KR102315713B1 - External composition for inhibiting hair loss and/or promoting hair growth - Google Patents

Abstract

The present invention relates to an external composition containing the riboflavin 5'-phosphate or an acceptable salt thereof as an active ingredient, which is safe for the human body and has the effect of preventing hair loss and/or promoting hair growth.

Description

External composition for preventing hair loss and/or promoting hair growth {External composition for inhibiting hair loss and/or promoting hair growth}

The present invention relates to a composition for preventing hair loss and/or promoting hair growth, and to a composition for external application for preventing hair loss and/or promoting hair growth containing a component inducing hair growth promotion.

In the process of increasing interest in beauty as the times change, hair occupies an important part. Hair is a thin, keratinized structure produced on the surface of the skin. In addition to acting as a cushion against external shock, it protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and discharges heavy metals such as arsenic, mercury and zinc that are harmful to the body. Aspects are also highlighted. However, there are an increasing number of people complaining of hair loss due to various causes, including changes in diet and increased internal and external stress. It is known that genetic factors act as the main cause of hair loss, but as mentioned above, increase in social stress, environmental pollution, frequent perms and dyeing, and incorrect scalp care are also emerging as causes of hair loss.

Human hair is about 100,000 to 150,000, and each hair has a different growth cycle. The first generated hair goes through the growth phase, then goes into the degeneration and resting phase, and then goes through a cycle of falling out and then entering the growth phase again. The hair growth cycle consists of three stages: the anagen stage, in which hair grows most actively, the catagen stage, in which hair degeneration begins, and the telogen stage, in which hair growth stops or enters rest. classified as

In general, alopecia refers to an increase in the number of abnormal hair loss due to the shortening of the proportion of hair in the growth phase and more hair in the degenerative phase or telogen phase among these cycles. Hair loss is a normal phenomenon, but compared to a normal person having a lot of hair in the growth phase, a person with normal alopecia has a lot of hair in a telogen state, so visible hair loss is shown. A characteristic of people with alopecia is the miniaturization of their hair. As hair loss progresses, the period of growth is shortened, the transition to the catagen and resting phases is promoted, and the volume of the dermal papilla that follows becomes smaller and the hair follicles become smaller. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting state to quickly go to the growth phase and to extend the shortened growth phase.

As mentioned above, the causes of hair loss include male hormone action, excessive sebum secretion, poor blood circulation, decreased scalp function due to peroxides or bacteria, genetic factors, aging, stress, etc. have been discussed. However, until now, the clear cause of hair loss has not been elucidated, and it is known that very complex actions are intertwined.

For these various and complicated causes of hair loss, the currently known hair loss prevention products are active ingredients that promote blood circulation, strengthen hair root function, moisturize the scalp, prevent dandruff, antioxidant effect, hair growth period extension effect, and suppress the action of male hormones. It contains ingredients that do this, but there is still no one with a clear effect in terms of effectiveness, and the problem of side effects has been raised.

Currently, minoxidil (2,4-diamino-6-piperidinopyrimidine-3-oxide) is the only hair loss treatment approved by the US Food and Drug Administration (FDA). It was unsatisfactory at 13% (J Am Acad Dermatol. 2002. 47(3)), and there was a disadvantage that the effect on the bangs was inferior. In addition, it is known that side effects such as contact dermatitis or hirsutism such as scalp pruritus, erythema and keratin may occur when minoxidil is used excessively (J Korean Med Assoc 2013 January; 56(1): 45-54).

Therefore, there is a need for development of a composition that can effectively act on hair growth or hair growth promotion while improving the problems such as side effects or precautions for use of the existing hair loss treatment agents, while being safe for the human body.

The present invention was created to solve the problems of the prior art as described above, and an object of the present invention is to provide an external composition for preventing hair loss and/or promoting hair growth containing riboflavin 5'-phosphate or an acceptable salt thereof as an active ingredient will do

The present invention provides an external composition for preventing hair loss and/or promoting hair growth containing riboflavin 5'-phosphate represented by the following Chemical Formula 1 or an acceptable salt thereof as an active ingredient.

Acceptable salts may refer to salts that are formed according to various methods for preparing riboflavin 5'-phosphate, or that can be prepared through methods for preparing salts known in the art. Preferably, the salt may be a pharmaceutically acceptable salt. The acceptable salt may include a salt of an acidic or basic group that may be present in the riboflavin 5'-phosphate. The acceptable salt may be, for example, sodium, calcium, potassium salt, sulfate, hydrogen sulfate, phosphate, or hydrogen phosphate of riboflavin 5'-phosphate.

Specifically, the acceptable salt of riboflavin 5'-phosphate used in an embodiment according to the present invention may be sodium riboflavin 5'-phosphate represented by the following formula (2). In the present invention, riboflavin 5'-phosphate sodium may also be referred to as riboflavin 5'-phosphate sodium, SRP (Sodium Riboflavin 5'-Phosphate), or RPS (Riboflavin 5'-phosphate sodium).

In the present invention, riboflavin 5'-phosphate or an acceptable salt thereof may be obtained by chemical synthesis or separation from a natural material, or may be purchased and used commercially at home and abroad.

When the riboflavin 5'-phosphate sodium used in the present invention is chemically synthesized, it can be obtained by reacting riboflavin in an excess of phosphoryl chloride and then adding sodium hydroxide.

When riboflavin 5'-phosphate sodium commercially available in the present invention was purchased, it was purchased from Sigma Aldrich and used.

In the present invention, the composition for topical application containing riboflavin 5'-phosphate or an acceptable salt thereof as an active ingredient has an effect of preventing hair loss and promoting hair growth.

In the present invention, hair loss may occur due to male hormone action, excessive sebum secretion, poor blood circulation, decreased scalp function due to peroxide or bacteria, genetic factors, aging, stress, etc., and prevention of hair loss according to the present invention and/or The composition for external application for promoting hair growth may exhibit an excellent effect on hair loss due to the various causes. Preferably, the composition for external application for preventing hair loss and/or promoting hair growth according to the present invention can exhibit an excellent effect on hair loss due to the action of male hormones.

In the present invention, the mechanism of hair loss by male hormone action is as follows. When male hormone binds to the male hormone receptor and a signal transduction process occurs, TGF-β production is promoted in cells including hair follicle cells. At this time, intracellular signaling occurs as TGF-β binds to the TGF-β receptor present on the cell surface, and as a result, the expression of various hair loss-related genes in which the TGF-β receptor acts in the cell increases, and ultimately hair follicles. It causes apoptosis of stromal cells and outer sheath keratinocytes. As a result, since it induces hair follicles in the anagen phase to the degenerative phase, an increase in the amount of TGF-β induces apoptosis of hair matrix cells and outer sheath muscle cells. It can easily fall out and result in thin and short hair.

As used herein, the term “prevention of hair loss” means preventing or weakening the phenomenon of hair falling out from the hair follicles or scalp.

As used herein, the term "promoting hair growth" means increasing the rate or amount of hair production, and includes increasing the thickness of the generated hair.

As used herein, the term "composition for external application" refers to a composition for administration by direct application or spraying to the skin, and preferably refers to a composition for transdermal administration. The term "administration" used in the present invention means introducing the composition according to the present invention to a target tissue using a conventional method known in the art to which the present invention pertains.

In the present invention, the riboflavin 5'-phosphate or an acceptable salt thereof may inhibit male hormone activity.

In the present invention, "male hormone" refers to androgens that affect the growth and development of the male reproductive system, and which can induce the hair loss mechanism described above by binding to the male hormone receptor. The male hormone may be, for example, an androstane derivative such as testosterone, and dihydroxytestosterone, androsterone, or dihydroepiandrosterone produced from the derivative through a metabolic reaction, but is not limited thereto.

In the present invention, the riboflavin 5'-phosphate or an acceptable salt thereof may inhibit male hormone receptor protein expression.

In the present invention, "male hormone receptor" refers to a protein that provides binding to male hormone, and can regulate the degree of transcription of a specific gene by providing direct binding to a DNA sequence such as a hormone response element (HRE). .

In the present invention, the riboflavin 5'-phosphate or an acceptable salt thereof may inhibit the expression of follicle degenerative factors. In regulating the hair growth cycle, the activity of outer root sheath cells including dermal papilla cells and hair follicle stem cells constituting hair follicles and various cytokines and growth factors produced by these cells play an important role. is known

In the present invention, "hair follicle degenerative factor" is a growth factor inducing the degenerative phase of hair follicles, and they may play an important role in inhibiting hair follicle growth and apoptosis. The hair follicle degenerative factor may be, for example, one or more selected from the group consisting of Dkk-1, TGFb-1 and EGF.

In the present invention, the riboflavin 5'-phosphate or an acceptable salt thereof may be contained in an amount of 0.0001 to 50% by weight based on the total weight of the composition for external use. Preferably, the riboflavin 5'-phosphate or an acceptable salt thereof may be contained in an amount of 0.0001 to 20% by weight, most preferably 0.001 to 10% by weight, based on the total weight of the composition for external use.

The composition for external application for preventing hair loss and/or promoting hair growth of the present invention is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream , hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair It may be in the form of a dressinger, hair lacquer, hair moisturizer, hair mousse or hair spray.

When the composition of the present invention is formulated in a liquid form, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, water, ethanol, castor oil, glycerin, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbate. It may be a fatty acid ester such as hearth, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a paste, cream or gel, the composition of the present invention may include a carrier for the active ingredient. The carrier is, for example, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivatives such as tracanth, hydroxyethyl, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, cetostearyl alcohol or chloride It may be stearyltriethylammonium, but is not limited thereto. These may be used alone or in combination of two or more.

When the composition of the present invention is formulated as a powder or spray, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, but is not limited thereto. These may be used alone or in combination of two or more. When the composition of the present invention is formulated as a spray, a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether may be further included.

When the composition of the present invention is formulated as a soap, the composition of the present invention may include a carrier for the active ingredient. The carrier may be, for example, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc., but is limited thereto. it's not going to be These may be used alone or in combination of two or more.

The composition of the present invention may further include additives commonly used for external preparations. The additive may be, for example, purified water, a surfactant, a humectant, a lower alcohol, a chelating agent, a disinfectant, an antioxidant, a preservative, a color, or a fragrance, but is not limited thereto. These may be used alone or in combination of two or more.

The composition for external application for preventing hair loss and/or promoting hair growth according to the present invention is harmless to the human body and has an excellent hair growth promoting effect compared to minoxidil and riboflavin.

The composition for preventing hair loss and / or promoting hair growth according to the present invention shortens the cycle from the resting phase to the anagen phase during the hair growth cycle through inhibition of male hormone activity, inhibition of male hormone receptor protein expression, and inhibition of hair follicle degenerative factor expression, thereby preventing hair growth. It has the effect of promoting and preventing hair loss by delaying the transition to the degenerative phase.

1 is a graph showing the male hormone receptor transcriptional activity according to the concentration of sodium riboflavin 5'-phosphate in Example 1.
2 is a Western blot showing the expression of male hormone receptor protein according to the concentration of sodium riboflavin 5'-phosphate in Example 2.
3 is a Western blot showing the expression of male hormone receptor protein induced by male hormone according to the concentration of sodium riboflavin 5'-phosphate in Example 2. FIG.
4 is a Western blot showing the expression of follicle degenerative factors according to the concentration of sodium riboflavin 5'-phosphate in Example 3. FIG.

The objects, specific advantages and novel features of the present invention will become more apparent from the following detailed description and preferred embodiments taken in conjunction with the accompanying drawings. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples. In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

Example 1. Efficacy of riboflavin 5'-phosphate sodium to inhibit androgen activity

The following experiment was performed to confirm the efficacy of riboflavin 5'-phosphate sodium to prevent hair loss and promote hair growth. Riboflavin 5'-phosphate sodium used in this Example was purchased from Sigma Aldrich and used.

A stable cell line constructed by permanently transfecting androgen receptor-positive 22Rv1 human prostate cancer cell line with Pgl4.36-MMTV-Luc vector containing two androgen response factors and a firefly luciferase reporter gene (stable cell line) was used. The stabilized cell line was subcultured using RPMI1640 and 10% Fetal bovine serum (Gibco BRL, Gaithersburg, MD, USA). For the transcriptional activity test method, 25,000 cells were seeded in each well in a 96-well plate, and the culture medium was replaced with phenol-red free RPMI1640 containing 5% charcoal-stripped fetal bovine serum. Thereafter, after culturing for 48 hours in an incubator at 37° C., riboflavin 5'-phosphate sodium was treated with 1 nM DHT (Dihydrotestosterone), incubated for 24 hours again, and added to DHT using the Luciferase assay system (Promega). The degree of inhibition of increased Luciferase activity was measured. The measurement result was 100% of the Luciferase activity (luminescence) value increased by 1 nM DHT treatment, indicating a value inhibited by riboflavin 5'-phosphate sodium. As a positive control, bicalutamide (casodex), a non-steroidal anti-androgen, was used, and cell activity was exhibited in parallel with CCK-8 assay to confirm cytotoxicity caused by each material treatment. CCK-8 reagent was treated at a concentration of 1:10 in each culture medium and cultured for 2 hours, and absorbance of each well was measured at 450 nm after 2 hours. All experiments were repeated 3 times, and absorbance values were calculated as an average, and the value of the 1 nM DHT-treated group was set to 100%, indicating the values of the remaining treatment groups.

The experimental results are shown in Tables 1 and 2 below.

treatment group treatment concentration male hormone activity
(% of 1 nM DHT) untreated group - 0 DHT 1 nM 100.0 bicalutamide 20 μM 3.5 1 nM DHT bicalutamide 20 μM 28.7 Riboflavin 5'-phosphate sodium 1.25 ppm 88.5 2.5 ppm 90.0 5 ppm 82.9 10 ppm 75.0 20 ppm 51.3

treatment group treatment concentration cell activity
(% of 1 nM DHT) untreated group - 91.6 DHT 1 nM 100.0 bicalutamide 20 μM 92.6 1 nM DHT bicalutamide 20 μM 92.1 Riboflavin 5'-phosphate sodium 1.25 ppm 108.1 2.5 ppm 110.7 5 ppm 120.0 10 ppm 120.4 20 ppm 122.3

1 is a 1 nM DHT treatment group in Tables 1 and 2, and 1.25 ppm, 2.5 ppm, 5 ppm, 10 ppm and 20 ppm of sodium riboflavin 5'-phosphate, respectively, based on the 1 nM DHT weight for the treatment group. It is a graph showing testosterone activity and cell activity of the treated group. The line in the middle of the bar graph showing cell activity represents the standard deviation.

As shown in Table 1 and FIG. 1, it was found that the Luciferase activity was inhibited by treatment with riboflavin 5'-phosphate sodium, and the Luciferase activity was significantly inhibited at a treatment concentration of 10 ppm or more.

The above experimental results demonstrate the efficacy of riboflavin 5'-phosphate sodium to inhibit androgen activity.

Example 2. Inhibition of expression of male hormone receptor protein of riboflavin 5'-phosphate sodium

The following experiment was performed to confirm the efficacy of riboflavin 5'-phosphate sodium to prevent hair loss and promote hair growth. Riboflavin 5'-phosphate sodium used in this Example was purchased from Sigma Aldrich and used.

The LNCaP cell line, a male hormone receptor-positive prostate cancer cell line, was subcultured using RPMI1640 and 10% Fetal bovine serum. The cell lines were seeded by 500,000 each in a 60 mm cell culture dish, and then cultured in an incubator at 37° C. for 48 hours. Thereafter, riboflavin 5'-phosphate was treated with 10 ppm and 20 ppm of sodium, and after 24 hours, each cell line was obtained and Western blot was performed to confirm the protein expression level. GADPH was used as a control for comparison of protein expression level.

The experimental results are shown in Table 3 below.

treatment group treatment concentration Testosterone receptor protein expression level untreated group - 100 Riboflavin 5'-phosphate sodium 10 ppm 64.3 20 ppm 53.0

Figure 2 shows the expression level of male hormone receptor protein (AR) in the untreated group and the group treated with riboflavin 5'-phosphate sodium at 10 ppm and 20 ppm, respectively, and GADPH expression level as a control group in Table 3; blot result.

As can be seen in Figure 2, it can be confirmed that the expression level of the male hormone receptor protein is remarkably reduced according to the treatment concentration of the riboflavin 5'-phosphate sodium treatment group.

Additionally, western blot results when 1 nM DHT was treated with LNCaP cell line in hormone starvation state and treated with sodium riboflavin 5'-phosphate were shown in FIG.

As can be seen in Figure 3, according to the treatment concentration of the riboflavin 5'-phosphate sodium treatment group, it can be confirmed that the expression level of the male hormone receptor protein induced by 1 nM DHT treatment decreases.

The above experimental results demonstrate that riboflavin 5'-phosphate sodium can inhibit the expression of testosterone receptor protein in both cases induced by androgen-induced or not.

Example 3. Efficacy of riboflavin 5'-phosphate sodium follicle degenerative factor expression inhibition

The following experiment was performed to confirm the efficacy of riboflavin 5'-phosphate sodium to prevent hair loss and promote hair growth. Riboflavin 5'-phosphate sodium used in this Example was purchased from Sigma Aldrich, and human dermal papilla cells (hDPCs) were purchased from PromoCell.

Follicle Dermal Papilla Cell Growth Medium (Follicle Dermal Papilla Cell Growth Medium; Promo cell) containing human-derived dermal papilla cells as a culture medium additive (Supplement Mix; Promo cell, Heidelberg, Germany), penicillin 100 unit/mL and streptomycin 100 µg/mL , Heidelberg, Germany) was subcultured at 37° C. and 5% CO _{2 conditions.} After seeding 1 million cultured human-derived dermal papilla cells in a 100 mm cell culture dish, they were cultured in an incubator at 37° C. for 48 hours. Thereafter, riboflavin 5'-phosphate sodium was treated with 10 ppm and 20 ppm, and after 24 hours, each cell was obtained and Western blot was performed to confirm the expression level of the follicle degenerative factor. GADPH was used as a control for comparison of protein expression level.

The experimental results are shown in Table 4 below.

treatment group treatment concentration Dkk-1 expression level untreated group - 100 Riboflavin 5'-phosphate sodium 10 ppm 60.4 20 ppm 51.6

4 is a western showing the expression level of Dkk-1, a hair follicle degeneration factor, and GADPH, a control group, in the untreated group and in the group treated with 10 ppm and 20 ppm of riboflavin 5'-phosphate sodium, respectively, in Table 4; blot result.

As can be seen in Figure 4, it can be confirmed that the expression level of the male hormone receptor protein is remarkably reduced according to the treatment concentration of the riboflavin 5'-phosphate sodium treatment group.

The above experimental results demonstrate that riboflavin 5'-phosphate sodium can inhibit the expression of hair follicle degenerative factors.

Example 4. Preparation of external composition containing riboflavin 5'-phosphate sodium

An external composition containing riboflavin 5'-phosphate sodium was prepared, and the following experiment was performed to confirm the efficacy of the composition for preventing hair loss and promoting hair growth. Riboflavin 5'-phosphate sodium used in this example was purchased from Sigma Aldrich.

Example 4-1. Preparation of hair tonic containing riboflavin 5'-phosphate sodium

A hair tonic including the ingredients shown in Table 5 below was prepared. A hair tonic containing riboflavin 5'-phosphate sodium in 0.01, 0.1 and 0.2 wt% was prepared and named as Preparation Examples 1 to 3, respectively. Comparative examples include those that do not contain riboflavin 5'-phosphate sodium, and Riboflavin 5 Those containing minoxidil instead of sodium '-phosphate and those containing riboflavin instead of sodium riboflavin 5'-phosphate were prepared. In each of the Preparation Examples and Comparative Examples, fragrances and dyes were added in appropriate amounts commonly used for hair tonic, and the included purified water was blended by the remaining amount excluding the ingredients including fragrances and dyes to give the total hair tonic composition 100 weight ratio. made to achieve

Ingredients Weight ratio (%) Preparation Example 1 Preparation 2 Preparation 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 ethanol 55 55 55 55 55 55 55 55 castor oil 5 5 5 5 5 5 5 5 glycerin 3 3 3 3 3 3 3 3 Riboflavin 5'-phosphate sodium 0.05 0.1 0.5 0 0 0 0 0 minoxidil 0 0 0 0 One 0 0 0 Riboflavin 0 0 0 0 0 0.05 0.1 0.5 fragrances and colors appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Purified water remaining amount

Example 4-2. Preparation of hair lotion containing riboflavin 5'-phosphate sodium

A hair lotion containing the ingredients as shown in Table 6 was prepared. Flavors and dyes were added in appropriate amounts commonly used in hair lotions, and the included purified water was blended by the remaining amount excluding the ingredients including fragrances and dyes to achieve 100 weight ratio of the total hair lotion composition.

Ingredients Weight ratio (%) cetostearyl alcohol 2 Stearyltriethylammonium chloride 2 Hydroxyethyl Cellulose 0.5 Riboflavin 5'-phosphate sodium 0.01 fragrances and colors appropriate amount Purified water remaining amount

Example 4-3. Hair growth promoting effect of hair tonic containing riboflavin 5'-phosphate sodium

In order to confirm the hair growth promoting effect using the hair tonic compositions according to Preparation Examples and Comparative Examples prepared in Example 4-1, the following experiment was performed.

Hair according to Preparation Examples 1 to 3 and Comparative Examples 1 to 5, divided into 8 groups for a total of 64 males and females with significantly fewer hairs than normal, showing symptoms of hair loss, or showing weak symptoms due to thin hair The tonic composition was applied to the hair and scalp 5 times a week for a total of 6 months, and the results were confirmed.

After 6 months of use, the degree of improvement (very good: +3, improved: +2, slightly improved: +1, no change: 0, slightly worse) in terms of hair thickness, hair density, hair elasticity, and overall evaluation items : -1, bad: -2, very bad: -3) was evaluated by asking the questionnaire and expressing the average value, and the results are shown in Table 7 below.

sample thickness of hair density of hair elasticity of hair Overall rating Preparation Example 1 2.3±0.3 2.4±0.1 2.2±0.2 2.3±0.1 Preparation 2 2.4±0.1 2.6±0.2 2.4±0.4 2.5±0.2 Preparation 3 2.7±0.1 2.9±0.3 2.7±0.2 2.8±0.1 Comparative Example 1 -0.4±0.1 0.1±0.3 0.1±0.2 0.1±0.2 Comparative Example 2 2.2±0.1 2.1±0.4 2.0±0.2 2.1±0.3 Comparative Example 3 2.0±0.2 1.9±0.2 2.1±0.2 2.0±0.2 Comparative Example 4 2.1±0.4 2.0±0.2 2.2±0.3 2.1±0.3 Comparative Example 5 2.2±0.4 2.1±0.2 2.3±0.3 2.2±0.3

As can be seen in Table 7, in the case of the hair tonic of the preparation according to the present invention, superior hair thickness, hair density, and hair elasticity were achieved compared to each comparative example. In particular, in the case of Preparation Example 2 according to the present invention, it was determined to be superior to the hair tonic (Comparative Example 2) containing the same amount of minoxidil, and in the case of Preparation Example 3, the hair tonic containing the same amount of riboflavin (Comparative Example) 3, 4, and 5) were confirmed to be superior.

The experimental result is to prove the hair growth promoting efficacy of the composition for external use containing riboflavin 5'-phosphate sodium as an active ingredient.

It goes without saying that the present invention is not limited to the embodiments described above, and a combination of the embodiments or a combination of at least one of the embodiments and a known technology may be included as another embodiment.

Although the present invention has been described in detail through specific examples, this is for the purpose of describing the present invention in detail, and the present invention is not limited thereto. It will be clear that the transformation or improvement is possible.

All simple modifications and variations of the present invention fall within the scope of the present invention, and the specific scope of protection of the present invention will be made clear by the appended claims.

Claims (7)

As an external composition for preventing hair loss or promoting hair growth, comprising riboflavin 5'-phosphate or an acceptable salt thereof as an active ingredient,
The composition is
The composition for topical application that does not contain riboflavin, riboflavin derivatives or salts thereof other than the riboflavin 5'-phosphate or an acceptable salt thereof. According to claim 1,
The riboflavin 5'-phosphate or an acceptable salt thereof is an external composition for preventing hair loss or promoting hair growth, characterized in that it inhibits male hormone activity. According to claim 1,
The riboflavin 5'-phosphate or an acceptable salt thereof is an external composition for preventing hair loss or promoting hair growth, characterized in that it inhibits male hormone receptor protein expression. According to claim 1,
The riboflavin 5'-phosphate or an acceptable salt thereof is an external composition for preventing hair loss or promoting hair growth, characterized in that it inhibits the expression of a hair follicle degenerative factor. 5. The method of claim 4,
The hair follicle degenerative factor is an external composition for preventing hair loss or promoting hair growth, characterized in that at least one selected from the group consisting of Dkk-1, TGFb-1 and EGF. According to claim 1,
The riboflavin 5'-phosphate or an acceptable salt thereof is an external composition for preventing hair loss or promoting hair growth, characterized in that it is contained in an amount of 0.0001 to 50% by weight based on the total weight of the composition. 7. The method according to any one of claims 1 to 6,
The composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, hair aerosol, Hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair dye, wave agent for hair, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, hair A composition for external application in the form of a mousse or hairspray.

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