KR102509579B1 - Oral composition comprising cassava extract - Google Patents

Abstract

The present invention relates to a composition for preventing or treating oral diseases, and more particularly, to a composition for oral cavity containing a cassava extract as an active ingredient. The oral composition containing the cassava extract of the present invention is excellent in preventing or treating oral diseases, is non-toxic because it is a material derived from natural products, and has high utilization because it does not cause side effects even when applied to the human body.

Description

Oral composition comprising cassava extract {Oral composition comprising cassava extract}

The present invention relates to a composition for oral cavity, and more particularly, to a composition for oral cavity for preventing or treating oral diseases.

As the concept of dental health changes to the concept of social life, interest in oral health is increasing, and interest in bad breath, which is an important interpersonal obstacle in social life as well as prevention and treatment of oral diseases, is also increasing.

Tooth tissue is composed of enamel, cementum and dentin. The outside of the tooth exposed to the outside is covered with enamel, the outside of the tooth root portion located in the alveolar bone is covered with cementum, and dentin exists inside them. In dentin, fine tubes called dentinal tubules are distributed throughout the dentin.

Tooth ache symptoms are sensitive when the exposed dentin part of the tooth comes into contact with cold air or irritating food. Toothache symptoms can range from mild symptoms to intense and persistent pain. In addition, since teeth do not regenerate by nature, taking painkillers or anti-inflammatory drugs is not a fundamental solution to the ache, so various treatments have been developed to alleviate the symptoms of ache.

There are two main treatments for syringitis: dentinal tubule sealing and pulpal nerve desensitization. Dentinal tubule sealing method is a method to restore enamel lost by filling exposed dentinal tubules with resin or applying fluoride, etc., and pulp nerve desensitization method is that neurotransmission is controlled by an appropriate ratio of sodium ions and potassium ions. It is a treatment that prevents the nerves from feeling the pain by artificially increasing the content of potassium ions in consideration of However, these professional treatment procedures are cumbersome to go to the dentist, so toothpastes for cold teeth that can treat or prevent cold teeth in everyday life are sold.

These dentifrices contain active ingredients useful for the above two treatment methods. However, among these, toothpaste containing dibasic potassium phosphate component to exhibit pulpal nerve desensitization effect takes as long as 15 days, so toothpaste with efficacy by dentinal tubule sealing method, which shows efficacy relatively quickly, is preferred. there is.

However, in the case of a toothpaste specializing in toothpaste that utilizes the conventional dentinal tubule sealing effect, it contains fine powder of active ingredients to seal the dentinal tubules. There was a problem that the customs could not be sealed and exposed again.

On the other hand, the causes of halitosis are largely classified into oral, extraoral, and psychogenic causes. Among them, oral causes account for 80 to 90% of the causes of bad breath. decrease in the amount and activity of oral microbes and oral diseases such as tongue plaque attached to the dorsal surface of the tongue and untreated dental caries and periodontal disease.

On the other hand, among oral diseases, dental caries and periodontal disease cause various clinical symptoms such as pain, masticatory dysfunction, destruction of periodontal tissue and bad breath, and are known to be the main factors that cause tooth loss, and changes in dietary life are the cause of dental caries. elements are on the rise.

It is a method of inhibiting the growth of oral pathogens that live in dental bacterial film and cause dental caries, periodontal disease, and bad breath. It has been developed as an inhibitor and treatment agent for apical infection.

However, since antibiotics can cause the appearance of resistant bacteria in the oral cavity and mycosis, along with systemic side effects on our body, long-term use is difficult and can only be used as a therapeutic agent. In addition, in the case of fluoride, which is an antibacterial agent, the effect of preventing caries is clear, but no effect on periodontal, dental pulp, or apical diseases has been reported yet. In addition, antibacterial agents used in toothpaste, mouthwash, and other mouthwashes include sanguinarine, listerine, peroxide, and chlorhexidine. Sanguinarine is effective against bacteria in the oral cavity. Listerine has the disadvantage of being unclear and the effect of treating periodontal disease is not only unclear, but also expensive. Listerine has alcohol as its main component and has a temporary effect or a slight bacteriostatic effect in the oral cavity. Peroxide, which has recently been added for whitening effect, has a toxic effect on bacteria, but at the same time it also exhibits a toxic effect on human tissues, causing safety problems and sometimes bacteria resistant to peroxide may appear in bacteria. do. In addition, chlorhexidine is known to be the best among the agents known to date as a preventive and therapeutic agent for periodontal disease as well as to inhibit plaque formation. In addition, there are disadvantages in that it cannot be used for a long period of time for the purpose of treatment or prevention, such as that it can cause mycosis and is carcinogenic, limiting its use in pregnant women (Contemporary periodontics [ed. R.J. Genco et al. al.], p161-169, p368-369, p433, C.V. Mosby Company, St. Louis, 1990).

Accordingly, the present inventors have conducted research to derive natural materials that are effective in preventing and improving oral diseases, such as dental caries, periodontal diseases, halitosis, and ache, without worrying about the side effects as described above.

Republic of Korea Patent Publication No. 10-2014-0031512

Accordingly, the present inventors completed the present invention by confirming that the cassava extract has an excellent effect on oral diseases.

Accordingly, an object of the present invention is to provide a composition for oral use comprising a cassava extract as an active ingredient.

Another object of the present invention is to provide an oral product comprising the composition.

As one embodiment for solving the above problems, the present invention provides an oral composition comprising a cassava ( Manihot esculenta ) extract as an active ingredient.

In the present invention, the composition may be used for preventing or treating oral diseases.

In the present invention, the oral disease is a comprehensive concept including, but not limited to, bad breath, tooth decay, dental caries, gingivitis, periodontitis, mucosal ulcers in the oral cavity, and apical diseases.

In the present invention, the oral disease may preferably be syringi.

As used herein, the term "prevention" refers to any activity that suppresses or delays a disease by using the composition for oral cavity. In addition, as used herein, the term "treatment" refers to all activities in which the symptoms of a disease are alleviated or cured by using the composition for oral cavity.

In addition, as used herein, the term "relieving" means reducing or mitigating the oral disease symptoms, etc., and can be understood as a broad concept including improvement, prevention, treatment, and the like.

In addition, the term "improvement" used herein may be understood as a broad concept meaning restoring symptoms to an original state by treating oral diseases and the like.

The term "halitosis" as used herein is not limited thereto, but is caused by acquired systemic diseases or by decomposition of proteins in saliva, food waste, etc. can occur by creating a substance that Oral microorganisms that are the primary cause of bad breath include, but are not limited to, Streptococcus genus, Porphyromonas genus, Actinomyces genus, Actinobacillus genus, etc. This can be.

As used herein, the term "dental caries" means, but is not limited to, dental caries caused by damage to teeth by bacteria in the oral cavity. For example, plaque, which is a biofilm formed on the surface of a tooth, may be the cause. The bacteria in the oral cavity may be Streptococcus genus, Porphyromonas genus, and the like.

As used herein, the terms "gingivitis" and "periodontitis" are, but are not limited to, bacteria present in subgingival plaque ( Streptococcus ), Porphyromonas ( Porphyromonas ), and the like are suitable growth conditions When formed, it may be an oral disease that infects the soft tissues of the gingival area, develops into periodontal disease of gingivitis or periodontitis, and causes inflammation of the gums.

As used herein, the term "oral mucosal ulcer" is also referred to as stomatitis, but is not limited thereto, due to infection by bacteria, viruses, fungi, etc. Etc.) means an inflammatory disease, and the bacteria may be bacteria such as the genus Streptococcus and the genus Porphyromonas .

As used herein, the term "apical disease" may mean, but is not limited to, inflammation of the apical end of the tooth root. Apical diseases include apical cyst, apical abscess, apical osteosarcoma, etc., but are not limited thereto, and may be caused by bacteria such as Streptococcus genus and Porphyromonas genus.

As used herein, the term "cold ear" refers to any phenomenon in which sharp, temporary or continuous pain is manifested in response to external stimuli, apart from tooth decay or other pathological causes, but is not limited thereto. The external stimulus usually means a temperature stimulus, and usually complains of symptoms in a cold temperature stimulus, but pain may appear in a hot temperature. In addition to these temperature stimuli, pain may also be caused by stimuli such as tooth drying, contact with external substances, and osmotic pressure through sweet or sour food. It may appear throughout the entire tooth, may be limited to a specific area such as the upper or lower jaw, or the right or left side, or may be accompanied by dentine hyperesthesia, caries, or pulp inflammation.

Exposure of the entrance of the dentinal tubules can be considered as the cause of these symptoms.

When the enamel or cementum is destroyed and the entrance of the dentinal tubule is exposed to the outside, stimulation is transmitted to the dental pulp by the cell protrusions, nerves, and dentin fluid of the dentinal tubule, so that pain can be felt.

The inventors of the present invention have completed the present invention by confirming that the cassava extract has a property that can be well adsorbed on the tooth surface and/or within the dentinal tubules, so that it has an excellent effect on preventing and alleviating the symptoms of syringitis.

As used herein, the term "extract" refers to an extract obtained by extraction of cassava, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, It includes the extract itself and extracts of all formulations that can be formed using the extract.

The cassava extract of the present invention can be extracted from various organs of natural, hybrid or mutant plants of cassava, for example, seeds (seeds), roots, aerial parts, stems, leaves, flowers, fruit bodies of cassava, It can be extracted not only from the skin of the fruit but also from plant tissue culture.

The cassava extract of the present invention may preferably be an extract of seeds or aerial parts of cassava, more preferably an extract of aerial parts. In the present invention, the above-ground portion is not limited to any part of the above-ground portion of cassava, but may preferably be the entire above-ground portion.

In the cassava extract of the present invention, the method for extracting the cassava is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include solvent extraction, hot water extraction, ultrasonic extraction, filtration, reflux extraction, and the like, which may be performed alone or in combination of two or more methods, preferably solvent extraction. This can be done using an extraction method.

In the present invention, the type of extraction solvent used to extract the cassava is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, di and one or more solvents selected from the group consisting of ethyl ether and cyclohexane, and the like. When alcohol is used as the solvent, preferably, C ₁ to C ₄ alcohols (methanol, ethanol, propanol, butanol) can be used. there is. In the present invention, more preferably, water or distilled water can be used as the cassava extraction solvent.

The oral composition of the present invention is Streptococcus genus or Porphyromonas genus ( Porphyromonas ) genus, more specifically, Streptococcus mutans ( streptococcus mutans ), Streptococcus sanguis ( streptococcus sanguis ), Streptococcus sanguinis ( Streptococcus sanguinis ), Streptococcus salivarius species Thermophils ( Streptococcus salivarius subsp. thermophils ), and Porphyromonas gingivalis ( Porphyromonas gingivalis ), it was confirmed that the antibacterial effect was excellent on one or more bacteria selected from the group consisting of, and thus, there was an excellent effect in preventing and treating oral diseases.

More preferably, the oral composition of the present invention may have an excellent antibacterial effect against Streptococcus mutans or Porphyromonas gingivalis bacteria.

In the present invention, the cassava extract may be 1 to 10% by weight based on the total weight of the composition, preferably 3 to 7% by weight based on the total weight of the composition, more preferably 4% by weight based on the total weight of the composition to 6% by weight. If the cassava extract is less than 1% by weight relative to the total weight of the composition, the effect on oral diseases may be insufficient, and if it is more than 10% by weight, it is not preferable because the feeling of use such as taste is not good when brushing teeth.

In addition to the cassava extract, the oral composition of the present invention may further include a wetting agent, an abrasive, a medicinal agent, a sweetening agent, a pH adjusting agent, a preservative, a binder, a flavoring agent, a foaming agent, etc. .

The humectant is used alone or in combination in the group consisting of concentrated glycerin, glycerin, sorbitol aqueous solution, amorphous sorbitol aqueous solution, polyethylene glycols and propylene glycol, and the amount used may be 1 to 70% by weight relative to the total weight of the oral composition. .

The abrasive is one selected from the group consisting of precipitated silica, silica gel, zirconium silicate, calcium monohydrogen phosphate, anhydrous calcium monohydrogen phosphate, hydrous alumina, light calcium carbonate, heavy calcium carbonate, calcium pyrophosphate, insoluble metaphosphate and aluminum silicate. etc. can be used. The amount of the abrasive used may generally be 1 to 60% by weight based on the total weight of the oral composition. In addition, additives used in small amounts may include sweeteners, pH adjusters, preservatives, colorants, and binders as commonly used components.

Sodium fluoride, sodium monofluorophosphate, sodium fluoride, tin fluoride, chlorhexidine, allantoin chlorohydroxyaluminate, aminocaproic acid, zinc chloride, pyridoxine hydrochloride, tocopherol acetate, enzymes, etc. alone or in combination It can be used by mixing more than one.

Sodium carboxymethylcellulose, carbomer, carrageenan, xanthan gum, alginate, etc. can be used as a binder, and anionic and nonionic surfactants such as sodium alkyl sulfate, sodium lauryl sulfate, sucrose fatty acid ester, sorbitan fatty acid ester, etc. can be used as a foaming agent. It can be used individually or in mixture of 2 or more types. The amount of the binder used is generally 0.1 to 3% by weight, preferably 0.5 to 2% by weight, based on the total weight of the oral composition.

Saccharin, xylitol, erythritol, aspartame, etc. may be used as the sweetener, and the amount of the sweetener used may be 0.05 to 2% by weight based on the total weight of the oral composition.

As the pH adjusting agent, sodium phosphate, disodium phosphate, citric acid, triethanolamine, and the like can be used.

Benzoic acid, methyl paraben, propyl paraben, sodium benzoate, etc. may be used as preservatives. Peppermint oil, spearmint oil, menthol, etc. may be mixed and used as flavoring agents, and enzymes such as dextranase may be used as other additives.

As the foaming agent, sodium lauryl sulfate as an anionic surfactant, polyoxyethylene polyoxypropylene copolymer (poloxamer) as a nonionic surfactant, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, etc. may be used. . The amount of these foaming agents is generally 0.5 to 5% by weight, preferably 0.5 to 3.5% by weight, based on the total weight of the oral composition.

In addition, the residual amount of water is included.

According to another embodiment of the present invention, an oral product comprising the composition is provided.

The oral composition according to the present invention containing cassava extract as an active ingredient is a composition for oral use that can be included in all commercially available products generally manufactured for oral health, and the applied oral products are not limited. For example, the oral product includes, but is not limited to, toothpaste, oral spray, mouthwash, oral ointment, mouthwash, gargling product, chewing gum, and the like. The oral product of the present invention may be in the form of a liquid, solid, suspension, gel, or aerosol, but is not limited thereto.

Oral composition containing the cassava extract of the present invention is excellent in preventing or treating oral diseases.

In addition, the composition of the present invention is not toxic because it is a material derived from natural products, and it does not cause side effects even when applied to the human body, so its utilization is high.

Hereinafter, examples and the like will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention can be modified in many different forms, and the scope of the present invention should not be construed as being limited to the following examples. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

material preparation

Manufacture of Cassava Extract

After crushing about 100 g of the aerial parts of cassava, 200 mL of distilled water was added to extract once at room temperature, followed by filtration to obtain a water extract.

< Experimental example 1>

sirin Comparison of degree of suppression

In order to compare the degree of inhibition of syrin, first, sodium carboxymethylcellulose, sodium lauryl sulfate, glycerin (concentrated glycerin (98%)), sorbitol aqueous solution (70%), which can be generally used as shown in Table 1 below, to prepare toothpaste , Silicas (precipitated silica), xanthan gum, sodium fluoride, fragrance, saccharin, citric acid, triethanolamine (TEA), etc. were used to make a control toothpaste, and an experimental group toothpaste containing 5% by weight of cassava extract was manufactured.

Ingredient name (unit: % by weight) Experimental group (Example) Control group (comparative example) precipitated silica 20.0 20.0 Agriglycerin (98%) 30.0 30.0 Sorbitol aqueous solution (70%) 30.0 30.0 sodium lauryl sulfate 1.0 1.0 Sodium Carboxymethyl Cellulose 0.5 0.5 xanthan gum 0.5 0.5 Cassava Extract 5 - sodium fluoride 0.22 0.22 spices 1.0 1.0 saccharin 1.0 1.0 Citric acid, Triethanol amine (TEA) Appropriate amount Appropriate amount Purified water To 100 To 100

The degree of suppression of shirin of the compositions prepared above was compared and measured. That is, 40 volunteers who agreed to participate in this experiment as people with hypersensitive teeth were studied, and the total number of teeth for the study was 80. In addition, 20 men and 20 women were among the volunteers, and their ages ranged from 20 to 50 years. The subjects of the study were unaware of the contents of the toothpaste, and the total test period was 2 weeks.

The test was conducted by measuring the patient's response after applying the temperature stimulus. Before the test, the hypersensitive area of each volunteer's hypersensitive teeth was checked in advance, and cold water of about 5 ° C was dropped on the cold part of the tooth with a dropper, and then each composition was used three times a day for 2 weeks. After 2 weeks, cold water at about 5 ° C. was dropped again with a dropper and evaluated. The patient's response rating criteria after stimulation were set as follows. (0 points: no discomfort, 1 point: slightly uncomfortable or cold, 3 points: painful). For statistical processing, stimulation scores before and 2 weeks after the laboratory test were tested by paired student-t test.

The response scores for the temperature stimulation after 2 weeks are shown in Table 2 below.

control group experimental group 0 weeks 2.21±0.71 2.22±0.21 2 weeks 2.21±0.05 1.39±0.15 p>0.05 *p<0.05

< Experimental example 2>

Bad breath removal effect experiment

In order to compare the degree of bad breath removal, toothpastes for the control group and the experimental group were prepared with the configuration shown in Table 1 above.

Fifty men and women without dental caries were selected and a cross-over test was conducted on the target beverage. Commercially available garlic powder was dispersed in water and allowed to stand for 24 hours, then diluted so that the Halimeter measurement value was 700 ppb or more, and the diluted solution was used as a source of bad breath. After gargling with 15 ml of diluted garlic powder for 30 seconds, after 1 minute, the degree of bad breath was measured with a halimeter, and then brushed teeth for 30 seconds to 1 minute using toothpaste from the control group and the experimental group. After 1 minute, 5 minutes, and 30 minutes of brushing the teeth, the degree of bad breath was measured with a halimeter to determine whether or not the bad breath suppression continued. The results are shown in Table 3 below.

control group experimental group 1 min 67.1% 94.2% 5 minutes 46.0% 86.5% 30 minutes 33.7% 74.1%

< Experimental example 3>

Antibacterial effect test

By using the gram-positive bacterium Streptococcus mutans and the gram-negative bacterium Porphyromonas gingivalis , which are representative bacteria that cause dental caries and periodontal disease, the paper disk test method is used to determine whether there is an effect of inhibiting the strain. An antimicrobial activity test was conducted. After cultivating each of the bacteria under the optimal culture conditions in Table 4 below, the turbidity of the culture solution was determined by culturing in the optimal medium for each bacteria for about 4 to 6 hours. After adjusting to be 0.5 (1.5x10 ⁸ ), 0.1 ml was spread evenly. A sterilized paper disk (Whatman no.5 paper, 8 mm diameter) was inoculated with cassava extract at a concentration of 10 mg/disc, and dried by absorption for 1 hour before use. After culturing for 24-48 hours at the optimal temperature of each of the above bacteria, the size (diameter mm) of the growth arrest ring was measured.

strain optimum condition Gram staining strain name temperature badge characteristics Gram (+) Streptococcus mutans 37℃ BHI Facultative anaerobic Gram (-) Porphyromonas gingivalis 37℃ TSA Hemin Menadione medium Anaerobic

The results are as shown in Table 5 below.

Growth arrest ring diameter (mm) S. mutans P. gingivalis untreated - (No inhibition) - (No inhibition) Cassava Extract Treatment 10.2 13.5

As described above, it was confirmed that the composition according to the present invention has an excellent effect on oral diseases such as halitosis, toothache, dental caries, gingivitis, periodontitis, oral mucosal ulcers and apical diseases.

Claims (14)

Contains cassava extract as an active ingredient,
The cassava extract is obtained by extracting the aerial part of cassava,
An oral composition for preventing or treating one or more oral diseases selected from the group consisting of halitosis, ache, and dental caries. delete delete delete According to claim 1,
The cassava extract is obtained by adding water, distilled water, methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, distilled water to cassava. An oral composition characterized in that it is a solvent extract extracted by adding at least one solvent selected from the group consisting of ethyl ether and cyclohexane. According to claim 1,
The oral composition, characterized in that the cassava extract is a water or distilled water extract. delete According to claim 1,
The cassava extract is an oral composition, characterized in that 1 to 10% by weight relative to the total weight of the composition. According to claim 1,
The composition is an oral composition, characterized in that there is an antibacterial effect in the genus Streptococcus or Porphyromonas . According to claim 9,
The Streptococcus genus or Porphyromonas genus includes Streptococcus mutans , Streptococcus sanguis , Streptococcus sanguinis , Streptococcus salivarius species Thermophils ( Streptococcus salivarius subsp. thermophils ), and Porphyromonas gingivalis ( Porphyromonas gingivalis ) Oral composition, characterized in that at least one selected from the group consisting of. According to claim 9,
The Streptococcus ( streptococcus ) or Porphyromonas ( Porphyromonas ) genus Streptococcus mutans ( streptococcus mutans ) or Porphyromonas gingivalis ( Porphyromonas gingivalis ) Oral composition, characterized in that. According to claim 1,
Oral composition, characterized in that the cassava extract is adsorbed on the tooth surface or in the dentinal tubules. An oral product comprising the oral composition of any one of claims 1, 5, 6, and 8 to 12. According to claim 13,
The oral product, characterized in that selected from the group consisting of toothpaste, gargling products, chewing gum, oral spray, oral ointment, mouthwash and mouthwash.