KR101679671B1 - System for extracting regenerative cells - Google Patents

Abstract

A regenerative cell extraction system is disclosed. A regenerative cell extraction system according to an embodiment of the present invention includes a first unit, a second unit, and a transfer unit. The first unit collects tissue by vacuum inhalation, separates blood contaminants from the tissue harvested by stirring and centrifuging, and separates the cells from the separated tissue of the blood contaminant to suspend cells suspended in the liquid . The second unit is charged with cells suspended in the liquid from the first unit, separates the regenerative cells by centrifugation, and is smaller in capacity than the first unit. The transfer unit is connected between the first unit and the second unit and injects wash water into the first unit and the second unit and transfers the extract from the first unit and the second unit to the corresponding bags And the cells suspended in the liquid extracted from the first unit are injected into the second unit or the tissue and cell contaminants are transferred. The tissue is an adipose tissue, and the regenerating cell is an adipose-derived stem cell.

Description

[0001] SYSTEM FOR EXTRACTING REGENERATIVE CELLS [0002]

More particularly, the present invention relates to a regenerative cell extracting system, and more particularly, to a method and apparatus for extracting adipose tissue and separating regenerated cells from adipose tissue by an automated program in a closed state. In each step, pure adipose tissue, The present invention relates to a regenerative cell extracting system capable of selectively obtaining oil and fat-derived stem cells and easily regulating the amount of regenerated cells to be harvested.

Stem cells are defined as cells that have clonogenic and self-renewal capabilities that can differentiate into multiple cell lines under specific conditions. Embryonic stem cells are derived from mammalian embryos at the stage of blastocyst and have the ability to differentiate into almost all cells present in the body whereas adult stem cells are cells that are present in very small amounts in postnatal differentiated tissues, It is the cell that holds the ability of the cell. Adult stem cells offer real advantages over embryonic stem cells. Unlike embryonic stem cells, adult stem cells can be extracted from patients themselves without causing ethical problems. They are abundant in supply and are inherent in various tissues of the human body. The most available sources of adult stem cells are bone marrow, peripheral blood, cord / umbilical cord blood, and adipose tissue, as confirmed in recent studies. These cells can maintain, produce, and replace final differentiated cells in their own specific tissues as a result of physiological cell turnover or wound damage.

This ability, called cell plasticity, has led to the development of therapeutic applications aimed at the regeneration of defective tissues, with the aim of restoring the physiology and function of diseased organs. Adult stem cells can not only produce hematopoietic cells as known decades ago, but also can produce blood vessels, muscles, bones, cartilage, skin, nerves and the like, as recently discovered. These cells are known as mesenchymal stem cells. In addition, platelets made from platelet concentrates can be used to accelerate wound healing and, as a result, can play a role in regenerative medicine that helps in the reconstitution of tissues such as bones, skin or other tissues.

Recently, adipose tissue has been found to be a source of stem cells, progenitor cells and substrate materials suitable for therapeutic applications. Adipose tissue is also a rich source of vascular endothelial cells, which can play a role in tissue regeneration and tissue engineering by stimulating growth of new blood vessels and stimulating stem and progenitor cell growth.

However, although many devices have been developed for the collection of cells from adipose tissue, these devices do not adequately accommodate a suction device for collection of adipose tissue, or partial or complete automation from collection of adipose tissue to treatment of tissue . There is also the problem of the lack of a partial or complete sealing system from the collection of adipose tissue to the treatment of tissue and the resulting contamination problems.

Thus, the process from the collection of adipose tissue to the treatment of tissue is completely automated by the program in a closed state, and the purity of regenerating cells, that is, stem cells, taken from adipose tissue is improved, It is necessary to develop a method and apparatus for reducing the need for manipulation after extraction of cells.

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and it is an object of the present invention to provide a method and a device for producing a tissue- The present invention provides a regenerative cell extracting system that can be reduced to an amount that can be used immediately and reduces the necessity of separate operation after extraction of cells.

According to an aspect of the present invention, there is provided a regeneration cell extracting system including a first unit, a second unit, and a transfer unit.

The first unit collects tissue by vacuum inhalation, separates blood contaminants from the tissue collected by stirring and centrifuging, separates the cells from the separated tissue of blood contaminants, .

The second unit is charged with cells suspended in the liquid from the first unit, separates the regenerative cells by centrifugation, and is smaller in capacity than the first unit.

The transfer unit is connected between the first unit and the second unit and injects wash water into the first unit and the second unit and transfers the extract from the first unit and the second unit to the corresponding bags And the cells in a state suspended in the liquid extracted from the first unit are introduced into the second unit or the tissue and cell contaminants are transferred.

The tissue is an adipose tissue, and the regenerating cell is an adipose-derived stem cell.

The first unit includes a cylindrical upper body portion, a middle body portion integrally formed at a lower portion of the upper body portion and inclined toward the center toward the lower side, and a middle body portion integrally formed with the middle body portion, A first chamber having a lower body portion which is smaller than the inner diameter of the portion and extends with the same inner diameter and a lid portion which is integrally formed on the upper surface of the upper body portion and covers the upper surface, Wherein the first chamber and the second chamber are separated from each other by a predetermined distance from the bottom surface of the lower body part, the tissue being introduced into the first chamber through the upper part of the center tube, A central tube,

At least one first wing portion is formed in the direction of the center pipe to smooth agitation of the tissue collected on the inner wall surface of the middle body portion of the first chamber.

Wherein the first chamber surrounds the center pipe and is inserted into the guide hole and is formed integrally with the center pipe and has a negative pressure pipe for making the first chamber at a negative pressure state, A retainer is mounted between the sound pressure pipes so that the first chamber rotates independently of the center pipe and the sound pressure pipe while maintaining the hermeticity.

The lower end of the center pipe is spaced apart from the bottom of the lower body part by 0.1 mm to 3 mm on the bottom face. At least one auxiliary vane protruding outward is formed in a portion of the center tube inserted into the first chamber.

A bearing for smoothly rotating the first chamber may be further installed between the wall surface defining the guide hole and the sound pressure pipe together with the retainer, A filter capable of holding a foreign matter can be mounted inside the lower body portion of the lower portion of the main pipe.

Wherein the second unit comprises: an inlet portion having a predetermined diameter and extending vertically downward; and a cylindrical upper portion having an inner diameter increasing from the lower end toward the lower portion of the cell separating portion, A second chamber formed integrally with the lower portion of the upper body portion and extending downwardly and having a predetermined inner diameter, and a second chamber extending from the lower end of the inlet portion to the cell separating portion, A passage for discharging a physiological buffer solution other than the regenerating cells existing in the second chamber is formed between the upper plate and the upper plate, and the passage formed inside the inlet is formed to correspond to the inner surface of the upper plate, A first inner inlet portion having a first inner diameter and a second inner diameter larger than the first inner diameter, The second chamber having a first inner inlet portion and a second inner inlet portion having a first inner diameter and a second inner diameter; And a passage for discharging the regenerative cells formed on the bottom surface of the lower body portion of the second chamber, the passage being inserted into the second chamber through the inner side of the inlet tube and longer than the inlet tube Which is inserted between an outer wall surface of the second inner inlet portion and an inner wall surface of the inlet portion and discharged through a path between the upper plate portion and the lower plate portion, And a discharge pipe.

The second chamber is independently rotated by the retainer to maintain the hermeticity with the inlet tube, the first outlet tube, and the second outlet tube.

The inlet tube having a first support vane surrounding the outer wall surface of the first inner inlet section on the outside and a second support vane surrounding the inlet section,

The retainer is mounted between the first support vane and the inner wall surface of the second inner inlet portion and between the outer wall surface of the inlet portion and the second support vane to maintain airtightness while the second chamber rotates independently do.

A bearing is further provided between the first support vane and the inner wall surface of the second inner inlet portion and between the outer wall surface of the inlet portion and the second support vane to smooth the rotation of the second chamber together with the retainer do.

The injection pipe is inserted to the lower end of the upper body part, and the first discharge pipe is inserted to a height of 0.1 mm to 2 mm from the bottom face of the lower body part.

At least one second wing portion for smoothly stirring the second chamber when the second chamber rotates is formed in the inner wall surface from the cell separation portion to the lower end of the upper body portion in the direction of the injection tube.

The lower plate portion is integrally formed at the lower ends of the first inner inlet portion and the second inner inlet portion and the lower ends of the first inner inlet portion and the second inner inlet portion and has a slope equal to the slope of the upper plate portion, And at least one passage is provided between the upper plate and the lower plate, and a circular plate portion between the passage and the passage is bonded to the upper plate.

By adjusting the rotational speed of the second chamber and the length of the circular plate, it is possible to collect blood components according to the blood component layer formed inside by the centrifugal force of the second chamber, and the physiological buffer solution other than the regenerative cells Adjust the amount left in the chamber.

The lower plate portion is integrally formed at the lower ends of the first inner inlet portion and the second inner inlet portion and the lower ends of the first inner inlet portion and the second inner inlet portion and has a slope equal to the slope of the upper plate portion, A circular plate that extends and forms the passage with the top plate portion and a hollow flat plate shape surrounding the outside of the second inner inlet portion of the bottom plate portion and selectively connecting at least one passage among the passages to the second outlet pipe Wherein at least one passage is provided between the upper plate and the lower plate, a circular plate portion between the passage and the passage is bonded to the upper plate, and when the circular plate is divided in the radial direction, The weights of the respective half portions are equal to each other, the lengths of the passages formed in one half portion are different from each other, Wherein the upper and lower surfaces of the first and second discharge ports are formed to have the same length, and the upper surface of the selective discharge portion has a through hole passing through the second inner inlet portion at the center thereof, A side discharge hole connected to the selected passage is formed on the side surface and a lower end portion of the side surface is coupled to a coupling groove formed on the upper surface of the circular plate, the selective discharge portion is tightly coupled to the coupling groove, Thereby selecting the passage.

And a filtration screen capable of holding undigested tissue or a collagen mass among the liquid cells injected through the injection tube is further installed inside the lower body part under the injection tube.

The lower plate portion is integrally formed at the lower ends of the first inner inlet portion and the second inner inlet portion and the lower ends of the first inner inlet portion and the second inner inlet portion and has a slope equal to the slope of the upper plate portion, A circular plate part integrally formed at an end of the circular plate and having a plurality of cylindrical vanes formed at an inner side thereof and a circular plate part surrounding the outer side of the second inner inlet part of the lower plate part, And a hollow flat circular plate-shaped selective discharge portion for selecting at least one cylindrical wing and connecting the passage inside the selected cylindrical wing to the second discharge pipe, wherein the upper surface of the cylindrical wings is bonded to the upper plate portion, So that the blood component layer formed therein can be separated by the centrifugal force of the second chamber to collect the component blood A top discharge hole communicating with the second discharge pipe at both sides of the through hole is formed at an upper portion of the selective discharge portion and a side discharge hole communicating with the selected discharge passage is formed at a side of the selective discharge portion, And a lower end of the side surface is coupled to a coupling groove formed on an upper surface of the circular plate. The selection and discharge unit is tightly coupled to the coupling groove and then rotated to select the cylindrical vane.

The lower plate portion is integrally formed at the lower ends of the first inner inlet portion and the second inner inlet portion and the lower ends of the first inner inlet portion and the second inner inlet portion and has a slope equal to the slope of the upper plate portion, And a circular plate part having a circular plate spread, an end of the circular plate, and a plurality of cylindrical vanes, one end of which is connected to the inner wall surface of the second chamber and the passage is formed inside and the other end is weight- The wings are made of an elastic material, and the weights mounted on the respective vanes have different weights or weights to balance the vanes, and the weight of the wings is determined according to the centrifugal force due to the rotation of the second chamber The cylindrical vanes are opened in the direction of the inner wall surface of the second chamber and component blood can be collected according to the blood component layer.

The circular plate portion has two symmetrical cylindrical blades, and the weight mounted on the cylindrical blades has the same weight.

The transfer unit includes a first multiway valve connected to the plurality of bags, a first multiway valve connected to the first unit and the second unit, and a second multiway valve connected to the first multiway valve, And a pump connecting the second multiway valve.

The transfer unit includes a plurality of solenoid valves connected to the plurality of bags instead of the first and second multiway valves, and a transfer pipe controlled by the solenoid valves is connected to the pump.

The transferring unit is inserted with a transfer filter which can remove foreign matter such as undegraded enzyme masses or collagen masses contained in the liquid cells transferred from the first unit to the second unit. The transport filter is a doubly-fed filter.

The first unit and the second unit are each equipped with jigs to be rotated at a constant temperature, and the temperature and rotational speed of the first and second units can be adjusted.

As described above, the regenerative cell extracting system according to the embodiment of the present invention enables complete automation controlled by a program from the collection of adipose tissue to the extraction of regenerated cells, and the adipose tissue is moved and separated in a closed state, It is advantageous to selectively obtain fat tissue, mature fat cells, oil (fat), and fat-derived stem cells at each step in the separation of adipose tissue, and even when large volume such as breast enlargement is desired, It is very convenient to obtain many pure fat and fat-derived stem cells. In addition, by selectively changing the structure of the lower plate in the second chamber, it is possible to selectively separate necessary components from the blood. In this case, the desired tissue can be continuously separated even if the volume of the second chamber is large .

In addition, by automating both the rotational speed and other operations of the first unit and the second unit by a program, it is possible to automatically perform a predetermined time and capacity from the extraction of the tissue to the collection and storage of stem cells. In addition, by attaching a filter to the transfer part, various cells including stem cells can be separated from various tissues.

In order to fully understand the present invention, operational advantages of the present invention, and objects achieved by the practice of the present invention, reference should be made to the accompanying drawings and the accompanying drawings which illustrate preferred embodiments of the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the preferred embodiments of the present invention with reference to the accompanying drawings. Like reference symbols in the drawings denote like elements.

1 is a conceptual diagram illustrating a regenerative cell extraction system according to an embodiment of the present invention.

Referring to FIG. 1, a regenerative cell extracting system 10 according to an embodiment of the present invention includes a first unit 100, a second unit 200, and a transfer unit 700.

The first unit 100 collects tissue by vacuum inhalation, separates blood contaminants from the tissue harvested by stirring and centrifuging, separates the cells from the isolated tissue, Of cells.

The second unit 200 is smaller in capacity than the first unit 100 in that it is charged with the cells suspended in the liquid from the first unit 100 and separates the regenerative cells by centrifugation.

That is, the first unit 100 has a larger capacity than the second unit 200. When treating a small amount of fat (for example, less than about 100 cc), such as face molding, the second unit 200 alone can extract the necessary stem cells from the fat. However, the first unit 100 is necessary for the treatment of a large amount of fat so that the second unit 200 is difficult to handle alone. For example, when processing a tissue of about 1 liter, the tissue is concentrated by the first unit 100 into a small amount, and then transferred to the second unit 200 to extract the stem cells.

That is, the first unit 100 concentrates the cells in a large amount of fat cells and transmits them to the second unit 200.

The transfer unit 700 is connected between the first unit 100 and the second unit 200 and injects washing water into the first unit 100 and the second unit 200, The extracts from the second unit 200 are transferred to the corresponding bags B 1 and B 2 -B n and the cells suspended in the liquid separated from the first unit 100 are introduced into the second unit 200 Or transport tissue and cellular contaminants. Here, the tissue is an adipose tissue, and the regenerating cell is an adipose-derived stem cell.

Although many regenerative cell extraction devices have been developed for collecting cells from adipose tissue, as described in the prior art, these devices do not adequately accommodate a suction device for collection of adipose tissue, It is not fully automated until the processing of the tissue, and does not have a complete sealing system. Also, the system in the process of concentrating the desired cells is different from the present invention.

The regenerative cell extracting system 10 according to the embodiment of the present invention shown in FIG. 1 solves the above problems. In other words, it is possible to perform complete automation controlled by a program from the collection of adipose tissue to the extraction of regenerated cells, and also to move and separate the adipose tissue in a closed state, and to separate the adipose tissue Tissue, pure mature fat cells, oil (derived from adipocytes) and adipose-derived stem cells, respectively.

That is, the regenerative cell extracting system 10 can perform the operations of extraction of fat-derived stem cells, recovery of pure adipose tissue from which blood-derived contaminants have been removed, extraction of pure mature adipocytes and extraction of oil (derived from adipocytes) The unit 100 preferably has a capacity larger than that of the second unit 200, and the fat tissue can be taken directly from the human body by vacuum suction. This can be done by connecting the liposuction device directly to the first unit 100.

Therefore, the risk of contamination is low and the work process is simple, compared with a case where a tissue collected from a human body is once put in a separate bag and then transferred to a regenerative cell extracting apparatus.

The transfer unit 700 between the first unit 100 and the second unit 200 is connected to the first unit 100 and the second unit 200 by a pump and a valve, (B 1, B 2 -B n) are extracted from the first unit (100) by extracting cells suspended in a liquid from which blood contamination has been removed from the first unit (100) Derived stem cells from the second unit 200 and extracts the adipose-derived stem cells separated from the second unit 200 and transfers the adipose stem cells to the separate bags B1 and B2-Bn .

When the transfer part 700 is constructed using a pump and a valve, a multi-way valve can be used as the valve. The multi-way valve has a flat, round disc shape, and a plurality of passages are formed along the circumferential side surface of the disc, and a rotator capable of selecting one of the plurality of passages is formed at the center. The passages may be connected to a corresponding plurality of bags (B1, B2-Bn), respectively, and the material is moved and stored in the bag connected to the selected path through the rotator. The upper surface of the rotating body is formed with a connecting hole connected to the rotating body of another multiway valve.

The transfer unit 700 includes a first multiway valve (not shown) connected to the plurality of bags B1 and B2-Bn and a second multiway valve connected to the first unit 100 and the second unit 200, (Not shown), and a pump (not shown) connecting the first multiway valve and the second multiway valve. The second multiway valve is connected to the central pipe 160 of the first unit 100 and the inlet pipe 270 of the second unit 200.

The first multi-way valve may use the same number of passages as the number of bags (B1, B2-Bn), and the number of passages of the second multi-way valve may be equal to or less than the number of passages of the first multi- have. The pump is disposed between the first multi-way valve and the second multi-way valve and allows material to move between the first multi-way valve and the second multi-way valve. A peristertic pump may be used as the pump.

The transfer unit 700 includes a plurality of transfer pipes (not shown) and solenoid valves (not shown) connected to the plurality of bags B 1 and B 2 -B n instead of the first and second multiway valves, The solenoid valves may be structured to control a transfer pipe connected to a pump (not shown). That is, the plurality of bags (B1, B2-Bn) can be connected to the first unit 100 or the second unit 200 through the transfer pipe, and the transfer unit can be opened and closed by the solenoid valve, May be stored in the connected bags B1, B2-Bn.

The structure and operation of the transfer unit 700 can be understood by those skilled in the art, and therefore, detailed description thereof will be omitted.

2 (a) is a cross-sectional view for explaining the structure of the first unit.

Referring to FIG. 2 (a), the first unit 100 includes a first chamber 110 and a central tube 160.

The first chamber 110 is a cylindrical portion having a lower portion sloped and taped to remove blood contaminants from the tissue and separate the cells.

The first chamber 110 may be configured to perform aspiration of adipose tissue, removal of blood-derived contaminants, dehydration of pure adipose tissue, degradation using an enzyme (usually using collagenase but using other enzymes alone or in combination) Operation is performed.

The first chamber 110 includes a cylindrical upper body part 120 and a middle body part 130 integrally formed at the lower part of the upper body part 120 and inclined downward toward the center, A lower body part 140 formed integrally with the middle body part 130 and extending downwardly and having an inner diameter smaller than an inner diameter of the upper body part 120 and having the same inner diameter, And a lid 150 formed to cover the upper surface. The shape of the bottom surface of the lower body part 140 may vary, but it is preferable that the bottom surface part of the lower body part 140 protrudes slightly in a U-shape as shown in FIG.

The size of the first chamber 110 is about 120 mm in diameter and about 170 mm in height and larger than that of the second chamber 210 of the second unit 200, .

That is, when the capacity is less than 1,000 cc, the height of the first chamber 110 may be about 50 mm, the height may be about 150 mm up to 1,000 - 2,000 cc, and the length may be about 250 mm up to 2,000 - 3,000 cc. However, this is only an example, and the size can be variously determined.

The central tube 160 is inserted through a guide hole 151 formed at the center of the lid 150 to a position spaced apart from the bottom surface of the lower body tube 140 by a predetermined distance. Preferably, the lower end of the central tube 160 is spaced apart from the bottom surface of the lower body part 140 by a distance of 0.1 mm to 3 mm from the bottom surface.

Tissue is introduced into the first chamber 110 through the upper portion of the central tube 160, and the cells are discharged from the first chamber 110. A cap is provided at the top of the central tube 160. The cap has a structure (luer lok structure) as seen in a safety syringe and is directly connected to the liposuction device, Allowing the fat sucked in by the negative pressure to be directly introduced into the first chamber 110 and being sealed until it is mounted to the fat cell separator after inhaling the fat.

A transfer bag is connected to the top of the central tube 160 and a separate vacuum pump connected to the negative pressure pipe 190 (most of which is connected to the first vacuum pump) The fat tissue may be transferred to the first chamber 100 by the negative pressure induced by the fluid (depending on the fat sorter) or may be transferred into the first chamber 100 using a pump built into the regenerative cell extractor of the present invention .

The first chamber 110 includes a sound-pressure tube 190 which surrounds the central tube 160 and is inserted into the guide hole 151 and is integrally formed with the central tube 160 and is used to turn the first chamber 110 into a vacuum sound pressure state. The first chamber 110 is rotated independently of the center pipe 160 and the sound pressure pipe 190 to maintain the hermeticity between the wall surface 153 forming the guide hole 151 and the sound pressure pipe 190 A retainer 180 is mounted.

The retainer is a component that functions to assist rotation such as a bearing, but also to seal air so that it does not pass through. It is natural that elements other than the retainer can be used.

A bearing (not shown) for smoothly rotating the first chamber 110 may be mounted between the wall 153 and the sound pressure pipe 190 forming the guide hole 151, together with a retainer have. In addition, a filter (not shown) capable of filtering out foreign substances existing in the tissues introduced through the central tube, that is, substances other than fatty tissue, such as collagen masses and the like, The lower body 140 can be mounted on the lower portion of the lower body 140.

The sound-pressure portion 190 is equipped with a filter 191 for preventing backflow of contaminated air. The sound-pressure portion 190 is a portion that applies a negative pressure to collect the adipose tissue in the operating room, that is, a portion that can be connected to the vacuum inhaler. Thus, the stopper and the plug- Do. The type of filter 191 may vary and may be a 0.22 um air filter to prevent backflow of contaminated air in a vacuum inhaler.

At least one first wing portion 170 is formed in the inner wall surface of the middle body portion 130 of the first chamber 110 in the direction of the center pipe 160 in order to smooth agitation of the collected tissue. The first wing portion 170 is preferably mounted at four degrees at an angle of 90 degrees within the first chamber 110, with a thickness of about 1-2 mm. The first wing portion 170 allows the fat tissue and washing water to mix well when the first chamber 110 repeats rotation and stopping.

 In addition, the central tube 160 may include at least one auxiliary vane 175 protruding outward at a portion inserted into the first chamber 110. The auxiliary wing (175) also ensures that the fat tissue and wash water are well mixed when the first chamber (110) repeats rotation and stop.

The first unit 100 is mounted on a jig (not shown) and rotates at a constant speed and temperature. The jig can control the temperature and the rotation speed of the first unit 100, and the structure and operation of the jig can be understood by those skilled in the art, so that detailed description will be omitted.

In addition, the first unit 100 may be mounted on the jig using the eccentric motor so that the first unit 100 performs only the function of stirring by using vibration instead of rotation.

The operation of the first unit 100 will be described below.

First, it absorbs fat tissue from the body (Fat tissue aspiration)

The cap on the upper portion of the central tube 160 is removed and the liposuction needle of the liposuction device is connected to the upper portion of the central tube 160. Since the liposuction needle is used for general purpose, the upper portion of the central tube 160 can be coupled to the liposuction device, so that the liposuction needle can easily be connected to the upper portion of the central tube 160.

The lid of the upper portion of the sound pressure portion 190 is opened and the vacuum inhaler is connected. Then, the vacuum inhaler is operated to collect fat tissue directly from the body. The collected fat tissue contains blood and the like. The regenerative cell extracting system 10 of the present invention is a system in which the liposuction device and the vacuum inhaler are directly connected to the regenerative cell extracting system in which the regenerative cell extracting system of the present invention extracts adipose tissue from the body, By connecting to the first unit 100, fat tissue can be directly collected from the body and accommodated in the first chamber 110. Therefore, contamination of the adipose tissue, infection, storage problems, etc. can be solved at once.

After collecting adipose tissue, remove the connection tube between the needle and the vacuum inhaler. At this time, the cap on the connection side of the vacuum inhaler is left unopened for a while. That is, the connection to the vacuum inhaler is removed first, and the inside of the first chamber 110 is restored to the atmospheric pressure. Then, the connection part of the collection needle of the main tube 160 is removed to prevent the contaminated air from flowing into the main tube 160 .

The vacuum tube 190 connected to the vacuum inhaler has a filter 191 so that bacteria may not be contaminated by outside air even if the cap is opened

A kit for an adipose-derived stem cell of the transfer part 700 is connected to an upper part of a central tube 160 to which an adipose tissue drawing needle is connected. In this kit, various bags (B1, B2 ~ Bn) are connected as in the cord blood treatment kit, and each bag is equipped with a solenoid valve. Normally, a transfer tube connected to the solenoid valve is pressed to prevent the bag from being pushed, A collagenase, a bag for storing fat tissue from which blood has been removed, a bag for collecting discarded liquid after the treatment, and a bag connected to the second unit 200, and the like, . Also, as described above, instead of a solenoid valve, a transfer pipe and a pump, a kit constructed using a multiway valve and a pump may be connected to the transfer unit 700. In this case, a solenoid valve in front of the bag is necessary no.

Second, the blood contaminants are removed (pure fatty tissue separation).

The first unit 100 is mounted on a rotary and temperature adjustable jig having an inner diameter equal to the outer diameter of the first unit 100. [ Of course, an operation of absorbing adipose tissue may be performed while being mounted from the beginning.

The first unit 100 and the second unit 200 can be mounted on rotating jigs, respectively, while maintaining a constant temperature. The jigs (not shown) are capable of adjusting the temperature and rotational speed of the mounted first unit 100 and the second unit 200.

The first unit 100 is left to stand for about one minute to separate the fatty tissue and water from the absorbed fat tissue. Then, the fatty tissue is located above the water inside the first chamber 110. After the pump of the transfer unit 700 is connected to the tube of the kit, the pump is operated so that water contaminated with blood that has settled in the lower part of the first chamber 110 is inhaled through the central tube 160 and removed. At this time, the sensor is attached to the connection pipe between the pump and the bag which receives the water contaminated with the blood, and the pump is automatically stopped when all the water including the blood passes. Unlike adipose tissue, the color of blood, including blood, becomes red due to blood or is very dense compared to adipose tissue, so the sensor senses the color (or brightness or density or permeability) of the material passing through the tube, And the pump operation can be controlled by distinguishing the non-reacting substances.

(Weight) of the substance including blood and the specific gravity (weight) of the fat tissue are different, so that the operation of the pump can be controlled by sensing the difference in specific gravity. Since the operation of such a sensor can be understood by those skilled in the art, a detailed description will be omitted.

A cleaning water (saline solution) bag is opened and an amount of washing water similar to that of the fatty tissue remaining in the first chamber 110 of the first unit 100 is introduced by the operation of the pump. It is possible to set the amount of washing water to be introduced by the program in advance according to the amount of the collected fat tissue. Then, the first chamber 110 is rotated. The rotational speed or frequency can also be set programmatically by the amount of fat tissue sampled. For example, it can be adjusted by repeating 10 seconds of rotation with about 300 revolutions per minute, 5 times with 10 seconds of stoppage, and so on.

In this manner, the operation of washing the adipose tissue with the saline solution, removing the washed portion of the water again, and washing with the saline solution is repeated 3-5 times. Finally, the saline solution is removed. Only the fat tissue necessary for separating the fat-derived stem cells is left in the first chamber 110, and the remaining washed fat tissue is transferred to a bag for storing pure fat using a pump. That is, the regenerative cell extracting system 10 according to the embodiment of the present invention can separately separate only the pure adipose tissue that has been washed as needed.

Third, the cells are separated using enzymes.

A similar amount of enzyme (0.1% collagenase, as used in the washed fat cells) remains in the first chamber 110. However, one skilled in the art will appreciate that other enzymes may be used and concentrations may vary. And is introduced into the first chamber 110 through the center pipe 160 by using a pump. At this time, since the amount is expected in advance, the amount of the enzyme can be initially set in the program.

After the enzyme is added, the rotation and stop of the first chamber 110 are repeated according to the program. At this time, heat is applied to the outside of the first chamber 110 to maintain the temperature at about 37 degrees. This is done by the heat transferred from the jig and is to activate the enzyme.

When the rotation and stopping are repeated for a predetermined time (for example, about 30 minutes), the mature fat cells and fat-derived cells are decomposed by the enzyme in the first chamber 110, When stopped, the fluid layer (lower layer) containing mature adipocytes (upper layer) and adipose derived cells is separated. Then, as a result of the treatment with the collagenase, the fluid layer containing the separated fat-derived cells is moved to the second chamber 210 of the second unit 200 by using a pump. If the mature adipocytes and mature adipocytes are in need of liquid fats (oil) formed by the ingestion or treatment, the enzyme (collagenase) in the first chamber 110 is treated with the 3- After 5 washes, transfer to a bag for storing mature fat cells using a pump. This process is also possible initially by program settings and can be controlled by sensors.

As described above, the first unit 100 can be directly extracted from the adipose tissue, extracted from the pure adipose tissue, extracted mature adipocytes using the enzyme, and separated for separating the stem cells. Conditions may be set in advance by the program so that only the whole process or necessary process can be performed separately.

Fig. 2 (b) is a view showing another structure of the first unit of Fig. 2 (a).

2 (b), the sound-pressure portion 190 may be mounted directly to the cover portion 150 in a state of being coupled thereto. The sound-pressure unit 190 may be provided with a filter 191 for preventing backward flow of polluted air.

3 is a cross-sectional view illustrating the structure of the second unit.

4 is a plan view for explaining the structure of the lower plate portion of Fig.

3 and 4, the second unit 200 includes a second chamber 210, a lower plate 264, an inlet pipe 270, a first outlet pipe 275, and a second outlet pipe 280 do.

The second chamber 210 has an inlet part 220 having a predetermined diameter and extending vertically downward and a cell separating part 225 having an inner diameter larger than the lower end of the inlet part 220 and having a maximum inner diameter, And a lower body portion 240 integrally formed at a lower portion of the upper body portion 230 and extending downward with a constant inner diameter. The upper body portion 230 has a cylindrical shape. The bottom surface of the lower body portion 240 may be flat or a structure in which the bottom surface protrudes in a U-shape as shown in FIG.

The second chamber 210 is inclined toward the center as it goes down from the cell separation part 225 of the upper body part 230. This is because when the second chamber 210 rotates, ), The separated fat-derived stem cells can be collected in the cell separator 225 when the liquid containing the adipose-derived stem cells is introduced and separated.

In addition, when the liquid containing the adipose-derived stem cells continuously flows from the first unit 100, separation of the adipose-derived stem cells from the wall portion far from the inlet, which is the lower end of the injection tube 270, So that when the liquid solutions in which the adipose-derived stem cells are removed are removed into the upper outlet, the cells can not easily escape.

The lower plate portion 264 is provided so as to correspond to the inner surface of the upper plate portion 250 when the portion from the lower end of the inlet portion 220 toward the lower portion toward the cell separating portion 225 is referred to as an upper plate portion 250 A physiological buffer solution other than the regenerative cells present inside the second chamber 210 is discharged along the passage 265 between the upper plate 250 and the lower plate 264. [

The lower plate portion 264 is integrally connected to the upper end of the lower plate portion 264 and surrounds the cylindrical first inner inlet portion 261 having the first inner diameter and the first inner inlet portion 261, A second inner inlet portion 263 having a larger second inner diameter extends inward of the inlet portion 220.

The inlet pipe 270 is inserted into the second chamber 210 through the first inner inlet portion 261 to inject the cells discharged from the first unit 100 into the second chamber 210.

The first outlet pipe 275 is inserted into the second chamber 210 through the inlet pipe 270 and longer than the inlet pipe 270 and is connected to the bottom of the lower body 240 of the second chamber 210, And the regenerative cells formed on the surface are discharged.

Preferably, the inlet tube 270 is inserted to the lower end of the upper body portion 230 and the first outlet tube 275 is inserted to a height of 0.1 to 2 mm from the bottom surface of the lower body portion 240.

The upper portion of the first discharge pipe 275 has a shape in which a safety syringe can be attached to discharge the recovered fat-derived stem cells to the outside, and is plugged with a plug or connected to a kit for performing the process. Since the end of the first discharge tube 275 is lowered to a height of 0.1 to 2 mm from the bottom surface of the lower body 240, the adipose-derived stem cells accumulated on the lower surface of the lower body 240 are discharged through the first discharge tube 275, As shown in FIG. An inlet tube 270 surrounding the first outlet tube 275 and used for transporting the cells from the first unit 100 to the second unit 200 is installed.

The second discharge pipe 280 is inserted between the outer wall surface of the second inner inlet portion 263 and the inner wall surface of the inlet portion 220 and is discharged through the passage 265 between the upper plate portion 250 and the lower plate portion 264 The physiological buffer solution other than the regenerative cells is discharged to the outside.

The second chamber 210 maintains tightness while rotating independently of the inlet pipe 270, the first outlet pipe 275 and the second outlet pipe 280 by retainers 281 and 283.

In the second unit 200, the fat-derived stem cells contained in the fluid including the enzyme transferred from the first unit 100 are washed and the fat-derived stem cells are separated.

The inlet tube 270 has a first support vane 271 surrounding the outer wall of the first inner inlet 261 and a second outlet vane 280 having a second support vane 271 surrounding the inlet 220, And a support vane 285. Both the first support vane 271 and the second support vane 285 rotate independently of the second chamber 210, the first outlet pipe 275 and the second outlet pipe 280, 281, 283) to be hermetically sealed.

That is, between the inner wall surface of the first support vane 271 and the second inner inlet portion 263, between the outer wall surface of the inlet portion 220 and the second support vane 281, the second chamber 210 A retainer is mounted to maintain airtightness while rotating independently.

The retainer is an element that functions to assist rotation such as a bearing while sealing the air so that it does not pass through. It is a matter of course that an element performing such a function may be used in addition to the retainer, A bearing that smoothly rotates the two chambers 210 can be mounted at the same time.

The second chamber 210 may be configured such that when the second chamber 210 is rotated on the inner wall surface from the cell separator 225 to the lower end of the upper body 230, At least one second wing portion 211 is formed in the direction of the injection tube 270. Preferably, as in the first chamber 110, four second wing portions 211 may be disposed at 90 degree intervals, and as in 175 of the first chamber 110, A second wing portion (not shown) may be mounted.

The lower plate portion 264 is composed of a first inner inlet portion 261, a second inner inlet portion 263, and a circular plate 260. The circular plate 260 is integrally formed at the lower ends of the first inner inlet portion 261 and the second inner inlet portion 263 and spreads like a lance having the same slope as the slope of the upper plate portion 250, Thereby forming a passage 265 together.

At least one passage 265 is provided between the upper plate 250 and the lower plate 264 and the portion 410 of the circular plate 260 between the passage and the passage is bonded to the upper plate 250.

4, a certain portion 410 of the circular plate 260 of the lower plate portion 264 is adhered to the upper plate portion 250. As shown in FIG. Between the portion 410 and the portion 410 adhered to the upper plate 250 is separated from the upper plate 250 to thereby form the passage 265 naturally. 3, the passage 265 is marked for easy understanding. As the second chamber 210 rotates, the passage 265 moves the cells inside the second chamber 210 and the washing water to the cell separator 225, which is the outermost portion of the second chamber 210, The stem cells are arranged close to the wall surface of the cell separator 225 and the cleansing water is disposed away from the wall of the cell separator 225 rather than the stem cell. The washing water is drained through the passage 265 by applying a negative pressure or applying a positive pressure to the inside of the second chamber 210 and performing both of the same at the same time so that only a small amount of liquid and stem cells remain in the second chamber 210 Let's do it.

By adjusting the rotation speed of the second chamber 210 and the length of the circular plate 260, it is possible to collect the blood component according to the blood component layer formed inside by the centrifugal force of the second chamber 210, The amount or rate of release of the physiological buffer solution other than the regenerative cells through the passage 265 can be controlled.

A filtration net 213 capable of filtering the remaining undegraded tissue including enzyme lumps or a substance such as a collagen mass among the liquid cells injected through the inlet tube 270 is supplied to the lower body portion 240 As shown in Fig. The mesh 213 is a mesh network having a gap of about 0.5-2 mm which is completely disintegrated when cells are introduced into the second unit 200 through the inlet tube 270 in the first unit 100. [ Such as a portion of a collagen mass or tissue that has not been degraded by enzymes used in the first unit 100.

The prevention film 215 may be installed at the entrance of the passage 265 so that the regenerative cells gathered in the cell separation unit 225 during the rotation of the second chamber 210 may escape only through the passage 265, have. 3, the prevention film 215 may be disposed in a portion of the passage 265 between the circular plate 260 and the inner wall surface of the upper plate 250, similar to the structure of the lower plate 264. [ At this time, the prevention film 215 may have a structure in which a hole is formed at the center as shown in FIG. 4, and a part thereof is bonded to the inner wall surface of the upper plate 250 like the portion 410 in FIG.

Hereinafter, the operation of the second unit 200 will be described.

First, an enzyme contained in the sap containing the regenerative cells transferred from the first unit 100 (a physiological buffer containing various culture media, etc.) is washed and the volume of the solution is reduced, so that the volume reduction ) Process is performed.

The saline solution containing the collagenase and the cells from the first unit 100 is gradually filled into the second chamber 210 of the second unit 200 through the inlet tube 270. The second chamber 210 is initially stopped and rotates the second chamber 210 when a predetermined amount is filled in the second chamber 210. For example, when the fat cells mixed with the collagenase are filled with half the total volume of the second chamber 210, the second chamber 210 is rotated to about 150G. (The G value can be changed to 75 G to 1,000 G or more, and when the G value is large, the concentration time is shortened.) The rotation start timing of the second chamber 210 is set so that the amount of liquid transferred per revolution of the pump is constant Therefore, it is possible to calculate the amount transferred by the time calculation, and these conditions are programmed so that the rotation can be started automatically.

Similarly to the first chamber 110, the second chamber 210 is mounted on a jig (not shown) and rotates at a constant speed and temperature. In this way, by controlling the first unit 100 and the second unit 200, the rotation time, the temperature, the rotation speed, and the like are set by a program in advance, and the whole process from the extraction of the fat tissue to the separation of the fat- can do.

Such programs and automation can be understood by those skilled in the art and will not be described in detail.

Simultaneously with the start of rotation of the second chamber 210, the physiological buffer solution including the cells coming from the first unit 100 is blocked for about 5 minutes to prevent cell loss. When the flow of the physiological buffer including the cells transferred from the first unit 100 simultaneously from the beginning of the rotation and the flow of the physiological buffer separated from the regenerative cells due to the rotation of the second chamber 210 proceed together, The regenerative cells contained in the physiological buffer may be partially effluxed before separation to greatly reduce the recovery rate of the regenerative cells. Therefore, after the second chamber 210 is filled with a predetermined amount or more, the cells are rotated for about 5 minutes to completely separate the regenerated cells from the physiological buffer solution, and then the inflow and outflow of the physiological buffer solution including the cells from the first unit 100 Lt; RTI ID = 0.0 > regeneration < / RTI >

After about 5 minutes, the second chamber 210 continuously delivers the physiological buffer solution containing the collagenase and cellular components remaining in the first unit 100, and at the same time, continuously supplies the liquid component ( Cleaning water) is removed from the second chamber 210. As the liquid component in the second chamber 210 is removed simultaneously with the rotation, the adipose-derived stem cells are collected in the cell separation unit 225 of the second chamber 210. When the liquid component is continuously extracted, Only the liquid in the second chamber 210 remains. At this time, both the flow of physiological buffer and the separation and outflow of adipose-derived stem cells, the rotation speed and the harmony of inflow and outflow rates are very important to prevent the loss of regenerative cells.

As described above, the second chamber 210 is constructed such that the regenerative cells are mixed with the enzyme and the saline solution through the inlet tube 270 from the first chamber 110, and the liquid component By discharging the stem cells through the second drain pipe 280, pure stem cells can be isolated.

Second, the rotation of the second chamber 210 is stopped and the saline solution is added back to the second chamber 210. [ Then, the rotation and stopping operations of the second chamber 210 are repeated. For example, repeating about 10 seconds of rotation, repeating 10 seconds of stoppage 3-5 times, and so on. Thereafter, the second chamber 210 is rotated again to remove the saline solution. Repeat this wash 3-5 times. The rotation of the second chamber 210 is stopped after the last washing process. When the rotation of the second chamber 210 is stopped, the adipose-derived stem cells gathered on the wall surface of the cell separator 225 are guided to the bottom surface of the lower body part 240 on the inclined surface of the upper body part 230 It flows down.

Finally, the plug at the top of the first outlet tube 275 is removed, the syringe is inserted, and saline containing the desired amount or the whole amount of the fat-derived stem cells is taken out by a syringe. Since the end of the first drain pipe 275 is lowered to 0.1-2 mm from the bottom of the lower body 240, the fat-derived stem cells gathered on the bottom surface of the lower body 240 can be easily extracted to the outside.

In the case where the amount of adipose tissue collected from the body is not sufficient, the whole process is performed only with the second unit 200 without the first unit 100, and the adipose-derived stem cells can be separated. In other words, when the amount of adipose tissue is small, the second unit 200 may be used for washing the adipose tissue, separating mature adipocytes, separating the regenerative cells by injecting the enzyme, rinsing the adipose stem cells to improve purity, Volume reduction, and discharge operation of adipose-derived stem cells may be performed only in the second unit 200.

Fig. 5 is a plan view for explaining another structure of the lower plate portion of Fig. 3;

Fig. 6 (a) is a plan view of the selective discharge portion coupled to the lower plate portion of Fig. 5;

6 (b) is a side sectional view of the selective discharge portion.

6 (c) is a sectional view of the second unit for explaining a structure in which the selective discharge portion is coupled to the lower plate portion.

5 to 6, a lower plate portion 264-1 according to another embodiment of the present invention includes a first inner inlet portion 261, a second inner inlet portion 263, and a first inner inlet portion 261 and the second inner inlet portion 263 and is integrally formed with the upper plate portion 250 and has a slope equal to the slope of the upper plate portion 250. The circular plate 260 forming the passage 265 together with the upper plate portion 250 1 that surrounds the second inner inlet portion 263 of the lower plate portion 264-1 and selectively connects at least one of the passages 265-1 to the second outlet pipe 280, Shaped discharge portion 600 of a flat disk shape.

At least one passage 265-1 is provided between the upper plate 250 and the lower plate 264-1 and a circular plate portion 510 between the passages and the passage is formed in the upper plate 250, .

When separating the circular plate 260-1 in the radial direction, the separated half portions have the same weight, the lengths of the passages formed in one half portion are different from each other, Passages facing each other have the same length.

That is, as shown in FIG. 5, the circular plate 260-1 has a structure in which the passages 265-1 having different lengths from short to long are symmetrical to each other.

By making the lengths of the passages 265-1 to be different from each other, it is possible to obtain a desired substance at a necessary position when different substances are to be obtained in each layer generated as a result of centrifugation of blood or the like by rotation of the second chamber 210 have.

The reason for having a symmetrical structure is that vibrations may occur in the rotating second chamber 210 if the center of gravity does not match the center of gravity during centrifugation. In this case, if the weight of the passages 265-1 having different lengths of the circular plate 260-1 is matched so as to be symmetrical to each other, vibration does not occur even when the second chamber 210 rotates . The adjustment of the weight is a basic principle of the centrifugal separator, and a detailed description thereof will be omitted since it can be understood by those skilled in the art.

A through hole 605 through which the second inner inlet portion 263 penetrates and a through hole 605 through the center are formed on the upper surface 640 of the selective discharge portion 600 of FIGS. 6 (a) and 6 And an upper surface discharge hole 610 connected to the second discharge pipe 280 is formed. The portion of the inner wall surface 263-1 of the selective discharge portion 600 forming the through hole 605 is divided into upper and lower portions so as to be easily brought into contact with the second inner inlet portion 263, And is extended by a predetermined length.

A side discharge hole 615 connected to the selected passage 265-1 is formed on a side surface of the selective discharge portion 600 and a lower end portion 620 of the side surface is provided with a coupling groove 520 formed on the upper surface of the circular plate 260-1 ).

6 (c), the lower end portion 620 of the selective discharge portion 600 is inserted into the coupling groove 520 of the circular plate 260-1 and is tightly coupled. Then, the side discharge hole 615 of the selective discharge portion 600 is rotated so as to select the corresponding passage 265-1. The side lower end portion 620 of the selective discharge portion 600 may be closed by a sealing ring or the like using a silicone ring or the like, The passage may be selected by rotating in a state where it is coupled to the piston 520. The structure in which the selective discharge portion 600 is engaged with the coupling groove 520 and rotates will be understood by those skilled in the art, and thus a detailed description thereof will be omitted.

The first unit 100 and the second unit 200 having such a structure are mounted on a rotary jig (not shown) and rotated, and the rotational speed can be automatically adjusted by being set by a program in advance. The separation of fat-derived stem cells from such rotational speeds or adipose tissue harvesting and the storage of the separated materials into the backbone (B1-Bn) can be automated by means of programs and software, and such automation processes can be understood by those skilled in the art of software The detailed description will be omitted.

Fig. 7 is a plan view for explaining another structure of the lower plate portion of Fig. 3;

8 is a cross-sectional view illustrating a state in which the lower plate portion of Fig. 7 is mounted.

7 is a plan view of the lower plate portion 264-2 and a top view and a side view of the selective discharge portion 700 coupled to the lower plate portion 264-2.

7 and 8, the other structure of the lower plate portion 264-2 according to the embodiment of the present invention is different from the structure of the lower plate portions 264 and 264-1 of Figs. 4 and 5, A cylindrical blade cutter 750 is separately provided.

More specifically, the lower plate portion 264-2 includes a first inner inlet portion 261 and a second inner inlet portion 263, a circular plate portion 761, and a selective discharge portion 700.

The circular plate portion 761 includes a circular plate 760 integrally formed at the lower ends of the first inner inlet portion 261 and the second inner inlet portion 263 and having a slope equal to the inclination of the upper plate portion 250, And a plurality of cylindrical vanes 750 integrally formed at an end of the circular plate 760 and having a passage 265-2 formed therein.

The selective discharge portion 700 surrounds the outside of the second inner inlet portion 263 of the lower plate portion 264-2 and selects at least one cylindrical vane out of the cylindrical vanes 750, 265-2 to the second discharge pipe 280. The second discharge pipe 280 has a hollow flat plate shape.

8, the upper surfaces of the cylindrical vanes 750 are bonded to the upper plate 250, and the lengths thereof are different from each other, so that the blood component layer formed inside the chamber is separated by the centrifugal force of the second chamber 210 So that the component blood can be collected.

That is, when the second chamber 210 rotates, the substances included in the physiological buffer solution including the inner fat-derived cells form a layer according to the specific gravity by the centrifugal force. Therefore, the substances in the blood component layer can be separated and discharged according to the length of the cylindrical wings 750.

The upper surface of the selective discharge unit 700 includes a through hole 705 through which the second inner inlet portion 263 passes and a top surface discharge hole 710 connected to the second discharge pipe 280 on both sides of the through hole 705, . (See the middle diagram in Fig. 7)

A side discharge hole 715 connected to the selected passage is formed on the side of the selective discharge unit 700 and a lower end 720 of the side is coupled to the coupling groove 722 formed on the upper surface of the circular plate 760, The part 700 is tightly coupled to the engaging groove 722 and then rotated to select the cylindrical wing 750.

7, the selective discharge portion 700 has a flat disk shape having a through hole 705. The lower end portion 720 is inserted into the coupling groove 722 of the circular plate 760 and the coupling groove 722 The side discharge hole 715 can select the passage. The coupling groove 722 is provided with a device such as a silicone ring so that the coupling between the lower end 720 and the coupling groove 722 can be sealed.

8, the cylindrical vanes 750 of the lower plate portion 264-2 are adhered to the corresponding upper plate portions 250. As shown in Fig. The circular plate 760 of the lower plate portion 264-2 is formed with a coupling groove 722 along the circumference of the circular plate 760 on which the cylindrical vanes 750 are formed, 700 are coupled.

By making the length of the cylindrical wing 750 different, necessary materials can be separately stored and stored from the blood component layer.

As shown in FIG. 7, the inner wall surface 263-2 of the selective discharge portion 700 forming the through-hole 705 is formed to have a predetermined length at the upper and lower portions to facilitate contact with the second inner inlet portion 263 Respectively.

9 is a cross-sectional view illustrating a state in which the lower plate of another structure is mounted in the second chamber.

The lower plate portion has a first inner inlet portion 261 and a second inner inlet portion 263 and a circular plate portion 961. The circular plate portion 961 has a circular plate 960 integrally formed at the lower ends of the first inner inlet portion 261 and the second inner inlet portion 263 and having a slope equal to the inclination of the upper plate portion 250, One end is connected to the end of the circular plate 960 and the inner wall surface of the second chamber 210, the passage 265-3 is formed on the inner side, and the other end has a different weight for balancing the cylindrical wing And a plurality of cylindrical vanes 950 on which a weight (not shown) having the same weight is mounted.

9, cylindrical vanes 950 are formed at the ends of the circular plate 960 in the same manner as the lower plate 264-2 of FIG. 7, but the lower plate 264-2 The cylindrical wings 750 may be fixed to the upper plate 250 and have a plurality of different lengths. However, in another embodiment, only two cylindrical wings 950 may be present. The cylindrical wings 950 are made of a resilient material, and the weight weight (not shown) attached to each of the cylindrical wings may have different weights or the same weight to balance the cylindrical wings with each other .

If there are two cylindrical wings, the weight attached to the cylindrical wing should have the same weight.

When the second chamber 210 is stopped, the cylindrical wings 950 are downwardly moved by a weight (not shown) as shown in FIG. 9, and when the second chamber 210 rotates, The cylindrical wings 950 are spread toward the inner wall surface of the second chamber 210 according to the centrifugal force and component blood can be collected according to the blood component layer.

Since the centrifugal force acts on the outside of the second chamber 210, the cylindrical wings 950 are unfolded toward the inner wall surface of the second chamber 210 according to the rotation speed of the second chamber 210. If the rotation speed is high, the inner wall surface of the second chamber 210 is very close to the inner wall surface of the second chamber 210, and if the rotation speed is slow, the second chamber 210 is far away from the inner wall surface. With such a principle, the cylindrical wings 950 can selectively discharge the substances of the required layer in the blood component layer formed inside the second chamber 210.

The cylindrical wing 950 is connected to the end of the circular plate 960 and also fixed to the inner wall surface of the second chamber 210. 9, the portion opposite to the connecting portion of the circular plate 960 of the cylindrical vane 950 is connected to the inner wall surface of the chamber 2 of the ash chamber. To ensure the fixing, one side of the cylindrical vane 950 So that the end portion is caught and fixed to the latching jaw 970 formed on the inner wall surface.

There are various ways in which the cylindrical wing 950 is fixed to the inner wall surface of the second chamber 210, and a detailed description thereof will be omitted since it can be understood by those skilled in the art.

The transfer unit 700 may include a transfer filter (not shown) for inserting foreign materials such as undigested collagen masses contained in liquid cells transferred from the first unit 100 to the second unit 200 have. The transfer filter may be a dual filter. The duplex filter has a structure in which a filter having a small hole size formed in a mesh net is surrounded by large holes formed in a mesh net. Specifically, the duplex filter may have a structure in which a 0.5 mm to 2 mm filter and a 0.1 mm to 0.5 mm filter are sequentially mounted on the transfer unit 700. In yet another embodiment, the transfer filter may be a single filter rather than a dual filter.

 As described above, various cells including stem cells can be isolated from various tissues other than adipose derived stem cells by the filter mounted on the transfer unit 700.

As described above, an optimal embodiment has been disclosed in the drawings and specification. Although specific terms have been employed herein, they are used for purposes of illustration only and are not intended to limit the scope of the invention as defined in the claims or the claims. Therefore, those skilled in the art will appreciate that various modifications and equivalent embodiments are possible without departing from the scope of the present invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

BRIEF DESCRIPTION OF THE DRAWINGS A brief description of each drawing is provided to more fully understand the drawings recited in the description of the invention.

1 is a conceptual diagram illustrating a regenerative cell extraction system according to an embodiment of the present invention.

2 (a) is a cross-sectional view for explaining the structure of the first unit.

Fig. 2 (b) is a view showing another structure of the first unit of Fig. 2 (a).

3 is a cross-sectional view illustrating the structure of the second unit.

4 is a plan view for explaining the structure of the lower plate portion of Fig.

Fig. 5 is a plan view for explaining another structure of the lower plate portion of Fig. 3;

Fig. 6 (a) is a plan view of the selective discharge portion coupled to the lower plate portion of Fig. 5;

6 (b) is a side sectional view of the selective discharge portion.

6 (c) is a sectional view of the second unit for explaining a structure in which the selective discharge portion is coupled to the lower plate portion.

Fig. 7 is a plan view for explaining another structure of the lower plate portion of Fig. 3;

8 is a cross-sectional view illustrating a state in which the lower plate portion of Fig. 7 is mounted.

9 is a cross-sectional view illustrating a state in which the lower plate of another structure is mounted in the second chamber.

Claims (24)

A first unit for collecting the tissue by vacuum suction, separating blood contaminants from the tissue collected by stirring, and separating the cells from the separated tissue of the blood contaminants and discharging cells suspended in the liquid; A second unit having a capacity smaller than that of the first unit, the second unit being charged with cells suspended in the liquid from the first unit and separating the regenerative cells by centrifugation; And Wherein the first and second units are connected between the first unit and the second unit and inject wash water into the first unit and the second unit and deliver the extract from the first unit and the second unit to the corresponding bags, And a transfer unit for transferring cells suspended in the liquid extracted from the first unit to the second unit or transferring tissue and cell contaminants, The second unit comprising: A cylindrical upper body portion having an inner diameter which gradually decreases from the lower end of the inlet portion toward the lower portion of the cell separating portion having a larger inner diameter and a larger inner diameter than the lower portion of the inlet portion, A second chamber formed integrally with a lower portion of the body and having a lower body extending downward with a predetermined inner diameter; The upper part of the upper part of the upper part is connected to the inner surface of the upper part of the cell separation part. A cylindrical inner first inlet portion having a first inner diameter formed inside the inlet portion and a second inner diameter larger than the first inner diameter and surrounding the first inner inlet portion; A lower plate portion having a second inner inlet portion; An inlet tube that is inserted into the second chamber through the first inner inlet portion to inject cells suspended in the liquid discharged from the first unit into the second chamber; A first discharge pipe inserted into the second chamber through the inner side of the injection tube and longer than the injection tube and serving as a passage for discharging the regenerative cells formed on the bottom surface of the lower body of the second chamber; And And a second discharge pipe which is inserted between an outer wall surface of the second inner inlet portion and an inner wall surface of the inlet portion and discharges the physiological buffer solution other than the regenerative cells discharged through a path between the upper plate portion and the lower plate portion to the outside, Wherein the second chamber is rotated independently of the inlet tube, the first outlet tube, and the second outlet tube by a retainer to maintain the hermeticity. delete delete delete delete delete delete delete The method according to claim 1, The inlet tube having a first support vane surrounding the outer wall surface of the first inner inlet section on the outside and a second support vane surrounding the inlet section, The retainer is mounted between the first support vane and the inner wall surface of the second inner inlet portion and between the outer wall surface of the inlet portion and the second support vane to maintain airtightness while the second chamber rotates independently Wherein the regenerating cell extracting system comprises: 10. The method of claim 9, A bearing is further provided between the first support vane and the inner wall surface of the second inner inlet portion and between the outer wall surface of the inlet portion and the second support vane to smooth the rotation of the second chamber together with the retainer Wherein the regenerating cell extracting system comprises: The apparatus according to claim 1, And the first discharge pipe is inserted from the bottom surface of the lower body part to a height of 0.1 mm to 2 mm. The apparatus of claim 1, wherein the second chamber comprises: Wherein at least one second wing portion for smoothly stirring the second chamber when the second chamber rotates is formed in the inner wall surface from the cell separation portion to the lower end of the upper body portion in the direction of the injection tube. The apparatus according to claim 1, The first inner inlet portion and the second inner inlet portion, And a circular plate integrally formed at a lower end of the first inner inlet portion and the second inner inlet portion and having a slope equal to the slope of the upper plate portion and extending in a lance shape and forming the passage together with the upper plate portion, Wherein at least one passage exists between the upper plate portion and the lower plate portion, and a circular plate portion between the passage and the passage is bonded to the upper plate portion. 14. The method of claim 13, By adjusting the rotational speed of the second chamber and the length of the circular plate, it is possible to collect blood components according to the blood component layer formed inside by the centrifugal force of the second chamber, and the physiological buffer solution other than the regenerative cells And regulating an amount remaining in the chamber. The apparatus according to claim 1, The first inner inlet portion and the second inner inlet portion, A circular plate integrally formed at a lower end of the first inner inlet portion and the second inner inlet portion and having a slope equal to the slope of the upper plate portion and extending in a lance shape to form the passage together with the upper plate portion; And And a hollow flat disc-shaped selective discharge portion surrounding the outer side of the second inner inlet portion of the lower plate portion and selectively connecting at least one of the passages to the second outlet pipe, Wherein at least one passage exists between the upper plate portion and the lower plate portion, a circular plate portion between the passage and the passage is bonded to the upper plate portion, When separating the circular plate in the radial direction, the separated half portions have the same weight, the lengths of the passages formed in one half portion are different from each other, and the mutually opposing passages Have the same length, On the upper surface of the selective discharge portion, And a top discharge hole connected to the second discharge pipe at both sides of the through hole is formed at the center, A lateral side discharge hole is formed on a side surface thereof and connected to the selected passage, a lower end of the side surface is coupled to a coupling groove formed on an upper surface of the circular plate, Wherein the selective discharging portion is tightly coupled to the coupling groove and then rotated to select the passage. The method according to claim 1, Characterized in that a filter screen capable of holding undigested tissue or a collagen mass among the cells suspended in the liquid injected through the injection tube is further provided inside the lower body part under the injection tube Extraction system. The apparatus according to claim 1, The first inner inlet portion and the second inner inlet portion, A circular plate integrally formed at a lower end of the first inner inlet portion and the second inner inlet portion and having a slope equal to the slope of the upper plate portion, A circular plate portion having a plurality of cylindrical vanes; And A hollow flat disc-shaped selective discharge unit which surrounds the outside of the second inner inlet part of the lower plate part and selects at least one or more cylindrical vanes from the cylindrical vanes and connects the passage inside the selected cylindrical vane to the second outlet tube Respectively, The upper surfaces of the cylindrical vanes are bonded to the upper plate portion and the lengths thereof are different from each other so that the blood component layer formed inside the chamber can be collected by the centrifugal force of the second chamber, On the upper surface of the selective discharge portion, And a top discharge hole connected to the second discharge pipe at both sides of the through hole is formed at the center, A lateral side discharge hole is formed on a side surface thereof and connected to the selected passage, a lower end of the side surface is coupled to a coupling groove formed on an upper surface of the circular plate, Wherein the selective discharging portion is tightly coupled to the coupling groove and then rotated to select the cylindrical vane. The apparatus according to claim 1, The first inner inlet portion and the second inner inlet portion, A circular plate integrally formed at a lower end of the first inner inlet portion and the second inner inlet portion and having a slope equal to the slope of the upper plate portion; And a circular plate part having a plurality of cylindrical vanes, the passage being formed on the inner side and being weight-mounted on the other side end, Wherein the cylindrical wings are made of an elastic material and each weight weight mounted on each of the cylindrical wings has different weights or weights for balancing the cylindrical wings and has a centrifugal force Wherein the cylindrical vanes are opened in the direction of the inner wall surface of the second chamber, and component blood can be collected according to the blood component layer. 19. The method of claim 18, Wherein the circular plate portion has two symmetrical cylindrical blades, and the weights mounted on the cylindrical blades have the same weight. delete delete delete delete delete

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