KR102319701B1 - Cosmetic composition comprising mixture of fermented extract of Cirsium japonicum and Moringa oleifera - Google Patents

Abstract

The present invention relates to a fermented extract of hot spring water of milk thistle and moringa, and more particularly, to a cosmetic and food composition for whitening comprising the fermented extract as an active ingredient. The fermented extract of milk thistle and Moringa hot spring water according to the present invention has low cytotoxicity, excellent antioxidant effect, and significant tyrosinase inhibitory activity. can be utilized.

Description

Cosmetic composition comprising mixture of fermented extract of Cirsium japonicum and Moringa oleifera

The present invention relates to a fermented extract of hot spring water of milk thistle and moringa, and more particularly, to a cosmetic and food composition for whitening comprising the fermented extract as an active ingredient.

Fermentation, in its original scientific meaning, refers to a phenomenon in which alcohol, organic acid, carbon dioxide, etc. are produced by breaking carbon bonds when microorganisms use carbohydrates as energy sources. Fermented cosmetics were developed with the focus on the substances produced during the fermentation process. In other words, the function of enzymes in fermented foods we eat in our daily life to activate cells was introduced into cosmetics. Fermentation in cosmetics removes fermenting bacteria in the process of fermenting microorganisms and at the same time extracts active ingredients in addition to enzymes, thereby doubling the efficacy of the existing enzyme alone and allowing the ingredients to be absorbed quickly into the skin.

In general, the interval between skin cells is 70 to 80 nm in size, and in order for cosmetic ingredients to permeate into the skin, the molecular weight of the ingredients must be smaller than the size of the skin cells. To solve this problem, the cosmetics industry has focused on the development of nano-particles or liposome-type microcarriers. However, this method requires a high level of technology and has limitations in that the manufacturing cost is high.

On the other hand, it has been reported that fermented cosmetics can increase the skin absorption rate only through the fermentation process without such advanced technology. In addition, in the process of fermenting raw materials, microorganisms and yeast act to break down molecular particles to 80 nm or less, so it has the advantage of being able to absorb various fermented ingredients to the dermis layer.

On the other hand, Moringa ( Moringa oleifera ) is a perennial plant distributed in tropical and subtropical climate regions, and 14 species are known. Recently, according to the characteristics of each part of Moringa in various countries around the world, it is used in various ways, such as pharmaceuticals, cosmetics, water purification agents, animal feed, and crop growth promoters. Moringa leaves, seeds, flowers, tree trunks, fruits (pods) and roots are all used as food ingredients. Moringa leaves contain 20 to 25% of dry matter compared to other plant leaves. It was reported that the water content was low and the content of various nutrients was relatively high. Moringa leaves are high in protein, as well as minerals such as beta-carotene, vitamin C, carotene, calcium, potassium, and iron, so they are useful enough to be used as dietary supplements to cope with malnutrition in Africa, where food is absolutely scarce, or as nutritious food for mothers. it is a plant

In addition, thistle ( Cirsium japonicum var. ussuriense ) is a perennial herb belonging to the Asteraceae family (Compositae), and 13 species, 6 varieties, and 1 variety are wild in Korea. In oriental medicine, the above-ground part or the underground part (root) is used for medicinal purposes. That is, the above-ground parts such as thistle leaves, stems, flowers and seeds are collected from May to June when the seeds are ripe from the flowering period, and the roots are collected in the fall and dried. , hepatitis and hypertension. Thistle ( Cirsium ) plants are rich in flavonoid-type compounds with excellent physiological activity and have anti-wrinkle, anti-cancer, anti-mutagenic, anti-fungal, neuroprotective and immune-enhancing activities. In addition, milk thistle is known to protect the liver by inhibiting lipid peroxidation and increasing the activity of glutathione reductase to promote alcohol detoxification.

Accordingly, the present inventors have completed the present invention by developing a fermented extract of milk thistle and Moringa hot spring water in order to increase the skin absorption rate of the active ingredient.

Accordingly, it is an object of the present invention to provide a whitening composition comprising milk thistle and fermented Moringa hot spring water extract as active ingredients.

In order to achieve the above object, the present invention provides a cosmetic composition for whitening comprising milk thistle and fermented Moringa hot spring water extract as active ingredients.

In addition, the present invention provides a food composition for whitening comprising milk thistle and fermented moringa hot spring water extract as active ingredients.

The fermented extract of milk thistle and Moringa hot spring water according to the present invention has low cytotoxicity, excellent antioxidant effect, and significant tyrosinase inhibitory effect. can be utilized.

1 is a view showing a method for producing a fermented extract of hot spring water of milk thistle and moringa according to the present invention.
2 is a diagram showing the results of measuring the ABTS radical scavenging ability of the fermented extract of hot spring water of milk thistle and moringa according to the present invention.
3 is a diagram showing the results of measuring the tyrosinase inhibitory activity of the fermented extract of hot spring water of milk thistle and moringa according to the present invention.
4 is a diagram showing the results of measuring the cytotoxicity of the fermented extract of hot spring water of milk thistle and Moringa according to the present invention in Raw264.7 cells, which are macrophages.

Hereinafter, the present invention will be described in detail.

According to an aspect of the present invention, there is provided a cosmetic composition for whitening comprising a fermented extract of hot spring water of milk thistle and moringa as an active ingredient.

In the present invention, the term "extract" refers to a preparation obtained by squeezing a crude drug into an appropriate leaching solution and evaporating the leachate to concentrate the extract. It may be a crude product or a purified product.

The milk thistle and moringa can be used without limitation, such as cultivated or commercially available ones, and can be washed and used as it is or dried. have.

According to a preferred embodiment of the present invention, the thistle is a dried stem, leaf or root.

According to another preferred embodiment of the present invention, Moringa is dried Moringa leaves.

In the present invention, the term “hot spring water” means groundwater whose water temperature is higher than the average annual temperature of the region or the groundwater temperature of the shallow stratum, and in Korea, the temperature is 25° C. or higher, and it means groundwater that is not harmful to the human body.

In an embodiment of the present invention, the hot spring water is preferably Onyang hot spring water obtained in the Asan region, and more preferably, the Onyang hot spring water is potassium (K) 0.05-3 mg/l, sodium (Na) 20-80 mg/l, Calcium (Ca) 2-10 mg/l, silicon (Si) 10-30 mg/l, lithium (Li) 0.01-5 mg/l, strontium (Sr) 0.01-5 mg/l and magnesium (Mg) 0.01- 5 mg/l, and more preferably, the hot spring water contains potassium (K) 1.59 mg/l, sodium (Na) 45.5 mg/l, calcium (Ca) 6.72 mg/l, silicon (Si) 25.85 mg/l l, lithium (Li) 0.12 mg/l, strontium (Sr) 0.27 mg/l, and magnesium (Mg) 0.21 mg/l.

In the present invention, "fermentation" refers to a phenomenon in which alcohol, organic acid, carbon dioxide gas, etc. are produced by breaking the carbon bond of carbohydrate when a microorganism uses carbohydrate as an energy source. Examples of the microorganism is Lactobacillus ramno suspension (Lactobacillus rhamnosus), Lactobacillus para Kasei (Lactobacillus paracasei), Lactobacillus ahrijo norbornene sheath (Lactobacillus arizonensis), a celebrity busy (Saccharomyces cerevisiae), beer yeast (Saccharomyces ellipsoides), Bacillus subtilis Tili ( Bacillus subtilis ), Bacillus licheniformis ( Bacillus licheniformis ) and leukonostoc mesenteroides ( Leuconostoc mesenteroides ) and the like.

In an embodiment of the present invention, the microorganism for fermentation is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) and Lactobacillus paracasei ( Lactobacillus paracasei ) It was used by mixing, but is not limited thereto.

As a suitable solvent for obtaining the fermented extract from the fermented product, water or an organic solvent may be used, and any pharmaceutically acceptable organic solvent may be used. For example, at least one selected from water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof may be used, and water is preferably used.

The extraction temperature is preferably 50 to 150 ℃. In addition, as the extraction method, various methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, compression method, etc. may be used, but are not limited thereto, preferably hot water extraction method or reflux method Cold extraction method is used.

The fermented extract of milk thistle and Moringa hot spring water can be cooled, heated and filtered at room temperature by a conventional method known in the art to obtain a liquid, or the solvent may be further evaporated, spray-dried, or freeze-dried. In addition, the fermented extract of milk thistle and Moringa hot spring water may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze-drying or spray-drying. In addition, the extract is obtained as a fraction further purified using various chromatography methods such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. can

In an embodiment of the present invention, hot water was added to each of milk thistle and moringa for fermentation, and the fermented product was extracted with hot water to obtain each fermented extract of hot water. The fermented extract of thistle hot spring water and the fermented Moringa hot spring water extract were mixed to prepare a fermented extract of milk thistle and Moringa hot spring water.

In an embodiment of the present invention, the fermented extract of thistle and Moringa hot spring water is preferably mixed with a fermented extract of thistle and Moringa hot spring water in a weight ratio of 0.5 to 2:1.

The fermented extract of milk thistle and Moringa hot spring water is preferably contained in an amount of 5 to 1000 µg/ml, more preferably 500 to 1000 µg/ml, with respect to the cosmetic composition. When the content is less than 5 μg/ml, the whitening effect may be insignificant, and when it exceeds 1000 μg/ml, the increase rate of the effect compared to the amount used is low, which may be uneconomical.

The cosmetic composition of the present invention may further include conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, and the like, which are commonly used in cosmetic compositions in addition to the active ingredients. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, and allantoin. .

Since the cosmetic composition of the present invention is basically applied to the skin, it may be prepared in any formulation conventionally prepared with reference to a cosmetic composition in the art. For example, it may be formulated as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray, etc. The present invention is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be included as carrier components. have.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included as carrier components, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane and dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, emulsifier, etc. may be included as carrier components, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like may be included.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol, propylene glycol; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tracanth, and the like may be included.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like may be included.

According to another aspect of the present invention, the present invention provides a food composition for whitening comprising a fermented extract of hot spring water of milk thistle and moringa as an active ingredient.

The fermented extract of milk thistle and moringa hot spring water of the present invention may be used as a health functional food, food additive or dietary supplement.

When the fermented extract of milk thistle and Moringa hot spring water is used as a food additive, the mixture may be added as it is, or it may be appropriately used according to a conventional method, such as being mixed with other foods or food ingredients.

In addition, the mixing amount of the milk thistle and Moringa hot spring water fermented extract may be suitably changed depending on the purpose of use (prevention, health or therapeutic treatment), of course, it is included in 0.01 to 95% by weight based on the total weight of the food composition Preferably, it is included in an amount of 1 to 80% by weight more preferably. If the content is less than 0.01% by weight, the whitening effect may be insignificant, and if it exceeds 95% by weight, the increase rate of the effect relative to the amount used is low, which may be uneconomical.

As a specific example, in the production of food or beverage, the fermented extract of milk thistle and Moringa hot spring water of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw material. However, in the case of long-term intake for health and hygiene or health control, it may be added in an amount less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range. have.

There is no particular limitation on the type of food, but examples of the food to which the fermented extract of milk thistle and Moringa hot spring water of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, There are gums, dairy products including ice cream, various soups, beverages, teas, drinks, alcoholic beverages, vitamin complexes, and the like, and includes all health foods in a normal sense.

When the food composition of the present invention is prepared as a beverage, it may contain additional ingredients such as various flavoring agents or natural carbohydrates like conventional beverages. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; natural sweeteners such as dextrin and cyclodextrin; Synthetic sweeteners such as saccharin and aspartame may be used. The natural carbohydrate is included in an amount of 0.01 to 10% by weight, preferably 0.01 to 0.1% by weight, based on the total weight of the food composition of the present invention.

The food composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may include a carbonation agent used in beverages, and the like, and may include, but is not limited to, the flesh for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. Although the additive ratio is not particularly limited, it is preferably included in the range of 0.01 to 0.1% by weight based on the total weight of the food composition of the present invention.

In the case of long-term ingestion for health and hygiene purposes or for health control, the food composition of the present invention can be taken for a long period of time because there is no problem in terms of safety.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1. Preparation of fermented extract of milk thistle and Moringa hot spring water using hot spring water and lactic acid bacteria

A mixture of fermented extracts of hot spring water of milk thistle and Moringa was prepared in the same manner as in FIG. 1 .

For the thistle used for extraction, the stems, leaves, and roots were finely crushed to 1 to 3 cm, and then crushed to 1,300 mesh again. The crushed milk thistle was sterilized at 120° C. for 20 minutes using an autoclave. Sterilized milk thistle and hot spring water were mixed and homogenized at a ratio of 1:1 (v/v). The Onyang hot spring water was provided by Asan Jeil Tourist Hotel located in Asan, potassium (K) 1.59 mg/l, sodium (Na) 45.5 mg/l, calcium (Ca) 6.72 mg/l, silicon (Si) 25.85 mg/l l, lithium (Li) 0.12 mg/l, strontium (Sr) 0.27 mg/l and magnesium (Mg) 0.21 mg/l. The milk thistle and hot spring water mixture was inoculated with 10% of activated Lactobacillus rhamnosus and Lactobacillus paracasei strains and then fermented for 72 hours at 37° C., 120 rpm and anaerobic conditions. After adding distilled water 10 times the weight of the sample to an extraction flask equipped with a vertical reflux condenser, the fermented product was extracted three times at 100° C. for 60 minutes to obtain a fermented extract of thistle hot spring water.

The fermented Moringa hot spring water extract was prepared using dried Moringa leaves, and was prepared in the same manner as the fermented milk thistle hot spring water extract.

Milk thistle and Moringa hot spring water fermented extract was prepared by mixing the prepared fermented milk thistle hot spring water ferment extract and Moringa hot spring water fermented extract at a ratio of 1:1 (w/w).

In Examples to be described later, fermented extracts of milk thistle and Moringa purified water prepared using purified water instead of hot spring water were used as a control.

Example 2. Measurement of ABTS radical scavenging activity of fermented extracts of milk thistle and Moringa hot spring water

The ABTS cation decolorization assay is a method for measuring antioxidant activity using ABTS radicals. Specifically, 7 mM 2,2-azino-bis(3-ethyl-benthiazoline-6-sμlfonic acid) and 2.45 mM potassium persulfate were mixed and reacted at room temperature for 24 hours to generate ^{ABTS + .} . After that, 100 μl of each sample was added to ^{ABTS +} 100 μl diluted with ethanol, and absorbance was measured at 700 nm. The ABTS scavenging ability was calculated as in Equation 1 below, and the results are shown in FIG. 2 .

[Equation 1]

As shown in FIG. 2 , the ABTS radical scavenging ability increased as the concentration increased in the milk thistle and Moringa hot spring water ferment extract treatment group, and it was confirmed that it was 86.8% in the 500 μg/ml treatment group (milk thistle and Moringa purified water ferment extract) 71.3%). The above result means that the antioxidant ability of the milk thistle and Moringa hot spring water ferment extract treatment group is excellent.

Example 3. Tyrosinase inhibitory activity of fermented extracts of milk thistle and Moringa hot spring water

To measure the tyrosinase inhibitory activity, the reaction group was 200 U/ml mushroom tyrosinase (Sigma) in a mixture of 40 μl of substrate solution and 40 μl of sample solution in which 10 mM L-DOPA (Sigma, USA) was dissolved in 80 μl of 67 mM phosphate buffer (pH 6.8). , USA) 40 μl was added and reacted at 37° C. for 10 minutes. DOPA chromium produced in the reaction solution was measured at 492 nm. The tyrosinase inhibitory activity was calculated as in Equation 2, and the results are shown in FIG.

[Equation 2]

As shown in Figure 3, each experimental group increased the tyrosinase inhibitory activity as the concentration increased. In particular, it was confirmed that the tyrosinase inhibitory activity was 68.5% in the 1,000 μg/ml treatment group of fermented extracts of milk thistle and Moringa hot spring water using hot spring water as a fermentation solvent.

Example 4. Measurement of Cytotoxicity of Milk Thistle and Moringa Hot Spring Water Fermented Extracts

4-1. Cell Lines and Cell Cultures

The macrophage Raw 264.7 used in this Example was cultured using DMEM medium supplemented with 10% FBS and 1% penicillin/streptomycin (penicillin/streptomycin, 100U/ml). Culture conditions were incubated in an incubator at a temperature of 37° C. and 5% CO _{2 .}

4-2. MTT analysis

MTT analysis was conducted according to Carmichael's method. In a 96-well plate, ^{180 μl of Raw264.7, 1X10 5} cells/well, was dispensed. After adding 20 μl of fermented extract of milk thistle and Moringa hot spring water to the seeded cells, _{it was cultured at 37° C., 5% CO 2 in an} incubator for 24 hours. Here, 40 μl of MTT solution prepared at a concentration of 2.5 mg/ml was added and incubated for 4 hours. After incubation, the culture medium was removed, and 100 μl of DMSO was added to each well, reacted for 10 minutes at room temperature, and absorbance was measured at 540 nm with an ELISA reader. The cytotoxicity measurement was expressed as a decrease in absorbance in the group with and without the addition of milk thistle and Moringa hot spring water ferment extract, and was calculated as shown in Equation 3 below. The results of MTT analysis of Raw264.7 on fermented extracts of milk thistle and Moringa hot spring water are shown in FIG. 4 .

[Equation 3]

As shown in FIG. 4 , it was confirmed that the cell viability of Raw264.7 cells treated with milk thistle and Moringa hot spring water ferment extract at a concentration of 5 to 500 μg/ml was greater than 90%.

Hereinafter, the present invention will be described in more detail through formulation examples. The formulation examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by the formulation examples.

Formulation Example 1. Preparation of a cosmetic formulation

1-1. Manufacture of soft lotion

Milk thistle and Moringa hot spring water ferment extract 0.1% by weight, 1,3-butylene glycol 5.2% by weight, oleyl alcohol 1.5% by weight, ethanol 3.2% by weight, polysorbate 20 3.2% by weight, benzophenone-9 2.0% by weight, A softening lotion was prepared by a conventional method by mixing 1.0% by weight of carboxyl vinyl polymer, 3.5% by weight of glycerin, a trace amount of fragrance, a trace amount of a preservative, and the remaining amount of purified water.

1-2. Milk lotion manufacturing

Milk thistle and Moringa hot spring water ferment extract 0.1% by weight, glycerin 5.1% by weight, propylene glycol 4.2% by weight, tocopheryl acetate 3.0% by weight, liquid paraffin 4.6% by weight, triethanolamine 1.0% by weight, squalane 3.1% by weight, macadamia nut oil 2.5 Weight %, polysorbate 60 1.6% by weight, sorbitan sesquirolate 1.6% by weight, propylparaben 0.6% by weight, carboxyvinyl polymer 1.5% by weight, trace amount of fragrance, trace amount of preservative, and the remaining amount of purified water are mixed with conventional Milk lotion was prepared by this method.

1-3. Nutrient cream manufacturing

Milk thistle and Moringa hot spring water ferment extract 0.5% by weight, glycerin 4.0% by weight, petrolatum 3.5% by weight, triethanolamine 2.1% by weight, liquid paraffin 5.3% by weight, squalane 3.0% by weight, beeswax 2.6% by weight, tocopheryl acetate 5.4% by weight, 3.2 wt% of polysorbate 60, 1.0 wt% of carboxyl vinyl polymer, 3.1 wt% of sorbitan sesquioleate, a trace amount of fragrance, a trace amount of preservative and the remaining amount of purified water were mixed to prepare a nourishing cream in a conventional manner.

1-4. massage cream manufacturing

Milk thistle and Moringa hot spring water ferment extract 0.5% by weight, glycerin 4.0% by weight, petrolatum 3.5% by weight, triethanolamine 0.5% by weight, liquid paraffin 24.0% by weight, squalane 3.0% by weight, beeswax 2.1% by weight, tocopheryl acetate 0.1% by weight, A massage cream was prepared in a conventional manner by mixing 2.4 wt% of polysorbate 60, 1.0 wt% of carboxyl vinyl polymer, 2.3 wt% of sorbitan sesquioleate, a trace amount of fragrance, a trace amount of preservative and the remaining amount of purified water.

1-5. Manufacture of body cleanser for cleaning

1g of fermented extract of milk thistle and Moringa hot spring water, 18g of anionic surfactant, 5g of nonionic surfactant, 7g of glycerin, 3g of sodium chloride, 1.5g of natural olive liquid soap, 1g of fragrance and 100g of water are mixed in the usual way for cleansing body cleanser was prepared.

Above, specific parts of the present invention have been described in detail, for those of ordinary skill in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. something to do. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (7)

Contains fermented extracts of thistle hot spring water and moringa hot spring water fermented extracts as active ingredients,
The hot spring water fermented extract is extracted after fermenting by adding hot spring water to milk thistle or moringa,
The milk thistle hot spring water fermented extract and Moringa hot spring water fermented extract are mixed in a weight ratio of 0.5 to 2: 1,
A cosmetic composition for whitening. According to claim 1,
The whitening cosmetic composition, wherein the milk thistle is one or more parts selected from the group consisting of stems, leaves and roots. According to claim 1,
The moringa is a moringa leaf, a cosmetic composition for whitening. delete According to claim 1,
The extract is extracted with one or more solvents selected from water, an alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, a cosmetic composition for whitening. According to claim 1,
The spring water is potassium (K) 0.05-3 mg/l, sodium (Na) 20-80 mg/l, calcium (Ca) 2-10 mg/l, silicon (Si) 10-30 mg/l, lithium (Li) ) 0.01-5 mg/l, strontium (Sr) 0.01-5 mg/l and magnesium (Mg) 0.01-5 mg/l, the cosmetic composition for whitening. delete

Images