KR102597685B1 - Oral composition comprising parsley extract - Google Patents

Abstract

The present invention relates to a composition for preventing or treating oral diseases, and more specifically, to an oral composition containing parsley extract as an active ingredient. The oral composition containing the parsley extract of the present invention is excellent in preventing or treating oral diseases, is non-toxic because it is derived from natural products, and does not cause side effects when applied to the human body, making it highly useful.

Description

Oral composition comprising parsley extract}

The present invention relates to an oral composition, and more particularly, to an oral composition for preventing or treating oral diseases.

As the concept of dental health changes to the concept of social life, interest in oral health is increasing, and interest in bad breath, which is an important obstacle to interpersonal relationships in social life as well as prevention and treatment of oral diseases, is also increasing.

Tooth tissue is composed of enamel, cementum, and dentin. The outside of the tooth exposed to the outside is covered with enamel, and the outside of the tooth root located within the alveolar bone is covered with cementum, and dentin exists inside these. Dentin has microscopic tubes called dentinal tubules distributed throughout the dentin.

The symptom of sensitive teeth is that the dentin part of the exposed tooth is felt sensitive when it comes in contact with cold air or irritating food. The fundamental cause of this symptom is that many tubules present in the dentin of the tooth are exposed to the outside. Symptoms of sensitive teeth can range from mild symptoms to intense and persistent pain. In addition, because teeth do not regenerate due to their nature, taking painkillers or anti-inflammatory drugs is not a fundamental solution to the phenomenon of sensitive teeth, so various treatments have been developed to alleviate the symptoms of sensitive teeth.

There are two main treatments for sensitive teeth: dentinal tubule sealing and pulp nerve desensitization. The dentinal tubule sealing method is a method of restoring lost enamel by filling the exposed dentinal tubules with resin or applying fluoride, while the pulp nerve desensitization method regulates nerve transmission by the appropriate ratio of sodium ions and potassium ions. This is a treatment that prevents nerves from feeling pain by artificially increasing the potassium ion content. However, because this specialized treatment requires the hassle of going to the dentist, toothpastes for sensitive teeth that can treat or prevent sensitive teeth in everyday life are being sold.

These toothpastes contain active ingredients useful for the above two treatment methods. However, among these, toothpastes that contain potassium phosphate and other ingredients to show the effect of desensitizing the pulpal nerve have a long period of effect, about 15 days, so toothpastes that have the effect of dentinal tubule sealing, which shows the effect relatively quickly, are preferred. there is.

However, in the case of Sirin Professional toothpaste, which utilizes the conventional dentinal tubule sealing effect, it contains fine powder of the active ingredient to seal the dentinal tubules, but it is usually quickly diluted during the toothbrushing process and most of it is washed away during the rinsing process with water. There was a problem in that the customs tube was not sealed and was exposed again.

Meanwhile, the causes of bad breath are largely classified into intra-oral causes, extra-oral causes, and psychogenic causes. Among them, oral causes account for about 80-90% of the causes of bad breath, resulting in decreased saliva volume, increased saliva consistency, and hydrogen ion concentration buffering capacity. decrease, increase in the amount and activity of oral microorganisms, tongue coating attached to the back of the tongue, and oral diseases such as untreated dental caries and periodontal disease.

Meanwhile, among oral diseases, dental caries and periodontal disease are known to cause various clinical symptoms such as pain, impaired chewing function, destruction of periodontal tissue, and bad breath, and are the main factors causing tooth loss. Changes in eating habits are the cause of dental caries. The number of factors is increasing.

It is a method of suppressing the growth of oral pathogens that live in the dental plaque and cause dental caries, periodontal disease, and bad breath. Various types of antibacterial agents, including antibiotics with sterilizing and bacteriostatic effects on these bacteria, are used to treat dental caries, periodontal disease, dental pulp, and other diseases. It has been developed as a treatment and inhibitor of periapical infection.

However, antibiotics have the disadvantage of being difficult to use long-term and being used only as a treatment because they can cause systemic side effects on our body and the emergence of resistant bacteria in the oral cavity and bacterial turnover. In addition, fluoride, an antibacterial agent, has a clear cavity prevention effect, but its effect on periodontal, dental pulp, or periapical diseases has not yet been reported. In addition, antibacterial agents used in toothpaste, mouthwash, and other mouthwashes include sanguinarine, listerine, peroxide, and chlorhexidine. Sanguinarine is effective against bacteria in the oral cavity. Listerine's main ingredient is alcohol, so it has a temporary effect or a slight bacteriostatic effect in the oral cavity, but it may also have a harmful effect on tissues when used for a long period of time. Peroxide, which has recently been added for whitening effect, has a toxic effect on bacteria, but at the same time, it has a toxic effect on human tissue, so not only is there a safety problem, but sometimes bacteria resistant to peroxide emerge. do. In addition, chlorhexidine is known to be the best among the agents known to date as a preventive and treatment agent for periodontal disease in addition to inhibiting plaque formation. However, it has the side effects of tissue irritation, tissue staining and degeneration, and especially a strong and irritating taste. In addition, there are disadvantages that it cannot be used for long periods of time for treatment or especially prevention purposes, such as it can cause bacterial dysbiosis, and its use in pregnant women is limited due to its carcinogenic properties (Contemporary Periodontics [ed. R.J. Genco et. al.], p161-169, p368-369, p433, C.V. Mosby Company, St. Louis, 1990).

Accordingly, the present inventors conducted research to derive a natural material that is effective in preventing and improving oral diseases such as dental caries, periodontal disease, bad breath, and sensitive teeth without concerns about the above-mentioned side effects.

Republic of Korea Patent Publication No. 10-2004-0051902

Accordingly, the present inventors completed the present invention by confirming that parsley extract has excellent effects on oral diseases.

Therefore, an object of the present invention is to provide an oral composition containing parsley extract as an active ingredient.

Additionally, an object of the present invention is to provide an oral product containing the composition.

As an embodiment to solve the above problem, the present invention provides an oral composition containing parsley ( Petroselinum crispum ) extract as an active ingredient.

In the present invention, the composition may be used to prevent or treat oral diseases.

In the present invention, the oral disease is a comprehensive concept that includes, but is not limited to, bad breath, sensitive teeth, dental caries, gingivitis, periodontitis, oral mucosal ulcer, and periapical disease.

In the present invention, the oral disease may preferably be syrin.

As used herein, the term “prevention” refers to all actions that suppress or delay disease using the oral composition. Additionally, the term “treatment” used herein refers to any action in which symptoms of a disease are alleviated or completely cured using the oral composition.

In addition, the term "alleviation" used herein means reducing or alleviating the symptoms of oral diseases such as those described above, and can be understood as a broad concept including improvement, prevention, treatment, etc.

Additionally, the term “improvement” used in this specification can be understood as a broad concept meaning to restore symptoms to close to their original state by treating oral diseases, etc.

The term "bad breath" used in this specification is not limited to this, but causes bad odor due to the decomposition of substances produced by microorganisms in the oral cavity due to acquired systemic diseases or when proteins and food residues in saliva are decomposed. It can occur by producing substances that The oral microorganisms that are the primary cause of bad breath are, but are not limited to, Streptococcus genus, Porphyromonas genus, Actinomyces genus, Actinobacillus genus, etc. This can be.

As used herein, the term “dental caries” is not limited thereto, but means that teeth are damaged by bacteria in the oral cavity, resulting in cavities. For example, plaque, a biofilm formed on the surface of teeth, may be the cause. The oral bacteria may be of the Streptococcus genus, Porphyromonas genus, etc.

The terms “gingivitis” and “periodontitis” used in this specification are, but are not limited to, conditions for the growth of bacteria such as Streptococcus and Porphyromonas present in subgingival plaque. When it forms, it can cause infection in the soft tissue of the gingival area and develop into periodontal disease such as gingivitis or periodontitis, which is an oral disease that causes inflammation of the gums.

The term "oral mucosal ulcer" used in this specification is also called stomatitis, but is not limited to this, and is not limited to oral mucosa (skin, gums, lips, inner cheek) due to infection by oral bacteria, viruses, fungi, etc. etc.) refers to a disease in which inflammation occurs, and the bacteria may be bacteria of the Streptococcus genus or Porphyromonas genus.

The term “periapical disease” used herein is not limited thereto, but may mean inflammation occurring at the root end of the tooth. Periapical diseases include periapical cysts, periapical abscesses, and periapical osteosarcomas, but are not limited to these and can be caused by bacteria such as Streptococcus and Porphyromonas .

The term "sirini" used herein is not limited thereto, but refers to any phenomenon in which sharp, temporary or persistent pain appears in response to external stimuli, independently of cavities or other pathological causes. The above-mentioned external stimulus usually refers to temperature stimulus, and symptoms are usually reported in response to cold temperature stimulation, but pain may also appear in hot temperature. In addition to temperature stimulation, pain can also occur due to stimulation such as drying of the teeth, contact with external substances, or osmotic pressure from sweet or sour foods. This may appear throughout the entire tooth, or may be limited to specific areas such as the upper or lower jaw, or the right or left, and may be accompanied by dentine hyperesthesia, caries, and pulp inflammation.

Exposure to the entrance of the dentinal tubules can be considered the cause of these symptoms.

When enamel or cementum is destroyed and the entrance of the dentinal tubule is exposed to the outside, stimulation is transmitted to the pulp by the cell protrusions, nerves, and dentinal fluid of the dentinal tubule, causing pain.

The inventors of the present invention completed the present invention by confirming that parsley extract has a property that allows it to be easily absorbed into the tooth surface and/or dentinal tubules, and thus has an excellent effect in preventing and alleviating the symptoms of sensitive teeth.

As used herein, the term "extract" refers to an extract obtained by extraction treatment of parsley, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a crude product or purified product of the extract, or a mixture thereof, etc. This includes the extract itself and all formulations of the extract that can be formed using the extract.

The parsley extract of the present invention can be extracted from various organs of the natural, hybrid, or variant plant of parsley, for example, parsley seeds, roots, aerial parts, stems, leaves, flowers, and fruit body, It can be extracted not only from the fruit peel but also from plant tissue culture.

The parsley extract of the present invention may preferably be an extract of parsley seeds or aerial parts, and more preferably an extract of the aerial parts. In the present invention, the above-ground part is not limited to any above-ground part of parsley, but may preferably be the entire above-ground part.

In the parsley extract of the present invention, the method of extracting the parsley is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include solvent extraction, hot water extraction, ultrasonic extraction, filtration, and reflux extraction, which may be performed alone or in combination of two or more methods, preferably using a solvent. This can be performed using an extraction method.

The type of extraction solvent used to extract the parsley in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, One or more solvents selected from the group consisting of ethyl ether and cyclohexane may be used. When alcohol is used as the solvent, C ₁ to C ₄ alcohols (methanol, ethanol, propanol, butanol) are preferably used. there is. In the present invention, water or distilled water can be more preferably used as an extraction solvent for the parsley.

The oral composition of the present invention is Streptococcus genus or Porphyromonas genus, more specifically Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguis. sanguinis ), Streptococcus salivarius spp. Thermophils ( Streptococcus salivarius) subsp. thermophils ), and Porphyromonas gingivalis It was confirmed that it has an excellent antibacterial effect on one or more bacteria selected from the group consisting of (Gingivalis ) and is effective in preventing and treating oral diseases.

More preferably, the oral composition of the present invention may have an excellent antibacterial effect against Streptococcus mutans or Porphyromonas gingivalis bacteria.

In the present invention, the parsley extract may be 1 to 10% by weight based on the total weight of the composition, preferably 3 to 7% by weight based on the total weight of the composition, and more preferably 4% by weight compared to the total weight of the composition. It may be from 6% by weight. If the parsley extract is less than 1% by weight of the total weight of the composition, the effect on oral diseases may be minimal, and if it is more than 10% by weight, it is undesirable because the feeling of use, such as taste when brushing teeth, is not good.

In addition to the parsley extract, the oral composition of the present invention may further include ingredients such as wetting agents, abrasives, medicinal agents, sweeteners, pH adjusters, preservatives, binders, flavoring agents, and foaming agents, depending on the formulation and purpose of use. .

Wetting agents are used alone or in combination from the group consisting of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycol, and propylene glycol, and the amount used may be 1 to 70% by weight based on the total weight of the oral composition. .

The abrasive is one selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, ground calcium carbonate, calcium pyrophosphate, insoluble metaphosphate, and aluminum silicate. etc. can be used. The amount of such abrasive used may generally be 1 to 60% by weight based on the total weight of the oral composition. Additionally, additives used in small amounts may include commonly used ingredients such as sweeteners, pH adjusters, preservatives, colorants, and binders.

Medicinal agents include sodium fluoride, sodium monofluorophosphate, sodium fluoride, stannous fluoride, chlorhexidine, allantoin chlorohydroxyaluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc., singly or in combination. The above can be mixed and used.

As binders, sodium carboxymethyl cellulose, carbomer, carrageenan, xanthan gum, alginates, etc. can be used, and as foaming agents, anionic and nonionic surfactants such as sodium alkyl sulfate, sodium lauryl sulfate, sucrose fatty acid ester, and sorbitan fatty acid ester can be used. It can be used alone or in combination of two or more types. The amount of this binder used is generally 0.1 to 3% by weight, preferably 0.5 to 2% by weight, based on the total weight of the oral composition.

Sweeteners include saccharin, xylitol, erythritol, aspartame, etc., and the amount of such sweeteners used may generally be 0.05 to 2% by weight based on the total weight of the oral composition.

As pH adjusters, sodium phosphate, disodium phosphate, citric acid, triethanolamine, etc. can be used.

Preservatives include benzoic acid, methylparaben, propylparaben, and sodium benzoate. As a fragrance, a mixture of peppermint oil, spearmint oil, and menthol may be used, and enzymes such as dextranase may be used as other additives.

Foaming agents include sodium lauryl sulfate, an anionic surfactant, copolymers of polyoxyethylene and polyoxypropylene (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, etc., which are nonionic surfactants. . The amount of such foaming agent used is generally 0.5 to 5% by weight, preferably 0.5 to 3.5% by weight, based on the total weight of the oral composition.

Additionally, it contains a remaining amount of water.

According to another embodiment of the present invention, an oral product containing the composition is provided.

The oral composition according to the present invention, which contains parsley extract as an active ingredient, is an oral composition that can be included in all products generally manufactured and sold for oral health, and there is no limitation to the oral products to which it is applied. For example, the oral products include, but are not limited to, toothpaste, oral spray, mouthwash, oral ointment, mouthwash, gargling product, gum, etc. The oral product of the present invention may be in the form of liquid, solid, suspension, gel, or aerosol, but is not limited thereto.

The oral composition containing the parsley extract of the present invention is excellent in preventing or treating oral diseases.

In addition, the composition of the present invention is non-toxic because it is derived from natural products, and does not cause side effects when applied to the human body, making it highly usable.

Hereinafter, to aid understanding of the present invention, it will be described in detail through examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Ingredients Preparation

Preparation of parsley extract

After pulverizing about 100 g of the aerial part of parsley, 200 mL of distilled water was added, extracted once at room temperature, and then filtered to obtain a water extract.

< Experiment example 1>

Sirin Comparison of phenomenon suppression levels

In order to compare the degree of suppression of toothpaste, sodium carboxymethyl cellulose, sodium lauryl sulfate, glycerin (concentrated glycerin (98%)), and sorbitol aqueous solution (70%), which can be commonly used to manufacture toothpaste, are shown in Table 1 below. A control toothpaste was made using silica (precipitated silica), xanthan gum, sodium fluoride, flavoring, saccharin, citric acid, and triethanol amine (TEA), and an experimental group toothpaste containing 5% by weight of parsley extract. was manufactured.

Ingredient name (unit: weight%) Experimental group (Example) Control group (comparative example) precipitated silica 20.0 20.0 Concentrated glycerin (98%) 30.0 30.0 Sorbitol solution (70%) 30.0 30.0 Sodium lauryl sulfate 1.0 1.0 Sodium Carboxymethylcellulose 0.5 0.5 xanthan gum 0.5 0.5 parsley extract 5 - sodium fluoride 0.22 0.22 perfume 1.0 1.0 saccharin 1.0 1.0 Citric acid, Triethanol amine (TEA) Appropriate amount Appropriate amount Purified water To 100 To 100

The degree of inhibition of the cold noise phenomenon of the compositions prepared above was compared and measured. In other words, the subjects of the study were 40 volunteers with hyperesthesia teeth who agreed to participate in this experiment, and the total number of teeth subject to the study was 80. Additionally, among the applicants, there were 20 men and 20 women, and their ages ranged from 20 to 50 years old. The study subjects were blinded to the contents of the toothpaste, and the total test period was 2 weeks.

The test was conducted by measuring the patient's response after applying a temperature stimulus. Before conducting the test, the hypersensitivity area of each applicant's hypersensitive teeth was checked in advance, and cold water at about 5°C was dropped with a dropper on the sensitive area of the tooth to give a rating, and each composition was used three times a day for two weeks. After 2 weeks, cold water at about 5°C was added again using a dropper and the score was evaluated. The patient's response rating criteria after stimulation were set as follows. (0 points: no discomfort, 1 point: slightly uncomfortable or sore, 3 points: painful). For statistical processing, the stimulation scores before and 2 weeks after the laboratory test were tested using a paired student-t test.

The response scores to temperature stimulation after 2 weeks are shown in Table 2 below.

control group experimental group 0 weeks 2.23±0.21 2.22±0.16 2 weeks 2.22±0.05 1.12±0.23 p>0.05 *p<0.05

< Experiment example 2>

Bad breath removal effect experiment

To compare the degree of bad breath removal, control and experimental group toothpastes were prepared with the same composition as Table 1 above.

Fifty men and women without dental caries were selected and a cross-over test was conducted on the target drinks. Commercially available garlic powder was dispersed in water and left for 24 hours, then diluted to obtain a Halimeter measurement value of 700 ppb or higher, and the diluted solution was used as a bad breath inducer. After gargling with 15ml of garlic powder dilution for 30 seconds, the level of bad breath was measured with a halimeter 1 minute later, and then brushing the teeth using control and experimental group toothpaste for 30 seconds to 1 minute. After 1 minute, 5 minutes, and 30 minutes of brushing teeth, the degree of bad breath was measured with a halimeter to determine whether the suppression of bad breath lasted. The results are shown in Table 3 below.

control group experimental group 1 min 60.4% 96.1% 5 minutes 41.7% 83.7% 30 minutes 31.9% 76.9%

< Experiment example 3>

Antibacterial effect experiment

A paper disk test was used to determine the effectiveness of inhibiting strains using the gram-positive bacterium Streptococcus mutans , which is a representative cause of dental caries and periodontal disease, and the gram-negative bacterium Porphyromonas gingivalis . An antibacterial test was conducted. Each of the above bacteria was activated under the optimal culture conditions shown in Table 4 below, and then cultured in the optimal medium for each bacteria for about 4 to 6 hours to adjust the turbidity of the culture medium to Macfarland turbidity No. After adjusting to 0.5 (1.5x10 ⁸ ), 0.1 ml was evenly spread. Parsley extract was inoculated at a concentration of 10 mg/disc on a sterilized paper disk (Whatman no. 5 paper, 8 mm diameter), absorbed and dried for 1 hour, and then used. After culturing for 24-48 hours at the optimal temperature for each of the above bacteria, the size (diameter mm) of growth-inhibiting rings was measured.

strain optimal conditions Gram staining Strain name temperature badge characteristics Gram (+) Streptococcus mutans 37℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37℃ TSA Hemin Menadione medium Anaerobic

The results are as shown in Table 5 below.

Growth retardation ring diameter (mm) S. mutans P. gingivalis Untreated - (No inhibition) - (No inhibition) Parsley Extract Treatment 9.9 15.5

As shown above, the composition according to the present invention was confirmed to have an excellent effect on oral diseases such as bad breath, sensitive teeth, dental caries, gingivitis, periodontitis, oral mucosal ulcers, and periapical diseases.

Claims (14)

An oral composition for preventing or treating syringitis, comprising parsley extract as an active ingredient. According to paragraph 1,
The parsley extract is prepared by mixing parsley with water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, An oral composition for the prevention or treatment of syphilis, characterized in that it is a solvent extract extracted by adding at least one solvent selected from the group consisting of ethyl ether and cyclohexane. According to paragraph 1,
An oral composition for preventing or treating syringitis, wherein the parsley extract is a water or distilled water extract. According to paragraph 1,
An oral composition for the prevention or treatment of syringitis, characterized in that the parsley extract is obtained by extracting the seeds or aerial parts of chives. According to paragraph 1,
An oral composition for the prevention or treatment of syphilis, characterized in that the parsley extract is 1 to 10% by weight based on the total weight of the composition. According to paragraph 1,
The parsley extract is an oral composition for preventing or treating sensitive teeth, characterized in that the parsley extract is adsorbed on the tooth surface or dentinal tubules. An oral product for the prevention or treatment of syringitis, comprising the oral composition of any one of claims 1 to 6. An antibacterial oral composition against Streptococcus mutans or Porphyromonas gingivalis , comprising parsley extract as an active ingredient. According to clause 8,
The parsley extract is prepared by mixing parsley with water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, An oral composition for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, characterized in that it is a solvent extract extracted by adding at least one solvent selected from the group consisting of ethyl ether and cyclohexane. According to clause 8,
An oral composition for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, wherein the parsley extract is a water or distilled water extract. According to clause 8,
An oral composition for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, wherein the parsley extract is obtained by extracting the seeds or aerial parts of chives. According to clause 8,
An oral composition for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, wherein the parsley extract is 1 to 10% by weight based on the total weight of the composition. According to clause 8,
The parsley extract is an oral composition for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, wherein the parsley extract is adsorbed on the tooth surface or dentinal tubules. An oral product for antibacterial use against Streptococcus mutans or Porphyromonas gingivalis, comprising the oral composition of any one of claims 8 to 13.