KR101879625B1 - Cosmetic Composition for Improving Atopic Dermatitis Comprising Extract of Tagetes Minuta - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving atopic dermatitis, which is characterized by containing an extract of Aspergillus oryzae as an active ingredient.
The cosmetic composition for improving atopic dermatitis according to the present invention exhibits an anti-allergic, antioxidant and anti-inflammatory effect and thus has the effect of improving atopic dermatitis by containing the extract of Radix guinea pigs as an active ingredient.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving atopic dermatitis,

The present invention relates to a cosmetic composition for improving atopic dermatitis, which contains an extract of Radix japonicum.

Atopy occurs mainly in infancy and is called as fever, and it occurs mainly as the content of ceramides in the stratum corneum is decreased.

The cause of atopic dermatitis has not been clarified yet, but it is presumed that it is caused by the disturbance of the immune system.

In general, atopic dermatitis is dry due to a decrease in the water retention function of the stratum corneum, and skin barrier function is lowered, so that skin allergy can easily occur, so that it is prone to be pruritic, repeatedly recurring, do.

In many cases, it is exacerbated repeatedly at intervals of 1 to 2 weeks. If not properly managed, it progresses to subacute lesions of erythematous eruption with exudation, chronic lesions of thickening skin like skin.

The cause of atopic skin is divided into two main causes. One is genetic cause, and it is inherently deficient in gamma-linoleic acid (GLA) This is caused by the weak and easily disappearing of moisture on the surface of the skin. Other causes are environmental factors, such as dry living environment, reactive oxygen species, bacteria, viruses, fungi, etc. [Dermatology, Korean Society of Dermatology Editorial Committee,

Atopic dermatitis causes mast cells to release an allergic reaction inducer such as histamine in the intracellular granules, indicating degranulation [Gao ZG, et al., Biochem Pharmacol. 2009. Nov 5]. In addition, atopic dermatitis has been reported to be associated with allergic rhinitis and asthma, and thus it is highly likely to be an allergic disease [de Meer G, et al., Pediatr Allergy Immunol. 2009. Nov 13].

A composition comprising as a main ingredient various natural substances for improving atopic dermatitis has been disclosed. In Korean Patent Publication No. 10-1418916, a composition for improving atopic dermatitis comprising an extract of Chrysantida or an active fraction isolated therefrom by chromatography as an active ingredient is disclosed Korean Patent Publication No. 10-1382115 discloses a food composition containing ginseng fruit extract as an active ingredient and improving atopic dermatitis or dryness. Korean Patent Publication No. 10-1083347 discloses a food composition containing a moss extract as an active ingredient A pharmaceutical composition for improving or treating atopic dermatitis is disclosed.

On the other hand, Tagetes minuta (Tagetes minuta) is an annual plant of Asteraceae called garbage grass and widely distributed in the wild.

In Korea, some plants are grown for the purpose of ornamental use, but in foreign countries, they have been used for fragrance, coloring and medicinal resources in addition to ornamental use. It is commercially grown in South America, India and southern Europe.

For example, in southern India, Europe, South America, and the United States, private prescriptions are used to control the infusion of raw water from aquatic plants. It has been shown to be effective for the treatment of liver disorders, intestinal diseases, diarrhea, abdominal pain, headache, pneumonia, cough and fungal skin diseases as well as anticonvulsant, anti-inflammatory, antibacterial and antimicrobial effects [Radzen et al , ≪ / RTI > Parfmer und Kosmetik, 67, 52-55 (1986); Zygadlo et al., Lag. J. Essent. Oil Res., 6, 617-621 (1994); Singh et al., Indian Perfumer, 39, 102-106 (1995); Bicchi et al., Flavor Fragr. J., 12, 47-52 (1997)).

As an example of utilizing such an abalone, Korean Patent Registration No. 10-0979059 merely serves as a raw material of a dye for natural dyeing. In other words, there has not yet been reported an example in which a composition for improvement of atopic dermatitis is used as a raw material for aquatic plants.

The inventors of the present invention have confirmed the efficacy for improving atopic dermatitis when using an extract of a natural product, Mansuo japonica as an active ingredient, and studied a composition containing the same to complete the present invention.

Korean Patent Publication No. 10-1418916 Korean Patent Publication No. 10-1382115 Korean Patent Publication No. 10-1083347 Korean Patent Publication No. 10-0979059

It is an object of the present invention to obtain an effect of improving atopic dermatitis by containing an extract of Radix Tamiflu as an active ingredient.

In order to achieve the above object, the cosmetic composition for improving atopic dermatitis according to the present invention is characterized in that it contains an extract of Aspergillus oryzae as an active ingredient.

At this time, the water extract of Suifuji radish can be an extract obtained by adding a water-soluble salt of C1-C4 or a mixture of water and C1-C4 alcohol and extracting it, preferably by adding it to methanol.

The cosmetic composition for improving atopic dermatitis according to the present invention is characterized in that it contains 0.001 to 97% by weight, preferably 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The cosmetic composition for improving atopic dermatitis according to the present invention as described above exhibits an antiallergic, antioxidant, anti-inflammatory effect and thus has the effect of improving atopic dermatitis by containing the extract of Radix guinea pigs as an active ingredient.

FIG. 1 is a graph showing the results of analysis of inhibition of secretion amount of? -Hexosaminidase according to the concentration of the extract of Radix Salviae Radix extract of the present invention.
FIG. 2 is a graph showing the results of analysis of the ABTS radical scavenging ability according to the concentration of the extract of Radix Salviae Radix extract of the present invention.
FIG. 3 is a graph showing the results of analysis of DPPH radical scavenging ability according to the concentration of the extract of Radix Salviae Radix extract of the present invention.
FIG. 4 is a graph showing the results of analysis of inhibitory effect on NO production according to the concentration of the extract of Radix Salviae Radix extract of the present invention. FIG.
FIG. 5 is a graph showing the relative content of positive control group according to the concentration of the water extract of the present invention.

As used herein, "atopic dermatitis" is defined as including any disease classified as atopic dermatitis in the art, regardless of its direct or indirect cause.

In general, atopic dermatitis is classified into infantile atopic dermatitis, infantile atopic dermatitis, adult atopic dermatitis, and maternal atopic dermatitis according to the onset period or the object of the invention. In this specification, atopic dermatitis includes all types of atopic dermatitis .

As shown in the following experimental examples of the present invention, clinical tests were conducted on 100 subjects (total 4 groups) having randomly selected atopic dermatitis symptoms, and as a result, it was found that about 85 to 95% Respectively.

These results are obtained for randomly selected subjects. Thus, the composition for improving atopic dermatitis of the present invention may be effective for all types of atopic dermatitis regardless of the cause of atopic dermatitis.

The term "improvement" as used in the present invention means all actions in which the symptoms of atopic dermatitis are alleviated or changed by administration of the composition of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is characterized by containing an extract of Fusarium oxysporum as an active ingredient.

The above-mentioned water extract of Fertility Supernatant may be an extract obtained by adding C1-C4 alcohol or a mixture of water and C1-C4 alcohol to dried Fertilizer. The C1 to C4 alcohols may be any one or more of methanol, ethanol, propanol, isopropanol, and butanol. The alcohol may be used as a mixed solvent mixed with water.

In one preferred embodiment of the present invention, methanol is used for the extraction.

To be more specific, the above-mentioned Extracts of the Extra Virgin Rice are cut into 2 to 3 cm and used by using a cutting machine. The cut pieces of the mulberry leaves are dried at a temperature of 30 to 50 ° C. for 2 to 4 days, and 75 to 85% by weight of methanol is added to the dry weight of 400 to 600 g, and extracted with ultrasonic equipment and filtered.

The extract of Radix Salvia Radix extract of the present invention is used as an active ingredient of a cosmetic composition for improving skin wrinkles, wherein the Radix sativus extract may be contained in an amount of 0.001 to 97% by weight, preferably 0.01 to 20% %, ≪ / RTI > but is not limited thereto.

In the case of a cosmetic composition, the cosmetic composition may be prepared by using the above-mentioned extract of Radix Seed sauvignon as an active ingredient, and for example, a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, , Microgranules or ionic forms (liposomes), non-ionic follicular dispersions, creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The cosmetic composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, , Ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics And may contain adjuvants conventionally used in the cosmetics field, such as any other ingredient.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by Examples and Experimental Examples.

<Examples>

* Extracts of Mulgariae japonica were prepared as follows.

All solvents used were analytical grade, purchased from Sigma-Aldrich (USA) or Merck (USA).

The outpasted shoots were cut into 2.54 ㎝ by using a round bar, and then dried in a drying oven at 40 캜 for 3 days. The outgrowths of about 500 g plant weight were extracted with 80 wt% methanol using an ultrasonic device. These liquids were filtered through a membrane filter (0.2 μm), and the solvent was removed in a vacuum to leave a methanol extract. Through this process, the cellulose of the components of the water ginseng was filtered through the purification process, and the water extract of the ginseng extract was a methanol extract mainly containing trace components of the water ginseng.

<Experimental Example 1> Evaluation of anti-allergy effectiveness

Anti-allergic efficacy was assessed by measuring the amount of β-hexosaminidase. The inhibition of β-hexosaminidase secretion is an index indicating the degree of prevention of deaggregation of immune cells, and the results of the analysis are shown in FIG.

The experiment was carried out as follows.

First, Rat basophlic leukemia cell line in RBL-2H3 cell line 15% FBS, 100 unit / ml of penicillin and streptomycin MEM medium containing hygromycin and 37 ℃, 2 ㅧ 10 ⁵ cells on a 24 well plate in 5% CO ₂ conditions / well and cultured for 24 hours. After removal of the supernatant, MEM medium containing 25 ng / ml anti-DNP IgE was added, incubated for 4 hours, washed twice with PIPES buffer, and PIPES buffer containing 4 mM MgCl ₂ , 5.6 mM glucose and 0.1% Min.

Next, the samples were treated with 25, 50, and 100 μg / ml of 20 ng / ml of DNP-BSA and reacted for 20 min. After the reaction was terminated by ice for 10 minutes, the supernatant was added to a 96-well plate, and 1 mM P-nitrophenyl-acetyl-β-D-glucosaminyl was added thereto and reacted at 37 ° C. for 1 hour. Next, stop solution (0.1 M NaHCO ₃ , 0.1 M Na ₂ CO ₃ ) was added, and the absorbance was measured with an ELISA reader at 405 nm wavelength band. Ketotifen (100 ㎍ / ml) was used as a positive control.

The 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 1.

IC ₅₀ ([mu] g / ml) Extracts 92.79 Ketotifen 38.97

As a result of the cytotoxicity evaluation, it was confirmed that the extract of Mulberry Soup of the present invention had much higher cell survival rate than the positive control and thus the cytotoxicity was low.

1, it was confirmed that the effect of inhibiting degranulation is dependent on the concentration of the extract of Radix guinea pigs of the present invention. These results demonstrate the antiallergic effect of the extract of Radix Salviae Radix extract of the present invention and can be expected to have the effect of improving atopic dermatitis when used as a cosmetic composition.

<Experimental Example 2> Evaluation of antioxidant effectiveness

Antioxidant efficacy was evaluated by measuring ABTS latacity and DPPH radical scavenging ability.

The ABTS radical scavenging ability was evaluated as follows, and the results are shown in Fig.

The reaction mixture was prepared by mixing 7 mM ABTS buffer and 2.45 mM potassium persulfate buffer (K ₂ S ₂ O ₈ ), mixing ABTS: K ₂ S ₂ O ₈ in a volume ratio of 2: 1 and shading for 12 to 16 hours. Next, the stock buffer was diluted so that the OD value measured at 734 nm was 0.70, 0.02, and 100 μl of the extract sample diluted by 100 μl of the buffer was added, followed by reaction at room temperature for 6 minutes. The absorbance was measured at 734 nm and the IC ₅₀ value for each sample was determined and evaluated. BHA, an antioxidant, was used as a positive control.

The 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 2.

IC ₅₀ ([mu] g / ml) Extracts 28.14 BHA 3.37

As a result of the cytotoxicity evaluation, it was confirmed that the extract of Mulberry Soup of the present invention had much higher cell survival rate than the positive control and thus the cytotoxicity was low.

2, the ABTS radical scavenging activity tended to increase with the concentration of the extract of the present invention.

Next, in order to confirm the DPPH radical scavenging ability, the reaction solution containing the DPPH solution and the sample was reacted at room temperature for 30 minutes, and the absorbance was measured at 517 nm using an ELISA reader. The results are shown in FIG.

In addition, the 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 3.

IC ₅₀ ([mu] g / ml) Extracts 222.3 BHA 19.1

As a result of the cytotoxicity evaluation, it was confirmed that the extract of Mulberry Soup of the present invention had much higher cell survival rate than the positive control and thus the cytotoxicity was low.

The results of FIG. 3 indicate that DPPH radical scavenging ability tends to increase with the concentration of the water extract of the present invention.

The results of the ABTS and DPPH radical scavenging activity show the antiallergic effect of the water extract of the present invention and can be expected to have the effect of improving atopic dermatitis when used as a cosmetic composition.

<Experimental Example 3> Evaluation of anti-inflammatory effectiveness

The anti - inflammatory efficacy was induced by NO synthesis in Raw264.7 cell, and the anti - inflammatory effect was evaluated by analyzing the NO assay. The cytotoxicity was evaluated by analyzing the MTT assay.

The NO synthesis of Raw264.7 cell to evaluate anti-inflammatory efficacy was performed as follows.

Mouse macrophages, raw 264.7 cells, were cultured in a 10% FBS DMEM medium at 37 ° C and 5% CO ₂ . Raw 264.7 macrophages were seeded at a density of 4.3 ㅧ 104 cells / well in a 96 well plate and treated with Lipopolysacharide, a NO synthesis mitogen, at a concentration of 0.5 ㎍ / ㎖ for 24 hours.

After incubation for 1 hour and 30 minutes at 37 ° C and 5% CO ₂ , samples were incubated at concentrations of 6.25, 12.5, 25, 50, 100 and 500 μg / ml or 25, 50, 100, 200 and 400 μg / ml , And incubated for 24 hours.

Next, the NO assay was analyzed for anti-inflammatory efficacy as follows.

1M NaNO ₂ was diluted to a standard curve of 0, 1.56 μM, 3.12 μM, 6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM to obtain the reference value (Reference 1). Next, 1% sulfanilamide and 0.1% N- (1-naphtyl) ethylenediamine dihydrochloride reagent were mixed in a volume ratio of 1: 1, and the culture supernatant of the culture supernatant Were mixed at a volume ratio of 1: 1, and the mixture was stored at room temperature for 10 minutes. ELISA was then measured at 540 nm to measure the inhibitory effect of NO production on the control.

Next, MTT assay was analyzed for cytotoxicity as follows.

NO assay The remaining supernatant was removed and 100 μl of serum-free medium containing 10% 5 mg / ml MTT solution was added. And incubated in a 5% CO ₂ incubator at 37 ° C for 2 hours. The medium was removed again and DMSO was added to 100 μl / well. Finally, it was dissolved in a Shaker for 15 minutes and absorbance was measured at 540 nm using an ELISA reader. The positive control group was L-NNMA.

The 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 4.

IC ₅₀ ([mu] g / ml) Extracts 222.3 L-NNMA 19.1

As a result of the cytotoxicity evaluation, it was confirmed that the extract of Mulberry Soup of the present invention had much higher cell survival rate than the positive control and thus the cytotoxicity was low.

In addition, as shown in FIG. 4, it was confirmed that the inhibitory effect on the NO production was increased according to the concentration of the water extract of the present invention. In addition, the results of FIG. 5 show that the relative content of the extract of the present invention to the positive control was constant depending on the concentration of the extract of the present invention. Thus, the anti-inflammatory activity of the extract of the present invention was confirmed.

* As described above, the extract of the present invention has the effect of antiallergic, antioxidant and anti-inflammation. From these results, it was expected that the use of the extract of the present invention as an active ingredient of a cosmetic composition would have the effect of improving atopic dermatitis.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It is possible to carry out various changes in the present invention.

Claims (4)

A composition for anti-allergy comprising extractant of Tagetes minuta as an active ingredient,
The above extract of Extensible Fertility Supernatant is an extract obtained by adding Extensive Supernatant of Fertility Supernatant to methanol, ethanol, water or a mixed solvent thereof,
Wherein the composition for anti-allergy is applicable to improvement of atopic dermatitis.
The method according to claim 1,
Wherein the extract of Fifty Millet of Anguilla japonica is a mixed solvent extract of water and methanol.
The method according to claim 1,
Wherein the Fertilizer Extract is a 80% methanol extract.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a cosmetic composition.

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