaccgca atggtagtgc ggaacctttg acactgccgc gtgcgcgtta 17040 ccatccgagt atcatcactt aatcaatgtt gccgctgcct ccttgcagat atggccctca 17100 cttgtcgcct tcgcgttccc atcactggtt accgaggaag aaactcgcgc cgtagaagag 17160 ggatgttggg acgcggaatg cgacgctaca ggcgacggcg tgctatccgc aagcaattgc 17220 ggggtggttt tttaccagcc ttaattccaa ttatcgctgc tgcaattggc gcgataccag 17280 gcatagcttc cgtggcggtt caggcctcgc aacgacattg acattggaaa aaaacgtata 17340 aataaaaaaa aaaaaataca atggactctg acactcctgg tcctgtgact atgttttctt 17400 agagatggaa gacatcaatt tttcatcctt ggctccgcga cacggcacga agccgtacat 17460 gggcacctgg agcgacatcg gcacgagcca actgaacggg ggcgccttca attggagcag 17520 tatctggagc gggcttaaaa attttggctc aaccataaaa acatacggga acaaagcttg 17580 gaacagcagt acaggacagg cgcttagaaa taaacttaaa gaccagaact tccaacaaaa 17640 agtagtcgat gggatagctt ccggcatcaa tggagtggta gatttggcta accaggctgt 17700 gcagaaaaag ataaacagtc gtttggaccc gccgccagca accccaggtg aaatgcaagt 17760 ggaggaagaa attcctccgc cagaaaaacg aggcgacaag cgtccgcgtc ccgatttgga 17820 agagacgctg gtgacgcgcg tagatgaacc gccttcttat gaggaagcaa cgaagcttgg 17880 aatgcccacc actagaccga tagccccaat ggccaccggg gtgatgaaac cttctcagtt 17940 gcatcgaccc gtcaccttgg atttgccccc tccccctgct gctactgctg tacccgcttc 18000 taagcctgtc gctgccccga aaccagtcgc cgtagccagg tcacgtcccg ggggcgctcc 18060 tcgtccaaat gcgcactggc aaaatactct gaacagcatc gtgggtctag gcgtgcaaag 18120 tgtaaaacgc cgtcgctgct tttaattaaa tatggagtag cgcttaactt gcctatctgt 18180 gtatatgtgt cattacacgc cgtcacagca gcagaggaaa aaaggaagag gtcgtgcgtc 18240 gacgctgagt tactttcaag atggccaccc catcgatgct gccccaatgg gcatacatgc 18300 acatcgccgg acaggatgct tcggagtacc tgagtccggg tctggtgcag ttcgcccgcg 18360 ccacagacac ctacttcaat ctgggaaata agtttagaaa tcccaccgta gcgccgaccc 18420 acgatgtgac caccgaccgt agccagcggc tcatgttgcg cttcgtgccc gttgaccggg 18480 aggacaatac atactcttac aaagtgcggt acaccctggc cgtgggcgac aacagagtgc 18540 tggatatggc cagcacgttc tttgacatta ggggtgtgtt ggacagaggt cccagtttca 18600 aaccctattc tggtacggct tacaactccc tggctcctaa aggcgctcca aatacatctc 18660 agtggattgc agaaggtgta aaaaatacaa ctggtgagga acacgtaaca gaagaggaaa 18720 ccaatactac tacttacact tttggcaatg ctcctgtaaa agctgaagct gaaattacaa 18780 aagaaggact cccagtaggt ttggaagttt cagatgaaga aagtaaaccg atttatgctg 18840 ataaaacata tcagccagaa cctcagctgg gagatgaaac ttggactgac cttgatggaa 18900 aaaccgaaaa gtatggaggc agggctctca aacccgatac taagatgaaa ccatgctacg 18960 ggtcctttgc caaacctact aatgtgaaag gcggtcaggc aaaacaaaaa acaacggagc 19020 agccaaatca gaaagtcgaa tatgatatcg acatggagtt ttttgatgcg gcatcgcaga 19080 aaacaaactt aagtcctaaa attgtcatgt atgcagaaaa tgtaaatttg gaaactccag 19140 acactcatgt agtgtacaaa cctggaacag aagacacaag ttccgaagct aatttgggac 19200 aacaatctat gcccaacaga cccaactaca ttggcttcag agataacttt attggactta 19260 tgtactataa cagtactggt aacatggggg tgctggctgg tcaagcgtct cagttaaatg 19320 cagtggttga cttgcaggac agaaacacag aactttctta ccaactcttg cttgactctc 19380 tgggcgacag aaccagatac tttagcatgt ggaatcaggc tgtggacagt tatgatcctg 19440 atgtacgtgt tattgaaaat catggtgtgg aagatgaact tcccaactac tgttttccac 19500 tggacggcat aggtgttcca acaaccagtt acaaatcaat agttccaaat ggagacaatg 19560 cgcctaattg gaaggaacct gaagtaaatg gaacaagtga gatcggacag ggtaatttgt 19620 ttgccatgga aattaacctt caagccaatc tatggcgaag tttcctttat tccaatgtgg 19680 ctctatatct cccagactcg tacaaataca ccccgtccaa tgtcactctt ccagaaaaca 19740 aaaacaccta cgactacatg aacgggcggg tggtgccgcc atctctagta gacacctatg 19800 tgaacattgg tgccaggtgg tctctggatg ccatggacaa tgtcaaccca ttcaaccacc 19860 accgtaacgc tggcttgcgt taccgatcca tgcttctggg taacggacgt tatgtgcctt 19920 tccacataca agtgcctcaa aaattcttcg ctgttaaaaa cctgctgctt ctcccaggct 19980 cctacactta tgagtggaac tttaggaagg atgtgaacat ggttctacag agttccctcg 20040 gtaacgacct gcgggtagat ggcgccagca tcagtttcac gagcatcaac ctctatgcta 20100 cttttttccc catggctcac aacaccgctt ccacccttga agccatgctg cggaatgaca 20160 ccaatgatca gtcattcaac gactacctat ctgcagctaa catgctctac cccattcctg 20220 ccaatgcaac caatattccc atttccattc cttctcgcaa ctgggcggct ttcagaggct 20280 ggtcatttac cagactgaaa accaaagaaa ctccctcttt ggggtctgga tttgacccct 20340 actttgtcta ttctggttct attccctacc tggatggtac cttctacctg aaccacactt 20400 ttaagaaggt ttccatcatg tttgactctt cagtgagctg gcctggaaat gacaggttac 20460 tatctcctaa cgaatttgaa ataaagcgca ctgtggatgg cgaaggctac aacgtagccc 20520 aatgcaacat gaccaaagac tggttcttgg tacagatgct cgccaactac aacatcggct 20580 atcagggctt ctacattcca gaaggataca aagatcgcat gtattcattt ttcagaaact 20640 tccagcccat gagcaggcag gtggttgatg aggtcaatta caaagacttc aaggccgtcg 20700 ccatacccta ccaacacaac aactctggct ttgtgggtta catggctccg accatgcgcc 20760 aaggtcaacc ctatcccgct aactatccct atccactcat tggaacaact gccgtaaata 20820 gtgttacgca gaaaaagttc ttgtgtgaca gaaccatgtg gcgcataccg ttctcgagca 20880 acttcatgtc tatgggggcc cttacagact tgggacagaa tatgctctat gccaactcag 20940 ctcatgctct ggacatgacc tttgaggtgg atcccatgga tgagcccacc ctgctttatc 21000 ttctcttcga agttttcgac gtggtcagag tgcatcagcc acaccgcggc atcatcgagg 21060 cagtctacct gcgtacaccg ttctcggccg gtaacgctac cacgtaagaa gcttcttgct 21120 tcttgcaaat agcagctgca accatggcct gcggatccca aaacggctcc agcgagcaag 21180 agctcagagc cattgtccaa gacctgggtt gcggacccta ttttttggga acctacgata 21240 agcgcttccc ggggttcatg gcccccgata agctcgcctg tgccattgta aatacggccg 21300 gacgtgagac ggggggagag cactggttgg ctttcggttg gaacccacgt tctaacacct 21360 gctacctttt tgatcctttt ggattctcgg atgatcgtct caaacagatt taccagtttg 21420 aatatgaggg tctcctgcgc cgcagcgctc ttgctaccaa ggaccgctgt attacgctgg 21480 aaaaatctac ccagaccgtg cagggtcccc gttctgccgc ctgcggactt ttctgctgca 21540 tgttccttca cgcctttgtg cactggcctg accgtcccat ggacggaaac cccaccatga 21600 aattgctaac tggagtgcca aacaacatgc ttcattctcc taaagtccag cccaccctgt 21660 gtgacaatca aaaagcactc taccattttc ttaataccca ttcgccttat tttcgctccc 21720 atcgtacaca catcgaaagg gccactgcgt tcgaccgtat ggatgttcaa taatgactca 21780 tgtaaacaac gtgttcaata aacatcactt tattttttta catgtatcaa ggctctgcat 21840 tacttattta tttacaagtc gaatgggttc tgacgagaat cagaatgacc cgcaggcagt 21900 gatacgttgc ggaactgata cttgggttgc cacttgaatt cgggaatcac caacttggga 21960 accggtatat cgggcaggat gtcactccac agctttctgg tcagctgcaa agctccaagc 22020 aggtcaggag ccgaaatctt gaaatcacaa ttaggaccag tgctttgagc gcgagagttg 22080 cggtacaccg gattgcagca ctgaaacacc atcagcgacg gatgtctcac gcttgccagc 22140 acggtgggat ctgcaatcat gcccacatcc agatcttcag cattggcaat gctgaacggg 22200 gtcatcttgc aggtctgcct acccatggcg ggcacccaat taggcttgtg gttgcaatcg 22260 cagtgcaggg ggatcagtat catcttggcc tgatcctgtc tgattcctgg atacacggct 22320 ctcatgaaag catcatattg cttgaaagcc tgctgggctt tactaccctc ggtataaaac 22380 atcccgcagg acctgctcga aaactggtta gctgcacagc cggcatcatt cacacagcag 22440 cgggcgtcat tgttagctat ttgcaccaca cttctgcccc agcggttttg ggtgattttg 22500 gttcgctcgg gattctcctt taaggctcgt tgtccgttct cgctggccac atccatctcg 22560 ataatctgct ccttctgaat cataatattg ccatgcaggc acttcagctt gccctcataa 22620 tcattgcagc catgaggcca caacgcacag cctgtacatt cccaattatg gtgggcgatc 22680 tgagaaaaag aatgtatcat tccctgcaga aatcttccca tcatcgtgct cagtgtcttg 22740 tgactagtga aagttaactg gatgcctcgg tgctcctcgt ttacgtactg gtgacagatg 22800 cgcttgtatt gttcgtgttg ctcaggcatt agtttaaaag aggttctaag ttcgttatcc 22860 agcctgtact tctccatcag cagacacatc acttccatgc ctttctccca agcagacacc 22920 aggggcaagc taatcggatt cttaacagtg caggcagcag ctcctttagc cagagggtca 22980 tctttagcga tcttctcaat gcttcttttg ccatccttct caacgatgcg cacgggcggg 23040 tagctgaaac ccactgctac aagttgcgcc tcttctcttt cttcttcgct gtcttgactg 23100 atgtcttgca tggggatatg tttggtcttc cttggcttct ttttgggggg tatcggagga 23160 ggaggactgt cgctccgttc cggagacagg gaggattgtg acgtttcgct caccattacc 23220 aactgactgt cggtagaaga acctgacccc acacggcgac aggtgtttct cttcgggggc 23280 agaggtggag gcgattgcga agggctgcgg tccgacctgg aaggcggatg actggcagaa 23340 ccccttccgc gttcgggggt gtgctccctg tggcggtcgc ttaactgatt tccttcgcgg 23400 ctggccattg tgttctccta ggcagagaaa caacagacat ggaaactcag ccattgctgt 23460 caacatcgcc acgagtgcca tcacatctcg tcctcagcga cgaggaaaag gagcagagct 23520 taagcattcc accgcccagt cctgccacca cctctaccct agaagataag gaggtcgacg 23580 catctcatga catgcagaat aaaaaagcga aagagtctga gacagacatc gagcaagacc 23640 cgggctatgt gacaccggtg gaacacgagg aagagttgaa acgctttcta gagagagagg 23700 atgaaaactg cccaaaacaa cgagcagata actatcacca agatgctgga aatagggatc 23760 agaacaccga ctacctcata gggcttgacg gggaagacgc gctccttaaa catctagcaa 23820 gacagtcgct catagtcaag gatgcattat tggacagaac tgaagtgccc atcagtgtgg 23880 aagagctcag ccgcgcctac gagcttaacc tcttttcacc tcgtactccc cccaaacgtc 23940 agccaaacgg cacctgcgag ccaaatcctc gcttaaactt ttatccagct tttgctgtgc 24000 cagaagtact ggctacctat cacatctttt ttaaaaatca aaaaattcca gtctcctgcc 24060 gcgctaatcg cacccgcgcc gatgccctac tcaatctggg acctggttca cgcttacctg 24120 atatagcttc cttggaagag gttccaaaga tcttcgaggg tctgggcaat aatgagactc 24180 gggccgcaaa tgctctgcaa aagggagaaa atggcatgga tgagcatcac agcgttctgg 24240 tggaattgga aggcgataat gccagactcg cagtactcaa gcgaagcatc gaggtcacac 24300 acttcgcata tcccgctgtc aacctgcccc ctaaagtcat gacggcggtc atggaccagt 24360 tactcattaa gcgcgcaagt cccctttcag aagacatgca tgacccagat gcctgtgatg 24420 agggtaaacc agtggtcagt gatgagcagc taacccgatg gctgggcacc gactctccca 24480 gggatttgga agagcgtcgc aagcttatga tggccgtggt gctggttacc gtagaactag 24540 agtgtctccg acgtttcttt accgattcag aaaccttgcg caaactcgaa gagaatctgc 24600 actacacttt tagacacggc tttgtgcggc aggcatgcaa gatatctaac gtggaactca 24660 ccaacctggt ttcctacatg ggtattctgc atgagaatcg cctaggacaa agcgtgctgc 24720 acagcaccct gaagggggaa gcccgccgtg attacatccg cgattgtgtc tatctgtacc 24780 tgtgccacac gtggcaaacc ggcatgggtg tatggcagca atgtttagaa gaacagaact 24840 tgaaagagct tgacaagctc ttacagaaat ctcttaaggt tctgtggaca gggttcgacg 24900 agcgcaccgt cgcttccgac ctggcagacc tcatcttccc agagcgtctc agggttactt 24960 tgcgaaacgg attgcctgac tttatgagcc agagcatgct taacaatttt cgctctttca 25020 tcctggaacg ctccggtatc ctgcccgcca cctgctgcgc actgccctcc gactttgtgc 25080 ctctcaccta ccgcgagtgc cccccgccgc tatggagtca ctgctacctg ttccgtctgg 25140 ccaactatct ctcctaccac tcggatgtga tcgaggatgt gagcggagac ggcttgctgg 25200 agtgtcactg ccgctgcaat ctgtgcacgc cccaccggtc cctagcttgc aacccccagt 25260 tgatgagcga aacccagata ataggcacct ttgaattgca aggccccagc agccaaggcg 25320 atgggtcttc tcctgggcaa agtttaaaac tgaccccggg actgtggacc tccgcctact 25380 tgcgcaagtt tgctccggaa gattaccacc cctatgaaat caagttctat gaggaccaat 25440 cacagcctcc aaaggccgaa ctttcggcct gcgtcatcac ccagggggca attctggccc 25500 aattgcaagc catccaaaaa tcccgccaag aatttctact gaaaaagggt aagggggtct 25560 accttgaccc ccagaccggc gaggaactca acacaaggtt ccctcaggat gtcccaacga 25620 cgagaaaaca agaagttgaa ggtgcagccg ccgcccccag aagatatgga ggaagattgg 25680 gacagtcagg cagaggaggc ggaggaggac agtctggagg acagtctgga ggaagacagt 25740 ttggaggagg aaaacgagga ggcagaggag gtggaagaag taaccgccga caaacagtta 25800 tcctcggctg cggagacaag caacagcgct accatctccg ctccgagtcg aggaacccgg 25860 cggcgtccca gcagtagatg ggacgagacc ggacgcttcc cgaacccaac cagcgcttcc 25920 aagaccggta agaaggatcg gcagggatac aagtcctggc gggggcataa gaatgccatc 25980 atctcctgct tgcatgagtg cgggggcaac atatccttca cgcggcgcta cttgctattc 26040 caccatgggg tgaactttcc gcgcaatgtt ttgcattact accgtcacct ccacagcccc 26100 tactatagcc agcaaatccc ggcagtctcg acagataaag acagcggcgg cgacctccaa 26160 cagaaaacca gcagcggcag ttagaaaata cacaacaagt gcagcaacag gaggattaaa 26220 gattacagcc aacgagccag cgcaaacccg agagttaaga aatcggatct ttccaaccct 26280 gtatgccatc ttccagcaga gtcggggtca agagcaggaa ctgaaaataa aaaaccgatc 26340 tctgcgttcg ctcaccagaa gttgtttgta tcacaagagc gaagatcaac ttcagcgcac 26400 tctcgaggac gccgaggctc tcttcaacaa gtactgcgcg ctgactctta aagagtaggc 26460 agcgaccgcg cttattcaaa aaaggcggga attacatcat cctcgacatg agtaaagaaa 26520 ttcccacgcc ttacatgtgg agttatcaac cccaaatggg attggcggca ggcgcctccc 26580 aggactactc cacccgcatg aattggctca gcgccgggcc ttctatgatt tctcgagtta 26640 atgatatacg cgcctaccga aaccaaatac ttttggaaca gtcagctctt accaccacgc 26700 cccgccaaca ccttaatccc agaaattggc ccgccgccct agtgtaccag gaaagtcccg 26760 ctcccaccac tgtattactt cctcgagacg cccaggccga agtccaaatg actaatgcag 26820 gtgcgcagtt agctggcggc tccaccctat gtcgtcacag gcctcggcat aatataaaac 26880 gcctgatgat cagaggccga ggtatccagc tcaacgacga gtcggtgagc tctccgcttg 26940 gtctacgacc agacggaatc tttcagattg ccggctgcgg gagatcttcc ttcacccctc 27000 gtcaggctgt tctgactttg gaaagttcgt cttcgcaacc ccgctcgggc ggaatcggga 27060 ccgttcaatt tgtggaggag tttactccct ctgtctactt caaccccttc tccggatctc 27120 ctgggcatta cccggacgag ttcataccga acttcgacgc gattagcgag tcagtggacg 27180 gctacgattg atgtctggtg acgcggctga gctatctcgg ctgcgacatc tagaccactg 27240 ccgccgcttt cgctgctttg cccgggaact cattgagttc atctacttcg aactccccaa 27300 ggatcaccct caaggtccgg cccacggagt gcggatttct atcgaaggca aaatagactc 27360 tcgcctgcaa cgaattttct cccagcggcc cgtgctgatc gagcgagacc agggaaacac 27420 cacggtttcc atctactgca tttgtaatca ccccggattg catgaaagcc tttgctgtct 27480 tatgtgtact gagtttaata aaaactgaat taagactctc ctacggactg ccgcttcttc 27540 aacccggatt ttacaaccag aagaacgaaa cttttcctgt cgtccaggac tctgttaact 27600 tcacctttcc tactcacaaa ctagaagctc aacgactaca ccgcttttcc agaagcattt 27660 tccctactaa tactactttc aaaaccggag gtgagctcca aggtcttcct acagaaaacc 27720 cttgggtgga agcgggcctt gtagtgctag gaattcttgc gggtgggctt gtgattattc 27780 tttgctacct atacacacct tgcttcactt tcttagtggt gttgtggtat tggtttaaaa 27840 aatggggccc atactagtct tgcttgtttt actttcgctt ttggaaccgg gttctgccaa 27900 ttacgatcca tgtctagact tcgacccaga aaactgcaca cttacttttg cacccgacac 27960 aagccgcatc tgtggagttc atcgcctctc ttacgaactt ggcccccaac gacaaaaatt 28020 tacctgcatg gtgggaatca accccatagt tatcacccag caaagtggag atactaaggg 28080 ttgcattcac tgctcctgcg attccatcga gtgcacctac accctgctga agaccctatg 28140 cggcctaaga gacctgctac caatgaatta aaaaatgatt aataaaaaat cacttacttg 28200 aaatcagcaa taaggtctct gttgaaattt tctcccagca gcacctcact tccctcttcc 28260 caactctggt attctaaacc ccgttcagcg gcatactttc tccatacttt aaaggggatg 28320 tcaaatttta gctcctctcc tgtacccaca atcttcatgt ctttcttccc agatgaccaa 28380 gagagtccgg ctcagtgact ccttcaaccc tgtctacccc tatgaagatg aaagcacctc 28440 ccaacacccc tttataaacc cagggtttat ttccccaaat ggcttcacac aaagcccaaa 28500 cggagttctt actttaaaat gtttaacccc actaacaacc acaggcggat ctctacagct 28560 aaaagtggga gggggactta cagtggatga caccaacggt tttttgaaag aaaacataag 28620 tgccaccaca ccactcgtta agactggtca ctctataggt ttaccactag gagccggatt 28680 gggaacgaat gaaaataaac tttgtatcaa attaggacaa ggacttacat tcaattcaaa 28740 caacatttgc attgatgaca atattaacac cttatggaca ggagtcaacc ccaccgaagc 28800 caactgtcaa atcatgaact ccagtgaatc taatgattgc aaattaattc taacactagt 28860 taaaactgga gcactagtca ctgcatttgt ttatgttata ggagtatcta acaattttaa 28920 tatgctaact acacacagaa atataaattt tactgcagag ctgtttttcg attctactgg 28980 taatttacta actagactct catccctcaa aactccactt aatcataaat caggacaaaa 29040 catggctact ggtgccatta ctaatgctaa aggtttcatg cccagcacga ctgcctatcc 29100 tttcaatgat aattctagag aaaaagaaaa ctacatttac ggaacttgtt actacacagc 29160 tagtgatcgc actgcttttc ccattgacat atctgtcatg cttaaccgaa gagcaataaa 29220 tgacgagaca tcatattgta ttcgtataac ttggtcctgg aacacaggag atgccccaga 29280 ggtgcaaacc tctgctacaa ccctagtcac ctccccattt accttttact acatcagaga 29340 agacgactga caaataaagt ttgcgatcgc taccctgcag gaacttgttt atttgaaaat 29400 caattcacaa aatccgagta gttattttgc ctcccccttc ccatttaaca gaatacacca 29460 atctctcccc acgcacagct ttaaacattt ggataccatt agatatagac atggttttag 29520 attccacatt ccaaacagtt tcagagcgag ccaatctggg gtcagtgata gataaaaatc 29580 catcgggata gtcttttaaa gcgctttcac agtccaactg ctgcggatgc gactccggag 29640 tctggatcac ggtcatctgg aagaagaacg atgggaatca taatccgaaa acggtatcgg 29700 acgattgtgt ctcatcaaac ccacaagcag ccgctgtctg cgtcgctccg tgcgactgct 29760 gtttatggga tcagggtcca cagtgtcctg aagcatgatt ttaatagccc ttaacatcaa 29820 ctttctggtg cgatgcgcgc agcaacgcat tctgatttca ctcaaatctt tgcagtaggt 29880 acaacacatt attacaatat tgtttaataa accataatta aaagcgctcc agccaaaact 29940 catatctgat ataatcgccc ctgcatgacc atcataccaa agtttaatat aaattaaatg 30000 acgttccctc aaaaacacac tacccacata catgatctct tttggcatgt gcatattaac 30060 aatctgtctg taccatggac aacgttggtt aatcatgcaa cccaatataa ccttccggaa 30120 ccacactgcc aacaccgctc ccccagccat gcattgaagt gaaccctgct gattacaatg 30180 acaatgaaga acccaattct ctcgaccgtg aatcacttga gaatgaaaaa tatctatagt 30240 ggcacaacat agacataaat gcatgcatct tctcataatt tttaactcct caggatttag 30300 aaacatatcc cagggaatag gaagctcttg cagaacagta aagctggcag aacaaggaag 30360 accacgaaca caacttacac tatgcatagt catagtatca caatctggca acagcgggtg 30420 gtcttcagtc atagaagctc gggtttcatt ttcctcacaa cgtggtaact gggctctggt 30480 gtaagggtga tgtctggcgc atgatgtcga gcgtgcgcgc aaccttgtca taatggagtt 30540 gcttcctgac attctcgtat tttgtatagc aaaacgcggc cctggcagaa cacactcttc 30600 ttcgccttct atcctgccgc ttagcgtgtt ccgtgtgata gttcaagtac aaccacactc 30660 ttaagttggt caaaagaatg ctggcttcag ttgtaatcaa aactccatcg catctaatcg 30720 ttctgaggaa atcatccaag caatgcaact ggattgtgtt tcaagcagga gaggagaggg 30780 aagagacgga agaaccatgt taatttttat tccaaacgat ctcgcagtac ttcaaattgt 30840 agatcgcgca gatggcatct ctcgccccca ctgtgttggt gaaaaagcac agctagatca 30900 aaagaaatgc gattttcaag gtgctcaacg gtggcttcca gcaaagcctc cacgcgcaca 30960 tccaagaaca aaagaatacc aaaagaagga gcattttcta actcctcaat catcatatta 31020 cattcctgca ccattcccag ataattttca gctttccagc cttgaattat tcgtgtcagt 31080 tcttgtggta aatccaatcc acacattaca aacaggtccc ggagggcgcc ctccaccacc 31140 attcttaaac acaccctcat aatgacaaaa tatcttgctc ctgtgtcacc tgtagcgaat 31200 tgagaatggc aacatcaatt gacatgccct tggctctaag ttcttcttta agttctagtt 31260 gtaaaaactc tctcatatta tcaccaaact gcttagccag aagccccccg ggaacaagag 31320 caggggacgc tacagtgcag tacaagcgca gacctcccca attggctcca gcaaaaacaa 31380 gattggaata agcatattgg gaaccgccag taatatcatc gaagttgctg gaaatataat 31440 caggcagagt ttcttgtaaa aattgaataa aagaaaaatt tgccaaaaaa acattcaaaa 31500 cctctgggat gcaaatgcaa taggttaccg cgctgcgctc caacattgtt agttttgaat 31560 tagtctgcaa aaataaaaaa aaaaacaagc gtcatatcat agtagcctga cgaacagatg 31620 gataaatcag tctttccatc acaagacaag ccacagggtc tccagctcga ccctcgtaaa 31680 acctgtcatc atgattaaac aacagcaccg aaagttcctc gcggtgacca gcatgaataa 31740 ttcttgatga agcatacaat ccagacatgt tagcatcagt taacgagaaa aaacagccaa 31800 catagccttt gggtataatt atgcttaatc gtaagtatag caaagccacc cctcgcggat 31860 acaaagtaaa aggcacagga gaataaaaaa tataattatt tctctgctgc tgttcaggca 31920 acgtcgcccc cggtccctct aaatacacat acaaagcctc atcagccatg gcttaccaga 31980 caaagtacag cgggcacaca aagcacaagc tctaaagtga ctctccaacc tctccacaat 32040 atatatatac acaagcccta aactgacgta atgggagtaa agtgtaaaaa atcccgccaa 32100 acccaacaca caccccgaaa ctgcgtcacc agggaaaagt acagtttcac ttccgcaatc 32160 ccaacaggcg taacttcctc tttctcacgg tacgtgatat cccactaact tgcaacgtca 32220 ttttcccacg gtcgcaccgc cccttttagc cgttaacccc acagccaatc accacacgat 32280 ccacactttt taaaatcacc tcatttacat attggcacca ttccatctat aaggtatatt 32340 atatagatag gcgcgccctc tcttaaggta gcatcgggat cgagtccctg agagaacatc 32400 ctcaatcccg atctatcctt agatccgagg aatatcgaaa tcagttacgc tagggataac 32460 agggtaatat agcatcccct cggattgcta tctaccggct cgtcagctat gatctctcga 32520 tttcgatcaa gaaatctcat tggttacctt gggctatcga aaccagtcaa gtcagctact 32580 tggcgagatc gacttgtctg agtttcgact acgctcagaa ttgcgtcagc gcctatcgcc 32640 aggtattact ccaatcccga atatccgagc ctgagagaac atcctcaatc ccgatctatc 32700 cttagatccg aggaatatcg aaatcgttta aatcttttct tgatggtaaa tcattcgaat 32760 ataagaatgg agagacgaat ggggaaacga caaagatgac attctttggt ccttctggtg 32820 aggttctcaa gtttttggtt aatcctgtca acaacttata tcgtatgggg ctgacttcag 32880 gtgctacatt tgaagagata aattgcactg aaatctagta atattttatc tgattaataa 32940 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa 33000 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta 33060 actggcttgg cagagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc 33120 atgacttcaa gactaactct gctaaatcaa ttaccagtgg ctgctgccag tggtgctttt 33180 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac 33240 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt 33300 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa 33360 ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag 33420 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg 33480 gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc 33540 ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga 33600 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc 33660 tgctgacgca ccggtgcggc cttttttctc ctgccacatg aagcacttca ctgacaccct 33720 catcagtgcc aacatagtaa gccagtatac actccgctaa tttaaacgtg gtgtaccgag 33780 aacgatcctc tcagtgcgat ctcgacgatc agtggtattc cgacatatcg ttgcttggca 33840 gtcagccagt cgatcctagc ttgggaccca ggaagtccaa tcgtcagata ttgtactcag 33900 cctggtcacg gcagcgtacc gatctcgtaa ctataacggt cctaaggtag cgaactagat 33960 attgatagtc tgatcggtca acgtataatc gagtcctagc ttttgcaaac atctatcaag 34020 agacaggatc agcaggaggc tttcgcatga ttgaacaaga tggattgcac gcaggttctc 34080 cggcggcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 34140 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgtcc ggttcttttt gtcaagaccg 34200 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcga 34260 cgacgggcgt tccttgcgcg gctgtgctcg acgttgtcac tgaagcggga agggactggc 34320 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 34380 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 34440 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 34500 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 34560 ccaggctcaa ggcgtctatg cccgacggcg aggatctcgt cgtgacccac ggcgatgcct 34620 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccgtc 34680 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 34740 ttggcggcga atgggctgac cgcttccttg tgctttacgg tatcgccgcg cccgattcgc 34800 agcgcatcgc cttctatcgc cttcttgacg agttcttctg accgattcta ggtgcattgg 34860 cgcagaaaaa aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc 34920 agcaacggat acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat 34980 ttatccttaa ggtccgtaac tataacggtc ctaaggtagc gaatcgacct agctctatcg 35040 aatctccctc gtttcgagct tacgcgaact agcctctggc gatagcatcc gaggggcagg 35100 catctatgtc gggtgcggag aaagaggtaa tgtcaagttc gatctgattg cttggcataa 35160 agtccgatgg ttcgagtaga ctcagttcaa cctctctctt aaggtagc 35208 <210> 65 <211> 35231 <212> DNA <213> Artificial Sequence <220> <223> pNG-185 (pColoAd2.6) DNA plasmid, consisting of a bacterial origin of replication (p15A), an antibiotic resistance gene (KanR) and the EnAd genome sequence with inserted unique restriction sites in the BX and BY regions. <400> 65 ggcgcgcctc tatctatata atatacctta tagatggaat ggtgccaata tgtaaatgag 60 gtgattttaa aaagtgtgga tcgtgtggtg attggctgtg gggttaacgg ctaaaagggg 120 cggtgcgacc gtgggaaaat gacgttttgt gggggtggag tttttttgca agttgtcgcg 180 ggaaatgtga cgcataaaaa ggcttttttc tcacggaact acttagtttt cccacggtat 240 ttaacaggaa atgaggtagt tttgaccgga tgcaagtgaa aattgttgat tttcgcgcga 300 aaactgaatg aggaagtgtt tttctgaata atgtggtatt tatggcaggg tggagtattt 360 gttcagggcc aggtagactt tgacccatta cgtggaggtt tcgattaccg tgttttttac 420 ctgaatttcc gcgtaccgtg tcaaagtctt ctgtttttac gtaggtgtca gctgatcgct 480 agggtattta tacctcaggg tttgtgtcaa gaggccactc ttgagtgcca gcgagaagag 540 ttttctcctc tgcgccggca gtttaataat aaaaaaatga gagatttgcg atttctgcct 600 caggaaataa tctctgctga gactggaaat gaaatattgg agcttgtggt gcacgccctg 660 atgggagacg atccggagcc acctgtgcag ctttttgagc ctcctacgct tcaggaactg 720 tatgatttag aggtagaggg atcggaggat tctaatgagg aagctgtaaa tggctttttt 780 accgattcta tgcttttagc tgctaatgaa gggttagaat tagatccgcc tttggacact 840 tttgatactc caggggtaat tgtggaaagc ggtacaggtg taagaaaatt acctgatttg 900 agttccgtgg actgtgattt gcactgctat gaagacgggt ttcctccgag tgatgaggag 960 gaccatgaaa aggagcagtc catgcagact gcagcgggtg agggagtgaa ggctgccaat 1020 gttggttttc agttggattg cccggagctt cctggacatg gctgtaagtc ttgtgaattt 1080 cacaggaaaa atactggagt aaaggaactg ttatgttcgc tttgttatat gagaacgcac 1140 tgccacttta tttacagtaa gtgtgtttaa gttaaaattt aaaggaatat gctgtttttc 1200 acatgtatat tgagtgtgag ttttgtgctt cttattatag gtcctgtgtc tgatgctgat 1260 gaatcaccat ctcctgattc tactacctca cctcctgaga ttcaagcacc tgttcctgtg 1320 gacgtgcgca agcccattcc tgtgaagctt aagcctggga aacgtccagc agtggaaaaa 1380 cttgaggact tgttacaggg tggggacgga cctttggact tgagtacacg gaaacgtcca 1440 agacaataag tgttccatat ccgtgtttac ttaaggtgac gtcaatattt gtgtgacagt 1500 gcaatgtaat aaaaatatgt taactgttca ctggttttta ttgctttttg ggcggggact 1560 caggtatata agtagaagca gacctgtgtg gttagctcat aggagctggc tttcatccat 1620 ggaggtttgg gccattttgg aagaccttag gaagactagg caactgttag agaacgcttc 1680 ggacggagtc tccggttttt ggagattctg gttcgctagt gaattagcta gggtagtttt 1740 taggataaaa caggactata aacaagaatt tgaaaagttg ttggtagatt gcccaggact 1800 ttttgaagct cttaatttgg gccatcaggt tcactttaaa gaaaaagttt tatcagtttt 1860 agacttttca accccaggta gaactgctgc tgctgtggct tttcttactt ttatattaga 1920 taaatggatc ccgcagactc atttcagcag gggatacgtt ttggatttca tagccacagc 1980 attgtggaga acatggaagg ttcgcaagat gaggacaatc ttaggttact ggccagtgca 2040 gcctttgggt gtagcgggaa tcctgaggca tccaccggtc atgccagcgg ttctggagga 2100 ggaacagcaa gaggacaacc cgagagccgg cctggaccct ccagtggagg aggcggagta 2160 gctgacttgt ctcctgaact gcaacgggtg cttactggat ctacgtccac tggacgggat 2220 aggggcgtta agagggagag ggcatctagt ggtactgatg ctagatctga gttggcttta 2280 agtttaatga gtcgcagacg tcctgaaacc atttggtggc atgaggttca gaaagaggga 2340 agggatgaag tttctgtatt gcaggagaaa tattcactgg aacaggtgaa aacatgttgg 2400 ttggagcctg aggatgattg ggaggtggcc attaaaaatt atgccaagat agctttgagg 2460 cctgataaac agtataagat tactagacgg attaatatcc ggaatgcttg ttacatatct 2520 ggaaatgggg ctgaggtggt aatagatact caagacaagg cagttattag atgctgcatg 2580 atggatatgt ggcctggggt agtcggtatg gaagcagtaa cttttgtaaa tgttaagttt 2640 aggggagatg gttataatgg aatagtgttt atggccaata ccaaacttat attgcatggt 2700 tgtagctttt ttggtttcaa caatacctgt gtagatgcct ggggacaggt tagtgtacgg 2760 ggatgtagtt tctatgcgtg ttggattgcc acagctggca gaaccaagag tcaattgtct 2820 ctgaagaaat gcatatttca aagatgtaac ctgggcattc tgaatgaagg cgaagcaagg 2880 gtccgccact gcgcttctac agatactgga tgttttattt tgattaaggg aaatgccagc 2940 gtaaagcata acatgatttg cggtgcttcc gatgagaggc cttatcaaat gctcacttgt 3000 gctggtgggc attgtaatat gctggctact gtgcatattg tttcccatca acgcaaaaaa 3060 tggcctgttt ttgatcacaa tgtgatgacg aagtgtacca tgcatgcagg tgggcgtaga 3120 ggaatgttta tgccttacca gtgtaacatg aatcatgtga aagtgttgtt ggaaccagat 3180 gccttttcca gaatgagcct aacaggaatt tttgacatga acatgcaaat ctggaagatc 3240 ctgaggtatg atgatacgag atcgagggta cgcgcatgcg aatgcggagg caagcatgcc 3300 aggttccagc cggtgtgtgt agatgtgact gaagatctca gaccggatca tttggttatt 3360 gcccgcactg gagcagagtt cggatccagt ggagaagaaa ctgactaagg tgagtattgg 3420 gaaaactttg gggtgggatt ttcagatgga cagattgagt aaaaatttgt tttttctgtc 3480 ttgcagctgt catgagtgga aacgcttctt ttaagggggg agtcttcagc ccttatctga 3540 cagggcgtct cccatcctgg gcaggagttc gtcagaatgt tatgggatct actgtggatg 3600 gaagacccgt ccaacccgcc aattcttcaa cgctgaccta tgctacttta agttcttcac 3660 ctttggacgc agctgcagct gccgccgccg cttctgttgc cgctaacact gtgcttggaa 3720 tgggttacta tggaagcatc atggctaatt ccacttcctc taataaccct tctaccctga 3780 ctcaggacaa gttacttgtc cttttggccc agctggaggc tttgacccaa cgtctgggtg 3840 aactttctca gcaggtggtc gagttgcgag tacaaactga gtctgctgtc ggcacggcaa 3900 agtctaaata aaaaaatccc agaatcaatg aataaataaa caagcttgtt gttgatttaa 3960 aatcaagtgt ttttatttca tttttcgcgc acggtatgcc ctagaccacc gatctctatc 4020 attgagaact cggtggattt tttccaggat cctatagagg tgggattgaa tgtttagata 4080 catgggcatt aggccgtctt tggggtggag atagctccat tgaagggatt catgctccgg 4140 ggtagtgttg taaatcaccc agtcataaca aggtcgcagt gcatggtgtt gcacaatatc 4200 ttttagaagt aggctgattg ccacagataa gcccttggtg taggtgttta caaaccggtt 4260 gagctgggat gggtgcattc ggggtgaaat tatgtgcatt ttggattgga tttttaagtt 4320 ggcaatattg ccgccaagat cccgtcttgg gttcatgtta tgaaggacca ccaagacggt 4380 gtatccggta catttaggaa atttatcgtg cagcttggat ggaaaagcgt ggaaaaattt 4440 ggagacaccc ttgtgtcctc caagattttc catgcactca tccatgataa tagcaatggg 4500 gccgtgggca gcggcgcggg caaacacgtt ccgtgggtct gacacatcat agttatgttc 4560 ctgagttaaa tcatcataag ccattttaat gaatttgggg cggagagtac cagattgggg 4620 tatgaatgtt ccttcgggcc ccggagcata gttcccctca cagatttgca tttcccaagc 4680 tttcagttcc gagggtggaa tcatgtccac ctggggggct atgaaaaaca ccgtttctgg 4740 ggcgggggtg attaattgtg atgatagcaa atttctgagc aattgagatt tgccacatcc 4800 ggtggggcca taaatgattc cgattacggg ttgcaggtgg tagtttaggg aacggcaact 4860 gccgtcttct cgaagcaagg gggccacctc gttcatcatt tcccttacat gcatattttc 4920 ccgcaccaaa tccattagga ggcgctctcc tcctagtgat agaagttctt gtagtgagga 4980 aaagtttttc agcggtttca gaccgtcagc catgggcatt ttggagagag tttgctgcaa 5040 aagttctagt ctgttccaca gttcagtgat gtgttctatg gcatctcgat ccagcagacc 5100 tcctcgtttc gcgggtttgg acggctcctg gaatagggta tgagacgatg ggcgtccagc 5160 gctgccaggg ttcggtcctt ccagggtctc agtgttcgag tcagggttgt ttccgtcaca 5220 gtgaaggggt gtgcgcctgc ttgggcgctt gccagggtgc gcttcagact catcctgctg 5280 gtcgaaaact tctgtcgctt ggcgccctgt atgtcggcca agtagcagtt taccatgagt 5340 tcgtagttga gcgcctcggc tgcgtggcct ttggcgcgga gcttaccttt ggaagttttc 5400 ttgcataccg ggcagtatag gcatttcagc gcatacaact tgggcgcaag gaaaacggat 5460 tctggggagt atgcatctgc gccgcaggag gcgcaaacag tttcacattc caccagccag 5520 gttaaatccg gttcattggg gtcaaaaaca agttttccgc catatttttt gatgcgtttc 5580 ttacctttgg tctccatgag ttcgtgtcct cgttgagtga caaacaggct gtccgtgtcc 5640 ccgtagactg attttacagg cctcttctcc agtggagtgc ctcggtcttc ttcgtacagg 5700 aactctgacc actctgatac aaaggcgcgc gtccaggcca gcacaaagga ggctatgtgg 5760 gaggggtagc gatcgttgtc aaccaggggg tccacctttt ccaaagtatg caaacacatg 5820 tcaccctctt caacatccag gaatgtgatt ggcttgtagg tgtatttcac gtgacctggg 5880 gtccccgctg ggggggtata aaagggggcg gttctttgct cttcctcact gtcttccgga 5940 tcgctgtcca ggaacgtcag ctgttggggt aggtattccc tctcgaaggc gggcatgacc 6000 tctgcactca ggttgtcagt ttctaagaac gaggaggatt tgatattgac agtgccggtt 6060 gagatgcctt tcatgaggtt ttcgtccatc tggtcagaaa acacaatttt tttattgtca 6120 agtttggtgg caaatgatcc atacagggcg ttggataaaa gtttggcaat ggatcgcatg 6180 gtttggttct tttccttgtc cgcgcgctct ttggcggcga tgttgagttg gacatactcg 6240 cgtgccaggc acttccattc ggggaagata gttgttaatt catctggcac gattctcact 6300 tgccaccctc gattatgcaa ggtaattaaa tccacactgg tggccacctc gcctcgaagg 6360 ggttcattgg tccaacagag cctacctcct ttcctagaac agaaaggggg aagtgggtct 6420 agcataagtt catcgggagg gtctgcatcc atggtaaaga ttcccggaag taaatcctta 6480 tcaaaatagc tgatgggagt ggggtcatct aaggccattt gccattctcg agctgccagt 6540 gcgcgctcat atgggttaag gggactgccc catggcatgg gatgggtgag tgcagaggca 6600 tacatgccac agatgtcata gacgtagatg ggatcctcaa agatgcctat gtaggttgga 6660 tagcatcgcc cccctctgat acttgctcgc acatagtcat atagttcatg tgatggcgct 6720 agcagccccg gacccaagtt ggtgcgattg ggtttttctg ttctgtagac gatctggcga 6780 aagatggcgt gagaattgga agagatggtg ggtctttgaa aaatgttgaa atgggcatga 6840 ggtagaccta cagagtctct gacaaagtgg gcataagatt cttgaagctt ggttaccagt 6900 tcggcggtga caagtacgtc tagggcgcag tagtcaagtg tttcttgaat gatgtcataa 6960 cctggttggt ttttcttttc ccacagttcg cggttgagaa ggtattcttc gcgatccttc 7020 cagtactctt ctagcggaaa cccgtctttg tctgcacggt aagatcctag catgtagaac 7080 tgattaactg ccttgtaagg gcagcagccc ttctctacgg gtagagagta tgcttgagca 7140 gcttttcgta gcgaagcgtg agtaagggca aaggtgtctc tgaccatgac tttgaggaat 7200 tggtatttga agtcgatgtc gtcacaggct ccctgttccc agagttggaa gtctacccgt 7260 ttcttgtagg cggggttggg caaagcgaaa gtaacatcat tgaagagaat cttgccggcc 7320 ctgggcatga aattgcgagt gatgcgaaaa ggctgtggta cttccgctcg gttattgata 7380 acctgggcag ctaggacgat ctcgtcgaaa ccgttgatgt tgtgtcctac gatgtataat 7440 tctatgaaac gcggcgtgcc tctgacgtga ggtagcttac tgagctcatc aaaggttagg 7500 tctgtggggt cagataaggc gtagtgttcg agagcccatt cgtgcaggtg aggattcgct 7560 ttaaggaagg aggaccagag gtccactgcc agtgctgttt gtaactggtc ccggtactga 7620 cgaaaatgcc gtccgactgc cattttttct ggggtgacgc aatagaaggt ttgggggtcc 7680 tgccgccagc gatcccactt gagttttatg gcgaggtcat aggcgatgtt gacgagccgc 7740 tggtctccag agagtttcat gaccagcatg aaggggatta gctgcttgcc aaaggacccc 7800 atccaggtgt aggtttccac atcgtaggtg agaaagagcc tttctgtgcg aggatgagag 7860 ccaatcggga agaactggat ctcctgccac cagttggagg aatggctgtt gatgtgatgg 7920 aagtagaact ccctgcgacg cgccgagcat tcatgcttgt gcttgtacag acggccgcag 7980 tagtcgcagc gttgcacggg ttgtatctcg tgaatgagtt gtacctggct tcccttgacg 8040 agaaatttca gtgggaagcc gaggcctggc gattgtatct cgtgctttac tatgttgtct 8100 gcatcggcct gttcatcttc tgtctcgatg gtggtcatgc tgacgagccc tcgcgggagg 8160 caagtccaga cctcggcgcg gcaggggcgg agctcgagga cgagagcgcg caggctggag 8220 ctgtccaggg tcctgagacg ctgcggactc aggttagtag gcagtgtcag gagattaact 8280 tgcatgatct tttggagggc gtgcgggagg ttcagatagt acttgatctc aacgggtccg 8340 ttggtggaga tgtcgatggc ttgcagggtt ccgtgtccct tgggcgctac caccgtgccc 8400 ttgtttttca ttttggacgg cggtggctct gttgcttctt gcatgtttag aagcggtgtc 8460 gagggcgcgc accgggcggc aggggcggct cgggacccgg cggcatggct ggcagtggta 8520 cgtcggcgcc gcgcgcgggt aggttctggt actgcgccct gagaagactc gcatgcgcga 8580 cgacgcggcg gttgacatcc tggatctgac gcctctgggt gaaagctacc ggccccgtga 8640 gcttgaacct gaaagagagt tcaacagaat caatctcggt atcgttgacg gcggcttgcc 8700 taaggatttc ttgcacgtca ccagagttgt cctggtaggc gatctccgcc atgaactgct 8760 cgatctcttc ctcttgaaga tctccgcggc ccgctctctc gacggtggcc gcgaggtcgt 8820 tggagatgcg cccaatgagt tgagagaatg cattcatgcc cgcctcgttc cagacgcggc 8880 tgtagaccac ggcccccacg ggatctctcg cgcgcatgac cacctgggcg aggttgagct 8940 ccacgtggcg ggtgaagacc gcatagttgc ataggcgctg gaaaaggtag ttgagtgtgg 9000 tggcgatgtg ctcggtgacg aagaaataca tgatccatcg tctcagcggc atctcgctga 9060 catcgcccag agcttccaag cgctccatgg cctcgtagaa gtccacggca aaattaaaaa 9120 actgggagtt tcgcgcggac acggtcaact cctcttccag aagacggata agttcggcga 9180 tggtggtgcg cacctcgcgc tcgaaagccc ctgggatttc ttcctcaatc tcttcttctt 9240 ccactaacat ctcttcctct tcaggtgggg ctgcaggagg agggggaacg cggcgacgcc 9300 ggcggcgcac gggcagacgg tcgatgaatc tttcaatgac ctctccgcgg cggcggcgca 9360 tggtttcagt gacggcgcgg ccgttctcgc gcggtcgcag agtaaaaaca ccgccgcgca 9420 tctccttaaa gtggtgactg ggaggttctc cgtttgggag ggagagggcg ctgattatac 9480 attttattaa ttggcccgta gggactgcac gcagagatct gatcgtgtca agatccacgg 9540 gatctgaaaa cctttcgacg aaagcgtcta accagtcaca gtcacaaggt aggctgagta 9600 cggcttcttg tgggcggggg tggttatgtg ttcggtctgg gtcttctgtt tcttcttcat 9660 ctcgggaagg tgagacgatg ctgctggtga tgaaattaaa gtaggcagtt ctaagacggc 9720 ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatacgc aggcgattgg 9780 ccattcccca agcattatcc tgacatctag caagatcttt gtagtagtct tgcatgagcc 9840 gttctacggg cacttcttcc tcacccgttc tgccatgcat acgtgtgagt ccaaatccgc 9900 gcattggttg taccagtgcc aagtcagcta cgactctttc ggcgaggatg gcttgctgta 9960 cttgggtaag ggtggcttga aagtcatcaa aatccacaaa gcggtggtaa gctcctgtat 10020 taatggtgta agcacagttg gccatgactg accagttaac tgtctggtga ccagggcgca 10080 cgagctcggt gtatttaagg cgcgaatagg cgcgggtgtc aaagatgtaa tcgttgcagg 10140 tgcgcaccag atactggtac cctataagaa aatgcggcgg tggttggcgg tagagaggcc 10200 atcgttctgt agctggagcg ccaggggcga ggtcttccaa cataaggcgg tgatagccgt 10260 agatgtacct ggacatccag gtgattcctg cggcggtagt agaagcccga ggaaactcgc 10320 gtacgcggtt ccaaatgttg cgtagcggca tgaagtagtt cattgtaggc acggtttgac 10380 cagtgaggcg cgcgcagtca ttgatgctct atagacacgg agaaaatgaa agcgttcagc 10440 gactcgactc cgtagcctgg aggaacgtga acgggttggg tcgcggtgta ccccggttcg 10500 agacttgtac tcgagccggc cggagccgcg gctaacgtgg tattggcact cccgtctcga 10560 cccagcctac aaaaatccag gatacggaat cgagtcgttt tgctggtttc cgaatggcag 10620 ggaagtgagt cctatttttt ttttttgccg ctcagatgca tcccgtgctg cgacagatgc 10680 gcccccaaca acagcccccc tcgcagcagc agcagcagca atcacaaaag gctgtccctg 10740 caactactgc aactgccgcc gtgagcggtg cgggacagcc cgcctatgat ctggacttgg 10800 aagagggcga aggactggca cgtctaggtg cgccttcacc cgagcggcat ccgcgagttc 10860 aactgaaaaa agattctcgc gaggcgtatg tgccccaaca gaacctattt agagacagaa 10920 gcggcgagga gccggaggag atgcgagctt cccgctttaa cgcgggtcgt gagctgcgtc 10980 acggtttgga ccgaagacga gtgttgcggg acgaggattt cgaagttgat gaaatgacag 11040 ggatcagtcc tgccagggca cacgtggctg cagccaacct tgtatcggct tacgagcaga 11100 cagtaaagga agagcgtaac ttccaaaagt cttttaataa tcatgtgcga accctgattg 11160 cccgcgaaga agttaccctt ggtttgatgc atttgtggga tttgatggaa gctatcattc 11220 agaaccctac tagcaaacct ctgaccgccc agctgtttct ggtggtgcaa cacagcagag 11280 acaatgaggc tttcagagag gcgctgctga acatcaccga acccgagggg agatggttgt 11340 atgatcttat caacattcta cagagtatca tagtgcagga gcggagcctg ggcctggccg 11400 agaaggtggc tgccatcaat tactcggttt tgagcttggg aaaatattac gctcgcaaaa 11460 tctacaagac tccatacgtt cccatagaca aggaggtgaa gatagatggg ttctacatgc 11520 gcatgacgct caaggtcttg accctgagcg atgatcttgg ggtgtatcgc aatgacagaa 11580 tgcatcgcgc ggttagcgcc agcaggaggc gcgagttaag cgacagggaa ctgatgcaca 11640 gtttgcaaag agctctgact ggagctggaa ccgagggtga gaattacttc gacatgggag 11700 ctgacttgca gtggcagcct agtcgcaggg ctctgagcgc cgcgacggca ggatgtgagc 11760 ttccttacat agaagaggcg gatgaaggcg aggaggaaga gggcgagtac ttggaagact 11820 gatggcacaa cccgtgtttt ttgctagatg gaacagcaag caccggatcc cgcaatgcgg 11880 gcggcgctgc agagccagcc gtccggcatt aactcctcgg acgattggac ccaggccatg 11940 caacgtatca tggcgttgac gactcgcaac cccgaagcct ttagacagca accccaggcc 12000 aaccgtctat cggccatcat ggaagctgta gtgccttccc gctctaatcc cactcatgag 12060 aaggtcctgg ccatcgtgaa cgcgttggtg gagaacaaag ctattcgtcc agatgaggcc 12120 ggactggtat acaacgctct cttagaacgc gtggctcgct acaacagtag caatgtgcaa 12180 accaatttgg accgtatgat aacagatgta cgcgaagccg tgtctcagcg cgaaaggttc 12240 cagcgtgatg ccaacctggg ttcgctggtg gcgttaaatg ctttcttgag tactcagcct 12300 gctaatgtgc cgcgtggtca acaggattat actaactttt taagtgcttt gagactgatg 12360 gtatcagaag tacctcagag cgaagtgtat cagtccggtc ctgattactt ctttcagact 12420 agcagacagg gcttgcagac ggtaaatctg agccaagctt ttaaaaacct taaaggtttg 12480 tggggagtgc atgccccggt aggagaaaga gcaaccgtgt ctagcttgtt aactccgaac 12540 tcccgcctat tattactgtt ggtagctcct ttcaccgaca gcggtagcat cgaccgtaat 12600 tcctatttgg gttacctact aaacctgtat cgcgaagcca tagggcaaag tcaggtggac 12660 gagcagacct atcaagaaat tacccaagtc agtcgcgctt tgggacagga agacactggc 12720 agtttggaag ccactctgaa cttcttgctt accaatcggt ctcaaaagat ccctcctcaa 12780 tatgctctta ctgcggagga ggagaggatc cttagatatg tgcagcagag cgtgggattg 12840 tttctgatgc aagagggggc aactccgact gcagcactgg acatgacagc gcgaaatatg 12900 gagcccagca tgtatgccag taaccgacct ttcattaaca aactgctgga ctacttgcac 12960 agagctgccg ctatgaactc tgattatttc accaatgcca tcttaaaccc gcactggctg 13020 cccccacctg gtttctacac gggcgaatat gacatgcccg accctaatga cggatttctg 13080 tgggacgacg tggacagcga tgttttttca cctctttctg atcatcgcac gtggaaaaag 13140 gaaggcggcg atagaatgca ttcttctgca tcgctgtccg gggtcatggg tgctaccgcg 13200 gctgagcccg agtctgcaag tccttttcct agtctaccct tttctctaca cagtgtacgt 13260 agcagcgaag tgggtagaat aagtcgcccg agtttaatgg gcgaagagga gtatctaaac 13320 gattccttgc tcagaccggc aagagaaaaa aatttcccaa acaatggaat agaaagtttg 13380 gtggataaaa tgagtagatg gaagacttat gctcaggatc acagagacga gcctgggatc 13440 atggggatta caagtagagc gagccgtaga cgccagcgcc atgacagaca gaggggtctt 13500 gtgtgggacg atgaggattc ggccgatgat agcagcgtgc tggacttggg tgggagagga 13560 aggggcaacc cgtttgctca tttgcgccct cgcttgggtg gtatgttgta aaaaaaaata 13620 aaaaaaaaac tcaccaaggc catggcgacg agcgtacgtt cgttcttctt tattatctgt 13680 gtctagtata atgaggcgag tcgtgctagg cggagcggtg gtgtatccgg agggtcctcc 13740 tccttcgtac gagagcgtga tgcagcagca gcaggcgacg gcggtgatgc aatccccact 13800 ggaggctccc tttgtgcctc cgcgatacct ggcacctacg gagggcagaa acagcattcg 13860 ttattcggaa ctggcacctc agtacgatac caccaggttg tatctggtgg acaacaagtc 13920 ggcggacatt gcttctctga actatcagaa tgaccacagc aacttcttga ccacggtggt 13980 gcaaaacaat gactttaccc ctacggaagc cagcacccag accattaact ttgatgaacg 14040 atcgcggtgg ggcggtcagc taaagaccat catgcatact aacatgccaa acgtgaacga 14100 gtatatgttt agtaacaagt tcaaagcgcg tgtgatggtg tccagaaaac ctcccgacgg 14160 tgctgcagtt ggggatactt atgatcacaa gcaggatatt ttgaaatatg agtggttcga 14220 gtttactttg ccagaaggca acttttcagt tactatgact attgatttga tgaacaatgc 14280 catcatagat aattacttga aagtgggtag acagaatgga gtgcttgaaa gtgacattgg 14340 tgttaagttc gacaccagga acttcaagct gggatgggat cccgaaacca agttgatcat 14400 gcctggagtg tatacgtatg aagccttcca tcctgacatt gtcttactgc ctggctgcgg 14460 agtggatttt accgagagtc gtttgagcaa ccttcttggt atcagaaaaa aacagccatt 14520 tcaagagggt tttaagattt tgtatgaaga tttagaaggt ggtaatattc cggccctctt 14580 ggatgtagat gcctatgaga acagtaagaa agaacaaaaa gccaaaatag aagctgctac 14640 agctgctgca gaagctaagg caaacatagt tgccagcgac tctacaaggg ttgctaacgc 14700 tggagaggtc agaggagaca attttgcgcc aacacctgtt ccgactgcag aatcattatt 14760 ggccgatgtg tctgaaggaa cggacgtgaa actcactatt caacctgtag aaaaagatag 14820 taagaataga agctataatg tgttggaaga caaaatcaac acagcctatc gcagttggta 14880 tctttcgtac aattatggcg atcccgaaaa aggagtgcgt tcctggacat tgctcaccac 14940 ctcagatgtc acctgcggag cagagcaggt ctactggtcg cttccagaca tgatgaagga 15000 tcctgtcact ttccgctcca ctagacaagt cagtaactac cctgtggtgg gtgcagagct 15060 tatgcccgtc ttctcaaaga gcttctacaa cgaacaagct gtgtactccc agcagctccg 15120 ccagtccacc tcgcttacgc acgtcttcaa ccgctttcct gagaaccaga ttttaatccg 15180 tccgccggcg cccaccatta ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg 15240 gaccctgccg ttgcgcagca gtatccgggg agtccaacgt gtgaccgtta ctgacgccag 15300 acgccgcacc tgtccctacg tgtacaaggc actgggcata gtcgcaccgc gcgtcctttc 15360 aagccgcact ttctaaaaaa aaaaaaaatg tccattctta tctcgcccag taataacacc 15420 ggttggggtc tgcgcgctcc aagcaagatg tacggaggcg cacgcaaacg ttctacccaa 15480 catcctgtcc gtgttcgcgg acattttcgc gctccatggg gcgccctcaa gggccgcact 15540 cgcgttcgaa ccaccgtcga tgatgtaatc gatcaggtgg ttgccgacgc ccgtaattat 15600 actcctactg cgcctacatc tactgtggat gcagttattg acagtgtagt ggctgacgct 15660 cgcaactatg ctcgacgtaa gagccggcga aggcgcattg ccagacgcca ccgagctacc 15720 actgccatgc gagccgcaag agctctgcta cgaagagcta gacgcgtggg gcgaagagcc 15780 atgcttaggg cggccagacg tgcagcttcg ggcgccagcg ccggcaggtc ccgcaggcaa 15840 gcagccgctg tcgcagcggc gactattgcc gacatggccc aatcgcgaag aggcaatgta 15900 tactgggtgc gtgacgctgc caccggtcaa cgtgtacccg tgcgcacccg tccccctcgc 15960 acttagaaga tactgagcag tctccgatgt tgtgtcccag cggcgaggat gtccaagcgc 16020 aaatacaagg aagaaatgct gcaggttatc gcacctgaag tctacggcca accgttgaag 16080 gatgaaaaaa aaccccgcaa aatcaagcgg gttaaaaagg acaaaaaaga agaggaagat 16140 ggcgatgatg ggctggcgga gtttgtgcgc gagtttgccc cacggcgacg cgtgcaatgg 16200 cgtgggcgca aagttcgaca tgtgttgaga cctggaactt cggtggtctt tacacccggc 16260 gagcgttcaa gcgctacttt taagcgttcc tatgatgagg tgtacgggga tgatgatatt 16320 cttgagcagg cggctgaccg attaggcgag tttgcttatg gcaagcgtag tagaataact 16380 tccaaggatg agacagtgtc gatacccttg gatcatggaa atcccacccc tagtcttaaa 16440 ccggtcactt tgcagcaagt gttacccgta actccgcgaa caggtgttaa acgcgaaggt 16500 gaagatttgt atcccactat gcaactgatg gtacccaaac gccagaagtt ggaggacgtt 16560 ttggagaaag taaaagtgga tccagatatt caacctgagg ttaaagtgag acccattaag 16620 caggtagcgc ctggtctggg ggtacaaact gtagacatta agattcccac tgaaagtatg 16680 gaagtgcaaa ctgaacccgc aaagcctact gccacctcca ctgaagtgca aacggatcca 16740 tggatgccca tgcctattac aactgacgcc gccggtccca ctcgaagatc ccgacgaaag 16800 tacggtccag caagtctgtt gatgcccaat tatgttgtac acccatctat tattcctact 16860 cctggttacc gaggcactcg ctactatcgc agccgaaaca gtacctcccg ccgtcgccgc 16920 aagacacctg caaatcgcag tcgtcgccgt agacgcacaa gcaaaccgac tcccggcgcc 16980 ctggtgcggc aagtgtaccg caatggtagt gcggaacctt tgacactgcc gcgtgcgcgt 17040 taccatccga gtatcatcac ttaatcaatg ttgccgctgc ctccttgcag atatggccct 17100 cacttgtcgc cttcgcgttc ccatcactgg ttaccgagga agaaactcgc gccgtagaag 17160 agggatgttg ggacgcggaa tgcgacgcta caggcgacgg cgtgctatcc gcaagcaatt 17220 gcggggtggt tttttaccag ccttaattcc aattatcgct gctgcaattg gcgcgatacc 17280 aggcatagct tccgtggcgg ttcaggcctc gcaacgacat tgacattgga aaaaaacgta 17340 taaataaaaa aaaaaaaata caatggactc tgacactcct ggtcctgtga ctatgttttc 17400 ttagagatgg aagacatcaa tttttcatcc ttggctccgc gacacggcac gaagccgtac 17460 atgggcacct ggagcgacat cggcacgagc caactgaacg ggggcgcctt caattggagc 17520 agtatctgga gcgggcttaa aaattttggc tcaaccataa aaacatacgg gaacaaagct 17580 tggaacagca gtacaggaca ggcgcttaga aataaactta aagaccagaa cttccaacaa 17640 aaagtagtcg atgggatagc ttccggcatc aatggagtgg tagatttggc taaccaggct 17700 gtgcagaaaa agataaacag tcgtttggac ccgccgccag caaccccagg tgaaatgcaa 17760 gtggaggaag aaattcctcc gccagaaaaa cgaggcgaca agcgtccgcg tcccgatttg 17820 gaagagacgc tggtgacgcg cgtagatgaa ccgccttctt atgaggaagc aacgaagctt 17880 ggaatgccca ccactagacc gatagcccca atggccaccg gggtgatgaa accttctcag 17940 ttgcatcgac ccgtcacctt ggatttgccc cctccccctg ctgctactgc tgtacccgct 18000 tctaagcctg tcgctgcccc gaaaccagtc gccgtagcca ggtcacgtcc cgggggcgct 18060 cctcgtccaa atgcgcactg gcaaaatact ctgaacagca tcgtgggtct aggcgtgcaa 18120 agtgtaaaac gccgtcgctg cttttaatta aatatggagt agcgcttaac ttgcctatct 18180 gtgtatatgt gtcattacac gccgtcacag cagcagagga aaaaaggaag aggtcgtgcg 18240 tcgacgctga gttactttca agatggccac cccatcgatg ctgccccaat gggcatacat 18300 gcacatcgcc ggacaggatg cttcggagta cctgagtccg ggtctggtgc agttcgcccg 18360 cgccacagac acctacttca atctgggaaa taagtttaga aatcccaccg tagcgccgac 18420 ccacgatgtg accaccgacc gtagccagcg gctcatgttg cgcttcgtgc ccgttgaccg 18480 ggaggacaat acatactctt acaaagtgcg gtacaccctg gccgtgggcg acaacagagt 18540 gctggatatg gccagcacgt tctttgacat taggggtgtg ttggacagag gtcccagttt 18600 caaaccctat tctggtacgg cttacaactc cctggctcct aaaggcgctc caaatacatc 18660 tcagtggatt gcagaaggtg taaaaaatac aactggtgag gaacacgtaa cagaagagga 18720 aaccaatact actacttaca cttttggcaa tgctcctgta aaagctgaag ctgaaattac 18780 aaaagaagga ctcccagtag gtttggaagt ttcagatgaa gaaagtaaac cgatttatgc 18840 tgataaaaca tatcagccag aacctcagct gggagatgaa acttggactg accttgatgg 18900 aaaaaccgaa aagtatggag gcagggctct caaacccgat actaagatga aaccatgcta 18960 cgggtccttt gccaaaccta ctaatgtgaa aggcggtcag gcaaaacaaa aaacaacgga 19020 gcagccaaat cagaaagtcg aatatgatat cgacatggag ttttttgatg cggcatcgca 19080 gaaaacaaac ttaagtccta aaattgtcat gtatgcagaa aatgtaaatt tggaaactcc 19140 agacactcat gtagtgtaca aacctggaac agaagacaca agttccgaag ctaatttggg 19200 acaacaatct atgcccaaca gacccaacta cattggcttc agagataact ttattggact 19260 tatgtactat aacagtactg gtaacatggg ggtgctggct ggtcaagcgt ctcagttaaa 19320 tgcagtggtt gacttgcagg acagaaacac agaactttct taccaactct tgcttgactc 19380 tctgggcgac agaaccagat actttagcat gtggaatcag gctgtggaca gttatgatcc 19440 tgatgtacgt gttattgaaa atcatggtgt ggaagatgaa cttcccaact actgttttcc 19500 actggacggc ataggtgttc caacaaccag ttacaaatca atagttccaa atggagacaa 19560 tgcgcctaat tggaaggaac ctgaagtaaa tggaacaagt gagatcggac agggtaattt 19620 gtttgccatg gaaattaacc ttcaagccaa tctatggcga agtttccttt attccaatgt 19680 ggctctatat ctcccagact cgtacaaata caccccgtcc aatgtcactc ttccagaaaa 19740 caaaaacacc tacgactaca tgaacgggcg ggtggtgccg ccatctctag tagacaccta 19800 tgtgaacatt ggtgccaggt ggtctctgga tgccatggac aatgtcaacc cattcaacca 19860 ccaccgtaac gctggcttgc gttaccgatc catgcttctg ggtaacggac gttatgtgcc 19920 tttccacata caagtgcctc aaaaattctt cgctgttaaa aacctgctgc ttctcccagg 19980 ctcctacact tatgagtgga actttaggaa ggatgtgaac atggttctac agagttccct 20040 cggtaacgac ctgcgggtag atggcgccag catcagtttc acgagcatca acctctatgc 20100 tacttttttc cccatggctc acaacaccgc ttccaccctt gaagccatgc tgcggaatga 20160 caccaatgat cagtcattca acgactacct atctgcagct aacatgctct accccattcc 20220 tgccaatgca accaatattc ccatttccat tccttctcgc aactgggcgg ctttcagagg 20280 ctggtcattt accagactga aaaccaaaga aactccctct ttggggtctg gatttgaccc 20340 ctactttgtc tattctggtt ctattcccta cctggatggt accttctacc tgaaccacac 20400 ttttaagaag gtttccatca tgtttgactc ttcagtgagc tggcctggaa atgacaggtt 20460 actatctcct aacgaatttg aaataaagcg cactgtggat ggcgaaggct acaacgtagc 20520 ccaatgcaac atgaccaaag actggttctt ggtacagatg ctcgccaact acaacatcgg 20580 ctatcagggc ttctacattc cagaaggata caaagatcgc atgtattcat ttttcagaaa 20640 cttccagccc atgagcaggc aggtggttga tgaggtcaat tacaaagact tcaaggccgt 20700 cgccataccc taccaacaca acaactctgg ctttgtgggt tacatggctc cgaccatgcg 20760 ccaaggtcaa ccctatcccg ctaactatcc ctatccactc attggaacaa ctgccgtaaa 20820 tagtgttacg cagaaaaagt tcttgtgtga cagaaccatg tggcgcatac cgttctcgag 20880 caacttcatg tctatggggg cccttacaga cttgggacag aatatgctct atgccaactc 20940 agctcatgct ctggacatga cctttgaggt ggatcccatg gatgagccca ccctgcttta 21000 tcttctcttc gaagttttcg acgtggtcag agtgcatcag ccacaccgcg gcatcatcga 21060 ggcagtctac ctgcgtacac cgttctcggc cggtaacgct accacgtaag aagcttcttg 21120 cttcttgcaa atagcagctg caaccatggc ctgcggatcc caaaacggct ccagcgagca 21180 agagctcaga gccattgtcc aagacctggg ttgcggaccc tattttttgg gaacctacga 21240 taagcgcttc ccggggttca tggcccccga taagctcgcc tgtgccattg taaatacggc 21300 cggacgtgag acggggggag agcactggtt ggctttcggt tggaacccac gttctaacac 21360 ctgctacctt tttgatcctt ttggattctc ggatgatcgt ctcaaacaga tttaccagtt 21420 tgaatatgag ggtctcctgc gccgcagcgc tcttgctacc aaggaccgct gtattacgct 21480 ggaaaaatct acccagaccg tgcagggtcc ccgttctgcc gcctgcggac ttttctgctg 21540 catgttcctt cacgcctttg tgcactggcc tgaccgtccc atggacggaa accccaccat 21600 gaaattgcta actggagtgc caaacaacat gcttcattct cctaaagtcc agcccaccct 21660 gtgtgacaat caaaaagcac tctaccattt tcttaatacc cattcgcctt attttcgctc 21720 ccatcgtaca cacatcgaaa gggccactgc gttcgaccgt atggatgttc aataatgact 21780 catgtaaaca acgtgttcaa taaacatcac tttatttttt tacatgtatc aaggctctgc 21840 attacttatt tatttacaag tcgaatgggt tctgacgaga atcagaatga cccgcaggca 21900 gtgatacgtt gcggaactga tacttgggtt gccacttgaa ttcgggaatc accaacttgg 21960 gaaccggtat atcgggcagg atgtcactcc acagctttct ggtcagctgc aaagctccaa 22020 gcaggtcagg agccgaaatc ttgaaatcac aattaggacc agtgctttga gcgcgagagt 22080 tgcggtacac cggattgcag cactgaaaca ccatcagcga cggatgtctc acgcttgcca 22140 gcacggtggg atctgcaatc atgcccacat ccagatcttc agcattggca atgctgaacg 22200 gggtcatctt gcaggtctgc ctacccatgg cgggcaccca attaggcttg tggttgcaat 22260 cgcagtgcag ggggatcagt atcatcttgg cctgatcctg tctgattcct ggatacacgg 22320 ctctcatgaa agcatcatat tgcttgaaag cctgctgggc tttactaccc tcggtataaa 22380 acatcccgca ggacctgctc gaaaactggt tagctgcaca gccggcatca ttcacacagc 22440 agcgggcgtc attgttagct atttgcacca cacttctgcc ccagcggttt tgggtgattt 22500 tggttcgctc gggattctcc tttaaggctc gttgtccgtt ctcgctggcc acatccatct 22560 cgataatctg ctccttctga atcataatat tgccatgcag gcacttcagc ttgccctcat 22620 aatcattgca gccatgaggc cacaacgcac agcctgtaca ttcccaatta tggtgggcga 22680 tctgagaaaa agaatgtatc attccctgca gaaatcttcc catcatcgtg ctcagtgtct 22740 tgtgactagt gaaagttaac tggatgcctc ggtgctcctc gtttacgtac tggtgacaga 22800 tgcgcttgta ttgttcgtgt tgctcaggca ttagtttaaa agaggttcta agttcgttat 22860 ccagcctgta cttctccatc agcagacaca tcacttccat gcctttctcc caagcagaca 22920 ccaggggcaa gctaatcgga ttcttaacag tgcaggcagc agctccttta gccagagggt 22980 catctttagc gatcttctca atgcttcttt tgccatcctt ctcaacgatg cgcacgggcg 23040 ggtagctgaa acccactgct acaagttgcg cctcttctct ttcttcttcg ctgtcttgac 23100 tgatgtcttg catggggata tgtttggtct tccttggctt ctttttgggg ggtatcggag 23160 gaggaggact gtcgctccgt tccggagaca gggaggattg tgacgtttcg ctcaccatta 23220 ccaactgact gtcggtagaa gaacctgacc ccacacggcg acaggtgttt ctcttcgggg 23280 gcagaggtgg aggcgattgc gaagggctgc ggtccgacct ggaaggcgga tgactggcag 23340 aaccccttcc gcgttcgggg gtgtgctccc tgtggcggtc gcttaactga tttccttcgc 23400 ggctggccat tgtgttctcc taggcagaga aacaacagac atggaaactc agccattgct 23460 gtcaacatcg ccacgagtgc catcacatct cgtcctcagc gacgaggaaa aggagcagag 23520 cttaagcatt ccaccgccca gtcctgccac cacctctacc ctagaagata aggaggtcga 23580 cgcatctcat gacatgcaga ataaaaaagc gaaagagtct gagacagaca tcgagcaaga 23640 cccgggctat gtgacaccgg tggaacacga ggaagagttg aaacgctttc tagagagaga 23700 ggatgaaaac tgcccaaaac aacgagcaga taactatcac caagatgctg gaaataggga 23760 tcagaacacc gactacctca tagggcttga cggggaagac gcgctcctta aacatctagc 23820 aagacagtcg ctcatagtca aggatgcatt attggacaga actgaagtgc ccatcagtgt 23880 ggaagagctc agccgcgcct acgagcttaa cctcttttca cctcgtactc cccccaaacg 23940 tcagccaaac ggcacctgcg agccaaatcc tcgcttaaac ttttatccag cttttgctgt 24000 gccagaagta ctggctacct atcacatctt ttttaaaaat caaaaaattc cagtctcctg 24060 ccgcgctaat cgcacccgcg ccgatgccct actcaatctg ggacctggtt cacgcttacc 24120 tgatatagct tccttggaag aggttccaaa gatcttcgag ggtctgggca ataatgagac 24180 tcgggccgca aatgctctgc aaaagggaga aaatggcatg gatgagcatc acagcgttct 24240 ggtggaattg gaaggcgata atgccagact cgcagtactc aagcgaagca tcgaggtcac 24300 acacttcgca tatcccgctg tcaacctgcc ccctaaagtc atgacggcgg tcatggacca 24360 gttactcatt aagcgcgcaa gtcccctttc agaagacatg catgacccag atgcctgtga 24420 tgagggtaaa ccagtggtca gtgatgagca gctaacccga tggctgggca ccgactctcc 24480 cagggatttg gaagagcgtc gcaagcttat gatggccgtg gtgctggtta ccgtagaact 24540 agagtgtctc cgacgtttct ttaccgattc agaaaccttg cgcaaactcg aagagaatct 24600 gcactacact tttagacacg gctttgtgcg gcaggcatgc aagatatcta acgtggaact 24660 caccaacctg gtttcctaca tgggtattct gcatgagaat cgcctaggac aaagcgtgct 24720 gcacagcacc ctgaaggggg aagcccgccg tgattacatc cgcgattgtg tctatctgta 24780 cctgtgccac acgtggcaaa ccggcatggg tgtatggcag caatgtttag aagaacagaa 24840 cttgaaagag cttgacaagc tcttacagaa atctcttaag gttctgtgga cagggttcga 24900 cgagcgcacc gtcgcttccg acctggcaga cctcatcttc ccagagcgtc tcagggttac 24960 tttgcgaaac ggattgcctg actttatgag ccagagcatg cttaacaatt ttcgctcttt 25020 catcctggaa cgctccggta tcctgcccgc cacctgctgc gcactgccct ccgactttgt 25080 gcctctcacc taccgcgagt gccccccgcc gctatggagt cactgctacc tgttccgtct 25140 ggccaactat ctctcctacc actcggatgt gatcgaggat gtgagcggag acggcttgct 25200 ggagtgtcac tgccgctgca atctgtgcac gccccaccgg tccctagctt gcaaccccca 25260 gttgatgagc gaaacccaga taataggcac ctttgaattg caaggcccca gcagccaagg 25320 cgatgggtct tctcctgggc aaagtttaaa actgaccccg ggactgtgga cctccgccta 25380 cttgcgcaag tttgctccgg aagattacca cccctatgaa atcaagttct atgaggacca 25440 atcacagcct ccaaaggccg aactttcggc ctgcgtcatc acccaggggg caattctggc 25500 ccaattgcaa gccatccaaa aatcccgcca agaatttcta ctgaaaaagg gtaagggggt 25560 ctaccttgac ccccagaccg gcgaggaact caacacaagg ttccctcagg atgtcccaac 25620 gacgagaaaa caagaagttg aaggtgcagc cgccgccccc agaagatatg gaggaagatt 25680 gggacagtca ggcagaggag gcggaggagg acagtctgga ggacagtctg gaggaagaca 25740 gtttggagga ggaaaacgag gaggcagagg aggtggaaga agtaaccgcc gacaaacagt 25800 tatcctcggc tgcggagaca agcaacagcg ctaccatctc cgctccgagt cgaggaaccc 25860 ggcggcgtcc cagcagtaga tgggacgaga ccggacgctt cccgaaccca accagcgctt 25920 ccaagaccgg taagaaggat cggcagggat acaagtcctg gcgggggcat aagaatgcca 25980 tcatctcctg cttgcatgag tgcgggggca acatatcctt cacgcggcgc tacttgctat 26040 tccaccatgg ggtgaacttt ccgcgcaatg ttttgcatta ctaccgtcac ctccacagcc 26100 cctactatag ccagcaaatc ccggcagtct cgacagataa agacagcggc ggcgacctcc 26160 aacagaaaac cagcagcggc agttagaaaa tacacaacaa gtgcagcaac aggaggatta 26220 aagattacag ccaacgagcc agcgcaaacc cgagagttaa gaaatcggat ctttccaacc 26280 ctgtatgcca tcttccagca gagtcggggt caagagcagg aactgaaaat aaaaaaccga 26340 tctctgcgtt cgctcaccag aagttgtttg tatcacaaga gcgaagatca acttcagcgc 26400 actctcgagg acgccgaggc tctcttcaac aagtactgcg cgctgactct taaagagtag 26460 gcagcgaccg cgcttattca aaaaaggcgg gaattacatc atcctcgaca tgagtaaaga 26520 aattcccacg ccttacatgt ggagttatca accccaaatg ggattggcgg caggcgcctc 26580 ccaggactac tccacccgca tgaattggct cagcgccggg ccttctatga tttctcgagt 26640 taatgatata cgcgcctacc gaaaccaaat acttttggaa cagtcagctc ttaccaccac 26700 gccccgccaa caccttaatc ccagaaattg gcccgccgcc ctagtgtacc aggaaagtcc 26760 cgctcccacc actgtattac ttcctcgaga cgcccaggcc gaagtccaaa tgactaatgc 26820 aggtgcgcag ttagctggcg gctccaccct atgtcgtcac aggcctcggc ataatataaa 26880 acgcctgatg atcagaggcc gaggtatcca gctcaacgac gagtcggtga gctctccgct 26940 tggtctacga ccagacggaa tctttcagat tgccggctgc gggagatctt ccttcacccc 27000 tcgtcaggct gttctgactt tggaaagttc gtcttcgcaa ccccgctcgg gcggaatcgg 27060 gaccgttcaa tttgtggagg agtttactcc ctctgtctac ttcaacccct tctccggatc 27120 tcctgggcat tacccggacg agttcatacc gaacttcgac gcgattagcg agtcagtgga 27180 cggctacgat tgatgtctgg tgacgcggct gagctatctc ggctgcgaca tctagaccac 27240 tgccgccgct ttcgctgctt tgcccgggaa ctcattgagt tcatctactt cgaactcccc 27300 aaggatcacc ctcaaggtcc ggcccacgga gtgcggattt ctatcgaagg caaaatagac 27360 tctcgcctgc aacgaatttt ctcccagcgg cccgtgctga tcgagcgaga ccagggaaac 27420 accacggttt ccatctactg catttgtaat caccccggat tgcatgaaag cctttgctgt 27480 cttatgtgta ctgagtttaa taaaaactga attaagactc tcctacggac tgccgcttct 27540 tcaacccgga ttttacaacc agaagaacga aacttttcct gtcgtccagg actctgttaa 27600 cttcaccttt cctactcaca aactagaagc tcaacgacta caccgctttt ccagaagcat 27660 tttccctact aatactactt tcaaaaccgg aggtgagctc caaggtcttc ctacagaaaa 27720 cccttgggtg gaagcgggcc ttgtagtgct aggaattctt gcgggtgggc ttgtgattat 27780 tctttgctac ctatacacac cttgcttcac tttcttagtg gtgttgtggt attggtttaa 27840 aaaatggggc ccatactagt cttgcttgtt ttactttcgc ttttggaacc gggttctgcc 27900 aattacgatc catgtctaga cttcgaccca gaaaactgca cacttacttt tgcacccgac 27960 acaagccgca tctgtggagt tcatcgcctc tcttacgaac ttggccccca acgacaaaaa 28020 tttacctgca tggtgggaat caaccccata gttatcaccc agcaaagtgg agatactaag 28080 ggttgcattc actgctcctg cgattccatc gagtgcacct acaccctgct gaagacccta 28140 tgcggcctaa gagacctgct accaatgaat taaaaaatga ttaataaaaa atcacttact 28200 gcggccgcta tggccggcct gaaatcagca ataaggtctc tgttgaaatt ttctcccagc 28260 agcacctcac ttccctcttc ccaactctgg tattctaaac cccgttcagc ggcatacttt 28320 ctccatactt taaaggggat gtcaaatttt agctcctctc ctgtacccac aatcttcatg 28380 tctttcttcc cagatgacca agagagtccg gctcagtgac tccttcaacc ctgtctaccc 28440 ctatgaagat gaaagcacct cccaacaccc ctttataaac ccagggttta tttccccaaa 28500 tggcttcaca caaagcccaa acggagttct tactttaaaa tgtttaaccc cactaacaac 28560 cacaggcgga tctctacagc taaaagtggg agggggactt acagtggatg acaccaacgg 28620 ttttttgaaa gaaaacataa gtgccaccac accactcgtt aagactggtc actctatagg 28680 tttaccacta ggagccggat tgggaacgaa tgaaaataaa ctttgtatca aattaggaca 28740 aggacttaca ttcaattcaa acaacatttg cattgatgac aatattaaca ccttatggac 28800 aggagtcaac cccaccgaag ccaactgtca aatcatgaac tccagtgaat ctaatgattg 28860 caaattaatt ctaacactag ttaaaactgg agcactagtc actgcatttg tttatgttat 28920 aggagtatct aacaatttta atatgctaac tacacacaga aatataaatt ttactgcaga 28980 gctgtttttc gattctactg gtaatttact aactagactc tcatccctca aaactccact 29040 taatcataaa tcaggacaaa acatggctac tggtgccatt actaatgcta aaggtttcat 29100 gcccagcacg actgcctatc ctttcaatga taattctaga gaaaaagaaa actacattta 29160 cggaacttgt tactacacag ctagtgatcg cactgctttt cccattgaca tatctgtcat 29220 gcttaaccga agagcaataa atgacgagac atcatattgt attcgtataa cttggtcctg 29280 gaacacagga gatgccccag aggtgcaaac ctctgctaca accctagtca cctccccatt 29340 taccttttac tacatcagag aagacgactg acaaataaag tttgcgatcg ctaccctgca 29400 ggaacttgtt tatttgaaaa tcaattcaca aaatccgagt agttattttg cctccccctt 29460 cccatttaac agaatacacc aatctctccc cacgcacagc tttaaacatt tggataccat 29520 tagatataga catggtttta gattccacat tccaaacagt ttcagagcga gccaatctgg 29580 ggtcagtgat agataaaaat ccatcgggat agtcttttaa agcgctttca cagtccaact 29640 gctgcggatg cgactccgga gtctggatca cggtcatctg gaagaagaac gatgggaatc 29700 ataatccgaa aacggtatcg gacgattgtg tctcatcaaa cccacaagca gccgctgtct 29760 gcgtcgctcc gtgcgactgc tgtttatggg atcagggtcc acagtgtcct gaagcatgat 29820 tttaatagcc cttaacatca actttctggt gcgatgcgcg cagcaacgca ttctgatttc 29880 actcaaatct ttgcagtagg tacaacacat tattacaata ttgtttaata aaccataatt 29940 aaaagcgctc cagccaaaac tcatatctga tataatcgcc cctgcatgac catcatacca 30000 aagtttaata taaattaaat gacgttccct caaaaacaca ctacccacat acatgatctc 30060 ttttggcatg tgcatattaa caatctgtct gtaccatgga caacgttggt taatcatgca 30120 acccaatata accttccgga accacactgc caacaccgct cccccagcca tgcattgaag 30180 tgaaccctgc tgattacaat gacaatgaag aacccaattc tctcgaccgt gaatcacttg 30240 agaatgaaaa atatctatag tggcacaaca tagacataaa tgcatgcatc ttctcataat 30300 ttttaactcc tcaggattta gaaacatatc ccagggaata ggaagctctt gcagaacagt 30360 aaagctggca gaacaaggaa gaccacgaac acaacttaca ctatgcatag tcatagtatc 30420 acaatctggc aacagcgggt ggtcttcagt catagaagct cgggtttcat tttcctcaca 30480 acgtggtaac tgggctctgg tgtaagggtg atgtctggcg catgatgtcg agcgtgcgcg 30540 caaccttgtc ataatggagt tgcttcctga cattctcgta ttttgtatag caaaacgcgg 30600 ccctggcaga acacactctt cttcgccttc tatcctgccg cttagcgtgt tccgtgtgat 30660 agttcaagta caaccacact cttaagttgg tcaaaagaat gctggcttca gttgtaatca 30720 aaactccatc gcatctaatc gttctgagga aatcatccaa gcaatgcaac tggattgtgt 30780 ttcaagcagg agaggagagg gaagagacgg aagaaccatg ttaattttta ttccaaacga 30840 tctcgcagta cttcaaattg tagatcgcgc agatggcatc tctcgccccc actgtgttgg 30900 tgaaaaagca cagctagatc aaaagaaatg cgattttcaa ggtgctcaac ggtggcttcc 30960 agcaaagcct ccacgcgcac atccaagaac aaaagaatac caaaagaagg agcattttct 31020 aactcctcaa tcatcatatt acattcctgc accattccca gataattttc agctttccag 31080 ccttgaatta ttcgtgtcag ttcttgtggt aaatccaatc cacacattac aaacaggtcc 31140 cggagggcgc cctccaccac cattcttaaa cacaccctca taatgacaaa atatcttgct 31200 cctgtgtcac ctgtagcgaa ttgagaatgg caacatcaat tgacatgccc ttggctctaa 31260 gttcttcttt aagttctagt tgtaaaaact ctctcatatt atcaccaaac tgcttagcca 31320 gaagcccccc gggaacaaga gcaggggacg ctacagtgca gtacaagcgc agacctcccc 31380 aattggctcc agcaaaaaca agattggaat aagcatattg ggaaccgcca gtaatatcat 31440 cgaagttgct ggaaatataa tcaggcagag tttcttgtaa aaattgaata aaagaaaaat 31500 ttgccaaaaa aacattcaaa acctctggga tgcaaatgca ataggttacc gcgctgcgct 31560 ccaacattgt tagttttgaa ttagtctgca aaaataaaaa aaaaaacaag cgtcatatca 31620 tagtagcctg acgaacagat ggataaatca gtctttccat cacaagacaa gccacagggt 31680 ctccagctcg accctcgtaa aacctgtcat catgattaaa caacagcacc gaaagttcct 31740 cgcggtgacc agcatgaata attcttgatg aagcatacaa tccagacatg ttagcatcag 31800 ttaacgagaa aaaacagcca acatagcctt tgggtataat tatgcttaat cgtaagtata 31860 gcaaagccac ccctcgcgga tacaaagtaa aaggcacagg agaataaaaa atataattat 31920 ttctctgctg ctgttcaggc aacgtcgccc ccggtccctc taaatacaca tacaaagcct 31980 catcagccat ggcttaccag acaaagtaca gcgggcacac aaagcacaag ctctaaagtg 32040 actctccaac ctctccacaa tatatatata cacaagccct aaactgacgt aatgggagta 32100 aagtgtaaaa aatcccgcca aacccaacac acaccccgaa actgcgtcac cagggaaaag 32160 tacagtttca cttccgcaat cccaacaggc gtaacttcct ctttctcacg gtacgtgata 32220 tcccactaac ttgcaacgtc attttcccac ggtcgcaccg ccccttttag ccgttaaccc 32280 cacagccaat caccacacga tccacacttt ttaaaatcac ctcatttaca tattggcacc 32340 attccatcta taaggtatat tatatagata gaggcgcgcc ctctcttaag gtagcatcgg 32400 gatcgagtcc ctgagagaac atcctcaatc ccgatctatc cttagatccg aggaatatcg 32460 aaatcagtta cgctagggat aacagggtaa tatagcatcc cctcggattg ctatctaccg 32520 gctcgtcagc tatgatctct cgatttcgat caagaaatct cattggttac cttgggctat 32580 cgaaaccagt caagtcagct acttggcgag atcgacttgt ctgagtttcg actacgctca 32640 gaattgcgtc agcgcctatc gccaggtatt actccaatcc cgaatatccg agcctgagag 32700 aacatcctca atcccgatct atccttagat ccgaggaata tcgaaatcgt ttaaatcttt 32760 tcttgatggt aaatcattcg aatataagaa tggagagacg aatggggaaa cgacaaagat 32820 gacattcttt ggtccttctg gtgaggttct caagtttttg gttaatcctg tcaacaactt 32880 atatcgtatg gggctgactt caggtgctac atttgaagag ataaattgca ctgaaatcta 32940 gtaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat 33000 ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc 33060 taccaactct ttgaaccgag gtaactggct tggcagagcg cagtcaccaa aacttgtcct 33120 ttcagtttag ccttaaccgg cgcatgactt caagactaac tctgctaaat caattaccag 33180 tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac 33240 cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc 33300 gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc 33360 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg 33420 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag 33480 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc 33540 tcacttccct gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag 33600 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg 33660 aatatatcct gtatcacata ttctgctgac gcaccggtgc ggcctttttt ctcctgccac 33720 atgaagcact tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc 33780 taatttaaac gtggtgtacc gagaacgatc ctctcagtgc gatctcgacg atcagtggta 33840 ttccgacata tcgttgcttg gcagtcagcc agtcgatcct agcttgggac ccaggaagtc 33900 caatcgtcag atattgtact cagcctggtc acggcagcgt accgatctcg taactataac 33960 ggtcctaagg tagcgaacta gatattgata gtctgatcgg tcaacgtata atcgagtcct 34020 agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca tgattgaaca 34080 agatggattg cacgcaggtt ctccggcggc ttgggtggag aggctattcg gctatgactg 34140 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 34200 tccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 34260 agcgcggcta tcgtggctgg cgacgacggg cgttccttgc gcggctgtgc tcgacgttgt 34320 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 34380 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 34440 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 34500 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 34560 gctcgcgcca gccgaactgt tcgccaggct caaggcgtct atgcccgacg gcgaggatct 34620 cgtcgtgacc cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 34680 tggattcatc gactgtggcc gtctgggtgt ggcggaccgc tatcaggaca tagcgttggc 34740 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc ttgtgcttta 34800 cggtatcgcc gcgcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 34860 ctgaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt 34920 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca 34980 tacgtgtcct ccttaccaga aatttatcct taaggtccgt aactataacg gtcctaaggt 35040 agcgaatcga cctagctcta tcgaatctcc ctcgtttcga gcttacgcga actagcctct 35100 ggcgatagca tccgaggggc aggcatctat gtcgggtgcg gagaaagagg taatgtcaag 35160 ttcgatctga ttgcttggca taaagtccga tggttcgagt agactcagtt caacctctct 35220 cttaaggtag c 35231 <210> 66 <211> 32735 <212> DNA <213> Artificial Sequence <220> <223> pNG-sh01 virus genome sequence consisting of a transgene cassette encoding an shRNA to GAPDH inserted into the region BY <400> 66 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgcccccag tggaaagacg cgcaggcaaa acgcaccacg 29400 tgacggagcg tgaccgcgcg ccgagcccaa ggtcgggcag gaagagggcc tatttcccat 29460 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt 29520 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg 29580 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg 29640 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacggtaaa ccatgtagtt 29700 gaggtcaatg aagggaagct tgccttcatt gacctcaact acatggttta cttttttcct 29760 gcaggaactt gtttatttga aaatcaattc acaaaatccg agtagttatt ttgcctcccc 29820 cttcccattt aacagaatac accaatctct ccccacgcac agctttaaac atttggatac 29880 cattagatat agacatggtt ttagattcca cattccaaac agtttcagag cgagccaatc 29940 tggggtcagt gatagataaa aatccatcgg gatagtcttt taaagcgctt tcacagtcca 30000 actgctgcgg atgcgactcc ggagtctgga tcacggtcat ctggaagaag aacgatggga 30060 atcataatcc gaaaacggta tcggacgatt gtgtctcatc aaacccacaa gcagccgctg 30120 tctgcgtcgc tccgtgcgac tgctgtttat gggatcaggg tccacagtgt cctgaagcat 30180 gattttaata gcccttaaca tcaactttct ggtgcgatgc gcgcagcaac gcattctgat 30240 ttcactcaaa tctttgcagt aggtacaaca cattattaca atattgttta ataaaccata 30300 attaaaagcg ctccagccaa aactcatatc tgatataatc gcccctgcat gaccatcata 30360 ccaaagttta atataaatta aatgacgttc cctcaaaaac acactaccca catacatgat 30420 ctcttttggc atgtgcatat taacaatctg tctgtaccat ggacaacgtt ggttaatcat 30480 gcaacccaat ataaccttcc ggaaccacac tgccaacacc gctcccccag ccatgcattg 30540 aagtgaaccc tgctgattac aatgacaatg aagaacccaa ttctctcgac cgtgaatcac 30600 ttgagaatga aaaatatcta tagtggcaca acatagacat aaatgcatgc atcttctcat 30660 aatttttaac tcctcaggat ttagaaacat atcccaggga ataggaagct cttgcagaac 30720 agtaaagctg gcagaacaag gaagaccacg aacacaactt acactatgca tagtcatagt 30780 atcacaatct ggcaacagcg ggtggtcttc agtcatagaa gctcgggttt cattttcctc 30840 acaacgtggt aactgggctc tggtgtaagg gtgatgtctg gcgcatgatg tcgagcgtgc 30900 gcgcaacctt gtcataatgg agttgcttcc tgacattctc gtattttgta tagcaaaacg 30960 cggccctggc agaacacact cttcttcgcc ttctatcctg ccgcttagcg tgttccgtgt 31020 gatagttcaa gtacaaccac actcttaagt tggtcaaaag aatgctggct tcagttgtaa 31080 tcaaaactcc atcgcatcta atcgttctga ggaaatcatc caagcaatgc aactggattg 31140 tgtttcaagc aggagaggag agggaagaga cggaagaacc atgttaattt ttattccaaa 31200 cgatctcgca gtacttcaaa ttgtagatcg cgcagatggc atctctcgcc cccactgtgt 31260 tggtgaaaaa gcacagctag atcaaaagaa atgcgatttt caaggtgctc aacggtggct 31320 tccagcaaag cctccacgcg cacatccaag aacaaaagaa taccaaaaga aggagcattt 31380 tctaactcct caatcatcat attacattcc tgcaccattc ccagataatt ttcagctttc 31440 cagccttgaa ttattcgtgt cagttcttgt ggtaaatcca atccacacat tacaaacagg 31500 tcccggaggg cgccctccac caccattctt aaacacaccc tcataatgac aaaatatctt 31560 gctcctgtgt cacctgtagc gaattgagaa tggcaacatc aattgacatg cccttggctc 31620 taagttcttc tttaagttct agttgtaaaa actctctcat attatcacca aactgcttag 31680 ccagaagccc cccgggaaca agagcagggg acgctacagt gcagtacaag cgcagacctc 31740 cccaattggc tccagcaaaa acaagattgg aataagcata ttgggaaccg ccagtaatat 31800 catcgaagtt gctggaaata taatcaggca gagtttcttg taaaaattga ataaaagaaa 31860 aatttgccaa aaaaacattc aaaacctctg ggatgcaaat gcaataggtt accgcgctgc 31920 gctccaacat tgttagtttt gaattagtct gcaaaaataa aaaaaaaaac aagcgtcata 31980 tcatagtagc ctgacgaaca gatggataaa tcagtctttc catcacaaga caagccacag 32040 ggtctccagc tcgaccctcg taaaacctgt catcatgatt aaacaacagc accgaaagtt 32100 cctcgcggtg accagcatga ataattcttg atgaagcata caatccagac atgttagcat 32160 cagttaacga gaaaaaacag ccaacatagc ctttgggtat aattatgctt aatcgtaagt 32220 atagcaaagc cacccctcgc ggatacaaag taaaaggcac aggagaataa aaaatataat 32280 tatttctctg ctgctgttca ggcaacgtcg cccccggtcc ctctaaatac acatacaaag 32340 cctcatcagc catggcttac cagacaaagt acagcgggca cacaaagcac aagctctaaa 32400 gtgactctcc aacctctcca caatatatat atacacaagc cctaaactga cgtaatggga 32460 gtaaagtgta aaaaatcccg ccaaacccaa cacacacccc gaaactgcgt caccagggaa 32520 aagtacagtt tcacttccgc aatcccaaca ggcgtaactt cctctttctc acggtacgtg 32580 atatcccact aacttgcaac gtcattttcc cacggtcgca ccgccccttt tagccgttaa 32640 ccccacagcc aatcaccaca cgatccacac tttttaaaat cacctcattt acatattggc 32700 accattccat ctataaggta tattatatag ataga 32735 <210> 67 <211> 643 <212> PRT <213> Artificial Sequence <220> <223> Sodium Iodide symporter (NIS) amino acid sequence <400> 67 Met Glu Ala Val Glu Thr Gly Glu Arg Pro Thr Phe Gly Ala Trp Asp 1 5 10 15 Tyr Gly Val Phe Ala Leu Met Leu Leu Val Ser Thr Gly Ile Gly Leu 20 25 30 Trp Val Gly Leu Ala Arg Gly Gly Gln Arg Ser Ala Glu Asp Phe Phe 35 40 45 Thr Gly Gly Arg Arg Leu Ala Ala Leu Pro Val Gly Leu Ser Leu Ser 50 55 60 Ala Ser Phe Met Ser Ala Val Gln Val Leu Gly Val Pro Ser Glu Ala 65 70 75 80 Tyr Arg Tyr Gly Leu Lys Phe Leu Trp Met Cys Leu Gly Gln Leu Leu 85 90 95 Asn Ser Val Leu Thr Ala Leu Leu Phe Met Pro Val Phe Tyr Arg Leu 100 105 110 Gly Leu Thr Ser Thr Tyr Glu Tyr Leu Glu Met Arg Phe Ser Arg Ala 115 120 125 Val Arg Leu Cys Gly Thr Leu Gln Tyr Ile Val Ala Thr Met Leu Tyr 130 135 140 Thr Gly Ile Val Ile Tyr Ala Pro Ala Leu Ile Leu Asn Gln Val Thr 145 150 155 160 Gly Leu Asp Ile Trp Ala Ser Leu Leu Ser Thr Gly Ile Ile Cys Thr 165 170 175 Phe Tyr Thr Ala Val Gly Gly Met Lys Ala Val Val Trp Thr Asp Val 180 185 190 Phe Gln Val Val Val Met Leu Ser Gly Phe Trp Val Val Leu Ala Arg 195 200 205 Gly Val Met Leu Val Gly Gly Pro Arg Gln Val Leu Thr Leu Ala Gln 210 215 220 Asn His Ser Arg Ile Asn Leu Met Asp Phe Asn Pro Asp Pro Arg Ser 225 230 235 240 Arg Tyr Thr Phe Trp Thr Phe Val Val Gly Gly Thr Leu Val Trp Leu 245 250 255 Ser Met Tyr Gly Val Asn Gln Ala Gln Val Gln Arg Tyr Val Ala Cys 260 265 270 Arg Thr Glu Lys Gln Ala Lys Leu Ala Leu Leu Ile Asn Gln Val Gly 275 280 285 Leu Phe Leu Ile Val Ser Ser Ala Ala Cys Cys Gly Ile Val Met Phe 290 295 300 Val Phe Tyr Thr Asp Cys Asp Pro Leu Leu Leu Gly Arg Ile Ser Ala 305 310 315 320 Pro Asp Gln Tyr Met Pro Leu Leu Val Leu Asp Ile Phe Glu Asp Leu 325 330 335 Pro Gly Val Pro Gly Leu Phe Leu Ala Cys Ala Tyr Ser Gly Thr Leu 340 345 350 Ser Thr Ala Ser Thr Ser Ile Asn Ala Met Ala Ala Val Thr Val Glu 355 360 365 Asp Leu Ile Lys Pro Arg Leu Arg Ser Leu Ala Pro Arg Lys Leu Val 370 375 380 Ile Ile Ser Lys Gly Leu Ser Leu Ile Tyr Gly Ser Ala Cys Leu Thr 385 390 395 400 Val Ala Ala Leu Ser Ser Leu Leu Gly Gly Gly Val Leu Gln Gly Ser 405 410 415 Phe Thr Val Met Gly Val Ile Ser Gly Pro Leu Leu Gly Ala Phe Ile 420 425 430 Leu Gly Met Phe Leu Pro Ala Cys Asn Thr Pro Gly Val Leu Ala Gly 435 440 445 Leu Gly Ala Gly Leu Ala Leu Ser Leu Trp Val Ala Leu Gly Ala Thr 450 455 460 Leu Tyr Pro Pro Ser Glu Gln Thr Met Arg Val Leu Pro Ser Ser Ala 465 470 475 480 Ala Arg Cys Val Ala Leu Ser Val Asn Ala Ser Gly Leu Leu Asp Pro 485 490 495 Ala Leu Leu Pro Ala Asn Asp Ser Ser Arg Ala Pro Ser Ser Gly Met 500 505 510 Asp Ala Ser Arg Pro Ala Leu Ala Asp Ser Phe Tyr Ala Ile Ser Tyr 515 520 525 Leu Tyr Tyr Gly Ala Leu Gly Thr Leu Thr Thr Val Leu Cys Gly Ala 530 535 540 Leu Ile Ser Cys Leu Thr Gly Pro Thr Lys Arg Ser Thr Leu Ala Pro 545 550 555 560 Gly Leu Leu Trp Trp Asp Leu Ala Arg Gln Thr Ala Ser Val Ala Pro 565 570 575 Lys Glu Glu Val Ala Ile Leu Asp Asp Asn Leu Val Lys Gly Pro Glu 580 585 590 Glu Leu Pro Thr Gly Asn Lys Lys Pro Pro Gly Phe Leu Pro Thr Asn 595 600 605 Glu Asp Arg Leu Phe Phe Leu Gly Gln Lys Glu Leu Glu Gly Ala Gly 610 615 620 Ser Trp Thr Pro Cys Val Gly His Asp Gly Gly Arg Asp Gln Gln Glu 625 630 635 640 Thr Asn Leu <210> 68 <211> 34558 <212> DNA <213> Artificial Sequence <220> <223> NG-280 virus genome sequence consisting of a transgene cassette encoding the sodium iodide symporter (NIS) inserted into the region BY <400> 68 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggag gccgtggaga ccggggaacg 29400 gcccaccttc ggagcctggg actacggggt ctttgccctc atgctcctgg tgtccactgg 29460 catcgggctg tgggtcgggc tggctcgggg cgggcagcgc agcgctgagg acttcttcac 29520 cgggggccgg cgcctggcgg ccctgcccgt gggcctgtcg ctgtctgcca gcttcatgtc 29580 ggccgtgcag gtgctgggcg tgccgtcgga ggcctatcgc tatggcctca agttcctctg 29640 gatgtgcctg ggccagcttc tgaactcggt cctcaccgcc ctgctcttca tgcccgtctt 29700 ctaccgcctg ggcctcacca gcacctacga gtacctggag atgcgcttca gccgcgcagt 29760 gcggctctgc gggactttgc agtacattgt agccacgatg ctgtacaccg gcatcgtaat 29820 ctacgcaccg gccctcatcc tgaaccaagt gaccgggctg gacatctggg cgtcgctcct 29880 gtccaccgga attatctgca ccttctacac ggctgtgggc ggcatgaagg ctgtggtctg 29940 gactgatgtg ttccaggtcg tggtgatgct aagtggcttc tgggttgtcc tggcacgcgg 30000 tgtcatgctt gtgggcgggc cccgccaggt gctcacgctg gcccagaacc actcccggat 30060 caacctcatg gactttaacc ctgacccgag gagccgctat acattctgga cttttgtggt 30120 gggtggcacg ttggtgtggc tctccatgta tggcgtgaac caggcgcagg tgcagcgcta 30180 cgtggcttgc cgcacagaga agcaggccaa gctggccctg ctcatcaacc aggtcggcct 30240 gttcctgatc gtgtccagcg ctgcctgctg tggcatcgtc atgtttgtgt tctacactga 30300 ctgcgaccct ctcctcctgg ggcgcatctc tgccccagac cagtacatgc ctctgctggt 30360 gctggacatc ttcgaagatc tgcctggagt ccccgggctt ttcctggcct gtgcttacag 30420 tggcaccctc agcacagcat ccaccagcat caatgctatg gctgcagtca ctgtagaaga 30480 cctcatcaaa cctcggctgc ggagcctggc acccaggaaa ctcgtgatta tctccaaggg 30540 gctctcactc atctacggat cggcctgtct caccgtggca gccctgtcct cactgctcgg 30600 aggaggtgtc cttcagggct ccttcaccgt catgggagtc atcagcggcc ccctgctggg 30660 agccttcatc ttgggaatgt tcctgccggc ctgcaacaca ccgggcgtcc tcgcgggact 30720 aggcgcgggc ttggcgctgt cgctgtgggt ggccttgggc gccacgctgt acccacccag 30780 cgagcagacc atgagggtcc tgccatcgtc ggctgcccgc tgcgtggctc tctcagtcaa 30840 cgcctctggc ctcctggacc cggctctcct ccctgctaac gactccagca gggcccccag 30900 ctcaggaatg gacgccagcc gacccgcctt agctgacagc ttctatgcca tctcctatct 30960 ctattacggt gccctgggca cgctgaccac tgtgctgtgc ggagccctca tcagctgcct 31020 gacaggcccc accaagcgca gcaccctggc cccgggattg ttgtggtggg acctcgcacg 31080 gcagacagca tcagtggccc ccaaggaaga agtggccatc ctggatgaca acttggtcaa 31140 gggtcctgaa gaactcccca ctggaaacaa gaagccccct ggcttcctgc ccaccaatga 31200 ggatcgtctg tttttcttgg ggcagaagga gctggagggg gctggctctt ggaccccctg 31260 tgttggacat gatggtggtc gagaccagca ggagacaaac ctctgagcta gcttgactga 31320 ctgagataca gcgtaccttc agctcacaga catgataaga tacattgatg agtttggaca 31380 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 31440 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 31500 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa 31560 atgtggtagt cgtcagctat cctgcaggaa cttgtttatt tgaaaatcaa ttcacaaaat 31620 ccgagtagtt attttgcctc ccccttccca tttaacagaa tacaccaatc tctccccacg 31680 cacagcttta aacatttgga taccattaga tatagacatg gttttagatt ccacattcca 31740 aacagtttca gagcgagcca atctggggtc agtgatagat aaaaatccat cgggatagtc 31800 ttttaaagcg ctttcacagt ccaactgctg cggatgcgac tccggagtct ggatcacggt 31860 catctggaag aagaacgatg ggaatcataa tccgaaaacg gtatcggacg attgtgtctc 31920 atcaaaccca caagcagccg ctgtctgcgt cgctccgtgc gactgctgtt tatgggatca 31980 gggtccacag tgtcctgaag catgatttta atagccctta acatcaactt tctggtgcga 32040 tgcgcgcagc aacgcattct gatttcactc aaatctttgc agtaggtaca acacattatt 32100 acaatattgt ttaataaacc ataattaaaa gcgctccagc caaaactcat atctgatata 32160 atcgcccctg catgaccatc ataccaaagt ttaatataaa ttaaatgacg ttccctcaaa 32220 aacacactac ccacatacat gatctctttt ggcatgtgca tattaacaat ctgtctgtac 32280 catggacaac gttggttaat catgcaaccc aatataacct tccggaacca cactgccaac 32340 accgctcccc cagccatgca ttgaagtgaa ccctgctgat tacaatgaca atgaagaacc 32400 caattctctc gaccgtgaat cacttgagaa tgaaaaatat ctatagtggc acaacataga 32460 cataaatgca tgcatcttct cataattttt aactcctcag gatttagaaa catatcccag 32520 ggaataggaa gctcttgcag aacagtaaag ctggcagaac aaggaagacc acgaacacaa 32580 cttacactat gcatagtcat agtatcacaa tctggcaaca gcgggtggtc ttcagtcata 32640 gaagctcggg tttcattttc ctcacaacgt ggtaactggg ctctggtgta agggtgatgt 32700 ctggcgcatg atgtcgagcg tgcgcgcaac cttgtcataa tggagttgct tcctgacatt 32760 ctcgtatttt gtatagcaaa acgcggccct ggcagaacac actcttcttc gccttctatc 32820 ctgccgctta gcgtgttccg tgtgatagtt caagtacaac cacactctta agttggtcaa 32880 aagaatgctg gcttcagttg taatcaaaac tccatcgcat ctaatcgttc tgaggaaatc 32940 atccaagcaa tgcaactgga ttgtgtttca agcaggagag gagagggaag agacggaaga 33000 accatgttaa tttttattcc aaacgatctc gcagtacttc aaattgtaga tcgcgcagat 33060 ggcatctctc gcccccactg tgttggtgaa aaagcacagc tagatcaaaa gaaatgcgat 33120 tttcaaggtg ctcaacggtg gcttccagca aagcctccac gcgcacatcc aagaacaaaa 33180 gaataccaaa agaaggagca ttttctaact cctcaatcat catattacat tcctgcacca 33240 ttcccagata attttcagct ttccagcctt gaattattcg tgtcagttct tgtggtaaat 33300 ccaatccaca cattacaaac aggtcccgga gggcgccctc caccaccatt cttaaacaca 33360 ccctcataat gacaaaatat cttgctcctg tgtcacctgt agcgaattga gaatggcaac 33420 atcaattgac atgcccttgg ctctaagttc ttctttaagt tctagttgta aaaactctct 33480 catattatca ccaaactgct tagccagaag ccccccggga acaagagcag gggacgctac 33540 agtgcagtac aagcgcagac ctccccaatt ggctccagca aaaacaagat tggaataagc 33600 atattgggaa ccgccagtaa tatcatcgaa gttgctggaa atataatcag gcagagtttc 33660 ttgtaaaaat tgaataaaag aaaaatttgc caaaaaaaca ttcaaaacct ctgggatgca 33720 aatgcaatag gttaccgcgc tgcgctccaa cattgttagt tttgaattag tctgcaaaaa 33780 taaaaaaaaa aacaagcgtc atatcatagt agcctgacga acagatggat aaatcagtct 33840 ttccatcaca agacaagcca cagggtctcc agctcgaccc tcgtaaaacc tgtcatcatg 33900 attaaacaac agcaccgaaa gttcctcgcg gtgaccagca tgaataattc ttgatgaagc 33960 atacaatcca gacatgttag catcagttaa cgagaaaaaa cagccaacat agcctttggg 34020 tataattatg cttaatcgta agtatagcaa agccacccct cgcggataca aagtaaaagg 34080 cacaggagaa taaaaaatat aattatttct ctgctgctgt tcaggcaacg tcgcccccgg 34140 tccctctaaa tacacataca aagcctcatc agccatggct taccagacaa agtacagcgg 34200 gcacacaaag cacaagctct aaagtgactc tccaacctct ccacaatata tatatacaca 34260 agccctaaac tgacgtaatg ggagtaaagt gtaaaaaatc ccgccaaacc caacacacac 34320 cccgaaactg cgtcaccagg gaaaagtaca gtttcacttc cgcaatccca acaggcgtaa 34380 cttcctcttt ctcacggtac gtgatatccc actaacttgc aacgtcattt tcccacggtc 34440 gcaccgcccc ttttagccgt taaccccaca gccaatcacc acacgatcca cactttttaa 34500 aatcacctca tttacatatt ggcaccattc catctataag gtatattata tagataga 34558 <210> 69 <211> 34327 <212> DNA <213> Artificial Sequence <220> <223> NG-272 virus genome sequence comprising the EnAd genome with a transgene cassette encoding an anti-VEGF ScFv and an anti-PD-L1 ScFv inserted into the region BY. <400> 69 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta cttgaaatca 28200 gcaataaggt ctctgttgaa attttctccc agcagcacct cacttccctc ttcccaactc 28260 tggtattcta aaccccgttc agcggcatac tttctccata ctttaaaggg gatgtcaaat 28320 tttagctcct ctcctgtacc cacaatcttc atgtctttct tcccagatga ccaagagagt 28380 ccggctcagt gactccttca accctgtcta cccctatgaa gatgaaagca cctcccaaca 28440 cccctttata aacccagggt ttatttcccc aaatggcttc acacaaagcc caaacggagt 28500 tcttacttta aaatgtttaa ccccactaac aaccacaggc ggatctctac agctaaaagt 28560 gggaggggga cttacagtgg atgacaccaa cggttttttg aaagaaaaca taagtgccac 28620 cacaccactc gttaagactg gtcactctat aggtttacca ctaggagccg gattgggaac 28680 gaatgaaaat aaactttgta tcaaattagg acaaggactt acattcaatt caaacaacat 28740 ttgcattgat gacaatatta acaccttatg gacaggagtc aaccccaccg aagccaactg 28800 tcaaatcatg aactccagtg aatctaatga ttgcaaatta attctaacac tagttaaaac 28860 tggagcacta gtcactgcat ttgtttatgt tataggagta tctaacaatt ttaatatgct 28920 aactacacac agaaatataa attttactgc agagctgttt ttcgattcta ctggtaattt 28980 actaactaga ctctcatccc tcaaaactcc acttaatcat aaatcaggac aaaacatggc 29040 tactggtgcc attactaatg ctaaaggttt catgcccagc acgactgcct atcctttcaa 29100 tgataattct agagaaaaag aaaactacat ttacggaact tgttactaca cagctagtga 29160 tcgcactgct tttcccattg acatatctgt catgcttaac cgaagagcaa taaatgacga 29220 gacatcatat tgtattcgta taacttggtc ctggaacaca ggagatgccc cagaggtgca 29280 aacctctgct acaaccctag tcacctcccc atttaccttt tactacatca gagaagacga 29340 ctgacaaata aagtttgcga tcgccaggcc caccatggga tggagctgca tcatactgtt 29400 cctggttgcc acagctacag gggtacactc cgaagtccag ctcgttgaaa gcggaggcgg 29460 gttggtccaa cctggcggct cacttcggct ttcctgtgca gctagtgggt tcaccttttc 29520 cgatagctgg atacactggg tgagacaggc tccaggcaaa ggcttggaat gggtcgcctg 29580 gattagcccc tatggaggca gcacctatta cgccgattct gtgaagggga ggttcacaat 29640 ctccgccgat accagtaaga acacagccta tctgcagatg aatagtctcc gagccgagga 29700 cactgcagtg tactactgcg ctaggcgtca ttggccagga ggctttgact actggggtca 29760 aggtaccctc gtgactgtga gtgcaggtgg cggtggctcg ggcggtggtg gatctggtgg 29820 cggcggatct gacatccaga tgacacagag ccctagctca ctgtctgcga gcgtaggcga 29880 cagagtgacc atcacttgtc gggcttccca ggatgtgtcc acagccgttg catggtacca 29940 gcagaaaccc gggaaagcac caaagctgct gatctacagc gcttctttcc tgtactcagg 30000 cgtgcctagc cgctttagtg ggtctggttc tgggactgac ttcaccctca cgatttcctc 30060 actgcaaccc gaggattttg cgacctatta ctgccagcag tatctgtatc atccggccac 30120 ttttgggcaa ggcacgaagg tcgagattaa gcgccatcat caccaccacc acggaagcgg 30180 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgg 30240 ttggtcgtgt ataatcctat ttctcgtagc aactgcgact ggcgtccata gtgaggttca 30300 gctggtggag tctggcggtg gcctggtgca gccagggggt tcactccgtt tgtcttgtgc 30360 ggcttctggc tataccttca ccaactatgg tatgaactgg gtccgtcagg ccccgggtaa 30420 gggcctggag tgggttggat ggattaacac ctataccggt gaaccgacct atgctgcgga 30480 tttcaaacgt cgttttactt tttctctgga cacctccaag agcacagctt acctccagat 30540 gaacagcctg cgcgctgagg acactgccgt ctattactgt gcaaagtacc cgcactatta 30600 tgggagcagc cactggtatt tcgacgtctg gggacaagga accctggtca ccgtctcctc 30660 gggaggtggt ggaagtgggg gcggcggtag cggagggggt ggtagcgata tacaaatgac 30720 ccagtccccg tctagcctgt ccgcctctgt gggcgatagg gtcaccatca cctgcagcgc 30780 aagtcaggat attagcaact atttaaactg gtatcaacag aaaccaggaa aagctccgaa 30840 agtcctgatt tacttcacct cctctctcca ctctggagtc ccttctcgct tctctggatc 30900 cggaagtggg acggatttca ctctgaccat cagcagtctg cagccagaag acttcgcaac 30960 ttattactgt caacagtata gcaccgtgcc gtggacgttt ggacagggta ccaaagtgga 31020 aataaaacga ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtaagctag 31080 cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat acattgatga 31140 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga 31200 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg 31260 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa 31320 cctctacaaa tgtggtagtc gtcagctatc ctgcaggaac ttgtttattt gaaaatcaat 31380 tcacaaaatc cgagtagtta ttttgcctcc cccttcccat ttaacagaat acaccaatct 31440 ctccccacgc acagctttaa acatttggat accattagat atagacatgg ttttagattc 31500 cacattccaa acagtttcag agcgagccaa tctggggtca gtgatagata aaaatccatc 31560 gggatagtct tttaaagcgc tttcacagtc caactgctgc ggatgcgact ccggagtctg 31620 gatcacggtc atctggaaga agaacgatgg gaatcataat ccgaaaacgg tatcggacga 31680 ttgtgtctca tcaaacccac aagcagccgc tgtctgcgtc gctccgtgcg actgctgttt 31740 atgggatcag ggtccacagt gtcctgaagc atgattttaa tagcccttaa catcaacttt 31800 ctggtgcgat gcgcgcagca acgcattctg atttcactca aatctttgca gtaggtacaa 31860 cacattatta caatattgtt taataaacca taattaaaag cgctccagcc aaaactcata 31920 tctgatataa tcgcccctgc atgaccatca taccaaagtt taatataaat taaatgacgt 31980 tccctcaaaa acacactacc cacatacatg atctcttttg gcatgtgcat attaacaatc 32040 tgtctgtacc atggacaacg ttggttaatc atgcaaccca atataacctt ccggaaccac 32100 actgccaaca ccgctccccc agccatgcat tgaagtgaac cctgctgatt acaatgacaa 32160 tgaagaaccc aattctctcg accgtgaatc acttgagaat gaaaaatatc tatagtggca 32220 caacatagac ataaatgcat gcatcttctc ataattttta actcctcagg atttagaaac 32280 atatcccagg gaataggaag ctcttgcaga acagtaaagc tggcagaaca aggaagacca 32340 cgaacacaac ttacactatg catagtcata gtatcacaat ctggcaacag cgggtggtct 32400 tcagtcatag aagctcgggt ttcattttcc tcacaacgtg gtaactgggc tctggtgtaa 32460 gggtgatgtc tggcgcatga tgtcgagcgt gcgcgcaacc ttgtcataat ggagttgctt 32520 cctgacattc tcgtattttg tatagcaaaa cgcggccctg gcagaacaca ctcttcttcg 32580 ccttctatcc tgccgcttag cgtgttccgt gtgatagttc aagtacaacc acactcttaa 32640 gttggtcaaa agaatgctgg cttcagttgt aatcaaaact ccatcgcatc taatcgttct 32700 gaggaaatca tccaagcaat gcaactggat tgtgtttcaa gcaggagagg agagggaaga 32760 gacggaagaa ccatgttaat ttttattcca aacgatctcg cagtacttca aattgtagat 32820 cgcgcagatg gcatctctcg cccccactgt gttggtgaaa aagcacagct agatcaaaag 32880 aaatgcgatt ttcaaggtgc tcaacggtgg cttccagcaa agcctccacg cgcacatcca 32940 agaacaaaag aataccaaaa gaaggagcat tttctaactc ctcaatcatc atattacatt 33000 cctgcaccat tcccagataa ttttcagctt tccagccttg aattattcgt gtcagttctt 33060 gtggtaaatc caatccacac attacaaaca ggtcccggag ggcgccctcc accaccattc 33120 ttaaacacac cctcataatg acaaaatatc ttgctcctgt gtcacctgta gcgaattgag 33180 aatggcaaca tcaattgaca tgcccttggc tctaagttct tctttaagtt ctagttgtaa 33240 aaactctctc atattatcac caaactgctt agccagaagc cccccgggaa caagagcagg 33300 ggacgctaca gtgcagtaca agcgcagacc tccccaattg gctccagcaa aaacaagatt 33360 ggaataagca tattgggaac cgccagtaat atcatcgaag ttgctggaaa tataatcagg 33420 cagagtttct tgtaaaaatt gaataaaaga aaaatttgcc aaaaaaacat tcaaaacctc 33480 tgggatgcaa atgcaatagg ttaccgcgct gcgctccaac attgttagtt ttgaattagt 33540 ctgcaaaaat aaaaaaaaaa acaagcgtca tatcatagta gcctgacgaa cagatggata 33600 aatcagtctt tccatcacaa gacaagccac agggtctcca gctcgaccct cgtaaaacct 33660 gtcatcatga ttaaacaaca gcaccgaaag ttcctcgcgg tgaccagcat gaataattct 33720 tgatgaagca tacaatccag acatgttagc atcagttaac gagaaaaaac agccaacata 33780 gcctttgggt ataattatgc ttaatcgtaa gtatagcaaa gccacccctc gcggatacaa 33840 agtaaaaggc acaggagaat aaaaaatata attatttctc tgctgctgtt caggcaacgt 33900 cgcccccggt ccctctaaat acacatacaa agcctcatca gccatggctt accagacaaa 33960 gtacagcggg cacacaaagc acaagctcta aagtgactct ccaacctctc cacaatatat 34020 atatacacaa gccctaaact gacgtaatgg gagtaaagtg taaaaaatcc cgccaaaccc 34080 aacacacacc ccgaaactgc gtcaccaggg aaaagtacag tttcacttcc gcaatcccaa 34140 caggcgtaac ttcctctttc tcacggtacg tgatatccca ctaacttgca acgtcatttt 34200 cccacggtcg caccgcccct tttagccgtt aaccccacag ccaatcacca cacgatccac 34260 actttttaaa atcacctcat ttacatattg gcaccattcc atctataagg tatattatat 34320 agataga 34327 <210> 70 <211> 125 <212> PRT <213> Artificial Sequence <220> <223> anti-CTLA-4 VH chain amino acid sequence. <400> 70 Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30 Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val 50 55 60 Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Asp Pro Arg Gly Ala Thr Leu Tyr Tyr Tyr Tyr Tyr Gly Met 100 105 110 Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 115 120 125 <210> 71 <211> 108 <212> PRT <213> Artificial Sequence <220> <223> anti-CTLA-4 VL chain amino acid sequence <400> 71 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Asn Ser Tyr 20 25 30 Leu Asp Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Thr Pro Phe 85 90 95 Thr Phe Gly Pro Gly Thr Lys Val Glu Ile Lys Arg 100 105 <210> 72 <211> 33464 <212> DNA <213> Artificial Sequence <220> <223> NG-257 virus genome sequence comprising the EnAd genome with a transgene cassette encoding an anti-VEGF ScFv inserted into the region Bx <400> 72 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta ctgcggccgc 28200 tgctaatcct ttctctcttc aggccaccat gggatggagc tgtatcatcc tcttcttggt 28260 agcaacagct acaggtgtcc actccgaggt tcagctggtg gagtctggcg gtggcctggt 28320 gcagccaggg ggctcactcc gtttgtcctg tgcagcttct ggctatacct tcaccaacta 28380 tggtatgaac tgggtccgtc aggccccggg taagggcctg gaatgggttg gatggattaa 28440 cacctatacc ggtgaaccga cctatgctgc ggatttcaaa cgtcgtttta ctttttctct 28500 ggacacctcc aagagcacag cttacctgca gatgaacagc ctgcgcgctg aggacactgc 28560 cgtctattac tgtgcaaagt acccgcacta ttatgggagc agccactggt atttcgacgt 28620 ctggggtcaa ggaaccctgg tcaccgtctc ctcgggtggc ggtggctcgg gcggtggtgg 28680 atctggtggc ggcggatctg atatccagat gacccagtcc ccgagctccc tgtccgcctc 28740 tgtgggcgat agggtcacca tcacctgcag cgcaagtcag gatattagca actatttaaa 28800 ctggtatcaa cagaaaccag gaaaagctcc gaaagtcctg atttacttca cctcctctct 28860 ccactctgga gtcccttctc gcttctctgg atccggttct gggacggatt tcactctgac 28920 catcagcagt ctgcagccag aagacttcgc aacttattac tgtcaacagt atagcaccgt 28980 gccgtggacg tttggacagg gtaccaaggt ggagatcaaa cgacatcatc accaccacca 29040 ttaagctagc ttgactgact gagatacagc gtaccttcag ctcacagaca tgataagata 29100 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 29160 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 29220 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 29280 caagtaaaac ctctacaaat gtgggccggc ctgaaatcag caataaggtc tctgttgaaa 29340 ttttctccca gcagcacctc acttccctct tcccaactct ggtattctaa accccgttca 29400 gcggcatact ttctccatac tttaaagggg atgtcaaatt ttagctcctc tcctgtaccc 29460 acaatcttca tgtctttctt cccagatgac caagagagtc cggctcagtg actccttcaa 29520 ccctgtctac ccctatgaag atgaaagcac ctcccaacac ccctttataa acccagggtt 29580 tatttcccca aatggcttca cacaaagccc aaacggagtt cttactttaa aatgtttaac 29640 cccactaaca accacaggcg gatctctaca gctaaaagtg ggagggggac ttacagtgga 29700 tgacaccaac ggttttttga aagaaaacat aagtgccacc acaccactcg ttaagactgg 29760 tcactctata ggtttaccac taggagccgg attgggaacg aatgaaaata aactttgtat 29820 caaattagga caaggactta cattcaattc aaacaacatt tgcattgatg acaatattaa 29880 caccttatgg acaggagtca accccaccga agccaactgt caaatcatga actccagtga 29940 atctaatgat tgcaaattaa ttctaacact agttaaaact ggagcactag tcactgcatt 30000 tgtttatgtt ataggagtat ctaacaattt taatatgcta actacacaca gaaatataaa 30060 ttttactgca gagctgtttt tcgattctac tggtaattta ctaactagac tctcatccct 30120 caaaactcca cttaatcata aatcaggaca aaacatggct actggtgcca ttactaatgc 30180 taaaggtttc atgcccagca cgactgccta tcctttcaat gataattcta gagaaaaaga 30240 aaactacatt tacggaactt gttactacac agctagtgat cgcactgctt ttcccattga 30300 catatctgtc atgcttaacc gaagagcaat aaatgacgag acatcatatt gtattcgtat 30360 aacttggtcc tggaacacag gagatgcccc agaggtgcaa acctctgcta caaccctagt 30420 cacctcccca tttacctttt actacatcag agaagacgac tgacaaataa agtttgcgat 30480 cgctaccctg caggaacttg tttatttgaa aatcaattca caaaatccga gtagttattt 30540 tgcctccccc ttcccattta acagaataca ccaatctctc cccacgcaca gctttaaaca 30600 tttggatacc attagatata gacatggttt tagattccac attccaaaca gtttcagagc 30660 gagccaatct ggggtcagtg atagataaaa atccatcggg atagtctttt aaagcgcttt 30720 cacagtccaa ctgctgcgga tgcgactccg gagtctggat cacggtcatc tggaagaaga 30780 acgatgggaa tcataatccg aaaacggtat cggacgattg tgtctcatca aacccacaag 30840 cagccgctgt ctgcgtcgct ccgtgcgact gctgtttatg ggatcagggt ccacagtgtc 30900 ctgaagcatg attttaatag cccttaacat caactttctg gtgcgatgcg cgcagcaacg 30960 cattctgatt tcactcaaat ctttgcagta ggtacaacac attattacaa tattgtttaa 31020 taaaccataa ttaaaagcgc tccagccaaa actcatatct gatataatcg cccctgcatg 31080 accatcatac caaagtttaa tataaattaa atgacgttcc ctcaaaaaca cactacccac 31140 atacatgatc tcttttggca tgtgcatatt aacaatctgt ctgtaccatg gacaacgttg 31200 gttaatcatg caacccaata taaccttccg gaaccacact gccaacaccg ctcccccagc 31260 catgcattga agtgaaccct gctgattaca atgacaatga agaacccaat tctctcgacc 31320 gtgaatcact tgagaatgaa aaatatctat agtggcacaa catagacata aatgcatgca 31380 tcttctcata atttttaact cctcaggatt tagaaacata tcccagggaa taggaagctc 31440 ttgcagaaca gtaaagctgg cagaacaagg aagaccacga acacaactta cactatgcat 31500 agtcatagta tcacaatctg gcaacagcgg gtggtcttca gtcatagaag ctcgggtttc 31560 attttcctca caacgtggta actgggctct ggtgtaaggg tgatgtctgg cgcatgatgt 31620 cgagcgtgcg cgcaaccttg tcataatgga gttgcttcct gacattctcg tattttgtat 31680 agcaaaacgc ggccctggca gaacacactc ttcttcgcct tctatcctgc cgcttagcgt 31740 gttccgtgtg atagttcaag tacaaccaca ctcttaagtt ggtcaaaaga atgctggctt 31800 cagttgtaat caaaactcca tcgcatctaa tcgttctgag gaaatcatcc aagcaatgca 31860 actggattgt gtttcaagca ggagaggaga gggaagagac ggaagaacca tgttaatttt 31920 tattccaaac gatctcgcag tacttcaaat tgtagatcgc gcagatggca tctctcgccc 31980 ccactgtgtt ggtgaaaaag cacagctaga tcaaaagaaa tgcgattttc aaggtgctca 32040 acggtggctt ccagcaaagc ctccacgcgc acatccaaga acaaaagaat accaaaagaa 32100 ggagcatttt ctaactcctc aatcatcata ttacattcct gcaccattcc cagataattt 32160 tcagctttcc agccttgaat tattcgtgtc agttcttgtg gtaaatccaa tccacacatt 32220 acaaacaggt cccggagggc gccctccacc accattctta aacacaccct cataatgaca 32280 aaatatcttg ctcctgtgtc acctgtagcg aattgagaat ggcaacatca attgacatgc 32340 ccttggctct aagttcttct ttaagttcta gttgtaaaaa ctctctcata ttatcaccaa 32400 actgcttagc cagaagcccc ccgggaacaa gagcagggga cgctacagtg cagtacaagc 32460 gcagacctcc ccaattggct ccagcaaaaa caagattgga ataagcatat tgggaaccgc 32520 cagtaatatc atcgaagttg ctggaaatat aatcaggcag agtttcttgt aaaaattgaa 32580 taaaagaaaa atttgccaaa aaaacattca aaacctctgg gatgcaaatg caataggtta 32640 ccgcgctgcg ctccaacatt gttagttttg aattagtctg caaaaataaa aaaaaaaaca 32700 agcgtcatat catagtagcc tgacgaacag atggataaat cagtctttcc atcacaagac 32760 aagccacagg gtctccagct cgaccctcgt aaaacctgtc atcatgatta aacaacagca 32820 ccgaaagttc ctcgcggtga ccagcatgaa taattcttga tgaagcatac aatccagaca 32880 tgttagcatc agttaacgag aaaaaacagc caacatagcc tttgggtata attatgctta 32940 atcgtaagta tagcaaagcc acccctcgcg gatacaaagt aaaaggcaca ggagaataaa 33000 aaatataatt atttctctgc tgctgttcag gcaacgtcgc ccccggtccc tctaaataca 33060 catacaaagc ctcatcagcc atggcttacc agacaaagta cagcgggcac acaaagcaca 33120 agctctaaag tgactctcca acctctccac aatatatata tacacaagcc ctaaactgac 33180 gtaatgggag taaagtgtaa aaaatcccgc caaacccaac acacaccccg aaactgcgtc 33240 accagggaaa agtacagttt cacttccgca atcccaacag gcgtaacttc ctctttctca 33300 cggtacgtga tatcccacta acttgcaacg tcattttccc acggtcgcac cgcccctttt 33360 agccgttaac cccacagcca atcaccacac gatccacact ttttaaaatc acctcattta 33420 catattggca ccattccatc tataaggtat attatataga taga 33464 <210> 73 <211> 33464 <212> DNA <213> Artificial Sequence <220> <223> NG-281 virus genome sequence comprising the EnAd genome with a transgene cassette encoding an anti-VEGF ScFv inserted into the region Bx and a second transgene cassette encoding an anti-PD-L1 ScFv inserted into the region BY. <400> 73 tctatctata taatatacct tatagatgga atggtgccaa tatgtaaatg aggtgatttt 60 aaaaagtgtg gatcgtgtgg tgattggctg tggggttaac ggctaaaagg ggcggtgcga 120 ccgtgggaaa atgacgtttt gtgggggtgg agtttttttg caagttgtcg cgggaaatgt 180 gacgcataaa aaggcttttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgttg attttcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgta aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aagggttaga attagatccg cctttggaca cttttgatac 840 tccaggggta attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagaacgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtgtg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgaatcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacgtc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaca gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgtg tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt aggaagacta ggcaactgtt agagaacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaacaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcag gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgct gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt catagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtt cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctggg gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattt caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttgattaag ggaaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgctggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgatga cgaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tttttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tacgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct cagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 tggggtggga ttttcagatg gacagattga gtaaaaattt gttttttctg tcttgcagct 3480 gtcatgagtg gaaacgcttc ttttaagggg ggagtcttca gcccttatct gacagggcgt 3540 ctcccatcct gggcaggagt tcgtcagaat gttatgggat ctactgtgga tggaagaccc 3600 gtccaacccg ccaattcttc aacgctgacc tatgctactt taagttcttc acctttggac 3660 gcagctgcag ctgccgccgc cgcttctgtt gccgctaaca ctgtgcttgg aatgggttac 3720 tatggaagca tcatggctaa ttccacttcc tctaataacc cttctaccct gactcaggac 3780 aagttacttg tccttttggc ccagctggag gctttgaccc aacgtctggg tgaactttct 3840 cagcaggtgg tcgagttgcg agtacaaact gagtctgctg tcggcacggc aaagtctaaa 3900 taaaaaaatc ccagaatcaa tgaataaata aacaagcttg ttgttgattt aaaatcaagt 3960 gtttttattt catttttcgc gcacggtatg ccctagacca ccgatctcta tcattgagaa 4020 ctcggtggat tttttccagg atcctataga ggtgggattg aatgtttaga tacatgggca 4080 ttaggccgtc tttggggtgg agatagctcc attgaaggga ttcatgctcc ggggtagtgt 4140 tgtaaatcac ccagtcataa caaggtcgca gtgcatggtg ttgcacaata tcttttagaa 4200 gtaggctgat tgccacagat aagcccttgg tgtaggtgtt tacaaaccgg ttgagctggg 4260 atgggtgcat tcggggtgaa attatgtgca ttttggattg gatttttaag ttggcaatat 4320 tgccgccaag atcccgtctt gggttcatgt tatgaaggac caccaagacg gtgtatccgg 4380 tacatttagg aaatttatcg tgcagcttgg atggaaaagc gtggaaaaat ttggagacac 4440 ccttgtgtcc tccaagattt tccatgcact catccatgat aatagcaatg gggccgtggg 4500 cagcggcgcg ggcaaacacg ttccgtgggt ctgacacatc atagttatgt tcctgagtta 4560 aatcatcata agccatttta atgaatttgg ggcggagagt accagattgg ggtatgaatg 4620 ttccttcggg ccccggagca tagttcccct cacagatttg catttcccaa gctttcagtt 4680 ccgagggtgg aatcatgtcc acctgggggg ctatgaaaaa caccgtttct ggggcggggg 4740 tgattaattg tgatgatagc aaatttctga gcaattgaga tttgccacat ccggtggggc 4800 cataaatgat tccgattacg ggttgcaggt ggtagtttag ggaacggcaa ctgccgtctt 4860 ctcgaagcaa gggggccacc tcgttcatca tttcccttac atgcatattt tcccgcacca 4920 aatccattag gaggcgctct cctcctagtg atagaagttc ttgtagtgag gaaaagtttt 4980 tcagcggttt cagaccgtca gccatgggca ttttggagag agtttgctgc aaaagttcta 5040 gtctgttcca cagttcagtg atgtgttcta tggcatctcg atccagcaga cctcctcgtt 5100 tcgcgggttt ggacggctcc tggaataggg tatgagacga tgggcgtcca gcgctgccag 5160 ggttcggtcc ttccagggtc tcagtgttcg agtcagggtt gtttccgtca cagtgaaggg 5220 gtgtgcgcct gcttgggcgc ttgccagggt gcgcttcaga ctcatcctgc tggtcgaaaa 5280 cttctgtcgc ttggcgccct gtatgtcggc caagtagcag tttaccatga gttcgtagtt 5340 gagcgcctcg gctgcgtggc ctttggcgcg gagcttacct ttggaagttt tcttgcatac 5400 cgggcagtat aggcatttca gcgcatacaa cttgggcgca aggaaaacgg attctgggga 5460 gtatgcatct gcgccgcagg aggcgcaaac agtttcacat tccaccagcc aggttaaatc 5520 cggttcattg gggtcaaaaa caagttttcc gccatatttt ttgatgcgtt tcttaccttt 5580 ggtctccatg agttcgtgtc ctcgttgagt gacaaacagg ctgtccgtgt ccccgtagac 5640 tgattttaca ggcctcttct ccagtggagt gcctcggtct tcttcgtaca ggaactctga 5700 ccactctgat acaaaggcgc gcgtccaggc cagcacaaag gaggctatgt gggaggggta 5760 gcgatcgttg tcaaccaggg ggtccacctt ttccaaagta tgcaaacaca tgtcaccctc 5820 ttcaacatcc aggaatgtga ttggcttgta ggtgtatttc acgtgacctg gggtccccgc 5880 tgggggggta taaaaggggg cggttctttg ctcttcctca ctgtcttccg gatcgctgtc 5940 caggaacgtc agctgttggg gtaggtattc cctctcgaag gcgggcatga cctctgcact 6000 caggttgtca gtttctaaga acgaggagga tttgatattg acagtgccgg ttgagatgcc 6060 tttcatgagg ttttcgtcca tctggtcaga aaacacaatt tttttattgt caagtttggt 6120 ggcaaatgat ccatacaggg cgttggataa aagtttggca atggatcgca tggtttggtt 6180 cttttccttg tccgcgcgct ctttggcggc gatgttgagt tggacatact cgcgtgccag 6240 gcacttccat tcggggaaga tagttgttaa ttcatctggc acgattctca cttgccaccc 6300 tcgattatgc aaggtaatta aatccacact ggtggccacc tcgcctcgaa ggggttcatt 6360 ggtccaacag agcctacctc ctttcctaga acagaaaggg ggaagtgggt ctagcataag 6420 ttcatcggga gggtctgcat ccatggtaaa gattcccgga agtaaatcct tatcaaaata 6480 gctgatggga gtggggtcat ctaaggccat ttgccattct cgagctgcca gtgcgcgctc 6540 atatgggtta aggggactgc cccatggcat gggatgggtg agtgcagagg catacatgcc 6600 acagatgtca tagacgtaga tgggatcctc aaagatgcct atgtaggttg gatagcatcg 6660 cccccctctg atacttgctc gcacatagtc atatagttca tgtgatggcg ctagcagccc 6720 cggacccaag ttggtgcgat tgggtttttc tgttctgtag acgatctggc gaaagatggc 6780 gtgagaattg gaagagatgg tgggtctttg aaaaatgttg aaatgggcat gaggtagacc 6840 tacagagtct ctgacaaagt gggcataaga ttcttgaagc ttggttacca gttcggcggt 6900 gacaagtacg tctagggcgc agtagtcaag tgtttcttga atgatgtcat aacctggttg 6960 gtttttcttt tcccacagtt cgcggttgag aaggtattct tcgcgatcct tccagtactc 7020 ttctagcgga aacccgtctt tgtctgcacg gtaagatcct agcatgtaga actgattaac 7080 tgccttgtaa gggcagcagc ccttctctac gggtagagag tatgcttgag cagcttttcg 7140 tagcgaagcg tgagtaaggg caaaggtgtc tctgaccatg actttgagga attggtattt 7200 gaagtcgatg tcgtcacagg ctccctgttc ccagagttgg aagtctaccc gtttcttgta 7260 ggcggggttg ggcaaagcga aagtaacatc attgaagaga atcttgccgg ccctgggcat 7320 gaaattgcga gtgatgcgaa aaggctgtgg tacttccgct cggttattga taacctgggc 7380 agctaggacg atctcgtcga aaccgttgat gttgtgtcct acgatgtata attctatgaa 7440 acgcggcgtg cctctgacgt gaggtagctt actgagctca tcaaaggtta ggtctgtggg 7500 gtcagataag gcgtagtgtt cgagagccca ttcgtgcagg tgaggattcg ctttaaggaa 7560 ggaggaccag aggtccactg ccagtgctgt ttgtaactgg tcccggtact gacgaaaatg 7620 ccgtccgact gccatttttt ctggggtgac gcaatagaag gtttgggggt cctgccgcca 7680 gcgatcccac ttgagtttta tggcgaggtc ataggcgatg ttgacgagcc gctggtctcc 7740 agagagtttc atgaccagca tgaaggggat tagctgcttg ccaaaggacc ccatccaggt 7800 gtaggtttcc acatcgtagg tgagaaagag cctttctgtg cgaggatgag agccaatcgg 7860 gaagaactgg atctcctgcc accagttgga ggaatggctg ttgatgtgat ggaagtagaa 7920 ctccctgcga cgcgccgagc attcatgctt gtgcttgtac agacggccgc agtagtcgca 7980 gcgttgcacg ggttgtatct cgtgaatgag ttgtacctgg cttcccttga cgagaaattt 8040 cagtgggaag ccgaggcctg gcgattgtat ctcgtgcttt actatgttgt ctgcatcggc 8100 ctgttcatct tctgtctcga tggtggtcat gctgacgagc cctcgcggga ggcaagtcca 8160 gacctcggcg cggcaggggc ggagctcgag gacgagagcg cgcaggctgg agctgtccag 8220 ggtcctgaga cgctgcggac tcaggttagt aggcagtgtc aggagattaa cttgcatgat 8280 cttttggagg gcgtgcggga ggttcagata gtacttgatc tcaacgggtc cgttggtgga 8340 gatgtcgatg gcttgcaggg ttccgtgtcc cttgggcgct accaccgtgc ccttgttttt 8400 cattttggac ggcggtggct ctgttgcttc ttgcatgttt agaagcggtg tcgagggcgc 8460 gcaccgggcg gcaggggcgg ctcgggaccc ggcggcatgg ctggcagtgg tacgtcggcg 8520 ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac tcgcatgcgc gacgacgcgg 8580 cggttgacat cctggatctg acgcctctgg gtgaaagcta ccggccccgt gagcttgaac 8640 ctgaaagaga gttcaacaga atcaatctcg gtatcgttga cggcggcttg cctaaggatt 8700 tcttgcacgt caccagagtt gtcctggtag gcgatctccg ccatgaactg ctcgatctct 8760 tcctcttgaa gatctccgcg gcccgctctc tcgacggtgg ccgcgaggtc gttggagatg 8820 cgcccaatga gttgagagaa tgcattcatg cccgcctcgt tccagacgcg gctgtagacc 8880 acggccccca cgggatctct cgcgcgcatg accacctggg cgaggttgag ctccacgtgg 8940 cgggtgaaga ccgcatagtt gcataggcgc tggaaaaggt agttgagtgt ggtggcgatg 9000 tgctcggtga cgaagaaata catgatccat cgtctcagcg gcatctcgct gacatcgccc 9060 agagcttcca agcgctccat ggcctcgtag aagtccacgg caaaattaaa aaactgggag 9120 tttcgcgcgg acacggtcaa ctcctcttcc agaagacgga taagttcggc gatggtggtg 9180 cgcacctcgc gctcgaaagc ccctgggatt tcttcctcaa tctcttcttc ttccactaac 9240 atctcttcct cttcaggtgg ggctgcagga ggagggggaa cgcggcgacg ccggcggcgc 9300 acgggcagac ggtcgatgaa tctttcaatg acctctccgc ggcggcggcg catggtttca 9360 gtgacggcgc ggccgttctc gcgcggtcgc agagtaaaaa caccgccgcg catctcctta 9420 aagtggtgac tgggaggttc tccgtttggg agggagaggg cgctgattat acattttatt 9480 aattggcccg tagggactgc acgcagagat ctgatcgtgt caagatccac gggatctgaa 9540 aacctttcga cgaaagcgtc taaccagtca cagtcacaag gtaggctgag tacggcttct 9600 tgtgggcggg ggtggttatg tgttcggtct gggtcttctg tttcttcttc atctcgggaa 9660 ggtgagacga tgctgctggt gatgaaatta aagtaggcag ttctaagacg gcggatggtg 9720 gcgaggagca ccaggtcttt gggtccggct tgctggatac gcaggcgatt ggccattccc 9780 caagcattat cctgacatct agcaagatct ttgtagtagt cttgcatgag ccgttctacg 9840 ggcacttctt cctcacccgt tctgccatgc atacgtgtga gtccaaatcc gcgcattggt 9900 tgtaccagtg ccaagtcagc tacgactctt tcggcgagga tggcttgctg tacttgggta 9960 agggtggctt gaaagtcatc aaaatccaca aagcggtggt aagctcctgt attaatggtg 10020 taagcacagt tggccatgac tgaccagtta actgtctggt gaccagggcg cacgagctcg 10080 gtgtatttaa ggcgcgaata ggcgcgggtg tcaaagatgt aatcgttgca ggtgcgcacc 10140 agatactggt accctataag aaaatgcggc ggtggttggc ggtagagagg ccatcgttct 10200 gtagctggag cgccaggggc gaggtcttcc aacataaggc ggtgatagcc gtagatgtac 10260 ctggacatcc aggtgattcc tgcggcggta gtagaagccc gaggaaactc gcgtacgcgg 10320 ttccaaatgt tgcgtagcgg catgaagtag ttcattgtag gcacggtttg accagtgagg 10380 cgcgcgcagt cattgatgct ctatagacac ggagaaaatg aaagcgttca gcgactcgac 10440 tccgtagcct ggaggaacgt gaacgggttg ggtcgcggtg taccccggtt cgagacttgt 10500 actcgagccg gccggagccg cggctaacgt ggtattggca ctcccgtctc gacccagcct 10560 acaaaaatcc aggatacgga atcgagtcgt tttgctggtt tccgaatggc agggaagtga 10620 gtcctatttt ttttttttgc cgctcagatg catcccgtgc tgcgacagat gcgcccccaa 10680 caacagcccc cctcgcagca gcagcagcag caatcacaaa aggctgtccc tgcaactact 10740 gcaactgccg ccgtgagcgg tgcgggacag cccgcctatg atctggactt ggaagagggc 10800 gaaggactgg cacgtctagg tgcgccttca cccgagcggc atccgcgagt tcaactgaaa 10860 aaagattctc gcgaggcgta tgtgccccaa cagaacctat ttagagacag aagcggcgag 10920 gagccggagg agatgcgagc ttcccgcttt aacgcgggtc gtgagctgcg tcacggtttg 10980 gaccgaagac gagtgttgcg ggacgaggat ttcgaagttg atgaaatgac agggatcagt 11040 cctgccaggg cacacgtggc tgcagccaac cttgtatcgg cttacgagca gacagtaaag 11100 gaagagcgta acttccaaaa gtcttttaat aatcatgtgc gaaccctgat tgcccgcgaa 11160 gaagttaccc ttggtttgat gcatttgtgg gatttgatgg aagctatcat tcagaaccct 11220 actagcaaac ctctgaccgc ccagctgttt ctggtggtgc aacacagcag agacaatgag 11280 gctttcagag aggcgctgct gaacatcacc gaacccgagg ggagatggtt gtatgatctt 11340 atcaacattc tacagagtat catagtgcag gagcggagcc tgggcctggc cgagaaggtg 11400 gctgccatca attactcggt tttgagcttg ggaaaatatt acgctcgcaa aatctacaag 11460 actccatacg ttcccataga caaggaggtg aagatagatg ggttctacat gcgcatgacg 11520 ctcaaggtct tgaccctgag cgatgatctt ggggtgtatc gcaatgacag aatgcatcgc 11580 gcggttagcg ccagcaggag gcgcgagtta agcgacaggg aactgatgca cagtttgcaa 11640 agagctctga ctggagctgg aaccgagggt gagaattact tcgacatggg agctgacttg 11700 cagtggcagc ctagtcgcag ggctctgagc gccgcgacgg caggatgtga gcttccttac 11760 atagaagagg cggatgaagg cgaggaggaa gagggcgagt acttggaaga ctgatggcac 11820 aacccgtgtt ttttgctaga tggaacagca agcaccggat cccgcaatgc gggcggcgct 11880 gcagagccag ccgtccggca ttaactcctc ggacgattgg acccaggcca tgcaacgtat 11940 catggcgttg acgactcgca accccgaagc ctttagacag caaccccagg ccaaccgtct 12000 atcggccatc atggaagctg tagtgccttc ccgctctaat cccactcatg agaaggtcct 12060 ggccatcgtg aacgcgttgg tggagaacaa agctattcgt ccagatgagg ccggactggt 12120 atacaacgct ctcttagaac gcgtggctcg ctacaacagt agcaatgtgc aaaccaattt 12180 ggaccgtatg ataacagatg tacgcgaagc cgtgtctcag cgcgaaaggt tccagcgtga 12240 tgccaacctg ggttcgctgg tggcgttaaa tgctttcttg agtactcagc ctgctaatgt 12300 gccgcgtggt caacaggatt atactaactt tttaagtgct ttgagactga tggtatcaga 12360 agtacctcag agcgaagtgt atcagtccgg tcctgattac ttctttcaga ctagcagaca 12420 gggcttgcag acggtaaatc tgagccaagc ttttaaaaac cttaaaggtt tgtggggagt 12480 gcatgccccg gtaggagaaa gagcaaccgt gtctagcttg ttaactccga actcccgcct 12540 attattactg ttggtagctc ctttcaccga cagcggtagc atcgaccgta attcctattt 12600 gggttaccta ctaaacctgt atcgcgaagc catagggcaa agtcaggtgg acgagcagac 12660 ctatcaagaa attacccaag tcagtcgcgc tttgggacag gaagacactg gcagtttgga 12720 agccactctg aacttcttgc ttaccaatcg gtctcaaaag atccctcctc aatatgctct 12780 tactgcggag gaggagagga tccttagata tgtgcagcag agcgtgggat tgtttctgat 12840 gcaagagggg gcaactccga ctgcagcact ggacatgaca gcgcgaaata tggagcccag 12900 catgtatgcc agtaaccgac ctttcattaa caaactgctg gactacttgc acagagctgc 12960 cgctatgaac tctgattatt tcaccaatgc catcttaaac ccgcactggc tgcccccacc 13020 tggtttctac acgggcgaat atgacatgcc cgaccctaat gacggatttc tgtgggacga 13080 cgtggacagc gatgtttttt cacctctttc tgatcatcgc acgtggaaaa aggaaggcgg 13140 cgatagaatg cattcttctg catcgctgtc cggggtcatg ggtgctaccg cggctgagcc 13200 cgagtctgca agtccttttc ctagtctacc cttttctcta cacagtgtac gtagcagcga 13260 agtgggtaga ataagtcgcc cgagtttaat gggcgaagag gagtatctaa acgattcctt 13320 gctcagaccg gcaagagaaa aaaatttccc aaacaatgga atagaaagtt tggtggataa 13380 aatgagtaga tggaagactt atgctcagga tcacagagac gagcctggga tcatggggat 13440 tacaagtaga gcgagccgta gacgccagcg ccatgacaga cagaggggtc ttgtgtggga 13500 cgatgaggat tcggccgatg atagcagcgt gctggacttg ggtgggagag gaaggggcaa 13560 cccgtttgct catttgcgcc ctcgcttggg tggtatgttg taaaaaaaaa taaaaaaaaa 13620 actcaccaag gccatggcga cgagcgtacg ttcgttcttc tttattatct gtgtctagta 13680 taatgaggcg agtcgtgcta ggcggagcgg tggtgtatcc ggagggtcct cctccttcgt 13740 acgagagcgt gatgcagcag cagcaggcga cggcggtgat gcaatcccca ctggaggctc 13800 cctttgtgcc tccgcgatac ctggcaccta cggagggcag aaacagcatt cgttattcgg 13860 aactggcacc tcagtacgat accaccaggt tgtatctggt ggacaacaag tcggcggaca 13920 ttgcttctct gaactatcag aatgaccaca gcaacttctt gaccacggtg gtgcaaaaca 13980 atgactttac ccctacggaa gccagcaccc agaccattaa ctttgatgaa cgatcgcggt 14040 ggggcggtca gctaaagacc atcatgcata ctaacatgcc aaacgtgaac gagtatatgt 14100 ttagtaacaa gttcaaagcg cgtgtgatgg tgtccagaaa acctcccgac ggtgctgcag 14160 ttggggatac ttatgatcac aagcaggata ttttgaaata tgagtggttc gagtttactt 14220 tgccagaagg caacttttca gttactatga ctattgattt gatgaacaat gccatcatag 14280 ataattactt gaaagtgggt agacagaatg gagtgcttga aagtgacatt ggtgttaagt 14340 tcgacaccag gaacttcaag ctgggatggg atcccgaaac caagttgatc atgcctggag 14400 tgtatacgta tgaagccttc catcctgaca ttgtcttact gcctggctgc ggagtggatt 14460 ttaccgagag tcgtttgagc aaccttcttg gtatcagaaa aaaacagcca tttcaagagg 14520 gttttaagat tttgtatgaa gatttagaag gtggtaatat tccggccctc ttggatgtag 14580 atgcctatga gaacagtaag aaagaacaaa aagccaaaat agaagctgct acagctgctg 14640 cagaagctaa ggcaaacata gttgccagcg actctacaag ggttgctaac gctggagagg 14700 tcagaggaga caattttgcg ccaacacctg ttccgactgc agaatcatta ttggccgatg 14760 tgtctgaagg aacggacgtg aaactcacta ttcaacctgt agaaaaagat agtaagaata 14820 gaagctataa tgtgttggaa gacaaaatca acacagccta tcgcagttgg tatctttcgt 14880 acaattatgg cgatcccgaa aaaggagtgc gttcctggac attgctcacc acctcagatg 14940 tcacctgcgg agcagagcag gtctactggt cgcttccaga catgatgaag gatcctgtca 15000 ctttccgctc cactagacaa gtcagtaact accctgtggt gggtgcagag cttatgcccg 15060 tcttctcaaa gagcttctac aacgaacaag ctgtgtactc ccagcagctc cgccagtcca 15120 cctcgcttac gcacgtcttc aaccgctttc ctgagaacca gattttaatc cgtccgccgg 15180 cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15240 cgttgcgcag cagtatccgg ggagtccaac gtgtgaccgt tactgacgcc agacgccgca 15300 cctgtcccta cgtgtacaag gcactgggca tagtcgcacc gcgcgtcctt tcaagccgca 15360 ctttctaaaa aaaaaaaaaa tgtccattct tatctcgccc agtaataaca ccggttgggg 15420 tctgcgcgct ccaagcaaga tgtacggagg cgcacgcaaa cgttctaccc aacatcctgt 15480 ccgtgttcgc ggacattttc gcgctccatg gggcgccctc aagggccgca ctcgcgttcg 15540 aaccaccgtc gatgatgtaa tcgatcaggt ggttgccgac gcccgtaatt atactcctac 15600 tgcgcctaca tctactgtgg atgcagttat tgacagtgta gtggctgacg ctcgcaacta 15660 tgctcgacgt aagagccggc gaaggcgcat tgccagacgc caccgagcta ccactgccat 15720 gcgagccgca agagctctgc tacgaagagc tagacgcgtg gggcgaagag ccatgcttag 15780 ggcggccaga cgtgcagctt cgggcgccag cgccggcagg tcccgcaggc aagcagccgc 15840 tgtcgcagcg gcgactattg ccgacatggc ccaatcgcga agaggcaatg tatactgggt 15900 gcgtgacgct gccaccggtc aacgtgtacc cgtgcgcacc cgtccccctc gcacttagaa 15960 gatactgagc agtctccgat gttgtgtccc agcggcgagg atgtccaagc gcaaatacaa 16020 ggaagaaatg ctgcaggtta tcgcacctga agtctacggc caaccgttga aggatgaaaa 16080 aaaaccccgc aaaatcaagc gggttaaaaa ggacaaaaaa gaagaggaag atggcgatga 16140 tgggctggcg gagtttgtgc gcgagtttgc cccacggcga cgcgtgcaat ggcgtgggcg 16200 caaagttcga catgtgttga gacctggaac ttcggtggtc tttacacccg gcgagcgttc 16260 aagcgctact tttaagcgtt cctatgatga ggtgtacggg gatgatgata ttcttgagca 16320 ggcggctgac cgattaggcg agtttgctta tggcaagcgt agtagaataa cttccaagga 16380 tgagacagtg tcgataccct tggatcatgg aaatcccacc cctagtctta aaccggtcac 16440 tttgcagcaa gtgttacccg taactccgcg aacaggtgtt aaacgcgaag gtgaagattt 16500 gtatcccact atgcaactga tggtacccaa acgccagaag ttggaggacg ttttggagaa 16560 agtaaaagtg gatccagata ttcaacctga ggttaaagtg agacccatta agcaggtagc 16620 gcctggtctg ggggtacaaa ctgtagacat taagattccc actgaaagta tggaagtgca 16680 aactgaaccc gcaaagccta ctgccacctc cactgaagtg caaacggatc catggatgcc 16740 catgcctatt acaactgacg ccgccggtcc cactcgaaga tcccgacgaa agtacggtcc 16800 agcaagtctg ttgatgccca attatgttgt acacccatct attattccta ctcctggtta 16860 ccgaggcact cgctactatc gcagccgaaa cagtacctcc cgccgtcgcc gcaagacacc 16920 tgcaaatcgc agtcgtcgcc gtagacgcac aagcaaaccg actcccggcg ccctggtgcg 16980 gcaagtgtac cgcaatggta gtgcggaacc tttgacactg ccgcgtgcgc gttaccatcc 17040 gagtatcatc acttaatcaa tgttgccgct gcctccttgc agatatggcc ctcacttgtc 17100 gccttcgcgt tcccatcact ggttaccgag gaagaaactc gcgccgtaga agagggatgt 17160 tgggacgcgg aatgcgacgc tacaggcgac ggcgtgctat ccgcaagcaa ttgcggggtg 17220 gttttttacc agccttaatt ccaattatcg ctgctgcaat tggcgcgata ccaggcatag 17280 cttccgtggc ggttcaggcc tcgcaacgac attgacattg gaaaaaaacg tataaataaa 17340 aaaaaaaaaa tacaatggac tctgacactc ctggtcctgt gactatgttt tcttagagat 17400 ggaagacatc aatttttcat ccttggctcc gcgacacggc acgaagccgt acatgggcac 17460 ctggagcgac atcggcacga gccaactgaa cgggggcgcc ttcaattgga gcagtatctg 17520 gagcgggctt aaaaattttg gctcaaccat aaaaacatac gggaacaaag cttggaacag 17580 cagtacagga caggcgctta gaaataaact taaagaccag aacttccaac aaaaagtagt 17640 cgatgggata gcttccggca tcaatggagt ggtagatttg gctaaccagg ctgtgcagaa 17700 aaagataaac agtcgtttgg acccgccgcc agcaacccca ggtgaaatgc aagtggagga 17760 agaaattcct ccgccagaaa aacgaggcga caagcgtccg cgtcccgatt tggaagagac 17820 gctggtgacg cgcgtagatg aaccgccttc ttatgaggaa gcaacgaagc ttggaatgcc 17880 caccactaga ccgatagccc caatggccac cggggtgatg aaaccttctc agttgcatcg 17940 acccgtcacc ttggatttgc cccctccccc tgctgctact gctgtacccg cttctaagcc 18000 tgtcgctgcc ccgaaaccag tcgccgtagc caggtcacgt cccgggggcg ctcctcgtcc 18060 aaatgcgcac tggcaaaata ctctgaacag catcgtgggt ctaggcgtgc aaagtgtaaa 18120 acgccgtcgc tgcttttaat taaatatgga gtagcgctta acttgcctat ctgtgtatat 18180 gtgtcattac acgccgtcac agcagcagag gaaaaaagga agaggtcgtg cgtcgacgct 18240 gagttacttt caagatggcc accccatcga tgctgcccca atgggcatac atgcacatcg 18300 ccggacagga tgcttcggag tacctgagtc cgggtctggt gcagttcgcc cgcgccacag 18360 acacctactt caatctggga aataagttta gaaatcccac cgtagcgccg acccacgatg 18420 tgaccaccga ccgtagccag cggctcatgt tgcgcttcgt gcccgttgac cgggaggaca 18480 atacatactc ttacaaagtg cggtacaccc tggccgtggg cgacaacaga gtgctggata 18540 tggccagcac gttctttgac attaggggtg tgttggacag aggtcccagt ttcaaaccct 18600 attctggtac ggcttacaac tccctggctc ctaaaggcgc tccaaataca tctcagtgga 18660 ttgcagaagg tgtaaaaaat acaactggtg aggaacacgt aacagaagag gaaaccaata 18720 ctactactta cacttttggc aatgctcctg taaaagctga agctgaaatt acaaaagaag 18780 gactcccagt aggtttggaa gtttcagatg aagaaagtaa accgatttat gctgataaaa 18840 catatcagcc agaacctcag ctgggagatg aaacttggac tgaccttgat ggaaaaaccg 18900 aaaagtatgg aggcagggct ctcaaacccg atactaagat gaaaccatgc tacgggtcct 18960 ttgccaaacc tactaatgtg aaaggcggtc aggcaaaaca aaaaacaacg gagcagccaa 19020 atcagaaagt cgaatatgat atcgacatgg agttttttga tgcggcatcg cagaaaacaa 19080 acttaagtcc taaaattgtc atgtatgcag aaaatgtaaa tttggaaact ccagacactc 19140 atgtagtgta caaacctgga acagaagaca caagttccga agctaatttg ggacaacaat 19200 ctatgcccaa cagacccaac tacattggct tcagagataa ctttattgga cttatgtact 19260 ataacagtac tggtaacatg ggggtgctgg ctggtcaagc gtctcagtta aatgcagtgg 19320 ttgacttgca ggacagaaac acagaacttt cttaccaact cttgcttgac tctctgggcg 19380 acagaaccag atactttagc atgtggaatc aggctgtgga cagttatgat cctgatgtac 19440 gtgttattga aaatcatggt gtggaagatg aacttcccaa ctactgtttt ccactggacg 19500 gcataggtgt tccaacaacc agttacaaat caatagttcc aaatggagac aatgcgccta 19560 attggaagga acctgaagta aatggaacaa gtgagatcgg acagggtaat ttgtttgcca 19620 tggaaattaa ccttcaagcc aatctatggc gaagtttcct ttattccaat gtggctctat 19680 atctcccaga ctcgtacaaa tacaccccgt ccaatgtcac tcttccagaa aacaaaaaca 19740 cctacgacta catgaacggg cgggtggtgc cgccatctct agtagacacc tatgtgaaca 19800 ttggtgccag gtggtctctg gatgccatgg acaatgtcaa cccattcaac caccaccgta 19860 acgctggctt gcgttaccga tccatgcttc tgggtaacgg acgttatgtg cctttccaca 19920 tacaagtgcc tcaaaaattc ttcgctgtta aaaacctgct gcttctccca ggctcctaca 19980 cttatgagtg gaactttagg aaggatgtga acatggttct acagagttcc ctcggtaacg 20040 acctgcgggt agatggcgcc agcatcagtt tcacgagcat caacctctat gctacttttt 20100 tccccatggc tcacaacacc gcttccaccc ttgaagccat gctgcggaat gacaccaatg 20160 atcagtcatt caacgactac ctatctgcag ctaacatgct ctaccccatt cctgccaatg 20220 caaccaatat tcccatttcc attccttctc gcaactgggc ggctttcaga ggctggtcat 20280 ttaccagact gaaaaccaaa gaaactccct ctttggggtc tggatttgac ccctactttg 20340 tctattctgg ttctattccc tacctggatg gtaccttcta cctgaaccac acttttaaga 20400 aggtttccat catgtttgac tcttcagtga gctggcctgg aaatgacagg ttactatctc 20460 ctaacgaatt tgaaataaag cgcactgtgg atggcgaagg ctacaacgta gcccaatgca 20520 acatgaccaa agactggttc ttggtacaga tgctcgccaa ctacaacatc ggctatcagg 20580 gcttctacat tccagaagga tacaaagatc gcatgtattc atttttcaga aacttccagc 20640 ccatgagcag gcaggtggtt gatgaggtca attacaaaga cttcaaggcc gtcgccatac 20700 cctaccaaca caacaactct ggctttgtgg gttacatggc tccgaccatg cgccaaggtc 20760 aaccctatcc cgctaactat ccctatccac tcattggaac aactgccgta aatagtgtta 20820 cgcagaaaaa gttcttgtgt gacagaacca tgtggcgcat accgttctcg agcaacttca 20880 tgtctatggg ggcccttaca gacttgggac agaatatgct ctatgccaac tcagctcatg 20940 ctctggacat gacctttgag gtggatccca tggatgagcc caccctgctt tatcttctct 21000 tcgaagtttt cgacgtggtc agagtgcatc agccacaccg cggcatcatc gaggcagtct 21060 acctgcgtac accgttctcg gccggtaacg ctaccacgta agaagcttct tgcttcttgc 21120 aaatagcagc tgcaaccatg gcctgcggat cccaaaacgg ctccagcgag caagagctca 21180 gagccattgt ccaagacctg ggttgcggac cctatttttt gggaacctac gataagcgct 21240 tcccggggtt catggccccc gataagctcg cctgtgccat tgtaaatacg gccggacgtg 21300 agacgggggg agagcactgg ttggctttcg gttggaaccc acgttctaac acctgctacc 21360 tttttgatcc ttttggattc tcggatgatc gtctcaaaca gatttaccag tttgaatatg 21420 agggtctcct gcgccgcagc gctcttgcta ccaaggaccg ctgtattacg ctggaaaaat 21480 ctacccagac cgtgcagggt ccccgttctg ccgcctgcgg acttttctgc tgcatgttcc 21540 ttcacgcctt tgtgcactgg cctgaccgtc ccatggacgg aaaccccacc atgaaattgc 21600 taactggagt gccaaacaac atgcttcatt ctcctaaagt ccagcccacc ctgtgtgaca 21660 atcaaaaagc actctaccat tttcttaata cccattcgcc ttattttcgc tcccatcgta 21720 cacacatcga aagggccact gcgttcgacc gtatggatgt tcaataatga ctcatgtaaa 21780 caacgtgttc aataaacatc actttatttt tttacatgta tcaaggctct gcattactta 21840 tttatttaca agtcgaatgg gttctgacga gaatcagaat gacccgcagg cagtgatacg 21900 ttgcggaact gatacttggg ttgccacttg aattcgggaa tcaccaactt gggaaccggt 21960 atatcgggca ggatgtcact ccacagcttt ctggtcagct gcaaagctcc aagcaggtca 22020 ggagccgaaa tcttgaaatc acaattagga ccagtgcttt gagcgcgaga gttgcggtac 22080 accggattgc agcactgaaa caccatcagc gacggatgtc tcacgcttgc cagcacggtg 22140 ggatctgcaa tcatgcccac atccagatct tcagcattgg caatgctgaa cggggtcatc 22200 ttgcaggtct gcctacccat ggcgggcacc caattaggct tgtggttgca atcgcagtgc 22260 agggggatca gtatcatctt ggcctgatcc tgtctgattc ctggatacac ggctctcatg 22320 aaagcatcat attgcttgaa agcctgctgg gctttactac cctcggtata aaacatcccg 22380 caggacctgc tcgaaaactg gttagctgca cagccggcat cattcacaca gcagcgggcg 22440 tcattgttag ctatttgcac cacacttctg ccccagcggt tttgggtgat tttggttcgc 22500 tcgggattct cctttaaggc tcgttgtccg ttctcgctgg ccacatccat ctcgataatc 22560 tgctccttct gaatcataat attgccatgc aggcacttca gcttgccctc ataatcattg 22620 cagccatgag gccacaacgc acagcctgta cattcccaat tatggtgggc gatctgagaa 22680 aaagaatgta tcattccctg cagaaatctt cccatcatcg tgctcagtgt cttgtgacta 22740 gtgaaagtta actggatgcc tcggtgctcc tcgtttacgt actggtgaca gatgcgcttg 22800 tattgttcgt gttgctcagg cattagttta aaagaggttc taagttcgtt atccagcctg 22860 tacttctcca tcagcagaca catcacttcc atgcctttct cccaagcaga caccaggggc 22920 aagctaatcg gattcttaac agtgcaggca gcagctcctt tagccagagg gtcatcttta 22980 gcgatcttct caatgcttct tttgccatcc ttctcaacga tgcgcacggg cgggtagctg 23040 aaacccactg ctacaagttg cgcctcttct ctttcttctt cgctgtcttg actgatgtct 23100 tgcatgggga tatgtttggt cttccttggc ttctttttgg ggggtatcgg aggaggagga 23160 ctgtcgctcc gttccggaga cagggaggat tgtgacgttt cgctcaccat taccaactga 23220 ctgtcggtag aagaacctga ccccacacgg cgacaggtgt ttctcttcgg gggcagaggt 23280 ggaggcgatt gcgaagggct gcggtccgac ctggaaggcg gatgactggc agaacccctt 23340 ccgcgttcgg gggtgtgctc cctgtggcgg tcgcttaact gatttccttc gcggctggcc 23400 attgtgttct cctaggcaga gaaacaacag acatggaaac tcagccattg ctgtcaacat 23460 cgccacgagt gccatcacat ctcgtcctca gcgacgagga aaaggagcag agcttaagca 23520 ttccaccgcc cagtcctgcc accacctcta ccctagaaga taaggaggtc gacgcatctc 23580 atgacatgca gaataaaaaa gcgaaagagt ctgagacaga catcgagcaa gacccgggct 23640 atgtgacacc ggtggaacac gaggaagagt tgaaacgctt tctagagaga gaggatgaaa 23700 actgcccaaa acaacgagca gataactatc accaagatgc tggaaatagg gatcagaaca 23760 ccgactacct catagggctt gacggggaag acgcgctcct taaacatcta gcaagacagt 23820 cgctcatagt caaggatgca ttattggaca gaactgaagt gcccatcagt gtggaagagc 23880 tcagccgcgc ctacgagctt aacctctttt cacctcgtac tccccccaaa cgtcagccaa 23940 acggcacctg cgagccaaat cctcgcttaa acttttatcc agcttttgct gtgccagaag 24000 tactggctac ctatcacatc ttttttaaaa atcaaaaaat tccagtctcc tgccgcgcta 24060 atcgcacccg cgccgatgcc ctactcaatc tgggacctgg ttcacgctta cctgatatag 24120 cttccttgga agaggttcca aagatcttcg agggtctggg caataatgag actcgggccg 24180 caaatgctct gcaaaaggga gaaaatggca tggatgagca tcacagcgtt ctggtggaat 24240 tggaaggcga taatgccaga ctcgcagtac tcaagcgaag catcgaggtc acacacttcg 24300 catatcccgc tgtcaacctg ccccctaaag tcatgacggc ggtcatggac cagttactca 24360 ttaagcgcgc aagtcccctt tcagaagaca tgcatgaccc agatgcctgt gatgagggta 24420 aaccagtggt cagtgatgag cagctaaccc gatggctggg caccgactct cccagggatt 24480 tggaagagcg tcgcaagctt atgatggccg tggtgctggt taccgtagaa ctagagtgtc 24540 tccgacgttt ctttaccgat tcagaaacct tgcgcaaact cgaagagaat ctgcactaca 24600 cttttagaca cggctttgtg cggcaggcat gcaagatatc taacgtggaa ctcaccaacc 24660 tggtttccta catgggtatt ctgcatgaga atcgcctagg acaaagcgtg ctgcacagca 24720 ccctgaaggg ggaagcccgc cgtgattaca tccgcgattg tgtctatctg tacctgtgcc 24780 acacgtggca aaccggcatg ggtgtatggc agcaatgttt agaagaacag aacttgaaag 24840 agcttgacaa gctcttacag aaatctctta aggttctgtg gacagggttc gacgagcgca 24900 ccgtcgcttc cgacctggca gacctcatct tcccagagcg tctcagggtt actttgcgaa 24960 acggattgcc tgactttatg agccagagca tgcttaacaa ttttcgctct ttcatcctgg 25020 aacgctccgg tatcctgccc gccacctgct gcgcactgcc ctccgacttt gtgcctctca 25080 cctaccgcga gtgccccccg ccgctatgga gtcactgcta cctgttccgt ctggccaact 25140 atctctccta ccactcggat gtgatcgagg atgtgagcgg agacggcttg ctggagtgtc 25200 actgccgctg caatctgtgc acgccccacc ggtccctagc ttgcaacccc cagttgatga 25260 gcgaaaccca gataataggc acctttgaat tgcaaggccc cagcagccaa ggcgatgggt 25320 cttctcctgg gcaaagttta aaactgaccc cgggactgtg gacctccgcc tacttgcgca 25380 agtttgctcc ggaagattac cacccctatg aaatcaagtt ctatgaggac caatcacagc 25440 ctccaaaggc cgaactttcg gcctgcgtca tcacccaggg ggcaattctg gcccaattgc 25500 aagccatcca aaaatcccgc caagaatttc tactgaaaaa gggtaagggg gtctaccttg 25560 acccccagac cggcgaggaa ctcaacacaa ggttccctca ggatgtccca acgacgagaa 25620 aacaagaagt tgaaggtgca gccgccgccc ccagaagata tggaggaaga ttgggacagt 25680 caggcagagg aggcggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gtcaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520 cgccttacat gtggagttat caaccccaaa tgggattggc ggcaggcgcc tcccaggact 26580 actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640 tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700 aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760 ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820 agttagctgg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880 tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940 gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000 ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060 aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120 attacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180 attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240 ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300 ccctcaaggt ccggcccacg gagtgcggat ttctatcgaa ggcaaaatag actctcgcct 27360 gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420 ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480 tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540 gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600 ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660 ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720 tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780 acctatacac accttgcttc actttcttag tggtgttgtg gtattggttt aaaaaatggg 27840 gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900 tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960 catctgtgga gttcatcgcc tctcttacga acttggcccc caacgacaaa aatttacctg 28020 catggtggga atcaacccca tagttatcac ccagcaaagt ggagatacta agggttgcat 28080 tcactgctcc tgcgattcca tcgagtgcac ctacaccctg ctgaagaccc tatgcggcct 28140 aagagacctg ctaccaatga attaaaaaat gattaataaa aaatcactta ctgcggccgc 28200 tgctaatcct ttctctcttc aggccaccat gggatggagc tgtatcatcc tcttcttggt 28260 agcaacagct acaggtgtcc actccgaggt tcagctggtg gagtctggcg gtggcctggt 28320 gcagccaggg ggctcactcc gtttgtcctg tgcagcttct ggctatacct tcaccaacta 28380 tggtatgaac tgggtccgtc aggccccggg taagggcctg gaatgggttg gatggattaa 28440 cacctatacc ggtgaaccga cctatgctgc ggatttcaaa cgtcgtttta ctttttctct 28500 ggacacctcc aagagcacag cttacctgca gatgaacagc ctgcgcgctg aggacactgc 28560 cgtctattac tgtgcaaagt acccgcacta ttatgggagc agccactggt atttcgacgt 28620 ctggggtcaa ggaaccctgg tcaccgtctc ctcgggtggc ggtggctcgg gcggtggtgg 28680 atctggtggc ggcggatctg atatccagat gacccagtcc ccgagctccc tgtccgcctc 28740 tgtgggcgat agggtcacca tcacctgcag cgcaagtcag gatattagca actatttaaa 28800 ctggtatcaa cagaaaccag gaaaagctcc gaaagtcctg atttacttca cctcctctct 28860 ccactctgga gtcccttctc gcttctctgg atccggttct gggacggatt tcactctgac 28920 catcagcagt ctgcagccag aagacttcgc aacttattac tgtcaacagt atagcaccgt 28980 gccgtggacg tttggacagg gtaccaaggt ggagatcaaa cgacatcatc accaccacca 29040 ttaagctagc ttgactgact gagatacagc gtaccttcag ctcacagaca tgataagata 29100 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 29160 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 29220 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 29280 caagtaaaac ctctacaaat gtgggccggc ctgaaatcag caataaggtc tctgttgaaa 29340 ttttctccca gcagcacctc acttccctct tcccaactct ggtattctaa accccgttca 29400 gcggcatact ttctccatac tttaaagggg atgtcaaatt ttagctcctc tcctgtaccc 29460 acaatcttca tgtctttctt cccagatgac caagagagtc cggctcagtg actccttcaa 29520 ccctgtctac ccctatgaag atgaaagcac ctcccaacac ccctttataa acccagggtt 29580 tatttcccca aatggcttca cacaaagccc aaacggagtt cttactttaa aatgtttaac 29640 cccactaaca accacaggcg gatctctaca gctaaaagtg ggagggggac ttacagtgga 29700 tgacaccaac ggttttttga aagaaaacat aagtgccacc acaccactcg ttaagactgg 29760 tcactctata ggtttaccac taggagccgg attgggaacg aatgaaaata aactttgtat 29820 caaattagga caaggactta cattcaattc aaacaacatt tgcattgatg acaatattaa 29880 caccttatgg acaggagtca accccaccga agccaactgt caaatcatga actccagtga 29940 atctaatgat tgcaaattaa ttctaacact agttaaaact ggagcactag tcactgcatt 30000 tgtttatgtt ataggagtat ctaacaattt taatatgcta actacacaca gaaatataaa 30060 ttttactgca gagctgtttt tcgattctac tggtaattta ctaactagac tctcatccct 30120 caaaactcca cttaatcata aatcaggaca aaacatggct actggtgcca ttactaatgc 30180 taaaggtttc atgcccagca cgactgccta tcctttcaat gataattcta gagaaaaaga 30240 aaactacatt tacggaactt gttactacac agctagtgat cgcactgctt ttcccattga 30300 catatctgtc atgcttaacc gaagagcaat aaatgacgag acatcatatt gtattcgtat 30360 aacttggtcc tggaacacag gagatgcccc agaggtgcaa acctctgcta caaccctagt 30420 cacctcccca tttacctttt actacatcag agaagacgac tgacaaataa agtttgcgat 30480 cgctaccctg caggaacttg tttatttgaa aatcaattca caaaatccga gtagttattt 30540 tgcctccccc ttcccattta acagaataca ccaatctctc cccacgcaca gctttaaaca 30600 tttggatacc attagatata gacatggttt tagattccac attccaaaca gtttcagagc 30660 gagccaatct ggggtcagtg atagataaaa atccatcggg atagtctttt aaagcgcttt 30720 cacagtccaa ctgctgcgga tgcgactccg gagtctggat cacggtcatc tggaagaaga 30780 acgatgggaa tcataatccg aaaacggtat cggacgattg tgtctcatca aacccacaag 30840 cagccgctgt ctgcgtcgct ccgtgcgact gctgtttatg ggatcagggt ccacagtgtc 30900 ctgaagcatg attttaatag cccttaacat caactttctg gtgcgatgcg cgcagcaacg 30960 cattctgatt tcactcaaat ctttgcagta ggtacaacac attattacaa tattgtttaa 31020 taaaccataa ttaaaagcgc tccagccaaa actcatatct gatataatcg cccctgcatg 31080 accatcatac caaagtttaa tataaattaa atgacgttcc ctcaaaaaca cactacccac 31140 atacatgatc tcttttggca tgtgcatatt aacaatctgt ctgtaccatg gacaacgttg 31200 gttaatcatg caacccaata taaccttccg gaaccacact gccaacaccg ctcccccagc 31260 catgcattga agtgaaccct gctgattaca atgacaatga agaacccaat tctctcgacc 31320 gtgaatcact tgagaatgaa aaatatctat agtggcacaa catagacata aatgcatgca 31380 tcttctcata atttttaact cctcaggatt tagaaacata tcccagggaa taggaagctc 31440 ttgcagaaca gtaaagctgg cagaacaagg aagaccacga acacaactta cactatgcat 31500 agtcatagta tcacaatctg gcaacagcgg gtggtcttca gtcatagaag ctcgggtttc 31560 attttcctca caacgtggta actgggctct ggtgtaaggg tgatgtctgg cgcatgatgt 31620 cgagcgtgcg cgcaaccttg tcataatgga gttgcttcct gacattctcg tattttgtat 31680 agcaaaacgc ggccctggca gaacacactc ttcttcgcct tctatcctgc cgcttagcgt 31740 gttccgtgtg atagttcaag tacaaccaca ctcttaagtt ggtcaaaaga atgctggctt 31800 cagttgtaat caaaactcca tcgcatctaa tcgttctgag gaaatcatcc aagcaatgca 31860 actggattgt gtttcaagca ggagaggaga gggaagagac ggaagaacca tgttaatttt 31920 tattccaaac gatctcgcag tacttcaaat tgtagatcgc gcagatggca tctctcgccc 31980 ccactgtgtt ggtgaaaaag cacagctaga tcaaaagaaa tgcgattttc aaggtgctca 32040 acggtggctt ccagcaaagc ctccacgcgc acatccaaga acaaaagaat accaaaagaa 32100 ggagcatttt ctaactcctc aatcatcata ttacattcct gcaccattcc cagataattt 32160 tcagctttcc agccttgaat tattcgtgtc agttcttgtg gtaaatccaa tccacacatt 32220 acaaacaggt cccggagggc gccctccacc accattctta aacacaccct cataatgaca 32280 aaatatcttg ctcctgtgtc acctgtagcg aattgagaat ggcaacatca attgacatgc 32340 ccttggctct aagttcttct ttaagttcta gttgtaaaaa ctctctcata ttatcaccaa 32400 actgcttagc cagaagcccc ccgggaacaa gagcagggga cgctacagtg cagtacaagc 32460 gcagacctcc ccaattggct ccagcaaaaa caagattgga ataagcatat tgggaaccgc 32520 cagtaatatc atcgaagttg ctggaaatat aatcaggcag agtttcttgt aaaaattgaa 32580 taaaagaaaa atttgccaaa aaaacattca aaacctctgg gatgcaaatg caataggtta 32640 ccgcgctgcg ctccaacatt gttagttttg aattagtctg caaaaataaa aaaaaaaaca 32700 agcgtcatat catagtagcc tgacgaacag atggataaat cagtctttcc atcacaagac 32760 aagccacagg gtctccagct cgaccctcgt aaaacctgtc atcatgatta aacaacagca 32820 ccgaaagttc ctcgcggtga ccagcatgaa taattcttga tgaagcatac aatccagaca 32880 tgttagcatc agttaacgag aaaaaacagc caacatagcc tttgggtata attatgctta 32940 atcgtaagta tagcaaagcc acccctcgcg gatacaaagt aaaaggcaca ggagaataaa 33000 aaatataatt atttctctgc tgctgttcag gcaacgtcgc ccccggtccc tctaaataca 33060 catacaaagc ctcatcagcc atggcttacc agacaaagta cagcgggcac acaaagcaca 33120 agctctaaag tgactctcca acctctccac aatatatata tacacaagcc ctaaactgac 33180 gtaatgggag taaagtgtaa aaaatcccgc caaacccaac acacaccccg aaactgcgtc 33240 accagggaaa agtacagttt cacttccgca atcccaacag gcgtaacttc ctctttctca 33300 cggtacgtga tatcccacta acttgcaacg tcattttccc acggtcgcac cgcccctttt 33360 agccgttaac cccacagcca atcaccacac gatccacact ttttaaaatc acctcattta 33420 catattggca ccattccatc tataaggtat attatataga taga 33464

Claims (22)

  1. 아데노바이러스의 제조를 위한 연속 공정으로서,
    A) 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 상기 아데노바이러스에 의해 감염된 포유류 세포를 연속하여 배양하는 단계로서, 상기 세포는 바이러스 복제를 지지할 수 있는, 상기 배양하는 단계, 및
    B) 상기 배지로부터 단계 a)로부터 제조된 바이러스를 단리하는 단계로서, 바이러스의 단리는 세포 용해 단계에 후속하지 않는, 상기 단리하는 단계를 포함하되,
    바이러스 감염 및 제조를 위한 생존가능 세포는 연속 제조의 기간 동안 상기 바이러스를 복제하기에 적합한 수준으로 상기 용기 내에 유지되고,
    상기 공정은 적어도 하나의 배지 변경 또는 첨가를 포함하고, 감염 후 적어도 일 시점에서 상기 세포의 적어도 일부는 변경되거나 세포가 첨가되는, 아데노바이러스의 제조를 위한 연속 공정.
  2. 제1항에 있어서, 상기 바이러스는 B 그룹 아데노바이러스, 예를 들어, Ad11로부터의 헥손 및 섬유를 가지고, 특히 바이러스는 EnAd인, 아데노바이러스의 제조를 위한 연속 공정.
  3. 제1항 또는 제2항에 있어서, 상기 바이러스는 복제 가능(replication competent)한, 아데노바이러스의 제조를 위한 연속 공정.
  4. 제1항 내지 제3항 중 어느 한 항에 있어서, 상기 연속 제조 기간은 적어도 2회의 바이러스 복제 주기를 포함하는, 아데노바이러스의 제조를 위한 연속 공정.
  5. 제1항 내지 제4항 중 어느 한 항에 있어서, 각각의 바이러스 복제 주기는 30시간 내지 300시간, 예컨대 70시간 내지 300시간, 또는 30시간 내지 100시간, 예컨대 60시간, 70시간, 80시간, 90시간, 95시간 또는 100시간의 범위인, 아데노바이러스의 제조를 위한 연속 공정.
  6. 제1항 내지 제5항 중 어느 한 항에 있어서, 상기 공정은 감염 후 일 이상의 시점에서 세포마다 적어도 50,000개의 바이러스 입자를 제조하는, 아데노바이러스의 제조를 위한 연속 공정.
  7. 제1항 내지 제6항 중 어느 한 항에 있어서, 바이러스 감염 및 제조를 위한 생존가능 세포는 상기 배양물에 대한 첨가 세포에 의해 바이러스를 복제하기에 적합한 수준으로 상기 용기 내에서 유지되는, 아데노바이러스의 제조를 위한 연속 공정.
  8. 제1항 내지 제7항 중 어느 한 항에 있어서, 세포는 감염 후 일 이상의 시점에서 상기 배양물로부터 제거되는, 아데노바이러스의 제조를 위한 연속 공정.
  9. 제1항 내지 제8항 중 어느 한 항에 있어서, 상기 포유류 세포는 HEK, CHO, HeLa, Vero, A549, PerC6 및 GMK, 특히 HEK293을 포함하는 군으로부터 선택되는, 아데노바이러스의 제조를 위한 연속 공정.
  10. 제1항 내지 제9항 중 어느 한 항에 있어서, 감염 다중도(multiplicity of infection)는 5 내지 50vp/세포, 예를 들어 5 내지 49, 예컨대 10 내지 20, 특히 12.5vp/세포인, 아데노바이러스의 제조를 위한 연속 공정.
  11. 제1항 내지 제10항 중 어느 한 항에 있어서, 상기 세포는 1 내지 4×106vp/㎖의 바이러스의 시작 농도에 의해 감염된, 아데노바이러스의 제조를 위한 연속 공정.
  12. 제1항 내지 제11항 중 어느 한 항에 있어서, 관류 배양이 이용되는, 아데노바이러스의 제조를 위한 연속 공정.
  13. 제1항 내지 제12항 중 어느 한 항에 있어서, 현탁 배양이 이용되는, 아데노바이러스의 제조를 위한 연속 공정.
  14. 제1항 내지 제12항 중 어느 한 항에 있어서, 부착 배양이 이용되는, 아데노바이러스의 제조를 위한 연속 공정.
  15. 제1항 내지 제14항 중 어느 한 항에 있어서, 상기 공정은 CsCl 구배, 크로마토그래피 단계, 예컨대 이온 교환 크로마토그래피, 특히 음이온 교환 크로마토그래피, 및 이들의 조합으로부터 선택된 정제 단계를 추가로 포함하는, 아데노바이러스의 제조를 위한 연속 공정.
  16. 제1항 내지 제15항 중 어느 한 항에 있어서, 적어도 하나의 배지 변경 또는 첨가를 포함하는, 아데노바이러스의 제조를 위한 연속 공정.
  17. 제1항 내지 제18항 중 어느 한 항에 있어서, 저장에 적합한 완충제 중에 바이러스를 제제화하는 단계를 추가로 포함하는, 아데노바이러스의 제조를 위한 연속 공정.
  18. 아데노바이러스의 제조 공정으로서,
    a. 용기 내에서 바이러스가 복제하도록 세포를 지지하기에 적합한 배지의 존재 하에 상기 아데노바이러스에 의해 감염된 포유류 세포를 배양하는 단계로서, 상기 세포는 바이러스 복제를 지지할 수 있고, 상기 바이러스의 시작 시드 밀도는 1 내지 2×106vp/㎖(예컨대, 1×106vp/㎖)의 범위이고, 감염 다중도는 5 내지 20, 예컨대 10 내지 15, 특히 12.5vp/세포의 범위인, 상기 배양하는 단계, 및
    b. 상기 세포로부터 상기 바이러스를 수확하기 위해 바이러스 감염 후 24시간 내지 75시간의 기간에 용해 단계를 수행하는 단계로서, 예를 들어 상기 용해 단계는 감염 후 65시간 내지 70시간에, 예컨대 감염 후 66시간, 67시간, 68시간 또는 69시간에 수행되는, 상기 수행하는 단계를 포함하는, 아데노바이러스의 제조 공정.
  19. 제18항에 있어서, 상기 바이러스는 B 그룹 바이러스, 예를 들어 EnAd인, 아데노바이러스의 제조 공정.
  20. 제18항 또는 제19항에 있어서, 상기 공정은 적어도 하나의 배지 변경 또는 첨가를 포함하는, 아데노바이러스의 제조 공정.
  21. 제18항 내지 제20항 중 어느 한 항에 있어서, 감염 후 적어도 일 시점에서 상기 세포의 적어도 일부는 변경되거나 세포가 첨가되는, 아데노바이러스의 제조 공정.
  22. 제1항 내지 제19항 중 어느 한 항에 기재된 공정으로부터 얻어지거나 얻어질 수 있는 바이러스 또는 제제.
KR1020177008230A 2014-08-27 2015-08-27 아데노바이러스의 제조를 위한 공정 KR20170044194A (ko)

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