KR20120093604A - Composition and soil conditioner for preventing disease animals, fish and plants - Google Patents

Abstract

PURPOSE: A composition for preventing, controlling, and treating fish diseases, a producing method thereof, and a soil conditioner using the composition are provided to use natural ingredients to obtain the composition. CONSTITUTION: A producing method of a composition for preventing, controlling, and treating fish diseases comprises the following steps: mixing 1-2g of leaf mold and 45-55ml of water with 10ml of saline solution, and removing suspended materials from the upper side of the mixture; obtaining suspension from the mixture, diluting the suspension with the saline solution in a ratio of 1:10, and repeatedly diluting the suspension with the saline solution for 2-4 more times; mixing 1-2g of rice bran powder, 0.1-0.2g of salicornia herbacea powder, and 250-350ml of distilled water for 1ml of suspension; culturing the mixture at room temperature using a bubble generator for separating single strains; cultivating each single strain in an agar medium and a blood medium at 25-35 deg C; subculturing the single strains 3-4 times for separating spawns; mixing 500-700cc of obtained spawns, 250-350kg of salicornia herbacea, and 100-200kg of shiitake powder with 1t of edible underground water; firstly culturing the mixture for 2-4 months using the bubble generator; secondly culturing the mixture after adding artemisia capillaries, spring onion, ginger, and garlic for 1-2 months; and aging the cultured liquid for 1-2 months.

Description

Composition and soil conditioner for preventing, controlling and treating animal and fish diseases using seaweed, rice bran, manjin, shiitake, leek, garlic and ginger, and composition and soil conditioner for preventing disease animals, fish and plants}

The present invention relates to a composition for the prevention and control of diseases and fauna and fish diseases using seaweed, rice bran, jinjin, shiitake, green onion, garlic, ginger, and animal feed additives and soil improver using the same, more specifically 1 ~ 1 2 g and 45-55 ml of water are suspended in 10 ml of physiological saline, suspended to remove suspended solids in the upper layer, the supernatant is taken from the suspended mixture, diluted 1:10 with physiological saline, and then again 2 ~ Repeated dilution four times, 1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of seaweed powder, 250 ~ 350ml of distilled water based on 1ml of the final dilution solution through the dilution step, and incubated at room temperature with a bubble generator, each single strain The isolates were separated and cultured for one week at 25-35 ℃ using nutrient agar and blood medium, and then subcultured three to four times when a single strain appeared. Incubate and mix 500 ~ 700cc of the microorganism spawn, 250 ~ 350kg of seaweed, and 100 ~ 200kg of shiitake mushroom powder, based on 1t of edible ground water, and operate the bubbler to supply oxygen for the first time at room temperature for 2 ~ 4 months. Incubate in 250 ~ 350kg, green onion 150 ~ 250kg, 50 ~ 150kg ginger, 25 ~ 75kg garlic in a mature culture liquid, and then incubated for 2 months at room temperature for 1-2 months, the cultured liquid for about 1-2 months The present invention relates to a method for preparing and controlling a composition and a soil improving agent for preventing, controlling and treating diseases of plants and animals using seaweed, rice bran, jinjin, shiitake, leek, garlic, ginger, and aging. Animal feed additives and pesticide free soil improver compositions.

With the development of modern industry, the functionality of food is changing according to the needs of the times. Recently, as incomes of consumers increase, and as a result, increased leisure activity and increased interest in health, products containing functional substances with physiologically active effects are emerging, and production and products using functional feed products in fish as well as animals and plants The necessity of consumption is highlighted, and the eco-friendliness of plants such as various vegetables on the table of consumers is being emphasized through soil improvement.

The feed industry, meanwhile, has evolved dramatically beyond the past mill levels. In other words, the nutritional formulation of feed for improving the productivity of livestock has developed to the level of developed countries, and the direction of animal drugs and feed additives is changing accordingly.

Generally, "feed additive" refers to a pharmaceutical product (vitamin, provitamin, antibiotic, antibacterial, antioxidant, etc.) added to feed for the purpose of preventing disease, improving nutritional deficiency symptoms, improving feed efficiency and promoting growth. Small amounts added or supplemented to livestock feed based on energy and protein supply, in general sense, to maximize animal life support and animal production, including antifungal agents, enzymes, probiotics, amino acids, and trace minerals. Generically used to increase the effectiveness of the feed.

Until now, additives used in feed have been used for antibiotics and synthetic antibacterial agents. On the other hand, instead of adding drugs to produce hygienic and safe livestock products, additives are used to increase livestock resistance.

In addition, additives for supplementing the nutritional components of the feed may include trace minerals such as vitamins, iron, copper, manganese and iodine, amino acids such as lysine, methionine, and tryptophan.

As described above, the use of additives for livestock itself is already common, and recently, researches on additives have been conducted in order to study the implementation of livestock by administering various additives for improving human health to livestock. In other words, various efforts are being made for the production of healthy livestock products, namely functional livestock products, which are considered so that humans can consume special ingredients in addition to protein sources when ingesting livestock products.

Feed additives are expected to evolve further from simple additions to improve feed quality in the past and to respond appropriately to increased productivity of livestock products and to increased user concerns about food safety and hygiene.

However, feed additives and soil improvers, etc. are added to the chemical components have a direct impact on the flora and fauna and soil, and eventually had a harmful problem to the human body.

The present invention has been invented to solve the above problems, suspended suspension to remove the suspended matter in the upper layer after suspending 1 ~ 2g and 1 ~ 2g water and 45 ~ 55ml of physiological saline. Take the supernatant from and dilute to 1:10 with physiological saline and repeat dilution 2 ~ 4 times, and based on 1ml of the final dilution solution, the rice bran powder 1 ~ 2g, seaweed powder 0.1 ~ 0.2g, distilled water 250 Including ~ 350ml and incubated at room temperature by using a bubble generator to separate each single strain and culture the isolated strains for one week at 25 ~ 35 ℃ each using nutrient agar and blood medium 3 ~ After spawning 4 times subculture, the spawn is separated and mixed with 500 ~ 700cc of microorganism spawn, 250 ~ 350kg of seaweed and 100 ~ 200kg of shiitake mushroom powder based on 1t of edible ground water. Put it in the air and operate the bubbler to supply oxygen and incubate it first at room temperature for 2 ~ 4 months, and add 250 ~ 350kg of Injin mugwort, 150 ~ 250kg of green onion, 50 ~ 150kg of ginger, and 25 ~ 75kg of garlic in the matured liquid. Second culture at room temperature for 2 months, the cultured liquid is formed by aging for 1 to 2 months, and then formulated into powder pellet granules or liquid using natural materials using microorganisms, not chemicals. It can be used as a non-antibiotic microbial agent for the prevention and control of animal and fish diseases without harming animals, fish and fish, and diseases caused by bacteria, fungi, viruses, or diseases caused by infecting animals and fish. On prevention, control and treatment of animal and fish diseases using sea urchin, rice bran, manjin, shiitake, leek, garlic and ginger, which can be used for prevention, growth inhibition and death. Dangerous production process and there is provided by the composition and the soil conditioner them.

Method for producing a composition for the prevention, control, and treatment of diseases and fauna and fish diseases using seaweed, rice bran, phosphorus, shiitake, leek, garlic, ginger, and compositions and soil improvers of the present invention using natural materials using microorganisms, not chemicals When formulated into powder pellets or liquid granules, it can be used as an antibiotic-free microbial agent for preventing and controlling diseases of animals and fish without harming animals and fish without any harm. Parasitic bacteria, fungi, viruses or diseases caused by the disease can be used for prevention and growth prevention, prevention of death, etc. It is very useful effect.

The method of preparing a composition for the prevention, control, and treatment of diseases and fauna and fish diseases using seaweed, rice bran, manjin, shiitake, leeks, garlic, and ginger of the present invention is suspended in 10 ml of physiological saline (1 ~ 2g and 45 ~ 55ml of water). Iv) a suspending step of removing suspended solids in the upper layer; Taking a supernatant from the suspended mixture, diluting with 1:10 with physiological saline, and diluting again 2-4 times; 1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of distilled water, 250 ~ 350ml of distilled water based on the final dilution 1ml of the dilution step and incubated at room temperature with a bubble generator to separate each single strain, separated Spawn culture step of culturing strains at 25-35 ° C. for one week using nutrient agar medium and blood medium to separate three to four times when subcultured (subculture, 繼 代 培養) when a single strain appears; Based on the 1t of edible ground water, 500 ~ 700cc of the microorganism spawn extracted above, 250 ~ 350kg of seaweed and 100 ~ 200kg of shiitake mushrooms are mixed and operated with a bubbler to supply oxygen to incubate at room temperature for 2 ~ 4 months. step; 250-350kg jinjin wormwood, 150-250kg green onions, 50-150kg ginger, 25-75kg garlic in a mature culture liquid, the second culture step of continuing the culture at room temperature for 1-2 months; Characterized in that formed through the aging step of aging the cultured liquid phase 1-2 months.

In addition, the composition of the plant and fish disease prevention and control, treatment, treatment of plants and plants using seaweed, rice bran, manjin, shiitake, leek, garlic, ginger of the present invention is suspended in 10 ml of saline solution 1 ~ 2g and 45 ~ 55ml of water (懸)濁) and then suspended to remove the suspended solids in the upper portion, the supernatant is taken from the suspended mixture, diluted 1:10 with physiological saline, and diluted again 2-4 times, and the final dilution 1ml after the dilution step Rice bran powder 1 ~ 2g, seaweed powder 0.1 ~ 0.2g, distilled water 250 ~ 350ml are added and cultured at room temperature using a bubble generator to separate single strains and nutrient agar medium and blood medium When cultured for 1 week at 25 ~ 35 ℃ each using a single strain appear subculture 3 ~ 4 times subculture (separation) to separate the spawn, and based on 1t of edible ground water microbial spawn Mix 500 ~ 700cc with seaweed 250 ~ 350kg, shiitake mushroom powder 100 ~ 200kg, operate bubbler to supply oxygen and incubate first at room temperature for 2 ~ 4 months. ~ 250kg, ginger 50-150kg, garlic 25-75kg put in a second culture at room temperature for 1-2 months, characterized in that formed by aging the cultured liquid for 1-2 months.

The suspension step is suspended in 1 ~ 2g and 1 ~ 2g water and 45 ~ 55ml of physiological saline (懸 濁) to remove the suspended matter in the upper portion.

This is to remove impurities mixed in the soil and collect effective microorganisms. The reason for mixing the 1 ~ 2g and the water 45 ~ 55ml of the foliar soil is mixed to remove the suspended matter, when the mixture is mixed to 45ml or less, it is difficult to remove the impurities due to the suspension of the crushed soil, if mixed more than 55ml This is because there is a difference in quantity in separating strains at a later stage. Suspension step as described above to remove impurities by suspending 1 ~ 2g and 1 ~ 2g of physiological saline and 45 ~ 55ml of water. Physiological saline refers to the isotonic solution prepared by adjusting the concentration of the body fluid in our body as 0.9% NaCl solution. Unlike normal water, physiological saline does not cause osmotic pressure change even if it enters the blood vessel directly, so that symptoms such as shock do not appear even when injected through Ringer, thereby preventing loss due to osmotic pressure change of effective microorganisms.

In addition, foliar soil is soil formed by decay and degradation of leaves and twigs by microorganisms. It is widely used in horticulture and has good drainage and has a lot of moisture and nutrients. It is most commonly used in horticulture and is very porous, so it has good drainage, strong moisture and nutrient retention, high geothermal properties and rich nutrients. There are artificially collected and deciduous leaves and spoiled naturally. It is also made by digging and burying the ground. Usually, after one year, a quality defoliation soil is produced. Generally, the deciduous leaves of hardwoods are more active than conifers, and their maturity is faster. When using, it is good to mix fertilizer and soil properly.

In the dilution step, the supernatant is taken from the suspension mixture, diluted 1:10 with physiological saline, and diluted 2-4 times again.

The reason for dilution with physiological saline is the same as the reason for the suspension step. The reason for repeating 2-4 times is that the supernatant in the suspended mixture is present in the microorganisms with the least impurities, but the same process is divided into two to four times.

The spawn culture step comprises 1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of seaweed powder, 250 ~ 350ml of distilled water based on 1ml of the final dilution solution after the dilution step, and incubated at room temperature using a bubble generator to incubate each single strain. Isolate and isolate the isolated strain by subculture (3 代 培養) three to four times when a single strain appears by culturing for one week at 25 ~ 35 ℃ each using a nutrient agar medium and blood medium.

The reason for adding 0.1 ~ 0.2g of rice bran powder is that the effect of rice bran does not appear when 0.1g or less is added, and when 0.2g or more is added, the strain is cultured, but because of the interference of rice bran, 0.1-0.2 g of powder is preferred.

In addition, the rice bran is called `` Migang '', which is a name for the skin powder consisting of rice bran and rice snow that are produced during the process of refining rice from brown rice to white rice. When analyzing the nutritional content of rice, 29% of rice bran, 66% of rice snow, and 5% of rice called white rice are distributed, and 95% of the nutrients of rice are contained in rice bran and rice snow, or rice bran. It is. Its effect is that rice bran contains a large amount of pesticides and heavy metals, 'Fuchin acid', which has a detoxifying effect of heavy metals and residual pesticides.In general, the rich ingredients of rice bran improve blood circulation and human's natural healing power. It is known to keep the blood in proper alkaline, and to discharge waste materials and harmful metals in the body.

Hamcho powder is prepared by naturally drying the seaweed for 7 to 15 days and then grinding to make powder. The purpose of natural drying of the seaweed is to prevent the destruction of various minerals and enzymes concentrated in the seaweed. In addition, when dried for less than 7 days, the seaweed is not dried properly, it is difficult to grind.

The reason for adding 0.1 ~ 0.2g of seaweed powder is that the effect of seaweed does not show up when 0.1g or less is added, and when 0.2g or more is added, salt is basically contained in seaweed, which may affect effective microorganisms. Since it is possible, 0.1-0.2 g of seaweed powder is preferable.

In addition, the seaweed grows in groups around rocks or tidal flats that reach the sea waters of islands such as the west coast of Korea or "South coast, Jeju Island, Ulleungdo, and Baekdo". ).

The seaweed is a plant that grows in groups around the sapphire or salt field close to the seawater. It is grown on land and contains various minerals and enzymes contained in the seawater. In seawater, dozens of trace elements and various enzymes such as calcium, potassium, magnesium, iron, iodine and phosphorus are dissolved, and the seaweed grows by absorbing trace elements and enzymes that are beneficial to the human body. Enzymes in seawater decompose and purify various organic matters in seawater. In the seaweed, enzymes of seawater are concentrated in a large amount, and this enzyme removes various toxins accumulated in the human body and breaks down the stool and releases it out of the body.

The seaweed is concentrated in various minerals and enzymes and the specific effects are as follows.

Get rid of stool, fix constipation and treat obesity. The human barrier is densely packed with small bumps called fleece. Food waste in the fleece will not only absorb nutrients from the large intestine, but food will also stick to the barrier. This is called stool, and the seaweed has a function of dissolving it and sending it out of the body. Seaweed breaks down stool to reduce weight and treat constipation. Treat high and low blood pressure Hamcho improves blood circulation, cleans blood, and strengthens blood vessels to treat high and low blood pressure simultaneously. Hamcho strengthens the heart and removes cholesterol and triglycerides in the blood, so that both hypertension and hypotension are healed at the same time. Hamcho is also effective in treating anemia because of its excellent effect. It cures all kinds of inflammations such as sinusitis, nephritis and arthritis. Seaweed is very effective in killing pathogenic microorganisms. There are cases where malignant pleurisy patients who are not effective with any type of antibiotics have taken seaweed and have recovered, and those who have given up their lives with Behcet's disease have recovered. Beautify your skin. Hamcho is an effective cosmetic product that can be said to be a cosmetic to eat. If there is no stool, your skin will be clean. Freckles, freckles, acne and women's menstrual disorders are usually better. Good gastrointestinal function. The seaweed is active in the stomach and colon, which helps to digest and improve constipation, hernia, and hemorrhoids. Eating seaweed makes your stomach feel swelling and makes a lot of farts because the seaweed breaks down the waste accumulated in your stomach and barrier.

Including distilled water 250 ~ 350ml and incubated at room temperature by using a bubble generator to separate each single strain, and the isolated strains were cultured for 1 week at 25 ~ 35 ℃ each using nutrient agar and blood medium When it appears, subculture three to four times (subculture, 繼 代 培養) to separate.

Single strain separation includes 250 ~ 350ml of distilled water and incubated at room temperature with a bubble generator to separate single strains.

The reason for containing 250 ~ 350ml of distilled water is mixed with distilled water because the distilled water powder, rice bran powder, etc. are mixed after the dilution step to increase the density of the diluent. If the amount is less than 250ml, it is difficult to maintain the uniformity of the spawn culture due to the high density, and if it is more than 350ml, the uniformity of the spawn culture is difficult to maintain.

Since the bubble generator is cultured at room temperature, it is configured to maintain uniform conditions for cultivation, and due to the bubble injury, vortices are generated in the area to increase cultivation efficiency by increasing the contact frequency of microorganisms, and install micro bubbles in the water surface. The device suppresses phytoplankton and removes algae quickly by removing nitrogen.

In addition, subculture is a method of transferring cells to a new culture plate for cell proliferation and continually culturing the cells, which prevents the cells from proliferating in a limited culture dish. It provides a new space for cells to proliferate. The cells are removed from the animal and cultured in a culture dish or test tube. When the cells are cultured in the petri dish, the cells grow as a layer on the bottom of the petri dish. As cells continue to divide in this state, they no longer have enough space to grow on the culture dish and stop proliferating. To allow these cells to continue to proliferate, some of the cells must be removed from the culture dish and transferred to a new culture dish, which is called subculture.

The microorganisms used in the present invention, i.e., some strains are aerobic bacillus positive in gram staining and calcium hydroxide test, positive in catalase test and oxyase test, form resistant spores, and some strains are enterobacteriaceae as anaerobic lactic acid bacteria. It stimulates intestinal immunity, reduces intestinal gas and harmful gas, decreases the amount of COD, increases weight gain and improves meat quality and decreases the total cholesterol content of blood.

In addition, the useful microbial mixed strain of the present invention may be mixed with its culture, extract extracts thereof, formulated into powder pellets, granules, or liquids, and used as an antibiotic-free microbial agent for preventing and controlling animal and fish diseases. As a vegetable, various herbal medicines, plants, and minerals such as edible ground water, seaweed, rice bran, soybean meal, sesame seed, injin, shiitake, rice, white lotus, sakuracho (lineweed), aloe can be used as raw materials. Antibiotic microbial agents can be used for the prevention and prevention of growth, growth inhibition, and death of diseases caused by bacteria, fungi, viruses or diseases caused by infections in animals and plants.

In the first cultivation step, the microorganism spawn 500 ~ 700cc extracted from the above, based on 1t of edible ground water, 250 ~ 350kg of seaweed and 100 ~ 200kg of shiitake mushroom powder are mixed and operated with a bubbler to supply oxygen at room temperature for 2 ~ 4 months. Incubate.

Since the bubble generator is cultured at room temperature, it is configured to maintain uniform conditions for cultivation, and due to the bubble injury, vortices are generated in the area to increase cultivation efficiency by increasing the contact frequency of microorganisms, and install micro bubbles in the water surface. The device suppresses phytoplankton and removes algae quickly by removing nitrogen.

The bubble device can prevent pollutants by dissolution suppression and self-cleaning by supplying a lot of oxygen evenly to the upper and lower parts of the mixture.It also breaks down the organic matter by breaking the air finely by forced aeration circulation in water, so it suppresses and kills algae growth. It is effective in water treatment with

In the second culture step, put 250 ml of Injin mugwort, 150-250 kg of ginger, 50-150 kg of ginger, and 25-75 kg of garlic in the matured liquid phase and continue culturing at room temperature for 1-2 months.

In the aging step, the cultured liquid phase is aged for 1 to 2 months.

In addition, weak buckwheat is also known as Chinese herbal medicine, nectar, eoseongcho, ten pills, the ingredient is raw oil contains 0.005% essential oils. Its main components are methylnonyl ketone CH _3- (CH ₂ ) ₈ -CO-CH ₃ (presumed to be the oxidation product of decanoylacetaldehyde), myrcene C ₁₀ H ₁₆ , laurinaldehyde C ₁₁ H ₂₃ CHO, caprine Aldehyde C ₉ H ₁₉ CHO, capric acid and 28 other components were found by gas chromatography.

The unpleasant odor of this plant is due to decanoylacetaldehyde CH _3- (CH ₂ ) ₈ -CO ₂ -CHO and laurinaldehyde. These two components are found only in fresh plants, and the essential oils obtained by steam distillation include methylnonylketone, myrcene and capric acid.

The leaves contain 2.7% of quercitrin and inorganic loosening. The main component is potassium salt. There are no quercitrins in the flowers and fruit ears, but there is isocurcitrine.

The effect of weak wheat is to reduce the pupils and enlarge the pigmented cells of the skin when subcutaneous injection of 10% brine extract of the outpost to the frog. It also stops the extraction and exposure of frogs, and temporarily contracts the glomerular or peritoneal blood vessels of the kidneys, expanding and paralyzing the rhabdomyomus. In cats, blood pressure is slightly lowered and Mormot's pups and intestinal movements are accelerated.

In addition, perennials are also called joules, perennial perennials. It is about 2 meters high, and leaves are narrow lanceolate and clustered. In August-September, the light yellow female flower grows in the upper part, the red purple male flower in the inflorescence in the lower part, and the fruit bears the fruit. Fruits and young shoots are edible and leaves are used to make salamanders, awnings, and spots. It grows in ponds and watersides and is distributed in Korea, Japan, China, and East Siberia.

The content of the grass is 1.2% protein, 0.1% oil, 0.5% ash in the leaves, protein, starch, sugar in the seeds, its action is used as an antipyretic diuretic to even the intestines and stomach and stop diarrhea.

In addition, the components of shiitake are crude protein 15.3% crude fat 1.0%, crude fiber 10.8% soluble nitrogen-free 63.8%, ash 4.3%, ergosterol vitamin B2, lenthionine, and its effect is that It is used as an anticancer agent because it has an activation activity.

In addition, the main component of garlic consists of allicin, a degradation product of allicin, and divinyl sulfide diallyl sulfide polysulfide, ascorbic acid, scordinin A, glutamic acid, and tripeptide, which are reduced raw Seoul. : Antimicrobial activity against Staphylococcus aureus, Streptococcus, Elysium, and Cholera at a concentration of 125,000. Tift kills within 5 minutes. Also, it inhibits and kills various pathogenic bacteria.

In addition, the components of leeks are propylmercaptan, provitamin A, vitamin B, C, phytin, phytonide, and are used as antiperspirant, cough medicine, antiparasitic medicine and cold medicine.

1. Isolation of Useful Microbial Strains

1 g of the defoliated soil and 50 ml of water taken from the intake were suspended in 10 ml of 0.9% physiological saline. The suspended solids were removed from the supernatant, and the supernatant was diluted 1:10 with saline. Repeat dilution of the dilution three times again, take 1ml of the final dilution, and include 1g of rice bran powder, 0.1g of seaweed powder, 300ml of distilled water, and incubated at room temperature (20 ~ 30 ℃) using a bubble generator to separate each strain. It was.

The isolated strains were cultured for one week at 30 ° C. using nutrient agar medium and blood medium, and when single strains appeared, they were subcultured three to four times.

2. Culture and Ripening

① Fill the bucket with 1t of edible ground water, mix the microorganism spawn (600) cc, seaweed (300) kg, and shiitake mushroom powder (150) kg with the above-mentioned extract and operate the bubbler to supply oxygen. Incubate at 25-30).

② Put injin mugwort (300) kg, green onion (200) kg ginger (100) kg garlic (50) kg barb (15) kg fish vinegar (10) kg in the cultured culture medium, and continued the culture in the same manner as above 1 month Incubate.

③ The cultured liquid is aged for 1 month until the induction stabilized in a state of removing the bubble generator.

Scattering rate, mortality rate of farm using feed additive using composition of the present invention Henhouse (standard) Hanbun Farm (invention) Spawn rate
(July 22 ~ July 28, 2005 / Week 36) 91.5% 94.7% Mortality
(May 6 ~ July 28, 2005 / Volume 25 ~ 36) 6% -10% none  Choosing due to fan collision 2.98 daily averages over 12 weeks
* Week 1.35
Week 1.36 * 7/22 ~ 7/28
Weekly Cheongju Region Highest Temperature
7/22 (33.7 degrees) 7/23 (34.0 degrees) 7/24 (32.3 degrees) 7/25 (31.5 degrees) 7/26 (32.2 degrees) 7/27 (30.9 degrees)
7/28 (26.6 degrees)

The useful microbial mixed strain of the present invention may be mixed with its culture, its extract tin, and formulated into powder pellets, granules, or liquids to prevent and control animal and fish diseases, and can be used as an antibiotic free microbial agent. It is possible to use various herbal medicines, plants, and minerals as edible groundwater, seaweed, rice bran, soybean meal, bean curd, injin, shiitake, rice, white lotus, sakuracho (Julp) fish herb, and aloe.

The present invention induces suicide by shaking the identity of bacterial groups (viruses, harmful bacteria, cells, etc.) in the human body's program and at the opposite point, and implements a new function as a human-friendly program for life in a variable category. By shifting the growth program of the living body, it has the function of capturing and disassembling it to the body, including toxin, saturated fat, etc., which is a point of contrast to the program that brings cellular activity, promotes cell regeneration, and maintains the flora and fauna.

The composition of the present invention as described above can be used as a non-antibiotic feed additive and a pesticide-free soil improver composition.

In the field of application, the agricultural field improves the crop yield through soil improvement as a land improving agent using powder, cultivation of pesticide-free crops, high functional crop cultivation with bio-information, and in the livestock fishery field, the composition of the present invention It is composed of feed additives, and it is possible to raise antibiotic-free cattle, pigs, chickens, non-antibiotics, non-hormonal milk production, and antibiotic-free fish farming.

Claims (3)

Suspension step of removing suspended solids in the upper layer after suspending 1 ~ 2g and 1 ~ 2g of water and 45 ~ 55ml of physiological saline;
Taking a supernatant from the suspended mixture, diluting with 1:10 with physiological saline, and diluting again 2-4 times;
1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of distilled water, 250 ~ 350ml of distilled water based on the final dilution 1ml of the dilution step and incubated at room temperature with a bubble generator to separate each single strain, separated Spawn culture step of culturing strains at 25-35 ° C. for one week using nutrient agar medium and blood medium to separate three to four times when subcultured (subculture, 繼 代 培養) when a single strain appears;
Based on the 1t of edible ground water, 500 ~ 700cc of the microorganism spawn extracted above, 250 ~ 350kg of seaweed and 100 ~ 200kg of shiitake mushrooms are mixed and operated with a bubbler to supply oxygen to incubate at room temperature for 2 ~ 4 months. step;
250-350kg jinjin wormwood, 150-250kg green onions, 50-150kg ginger, 25-75kg garlic in a mature culture liquid, the second culture step of continuing the culture at room temperature for 1-2 months;
1 to 2 months of culturing the cultured liquid; aging step; characterized in that formed through seaweed, rice bran, manjin, shii, leek, garlic, ginger, flora and fish diseases composition for prevention and control, treatment Manufacturing method. Suspension 1 ~ 2g and 45 ~ 55ml of water in 10ml of physiological saline, suspended in 10ml of physiological saline, suspended in the upper portion, take the supernatant from the suspension mixture and diluted 1:10 with physiological saline. Diluting again and again 2-4 times, containing 1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of seaweed powder, 250 ~ 350ml of distilled water based on 1ml of the final dilution solution after the dilution step and incubated at room temperature by using a bubble generator Each isolate is isolated by culturing a single strain at 25-35 ℃ for one week using nutrient agar and blood medium, and then subcultured three to four times when a single strain appears. Spawn culture, mixed with 500 ~ 700cc microbial spawn and 250 ~ 350kg of seaweed, 100 ~ 200kg shiitake mushroom powder extracted from the above based on 1t of edible ground water, and operating a bubbler to supply oxygen 1 ~ 2 months at room temperature Tea times Yangjin 250 ~ 350kg, leek 150 ~ 250kg, ginger 50 ~ 150kg, garlic 25 ~ 75kg in a culture medium, matured in the culture medium, and the second culture at room temperature for 1-2 months, and the cultured liquid for 1-2 months Composition and feed additives related to the prevention, control and treatment of animal and fish diseases using seaweed, rice bran, jinjin, shiitake, leek, garlic and ginger. Suspension 1 ~ 2g and 45 ~ 55ml of water in 10ml of physiological saline, suspended in 10ml of physiological saline, suspended in the upper portion, take the supernatant from the suspension mixture and diluted 1:10 with physiological saline. Diluting again and again 2-4 times, containing 1 ~ 2g of rice bran powder, 0.1 ~ 0.2g of seaweed powder, 250 ~ 350ml of distilled water based on 1ml of the final dilution solution after the dilution step and incubated at room temperature by using a bubble generator Each isolate is isolated by culturing a single strain at 25-35 ℃ for one week using nutrient agar and blood medium, and then subcultured three to four times when a single strain appears. Spawn culture, mixed with 500 ~ 700cc microbial spawn and 250 ~ 350kg of seaweed, 100 ~ 200kg shiitake mushroom powder extracted from the above based on 1t of edible ground water, and operating a bubbler to supply oxygen 1 ~ 2 months at room temperature Tea times Yangjin 250 ~ 350kg, leek 150 ~ 250kg, ginger 50 ~ 150kg, garlic 25 ~ 75kg in a culture medium, cultivated in a culture medium, and the second culture at room temperature for 1-2 months, the cultured liquid for about 1-2 months Soil improver using seaweed, rice bran, jinjin, shiitake, green onion, garlic and ginger, which are formed by powdering the composition formed by aging.