KR102592984B1 - Cosmetic Composition Comprising High Molecular Weight Sericin - Google Patents

Abstract

The present invention provides a stable oil-in-water emulsified cosmetic composition that does not precipitate or agglomerate even though it contains high molecular weight sericin, which is insoluble in purified water at room temperature, by increasing the solubility of high molecular weight sericin using a polysaccharide polymer.

Description

Cosmetic composition comprising high molecular weight sericin {Cosmetic Composition Comprising High Molecular Weight Sericin}

The present invention relates to a cosmetic composition containing sericin protein. More specifically, the present invention relates to a cosmetic composition stably containing high molecular weight sericin extracted from silkworm cocoons or raw silk silk.

Sericin is one of the light proteins and is also called silk glue. It is a protein that makes up silkworm cocoon fibers along with fibroin. In silkworm cocoon fiber, two strands of fibroin are covered with three layers of sericin. The rough and hard feel of raw silk is due to this sericin attached, and when refined, it melts and disappears, giving it the unique texture of silk thread. In addition, the amino acid composition of sericin is characterized by the ratio of polar amino acids such as Ser, Asp, and Thr accounting for more than 60%. This amino acid composition is similar to the distribution of proteins that make up human skin, and in fact, sericin is known to have various skin benefits such as whitening, anti-inflammatory, and collagen synthesis ability.

Sericin has previously been discarded as a waste liquid during the silk refining process, but recent research is being actively conducted to utilize it as a functional material in the fields of new bio-related materials, cosmetics, and food. There are various methods used to refine sericin, such as alkali refining, high-temperature and high-pressure treatment, and enzyme treatment. In the case of the commonly used alkaline refining method, refining wastewater intended for disposal is used as the material, and in order to recover sericin from this, a separate process is required to filter out the surfactant or base remaining in the refined wastewater, making it economically viable. The efficiency is low, and it is difficult to recover pure sericin. In general, sericin obtained using alkali refining and enzyme treatment methods is hydrolyzed and has a low molecular weight of approximately 15 kDa or less. This low molecular form of sericin is easily soluble in water, so it is used in cosmetics and food fields.

Numerous documents are referenced and cited throughout this specification. The disclosures of the cited documents are incorporated herein by reference in their entirety to more clearly explain the content of the present invention and the level of technical field to which the present invention pertains.

Republic of Korea Patent Publication No. 10-2033073

The purpose of the present invention is to provide an oil-in-water cosmetic composition prepared by dissolving polymer sericin and a method for producing the same.

Another object of the present invention is to provide a cosmetic composition that improves soft and smooth spreadability and improved absorption by stably dissolving the polymer sericin, which has low solubility in water, and a method for producing the same.

Further objects and advantages of the present invention will become clearer from the following detailed description, claims, and drawings.

One aspect of the present invention is to provide a cosmetic composition containing a polysaccharide polymer and polymer sericin.

Sericin is known to have effects such as whitening, anti-inflammatory, and collagen synthesis, and has a high ratio of polar amino acids, so it can have surface activity ability. The hydrophilic parts (Ser, Asp, Arg) of sericin react with the aqueous solution part, and the hydrophobic part (Gly, Tyr, Val) reacts with the oil layer, lowering the interfacial tension. As the molecular weight increases, the hydrophilic and hydrophobic parts become larger, and the amphotericity increases, thereby increasing the ability to adsorb at the interface. This indicates that sericin can be used as a surfactant, and when it adsorbs to the interface to form an emulsion, sericin helps form small particles and increases stability. The known HLB value of sericin is about 18 and can be used as an emulsifier in O/W formulations. In addition to emulsifying ability, in general, when polymer raw materials are dissolved in water and used in formulations, the sense of adhesion and moisturization are often increased, so in the case of sericin, such improvement in feeling of use can also be expected.

However, unlike low-molecular-weight sericin, which dissolves in water at room temperature, high-molecular-weight sericin with an average molecular weight of 25 kDa or more has poor solubility in water, making it difficult to apply to cosmetics.

In particular, when sericin is treated at high temperature and pressure using water as a solvent, an aqueous solution of high molecular weight sericin with an average molecular weight of 50,000 or more can be obtained. In such aqueous solutions, the solubility of sericin in water is low, so the aqueous solution takes the form of a gel suspended at 0 to 40°C. Also, by freeze-drying it, sericin in powder form can be obtained. When the sericin obtained in this way is dissolved in water again, its solubility decreases and it does not dissolve easily. This decrease in solubility causes aggregation and precipitation of sericin and reduces the uniformity of the aqueous solution. Therefore, it can be said that improving solubility is essential in order to utilize polymer sericin in the form of an aqueous solution.

Accordingly, the present inventors used a polysaccharide polymer to significantly increase the solubility of high molecular weight sericin, preferably high molecular weight sericin with a molecular weight of 25 kDa to 500 kDa, thereby providing a cosmetic composition in an oil-in-water emulsion formulation stably containing high molecular sericin. reached. More specifically, by dissolving the polymer sericin through a polysaccharide polymer, a composition with improved soft and smooth spreadability and improved absorption was prepared.

In the composition of the present invention, high molecular weight sericin is a protein with an average molecular weight of 25 kDa or more, with a molecular weight of 50 kDa or more, 100 kDa or more, 150 kDa or more, 200 kDa or more, 250 kDa or more, 300 kDa or more, 350 kDa or more, 400 kDa or more. , 450 kDa or more, or 500 kDa or more.

In the past, polysaccharide polymers have been used in cosmetic formulations to improve moisturization and nutrition or to improve viscosity, but it was not known that polysaccharide polymers could be used to dissolve the polymer sericin, which is insoluble in purified water at room temperature, in purified water.

Xanthan gum, one of the representative polysaccharide polymers, is a water-soluble polymer extracted from glucose or corn syrup. Xanthan gum has excellent heat resistance and acid resistance, and while the viscosity of general thickeners decreases when acid or alkali substances coexist, xanthan gum shows little change in viscosity depending on pH. In addition, polysaccharide polymers such as cellulose gum and alginic acid can be used. In this way, the polysaccharide polymer of the present invention can be very useful in preparing an O/W type emulsion formulation because it can control the viscosity of the formulation at the same time as dissolving the polymer sericin.

Specifically, the polysaccharide polymer used in the present invention includes gum, alginic acid, sodium alginate, calcium alginate, starch, agar carrageenan, cellulose polymer, gellan, pectin, DEXTRAN, glucan, and glucomannan. One or more may be selected from the group consisting of (GLUCOMANNAN), ARABINO GALACTAN, FURCELLERAN, PULLULAN, GLUCOSAMINE, and GELATIN, but are not limited thereto. .

The gums include xanthan gum, dehydroxanthan gum, cellulose gum, Hydrolyzed Cellulose Gum, carrageenan gum, guar gum, Styrax Benzoin Gum, and sclerotium gum. (Sclerotium Gum), Biosaccharide Gum, ARABIC GUM, LOCUST BEAN GUM, Tamarind Gum, GHATTI GUM, Ceratonia Siliqua Gum ), Astragalus Gummifer Gum, Hydrolyzed Rhizobian Gum, Sclerotium Gum, etc., but are not limited thereto.

The cellulose-based polymers include Calcium Carboxymethyl Cellulose, Carboxymethyl Cellulose Acetate Butyrate, Carboxymethyl Hydroxyethylcellulose, Cellulose Acetate, and Cellulose Acetate Butyrate. Acetate Butyrate, Cellulose Acetate Propionate, Cellulose Acetate Propionate Carboxylate, Cellulose Succinate, Cetyl Hydroxyethylcellulose, Ethylcellulose (Ethylcellulose), Hydroxybutyl Methylcellulose, Hydroxyethylcellulose, Hydroxyethyl Ethylcellulose, Hydroxypropylcellulose, Hydroxypropyl Methylcellulose (Hydr oxypropyl Methylcellulose), Methylcellulose, hydroxypropyl methylcellulose acetate/sucynate, methylcellululose, methylcellulose, methylethyl Methyl ethylcellulose, methyl hydroxyethylcellulose, US crystalline cellulose (methyl hydroxyethylcellulose) Microcrystalline Cellulose, Potassium Cellulose Succinate, Sodium Cellulose Sulfate, etc., but are not limited thereto.

When adding this polysaccharide polymer, it was confirmed that the solubility of polymer sericin in water was improved and aggregation and precipitation between polymer sericins were reduced. Additionally, it was observed that the solubility was further improved when a high temperature of 50°C or higher was applied. The reason why polysaccharide polymer improves the solubility of polymer sericin is not clear, but when the hydrophobic group of the polymer adsorbs to the hydrophobic region of polymer sericin, since the hydrophilic group is an ionic electrostatic repulsion force within or between sericin molecules occurs, leading to the unfolding state of sericin ( It is presumed that this is because it promotes random structure and at the same time prevents aggregation between polymer sericin molecules.

The concentration of the polysaccharide polymer required for such dissolution is not particularly limited, but the weight ratio of the polysaccharide polymer to the polymeric sericin contained in the composition is preferably 0.3 to 5:1. Additionally, the content of polysaccharide polymer contained in the entire composition is preferably 0.03 to 1% by weight.

On the other hand, although the solubility of sericin powder may increase with the addition of polyacrylate-based polymers, polyacrylate-based polymers may cause instability such as creaming of the formulation and phase separation due to a decrease in viscosity when used with salt. There is a problem that when used in large quantities, it causes the formulation to become sticky and reduces the feeling of use.

Another aspect of the present invention includes preparing an aqueous phase by dispersing polysaccharide polymer and polymer sericin in purified water; Preparing an oil phase containing oil; And a step of emulsifying by mixing the water phase and the oil phase, wherein the polysaccharide polymer dissolves polymer sericin, which is water-insoluble at room temperature, in purified water. An oil-in-water (O/W) emulsified cosmetic containing polymer sericin. The purpose is to provide a method for manufacturing the composition.

After dispersing polymer sericin in purified water, adding an oil phase containing oil to it and emulsifying it with a homogenizer, an experiment was conducted to observe whether an emulsified emulsion was created. As a result, when an oil phase of the same composition was added to an aqueous phase that did not contain a polysaccharide polymer, it emulsified. No particles were formed and an oil film was separated, but when the oil phase was added to an aqueous solution of polymer sericin containing a polysaccharide polymer and emulsified, emulsified particles were observed and the oil film was not separated and an emulsion was formed. Through this, it was confirmed that the polysaccharide polymer plays a role in dissolving the water-insoluble polymer sericin in purified water at room temperature, making it possible to produce a stable oil-in-water emulsified cosmetic composition without precipitation or aggregation.

The oil component included in the oil-in-water cosmetic composition according to the present invention is used without particular restrictions as long as it is a component that can be commonly used in the art, for example, silicone oil, vegetable oil, animal oil, ester oil, or hydrocarbon oil. etc. can be used. More specifically, hydrocarbon-based oils such as squalane, mineral oil, hydrogenated polydecene, hydrogenated polyisobutene, and C12-15 alkylbenzoate; Caprylic/capric triglyceride, neopentyl glycol dicaprate, 2-octyldodecyl myristate, isopropyl myristate, isopropyl palmitate, isocetyl ethyl hexanoate, pentaerythrityl tetraethyl hexanoate, Ester oils such as butylene glycol dicaprylate/caprate, hexyl laurate, diisostearyl maleate, cetyl 2-ethylhexanoate, octyldodecanol, and glyceryl triethylhexanoate; Vegetable oils such as olive oil, avocado oil, jojoba oil, macadamia seed oil, grape seed oil, and rice bran oil; Animal oils such as lanolin; and silicone oils such as dimethylpolysiloxane, methylphenylpolysiloxane, decamethylcyclopentasiloxane, methylctrimethicone, diphenylsiloxyphenyltrimethicone, caprylylmethicone, phenyltrimethicone, cyclomethicone, and dimethicone. Any one or more oils selected from the group may be used, but are not limited thereto.

In addition to the ingredients described above, the oil phase may include various ingredients commonly used in the oil phase of oil-in-water cosmetic compositions in the art. For example, liquid oils such as silicone oils, polar oils, and non-polar oils, solid oils, and semi-solid oils may be included, but are not limited thereto.

In addition, the cosmetic composition according to the present invention contains, in addition to the above-mentioned ingredients, thickeners, dispersants, powders, moisturizers, powders, alcohols, natural polymers, and synthetic polymers commonly mixed in oil-in-water cosmetics, to the extent that they do not impair the effect of the present invention. , sugars, antioxidants, buffering agents, various extracts, stabilizers, preservatives, colorants, or fragrances, etc. can be mixed in appropriate amounts and produced according to conventional methods.

The present invention also provides a cosmetic product comprising a cosmetic composition containing the polymer sericin as described above. The above cosmetics include nutritional lotion, softening lotion, pack, softening water, emulsion, makeup base, essence, sunscreen cream, sun oil, suspension, emulsion, paste, gel, lotion, powder, oil, emulsion foundation, foundation, solution, It can be manufactured in formulations such as external ointment, cream, foam, patch, and spray, but is not limited thereto.

The present invention provides a cosmetic composition prepared by dissolving polymer sericin and improving feel of use, such as spreadability. According to the present invention, when the solubility of high molecular sericin in water was improved by using a polysaccharide polymer and then an oil-in-water (O/W) type emulsion was prepared, it was confirmed that the feeling of use on the skin, such as spreadability, was improved. This shows that sericin, which is discarded as refining wastewater, can be applied to the cosmetics field as an emulsifying ability and improver for feeling of use. Sericin is a protein derived from natural products that is skin-friendly because it has an amino acid composition similar to that of skin, and can contribute to not only surfactant ability but also moisturizing effect and high adhesion in cosmetic formulations.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.

Example

In order to solve the problems of the prior art and confirm the function of the present invention, experiments and measurements were conducted at each step as follows.

1. Extraction of high molecular weight sericin through high temperature and pressure treatment

To extract high-molecular sericin, put silkworm cocoons or raw silk washed 1-3 times with lukewarm water in a high-temperature, high-pressure device (autoclave), add 10-100 times the amount of water compared to the weight of the cocoon or raw silk, close the lid, seal, and store at 100℃~. It was treated at 150°C for 15 to 90 minutes. Preferably, the extract was recovered after treating silkworm cocoons or raw silk at high temperature and pressure for 20 to 60 minutes at 100 to 120°C at a bath ratio of 1:15 to 1:20. The recovered extract was filtered through a sieve of 100-200 mesh size, cooled with a freeze-drying device, and treated with gamma rays to obtain polymer sericin in powder form. The molecular weight of the extracted polymer sericin was confirmed through SDS-PAGE, and the average molecular weight was confirmed to be approximately 80 kDa.

2. Comparison of solubility in water of high molecular weight sericin and low molecular weight sericin

To observe the solubility of extracted sericin in water, sericin was dissolved in purified water at a concentration of 0.1% as shown in Table 1 below, heated, and the degree of precipitation was visually confirmed.

In order to compare with the extracted polymer sericin, an aqueous solution of standard sericin was prepared by dissolving Sericin Bombyx mori (molecular weight ~15 kDa), commercially available from Sigma Aldrich, in water at the same concentration.

room temperature 50 ℃ 80 ℃ Extracted polymer sericin 0.1%
insoluble
insoluble
some dissolved Standard Sericin 0.1%
Dissolution
Dissolution
Dissolution

As shown in Table 1 above, it was confirmed that polymer sericin was insoluble in water (partially soluble) even at high temperatures, and 15 kDa sericin was observed to be soluble in water even at room temperature. Therefore, it was confirmed that the solubility of high molecular weight sericin in water was significantly lower than that of low molecular weight sericin. In addition, to investigate the surface activity ability of polymer sericin, polymer (Sodium polyacrylate) and Carbomer were dispersed in purified water, and sericin was dissolved by adding 0, 0.5, and 1.0%, respectively, and then the oil phase was added to prepare an emulsion. As a result of the experiment, it was confirmed that the oil layer separated immediately after emulsification in the formulation without sericin, and when 0.5% and 1% of sericin was added, emulsified particles were observed to be formed. Therefore, it was confirmed that the polymer sericin prepared above had surface activity ability.

3. small molecule Containing sericin and polymeric sericin respectively cosmetics of composition feeling of use comparison

A cosmetic composition was prepared according to the composition in Table 2 below.

Control group:
Sericin-free Addition of low molecular weight sericin polymer
Sericin
adding GLYCERYL STEARATE 0.5 0.5 0.5 PEG-100 STEARATE 0.5 0.5 0.5 HYDROGENATED POLYISOBUTENE 3 3 3 MACADAMIA TERNIFOLIA SEED OIL 4 4 4 NEOPENTYL GLYCOL DICAPRATE 2 2 2 DIMETHICONE 0.9 0.9 0.9 CYCLOPENTASILOXANE One One One Purified water To 100 To 100 To 100 DIPROPYLENE GLYCOL 6 6 6 1,2-HEXANEDIOL 2 2 2 TRISODIUM EDTA Appropriate amount Appropriate amount Appropriate amount TROMETHAMINE Appropriate amount Appropriate amount Appropriate amount HYDROGENATED LECITHIN 1.3 1.3 1.3 FATTY ALCOHOL 0.9 0.9 0.9 BEHENIC ACID 0.4 0.4 0.4 standard sericin 0 0.1 0 Extracted polymer sericin 0 0 0.1 SODIUM POLYACRYLATE 0.04 0.04 0.04 CARBOMER 0.22 0.22 0.22

The skin application effects of the control group, which did not contain the above sericin, and the cosmetic compositions containing low-molecular-weight sericin and high-molecular-weight sericin were compared. As low-molecular-weight sericin, standard sericin (Sigma Aldrich, Sericin Bombyx mori) with a molecular weight of ~15 kDa was used, and as high-molecular-weight sericin, the sericin extracted above was used. The evaluation items were three items: fit, moisture, and thickness, and were evaluated and compared on 20 women aged 20 to 30. For each evaluation standard, the better the effect for each item, the higher the score. The average value of the scores given by each panel is shown in Table 3 below.

<Indicator>

2: Very bad

4: Not good

6: Normal

8: Excellent

10: Very good

Here, “adhesion” is evaluated with a value of 2 to 10, where 2 indicates low adhesion when applied to the skin and a large feeling of peeling, and 10 indicates very excellent adhesion when applied to the skin.

“Moistness” is evaluated on a scale of 2 to 10, where 2 indicates a feeling of dryness when applied to the skin, and 10 indicates a feeling of a lot of moisture when applied to the skin.

“Click feeling” is evaluated on a scale of 2 to 10, where 2 indicates that the product is absorbed when applied to the skin and leaves little residual feeling without a feeling of being coated, and 10 indicates that a coating film is formed after being absorbed when applied to the skin, providing a sticky feeling without pulling. .

Evaluation items Control: without sericin Contains low molecule sericin Contains polymeric sericin Provides a sense of adhesion when applied to the skin 7.9 8.0 9.2 Feels moisturized when applied to skin 8.8 8.9 9.3 Forms a filmy feel when applied to the skin 8.2 8.2 8.7

As shown in Table 3, it was confirmed that overall satisfaction with the adhesion, moistness, and thickness of the formulation increased when high-molecular sericin was included compared to the control group that did not contain sericin and the case that contained low-molecular sericin.

4. Observe solubility by dissolving polymeric sericin in various polymer aqueous solutions

In order to dissolve the extracted polymer sericin in water, various types of polymers, including polysaccharide polymers, were added to water and observed to see whether they were solubilized with the naked eye. 0.1% of sericin was dispersed in 2% 1,2-hexanediol aqueous solution, 0.1% of each polymer was added, and the solubility change was observed at room temperature and heating. As polysaccharide polymers, we used used. The results of observing solubility are shown in Table 4 below.

additives room temperature 50℃ 80℃ Water (distilled water) insoluble insoluble some dissolved Xanthan Gum
0.1% some dissolved Mostly dissolved dissolved Alginic Acid
0.1% some dissolved Mostly dissolved dissolved Cellulose Gum
0.1% some dissolved Mostly dissolved dissolved Sodium Polyacrylate
0.1% some dissolved Mostly dissolved dissolved Polyacrylic Acid0.1% some dissolved Mostly dissolved dissolved Ammonium Acryloyldimethyltaurate/VP Copolymer
0.1% some dissolved Mostly dissolved dissolved Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer0.1% some dissolved Mostly dissolved dissolved

As shown in Table 4 above, when no polymer was added, the polymer sericin was not dissolved in water, and when 0.1% of the polysaccharide polymer was used, polyacrylate polymer, Ammonium Acryloyldimethyltaurate/VP Copolymer, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer It was confirmed that the polymer sericin was dissolved at the same level as when added.

5. Comparison of spreadability when adding sericin to polymer aqueous solution

When the polymer sericin was dissolved using a polymer as described above, the friction force of the aqueous solutions was measured and compared to determine the difference in spreadability of each aqueous solution, and the results are shown in Table 5 below. The device used to measure friction in this experiment was the PARAm (FPT-F1) Friction/Peel Tester. After applying 0.03 g of the formulation onto artificial leather using a 200 g weight, it was applied on a stainless steel plate at a speed of 100 ml/min. The experiment was conducted by measuring the friction force while moving the object 100 mm.

Raw material name (% by weight) Xanthan Gum 0.1 0 0 0 0 0 0 Alginic Acid 0 0.1 0 0 0 0 0 Cellulose Gum 0 0 0.1 0 0 0 0 Sodium Polyacrylate 0 0 0 0.1 0 0 0 Polyacrylic Acid 0 0 0 0 0.1 0 0 Ammonium Acryloyldimethyltaurate/VP Copolymer 0 0 0 0 0 0.1 0 Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer 0 0 0 0 0 0 0.1 Sericin 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Friction force N _Aver. 0.636 0.703 0.619 0.732 0.728 0.892 0.895

As a result of the experiment, it was confirmed that when sericin was added to the gum polymer aqueous solution, the average friction force value was lower than that of the polyacrylate polymer and acryloyldimethyl taurate polymer aqueous solution. This indicates that the spreadability can be smoothly improved when sericin is dissolved with a polysaccharide polymer rather than with a polyacrylate-based polymer or acryloyldimethyltaurate-based polymer.

In addition, in order to confirm the desired content ratio of xanthan gum and sericin, xanthan gum was added to a 0.1% sericin aqueous solution at a concentration of 0, 0.03, 0.05, 0.1, 0.2, and 0.5% of the total concentration, and the solubility and friction of each aqueous solution were checked. .

Raw material name (% by weight) Aqueous solution 1 aqueous solution 2 aqueous solution 3 aqueous solution 4 aqueous solution 5 aqueous solution 5 Xanthan Gum 0 0.03 0.05 0.1 0.2 0.5 Sericin 0.1 0.1 0.1 0.1 0.1 0.1 Friction force N _Aver. 1.818 1.059 0.884 0.636 0.418 0.427

As a result of solubility observation, it was confirmed that dissolution occurred when xanthan gum was more than 30% by weight compared to sericin. In addition, as a result of checking the friction force, it was confirmed that when xanthan gum was more than 30% of the weight of sericin, sufficient dissolution of sericin was achieved and spreadability was improved, and the friction value was noticeably reduced. When the weight ratio of xanthan gum and sericin was less than 0.3:1, sericin was not sufficiently dissolved. Therefore, it was confirmed that the preferable content of xanthan gum is more than 30% of the weight of sericin.

In order to determine the desirable upper limit of xanthan gum, 1% and 1.5% aqueous solutions with increased xanthan gum content compared to the above aqueous solutions 1 to 5 were prepared and friction force comparison and sensory evaluation were performed.

Raw material name (% by weight) aqueous solution 5 aqueous solution 6 Aqueous solution 7 Xanthan Gum 0.5 1.0 1.5 Sericin 0.1 0.1 0.1 Friction force NAver. 0.427 0.517 0.666

As a result of comparing friction force, it was confirmed that when the content of xanthan gum was more than 1%, the friction value no longer decreased but rather increased. In addition, a sensory evaluation was conducted upon skin application to determine whether this increase in friction was related to emotional quality. The two evaluation items were stickiness and stiffness, and were evaluated and compared on 20 women aged 20 to 30. For each evaluation standard, the smaller the effect for each item, the higher the score. The average value of the scores given by each panel is shown in Table 8 below.

<Indicator>

2: Very bad

4: Not good

6: Normal

8: Excellent

10: Very good

Here, “stickiness” is evaluated on a scale of 2 to 10, where 2 indicates a strong sticky feeling when applied to the skin, and 10 indicates that it is not sticky when applied to the skin.

“Stickness” is evaluated on a scale of 2 to 10, with 2 indicating a strong feeling of stiffness when applied to the skin, and 10 indicating that it is applied smoothly and not thick when applied to the skin.

Evaluation items aqueous solution 5 aqueous solution 6 Aqueous solution 7 stickiness 8.6 6.3 4.2 Stiff 9.1 6.1 3.7

As a result of the sensory evaluation, it was confirmed that when xanthan gum was more than 1%, that is, when the weight ratio of xanthan gum and sericin was more than 10:1, stickiness and stiffness significantly increased when applied to the skin. Therefore, it can be inferred that using less than 1% of xanthan gum in the formulation will not deteriorate the emotional quality of cosmetics.

6. Comparison of spreadability of cosmetic compositions prepared by adding polymer and sericin together

To compare the spreadability of a formulation prepared by dissolving sericin with a polysaccharide polymer and a formulation prepared by dissolving sericin with other polymers (polyacrylate-based polymer and acryloyldimethyltaurate-based polymer, etc.), the composition is shown in Table 9 below. Each emulsion was prepared and the friction force was measured. The manufacturing method is as follows.

Glycerin, 1,2-Hexanediol, Trisodium EDTA, Tromethamine, and Sericin were added to purified water, heated to 75°C, and the polymer was sufficiently dispersed. Separately, Carbomer, a thickener, was dispersed in purified water. Caprylic/capric triglyceride, dimethicone, cyclopentasiloxane, glyceryl stearate, PEG-100 stearate, and fatty alcohol were added to the oil phase and heated to 75°C to dissolve them. The water phase was heated to 75°C, the oil phase was slowly added, mixed for 3 minutes with a homomixer, and Carbomer was added and mixed for 5 minutes. Manufacturing was completed by cooling it to 28°C.

Raw material name (% by weight) Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 GLYCERYL STEARATE 0.5 0.5 0.5 0.5 0.5 0.5 0.5 PEG-100 STEARATE 0.5 0.5 0.5 0.5 0.5 0.5 0.5 CAPRYLIC/CAPRIC TRIGLYCERIDE 7 7 7 7 7 7 7 DIMETHICONE One One One One One One One CYCLOPENTASILOXANE One One One One One One One Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 GLYCERIN 6 6 6 6 6 6 6 1,2-HEXANEDIOL 2 2 2 2 2 2 2 TRISODIUM EDTA Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount TROMETHAMINE Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount FATTY ALCOHOL 0.5 0.5 0.5 0.5 0.5 0.5 0.5 CARBOMER 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Sericin 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Xanthan Gum 0.1 0 0 0 0 0 0 Alginic Acid 0 0.1 0 0 0 0 0 Cellulose Gum 0 0 0.1 0 0 0 0 Sodium Polyacrylate 0 0 0 0.1 0 0 0 Polyacrylic Acid 0 0 0 0 0.1 0 0 Ammonium Acryloyldimethyltaurate/
VP Copolymer 0 0 0 0 0 0.1 0 Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer 0 0 0 0 0 0 0.1 Friction force N _Aver. 0.314 0.504 0.443 0.652 0.634 0.525 0.660

As a result of the evaluation, as shown in Table 9, when the polymer sericin is contained in the same amount, the formulation containing It was confirmed that the average value of friction force was significantly lower than that of the formulation containing taurate copolymer. Through this, it was confirmed that a formulation prepared by dissolving sericin using a polysaccharide polymer can have a smoother and softer application compared to a formulation prepared by dissolving sericin using a polyacrylate-based polymer and an acryloyldimethyltaurate-based polymer.

7. Sensory evaluation of cosmetic compositions prepared by adding polymer and sericin together

Using the compositions 1 to 7 prepared above, a sensory evaluation was conducted to compare the feeling of use when applied to the skin between a formulation prepared by dissolving sericin with a polysaccharide polymer and a formulation prepared by dissolving sericin with a polyacrylate polymer.

The three evaluation items were applicability, adhesion, and absorption, and were evaluated and compared on 20 women aged 20 to 30. For each evaluation standard, the better the effect for each item, the higher the score. The average value of the scores given by each panel is shown in Table 10 below.

<Indicator>

2: Very bad

4: Not good

6: Normal

8: Excellent

10: Very good

Here, “spreadability” is evaluated on a scale of 2 to 10, where 2 indicates that the product is not smooth and feels thick when applied to the skin, and 10 indicates that it is soft and smooth when applied to the skin.

“Sense of adhesion” is evaluated on a scale of 2 to 10, where 2 indicates low adhesion when applied to the skin and a high feeling of sticking, and 10 indicates excellent adhesion when applied to the skin.

“Absorption power” is evaluated on a scale of 2 to 10, where 2 indicates slow absorption when applied to the skin, and 10 indicates fast absorption when applied to the skin.

As shown in Table 10 above, when polymeric sericin is contained in the same amount, the formulation containing Compared to formulations containing copolymer, it was confirmed that overall satisfaction with the feeling of application, adhesion, and absorption when applied to the skin was high. Through this, it was confirmed that the formulation manufactured by dissolving sericin using a polysaccharide polymer had an improved feeling of use compared to the formulation manufactured by dissolving sericin using a polyacrylate-based polymer and an acryloyldimethyltaurate-based polymer.

The preferred content range of the polysaccharide polymer may include 0.03 to 1 part by weight of polysaccharide relative to the total composition. If it exceeds 1 part by weight, flowability may deteriorate and feel of use may be reduced due to stickiness.

Claims (10)

delete delete delete delete delete delete delete delete Preparing an aqueous phase by dispersing a polysaccharide polymer and high molecular weight sericin with a molecular weight of 25 kDa to 500 kDa in purified water;
Preparing an oil phase containing oil; and
Comprising the step of mixing the water phase and the oil phase to emulsify,
The polysaccharide polymer is a method for producing an oil-in-water (O/W) emulsified cosmetic composition containing high molecular weight sericin, characterized in that high molecular weight sericin, which is water-insoluble at room temperature, is dissolved in the purified water,
The polysaccharide polymers include gum, alginic acid, sodium alginate, calcium alginate, starch, agar carrageenan, cellulose polymer, gellan, pectin, DEXTRAN, glucan, glucomannan, and arabinogalactan. At least one selected from the group consisting of (ARABINO GALACTAN), FURCELLERAN, PULLULAN, GLUCOSAMINE and gelatin,
A method for producing an oil-in-water (O/W) emulsified cosmetic composition in which the high molecular weight sericin and the polysaccharide polymer are dispersed together in an aqueous phase.

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