KR101263821B1 - Multiway valve and System for extracting regenerative cells using the same - Google Patents

Abstract

The present invention relates to a multi-way valve and a regenerative cell extraction system using the same. The multi-way valve according to the present invention has a plurality of tubes to which the fluid is transported is placed on the upper surface, the support plate is formed in the through hole in the lower portion of the area where the plurality of tubes are disposed, the cover for opening and closing the upper surface of the support plate, A plurality of shutter members inserted into the through-holes of the support plate so as to be opened and closed to open and close the tubes placed on the support plate, and a center part disposed below the support plate to support the shutter member, the central part being rotatably installed, and an annular shape. And a rotation plate having one or more outer ring portions rotatably disposed to surround the central portion and installed in a rotatable manner, wherein an upper surface of the central portion and the outer ring portion is disposed at a first height than the first ground surface and the first ground surface. It is formed of a second ground surface disposed at a lower second height, the shutter member is supported on the first ground surface It protrudes from the through-hole thereby closing the tube case.

Description

Multiway valve and system for extracting regenerative cells using the same

The present invention relates to a multi-way valve and a regenerative cell extraction system using the same, and more particularly, a system for extracting regenerative cells such as stem cells by centrifuging a cell tissue such as adipose tissue and a multi-way used in the system. It is about a valve.

Stem cells are defined as cells that possess clonogenic and self-renewal ability that can differentiate into multiple cell lines under certain conditions. Embryonic stem cells are derived from mammalian embryos at the blastocyst stage and possess the ability to differentiate into almost all cells present in the body, while adult stem cells are stem cells that are present in trace amounts in differentiated tissue after birth. It is a cell with the capacity of a cell.

Adult stem cells offer practical advantages over embryonic stem cells. In other words, adult stem cells, unlike embryonic stem cells, do not cause ethical problems and can be extracted from the patient. They are abundant in supply and inherent in various tissues of the human body. The most available sources of adult stem cells are bone marrow, peripheral blood, umbilical cord / umbilical cord blood and adipose tissue, as confirmed in recent studies. These cells can maintain, yield and replace the final differentiated cells in their own specific tissues as a result of tissue damage due to physiological cell turnover or wounds.

This ability, called cell plasticity, has led to the development of therapeutic applications aimed at regenerating defective tissues for the purpose of restoring the physiology and function of damaged organs. Adult stem cells are distributed not only in hematopoietic cells but also in various tissues, as known for decades, and as recently discovered, can be differentiated into blood vessels, muscles, bones, cartilage, skin, nerves, and the like. These cells are known as mesenchymal stem cells. In addition, platelet concentrates can be used to accelerate wound healing and, consequently, play a role in regenerative medicine that aids in the reconstitution of tissues such as bone, skin or other tissues.

Recently, adipose tissue has been found to contain large amounts of stem cells, progenitor cells and matrix materials suitable for therapeutic applications. Adipose tissue is also a rich source of vascular endothelial cells, which can contribute to tissue regeneration by promoting growth of renal blood vessels and stimulating stem and progenitor cell growth.

Many devices have been developed for collecting cells from various tissues, such as adipose tissue and cord blood, but these devices are not fully automated, as some of the entire process from tissue collection to processing is performed manually. In addition, conventional devices may not be completely sealed, which may cause problems such as contamination of tissues during the entire process from collection of adipose tissue to treatment.

Therefore, the process from collection of tissues to processing of tissues is fully automated by a program in a sealed state, and development of an apparatus capable of improving the purity of regenerated cells collected from tissues is required.

In addition, the conventional devices are provided with a plurality of connecting pipes and storage bags, the valve has to be installed separately for each connection pipe and a number of pumps, the device is complicated and inconvenient to use.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and provides a multi-way valve and a regenerated cell extraction system equipped with the multi-way valve, which can be used in a device in which a plurality of paths should be formed, including a regenerative cell extraction system. have.

The multi-way valve according to the present invention for achieving the above object is a plurality of tubes to which the fluid is transported is placed on the upper surface, the support plate is formed in the through hole in the lower portion of the region where the plurality of tubes are disposed; A cover that opens and closes an upper surface of the support plate; A plurality of shutter members protruding with respect to an upper surface of the support plate and fitted into a through hole of the support plate so as to be able to be lifted and lowered; And a center part disposed below the support plate to support the shutter member, the center part being rotatably installed, and having one or more outer ring parts rotatably formed to sequentially wrap the center part and rotatably installed. And a top surface of the central portion and the outer ring portion formed of a first ground surface disposed at a first height and a second ground surface disposed at a second height lower than the first ground surface, wherein the shutter member is provided with a first surface. When supported on the support surface protrudes with respect to the support plate.

In addition, the regenerated cell extraction system according to the present invention, for separating the tissue (tissue), the sub-container is formed with a space portion is rotated by receiving a rotational force from the outside and the tissue is accommodated, one end is below the sub-container A first unit having a hollow entrance tube inserted into the sub container so as to be disposed; A main container for extracting regenerated cells from the separation object transferred from the first unit, the main container is rotated by receiving a rotational force from the outside and the receiving portion is formed to accommodate the separation object, and one end is disposed below the main container A second unit having a hollow first discharge pipe inserted into the main container so that the other end thereof is disposed outside the main container; A multi-way valve having the above-described configuration, wherein an access pipe of the first unit, a first discharge pipe of the second unit, and a plurality of storage bags are connected to each other; And a pump connected to the multi-way valve.

The regenerative cell extraction system 300 according to the present invention not only automates the whole process, but also can perform all operations with one pump using two multi-way valves, and the system can be simply configured.

The regenerated cell extraction system according to the present invention performs the first stirring, washing and histolysis in the first unit to form an aqueous solution containing stem cells, in the second unit by performing centrifugal separation for the aqueous solution, Stem cell extraction is performed efficiently, and the extraction yield of stem cells is improved.

In addition, the regenerative cell extracting system is hermetically sealed to maintain the airtightness, and external pollutants are blocked by the filter, thereby preventing contamination of the adipose tissue or cells in the process of extracting stem cells from the adipose tissue or washing the tissue.

1 is a schematic cross-sectional view of a multi-way valve according to a first embodiment of the present invention.
FIG. 2 is a schematic cross-sectional view taken along the line II-II of FIG. 1 to illustrate a state in which a tube is placed on a support plate.
FIG. 3 is a schematic cross-sectional view taken along line III-III of FIG. 1 to illustrate an arrangement state of the first supporting ground and the second supporting ground of the rotating plate.
4 is a schematic cross-sectional view of a multi-way valve according to a second embodiment of the present invention.
FIG. 5 is a schematic cross-sectional view taken along line V-V of FIG. 4 to illustrate a state in which a tube is placed on a second plate.
FIG. 6 is a schematic cross-sectional view taken along line VI-VI of FIG. 4, illustrating a state in which a tube is placed on a first plate.
FIG. 7 is a schematic cross-sectional view taken along line VII-VII of FIG. 4, to illustrate an arrangement state of the first supporting ground and the second supporting ground of the rotating plate.
8 is a schematic diagram of a regenerative cell extraction system equipped with a multi-way valve.
FIG. 9 is a schematic cross-sectional view of the first unit shown in FIG. 8.
FIG. 10 is a schematic exploded perspective view of the second unit shown in FIG. 8.
FIG. 11 is a schematic cross-sectional view of FIG. 10.
12 is a view for explaining a process of collecting and discharging the separated material using the vowel member and the guide member in the second unit.
13 is a diagram for explaining a multiple filter.
14 is a schematic cross-sectional view for explaining a modified embodiment of the multi-way valve according to the present invention.

Hereinafter, with reference to the accompanying drawings, a multi-way valve and a regenerated cell extraction system using the same according to an embodiment of the present invention will be described in more detail.

First, a multiway valve according to a first embodiment of the present invention will be described.

1 is a schematic cross-sectional view of a multi-way valve according to a first embodiment of the present invention, FIG. 2 is a schematic cross-sectional view taken along line II-II of FIG. 1 to describe a state in which a tube is placed on a support plate, and FIG. It is a schematic sectional drawing of the III-III line | wire of 1, and is a figure for demonstrating the arrangement state of the 1st ground surface and the 2nd ground surface of a rotating plate.

1 to 3, the multi-way valve 500 according to the first embodiment includes a main body 510, a cover 520, a rotation plate 530, and a shutter member 550.

The main body 510 includes a base plate 511 and a support plate 512. The support plate 512 is mounted on the base plate 511 and coupled to each other by screws (not shown). The interior of the body 510 is formed with a space to be installed the rotating plate 530 to be described later. In addition, one side of the base plate 511 is formed with an insertion hole 511a into which the central portion 535 of the rotating plate 530 and the respective rotation shafts 535a, 536a, and 527a of the outer ring portions 536 and 537 can be inserted.

A plurality of tubes 600 are placed and supported on the upper surface of the support plate 512. The fluid is transferred through the inside of the tube 600, the tube 600 is made of a flexible material that can be bent and compressed. In this embodiment, the tube 600 is composed of two sets, one tube set consists of three tubes (601, 602, 603) to meet each other at one point, the other tube set is one tube 604 Pass the support plate 512 and the other tube 605 is connected to this tube 604.

However, the arrangement of the tubes shown in FIG. 2 is merely an example, and the arrangement of the tubes may be changed into various shapes as necessary.

And the upper surface of the support plate 512 is formed with a seating groove 513 in which a plurality of tubes 600 can be seated. That is, a groove is formed concavely in the upper surface of the support plate 512 and the tube 600 is accommodated in the seating groove 513.

Meanwhile, a plurality of through holes 514 are formed in the support plate 512. The through hole 514 is formed at a position corresponding to the position where the plurality of tubes 600 are disposed. In more detail, each through hole 514 is formed to penetrate between the bottom surface of the seating groove 513 in which the tubes 600 are accommodated and the bottom surface of the support plate 512. However, the seating groove is not formed on the support plate 512 and the seating recess may be formed on the cover to be described later, or there may be no seating recess itself. In this case, as shown in FIG. Can be formed.

In addition, as shown in the enlarged view of FIG. 1, the through hole 514 is formed to be stepped and divided into an upper hole 515 and a lower hole 516. The upper hole 515 is formed in a small diameter, the lower hole 516 is formed in a large diameter and the locking step 517 is formed at the boundary between the upper hole 515 and the lower hole 516.

The cover 520 is for opening or closing the upper surface of the body 510 to the outside. In the present embodiment, the cover 520 is hingedly coupled to one side of the main body 510 to cover the upper surface of the main body 510 when rotating in one direction and to expose the upper surface of the main body 510 to the outside when rotating in the other direction. Let's do it.

A convex protrusion (not shown) is formed on the lower surface of the cover 520. The protrusion (not shown) is formed to correspond to the seating groove 513 formed in the support plate 512, the protrusion (not shown) when the cover 520 is closed in the state in which the tube 600 is accommodated in the seating groove 513 ) Is in close contact with the top of the tube (600).

In addition, the cover 520 is provided with a hook (not shown) to prevent the opening of the body 510 by an unwanted external force in a closed state. The hook (not shown) is rotatably coupled to the cover 520 to be caught by a hook portion (not shown) formed in the main body 510. A coil spring (not shown) is mounted at a portion where the hook (not shown) is hinged to elastically press the hook toward the hook portion, thereby maintaining the coupling between the cover 520 and the main body 510. When the cover 520 is to be opened, the hook may be rotated using a handle (not shown) to detach the hook from the hook portion.

The cover 520 may be used as a disposable article, unlike the main body 510. That is, the disposable cover 520 may be detachably coupled to the main body 510. Important point is provided with a seating groove or a clamping member (not shown) to secure the tube on the lower surface of the cover 520, the tube 600 is mounted on the cover 520, and then discarded the tube with the cover It's easy to use. In addition, even if the cover is not used for single use, a clamping member (eg, a ring into which a tube is fitted) attached to the lower surface of the cover may be used for single use.

On the other hand, the shutter member 550 acts as a valve for opening and closing the conduit in the tube 600 by pressing or releasing the tube 600 and is inserted into the through hole 514 formed in the support plate 512. Is installed.

That is, the shutter member 550 is inserted into the through hole 514 and protrudes from the upper surface of the support plate 512 (protrudes from the bottom surface of the seating groove) to protrude the tube 600 of the flexible material (not shown). The tube 600 is closed by squeezing toward it. On the contrary, when the shutter member 550 is immersed into the through hole 514, the compressed tube 600 is restored to a circular shape again to open the inner conduit.

The shutter member 550 may be installed in all the through holes 514 of the support plate 512, but if not installed in one or more through holes as necessary, some of the tubes 600 of the plurality of tubes 600 are always connected to the pipe. It can be kept open.

In the present embodiment, the shutter member 550 is elongated in the vertical direction. In particular, the upper portion is formed with a rod-shaped pressing portion 551 having a narrow diameter, and the lower portion of the pressing portion 551 has a wide diameter body portion ( 552 is formed. The pressing unit 551 may be fitted into the upper hole 515 of the through hole 514, and the body 552 may be fitted into the lower hole 516 of the through hole 514.

Since the diameters of the pressing portion 551 and the body portion 552 are different, a stepped portion 553 is formed between the pressing portion 551 and the body portion 552. Since the stepped portion 553 is caught by the locking jaw 517 of the through hole 514, the displacement of the shutter member 550 in the upward direction is limited. In addition, the end portion of the pressing portion of the shutter member 550 is linear. It can be formed to effectively press the tube 600.

A bearing 557 or a roller is rotatably installed under the shutter member 550. The bearing 557 is for reducing friction between the rotating plate 530 and the rotating plate 530 through the rolling contact to be described later.

A spring 519 is inserted between the stepped portion 553 of the shutter member 550 and the locking jaw 517 of the through hole 514 to support the spring 519 in the downward direction. Elastic bias.

Meanwhile, in the present invention, the rotating plate 530 is used as a driving means for supporting the shutter member 550 having the above-described configuration and moving up and down in the vertical direction.

The rotating plate 530 is composed of a central portion 535 and one or more outer ring portions. In this embodiment, two outer ring portions 536 and 537 are provided. The central portion 535 is rotatably installed on the base plate 511 via the bearing 535b. The outer ring portion 536 is formed in an annular shape and surrounds the central portion 535.

In the present embodiment, the outer ring portions 536 and 537 are formed with a stepped upper plate portion p and a lower plate portion k, and a connection portion is formed therebetween. The upper plate portion p forms the upper surface of the outer ring portions 536 and 537, and the lower plate portion k forms the lower surface of the outer ring portions 536 and 537. A flange portion 535f is also formed at the lower portion of the central portion 535, and a bearing 535c is interposed between the flange portion 535f of the central portion 535 and the upper plate portion p of the inner outer ring portion 536. In addition, a bearing 536b is interposed between the lower plate portion k of the inner outer ring portion 536 and the upper plate portion p of the outer outer ring portion 537, and the flange portion 511f and the outer outer ring portion of the base plate 511. The bearing 537b is also interposed between the lower plate portions k of 537.

As such, the central portion 535 and the outer ring portions 536 and 537 are supported between the base plate 511 and the support plate 512 through bearings, respectively, and can be stably rotated without change in posture and position.

Motors (not shown) and gears are used to rotate the central portion 535 and the outer ring portions 536 and 537. That is, gear teeth are formed on the inner circumferential surface or outer circumferential surface of the central portion 535 and the outer ring portions 536 and 537. In this embodiment, gear teeth are formed on the inner circumferential surfaces of the central portion 535 and the outer ring portions 536 and 537. Cylindrical drive gear 538 is geared to the gear. The cylindrical drive gear 538 is connected to a motor (not shown) through the rotation shaft 539. When the driving gear 538 is rotated according to the forward and reverse rotation of the motor, the center portion 535 and the outer ring portions 536 and 537 are rotated individually.

The upper surface of the rotating plate 530 is divided into a first support ground 532 and a second support ground 533. That is, the first supporting surface 532 and the second supporting surface 533 are formed on the upper surface of the rotating plate 530 along the circumferential direction with the rotation axis 531 of the rotating plate 530 as the center point. The first ground surface 532 is disposed at a first height, and the second ground surface 533 is disposed at a second height lower than the first height. Here, the first height is the height to support the shutter member 550 so that the shutter member 550 protrudes from the through hole 514 to press the tube 600, the second height is the shutter member 550 is Refers to a height for supporting the immersion into the through hole 514.

That is, when the rotation plate 530 is rotated, the shutter member 550 is alternately supported on the first support ground 532 and the second support ground 533 of the rotation plate 530. However, since the first support ground 532 and the second support ground 533 are different in the arrangement height thereof, the shutter member 550 is raised when supported by the first support ground 532 so that the through hole 514 is supported. It protrudes upward and descends when supported by the second support surface 533 to be immersed in the through hole 514.

As the shutter member 550 is raised and lowered, the tube 600 can be opened and closed. When the shutter member 550 is raised, the tube 600 is compressed to close the conduit, and when it is lowered, the tube 600 is elastically restored to open the conduit.

In the present embodiment, as shown in FIG. 3, the first supporting surface 532 and the second supporting ground 533 which are convexly protruding from the central portion 535 and the two outer ring portions 536 and 537 are respectively concave. Is formed. In the present exemplary embodiment, the first supporting surface 532 is formed at the center portion 535 at an approximately 250 ° angle along the circumferential direction, and the second supporting ground 533 is formed at the remaining portion. The first supporting surface 532 and the second supporting surface 533 are formed by dividing the upper surface of the inner outer ring portion 536. The first outer surface 532 is formed symmetrically with respect to the outer outer ring portion 537 at intervals of 180 degrees, and a second ground surface 533 is formed between the first ground surfaces 532.

The shutter members 550 are alternately supported and lifted on the first ground surface 532 and the second ground surface 533 as the center portion 535 or the outer ring portions 536 and 537 rotate. The connection portion between the second support surface 533 is configured to have a gentle inclination so that the lifting and lowering of the shutter member 550 can be performed smoothly.

In addition, the first support ground 532 and the second support ground 533 illustrated in FIG. 3 are merely examples, and the first support ground 532 and the second support ground 533 may include the tubes 600. In this case, the through hole 514 may be assigned in various ways depending on the situation and conditions under which the through hole 514 is disposed.

In the rotating plate 530 according to the above configuration, the amount of rotation of the motors installed in the central portion 535 and the outer ring portions 536 and 537 is adjusted, so that the specific shutter member 550 is made of the paper surface 532 or the second paper surface ( By being supported by 533, the pipeline of the tube 600 corresponding to the shutter member 550 can be opened and closed.

Hereinafter, with reference to the drawings, a multi-way valve 400 according to a second embodiment of the present invention will be described in detail.

FIG. 4 is a schematic cross-sectional view of a multi-way valve according to a second embodiment of the present invention. FIG. 5 is a schematic cross-sectional view taken along the line V-V of FIG. 4 to explain a state in which a tube is placed on a second plate. 4 is a schematic sectional view taken along the line VI-VI of FIG. 4, which is a view for explaining a state in which the tube is placed on the first plate, and FIG. 7 is a schematic sectional view taken along the line VII-Ⅶ of FIG. It is a figure for demonstrating the arrangement state of 2 ground surfaces.

Comparing the second embodiment and the first embodiment, the second embodiment differs from the first embodiment in that the support plate consists of two parts of the first plate and the second plate, and only one outer ring part is disposed. Only the difference is that most of the configuration is the same. The second embodiment will be mainly described with respect to the parts different from the first embodiment, and the same configuration as that of the first embodiment will be replaced with the description of the first embodiment.

4 to 7, the configuration of the multi-way valve 400 according to the second embodiment of the base plate 411, the cover 420 is the same as the first embodiment.

The support plate of the second embodiment consists of a first plate 412 and a second plate 413. The first plate 412 is fixed to the base plate 411 while maintaining a gap in the vertical direction with the rotation plate 430, the second plate 413 is detachably coupled on the first plate 412. . A seating groove h is also formed on the first plate 412 and the second plate 413 to install a plurality of tubes.

The first plate 412 is supported by a plurality of first tubes are fitted in the mounting groove (h), in this embodiment ten first tubes (651 ~ 660) are provided. The ten first tubes 651 to 660 are all connected to each other at a central point. Through holes 414 are formed in the lower portions of the first tubes 651 to 660. This through hole 414 also has the same configuration as in the first embodiment, and the shutter member 450 and the spring 419 are also fitted.

The second plate 413 is provided with a plurality of second tubes 631 to 633 and a plurality of independent tubes 641 to 643. In the present embodiment, three second tubes 631 to 633 are provided, and the second tubes 633 communicate with each other at the central point of the second plate 413. In the present embodiment, three independent tubes 641 to 643 are provided separately from the second tubes, and independent tubes 641 and 643 disposed at both sides are connected to the independent tubes 642 disposed at the center, respectively. .

The through holes 418 are formed at the lower sides of the second tubes and the independent tubes, respectively, and the through holes 418 communicate with the through holes 414 formed in the first plate 412. That is, referring to FIG. 6, the first plate 412 has a through hole 414 disposed below the first tubes 651 ˜ 660 and a through hole disposed outside the first tube. 414 (indicated by a solid line), the through hole 418 formed in the second plate 413 is disposed in mutual communication with the through hole 414 disposed outside the first tube in the first plate 412. .

In the first plate 412, the shutter member 450 is also inserted into the through hole 414 disposed outside the first tube. The pressing member 415 of the shutter member 450 is formed to be long, and thus the shutter member ( The pressing part 415 of the 450 is fitted from the through hole 414 of the first plate 412 to the through hole 418 of the second plate 413. When the shutter member 450 is raised, it protrudes with respect to the upper surface of the second plate 413 to compress the second tubes 631 to 633 or the independent tubes 641 to 643 to close the conduit.

The rotating plate 430 of the second embodiment is provided with only a central portion 435 and one outer ring portion 436. The central portion 435 and the outer ring portion 436 are rotatably supported by the respective bearings 435b, 435c, and 436b, and gears are formed on the inner circumferential surfaces of the central portion 435 and the outer ring portion 436 to drive the gears 438. ) Is also the same as the first embodiment. Of course, the drive gear 438 is connected to a motor (not shown) through the rotation shaft 439.

Referring to FIG. 7, in the rotating plate 430 of the second embodiment, a first supporting surface 432 and a second supporting ground 433 are formed in the central portion 435 and the outer ring portion 436 along the circumferential direction, respectively. .

The first support ground 432 and the second support ground 433 formed on the outer ring portion 436 support the shutter members for opening and closing the first tubes 651 to 660 disposed on the first plate 412. That is, since the position of the through hole 414 formed under the first tubes 651 to 660 is an area in which the outer ring portion 436 is positioned in the rotating plate 430, the shutter members fitted to the through hole 414 are formed. It is supported by the upper surface of the outer ring part 436.

Referring to FIG. 7, the outer ring portion 436 has most of its area allocated to the first supporting surface 433 along the circumferential direction, and only a very small area is allocated to the second supporting ground 433. The second support surface 433 of the outer ring portion 436 of the plurality of through holes formed in the region corresponding to the outer ring portion 436, that is, the through holes 414 disposed under the first tubes 651 to 660. In the upper part of), one through hole 414 is always arranged regardless of the rotational position of the outer ring part 436. That is, no matter where the outer ring portion 436 is rotated, the second support surface 433 supports only one shutter member 450 to open only one first tube of the plurality of first tubes 651 to 660. The remaining shutter members 450 are supported by the first support surface 432 of the outer ring portion 436, and thus all other first tubes are closed.

In addition, the first support ground 432 and the second support ground 433 formed at the central portion 435 of the rotating plate 430 may include the second tubes 631 to 633 and the independent tubes placed on the second plate 413. Supporting shutter members for opening and closing (641 ~ 643). Since the positions of the through holes 418 formed at the lower portions of the second tubes 631 to 633 and the independent tubes 641 to 643 are regions where the central portion 435 is located in the rotation plate 430, the through holes 418 are provided. The shutter members inserted in the) are supported on the upper surface of the central portion 435.

In the present exemplary embodiment, the first support ground 432 and the second support ground 433 of the central portion 435 are allocated in half at the central portion 435. That is, the upper surface of the central portion 435 is a circular ring shape, the first ground surface 432 (solid line) is half of the second ground surface 433 (dotted line) occupy the other half.

Therefore, of the plurality of through holes 418 formed in the region corresponding to the center portion 435 of the rotating plate 430, the upper portion of the first support ground 432 and the second support ground 433 of the central portion 435. In the upper portion, half through holes 418 are disposed, respectively, regardless of the rotational position of the central portion 435. As such, the first ground surface and the second ground surface are allotted in half and the plurality of tubes of the second tubes 641 to 643 and the independent tubes 641 to 643 placed on the second plate 413 are allocated. Can be opened and closed together.

So far, the multi-way valves 400 and 500 according to the first and second embodiments have been described. In the above embodiments, bearings are provided between the center portion and the outer ring portion or between the outer ring portion and the outer ring portion of the rotating plates 430 and 530. Although described and illustrated as being interposed, the bearing need not necessarily be interposed as described above, and may be constructed in a simpler manner. A variant of this form is shown in FIG. 14.

14 is a schematic cross-sectional view for explaining a modified embodiment of the multi-way valve according to the present invention.

Referring to FIG. 14, the outer ring portion 436 surrounds the center portion 435 in the same form, but the center portion 435 and the outer ring portion 436 are not connected to each other by bearings as in the previous embodiments. Arranged independently, annular plate bearings b1 and b2 are provided below the central portion 435 and the outer ring portion 436, respectively. That is, the upper plate of the plate bearing (b1, b2) is coupled to the central portion 435 or the outer ring portion 436, the lower plate is coupled to the base plate 411 so that the rotation plate 430 can be stably rotated . When the bearings are connected together with the outer ring portion and the center portion as shown in the above-described embodiment, the rotational speed may be too high when the center portion and the outer ring portion are relatively rotated, and thus the center portion and the outer ring portion may be rotated independently of each other. It was made.

In addition, although the second embodiment is illustrated in FIG. 14, the above configuration may be applied to the first embodiment as it is. In the case where there are a plurality of outer ring parts as in the first embodiment, bearings are interposed between the outer ring part and the outer ring part. Can be.

Hereinafter, an embodiment of a regenerative cell extraction system using a multi-way valve having the above configuration will be described.

8 is a schematic diagram of a regenerative cell extraction system equipped with a multi-way valve.

The regenerated cell extraction system 300 according to an embodiment of the present invention includes a first unit 100, a second unit 200, a multiway valve 400, and a parastatic pump 900.

The regenerated cell extraction system 300 is for separating tissues or separating tissues to extract regenerated cells such as stem cells. Tissues to be separated vary from adipose tissue to umbilical cord blood, etc. Hereinafter, fat tissue will be described as an example.

FIG. 9 is a schematic cross-sectional view of the first unit shown in FIG. 8. Referring to FIG. 8, the first unit 100 is for stirring and specific gravity separation of cell tissues such as fat tissue, and includes a sub container 110 and an entrance tube 130.

The sub container 110 includes a sub container ball 111 and a sub container cap 112 coupled to an upper portion of the sub container ball 111, and a space portion 113 in which a fatty tissue can be accommodated. ) Is formed.

  The lower part of the sub container ball 111 is formed in a shape of decreasing diameter toward the lower side, the groove portion 114 is formed concave at the lower end of the sub container ball 111.

The through hole 115 is formed in the center portion of the sub container cap 112, and the protruding wall portion 116 is formed to protrude upward from the outer circumference of the through hole 115.

 In addition, the stirring blade 117 is formed on the inner wall of the lower portion of the sub container ball 111. The stirring blades 117 are formed to protrude toward the space portion 113 from the inner wall, and the four are arranged symmetrically at intervals of 90 degrees with respect to the center of rotation of the sub-container 110. Of course, there may be only one stirring blade 117, it is not necessarily symmetrical. The stirring blade 117 is rotated together in accordance with the rotation of the sub-container 110, serves to smoothly stir adipose tissue.

The entrance tube 130 functions as a flow path for transferring the washing liquid, the enzyme, or the like to the space 113 of the sub container 110, or for discharging the material in the sub container 110 to the outside. The entrance tube 130 is formed in a hollow shape and is inserted into the sub container 110 through the through hole 115 of the sub container 110. The lower end of the entrance tube 130 is disposed in the groove 114 of the sub container 110 in a state of being close to the bottom of the sub container 110, and the upper end of the entrance tube 130 is positioned above the sub container 110. Is placed.

In addition, in the present embodiment, an auxiliary pipe 140 is installed to communicate the inside and the outside of the sub container 110 in an independent path from the access pipe 130. The auxiliary pipe 140 functions as a passage through which air flows into and out of the sub container 110. Therefore, the auxiliary pipe 140 does not need to be inserted to the lower end of the sub container 110, and only needs to be inserted to the upper end of the sub container 110.

The entrance tube 130 and the auxiliary tube 140 may be formed separately, but are integrally formed in this embodiment. That is, the entrance tube 130 is disposed long in the vertical direction, the upper portion is formed to be bent, the auxiliary tube 140 is formed with a larger diameter than the entrance tube 130 is wrapped around the entrance tube 130. The upper part of the auxiliary pipe 140 is formed by bending in the direction opposite to the direction in which the entry pipe 130 is folded. An interior of the auxiliary pipe 140, more precisely, a space between the inner circumferential surface of the auxiliary pipe 140 and the outer circumferential surface of the entrance pipe 130 forms a flow path 145 through which air can flow. However, the flow path 145 is not necessarily used only for the inflow and outflow of air, and may be used as the inflow and outflow path of various materials, such as to discharge substances such as blood contaminants or inject a washing solution, if necessary.

In addition, an insertion hole 135 is formed at an upper end of the entrance tube 130, and a stopper 136 is screwed to the insertion hole 135. When the stopper 136 is removed, a syringe needle (not shown) may be inserted into the entrance tube 130 through the insertion hole 135 to suck the liquid substance placed in the groove 114 of the sub container 110. . When the syringe needle is not used, the insertion hole 135 is closed by the stopper 136 to prevent contamination of the space 113 inside the sub container 110.

  And, the lower side of the auxiliary pipe 140, the shielding member 147 is fitted to the entrance pipe 130 is coupled. When the sub container 110 is rotated to separate the adipose tissue, a liquid substance such as blood or a washing solution may be rapidly raised to the upper part of the sub container 110, and these materials may be internally or auxiliary to the auxiliary pipe 140. It may be introduced into the through hole 115 between the tube 140 and the sub container 110. The shielding member 147 surrounds the through hole 115 and the auxiliary tube 140 to prevent blood contaminants from being introduced into the auxiliary tube 140. However, since the auxiliary pipe 140 must be through the space portion 113, the wrapping here is not meant to be completely sealed, and a gap d is formed at the upper portion so as to communicate with the space portion 113.

In addition, spiral outer auxiliary stirring blades (not shown) are formed on the outer circumferential surface of the entrance tube 130 to stir adipose tissue together with the lower stirring blades of the sub container 110.

On the other hand, the sub-container 110 should be rotated relative to the entrance tube 130 and the auxiliary pipe 140, the entrance tube 130 and the auxiliary pipe 140 and the sub container 110 is sealed to the sub container ( The space 113 of the 110 should be sealed.

In this embodiment, the compressive sealing member 150 and the friction member 160 are employed to allow the space 113 to be sealed while the sub container 110 is rotated. That is, the sealing member 150 employed in the present embodiment is formed in an annular shape and fitted to the protruding wall portion 116 of the sub container cap 112, and the flange portion 148 formed on the outer circumferential surface of the auxiliary pipe 140. It is interposed between the sub container caps 112. The sealing member 150 is made of an elastically compressible rubber material, and is compressed between the through hole 115 and the auxiliary pipe 140 when the sealing member 150 is compressed between the flange portion 148 of the auxiliary pipe 140 and the sub container cap 112. The gap between is completely sealable.

However, when the sealing member 150 is rotated together with the sub container 110, the rubber sealing member 150 has a high coefficient of friction and not only smooth rotation due to friction between the flange portion 148, but also high Friction heat is generated. The upper side of the sealing member 150 is attached to the friction member 160 of the material having a low coefficient of friction and high heat transfer coefficient. In this embodiment, the friction member 160 made of a ceramic material and formed in an annular shape is attached to the upper portion of the sealing member 150, and the friction member 160 is in close contact with the flange portion 148 of the auxiliary pipe 140. Surface contact.

However, in another embodiment, a retaining ring (not shown) and a bearing (not shown) may be used to achieve relative rotation and sealing between the second auxiliary pipe 140 and the sub container 110.

In addition, in the present embodiment, since the auxiliary pipe 140 has a structure in which the entrance pipe 130 and the auxiliary pipe 140 are integrally formed in a form surrounding the entrance pipe 130, the outer circumferential surface of the auxiliary pipe 140 and the through hole ( Although the sealing between the 115 is a problem, in other embodiments, the auxiliary pipe 140 may be formed separately from the entrance pipe 130 or may not be employed. Even in this embodiment, the entrance tube 130 is fitted to the through hole 115, in order to ensure the sealing and relative rotation between the entrance tube 130 and the through hole 115, on the outer circumferential surface of the entrance tube 130 A flange portion (not shown) is formed, and the sealing member 150 and the friction member 160 may be installed between the flange portion (not shown) and the sub container cap 112 formed on the outer circumferential surface of the access pipe 130.

The configuration of the first unit 100 has been described above. Hereinafter, the second unit 200 will be described in more detail with reference to the accompanying drawings.

FIG. 10 is a schematic exploded perspective view of the second unit shown in FIG. 8, and FIG. 11 is a schematic cross-sectional view of a state in which the second unit shown in FIG. 8 is coupled.

10 and 11, the second unit 200 is primarily separated from the first unit 100 to receive an aqueous solution containing stem cells, and extract stem cells from the aqueous solution.

The second unit 200 performing the above function includes a main container 210, a first discharge pipe 220, and a second discharge pipe 230.

The main container 210 is provided with a main body 211 and a cover 212, the receiving portion 213 is formed to receive the separation object such as an aqueous solution containing adipose tissue or stem cells therein. The lower portion of the main body portion 211 is gradually formed narrower from the upper side to the lower side or is formed in a constant diameter, in particular, the recessed portion is formed in the lowermost end of the receiving portion 213.

A through hole 215 penetrating between an upper surface and a lower surface is formed in the center of the upper surface of the cover 210, and an annular outer wall portion 216 is formed to protrude upward from an outer circumference of the through hole 215.

In addition, the protruding accommodation portion 217 is formed convexly on the outside of the main container 210. That is, the protrusion accommodating part 217 is convex toward the outside of the main container 210 in the radial direction about the center of rotation of the main container 210 and is formed long along the height direction of the main container 210.

Also, in order not to affect the rotation of the main container 210, the protrusion accommodation portion 217 should be disposed symmetrically to the main container 210, and in this embodiment the center of rotation (c) of the main container 210 Four positions are arranged symmetrically at 90 ° angle intervals.

When the main container 210 is rotated, the separation object is separated by weight, and the heavy component is disposed outside in the main container 210 and the light component is disposed inside. Since the protrusion accommodating part 217 is disposed at the outermost side with respect to the center of rotation of the main container 210, the separation of the heaviest component is accommodated in the protrusion accommodating part 217. Stem cells isolated from adipose tissue are heavier than other components and thus are accommodated in the protruding portion 217 during centrifugation. This will be described later in detail.

On the other hand, the lower end of the protrusion receiving portion 217 is blocked by the blocking member 240. That is, stem cells should be accommodated in the protrusion accommodation portion 217 by centrifugation. If the lower end portion of the protrusion accommodation portion 217 is in communication with the bottom portion of the main container, the separation object may be separated before the centrifugation is properly performed. It is raised from the bottom of the container 210 to the protrusion receiving portion, and the stem cell attached to the protrusion receiving portion 217 may be lost as the aqueous solution hits up. Thus, stem cells attached to the protrusion receiving portion 217 can be stably received, the blocking member 240 is installed to prevent the loss of the stem cells.

The first discharge pipe 220 and the second discharge pipe 230 are both for communicating the receiving portion 213 of the main container 210 and the outside of the main container 210, the first discharge pipe 220 and the second Discharge pipe 230 forms a mutually independent path. The first discharge pipe 220 and the second discharge pipe 230 is disposed coaxially with the main container 210, the first discharge pipe 220 is formed long so that the lower end of the lower end of the main container 210, more precisely Although disposed in the groove portion 214, the second discharge pipe 230 is short and the difference is that the lower end is disposed in the upper end of the main container (210).

The material flowing through the first discharge pipe 220 and the second discharge pipe 230 may be changed according to the use type, but in the present embodiment is separated from the first unit 100 through the first discharge pipe 220. An aqueous solution and a washing solution containing stem cells may be introduced, and the stem cells or the washing solution extracted from the main container 210 may be discharged. In addition, the air may flow in and out through the second discharge pipe, and the cleaning solution after use may be discharged.

Specific configuration of the first discharge pipe 220 and the second discharge pipe 230 is made of the same configuration as the entrance pipe 130 and the auxiliary pipe 140 in the first unit (100). That is, the first discharge pipe 220 is the same as the access pipe 130 of the first unit 100, the second discharge pipe 230 is the same as the auxiliary pipe 140.

The first discharge pipe 220 and the second discharge pipe 230 may be formed separately, but are integrally formed in this embodiment. That is, the first discharge pipe 220 is disposed long in the vertical direction and the upper portion is formed to be bent, the second discharge pipe 230 is formed with a larger diameter than the first discharge pipe 220 to surround the first discharge pipe 220 Is placed. An upper portion of the second discharge pipe 220 is formed by bending in a direction opposite to that of the first discharge pipe 220. The interior of the second discharge pipe 230, more precisely, the space between the inner peripheral surface of the second discharge pipe 230 and the outer peripheral surface of the first discharge pipe 220 forms a flow path 235 through which fluid such as air, cleaning liquid can flow. do.

In addition, an insertion hole 237 is formed at an upper end of the first discharge pipe 220, and a cap 236 is screwed to the insertion hole 237. When the cap 236 is removed, an auxiliary discharge pipe (not shown) is inserted into the first discharge pipe 220 through the insertion hole 237 to be inserted into the groove 214 of the main container 210. The auxiliary discharge pipe (not shown) may be connected to the syringe to suck in the liquid substance placed in the groove 214 of the main container 210. Here, the secondary discharge pipe is used a needle connected to the syringe. When the auxiliary discharge pipe is not used, the insertion hole 237 is closed by the cap 236 to prevent the container 213 inside the main container 210 from being contaminated.

On the other hand, the main container 210 should be rotated relative to the first discharge pipe 220 and the second discharge pipe 230, the sealing between the first discharge pipe 220 and the second discharge pipe 230 and the main container 210. Thus, the receiving portion 213 of the main container 210 should be sealed.

In this embodiment, the medium member 250 and the contact member 260 are employed to seal the receiving portion 213 while the main container 210 is rotated. That is, the intermediate member 250 employed in the present embodiment is formed in an annular shape and fitted to the outer wall portion 216 of the main container cover 212, and the flange portion 238 formed on the outer circumferential surface of the second discharge pipe 230. And the main container cover 212. The intermediate member 250 is made of an elastically compressible rubber material. When the intermediate member 250 is compressed between the flange portion 238 of the second discharge pipe 230 and the main container cover 212, the through hole 215 and the second discharge pipe 230 are formed. The gap between) is completely sealable.

However, when the intermediate member 250 is rotated together with the main container 210, the rubber intermediate member 250 has a high coefficient of friction, and thus the smooth rotation is not performed due to friction between the flange 248. Friction heat is generated. Accordingly, the contact member 260 of a material having a small friction coefficient and a high heat transfer coefficient is attached to the upper side of the intermediate member 250. In this embodiment, the contact member 260 made of a ceramic material and formed in an annular shape is attached to the upper portion of the intermediate member 250, and the contact member 260 is in close contact with the flange portion 238 of the second discharge pipe 230. Surface contact.

In addition, in the present embodiment, since the first discharge pipe 220 and the second discharge pipe 230 are formed in one body in a form in which the second discharge pipe 230 surrounds the first discharge pipe 220, the second discharge pipe 230 is formed. Although sealing between the outer circumferential surface and the through hole 215 is problematic, in another embodiment, the second discharge pipe 230 may be formed separately from the first discharge pipe 220 or may not be employed. Thus, even when the second discharge pipe 230 is not employed in the embodiment, since the first discharge pipe 220 is fitted into the through hole 215, the sealing between the first discharge pipe 220 and the through hole 215 and In order to ensure relative rotation, a flange portion (not shown) is formed on the outer circumferential surface of the first outlet pipe 220, and the intermediate member 250 and the contact member 260 are flange parts (not shown) on the outer circumferential surface of the first discharge pipe 220. And the sub container cover 212 may be provided.

On the other hand, in the present embodiment, while the main container 210 is rotated for centrifugation, a structure capable of discharging the washing solution other than stem cells to the outside of the regenerated cell extraction unit 100 through the second discharge pipe 230. to provide. That is, in the present embodiment, the rotation of the main container 210 may be stopped and the cleaning liquid may be discharged from the lower portion of the main container 210 through the first discharge pipe 220, but the main container 210 may be rotated. The cleaning liquid may be discharged to the outside by the vowel member 270 and the guide member 280 which will be described later.

The vowel member 270 is fitted to the first discharge pipe 220, and is provided with a concave collecting portion 271 so as to temporarily receive the separation guided by the guide member 280 to be described later. In the present embodiment, the vowel member 270 is formed in a concave disc shape. The vowel member 270 is disposed above the main container 210, and more precisely, is disposed closely to the lower end of the second discharge pipe 230. In addition, the diameter of the vowel member 270 is set to a size such that the outer circumferential surface of the vowel member 270 may be close to the inner circumferential surface of the main container 210.

The guide member 280 is disposed on the vowel member 270 to guide the separator that is being rotated in the centrifuged state inside the main container 210 to the collection portion of the vowel member 270. In the present exemplary embodiment, the guide members 280 are formed to protrude upward from the upper surface of the vowel member 270, and four guide members 280 are symmetrically disposed at intervals of 90 ° on the vowel member 270. In addition, the plurality of guide member 280 is formed toward the center from the outer peripheral surface of the vowel member 270 is arranged to be curved in a curve. Accordingly, the plurality of guide members 280 disposed on the vowel member 270 are formed in a toroidal shape as a whole.

Of course, the guide member 280 is not necessarily arranged to be curved, it may be a straight line, it is not formed over the entire radius of the vowel member 270 to be formed as a short section in contact with the separation material It may be in various forms, such as. In addition, the number of guide members may be variously selected from one to a plurality.

The separation object accommodated in the main container 210 is rotated while being pushed outward by the centrifugal force as the main container 210 is rotated. As described above, the stem cells, etc., which are heavy components, are formed in the protruding portion 217. It is accommodated, the light component such as the cleaning liquid maintains a state in close contact with the inner peripheral surface of the main container (210). When the main container starts to rotate, the separation object is rotated together to raise the water level from the bottom to the top of the main container 210. As shown in FIG. While being in contact with and interfering with the guide member 280, the guide member 280 is guided toward the center of the vowel member 270. When a suction force is applied to the second discharge pipe 230 from the pump, the separation guide guided to the center portion of the collection member 270 is discharged to the outside through the second discharge pipe 230 by this suction force.

In addition, a plurality of distribution holes 272 are formed in the central portion of the vowel member 270, and the distribution holes 272 lower the pressure applied to the vowel member 270 and a large amount of the separator may be introduced rapidly. Prevents the separator from overflowing.

Meanwhile, in the regenerative cell extraction system 300 according to the present invention, the multiway valve 400 described with reference to FIGS. 4 to 7 is employed. Therefore, the configuration of the multi-way valve 400 employed in the regenerative cell extraction system 300 will be replaced with the above description. However, the difference between the multi-way valve and the multi-way valve employed in the regenerative cell extraction system according to the second embodiment described above is different from the second tube indicated by reference numeral 632 and the lower side of the first tube indicated by reference numeral 651. The through hole is not provided with a shutter member. Therefore, regardless of the rotation of the rotating plate, the second tube indicated by reference numeral 632 and the first tube indicated by reference numeral 651 always remain open. Since the second tube denoted by reference numeral 632 and the first tube denoted by reference numeral 651 are interconnected, the plurality of first tubes 651 to 660 and the plurality of second tubes 631 to 633 are in communication with each other. .

Referring to FIG. 8, the arrangement of the plurality of tubes connected to the multiway valve 400 in the regenerative cell extraction system 300 will be described. In FIG. 8, the first plate 412 and the second plate 413 are illustrated as being independent from each other. However, this is for facilitating the description of the connection state of the respective tubes. Note that it is bound to (400).

Ten first tubes 651 to 660 placed on the first plate 412 of the multi-way valve 400 are installed in communication with each other as described above, and these first tubes are sequentially referenced from 651 to 660. Into the fermentation pump 900, the first discharge pipe 220 of the second unit 200, stem cell reservoir (b1), mature fat cells and oil reservoir (b2), wash solution reservoir (b3), enzyme storage Bag (b4), frozen stem cell storage bag (b5), adipose tissue storage bag (b6) to be treated, and washed with adipose tissue storage bag (b7), waste fluid storage bag (b8).

The three second tubes 631 to 633 disposed on the second plate 413 of the multi-way valve 400 are installed to communicate with each other, and the second tube denoted by reference numeral 631 is an entrance tube of the first unit 100. And 130. The second tube, indicated by reference numeral 632, is connected to the first tube, indicated by reference numeral 651, by connecting to the pump 900. The second tube, indicated by reference numeral 633, is connected to the first tube (connected with the first discharge tube of the second unit), indicated by reference numeral 652.

The three independent tubes 641 to 643 placed on the second plate 413 are installed in communication with each other, and the independent tubes indicated by reference numeral 641 are connected to the waste storage bag b8, and the independent tubes indicated by reference numeral 642. Is connected to the second discharge pipe 230 of the second unit 200, the independent tube indicated by the reference number 643 is connected to the filter (p2). The filter p2 prevents external polluted air from entering the second unit 200. The auxiliary pipe 140 of the first unit 100 is also connected to the filter p1 through the tube to prevent the contaminated air from flowing into the first unit from the outside.

As described above, in the regenerative cell extraction system 300 according to the present invention, the first unit 100, the second unit 200, and various storage bags b1 to b8 using one multi-way valve 400. Can be linked in various ways. In particular, in the present exemplary embodiment, the multi-way valve 400 is connected to the first plate 412 and the second in a situation in which the tube is not easily disposed by a plurality of tubes (first tube, second tube, and independent tube). The plate 413 is composed of two layers, and the tube is twisted or entangled. In addition, the first tubes 651 to 660 placed on the first plate 412 are opened and closed by the rotation of the outer ring portion 537, and the second tubes 631 to 633 and the independent tubes placed on the second plate 413. 641 to 643 are opened and closed by the central portion 536 to facilitate opening and closing of the tubes.

In addition, in the first tube 652 connected to the first discharge pipe 220 of the second unit 200, as shown in FIG. 13, a multi-filter (f) capable of filtering a large tissue such as collagen in a lump state Is installed. This multiple filter (f) is a stack of two mesh pockets, with a small pocket inside a large pocket. Openings are formed at one side of the multiple filter (f), so that liquid or small particles can pass through the multiple filter (f), but large tissue such as collagen can no longer flow through the multiple filter (f).

On the other hand, the photosensor (s) is installed in the second tube indicated by the reference number 632. The photosensor (s) can detect the color of the fluid flowing through the second tube 632, in particular the yellow fat tissue or fat cells discharged through the entrance tube 130 from the first unit (100) By detecting the color of the fat cells or adipose tissue is sent a signal to the controller (not shown) to be described later.

The pump 900 provides a suction force so that the fluid can be transferred through the first tubes 651 to 660, the second tubes 631 to 633, and the independent tubes 641 to 643. In other words, it provides power in transporting all substances, such as transporting substances such as enzymes and washing liquids, discharging fat cells and waste liquids to the outside, or moving extracted stem cells to storage bags. The pump used in this embodiment is a perilstatic pump that provides suction while rotating in one direction or in the reverse direction.

On the other hand, in the regenerated cell extraction system 300, the first unit 100 and the second unit 200 is mounted on a rotating jig (not shown) is rotated. A controller (not shown) for controlling the operation of components such as a rotating jig (not shown), a pump 900, a rotation plate 430 of the multi-way valve 400, and various sensors is provided.

In the regenerated cell extraction system 300 having the above configuration, the plurality of first tubes 651 to 660 by independently rotating the central portion 435 and the outer ring portion 436 of the rotating plate 430 of the multi-way valve 400. ), The plurality of second tubes 631 to 633 and the independent tubes 641 to 643 can be opened and closed. Thus, the fluid can be freely transferred by operating the pump 900 in a state of selectively opening and closing the plurality of tubes.

For example, if the cleaning liquid is to be transferred from the washing liquid storage bag b3 to the first unit 100, the second tube indicated by reference numeral 631 is rotated by rotating the center portion 435 on the rotating plate 430 of the multi-way valve 400. When the pump 900 is opened while the outer ring portion 436 is rotated and the first tube indicated by the reference numeral 655 is opened, the cleaning liquid passes through the first tube indicated by the marks 655 and 651, and then 632 and 631. It is introduced into the entrance tube 130 of the first unit 100 via the second tube indicated by. As described above, the second tube and the first tube, denoted by reference numerals 632 and 651, are not provided with a shutter member at the lower side thereof, and thus remain open at all times regardless of the rotation angle of the rotating plate.

For example, the process of transferring the aqueous solution containing stem cells from the first unit 100 to the second unit 200 is taken as an example. First, the central portion 435 of the rotating plate 430 is rotated by the controller so that the semicircular second support surface 433 is disposed under the tubes indicated by reference numerals 631, 641, 642 placed on the first plate 412. The upper tubes are opened by supporting the shutter member 450. In addition, the outer ring portion 436 is rotated to open the first tube indicated by reference numeral 652 placed on the second plate.

When the pump 900 is rotated in one direction in the above state, the aqueous solution of the lower part of the first unit 100 is discharged through the entrance tube 130, and then passes through the second tubes indicated by reference numerals 631 and 632, and then It is transferred to the first tube indicated by the numeral 651, and again flows into the first discharge pipe 220 of the second unit 200 through the tube indicated by the reference numeral 652. In the first unit 100, the air is introduced through the filter p1 when the aqueous solution is discharged, and in the second unit 200, the air discharged through the independent tube indicated by the reference numeral 642 is the reference number. It is transferred to the waste liquid storage bag b8 through an independent tube labeled 641.

The transfer process of the fluid is an example, and by rotating the central portion 435 and the outer ring portion 436 of the multi-way valve 400 to elevate the shutter member 450 and the various storage bags b1 to b8 Fluid can be transferred between the first unit 100 and the second unit 200.

Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims. Of course.

Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined not only by the claims below, but also by the equivalents of the claims.

100 ... first separation unit 200 ... second separation unit
300 ... regenerative cell extraction system 400,500 ... multi-way valve
410,510 ... Main unit 411,511 ... Base plate
412 ... First Plate 413 ... Second Plate
420,520 ... cover 430,530 ... rotating plate
432,532 ... First Ground 433, 533 ... Second Ground
435,535 ... center part 436,536,537 ... outer ring part
450,550 ... Shutter member 419,519 ... Spring
651 ~ 660 ... first tube 631 ~ 633 ... second tube
641 ~ 643 ... Independent tube 900 ... Pump

Claims (22)

A support plate having a plurality of tubes through which fluid is transferred is placed on an upper surface, and a through hole is formed in a lower portion of an area in which the plurality of tubes are disposed;
A cover that opens and closes an upper surface of the support plate;
A plurality of shutter members inserted into and liftable from the through-holes of the support plate to press the tube placed on the support plate to close or release the pressure to open the support plate; And
A rotating plate disposed below the support plate to support the shutter member, and having a central portion rotatably installed, and at least one outer ring portion rotatably disposed to surround the central portion sequentially and rotatably installed. With;
An upper surface of the center portion and the outer ring portion is formed of a first ground surface disposed at a first height and a second ground surface disposed at a second height lower than the first ground surface, so that the shutter member is supported by the first ground surface. The multi-way valve, characterized in that protruding from the through-hole to close the tube. The method of claim 1,
The support plate may include a first plate having a plurality of through holes formed therein, and a second plate having a plurality of through holes stacked on the first plate and connected to a part of the first plate.
The plurality of tubes are respectively placed on the first plate and the second plate, the cover opens and closes the upper surface of the second plate,
The shutter member fitted together in the through hole of the first plate and the through hole of the second plate which are connected to each other is formed longer than the shutter member inserted only in the through hole of the first plate, thereby supporting the first plate of the rotating plate. When supported on the surface of the multi-way valve, characterized in that protrudes with respect to the second plate. The method of claim 2,
The shutter member for opening and closing the tubes placed on the first plate is supported by either the central portion or the outer ring portion,
The shutter member for opening and closing the tubes placed on the second plate is supported by the other of the central portion or the outer ring portion. The method of claim 1,
Gears are formed on the outer peripheral surface or the inner peripheral surface of the central portion,
And a drive gear for rotating the center portion by being gear-coupled to and rotated by a gear formed at the center portion, and a motor for rotating the drive gear. The method of claim 1,
Gears are formed on the inner circumferential surface or outer circumferential surface of the outer ring portion,
And a drive gear for rotating the outer ring part by being gear-coupled to and rotated by a gear of the outer ring part, and a motor for rotating the drive gear. The method of claim 1,
And a bearing is interposed between the outer ring portions and between the outer ring portion and the central portion. The method according to claim 6,
The outer ring portion includes an upper plate portion forming an upper surface, a lower plate portion forming a lower surface, and a connection portion connecting the upper plate portion and the lower plate portion,
The bearing is a multi-way valve, characterized in that interposed between the upper plate portion of the outer ring portion of one side and the lower plate portion of the outer ring portion of the other side. The method of claim 1,
The through hole formed in the support plate is composed of an upper hole formed in a narrow area and a lower hole formed wider than the upper hole, and a locking step is formed between the upper hole and the lower hole.
The shutter member is a multi-way valve, characterized in that it has a pressing portion to be fitted to the upper hole and the body portion is fitted to the lower hole, the movement is limited to the upper locking hook. 9. The method of claim 8,
And a spring which is supported between the engaging jaw in the through hole and the body portion to elastically bias the shutter member downward. The method of claim 1,
It is coupled to the lower portion of the shutter member is a multi-way valve characterized in that it further comprises a bearing in contact with the upper surface of the rotating plate when the rotating plate is rotated. The method of claim 1,
At least one of the plurality of through holes formed in the support plate is left without the shutter member installed,
Multi-way valve, characterized in that the tube passing through the upper portion of the through-hole without the shutter member is always kept open. The method of claim 1,
Any one of the upper surface of the support plate or the lower surface of the cover is a multi-way valve, characterized in that the recessed groove portion is formed in which the plurality of tubes are seated. The method of claim 12,
The other one of the upper surface of the support plate or the lower surface of the cover is not formed in the seating groove portion is a multi-way valve, characterized in that the protrusion is formed to project toward the plurality of tubes seated in the seating groove. The method of claim 1,
The cover is detachably coupled to the support plate,
The lower surface of the cover is provided with a clamping member for fixing the plurality of tubes,
Multi-way valve, characterized in that the cover and the clamping member is used for single use or the clamping member is used for single use. The method of claim 1,
Bearings are interposed between the center portion and the outer ring portion, respectively, so that the center portion and the outer ring portion are rotated independently without being connected to each other. It is for separating the tissue (tissue), the sub-container is rotated by receiving a rotational force from the outside and the hollow portion is inserted into the sub-container so that one end is disposed below the sub-container, the space portion is formed; A first unit having an entrance tube;
A main container for extracting regenerated cells from the separation object transferred from the first unit, the main container is rotated by receiving a rotational force from the outside and the receiving portion is formed to accommodate the separation object, and one end is disposed below the main container A second unit having a hollow first discharge pipe inserted into the main container so that the other end thereof is disposed outside the main container;
Claims 1 to 15, wherein the configuration of any one of the first, the first pipe of the first unit, the first discharge pipe of the second unit and a plurality of storage bags are connected to each other; And
And a pump coupled to the multi-way valve. 17. The method of claim 16,
A plurality of first tubes are connected to each other, and a plurality of second tubes are connected to the support plate separately from the first tubes,
Regeneration cell extraction system, characterized in that the tube of any one of the first tube and any one of the second tube is interconnected through the pump. 18. The method of claim 17,
The first tubes are opened and closed by a shutter member supported by the outer ring portion in the support plate,
The second tube is regenerated cell extraction system, characterized in that the opening and closing by the shutter member is supported on the central portion in the support plate. 18. The method of claim 17,
Any one of the tubes of the first tube that is not connected to the pump is connected to the first discharge pipe of the second unit, the other tube is connected to the storage bags,
The first tube of any one of the second tube that is not connected to the pump is connected to the entrance pipe of the first unit, the other tube is connected to the first discharge pipe of the second unit Regenerated cell extraction system, characterized in that connected to the tube. 17. The method of claim 16,
The second unit further includes a hollow second discharge pipe inserted into the main container to communicate the main container with the outside in a separate path from the first discharge pipe,
Three independent tubes interconnected are installed on the support plate, wherein the three independent tubes are connected to the second discharge tube, the waste storage bag and the filter of the second unit, respectively. 21. The method of claim 20,
And the three independent tubes are opened and closed by a shutter member supported at the center portion of the support plate. 17. The method of claim 16,
Of the plurality of through holes formed in an area corresponding to the center portion of the rotating plate in the support plate, the upper portion of the first support surface and the second support surface of the central portion, respectively, irrespective of the rotation position of the rotation plate, respectively. The first ground plane and the second ground plane of the center portion of the rotating plate are allocated so that half through holes are arranged,
Rotation such that one through hole is disposed on an upper portion of the second supporting surface of the outer ring portion of the plurality of through holes formed in an area corresponding to the outer ring portion of the rotating plate on the support plate, regardless of the rotation position of the rotating plate. The regenerated cell extraction system, characterized in that the first ground surface and the second ground surface of the plate outer ring portion is assigned.

Images