KR20120082744A - Cosmetic composition - Google Patents

Abstract

PURPOSE: A cosmetic composition containing glycose aminoglycan and Centella asiatica (L.) ubain extract is provided to improve collagen and hyaluronic acid synthesis and anti-wrinkling effect. CONSTITUTION: A cosmetic composition contains 1.5-15.0 weight parts of glycose amino glycan isolated from snails, and 0.01-10 weight parts of Centella asiatica (L.) ubain extract. The glycose amino glycan and Centella asiatica (L.) ubain extract are added to a skin softener, nutrition lotion, massage cream, essence, or pack.

Description

Cosmetic composition for skin protection {Cosmetic Composition}

The present invention relates to a cosmetic composition for protecting the skin, and more particularly, to a moisturizing cosmetic composition for preventing skin drying having glycosaminoglycans and centella extracts extracted from the mucus of the snail and having antibacterial, moisturizing and antioxidant effects. .

The skin consists of several layers of cells that coat and protect keratin and collagen fiber proteins that form the framework of the skin structure. Dermatologically, the most important role of the skin is to act as a barrier to prevent the loss of biological components such as water and electrolytes and to prevent the invasion of harmful substances into the skin under the external environment. In other words, the skin is exposed to a much drier external environment than in vivo and plays an important role as a membrane to protect the living body from the external environment. In particular, the stratum corneum of the skin's outermost layer, which is in direct contact with the external environment, prevents the invasion of mechanically and chemically harmful ingredients, and prevents the loss of internal moisture in the dry environment. Is keeping.

However, despite this function of the skin, the skin is easily exposed to harsh external environment, such as wind, cold weather, sunshine, face wash, shaving, etc., with aging factor, which easily loses moisture and becomes dry and rough skin.

Therefore, to prevent this phenomenon to maintain a smooth and moist skin is the most important purpose of using cosmetics. In other words, applying cosmetics to the skin creates an artificial membrane on the skin that prevents external irritation, prevents water loss from inside the skin, and supplies some amount of moisture to the skin.

However, the conventional cosmetics having such a general moisturizing action is smooth and moist, it is not easy to do skin care without burden on the skin.

On the other hand, the main cause of skin aging is the lack of estrogen (estrogen). The main function of the female hormone estrogen on the skin is known to enhance skin activity and make the skin soft, elastic and supple. Specifically, estrogen stimulates fibroblasts in the dermis to promote collagen synthesis, is involved in collagen metabolism, inhibits over-glycation of collagen, and regulates the expression of collagenase, a collagen degrading enzyme, Suppresses decomposition of In addition, by increasing the proliferative effect of the basal vesicles on the epidermis, the surface becomes thicker, thereby strengthening the skin tissue and regulating the sebaceous glands to inhibit the growth of sebaceous glands and to reduce sebum production, Promote synthesis

If the estrogen is deficient in this effect is caused by the change of the skin, the typical phenomenon is the skin drying. In fibroblasts, the production of collagen, elastic fibers, etc. is suppressed, resulting in thinning of the skin, decreasing the elasticity of the skin, and inhibiting the synthesis of hyaluronic acid, thereby weakening the skin's water retention function. In addition, the increase in collagen glycation increases the cross-linking of collagen to harden the skin to decrease the elasticity.

However, the existing cosmetics did not directly deal with estrogen deficiency, but indirect treatment using general inhibitors of skin aging such as moisturizers, anti-aging agents, free radical scavengers, and sunscreens.

The present invention, in order to solve the above-mentioned problems, glycosaminoglycans extracted from the snail mucosa, which is an excellent moisturizing agent and improves the water retention ability, and centella asiatica extract showing excellent cell proliferation effect, collagen synthesis effect and hyaluronic acid synthesis effect. An object of the present invention is to provide a cosmetic composition for protecting skin.

      In order to achieve the above object, the cosmetic composition for skin protection of the present invention is characterized in that it contains 1.5 to 15.0 parts by weight of glycosaminoglycans extracted from snail mucus and 0.01 to 10 parts by weight of centella extract.

In addition, the cosmetic composition for skin protection of the present invention is characterized in that it contains 1.0 to 10.0 parts by weight of glycosaminoglycans extracted from snail mucus and 0.01 to 10 parts by weight of centella extract.

In addition, the glycosaminoglycans and the centella extract in the cosmetic composition for skin protection of the present invention is characterized in that it is added to any one of the basic cosmetics selected from softening water, nutrient cosmetics, massage cream, essence, pack.

The cosmetic composition according to the present invention has excellent skin moisture retention ability by containing glycosaminoglycans extracted from snails, and has excellent cell proliferation effect, collagen and hyaluronic acid synthesis effect, wrinkle improvement effect by containing centella extract. By containing the ingredients within the most preferred dosage range it has an excellent effect on skin protection.

      The sticky mucus of the snail focuses on keeping the skin always moist and moist even in dry weather, so that glycosaminoglycans extracted from the mucus tissue of fresh snails are used.

      Glycosaminoglycans are present in most animals as a mixture of mucopolysaccharides, but in very small amounts they are contained in mucosa of snails.

      In the manufacture of a moisturizing cosmetic for preventing skin drying, the content of glycosaminoglycans extracted from the mucus of the snail as a moisturizing agent is 1.0 to 20.0 parts by weight, preferably 1.5 to 15.0 parts by weight. At 1.0 parts by weight or less, it is difficult to expect water retention capacity, and at 20.0 parts by weight or more, it is uneconomical.

      Hereinafter, the present invention will be described in more detail by way of examples.

Example

In order to find out the skin moisture retention capacity, skin moisture evaporation inhibitory ability of the cosmetic composition prepared in Examples and Comparative Examples was tested as follows.

The pigments, preservatives, water-soluble polymers, nonionic surfactants, chemicals, higher fatty alcohols and oils and fats contained in the examples of the present invention are raw materials widely used in conventional cosmetics within the scope that does not affect the effect claimed in the present invention, and The content was used.

Examples 1-3, Comparative Examples 1-9

Moisture retention test for moisturizing substances

Next, the oily ingredients were heated and dissolved in the basic formulations as shown in Table 1, and the oily ingredients and the neutralizing agent were gradually added to the aqueous phase ingredients, stirred, and cooled.

In order to evaluate the moisturizing power of the cosmetic composition prepared according to Examples 1 to 3 and Comparative Examples 1 to 9, after applying the cosmetic composition prepared according to Examples 1 to 3 and Comparative Examples 1 to 9 to the skin, The method of measuring skin moisture retention ability by measuring conductivity was used.

In the test method, the cosmetic composition prepared according to Examples 1 to 3 and Comparative Examples 1 to 9 was applied to 30 test subjects in a predetermined amount, rubbed well, and the moisture content of the skin was measured 2 hours after the application.

As a measuring device, a skin moisture content measuring device for measuring the electrical conductivity of the skin was used.

division
Raw material name
content
Oily ingredients
Cetostearyl alcohol

Mineral Oil

Monostearic acid polyoxyethane sorbitol

Sesquioleate sorbitan 2.0

10.0

0.7

0.5
Water component
Purifier

Carboxy Vinyl Polymer 78.6

0.1


Moisturizing Enhancer
8.0
corrector

Triethanolamine
0.1

Moisturizing substances
Content (parts by weight) Skin conductivity after 2 hours of application
Example 1
Glycosaminoglycans
0.8
21.9
Example 2
Glycosaminoglycans
8.0
48.2
Example 3
Glycosaminoglycans
17.0
52.7
Comparative Example 1
Hyaluronic acid
8.0
10.8
Comparative Example 2
Sodium pyridone carboxylate
8.0
2.5
Comparative Example 3
Sodium lactate
8.0
7.9
Comparative Example 4
amino acid
8.0
3.6
Comparative Example 5
Collagen
8.0
2.0
Comparative Example 6
Chitin liquid
8.0
5.0
Comparative Example 7
glycerin
8.0
32
Comparative Example 8
Sorbitol
8.0
20.5
Comparative Example 9
1,3-butylene glycol
8.0
14.9

As shown in Table 2, Examples 1 to 3 based on the present invention showed much higher electrical conductivity of the skin after 2 hours of application than Comparative Examples 1 to 9, and thus, the Examples were much better than the Comparative Examples. It can be seen that.

Example 4

Next, the oily phase component and the aqueous phase component were heated and dissolved in the composition as shown in Table 3, and then the oily phase component was slowly added to the aqueous phase component, stirred, and cooled to prepare a moisturizing cream.

division
Raw material name
Content (unit weight part)

Oily ingredients

Cetostearyl alcohol

Stearic acid

Cholesteryl ester

Liquid paraffin

Octyldodecanol

Glyceryl Monostearate

Propylparaben

Ceramide

Cerebroside 5.0

3.0

5.0

15.0

4.0

3.0

0.1

0.1

0.1

Water component

Purified water

Glycosaminoglycans

Methylparaben

Potassium hydroxide 54.3

10.0

0.2

0.2

 Comparative Example 10

Next, the oily phase component and the aqueous phase component were dissolved in the composition as shown in Table 4, and then the oily phase component was slowly added to the aqueous phase component, stirred, and cooled to prepare a moisturizing cream.

division
Raw material name
Content (unit weight part)

Oily ingredients

Cetostearyl alcohol

Stearic acid

Vaseline

Liquid paraffin

Octyldodecanol

Glyceryl Monostearate

Propylparaben 5.0

3.0

5.0

15.0

4.0

3.0

0.1



Water component

Purified water

glycerin

Propylene glycol

Methylparaben

Potassium hydroxide 54.5

5.0

5.2

0.2

0.2

Moisture retention test

In order to evaluate the moisturizing power of the cosmetic composition prepared according to Example 4 and Comparative Example 10, a water retention test was performed.

The cosmetic compositions prepared according to Example 4 and Comparative Example 10 were applied to 30 test subjects, respectively, and the moisture content of the skin was measured before application, after 1 hour of application, and after 2 hours of application.

product
Example 4
Comparative Example 10
Skin electrical conductivity before application
6
6
Skin electrical conductivity 1 hour after application

43
15
Skin electrical conductivity 2 hours after application
37
10

As shown in Table 5, the cosmetic composition prepared according to Example 4 showed much higher skin conductivity after 1 hour and 2 hours than the cosmetic composition prepared according to Comparative Example 10, and it was confirmed that the moisture retention ability was much better.

Water evaporation test

A water vaporization test was conducted to evaluate the moisturizing power of the cosmetic composition prepared according to Example 4 and Comparative Example 10.

Each of the cosmetic compositions prepared according to Example 4 and Comparative Example 10 was applied to 30 test subjects, rubbed, and the amount of water evaporation of the skin was measured using a water vaporizer for 10 minutes at each 2 minute interval.

product
Example 4
Comparative Example 10
Water evaporation after 10 minutes
29%
42%

As shown in Table 6, the cosmetic composition prepared according to Example 4 exhibited much less water evaporation than the cosmetic composition prepared according to Comparative Example 10.

Next, the cosmetic composition of the present invention is characterized by containing the Centella as an active ingredient extract of 0.0005 to 10.0 weight and more preferably 0.01 to 5 weight based on the total dry weight of the cosmetic composition. If the content is less than 0.0005% by weight, no obvious effect can be expected. If the content is 10.0% by weight or more, the apparent effect due to the weight of the content does not increase.

Centella is a perennial plant belonging to the Buttercup family, called horseshoe grass and clam grass. It is native to Madagasca Island in eastern Africa and is thought to be effective for antipyretics, skin ulcers, trauma, tuberculosis and alopecia areata.

The Centella asiatica extract of the present invention is extracted into centellae with water of 1 to 10 times its dry weight, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform and 1,3-butylene glycol, The extract is distilled off under reduced pressure, followed by freeze drying or spray drying.

Centella asiatica extract of the present invention can be added to the basic cosmetics, such as softening water, nutrient cosmetics, massage cream, essence, pack according to a conventional method can be used as a cosmetic for preventing and improving skin aging.

Example

The centella was washed with purified water and dried. Then, 200 grams of powdered centella pollen was added to 2.5 L of 70% ethanol extract, boiled for 4 hours in a reflux extractor equipped with a cooling condenser, filtered with 300 mesh filter cloth, and the residue was filtered in the same manner. Extracted once more. Each extract was combined, filtered through Whatman No. 5 filter paper, and the solvent was removed using a vacuum concentrator, and lyophilized to obtain a dry weight of 51.4 grams.

Soft cosmetics (skin) in cosmetics containing Centella asiatic extract obtained by the above method was prepared.

 ingredient  Content (% by weight)  Centella asiatica extract  0.2  1,3-butylene glycol  6.0  glycerin  4.0  Oleyl alcohol  1.2  Polysorbate20  3.0  ethanol  5.0  Propylparaben  0.1  Benzophenone-9  2.0  Carboxyvinyl polymer  1.0  Pf.962  0.2  Purified water  Balance  system  100

Experimental Example 1. Cell Proliferation Effect of Centella asiatica

Human normal fibroblasts were incubated for 24 hours to 1 * 10 ⁴ cells in each well of a 96-well microplate, and the centella extract prepared above was replaced with serum-free DM medium adjusted to a final concentration of 250 μg / ml. After that, the cells were further incubated for 24 hours. In Table 8, the control group was cultured without adding centella extract. 100 μl of dimethyl sulfoxide solution was added to each well, followed by stirring for 20 minutes, and the absorbance was measured at 500 nm with a microplate reader.

extract
Absorbance (500 nm)
Cell proliferation effect (%)
Control group
0.365
-
Example
0.565
54.6

From the above results, it can be seen that the Centella asiatica extract shows an excellent taxation effect.

Experimental Example 2. Enhancement of Collagen Synthesis by Screen Extract

Human normal fibroblasts were inoculated in 96-well microplates (2 * 10 ⁴ cells in each well) and incubated for 24 hours, and centrifugal extracts of the concentrations shown in Table 9 were added together with the medium and incubated for 48 hours. In Table 9, the control group was cultured without adding centella extract. Ascorbic acid was added to promote collagen synthesis before the last 24 hours of culture. After incubation, each well was washed, replaced with DM medium without serum, and further incubated for 24 hours. After incubation, the supernatant of each well was collected and the amount of procollagen type IC-pitide was measured by the amount of newly synthesized collagen using the kit.

density
Collagen synthesis amount (ng / 2 * 10 ⁴ )
Control group
153
50 µg
198
100 µg
245
200 µg
291

From the above results, it can be seen that the inclusion of the centellar extract shows a superior collagen synthesis promoting effect, compared to the control group does not contain the centellar extract, and the synthesis of collagen increases as the centellar extract concentration increases.

Experimental Example 3. Wrinkle improvement

Five hairless mice, 6 weeks old, were used to measure the wrinkle improvement effect of Centella asiatica on light induced wrinkles. Centella asiatica extract was applied by dissolving the centella asiatica extract prepared in Example in distilled water, and the test method is as follows.

The hairless mouse was irradiated with 2MED for 3 days a week for 10 weeks using a solar tube stimulator to form wrinkles, and the control group treated with distilled water without adding a sample and 0.5% extract 0.5m / cm 3 twice a day. The degree of improvement was determined in the group treated for 6 weeks.

Wrinkle improvement was judged by visual observation and photographing of the sample treatment area first, and the criterion was no improvement, slight improvement, moderate improvement, and significant improvement compared to each extract treatment group and control group before the sample treatment. It was determined in four steps of Table 10 is shown.

No improvement
Minor improvement
Secondary improvement
Considerable improvement
Control group
5
0
0
0
Centella extract treatment of 0.5% Example
0
0
3
2

From the above results, Centella aspergillus extract can be seen that there is an excellent improvement effect on the wrinkles generated by the light of the hairless mouse.

As a result, the glycosaminoglycans extracted from the snail mucus were proved to have an excellent effect on water retention and skin moisture evaporation inhibitory effect. You can check it.

Thus, when the glycosaminoglycans and centella extracts, which are useful for skin protection, are mixed in a dose within the most desirable range, the most desirable effect can be expected.

Claims (3)

A cosmetic composition comprising 1.5-15.0 parts by weight of glycosaminoglycans extracted from snail mucus and 0.01-10 parts by weight of centella extract.
A cosmetic composition comprising 1.0-10.0 parts by weight of glycosaminoglycans extracted from snail mucus and 0.01-10 parts by weight of centella extract.
The cosmetic composition according to claim 1, wherein the glycosaminoglycans and the centella extract are added to any one of the basic cosmetics selected from softening water, nutrient cream, massage cream, essence and pack.