KR101532003B1 - Cosmetics comprising fermented materials for skin antioxidant - Google Patents

Abstract

The present invention relates to a cosmetic product including fermented compositions and, more specifically, a cosmetic product for anti-oxidizing skin including fermented compositions made by fermenting leaf of Tilia taquetii C. K. Schneid., lotus, Eucommia ulmoides OLIV. and Paeonia lactiflora, and extracts of Portulaca oleracea L., herb and red ginseng wherein the cosmetic product comprises fermented compositions made by fermenting leaf of Tilia taquetii C. K. Schneid., lotus, Eucommia ulmoides OLIV. and Paeonia lactiflora twice with use of at least one strain selected from a group consisting of aspergillus, aspergillus oryzae, Aspergillus kawachii, Nattobacillus, Bacillus, lactic acid bacteria, yeast and actinomycetales.

Description

COSMETICS COMPRISING FERMENTED MATERIALS FOR SKIN ANTIOXIDANT FIELD OF THE INVENTION [0001]

More particularly, the present invention relates to a skin antioxidant functional cosmetic comprising a fermentation composition obtained by fermenting mulberry leaves, mulberry leaves, peonies and peonies, an extract of Matsuchi, a herb extract and a red ginseng extract.

The cosmetic according to the present invention is characterized in that the mulberry leaves, the long leaves, the bugs, and the peony pests are classified into Aspergillus (Rhizopus), Aspergillus oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast, and the like. And a fermentation composition prepared by primary fermentation and secondary fermentation with at least one strain selected from the group consisting of actinomycetes (actinomycetes).

Cosmetics refers to articles used by the human body to clean or groom the human body and to maintain and promote the health of the skin and hair.

Currently, cosmetics with various functionalities are being developed and released, and consumer interest in functional cosmetics is steadily increasing.

Particularly, research and development on antioxidant functional cosmetics for skin have been actively carried out, and active oxygen is one of the factors affecting skin damage.

The active oxygen generated by various physical, chemical and environmental factors such as in vivo enzymatic system, reductive metabolism, chemical agents, pollutants and photochemical reaction is not selective for the cell components such as lipids, proteins, sugars and DNA, It is known to cause various diseases including cancer and senescence by destructive action.

In addition, various peroxides such as lipid peroxides produced by these active oxygen also cause oxidative destruction of cells and cause various dysfunctions, thereby causing various diseases.

On the other hand, ingredients of cosmetics can be roughly divided into nine kinds such as water, oily raw materials, surfactants, coloring matters, efficacy raw materials, flavoring agents, preservatives, thickeners or moisturizers.

Water usually corresponds to the "purified water" of cosmetic ingredients, which accounts for more than 10% of the product.

Oily ingredients control the loss of moisture in the skin and improve absorption. Representative components include oils, waxes, higher fatty acids, higher alcohols, hydrocarbons, esters, and silicones.

Surfactant is a substance which adsorbs on the interface between two materials and changes its properties. It is composed of emulsifier which makes water and oil mixed well, solubilizer which makes small amount of oil soluble in water, dispersant which uniformly disperses solid particles in water, An external wetting agent, a foaming agent, a defoaming agent, and a detergent.

Moisturizing agent is a water-absorbing material that absorbs dry and keratinized skin and smoothes and softens the skin. Representative examples are glycerin, propylene glycol, butylene glycol, polyethylene glycol, sorbitol, sodium hyaluronate, and the like.

The thickener is used to maintain the viscosity or to maintain the stability of the product, and it is partially used as a moisturizer and a surfactant. Representative components include guar gum, gelatin, methyl cellulose, alginate, polyvinyl alcohol, and bentonite.

A pigment is a component that gives a color of a product such as a foundation or an eye shadow, and can be largely classified into a tar pigment, a natural pigment and an inorganic pigment.

Preservatives are used to prevent degeneration caused by microorganisms after opening the cosmetics. In total 60 kinds of preservatives that can be used in the country are used. Representative components include parabens, imidazolidinyl urea, phenoxyethanol, and phenonib.

Flavoring agent is a fragrance ingredient, and fragrance is not added to product which is fragrance-free, and fragrance of raw material itself can be made.

Efficacy Ingredients are effective ingredients that perform specific functions such as whitening, improving wrinkles, and feeling of firmness. It is supervised by the Food and Drug Administration to use the appropriate amount to maximize the efficacy without causing skin troubles.

As described above, cosmetics contain various chemical components, so the controversy over the safety of chemical components continues.

Concerns about the safety of some of the surfactants in cosmetics have been raised in several reports, and indeed some ingredients have been found to cause skin problems including dermatitis.

Parabens can cause skin irritation and contact dermatitis and show weak estrogen-like activity in animal studies.

In addition, the synthetic fragrances contain various allergen inducing antigens.

Cosmetics are not absorbed directly into the body like food, but are absorbed through skin and respiration. Therefore, there are not many cases that cause serious health hazards. However, since the age of use of cosmetics has been lowered and the kinds of cosmetics have been diversified, Efforts to secure the safety of cosmetics are becoming increasingly important due to many uses.

Since natural materials have fewer side effects and can be safely used than conventional chemical ingredients while retaining their functionality, studies using natural materials as active ingredients for functional cosmetics are actively under way.

The market for functional cosmetics using natural materials continues to grow. As of 2011, the share of herbal cosmetics in the domestic cosmetics market increased to 24% and occupied 60% of top-line cosmetics sales.

Fermented cosmetics are developed from the materials produced during the fermentation process of natural materials, and fermented the material to produce new effective ingredients, and at the same time, the molecular structure becomes smaller and quickly absorbed to the skin.

Various products are being developed by introducing fermentation techniques to various cosmetic ingredients, and the area of fermented cosmetics is increasing.

The present inventors have invented a cosmetic product which can maintain or improve skin health by producing a cosmetic product containing the fermented composition according to this trend.

Materials used in the present invention will be briefly described below.

Mulberry leaves are mulberry ( Morus alba L.), and the medicinal material with mulberry leaves is called mulberry leaf.

There is almost no odor, and the taste is sweet and spicy. Mulberry leaves contain glutamine, alanine, aspartic acid, tyrosine, serine, glutathione, minerals, dietary fibers and vitamins. Efficiency strengthens blood vessels, stabilizes blood pressure, and eliminates blood purification, heavy metal removal and aging It is known to have efficacy. It is also used for fever, headache, eye congestion, seawater, oozing, skin rash, and has been reported to have antidiabetic action by pharmacological action.

Peony (經药, Paeonia lactiflora ) is a perennial herbaceous herbaceous herbaceous peony peony and peony pupa, with a height of about 1 meter, and it can be divided into peony or vegetable depending on the flower color.

Peony contains ingredients such as aspartic acid and benzoic acid, and headache and toothache to settle. In addition, it removes the blood in the body, smoothes the blood circulation, cleanses the blood, and is effective in improving the female disease. When taking the peony pills steadily, it relaxes the gastrointestinal tract while suppressing the secretion of gastric acid, It is also effective for abdominal pain.

The Eucommia ( Eucommiae) ulmoides Oliver) is a medicinal herb dried bark.

Other names include 仲),,, 중),, 선, 思 사 사, 선,,,,, 棉,, 목 목, 棉,,,,, 선,,,,, 선,,,, and 絲 運 皮, It has a warm temperament.

It treats low back pain caused by lack of function of liver kidneys, knee pain, burning of the body, coldness of lower abdomen, frequent urination, sudden anxiety, uterine bleeding, blood pressure lowering effect, pharmacological action Blood pressure lowering, anti-aging, cholesterol lowering, anti-inflammation, sedation, pain relief, immune control, blood coagulation, uterine contraction, antiallergic and antibacterial activity.

The lotus leaf is called Nelumbo nucifera ), and it is also called lower leaf.

It is known that lotus leaves are rich in vitamins, dietary fiber, minerals, flavonoids, and quercetin, an antioxidant.

The lotus leaves are effective for depression of blood pressure, inhibition of aging due to antioxidative action, prevention of adult diseases, alleviation of dizziness or skin beauty.

On the other hand, Korean Patent Laid-Open No. 10-2010-0048322 discloses a cosmetic composition for prevention of skin aging and antioxidation which contains extract of mulberry leaves as a prior art related to the present invention.

The present invention relates to a cosmetic composition for preventing or treating skin aging, comprising an extract of bamboo bark extract as an active ingredient. The extract of bamboo extract has an antioxidant function capable of protecting skin from oxidative stress induced by ultraviolet rays in epidermal cells (HaCaT) It contains aucubin, which can inhibit the synthesis of matrix metalloproteinase-1 (MMP-1), which is closely related to skin aging. Thus, it has an effect that can be usefully used in functional foods for functionalizing skin, functional cosmetics and drugs Contents are described.

However, the prior art uses only two or three kinds of materials, so that only the efficacy can be known. Moreover, there is a problem that the efficacy can be limited by using aucubin, which is an extract component of mites.

Therefore, it is required to develop cosmetics having antioxidant effect while containing useful physiologically active substances through fermentation.

Korean Patent Publication No. 10-2010-0048322 (2010.05.11)

It is an object of the present invention to provide a skin antioxidant functional cosmetic comprising a fermentation composition obtained by fermenting mulberry leaves, a lotus leaf, a mulberry leaf and a peony root, a mash extract, a herb extract and a red ginseng extract.

The present invention has been made to solve the problems of the present invention by providing mulberry leaves, long leaves, bugs, and peonies with Aspergillus (Rhizopus), Aspergillus ( Aspergillus) oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast, and the like. And a fermented composition prepared by primary fermentation and secondary fermentation with at least one strain selected from the group consisting of actinomycetes (actinomycetes).

The present invention provides a skin antioxidant functional cosmetic comprising a fermentation composition obtained by fermenting mulberry leaves, a lotus leaf, a mulberry leaf and a peony root, an extract of matsugi, a herb extract, and a red ginseng extract.

The present invention relates to a mulberry leaf, a mildew leaf, a mulberry leaf, and a peony pellet, which comprises Aspergillus (Rhizopus), Aspergillus oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast, and the like. And a fermented composition prepared by primary fermentation and secondary fermentation with one or more strains selected from the group consisting of actinomycetes (actinomycetes), to thereby provide a skin antioxidant functional cosmetic.

The cosmetic product containing the fermented composition according to the present invention has a small side effect using a natural product.

The cosmetic composition containing the fermented composition according to the present invention has an effect of maintaining or improving skin health by containing a fermented composition having antioxidant effect.

In addition, the cosmetic composition containing the fermented composition according to the present invention has the effect of decomposing the natural ingredient through the first fermentation and the second fermentation process, thereby easily absorbing the active ingredient into the skin.

FIG. 1 is a graph showing the results of measurement of cell viability after treatment with a natural product extract.
2 is a graph showing the total phenol contents of natural product extracts.
3 is a graph showing DPPH radical scavenging activity of a natural product extract.
FIG. 4 is a diagram showing the molecular formulas of the routine, isoquercetin and quercetin detected by HPLC analysis.
FIG. 5 is a graph showing the results of HPLC analysis of the material before fermentation and after fermentation.
FIG. 6 is a graph showing quantitative results of HPLC analysis of the material before fermentation and after fermentation.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the experimental examples and the reference examples described in the present specification are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents and variations Examples should be understood.

The cosmetic according to the present invention is characterized in that it is prepared by adding a fermentation composition obtained by fermenting mulberry leaf, long leaf, bark, and peony.

In addition, it includes a mashin extract, a red ginseng extract, and a herbal extract. In addition, a moisturizing agent, an emulsifier, a surfactant, a preservative, a perfume, and the like commonly used in cosmetics are used.

The cosmetics according to the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sun cream, a foundation, Such as, but not limited to, soaps, shampoos, rinses, hair treatments, cosmetic wipes, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers, And may be all the goods used for.

Before examining the ingredients and manufacturing process of the cosmetic according to the present invention, the fermented composition contained in the cosmetic according to the present invention will be described.

The fermentation composition can be produced through Reference Example 1 or Reference Example 2. [

Reference Example 1. Method for producing fermentation composition

(1) Sterilization step

Mulberry leaves, peonies, bugs and lotus leaves are washed and sterilized.

Mulberry leaves, peony mosses, bugs, and lotus leaves can be those without any pretreatment, and can be prepared by cutting or grinding. Pretreatment methods such as cutting or grinding can also be selected from a variety of methods.

Preferably, the mulberry leaves, peony mosses, mites, and lotus leaves that have not been subjected to any pretreatment after being cleaned and removed are used.

Sterilization methods include physical methods using heat, ultraviolet rays, radiation or high frequency, and chemical methods using chemical fluids or gases, and a method of sterilization without damaging the active ingredients of mulberry leaves, peanuts, .

(2) Primary fermentation step

The fermentation strain is added to the mulberry leaves, peony moss, mulberry leaves and long leaves which have undergone the above sterilization step, and the first fermentation is carried out. Mulberry leaves, peonies, bugs, and lotus leaves fermented by fermentation strains can increase the extraction rate of medicinal components and can be easily absorbed into the skin.

Fermentation refers to a series of processes in which a complex organic compound is decomposed into a simple organic substance by using an enzyme contained in a microorganism. As the microorganism used in this step, Aspergillus, Rhizopus, Aspergillus oryzae , Aspergillus kawachii , Nattobacillus, Bacillus, Lactic Acid Bacteria, Yeast or Actinomycetes may be selected.

In the present invention, preferably Aspergillus (Rhizopus) or Aspergillus kawachii ), or a mixed microorganism thereof may be used, but the present invention is not limited thereto.

The fermentation may be carried out with mulberry leaves, peony mosses, mosses and orchid leaves, respectively. The mulberry leaves, peony mosses, peonies, mosses and oranges may be mixed and fermented.

According to the design conditions, the weight ratio of the mulberry leaves, peony mosses, peonies, and lotus leaves to the fermentation strain may be set differently, and the added amount of the fermentation strains may be 0.01 to 10 parts by weight based on 100 parts by weight of mulberry leaves, have.

The first fermentation may be performed at a temperature and a period of preferably from 20 to 70 ° C for 1 to 10 days, but the present invention is not limited thereto. In consideration of the characteristics of the fermentation strain, Suffice.

(3) Second fermentation step

The mulberry leaves, peonies, mulberry leaves and lotus leaves that have undergone the primary fermentation step are fermented second.

The fermentation strain, temperature and duration used in the secondary fermentation may be the same as the design conditions in the primary fermentation stage, but are not limited thereto.

Preferably, white germ can be used for the primary fermentation and can be used for the secondary fermentation.

(4) Extraction step

The fermented products of mulberry leaf, peony, mugwort and lotus leaf which have undergone the primary and secondary fermentation stages are dried and mixed and hydrothermally extracted.

Drying methods include natural drying, hot air drying, low temperature drying, freeze drying, far-infrared drying, daylight drying, vacuum drying, and polyethylene (PE) house drying.

Preferably, a natural drying method may be used, but not limited thereto, and it is sufficient to remove moisture from mulberry leaves, peony mosses, bugs, and lotus leaves.

At this time, the dried mulberry leaves, peony moss, moss, and lotus leaf can be used after being pulverized using a powder machine.

The fermented product of dried mulberry leaves, peonies, mulberry leaves and lotus leaves is mixed and hot water extracted to prepare a mixed extract.

At this time, the weight ratio can be variously set, but preferably the fermented product of mulberry leaf, peony, bark, and lotus leaf can be mixed at a weight ratio of 0.5-1.5: 0.5-1.5: 0.5-1.5: 0.5-1.5.

Various extraction methods such as hot water extraction method, solvent extraction method, steam distillation method, carbon dioxide supercritical extraction method, steam distillation method, microwave process extraction method and percolation extraction method can be used as a method of extracting mulberry leaves, peonies, , Preferably hydrothermal extraction, but not limited thereto

The temperature and time of the hot water extraction may be designed to suit the state of the fermented product, and preferably, it may be heated and extracted at 90 to 120 ° C for 1 to 3 hours.

At this time, when the hot water extraction condition is less than the range defined above, there is a possibility that the respective effective components contained in the fermented product may not be extracted sufficiently. If the hot water extraction condition exceeds the range defined above There is a possibility that each of the effective components contained in the fermentation product is destroyed and the efficacy thereof is lost.

(5) Filtration and concentration step

The combined extracts are filtered and concentrated.

The filtration methods include atmospheric pressure filtration, suction filtration, reduced pressure filtration, ultrafiltration, microfiltration and reverse osmosis filtration, and the like.

The filtration time, temperature and the number of times can also be set variously.

Filtration may be carried out using the methods listed above or other methods, and may be performed using two or more methods.

When performing microfiltration or ultrafiltration, the filtration membrane pore size may preferably be 3 μm, but is not limited thereto.

The concentration method includes, for example, freezing concentration, reduced pressure concentration, membrane concentration, vacuum concentration, heat concentration and evaporation concentration. Preferably, the concentration under reduced pressure can be performed, but is not limited thereto.

Two or more species may be used for the concentration method.

The concentration temperature can be set variously. But it is not limited thereto. The concentration time and the number of times can also be variously set.

Through the above steps, a fermentation composition containing mulberry leaves, lotus leaves, bugs, and peanuts as an active ingredient can be produced.

Reference Example  2. Manufacturing Method of Fermentation Composition

(1) Sterilization step of fermentation material

Was carried out in the same manner as described in Reference Example 1.

(2) Primary fermentation step

Was carried out in the same manner as described in Reference Example 1.

(3) Aging step

The fermented products of mulberry leaves, peonies, mulberry leaves and long leaves which have undergone the first fermentation step are respectively aged.

According to the design conditions, the aging temperature and duration may be aged for 10 to 90 days at 15 to 35 DEG C, but are not limited thereto.

The aging step may be performed after the first fermentation, but the present invention is not limited thereto, and the fermentation step may be performed after the first fermentation and the second fermentation step.

(4) Second fermentation step

Was carried out in the same manner as described in Reference Example 1.

(5) Ultrasonic wave breaking and extraction step

The fermented product of mulberry leaf, peony, bamboo, and lotus leaf after the second fermentation step is ultrasonicated.

The ultrasonic disruption time can be set to 15 to 60 minutes, and can be variously set according to the conditions.

The ultrasonic wave crusher has the effect of inducing molecular motion due to the unique cavitation of the ultrasonic wave and has the effect of improving the reactivity when performing the processes such as extraction and concentration by acting as activation energy in the molecular motion, When the sample is crushed using an ultrasonic wave crusher as an apparatus that has been introduced, the production rate of the protein contained in the sample can be improved.

In order to efficiently extract fermented water components of mulberry leaves, peony mosses, mulberry leaves and lotus leaves using the characteristics of the ultrasonic wave crusher as described above, ultrasonic disruption is performed on the fermentations of mulberry leaves, peony mosses, mosses and long leaves.

Extraction after ultrasonic disruption was carried out in the same manner as described in Reference Example 1.

(6) Filtration and concentration step

Was carried out in the same manner as described in Reference Example 1.

Reference Example  3. Functional effects of fermentation composition

3-1. Cell viability measurement

1) Experimental process

The toxicity of the natural extract was determined by measuring the cell viability using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide) assay.

The MTT method can determine the cell survival rate by measuring the amount of MTT converted to formazan by the dehydrogenase contained in the mitochondria of living cells.

The cell line used was RAW 264.7 cells and cultured in DMEM (high glucose) medium supplemented with 10% FBS, penicillin (100 U / ml) and streptomycin (100 ug / ml)

① 96-well plate and then preparing six injected into the medium in each plate was inoculated 100 uL to be 5 × 10 ⁴ cell / well of RAW 264.7 cell, incubated in 5% CO _2, 37 ℃ incubator for 24 hours Respectively. One of the six plates was set as a control group and separated from the other plate.

(2) Natural extracts (200 μg / ml) were added to 5 plates except the plate set as a control group in the culture plate for 24 hours.

③ 15 μL of MTT solution was added to all wells and reacted at 37 ℃ incubator.

④ After 4 hours, insert the solubilizing solution, seal it to prevent the water from evaporating, leave it at 37 ° C for 12 hours, measure the absorbance with a microplate spectrophotometer (Molecular dynamics, USA) with 570 nm and 630 nm as test and reference wavelength, respectively Respectively.

⑤ The OD of the control and experimental groups was compared and the cell viability was measured. The cell survival rate was calculated based on the following formula (1).

2) Experimental results

FIG. 1 is a graph showing the results of measurement of cell viability after treatment with a natural product extract.

As a result of the experiment, natural extracts showed cell viability similar to or higher than that of the control group, and thus it can be confirmed that there is no cytotoxicity.

3-2. Total phenol content measurement

The antioxidant activity of the fermented composition was confirmed by measuring the total phenol content of the fermented composition.

1) Experimental process

The phenolic substance reacts with phosphomolybic acid to show a blue color, which is measured by the Folin-Denis method.

1) Extract solution of 100 mg / mL was prepared by adding 1 mL of PBS buffer per 100 mg of natural product extract and used as a sample solution.

② Add 0.33 mL of sample, 0.16 mL of Folin-Denis and 0.3 mL of NO2CO3 to 2.5 mL of distilled water, and measure the absorbance at 760 nm in a dark room for 30 min. The standard was tannic acid.

2) Experimental results

2 is a graph showing the total phenol contents of natural product extracts.

The total phenol contents in hot water extracts were higher in order of mulberry leaves, lotus leaves, peonies, and mulberries. In the extracts of mulberry leaves, total phenol contents were higher in the order of peonies, lotus leaves, mulberry leaves and mulberry leaves.

It was also found that the total phenol content increased in the group fermented with white rice, white rice, yeast or koji compared to the nonfermented rice (RAW).

3-3. DPPH Radical Scatters  Measure

The antioxidant activity of the fermented composition was confirmed by measuring the DPPH radical scavenging ability after the fermentation composition treatment.

1) Experimental process

① Extract solution of 100 mg / mL was prepared by adding 1 mL of methanol per 100 mg of natural product extract and used as a sample solution.

② 150 μL of 1.5 × 10 ^-4 mM DPPH solution was added to 50 μL of the sample solution, and the absorbance was measured at 515 nm 30 minutes later.

③ The radical scavenging ability (%) was calculated by the following equation

Free radical scavenging effect = (Abs _DPPH- Abs _sample ) / Abs _DPPH占 100

2) Experimental results

3 is a graph showing DPPH radical scavenging activity of a natural product extract.

The results showed that the antioxidant activity of hot water extract was stronger in order of peony, mulberry leaf, mulberry leaf, and mulberry leaf. In particular, the leaves and peonies were superior to ascorbic acid used as a control group. Peanut extracts showed the highest antioxidant activity in alcohol extracts.

3-4. Glycoside decomposing activity

High performance liquid chromatography (HPLC) was used to confirm the effect of glycosidation upon fermentation.

1) Experimental process

(1) 10 mL of distilled water was added to each natural substance extract, and the resultant was filtered at 0.45 μm and measured using HPLC (Agilent 1260, Agilent Technologies, USA).

② HPLC analysis was carried out using Agilent poroshell 120EC-C18 (3.0 mm × 50 mm, 2.7 μm) column. The flow rate and column temperature of the mobile phase were 0.8 ml / min and 35 ℃, respectively. The wavelength was analyzed at 203 nm.

2) Experimental results

Analysis of one of the flavonoid components, rutin, isoquercetin and quercetin, confirmed the effect of glycosidation by fermentation. Here, quercetin is known to prevent the aging of the skin by removing active oxygen in the body.

FIG. 4 is a diagram showing the molecular formulas of the routine, isoquercetin and quercetin detected by HPLC analysis.

FIG. 5 is a graph showing the results of HPLC analysis of the material before fermentation and after fermentation.

FIG. 6 is a graph showing quantitative results of HPLC analysis of the material before fermentation and after fermentation.

As a result of the experiment, it was confirmed that the amount of rutin and isoquercetin contained in the material after fermentation was decreased and the content of quercetin was increased compared with before fermentation.

This means that the routine and isoquercetin are converted to quercetin, meaning that the glycoside component of the material has been degraded through fermentation.

Therefore, it is predicted that the size of the flavonoid component of the fermented material becomes smaller and the absorption becomes easier.

3-5. Yield measurement of extract

The extraction efficiency according to the production method was examined by calculating the yield of the fermented composition according to Reference Examples 1 and 2.

1) Preparing the experiment

As the samples used in the experiments, the extracts according to Reference Examples 1 and 2 and the extracts obtained by directly extracting the plant material without fermentation were used as a comparative example.

2) Experimental process

Each extract was filtered with a vacuum filter, concentrated, lyophilized, and then the weight of the powder was measured to calculate the yield.

The yield was calculated using the following equation.

3) Experimental results

Table 1 shows the yield of the extract according to the production method.

Reference Example 1 Reference Example 2 Comparative Example Yield (% w / w) 11.34 17.81 7.07

As a result, the yield of the extract according to Reference Example 1 was 11.34%, the extract according to Reference Example 2 was 17.81%, and the extract according to Comparative Example 2 had a yield of 7.07%.

That is, it can be seen that the extraction yield of the material is increased by aging between the first fermentation and the second fermentation and extracting after ultrasonic disruption.

In summary, it can be confirmed that the fermented composition fermented with mulberry leaves, leafy leaves, mites, and peonies has antioxidant, anti-inflammatory and whitening effects, and cosmetics containing the fermented composition also have the above- .

The cosmetics according to the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sun cream, a foundation, , A soap, a shampoo, a rinse, a hair treatment, a cosmetic wet tissue, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

The ingredients and preparation of cosmetics containing the fermented composition will be described in detail as follows.

Manufacturing example  1. Lotion containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycol, 2 to 4 parts by weight of glyceryl glucoside, And 0.1 to 7 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.15 parts by weight of carbomer and 0.01 to 0.15 part by weight of xanthan gum are added to the dissolved 1) solution and then dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 0.60 to 1.00 parts by weight of cetostearyl alcohol, 1.00 to 1.40 parts by weight of glyceryl stearate SE, 0.5 to 1.5 parts by weight of glyceryl stearate, 0.5 to 1.0 part by weight of Polysorbate 60, 0.3 to 0.7 part by weight of beeswax, 0.3 to 0.7 part by weight of Sorbitan Stearate, 5 to 7 parts by weight of Mineral Oil, 2 to 4 parts by weight of Macadamia Integrifolis Seed, 1 to 3 parts by weight of Caprylic / Capric Triglyceride, 0.03 to 0.07 part by weight of Tocopheryl Acetate and 0.03 to 0.07 part by weight of Dimethyl Silicone 0.20 to 0.60 parts by weight of oil) is melted by heating at 70 to 90 ° C, and then added to the above 2).

4) After charging, emulsify with homomixer at 3,000 rpm for 5 ~ 20 minutes.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.15 parts by weight of triethanolamide are added.

6) After the mixture is cooled to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added.

7) The fermented composition according to any one of the above items 1 to 5, wherein the fermented composition is 0.1 to 5 parts by weight, the herb extract is 0.01 to 0.05 parts by weight, the horseradish extract is 0.001 to 0.02 parts by weight, the red ginseng extract is 0.01 to 1 part by weight, the fragrance is 0.03 to 0.07 parts by weight, 0.5 to 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  2. Toner containing the fermentation composition

1) 70 to 80 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 part by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycol, 2 to 4 parts by weight of glyceryl glucoside, And 0.1 to 7 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.15 part by weight of carbomer is added to the dissolved solution (1), and the mixture is dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) Add 3 to 7 parts by weight of water aqua and 0.10 to 0.15 parts by weight of triethanolamide to the above 2).

4) 0.30 to 0.70 parts by weight of methylphenyl polysiloxane and 0.1 to 0.5 parts by weight of polysorbate 80 are weighed into the above 3).

5) 0.20 to 0.60 parts by weight of phenoxyethanol is added to 4) and mixed.

(6) The method of (5), wherein the fermented composition comprises 0.1 to 5 parts by weight of the herbal extract, 0.01 to 0.05 parts by weight of the herbal extract, 0.001 to 0.02 part by weight of the extract of marigold, 0.01 to 1 part by weight of the extract of red ginseng, 0.01 to 0.05 part by weight of fragrance, 7 parts by weight, and the mixture is mixed to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  3. Essence containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, , 1.5 to 2.5 parts by weight of niacinamide, 1.5 to 2.5 parts by weight of butylene glycols, and 1 to 3 parts by weight of glyceryl glucoside (weight ratio), 0.03 to 0.05 parts by weight of adenosine, 5 to 7 parts by weight of glycerine and 2 to 4 parts by weight of polyethylene glycol are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.01 to 0.10 parts by weight of carbomer and 0.10 to 0.15 part by weight of xanthan gum are added to the dissolved 1) solution and then dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 0.1 to 1.0 part by weight of Macadamia Integrifolia Seed and 0.05 to 0.15 part by weight of Methyl Phenyl Polysiloxane are metered into the above 2).

4) After cooling the mixture to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added and mixed.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.30 parts by weight of triethanolamide are added to 4).

6) A method for producing a fermented composition as described in 5) above, wherein the fermented composition is 0.1-5 parts by weight, the herb extract is 0.01-0.05 parts by weight, the gherkin extract is 0.001-0.02 parts by weight, the red ginseng extract is 0.01-1 parts by weight, the fragrance is 0.03-0.07 parts by weight and the hyaluronic acid 0.5 To 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

Manufacturing example  4. Cream containing fermented composition

1) 60 to 70 parts by weight of water aqua, 0.02 to 0.04 parts by weight of disodium EDTA, 0.05 to 0.15 parts by weight of allantoin, 0.04 to 0.06 parts by weight of dipotassium glycyrrhizate, 0.03 to 0.05 part by weight of adenosine, 1.5 to 2.5 parts by weight of Niacinamide, 1.5 to 2.5 parts by weight of Butylene Glycol, 3 to 5 parts by weight of Glyceryl Glucoside, And 2 to 4 parts by weight of glycerine are weighed and dissolved by heating at 70 to 90 ° C.

2) 0.05 to 0.15 parts by weight of carbomer and 0.01 to 0.05 part by weight of xanthan gum are added to the solution 1) and dispersed.

Carbomers are used as emulsifying stabilizers and thickeners, and the viscosity of cosmetics can be controlled according to the amount added.

3) 3 to 4 parts by weight of cetostearyl alcohol, 1 to 3 parts by weight of glyceryl stearate, 1 to 2 parts by weight of Polysorbate 60, 2 to 3 parts by weight of sorbitan 0.3 to 0.7 parts by weight of Sorbitan Stearate, 5 to 7 parts by weight of Mineral Oil, 2 to 4 parts by weight of Macadamia Integrifolis Seed, Caprylic / Capric triglyceride, 1 to 3 parts by weight of triglyceride, 0.03 to 0.07 part by weight of tocopheryl acetate, 0.20 to 0.60 part by weight of dimethyl silicone oil, 0.1 to 1.0 part by weight of microcrystalline wax, 1 to 2 parts by weight of stearic acid and 0.5 to 1.5 parts by weight of cyclopentasiloxane and dimethicone / dimethicone / vinyl dimethicone crosspolymer are weighed and dissolved by heating at 70 to 90 ° C., ).

4) After charging, emulsify with homomixer at 3,000 rpm for 5 ~ 20 minutes.

5) 3 to 7 parts by weight of water aqua and 0.10 to 0.20 parts by weight of triethanolamide are added.

6) After the mixture is cooled to 35 to 45 ° C, 0.20 to 0.60 parts by weight of phenoxyethanol is added.

7) The fermented composition according to any one of the above items 1 to 5, wherein the fermented composition is 0.1 to 5 parts by weight, the herb extract is 0.01 to 0.05 parts by weight, the horseradish extract is 0.001 to 0.02 parts by weight, the red ginseng extract is 0.01 to 1 part by weight, the fragrance is 0.03 to 0.07 parts by weight, 0.5 to 1.5 parts by weight, and the mixture is stirred to complete a cosmetic product.

In this case, herbal extracts may be Swiss Alpine Herb Extract, which contains arnica flower extract, Gentiana extract, wormwood extract and Mugwort extract.

The cosmetics manufactured afterward can be used by being housed in containers after cooling and aging.

On the other hand, the present inventors conducted safety tests on the cosmetics according to Preparation Examples 1 to 4 and confirmed the results shown in Table 2 below.

Production Example 1. Lotion Production Example 2 Toner Production Example 3. Essence Production Example 4. Cream Appearance and Appearance White lotion phase Milky liquid
(Sensory evaluation) Opaque viscous liquid White creamy top Viscosity 6,000 ~ 8,000 2,500 ~ 3,500 15,000 ~ 20,000 pH 5.5 to 6.5 5.5 to 6.5 6.0 to 7.0 5.5 to 6.5 microbe General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) General bacterium 0 CFU / ml (not detected)
Pathogenic fungi 0CFU / ml (not detected) Common bacteria
0CFU / ml (not detected)
Pathogenic bacteria
0CFU / ml (not detected)

Claims (5)

The present invention relates to a skin antioxidant functional cosmetic composition containing a fermented composition fermented with mulberry leaf,
The fermentation composition is firstly fermented at 20 to 70 ° C for 1 to 10 days using Aspergillus kawachii , and after the first fermentation, the fermented composition is cultivated at 20 to 70 ° C using Aspergillus (Rhizopus) Lt; RTI ID = 0.0 > 70 C < / RTI > for 1 to 10 days,
Aspergillus kawachii or Aspergillus or Rhizopus is added in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of mulberry leaves,
Wherein the cosmetic comprises 0.1 to 5 parts by weight of a fermented composition, 0.001 to 0.02 parts by weight of a crude extract, 0.01 to 0.05 parts by weight of a herbal extract, and 0.01 to 1 part by weight of a red ginseng extract.
delete delete delete The method according to claim 1,
The cosmetic may be at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a sunscreen, a foundation, Wherein the composition is one of a shampoo, a rinse, a hair treatment, a cosmetic wipes, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser.

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