KR102226201B1 - Method for enhancing growth of crops by sound wave - Google Patents

Abstract

The present invention relates to a method for promoting the growth of crops using sound waves, and more particularly, to a method for promoting the growth of crops using complex sound waves obtained by first and second treatment of single sound waves in a specific sound range.
According to the present invention, it is possible to shorten the growth period of crops and increase the amount of production at the same time, and by reducing the use of chemical fertilizers, it is possible to conserve farm income and implement environment-friendly agriculture.

Description

Method for enhancing growth of crops by sound wave}

The present invention relates to a method for accelerating the growth of crops using sound waves, and more particularly, to a method for promoting the growth of crops using complex sound waves obtained by first and second treatment of single sound waves in a specific sound range.

The Food and Agriculture Organization of the United Nations (FAO) predicts that the world's population will reach about 9 billion by 2050. Food production is expected to provide a stable supply of food to a large growing population. It is predicted that it should increase by about 70% from the present. Until now, chemical fertilizers and synthetic pesticides have been widely used to shorten the growth period of crops and increase production, and problems such as environmental pollution have been pointed out. In particular, the economic loss due to salt disturbance due to excessive use of chemical fertilizers and pesticides in the facility cultivation area is very large. In addition, while the agricultural production cost is increasing due to excessive nitrogen fertilizer input, there is a side effect of reducing the production amount of crops and resistance to pests.

On the other hand, research to improve the growth and vitality of plants using sound waves has been attempted since Charles Darwin, who insisted on the theory of evolution around 1860, and studies on the method of suppressing the occurrence of plant diseases and pests using sound waves have also recently been attempted. Has become. As a known technique in which sound waves are applied to plants, Korean Patent Registration No. 10-0027825 discloses a method of increasing the growth rate of plants by applying sound waves while treating plants with an aqueous solution of gibberellin. The issue discloses a method for promoting plant growth using music with a frequency of 2000 Hz or less. In addition, Korean Patent Registration No. 10-0325311 discloses a method of suppressing the occurrence of pests by playing sound waves in a frequency range of 2 to 20 kHz to a target crop.

The Korean Patent Registration No. 10-0027825 discloses a method of increasing the growth rate of plants by applying sound waves, but it is a method of applying sound waves while processing the growth promoting composition gibberellin. There is a disadvantage of not being able to do so, and the Korean Patent Registration No. 10-0131133 discloses a method for promoting plant growth using sound waves, but the main band is reduced to a frequency of 2,000 Hz or less by harmonizing the sound recorded in nature with the playing sound using a musical instrument. As a way of listening to music possessed, there is a hassle of having to harmonize the sound recorded in nature with the sound played by the instrument.

Experiments related to plant growth enhancement using sound waves have been carried out for a long time, but it is difficult to ascertain appropriate conditions such as effective pitch range and treatment time, so a method of specifically enhancing crop growth using sound waves has not been attempted.

Under this background, the present inventors seek to solve problems such as environmental pollution caused by chemical fertilizers and synthetic pesticides that are excessively used to promote the growth of crops, and at the same time develop a technology to promote the growth of crops in an environment-friendly manner. As a result of diligence, the elongation and fresh weight of the roots increased by the complex sound waves in which each single sound wave of a specific pitch range was processed in the first and second stages on the seeds and seedlings of Arabidopsis, a model plant, and the period of formation of flower stalks during seedling growth By confirming the effect of promoting growth such as speeding up, the method of promoting crop growth using the composite sound wave of the present invention was completed.

Korean Patent Registration No. 10-0027825 Korean Patent Registration No. 10-0131133 Korean Patent Registration No. 10-0325311

An object of the present invention is to provide a method for promoting the growth of crops using a composite sound wave.

In order to achieve the above object, the present invention comprises the steps of: a) first processing a single sound wave selected from a sound range of 6 to 9 kHz in a plant body; And b) secondary treatment of a single sound wave selected from a frequency range of 9 to 13 kHz on the plant in which the single sound wave of the step a) is first treated.

According to the present invention, it is possible to shorten the growth period of crops and increase the amount of production at the same time, and by reducing the use of chemical fertilizers, it is possible to conserve farm income and implement environment-friendly agriculture.

FIG. 1 is a photograph showing the degree of root growth of Arabidopsis seedlings for each sound region after 5 days of treatment with a composite sound wave (a first sound wave of 9 kHz, a second sound wave of 9 kHz, 10 kHz, and 13 kHz) according to the present invention (A ), and a graph (B) showing the result of comparing the root length by quantifying it.
Figure 2 is a photograph (A) confirming the degree of root elongation of Arabidopsis seedlings for each sound region after 7 days of treatment with a composite sound wave (a first sound wave of 9 kHz, a second sound wave of 9 kHz, 10 kHz, and 13 kHz) according to the present invention. It is a graph (B) showing the result of comparing the root length by quantification.
FIG. 3 is a photograph showing the degree of root elongation of Arabidopsis seedlings for each sound region after 7 days of processing the composite sound wave (first order sound wave of 9 kHz, 6 kHz, 7 kHz, 8 kHz, 11 kHz, and 12 kHz) according to the present invention (A ), and a graph (B) showing the result of comparing the root length by quantifying it.
4 is a photomicrograph of a root apical part of Arabidopsis thaliana after 10 days of treatment with a compound sound wave (a first sound wave of 9 kHz, a second sound wave of 13 kHz) according to the present invention.
Figure 5 is a photograph (A) and quantification of the degree of root elongation of Arabidopsis seedlings after 10 days of treatment with a compound sound wave (first sound wave of 9 kHz, second sound wave of 9 kHz, 10 kHz, and 13 kHz) according to the present invention. Thus, a graph (B) showing the result of comparing the root length, and a graph (C) showing the result of comparing the root live weight by tone range of the Arabidopsis seedling.
6 is a photograph (A) confirming the degree of root elongation of Arabidopsis thaliana seedlings after 10 days of single sound wave (9 kHz, 10 kHz, and 13 kHz) treatment according to the present invention, and a graph showing the result of comparing root length by quantifying it ( B).
7 is a photograph showing a comparison and confirmation of the growth degree of each sound region after 21 days of soil transplantation of Arabidopsis thaliana treated with a composite sound wave according to the present invention.
8 is a photograph comparing and confirming the growth degree of each sound region after 25 days of soil transplantation of Arabidopsis thaliana treated with the composite sound wave according to the present invention.
9 is a photograph comparing and confirming the growth degree of each sound range after 32 days of soil transplantation of Arabidopsis thaliana treated with the composite sound wave according to the present invention.

The present invention relates to a method for accelerating the growth of crops using sound waves, and more particularly, to a method for promoting the growth of crops using complex sound waves obtained by first and second treatment of single sound waves in a specific sound range.

In one aspect, the present invention comprises the steps of: a) primary processing a single sound wave selected from a range of frequencies of 6 to 9 kHz on a plant; And b) secondary treatment of a single sound wave selected from a frequency range of 9 to 13 kHz on the plant in which the single sound wave of the step a) is first treated.

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In the present invention, the term "sound wave" refers to a change in the density of air molecules, that is, a change in atmospheric pressure, which is transmitted into the air while giving the same change to adjacent air molecules. By sensing the energy generated by sound waves, you feel the sound. A sound wave is an acoustic wave that propagates through a medium as a wave felt as sound, and the audible frequency of sound waves that can be heard by ordinary people is about 20 to 20,000 Hz. Sound waves of the audible frequency or more, that is, sound waves of 20,000 Hz or more, are called ultrasonics, and sound waves of the audible frequency or less, that is, sound waves of 20 Hz or less, are called infrasonics, and all of these are called sound waves in a broad sense. .

In the present invention, a'complex sound wave' of a method of first processing and second processing'single sound wave' in a specific sound range was used, and the specific sound wave processing was performed by Adoba Audition 30 software (Adobe System Company) program was used to create the desired sound wave, and a specific single sound wave was processed as a compound sound wave on the plant in the first and second order through the speaker mounted in the soundless growth chamber.

Specifically, in the complex sound wave processing, a single sound wave selected from a frequency range of 6 to 9 kHz is first processed on a plant, and a single sound wave selected from a sound range of a frequency of 9 to 13 kHz is secondarily processed on the plant where the single sound wave is first processed. However, it is not limited thereto.

In the complex sound wave processing, the primary processing of the single sound wave may be performed at a frequency of 6 to 9 kHz, 7 to 9 kHz, or 8 to 9 kHz, and may be preferably processed at 9 kHz, but is not limited thereto. .

In the complex sound wave processing, the secondary processing of the single sound wave may be performed at a frequency of 9 to 13 kHz, 10 to 13 kHz, or 11 to 13 kHz, and may be preferably processed at 13 kHz, but is not limited thereto. .

In the complex sound wave treatment, a single sound wave may be treated on the plant at a volume of 50 to 100 decibels (dB) for 1 to 3 hours per 24 hours, and preferably can be treated on the plant at a volume of 80 decibels for 2 hours per 24 hours. However, it is not limited thereto.

In addition, in the complex sound wave treatment, the first and second treatment of a single sound wave to the plant may be treated for 1 to 6 days, preferably for 4 to 6 days, but is not limited thereto.

In the complex sound wave treatment, a single sound wave may be applied to seeds or seedlings of a plant, but is not limited thereto.

The term "plant" of the present invention refers to a body of a type possessed by a plant, and may collectively be used interchangeably with'plant' or'crop', and may include the whole plant, part of the plant, seeds or plant cells. .

The plant is not limited thereto, but food crops including rice, wheat, barley, corn, beans, potatoes, red beans, oats and sorghum; Vegetable crops including Arabidopsis daikon, Chinese cabbage, radish, pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, pumpkin, green onion, onion and carrot; Specialty crops including ginseng, tobacco, cotton, sesame, sugar cane, sugar beet, perilla, peanut and rapeseed; Fruit trees including apple tree, pear tree, jujube tree, peach, parsley, grapes, tangerines, persimmons, plums, apricots and bananas; Flowers including roses, gladiolus, gerberas, carnations, chrysanthemums, lilies and tulips; And it may be selected from the group consisting of feed crops including ryegrass, red clover, orchardgrass, alpha alpha, tolpescue, and perennial ryegrass.

In the present invention, "promoting the growth of crops" is not limited thereto, but promoting the germination of plant seeds, promoting the growth of rhizomes of plants, promoting the growth of the length of the plant, promoting the growth of the bulk, promoting the fruiting of the fruit, promoting the ripening of the fruit, It is the most comprehensive concept that includes all of the enhancement of leaf activity, promotion of plant root growth, promotion of plant growth, and promotion of fruit coloring.

In the present invention, the above-ground growth of plants may be promoted by the composite sound wave. The above-ground part of the plant may refer to all areas where water is exposed to the outside of the soil, and includes all of leaves, stems, roots, flowers, fruits, and the like.

In one embodiment of the present invention, a single sound wave of 9 kHz is first treated for 5 days for 5 days for 2 hours per day on the seeds and seedlings of Arabidopsis as a model plant, and the single sound waves are applied to the seeds and seedlings of Arabidopsis As a result of analyzing the growth state of Arabidopsis by processing a single sound wave of 9 kHz, 10 kHz, 11 kHz, 12 kHz and 13 kHz for 5 or 7 days for 2 hours per day, that is, complex sound waves, the result of analyzing the growth state of Arabidopsis thaliana, the sound wave untreated group, That is, it was confirmed that the growth of the root was promoted by increasing the elongation and fresh weight of the root compared to the control group (FIGS. 1 to 5).

In an embodiment of the present invention, the complex sound wave treatment under the same conditions as described above (primary treatment: 9 kHz single sound wave, 2 hours per day for 5 days; Secondary treatment: 9 kHz, 10 kHz and 13 kHz single sound wave, per day As a result of analyzing the growth state of the above-ground part by transplanting the Arabidopsis seedlings completed (2 hours each, 5 days) into the soil, the Arabidopsis plant is taller than the control group (no sound wave treatment group), the leaves are very rich, and the flower stalk is formed. It was confirmed that the growth of the above-ground part of the plant was promoted, such as speeding up the timing (FIGS. 7 to 9).

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

Example 1: Preparation of publicly available crops

Arabidopsis thaliana seeds (ecotype Col-0) were sold and used at the Arabidopsis Biological Resource Center (Ohio State Univ., USA).

Example 2: sound wave processing and Growth Prize Analysis of the growth status of Arabidopsis thaliana within

A plastic Petri dish containing 5g of Arabidopsis seeds was properly placed in a soundless growth chamber (manufactured by Korea Science and Technology Industry Co., Ltd.) and then treated with sound waves. The environmental conditions in the growth bed were a long day treatment condition controlled by 16 hours light treatment and 8 hours dark treatment, and the temperature was 23±1°C, and the humidity was maintained at 50-80%.

Specifically, the first sonic treatment was performed on seeds, and 9 kHz sonic waves were treated for 5 days at 2 hours a day (2 hours/day), and then vernalization was performed at 10° C. for 2 days. Seeds that had been subjected to anabolic treatment were surface sterilized and placed on MS medium. Twenty seeds per petri dish were sown in a row and subjected to secondary sonication. The sound wave treatment was untreated, 6kHz, 7kHz, 8kHz, 9kHz, 10kHz, 11kHz, 12kHz, and 13kHz total 8 kinds of sound waves were treated for 2 hours a day (2 hours/day) for 5 days. At this time, the loudness (decibel, dB) was fixed at 80dB.

The seed was treated with 9 kHz sound waves first, and then the total of eight types of sound waves were treated secondly to the seed that was sown, that is, the growth status of Arabidopsis seedlings completed with complex sound wave treatment was grown on the growth bed, and the growth state was confirmed, taking a photo. And observed. This experiment was repeated four times.

Example 3: Verification of the effect of promoting root growth of Arabidopsis thaliana by complex sound wave treatment

In order to select the optimal range for promoting the growth of crops, 9 kHz sound waves were first processed on the seeds and seedlings of Arabidopsis, a model plant, and a total of 6 kHz, 7 kHz, 8 kHz, 9 kHz, 10 kHz, 11 kHz, 12 kHz, and 13 kHz. Each of the eight types of sound waves was treated as a second order, that is, the degree of acceleration of root growth of Arabidopsis thaliana was confirmed by processing a compound sound wave in which single sound waves having different sound bands were mixed.

Specifically, the sound wave processing was performed in a noiseless growth bed in which a speaker was installed according to the sound wave processing method of Example 2, and the primary sound wave treatment of 9 kHz and the total eight types of sound waves were processed and processed for 2 hours a day. After completion, it proceeded to the process of growing in the growth bed without sound waves. At this time, all of the experimental groups were subjected to sound wave treatment and growing in the same growth bed.

As a result of examining the degree of growth after 5 and 7 days of the roots of Arabidopsis thaliana having completed the first and second sonic treatment, that is, complex sonic treatment, as shown in Figs. 1 and 2, the sonic non-treatment group, That is, it was confirmed that the root elongation was increased in the experimental group treated with the secondary sound waves of 9 kHz, 10 kHz and 13 kHz compared to the control group. Specifically, compared to the untreated group, the elongation of the roots after 5 and 7 days was 13% and 17% respectively at 9 kHz treatment, 32% and 27% at 10 kHz treatment, and 27% and 24% at 13 kHz treatment, respectively. It was confirmed that it had grown.

In addition, as shown in Figure 3, as in the experimental group treated with the secondary sound waves of 9 kHz, 10 kHz and 13 kHz, the experimental group treated with the secondary sound waves of 11 kHz and 12 kHz was also confirmed that the root elongation was increased compared to the control (control). In contrast, in the case of the experimental group treated with the secondary sound waves of 6 kHz, 7 kHz, and 8 kHz, it was confirmed that there was no significant difference in root elongation compared to the control group.

Through the above results, it was found that the roots of Arabidopsis thaliana, which processed sound waves of 9 kHz or more as secondary sound waves, were observed compared to the non-sound wave treatment group. Accordingly, in order to confirm the difference in the roots treated with the complex sound waves in the first and second phases from the untreated group, the root tip was observed with a dissecting microscope.

As shown in FIG. 4, in the case of the sonic non-treatment group, it was confirmed that the roots grew slightly sparse, and the root apex was also observed to be shorter than the roots treated with the complex sound wave. On the other hand, the apical end of the Arabidopsis root treated with the complex sound wave (first sound wave; 9 kHz, second sound wave; 13 kHz) for 10 days (the part marked with bar in Fig. 4) was longer than that of the untreated group, and overall root hair It was confirmed that the number of is also large, and that it grows well densely. In other words, it was confirmed that the growth of Arabidopsis seedling roots was promoted by the complex sonic treatment.

Through the above results, it was found that the complex sonic treatment has an effect on the growth of the root, which can facilitate the absorption of nutrients contained in the medium during the growth of the crop, and ultimately induce the growth of the crop. I could see that there was.

Thereafter, in order to re-verify the above results, the amount of seeds of Arabidopsis was increased and the degree of root growth of Arabidopsis was reconfirmed. At this time, the sound waves were processed under conditions of 9 kHz, 10 kHz, 11 kHz, 12 kHz, and 13 kHz, which are the sound bands of the longer root length, as described above, and the conditions of the 6 kHz, 7 kHz and 8 kHz sound bands where there was no difference in the height of Arabidopsis roots were excluded. The 6kHz, 7kHz, and 8kHz sound bands, which had insignificant growth promotion effects, were excluded by repeating the experiment several times, and the process of selecting the optimal sound range excellent for growth promotion was repeated, and finally, as a secondary sound wave processing condition, 9kHz, 10 kHz and 13 kHz conditions were established, and re-verified focusing on the above conditions.

As a result of germinating the roots of Arabidopsis seedlings that have been treated with complex sound waves according to the optimal secondary sound wave condition, and checking the root growth degree and live weight 10 days after germination, as shown in FIG. 5, the sound wave untreated group, That is, it was confirmed that the height and fresh weight of the roots were increased in each experimental group treated with complex sound waves under the conditions of 9 kHz, 10 kHz and 13 kHz compared to the control group. Specifically, it was confirmed that the elongation and fresh weight of the roots increased by 20% and 15% respectively at 9 kHz treatment, 22% and 17% at 10 kHz treatment, and 28% and 21% at 13 kHz treatment compared to the untreated group. , It was found that the root elongation and fresh weight increase were the best when treated at 13 kHz.

On the other hand, in order to re-verify whether the root growth promoting effect of Arabidopsis thaliana according to the first and second complex sound wave treatment, or simply the effect of promoting root growth according to the sound wave treatment, the root elongation and the increase in live weight were confirmed at 9 kHz, 10 kHz And the first sound wave treatment, that is, the single sound wave was processed under the condition of 13 kHz to check the degree of root growth of Arabidopsis thaliana.

As a result, as shown in Fig. 6, root growth is increased compared to the untreated group even when single sound wave treatment is performed, but in the case of the experimental group (Fig. 5) treated with complex sound waves in the first and second steps It was confirmed that the promoting effect was more excellent.

That is, compared to the single sound wave treatment, it was found that when the complex sound waves were processed over the first and second orders, the root growth of Arabidopsis seedlings was more excellently promoted.

Example 4: Analysis of growth status of Arabidopsis thaliana in soil

In Example 3 above, it was confirmed that the root length and fresh weight of the Arabidopsis seedlings completed with the complex sonication treatment were increased compared to the untreated group at the initial stage after germination. Therefore, to analyze the relationship between whether the phenomenon that the root growth was good induces the results of promoting Arabidopsis growth, Arabidopsis seedlings grown up to 10 days after germination on the growth bed after completion of sonic treatment were transplanted into a pot with soil. While growing until after 1 day, the growth state of the above-ground part was confirmed. This experiment was repeated four times.

Example 5: sonicated Arabidopsis thaliana Seedling Verification of the effect of promoting growth in soil

Based on the above Examples 2 and 3, the growth state after 21, 25, and 32 days while transplanting Arabidopsis thaliana, whose root growth was improved as a result of complex sonic treatment in MS medium, into a pot with soil and growing in a greenhouse. Was confirmed.

As a result, it was confirmed that after 21 days, the width of the leaves of Arabidopsis thaliana treated with the composite sound wave was much wider, the overall height was long, and part of the flower stalk was formed compared to the control group (control; non-sound treatment group) (FIG. 7). In addition, it was confirmed that the same pattern as after 21 days was maintained even after 25 days, among which Arabidopsis was treated with 10 kHz as the secondary sound wave condition, and flower stalk formation proceeded faster than the control group and the experimental group in the 9 kHz and 13 kHz sound band conditions. It was confirmed that it is becoming. On the other hand, it was confirmed that the leaves of Arabidopsis thaliana treated with a sound wave of 13 kHz as a secondary sound wave condition were very rich and relatively large in size (FIG. 8). After 32 days, it was confirmed that the height of the Arabidopsis plant was the largest in the experimental group treated with 10 kHz and 13 kHz as the secondary sound wave conditions, and the leaves were very rich and the flower stalk formation was also good. On the other hand, the experimental group treated with 9 kHz had insignificant difference from the untreated group (FIG. 9).

The growth state of Arabidopsis thaliana after sonication was different according to the sound range of the treated complex sound waves, and 10 kHz as the secondary sound wave condition showed the best tendency until 25 days after transplanting into soil, but rather 13 kHz after 32 days. It was confirmed that the height of the experimental group treated with the secondary sound wave was larger.

In summary, when the Arabidopsis seeds were treated with 9 kHz as the first single sound wave condition and then treated with the second single sound waves of 10 kHz and 13 kHz after sowing, the growth state of the above-ground part was good, and the time of formation of the flower stalk was also It was found that the growth promotion phenomena such as rapid growth were most pronounced.

On the other hand, through the series of results, it can be inferred that it is possible to artificially control gene expression related to physiology and growth metabolism of crops by using sound waves in a specific sound range, and the selected sound waves in a specific sound range according to the present invention. It can be seen that the treatment can induce the regulation of the expression of related genes, and finally promote the growth of the crop through the control of the growth of the crop.

Claims (5)

a) first processing a single sound wave selected from a frequency range of 6 to 9 kHz to the seeds of the plant;
b) a step of annealing the seeds of the plant that has been subjected to the primary treatment of the single sound waves of step a); And
c) secondary treatment of a single sound wave selected from a frequency range of 9 to 13 kHz on the seeds of the plant, which has been subjected to the pornography process in step b),
A method for promoting the growth of crops using complex sound waves, wherein the single sound waves are treated on the plants at a volume of 50 to 100 decibels (dB) for 1 to 3 hours per 24 hours.
delete The method of claim 1, wherein the plant is treated with a single sound wave for 1 to 6 days.
delete According to claim 1, wherein the plant is a food crop including rice, wheat, barley, corn, beans, potatoes, red beans, oats and sorghum; Vegetable crops including Arabidopsis daikon, Chinese cabbage, radish, pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, pumpkin, green onion, onion and carrot; Specialty crops including ginseng, tobacco, cotton, sesame, sugar cane, sugar beet, perilla, peanut and rapeseed; Fruit trees including apple tree, pear tree, jujube tree, peach, parsley, grapes, tangerines, persimmons, plums, apricots and bananas; Flowers including roses, gladiolus, gerberas, carnations, chrysanthemums, lilies and tulips; And ryegrass, red clover, orchardgrass, alpha alpha, tall fescue and perennial ryegrass.

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