KR20110068203A - Cosmetic composition for controlling anti-acne and anti-comed - Google Patents

Abstract

PURPOSE: A cosmetic composition containing bamboo sap for treating acne is provided to ensure antibacterial activity against Propionibacterium acnes and to prevent and relieve inflammation. CONSTITUTION: A cosmetic composition for treating acne contains bamboo sap. The composition is used for controlling sebum secretion and reducing comedon generation. A method for manufacturing the composition comprises: a step of collecting bamboo sap and filtering the sap; a step of adding 7.0 weight% or more of anhydrous alcohol for pretreatment; a step of removing proteins with large molecular weight; and a step of adding anhydrous alcohol.

Description

Cosmetic composition for improving acne skin {Cosmetic composition for controlling anti-acne and anti-comed}

The present invention relates to a cosmetic composition for improving acne skin by containing bamboo sap, having an antibacterial effect against acne causing bacteria, sebum secretion inhibitory effect and anti-inflammatory effect.

Acne is an inflammatory disease of the fur branch, hair follicles and surrounding tissues caused by hormone action, excessive sebum secretion, bacterial infections, and the like.

The causes of acne can be divided into several ways. The first cause is excessive secretion of sebum. Sebum is a substance secreted by the sebaceous glands, and in normal amounts it acts to prevent harmful substances outside the skin from penetrating keratinocytes. However, under puberty, before and after menstruation, early pregnancy, contraceptives, and stress, the human body shows a different hormone balance than normal. This altered hormonal balance stimulates the sebaceous glands, which leads to an oversecretion of sebum. In addition, sebum secretion may be genetically increased and may be increased by changes in external temperature or skin temperature caused by poor eating habits.

The second cause is infection of the pores. Excessively secreted sebum contains a lot of fatty acids to increase the pH of the skin surface, dissolving the lipids of the skin lowers the defense of the skin itself. On the surface of the skin with high pH, acne-causing bacteria become easy to multiply, and the multiplying acne bacteria cause purulent inflammation through weakened skin.

In general, acne can be divided into four stages according to symptoms. The first stage is scleroderma acne, in which excessive secretion of sebum accumulates in the pores around the sebaceous glands, forming a scalp. Stage 1 spontaneous acne is an early stage of acne lesions but cosmetically not good skin trouble. Good care at this stage can also inhibit the development of inflammation. Stage 2 Papular Acne The acne germs multiply and cause inflammatory reactions that cause red acne, which is called papules and can cause scars if not properly managed. In the third stage purulent acne, the inflammatory reaction of red acne becomes more severe, and pus inside is formed. This is called pustules. In this stage, scars may remain even after treatment, so professional treatment is required. The final stage 4 nodular acne is a stage in which the pus breaks out of the skin and causes more inflammation around the acne lesion. Can be prolonged.

Treatment of acne includes topical formulations and oral medications, and surgical treatments such as laser and dermabrasion that have recently been performed.

Topical application therapy uses antibacterial agents such as azelaic acid and salicylic acid for the purpose of antibacterial action against propionibacterium acnes, known as the cause of acne, or benzoyl peroxide for dissolving cotton cloth. peroxide) is used as external ointment type. However, azelaic acid is difficult to formulate and salicylic acid is classified as a preservative in Korea, there is a problem that can not contain more than 0.5% in the formulation. In the case of benzoyl peroxide, there is a problem that it cannot be used as a cosmetic raw material in Korea.

Oral drug therapy uses retinoids and corticosteroids to inhibit sebaceous gland function and inhibit hair follicle wall destruction. These preparations have side effects such as skin rash, dry skin, and allergies may occur depending on the individual.

The problem of already developed, advanced acne and other skin diseases is that the external preparations used directly on the skin are more effective considering various aspects such as the speed of the effect and the economics.

There are not many substances that can see these medical effects at the cosmetic stage. However, it can be used for a long time and has less damage to normal skin than a therapeutic drug. Thus, many plant extracts and synthetic materials have been patented as a composition for improving acne skin, but there are not many products that have a therapeutic effect such as skin exfoliation after use and high skin side effects, which are safe for the skin and show the effect desired by consumers.

The present invention has the effect of controlling the sebum secretion essential for acne improvement as well as the antibacterial effect against acne causing bacteria by containing bamboo sap, and has the effect of improving acne in a wide range of progression, cosmetic composition for acne skin improvement To provide that purpose.

The cosmetic composition for improving acne skin according to the present invention is characterized by containing bamboo sap.

In addition, the cosmetic composition for improving acne skin according to the present invention is characterized in that the use of antimicrobial propionibacterium acnes, which is the cause of acne, the use of controlling sebum secretion, the use of reducing scleroderma, and anti-inflammatory. do.

The cosmetic composition for improving acne skin according to the present invention primarily exhibits its effect by rapidly inhibiting the growth and growth of microorganisms involved in skin acne development and skin inflammation, and secondly, an excess of one of the causes of acne. Improves acne skin by inhibiting sebum secretion and extinguishing vaginal acne. It also has an anti-inflammatory effect, which improves acne skin.

The cosmetic composition for improving acne of the present invention comprises bamboo sap.

Bamboo sap used in the present invention is produced as a by-product while periodically cutting to help the growth in the bamboo cultivation process, and the composition ratio of the composition is different depending on the environmental factors such as the cultivation area, the species of bamboo, the harvesting time It contains protein (0.08 ~ 0.7%), calcium (422mg / l), sugar (1.6 ~ 2.7g / l), amino acid (7.6 ~ 30mg / l), and the pH is about 4.5. .

Meanwhile, in the pretreatment method, the bamboo sap immediately after collection is filtered with a filtration membrane having a pore size of 0.001 to 0.45 μm, preferably a pore size of 0.2 μm, and then anhydrous ethanol is added to the filtrate in an amount of 7.0% by weight or more. Preferably, it is added in the quantity of 7.0-50 weight%. At this time, the size of the filtration membrane is limited to 0.001 ~ 0.45㎛ because when the size of the filtration membrane is less than 0.001㎛ takes a long time to take the filtration, there is a problem that the filtration efficiency is less effective than 0.45㎛.

In the present invention, the bamboo sap can be used directly, or pre-treatment may be used as an active ingredient so as not to discolor and deodorize on storage before use.

In other words, by filtration through a filter membrane, it is used as a nutrient for bacteria, microorganisms and microorganisms in bamboo sap, or a protein having a high molecular weight, which may be precipitated or denatured during long-term storage, is removed. Inhibition prevents discoloration and odor.

The pretreated bamboo sap is sealed directly under nitrogen atmosphere to prevent invasion of external bacteria or microorganisms, and then opened and used in actual use.

The content of the bamboo sap in the external base may be appropriately selected depending on the formulation, and may be selected within the range of 0.01 to 99.5% by weight, preferably 1.0 to 90.0% by weight, based on the total weight of the composition.

The external skin base containing the pretreated bamboo sap of the present invention is a cosmetic having a formulation such as softener, nourishing cosmetic, massage cream, nutrition cream, pack, gel or skin adhesive type cosmetic, lipstick, makeup base, foundation, etc. And transdermal dosage forms such as shampoos, rinses, body cleansers and soaps, lotions, ointments, gels, creams, patches or sprays.

Although the present invention will be described in more detail with reference to Examples and Test Examples of the present invention, the present invention is not limited only to these examples.

<Test Example 1> Pretreatment Method

The first or second node was cut from the roots of bamboos grown for more than five years, and the sap that was raised from the roots was collected by using a connection tube closely attached to the cuts. The bamboo sap immediately after collection was filtered with a pore size of 0.2 μm.

After passing, the treatment was carried out by the method of Table 1 below. Among them, DM and DP were dissolved so that the content of DM and DP in ethanol was 10.0% by weight, and the bamboo sap was collected so that 3 g of basis weight was 100 g, followed by room temperature, 45 ° C. and refrigerated storage (4 ° C.). The preservative effect was confirmed with progress. As a result, when 7.0% by weight of anhydrous ethanol it was confirmed that the antiseptic effect until 30 days.

Therefore, when the bamboo sap is treated with 7.0% by weight or more of anhydrous ethanol, it is prevented from discoloration and odor, and can be effectively used as a raw material for cosmetics and the like.

The formulation of the acne improvement cosmetic composition comprising the bamboo sap of the present invention as an active ingredient is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in the form of external skin ointment, softening longevity, nourishing longevity, nutrition cream, massage cream, essence, pack, emulsion, oil gel and the like. At this time, the external skin ointment may be prepared to contain 50 to 97% by weight of petrolatum and 0.1 to 5% by weight of polyoxyethylene oleyl-ether phosphate in addition to the herbal fermentation extract of the present invention, the flexible cosmetic water is bamboo sap of the present invention In addition, it can be prepared to contain 1 to 10% by weight of polyhydric alcohols (propylene glycol, glycerin, etc.) and 0.05 to 2% by weight of surfactants (polyethylene oleyl ether, polyoxyethylene hydrogenated castor oil, etc.). In addition to the nutrient cream and nutritive cream, in addition to the bamboo sap of the present invention, oils (squalane, petrolatum, octyldodecanol, etc.) 5-20% by weight and wax components (cetanol, stearyl alcohol, beeswax, etc.) 3-15% by weight It can be prepared to contain, and the essence can be prepared by containing 5 to 30% by weight of polyhydric alcohols such as glycerin, propylene glycol in addition to the bamboo sap of the present invention. Massage cream is prepared by containing 30 to 70% by weight of oil, such as liquid paraffin, petrolatum, isononyl isononanoate in addition to the herbal fermentation extract of the present invention, the pack is polyvinyl alcohol 5-20 in addition to the herbal extract of the present invention Wash off containing 5-30% by weight of pigments such as kaolin, talc, zinc oxide and titanium dioxide in addition to the fermented extract of the herbal medicine of the present invention in a peel-off pack or a general emulsion cosmetic containing weight% ) Pack.

In addition, the cosmetic composition for improving acne of the present invention may be formulated into oils, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., which are formulated in general skin cosmetics in addition to the components described above in each formulation. It can mix and use suitably as needed.

<Formulation Example 1 and Comparative Formulation Example 1>

Flexible Cosmetics

ingredient Formulation Example 1
Content (% by weight) Comparative Formulation Example 1
Content (% by weight) Betaine 3.0 3.0 Natto gum 0.08 0.08 Cellulose gum 5.0 5.0 ethanol 0.5 0.5 Polyoxyethylene Cured Castor Oil 0.2 0.2 Tocopherol Acetate 5.0 5.0 antiseptic a very small amount a very small amount Pigment a very small amount a very small amount Pretreated Bamboo Sap to 100 Distilled water - to 100

<Formulation Example 2 and Comparative Formulation Example 2>

Nutrition

ingredient Formulation Example 1
Content (% by weight) Comparative Formulation Example 1
Content (% by weight) 3,7 hexanoate 5.0 5.0 Cetostearyl alcohol 1.0 1.0 Lipophilic Monostearic Acid Stearate 0.8 0.8 Squalane 2.0 2.0 Polysorbate 60 1.5 1.5 Solbitan Sesquioleate 0.5 0.5 glycerin 8.0 8.0 Triethanolamine 0.2 0.2 Carboxy Vinyl Polymer 0.2 0.2 antiseptic a very small amount a very small amount Pigment a very small amount a very small amount incense a very small amount a very small amount Pretreated Bamboo Sap to 100 - Distilled water - to 100

<Formulation Example 3 and Comparative Formulation Example 3>

Nutrition Cream

ingredient Formulation Example 1
Content (% by weight) Comparative Formulation Example 1
Content (% by weight) Beeswax 1.0 1.0 Glyceryl Stearate 2.0 2.0 Cetostearate 2.5 2.5 Polysorbate 60 1.5 1.5 Solbitan Sesquioleate 0.5 0.5 3,7 hexanoate 5.0 5.0 Squalane 5.0 5.0 Liquid paraffin 8.0 8.0 glycerin 8.0 8.0 Propylene glycol 4.0 4.0 Plant extract 5.0 5.0 antiseptic a very small amount a very small amount Pigment a very small amount a very small amount incense a very small amount a very small amount Pretreated Bamboo Sap to 100 - Distilled water - to 100

Experimental Example 1 Antibacterial Activity Test against Acne Bacteria

Antibacterial activity against propionibacterium acnes <ATCC 6919: Medium-BHI broth> having the respective cosmetic composition prepared in the formulation of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 Was tested.

The antibacterial test method for acne bacteria was as follows.

(1) Preparation of test bacteria

Propionibacterium acnes was used as a culture broth inoculated in BHI broth and anaerobic culture.

(2) dilution solution preparation

0.15 ml of the test bacteria was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5) and mixed well as a dilution solution.

(3) Sample preparation

The cosmetic composition stock solutions prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 were used as samples.

(4) antibacterial test

1) Insert the sample to the starting concentration in the first well of 96-well cell culture tube (96well plate) and adjust the total amount to 200ul with the diluted solution. Into the remaining wells add 100ul of diluted solution.

2) Mix the mixed solution of the first row well, take 100ul and add it to the second row, mix well, and then take 100ul again and add it to the third row to double dilution.

3) After standing incubation for 24 hours and 48 hours at 32 ℃, determine the growth of bacteria to the extent of suspension and determine the minimum concentration without growth of bacteria as MIC (Minimun Inhibitory concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.

The antimicrobial activity test results for acne bacteria are shown in Table 2 below.

Item pH Propionibacterium Acnes Formulation Example 1 5.6 <5 ppm Formulation Example 2 5.3 <1 ppm Formulation Example 3 5.2 <5 ppm Comparative Formulation Example 1 5.7 * Cannot be measured Comparative Formulation Example 2 5.5 <10 ppm Comparative Formulation Example 3 5.7 <30 ppm

Formulation Examples 1 to 3 had an antimicrobial activity of 20 ppm to 1 ppm of MIC against the test bacteria. The lower the concentration of ppm in MIC, the more effective the substance against acne.

[Experimental Example 2: improvement of acne and sebum secretion and the presence of irritation]

Twelve people with acne were allowed to use the cosmetic composition prepared from Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 for one month. The acne improvement scale ranged from 1 to 5 points, with 1 being ‘no’, 3 being ‘normal’ and 5 being ‘very very’. The experimental results are expressed in the average score of 12 people in Table 3 below.

Acne disappearance was based on the number of days the extinction was read, acne recurrence was based on the results after one month. Sebum secretion is reduced from 1 to 5 points, with 1 being ‘no’, 3 being ‘normal’ and 5 being ‘very very’. Experimental results are shown in the average score of 12 people in Table 3. The presence or absence of skin irritation was determined as (number of irritants) / (total number of test subjects).

Inflammatory acne
Improving Acousoid Acne
Extinction Acne recurrence Reduce sebum secretion Irritation Formulation Example 1 4.2 5 days radish 4.0 1/12 Formulation Example 2 4.5 2 days radish 4.1 2/12 Formulation Example 3 4.4 5 days radish 4.0 1/12 Comparative Formulation Example 1 2.1 13th U 2.0 0/12 Comparative Formulation Example 2 4.2 2 days radish 4.1 9/12 Comparative Formulation Example 3 3.0 10 days U 2.8 1/12

As shown in Table 3, Formulation Examples 1 to 3 did not have acne recurrence compared to Comparative Formulation Examples 1 and 3, an average of 4 points or more for improving inflammatory acne, 5 days or less for an acne extinction time, sebum An average of 4 points or more was obtained for the reduction of secretion, indicating that there is an excellent effect on acne improvement. On the other hand, Comparative Formulation Example 2 containing an antimicrobial activity standard, showing similar efficacy as Formulation Examples 1 to 3, but the use of the stimulation is stronger than the rest of the other experiments in use, there is concern of skin irritation for long-term use have.

Therefore, it can be seen that the fermented extract of the herbal medicine is suitable for acne skin patients in view of various factors such as irritation, improvement rate, degree of improvement, and degree of sebum secretion.

Experimental Example 3: Inflammation Improvement Effect

The anti-inflammatory effect was evaluated by the inhibitory effect of the production of prostaglandins. The effect was measured on macrophages using the electro-extracted extract. First, aspirin was added to macrophages taken from the abdominal cavity of mice to a final concentration of 500M to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, 100 μl of the suspension was added to each well of a 96-well cell culture tube and incubated for 2 hours in an incubator at 37 ° C. with 5% CO ₂ to attach macrophages to the container surface. The attached macrophages were then washed three times with PBS and used for the effect test of the extract. Incubated for 12 hours by adding RPMI medium containing 1% of LPS (w / v) to 5 × 104 cells / ml of electrocultured macrophages, inducing prostaglandin production and processing 100 μl of the extract to release free Quantification was carried out using an assay (ELISA).

In this case, the production inhibitory activity of the prostaglandin of the extract was set to 100% the difference between the prostaglandin produced in the LPS-treated and untreated groups, respectively, and treated with LPS and the sample to reduce the percentage of prostaglandin The results were compared and determined, and the results (the effect of inhibiting production of prostaglandins) are shown in Table 4 below.

Blank 100% Control group (Aspirin treated group) 25.0% Formulation Example 1 32.8% Formulation Example 2 34.2% Formulation Example 3 39.2% Comparative Formulation Example 1 15.4% Comparative Formulation Example 2 24.2% Comparative Formulation Example 3 25.5%

As shown in Table 4 above, the experimental results show that the inhibitory effect of the production of prostaglandins according to Formulation Examples 1 to 3 is significantly higher than that of the control group treated with aspirin, and it is also better than the unfermented herbal extract (Comparative Example 3). It was found that the inhibitory effect of the production of prostaglandins according to Formulation Examples 1 to 3 was high.

Claims (6)

Cosmetic composition for improving acne skin, characterized by containing bamboo sap. According to claim 1, wherein the composition is a cosmetic composition for improving acne skin, characterized in that the use of antimicrobial propionibacterium acnes causing the acne. According to claim 1, wherein the composition is a cosmetic composition for improving acne skin, characterized in that the use for controlling sebum secretion. According to claim 1, wherein the composition is a cosmetic composition for improving acne skin, characterized in that it is intended to reduce the generation of cotton. According to claim 1, wherein the composition is a cosmetic composition for improving acne skin, characterized in that the use of anti-inflammatory. The composition according to any one of claims 1 to 5, wherein the composition has a formulation of softening cream (skin lotion), nutritious cream (milk lotion), nutrition cream, massage cream, pack, gel, lipstick, makeup base or foundation. Cosmetic composition for improving acne skin, characterized in that.