KR100394779B1 - Organic fertilizer containing nitrogen fixing microorganisms and its manufacturing method - Google Patents
Organic fertilizer containing nitrogen fixing microorganisms and its manufacturing method Download PDFInfo
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- KR100394779B1 KR100394779B1 KR10-2000-0034953A KR20000034953A KR100394779B1 KR 100394779 B1 KR100394779 B1 KR 100394779B1 KR 20000034953 A KR20000034953 A KR 20000034953A KR 100394779 B1 KR100394779 B1 KR 100394779B1
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- nitrogen
- organic fertilizer
- microorganisms
- fixed
- polymer gel
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 75
- 244000005700 microbiome Species 0.000 title claims abstract description 68
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims description 21
- 229910052757 nitrogen Inorganic materials 0.000 title claims description 10
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 230000000813 microbial effect Effects 0.000 claims abstract description 15
- 241000589151 Azotobacter Species 0.000 claims abstract description 13
- 229920003169 water-soluble polymer Polymers 0.000 claims abstract description 8
- 239000003337 fertilizer Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 229920000642 polymer Polymers 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 11
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- 239000001963 growth medium Substances 0.000 claims description 5
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- 239000002374 bone meal Substances 0.000 description 4
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
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- 241000251468 Actinopterygii Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
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- 239000011780 sodium chloride Substances 0.000 description 2
- -1 solar energy Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
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- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001668579 Pasteuria Species 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 244000008020 Protea mellifera Species 0.000 description 1
- 235000006583 Protea mellifera Nutrition 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 235000013240 Rhus ovata Nutrition 0.000 description 1
- 235000019774 Rice Bran oil Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000012867 bioactive agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000012206 bottled water Nutrition 0.000 description 1
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- 235000019438 castor oil Nutrition 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
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- 229960002433 cysteine Drugs 0.000 description 1
- 239000006617 diazotrophic medium Substances 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
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- 238000012851 eutrophication Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000006477 glucose yeast extract medium Substances 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-L malate(2-) Chemical compound [O-]C(=O)C(O)CC([O-])=O BJEPYKJPYRNKOW-UHFFFAOYSA-L 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 150000002829 nitrogen Chemical class 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 239000007671 pyg medium Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
본 발명은 질소고정 미생물을 함유하는 유기비료 및 그 제조방법에 관한 것으로 본 발명의 유기비료는 저분자량 수용성 폴리머 젤용액, 아조토박터 빈란디, 클로스트리디움 파스퇴리아눔, 미생물 영양분을 포함하는 자연 친화적인 미생물제재이다. 본 발명은 식물의 생육을 촉진시킬 뿐만 아니라 자연의 미생물을 이용하기 때문에 환경 친화적이며 경제적인 질소고정 미생물을 함유한 유기비료와 그 제조방법을 제공한다.The present invention relates to an organic fertilizer containing nitrogen-fixed microorganisms and a method for producing the organic fertilizer of the present invention comprises a low molecular weight water-soluble polymer gel solution, azotobacter vilandi, Clostridium pasteurinum, microbial nutrients Naturally friendly microbial product. The present invention provides an organic fertilizer containing nitrogen-fixed microorganisms, which are environmentally friendly and economical, as well as promoting the growth of plants and using natural microorganisms, and a method of manufacturing the same.
Description
본 발명은 질소고정 미생물을 함유하는 유기비료 및 그 제조방법에 관한 것으로서, 더욱 상세하게는 토양에서 분리한 질소고정 미생물을 수용성 폴리머 젤용액에 혼합하여 토양에 제공함으로써 토질을 개선시키고 식물의 생육을 촉진시키는 유기비료 및 그 제조방법에 관한 것이다.The present invention relates to an organic fertilizer containing nitrogen-fixed microorganisms and a method for manufacturing the same, and more particularly, by mixing nitrogen-fixed microorganisms separated from the soil in a water-soluble polymer gel solution to the soil to improve the soil quality and plant growth It relates to an organic fertilizer to promote and a method for producing the same.
비료는 식물이 잘 자랄 수 있도록 인위적으로 배지에 주는 물질로서 세계 여러 나라에서 농업이 정착함에 따라 발전하였고, 인구증가에 따른 식량 소비량 증가로 인하여 연작이나 다모작을 실시하면서 지력(地力)의 회복을 위해 개발되어 왔다.Fertilizers are artificially added to the medium to help plants grow well, and have been developed as agriculture has settled in many countries around the world. Has been developed.
화학비료는 1839년에 독일의 화학자 J. 리비히가 골분에 황산을 작용시켜 수용성 인산을 만든 것을 기원으로 하여 발전하였으며 20세기초 공기 중의 질소로부터 암모니아를 합성하는 방법이 공업적으로 성공한 이후 생산량이 급격히 증가하여 현재는 일반화되어 있다. 또한 화학공업의 발전에 힘입어 대량생산이 가능하게 됨으로써 품질이 균일하고 값이 싸며 퇴비 등에 비해 위생적이고 사용하기 용이할 뿐만 아니라 질소, 인, 칼슘 등의 유효성분 함유량이 높은 화학비료의 사용량이 비약적으로 증대하였다. 하지만 화학비료는 이미 그 폐해가 알려진 바와 같이 장기간에 걸쳐 연속적으로 사용하면 토양이 산성화되거나 토양의 유기물이 감소하고, 이로 인하여 지력이 감소하여 생산량 감소를 초래하게 되므로 기대하는 생산량을 유지하기 위해 더욱 많은 양의 화학비료를 사용하는 악순환이 되풀이된다. 또한 화학비료의 사용으로 토양의 단립조직이 감소하면 토양이 쉽게 고갈되어 빗물 등의 침식을 잘 받게 되며 화학비료로부터 유출되는 질소나 인이 원인이 되어 하천·호수 등의 부영양화(富營養化)에 의한 환경오염이 발생되고 있다.Chemical fertilizers were developed in 1839 by the German chemist J. Liebig as a result of the action of sulfuric acid on bone meal to produce water-soluble phosphoric acid.In the early 20th century, production increased dramatically after industrial success in the synthesis of ammonia from nitrogen in the air. It is now generalized. In addition, due to the development of the chemical industry, mass production is possible, so that the quality is uniform, inexpensive, hygienic and easy to use compared to composting, and the use of chemical fertilizers with high content of nitrogen, phosphorus, calcium, etc. Increased to. However, chemical fertilizers are already known for their long-term use, and their continuous use over a long period of time can lead to acidification of the soil or reduction of organic matter in the soil, which in turn leads to a reduction in yield, resulting in reduced production. The vicious cycle of using a large amount of chemical fertilizers is repeated. In addition, if the soil monolithic structure is reduced due to the use of chemical fertilizers, the soil is easily depleted and subjected to erosion such as rainwater, which is caused by nitrogen or phosphorus flowing out of the chemical fertilizers, resulting in eutrophication of rivers and lakes. Environmental pollution occurs.
이러한 단점을 극복하기 위하여 대두되고 있는 유기비료는 천연의 유기물 중에서 식물에 유효한 비료성분을 함유하고 있는 것으로 어박분말 등의 어박류, 채종유 찌꺼기, 피마자유 찌꺼기, 쌀겨유 찌꺼기 등의 기름 찌꺼기류, 생골분(生骨粉)·증제골분(蒸製骨粉) 등의 동물성 폐기물류와 성분함량이나 품질이 일정하지 않은 퇴비, 깻묵, 골분 등이 있다. 하지만 유기비료는 일반적으로 화학비료에 비해 유효성분의 함량이 낮고 토양에 사용할 경우에는 수일의 분해기간이 필요하며 유해가스발생 등의 극복해야할 문제점들이 있다. 현재 알려진 유기비료는 제지슬러지를 이용한 유기비료(특허공개 제 98-065113호), 폐기물을 이용한 유기비료(특허공고 제 10-0222637호), 입상재배용 발효생성물질 배양토(특허공개 제 99-73201호), 효소제를 이용한 비료 등이 있지만 상기의 유기비료를 준비하는데 소요되는 많은 시간과 노력에 비해 그 효과는 미흡하다.Organic fertilizers, which are emerging to overcome these disadvantages, contain fertilizers that are effective for plants among natural organic substances, such as fish powder such as fish powder, rapeseed oil residue, castor oil residue, rice bran oil residue, etc. Animal wastes such as bone meal and powdered bone meal, and compost, ink, and bone meal are not consistent in content or quality. However, organic fertilizers generally have a lower content of active ingredients than chemical fertilizers and require several days to break down when used in soil, and there are problems to overcome such as generation of harmful gases. Currently known organic fertilizers include organic fertilizers using paper sludge (Patent Publication No. 98-065113), organic fertilizers using waste (Patent Publication No. 10-0222637), and culture soils for fermentation products for granular cultivation (Patent Publication No. 99-73201). ), Fertilizers using enzymes, but the effect is insufficient compared to the time and effort required to prepare the organic fertilizer.
또한 유기비료를 사용하면 식물체가 유기비료 자체를 그대로 흡수 이용하는 것이 아니고, 먼저 분해되어 질소, 인산, 칼슘으로 전환된 후에 흡수 이용되며, 질소원은 암모니화작용(Ammonificaton), 질산화작용(Nitrificati on)을 거쳐 질산염으로 분해되어야 흡수 이용될 수 있어 단시일내 효과를 보긴 힘들다.In addition, when organic fertilizers are used, plants do not absorb organic fertilizers themselves, but are first decomposed and converted to nitrogen, phosphate, and calcium, and then used for absorption. Nitrogen sources are used for ammonificaton and nitrificati on. It is difficult to see the effect in a short time because it can be used after being decomposed into nitrate.
최근에 유기비료로서 가능성을 보이는 방법은 자연적으로 일어나는 자연정화방법이나 토양 미생물을 활용방법으로 이러한 방법은 생태계를 유지하면서도 자연친화적으로 식물생육을 촉진시킬 수 있어 유기비료의 최선책으로 여겨지고 있으나 아직 연구, 개발이 미진한 실정이다.Recently, the method that shows potential as organic fertilizer is a natural purification method or a method using soil microorganisms. Such a method is considered as the best method of organic fertilizer because it can promote plant growth in an eco-friendly manner while maintaining an ecosystem. There is little development.
따라서 본 발명은 토질 개선과 더불어 농산물의 품질 및 수확량을 증대시킬 수 있는 질소고정 미생물을 함유하는 유기비료를 제공하는 것을 목적으로 한다.Accordingly, an object of the present invention is to provide an organic fertilizer containing nitrogen-fixed microorganisms that can improve the quality and yield of agricultural products as well as improving the soil.
또한 본 발명은 질소고정 미생물이 함유된 유기비료 제조방법을 제공하는 것을 목적으로 한다.It is another object of the present invention to provide a method for producing organic fertilizer containing nitrogen-fixed microorganisms.
도 1은 본 발명의 유기비료내의 폴리머가 질소고정 미생물을 포함하여 마이셀을 형성한 형상을 나타낸 것이고,Figure 1 shows the polymer in the organic fertilizer of the present invention to form a micelle including a nitrogen-fixed microorganism,
도 2는 본 발명의 유기비료를 사용한 고추의 생육정도를 유기비료를 사용하지 않은 대조군과 비교하여 사진으로 나타낸 것이고,Figure 2 shows the growth of the pepper using the organic fertilizer of the present invention as a photograph compared to the control group without the organic fertilizer,
도 3은 본 발명의 유기비료를 사용한 토마토의 생육정도를 유기비료를 사용하지 않은 대조군과 비교하여 사진으로 나타낸 것이고,3 is a photograph showing the growth degree of the tomato using the organic fertilizer of the present invention compared to the control group not using the organic fertilizer,
도 4는 본 발명의 유기비료를 시비하였을 때 국화배양토의 pH를 7일마다 관찰한 것이고,Figure 4 shows the pH of the chrysanthemum culture when fertilizing the organic fertilizer of the present invention every 7 days,
도 5는 본 발명의 유기비료 원액의 희석정도에 따라 국화 생육에 미치는 영향을 본 것이고,Figure 5 shows the effect on the growth of chrysanthemum according to the degree of dilution of the organic fertilizer stock solution of the present invention,
도 6은 본 발명의 유기비료를 사용한 쑥갓의 생육정도를 유기비료를 사용하지 않은 대조군과 비교하여 사진으로 나타낸 것이고,6 is a photograph showing the growth degree of mugwort using the organic fertilizer of the present invention in comparison with a control group not using organic fertilizer,
도 7은 본 발명의 유기비료를 사용한 삼엽채의 생육정도를 유기비료를 사용하지 않은 대조군과 비교하여 사진으로 나타낸 것이다.Figure 7 shows the growth of the three leaf vegetable using the organic fertilizer of the present invention compared with the control group without the organic fertilizer is shown in the photograph.
상기의 목적을 달성하기 위하여 본 발명은 질소를 고정하는 미생물을 포함하는 식물 성장 촉진제를 제공한다.In order to achieve the above object, the present invention provides a plant growth accelerator comprising a microorganism for fixing nitrogen.
또한 본 발명은 폴리머 젤용액이 미생물을 포함하여 마이셀을 형성하는 것을 특징으로 하는 유기비료용 조성물을 제공한다.In another aspect, the present invention provides a composition for an organic fertilizer, characterized in that the polymer gel solution to form micelles, including microorganisms.
본 발명은 저분자량 수용성 폴리머 젤용액; 질소고정 미생물 아조토박터 빈란디와 클로스트리디움 파스퇴리아눔; 및 미생물 영양분을 포함하는 질소고정 미생물을 포함하는 유기비료를 제공한다.The present invention is a low molecular weight water-soluble polymer gel solution; Nitrogen-fixed microorganisms Azotobacter vilandi and Clostridium pasteurinum; And it provides an organic fertilizer comprising nitrogen-fixed microorganisms containing microbial nutrients.
또한 본 발명은 중성인 저분자량 수용성 폴리머 젤용액에 미생물 영양분을 첨가하는 혼합단계; 질소고정 미생물을 배양하여 수득하는 배양단계; 및 상기 배양단계에서 수득한 질소고정 미생물을 상기 혼합단계의 폴리머 젤용액내에 혼합한 후 냉각시키는 냉각단계로 이루어지는 질소고정 미생물을 포함하는 유기비료 제조방법을 제공한다.In another aspect, the present invention is a mixing step of adding microbial nutrients to a neutral low molecular weight water-soluble polymer gel solution; A culture step obtained by culturing nitrogen-fixed microorganisms; And it provides an organic fertilizer manufacturing method comprising a nitrogen-fixed microorganism consisting of a cooling step of cooling after mixing the nitrogen-fixed microorganism obtained in the culture step in the polymer gel solution of the mixing step.
본 발명은 질소고정 미생물을 함유하는 유기비료를 물에 희석하여 씨뿌리기 2주전에, 이식을 하는 식물은 씨 뿌리기 3주전에 살포하며 이미 씨를 뿌렸거나 식물이 자라난 후에도 살포 가능한 유기비료의 살포방법을 제공한다.The present invention dilutes organic fertilizer containing nitrogen-fixed microorganisms in water 2 weeks before seeding, the transplanted plant is sprayed 3 weeks before seeding and spraying the organic fertilizer that can be sprayed even after planting or plant growth To provide.
이하 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
토양 미생물은 토양에 존재하는 미생물로 다양한 대사능력을 가져 무기형태의 물질, 태양에너지, 그리고 유기물질을 에너지원으로 이용할 수 있으며, 토양 미생물은 토양에서 영양분 분해, 질소고정, 오염 유발물질의 해독기능, 유기태 오염원의 해독, 살충제의 분해작용, 산업폐기물 분해, 기타 유해세균의 오염방지 등의 역할을 하고 있어 생물활성제로서 새롭게 대두되고 있다. 이러한 토양 미생물 중 본 발명의 질소고정 미생물은 완전한 자연 유기물질로 환경에 해가 되지 않으면서 식물에 필요한 영양분을 공급한다.Soil microorganisms are microorganisms present in the soil that have various metabolic abilities and can use inorganic materials, solar energy, and organic materials as energy sources. Soil microorganisms detoxify nutrients, fix nitrogen, and pollutants in soil. It is emerging as a bioactive agent because it plays a role in detoxifying organic pollutants, decomposing pesticides, decomposing industrial waste, and preventing contamination of other harmful bacteria. Among these soil microorganisms, the nitrogen-fixed microorganism of the present invention is a completely natural organic substance and supplies nutrients necessary for plants without harming the environment.
본 발명의 유기비료는 질소고정 미생물, 저분자량의 폴리머, 미생물 영양물질로 이루어져 있으며, 질소고정 미생물은 아조토박터 빈란디(Azotobactervinelandii) 및 클로스트리디움 파스퇴리아눔(Clostridium pasteurianum)이 바람직하며 아조토박터 빈란디 JC-01 KCTC 0795BP, 클로스트리디움 파스퇴리아눔 JC-02 KCTC 0796BP가 가장 바람직하다. 상기의 아조토박터 빈란디와 클로스트리디움 파스퇴리아눔은 대기 중의 질소가스를 식물이 이용 가능한 질소로 고정하여 식물뿌리에 제공하고 식물은 변형된 질소를 흡수하여 필요한 아미노산을 만들게 된다. 질소고정 미생물이 유기비료에 0.1 중량% 내지 20 중량%로 포함되는 것이 바람직하며본 발명의 유기비료 1 ml 당 아조토박터 빈란디가 3.5 x 103cfu이상, 클로스트리디움 파스퇴리아눔이 3.2 x 104cfu 이상 포함되는 것이 바람직하다.The organic fertilizer of the present invention is composed of nitrogen-fixed microorganisms, low molecular weight polymers, microbial nutritional substances, nitrogen-fixed microorganisms are preferred Azotobacter v inelandii and Clostridium pasteurianum ( Clostridium pasteurianum ) Most preferred are Azotobacter Vinlandi JC-01 KCTC 0795BP and Clostridium Pasteurinum JC-02 KCTC 0796BP. The azotobacter vilandi and Clostridium pasteurinum fix the nitrogen gas in the atmosphere with nitrogen available to the plant and provide it to the plant root, and the plant absorbs the modified nitrogen to make the necessary amino acids. Nitrogen-fixed microorganisms are preferably included in the organic fertilizer 0.1% by weight to 20% by weight, azotobacter vilandi more than 3.5 x 10 3 cfu per 1 ml of organic fertilizer of the present invention, Clostridium pasteurinum 3.2 x 10 4 cfu or more is preferably included.
또한 본 발명의 저분자량의 폴리머는 단풍나무(maple) 시럽이 바람직하고 상기 단풍나무 시럽은 고농도로 존재할 때 마이셀을 형성한다. 따라서 도 1에서 도시한 바와 같이 본 발명의 폴리머(①)가 질소고정 미생물(②)을 포함한 마이셀을 형성하여 유기비료내의 질소고정 미생물간의 해를 방지하게 된다. 본 발명의 유기비료에 폴리머가 1 중량% 내지 15 중량%으로 포함되는 것이 바람직하다.In addition, the low molecular weight polymer of the present invention is preferably maple syrup, and the maple syrup forms micelles when present in high concentrations. Therefore, as shown in FIG. 1, the polymer (①) of the present invention forms a micelle including nitrogen-fixed microorganisms (②) to prevent damage between nitrogen-fixed microorganisms in organic fertilizers. In the organic fertilizer of the present invention, it is preferable that the polymer is included in an amount of 1 wt% to 15 wt%.
본 발명의 유기비료에 포함되는 미생물 영양분은 5 중량% 내지 30 중량%의 조청, 1 중량% 내지 30 중량%의 우유, 및 40 중량% 내지 80 중량%의 염소를 제거한 물(dechlorinated water)을 포함한다.The microbial nutrients included in the organic fertilizer of the present invention include 5% to 30% by weight of crude, 1% to 30% by weight of milk, and 40% to 80% by weight of dechlorinated water. do.
본 발명의 질소고정 미생물을 함유하는 유기비료의 제조방법은 중성인 저분자량 수용성 폴리머 젤용액에 미생물 영양분을 첨가하는 혼합단계; 질소고정 미생물을 배양하여 수득하는 배양단계; 및 상기 배양단계에서 수득한 질소고정 미생물을 상기 혼합단계의 폴리머 젤용액내에 혼합한 후 냉각시키는 냉각단계로 이루어지며 하기에 각 단계별로 상세히 설명한다.The method for producing an organic fertilizer containing nitrogen-fixed microorganism of the present invention comprises the steps of adding microbial nutrients to a neutral low molecular weight water-soluble polymer gel solution; A culture step obtained by culturing nitrogen-fixed microorganisms; And a cooling step in which the nitrogen-fixed microorganism obtained in the culture step is mixed in the polymer gel solution of the mixing step and then cooled, and described in detail for each step below.
(1) 혼합단계: 폴리머 젤용액 및 미생물 영양분을 혼합하여 혼합액을 준비한다. 이때 폴리머 젤용액은 유기비료 전체 조성물에 대하여 1 중량% 내지 15 중량%로 사용하며, 미생물 영양분은 유기비료 전체 조성물에 대하여 50 중량% 내지 98 중량%로 사용한다.(2) 배양단계 : 호기성 및 혐기성 그룹을 모두 포함하는 질소고정 미생물을 배양배지에 접종하여 통기(aeration) 후 25 내지 42 ℃에서 혐기성 조건으로 1주일간 배양하고 다시 20분간 통기한 다음 혐기성 조건으로 1주일 배양한다. 배양 후 배양된 질소고정 미생물만 수득하였다.(1) Mixing step: prepare a mixed solution by mixing the polymer gel solution and microbial nutrients. In this case, the polymer gel solution is used in an amount of 1 wt% to 15 wt% based on the total organic fertilizer composition, and the microbial nutrient is used in an amount of 50 wt% to 98 wt% based on the total organic fertilizer composition. (2) Culture step: aerobic and Nitrogen-fixed microorganisms including all anaerobic groups were inoculated into the culture medium, and then incubated for one week under anaerobic conditions at 25 to 42 ° C. after aeration, followed by aeration for 20 minutes, followed by one week under anaerobic conditions. Only cultured nitrogen-fixed microorganisms were obtained after the culture.
(3) 냉각단계: 상기에서 수득한 질소고정 미생물은 상기 혼합단계에서 준비한 혼합액에 혼합한 다음 8 ℃로 냉각시킨다. 이때 질소고정 미생물의 함량은 유기비료 전체 조성물에 대하여 0.1 내지 20 중량%이다.(3) Cooling step: The nitrogen-fixed microorganism obtained above is mixed with the mixed solution prepared in the mixing step and then cooled to 8 ° C. At this time, the content of the nitrogen-fixed microorganism is 0.1 to 20% by weight based on the total organic fertilizer composition.
상기의 질소고정 미생물의 배양단계에서 혐기적 조건과 호기적 조건이 교대로 변환되어도 질소고정 미생물의 생존에는 별 영향을 미치지 않는다. 본 발명의 클로스트리디움 파스퇴리아의 배양배지는 글루코스 이스트 추출액배지(글루코스 20 g, 이스트 추출액 10 g, CaCO320 g, 아가 17 g, 멸균수를 첨가하여 배지 1L를 제조)와 PYG-배지(트립티케이즈 펩톤 5 g, 펩톤 5 g, 이스트추출액 10 g, 비프추출액 5 g, 글루코스 5 g, K2HPO42 g, 레사주린(resazurin) 1 mg, 시스테인-HCl 0.5 g, 염용액 40 ml, 멸균수 961ml, 상기 염용액은 CaCl2x 2H2O 0.25 g, MgSO4x 7H2O 0.5 g, K2HPO41 g, KH2PO41 g, NaHCO310 g, NaCl 2 g, 및 멸균수를 가하여 1000 ml을 제조한다.)가 바람직하고, 아조토박터의 배양배지는 RBA배지(diazotrophic medium:A 용액 + B 용액 + C 용액, A 용액; KH2PO40.1 g, K2HPO40.9 g, NaCl 0.1 g, CaCl2x 2H2O 0.1 g, MgSO4x 7H2O 0.1 g, Na2MoO4x 2H2O 0.005 g, NaVO3x H2O 0.005 g, MnSO4x H2O 0.005 g, FeSO4x 7H2O 0.01 g, Trace delement sol. SL-6 3 ml, 멸균수 900 ml, B 용액; 이스트추출액 0.05 g, Na2-succinate 1 g, DL-malate 2 g, 멸균수 50 ml, C 용액; Na-pyruvate 1 g, D-mannitol 2 g, D-glucose 2 g, 멸균수 50 ml)가 바람직하다.Even if anaerobic conditions and aerobic conditions are alternately converted in the culture step of nitrogen-fixed microorganisms, it does not affect survival of nitrogen-fixed microorganisms. The culture medium of Clostridium Pasteuria of the present invention is a glucose yeast extract medium (glucose 20 g, yeast extract 10 g, CaCO 3 20 g, agar 17 g, sterile water is added to prepare a medium 1L) and PYG-medium (5 g trypticase peptone, 5 g peptone, 10 g yeast extract, 5 g beef extract, 5 g glucose, 2 g K 2 HPO 4 , 1 mg resazurin, 0.5 g cysteine-HCl, salt solution 40 ml, sterile water 961 ml, the salt solution is CaCl 2 x 2H 2 O 0.25 g, MgSO 4 x 7H 2 O 0.5 g, K 2 HPO 4 1 g, KH 2 PO 4 1 g, NaHCO 3 10 g, NaCl 2 g , And sterile water is added to prepare 1000 ml.), And the culture medium of azotobacter is RBA medium (diazotrophic medium: A solution + B solution + C solution, A solution; KH 2 PO 4 0.1 g, K 2 HPO 4 0.9 g, NaCl 0.1 g, CaCl 2 x 2H 2 O 0.1 g, MgSO 4 x 7H 2 O 0.1 g, Na 2 MoO 4 x 2H 2 O 0.005 g, NaVO 3 x H 2 O 0.005 g, MnSO 4 0.005 g x H 2 O, 0.01 g FeSO 4 x 7H 2 O, Trace delement sol.SL-6 3 ml, sterile water 900 ml, for B Solution; yeast extract 0.05 g, Na 2 -succinate 1 g, DL-malate 2 g, sterile water 50 ml, C solution; Na-pyruvate 1 g, D-mannitol 2 g, D-glucose 2 g, sterile water 50 ml Is preferred.
상기의 제조방법으로 제조한 미생물 함유 유기비료의 질소고정 미생물은 상기 폴리머와 4 내지 5 ㎛의 마이셀(micell)을 형성하여 혼합액내에서 부유상태로 있으면서 모든 활동이 중지된 상태로 존재하게 된다.Nitrogen-fixed microorganisms of the microorganism-containing organic fertilizer prepared by the above production method forms a micelle of 4 to 5 μm with the polymer and is present in a suspended state in the mixed solution while all activities are suspended.
또한 본 발명의 유기비료는 토양에 뿌려짐과 동시에 미생물의 활동을 중단시켜 토질의 불균형을 초래하는 질소비료와는 달리, 동종의 토양 미생물과 상조작용(synergism)으로 토질을 개선키고 토양 미생물의 수소 및 산소 이온을 조절하는 기능으로 인하여 토질의 pH를 자동적으로 중성화시켜 식물이 성장하기에 균형적인 환경조건을 갖추게 한다.In addition, the organic fertilizer of the present invention, unlike the nitrogen fertilizer that is sprayed on the soil and at the same time stop the activity of microorganisms cause imbalance of soil, improve the soil by synergism with the same soil microorganisms (hydrogen of the soil microorganisms) And due to the ability to control the oxygen ions to neutralize the pH of the soil automatically to have a balanced environmental conditions for plants to grow.
본 발명의 유기비료의 사용 방법은 먼저 유기비료와 물을 1:100의 비율로 혼합하여 사용하기 전에 3일 내지 5일 동안 햇빛 또는 열기속에 방치시키며, 대기 온도가 24 ℃이하인 경우 매 5 ℃마다 1일을 더 첨가하여 방치시킨다. 이는 유기비료의 최고 효과를 얻기 위해 고정된 용제가 작은 분자로 침투하거나 새롭게 배양된 유기비료내로 균등하게 팽창하도록 하기 위함이다. 본 발명의 유기비료는 물에 1 : 100비율로 희석한후 시비전에 다시 물에 10배로 희석하여 토지 평당 50 ℓ 내지 250 ℓ의 비율로 사용하며 벼농사 등의 물을 많이 대어서 유기비료가 묽어지는 작물에는 평당 50 ℓ내지 300 ℓ을 사용하는 것이 바람직하고, 재배하는 작물이 나무와 같이 8피트 이상 크거나 커질 것이라면 모든 뿌리 시스템에 유기비료를 충분히 침투시키기 위해 평당 50 ℓ내지 300 ℓ을 사용하는 것이 바람직하다.The method of using the organic fertilizer of the present invention is first mixed with organic fertilizer and water in a ratio of 1: 100, and left in sunlight or heat for 3 to 5 days before use, every 5 ℃ when the atmospheric temperature is 24 ℃ or less Leave for 1 more day. This is to allow the fixed solvent to penetrate into small molecules or to expand evenly into the newly cultured organic fertilizer in order to obtain the best effect of the organic fertilizer. The organic fertilizer of the present invention is diluted in water at a ratio of 1: 100, and then diluted 10 times with water before fertilization, and is used at a rate of 50 ℓ to 250 ℓ per land pyeong, and the organic fertilizer is diluted by applying a lot of water such as rice farming. It is preferable to use 50 L to 300 L per pyeong, and if the crops to be grown will be larger or larger than 8 feet like trees, it is preferable to use 50 L to 300 L per pyeong to sufficiently infiltrate organic fertilizer into all root systems. Do.
또한 본 발명의 유기비료를 살포하는 시기는 씨뿌리기 2주전이 바람직하고이식을 하는 식물은 씨 뿌리기 3주전에 살포하는 것이 바람직하지만 이미 씨를 뿌렸거나 식물이 자라난 후에도 살포할 수 있다. 상기에서 언급한 바와 같이 살포양과 살포시기를 결정한 후 물에 희석한 본 발명의 유기비료는 촉촉한 토양에 살포하거나 살포 후 물을 붓는 것이 바람직하다. 또한 본 발명의 유기비료는 통상의 살포기를 사용하여 흩어 살포하거나 부어 사용하며 건조한 비료나 유황, 칼륨, 카본 등과 같은 화학물질이나 짚 찌꺼기 같은 원료에 첨가하여 사용할 수 있다.In addition, the timing of spraying the organic fertilizer of the present invention is preferably two weeks before seeding, and the plant to be transplanted is preferably sprayed three weeks before seeding, but can also be sprayed after seeding or plant growth. As mentioned above, the organic fertilizer of the present invention diluted in water after determining the amount of spraying and the time of spraying is preferably sprayed on moist soil or poured water after spraying. In addition, the organic fertilizer of the present invention can be used to spread or pour by using a conventional spreader, and can be used by adding to raw materials such as dry fertilizers, chemicals such as sulfur, potassium, carbon or straw.
본 발명에서 질소고정 미생물을 함유한 유기비료의 효능을 알아보고자 쑥갓, 삼엽채, 고추, 토마토에 대한 생육실험을 수행하였다. 그 결과 쑥갓은 초장, 잎크기, 지상부층이 증가되고 특히 뿌리의 생장이 향상되었으며 삼엽채 또한 초장, 엽장, 엽폭, 근장, 생체중이 증가되었다. 고추는 지상부와 지하부의 생육이 촉진되었고 엽색이 암록색으로 짙어졌으며, 토마토는 외관상 별 효과가 없었으나 개화가 촉진되어 열매가 빨리 형성되었다. 또한 본 발명에서 질소고정 미생물을 함유한 유기비료를 살포한 후 토양의 pH를 측정한 결과 살포한 토양의 pH가 중성화되어 산성토양일 때 발생하는 병해충이 방지됨을 알 수 있었다.In order to determine the efficacy of the organic fertilizer containing nitrogen-fixed microorganisms in the present invention, growth experiments were performed on cucurbita, trifolia, pepper and tomato. As a result, wormwood was increased in height, leaf size and ground layer, especially root growth was improved, and trifoliate was also increased in height, leaf length, leaf width, root length, and live weight. The red pepper was accelerated in the growth of the ground and the underground, and the leaf color was dark green. The tomato had no effect in appearance, but the flowering was accelerated, and fruit was formed quickly. In addition, in the present invention, after spraying the organic fertilizer containing nitrogen-fixed microorganisms, the pH of the soil was measured, it can be seen that the pests generated when the soil pH is neutralized and acidic soil is prevented.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시한다. 그러나 하기의 실시예는 본 발명을 보다 쉽게 이해하기 위하여 제공되는 것일 뿐 본 발명이 하기의 실시예에 한정되는 것은 아니다.Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.
[실시예]EXAMPLE
먼저 상온에서 저분자량 폴리머로 단풍나무 시럽(Turkey Hill Sugarbush Ltd., 10 waterloo st. WATERLOO, QUEBEC J0E2N0, CANADA) 3 중량%에 대하여 미생물 영양분으로 17 중량%의 조청, 9 중량%의 우유, 69 중량%의 염소가 제거된 물(dechlorinated water)을 첨가하여 혼합액을 준비하였다. 그리고 질소고정 미생물 아조토박터 빈란디와 클로스트리디움 파스퇴리아눔을 배양하기 위하여 물을 끓인 후 식히고 배양배지와 발효 이스트(Saccharomyces cerevisiae)을 첨가하여 25 ℃에서 일주일간 발효시킨다. 발효가 끝나고 발효 이스트를 여과하고 남은 여과액에 아조토박터 빈란디와 클로스트리디움 파스테리아눔을 접종하여 통기(aeration) 후 일 주일동안 혐기성 조건으로 배양한다. 상기 배양액에 염소와 물을 가한 다음 밀폐된 에어시스템에 20분 동안 통기시키고 다시 혐기성 조건에서 1주일간 배양하여 아조토박터 빈란디와 클로스트리디움 파스퇴리아눔을 원심분리로 수득하였다. 상기에서 수득한 질소고정 미생물 아조토박터 빈란디아 1 중량%와 클로스트리디움 파스퇴리아눔 1 중량%를 상기 단풍나무시럽과 미생물 영양분 혼합액에 섞은 후 8 ℃로 냉각하여 질소고정 미생물을 함유한 유기비료를 제조하였다.First, 17% by weight of crude, 9% by weight of milk, 69% by weight of microbial nutrients to 3% by weight of maple syrup (Turkey Hill Sugarbush Ltd., 10 waterloo st. WATERLOO, QUEBEC J0E2N0, CANADA) as a low molecular weight polymer at room temperature The mixed solution was prepared by adding dechlorinated water of%. In order to cultivate nitrogen-fixed microorganisms Azotobacter vilandi and Clostridium pasteurinum, water is boiled and cooled, and culture medium and fermentation yeast ( Saccharomyces cerevisiae ) are added and fermented at 25 ° C. for one week. After the fermentation is finished, the fermentation yeast is filtered, and the remaining filtrate is inoculated with azotobacter binrandidi and Clostridium pasteerianum and cultured under anaerobic conditions for one week after aeration. Chlorine and water were added to the culture solution, followed by aeration for 20 minutes in a closed air system, followed by incubation for one week under anaerobic conditions to obtain azotobacter vilandidi and Clostridium pasteurinum by centrifugation. 1% by weight of the nitrogen-fixed microorganism Azotobacter Vinlandia and 1% by weight of Clostridium pasteurinum were mixed with the maple syrup and microbial nutrient mixture, and cooled to 8 ° C. to contain organic nitrogen-fixed microorganisms. Fertilizer was prepared.
[실험예 1]Experimental Example 1
상기 실시예 1에서 제조한 유기비료를 토마토, 고추를 대상으로 실험하였다. 유기비료 원액을 생수에 1:100으로 희석하여 상온에서 5일간 방치한 후 다시 물에 10배로 희석하여 토지 1평에 150 ℓ를 살포하고 대조군으로 살균한 희석 유기비료를 동일비율로 살포하였다. 살포한 위에 살짝 흙을 덮은 후 종자를 통상적 방법에 의하여 줄뿌림하였다. 관수는 주 1-3회 정도 토양의 상태에 따라 통상적으로 실시하였고, 제초와 솎음을 2회 실시하였다. 살포하고 2주지나 파종 후 2개월간 생육시킨 식물을 수확하여 식물의 초장, 엽장, 엽폭, 줄기직경, 뿌리길이 및 식물의 지상부와 지하부의 비율 등을 측정하였다. 건물율은 80 ℃에서 3일간 건조 후 103℃에서 1일 건조하여 조사하였다.The organic fertilizer prepared in Example 1 was tested for tomatoes and peppers. The organic fertilizer stock solution was diluted 1: 100 in bottled water, left at room temperature for 5 days, and then diluted 10 times with water. After the soil was slightly covered with the spray, the seeds were lined by conventional methods. Watering was usually performed 1-3 times a week depending on soil conditions, and weeding and wetting were performed twice. Plants grown 2 weeks after sowing and 2 months after sowing were harvested to measure plant height, leaf length, leaf width, stem diameter, root length, and the ratio of the ground and underground parts of the plant. The drying rate was dried at 80 ° C. for 3 days and then dried at 103 ° C. for 1 day.
(1) 고추 및 토마토의 실험(1) pepper and tomato experiment
상기 실험을 수행하여 하기 표 1과 같은 결과를 볼 수 있었으며 고추에서는 지상부의 생육이나 지하부의 생육이 촉진되었고 특히 지하부의 뿌리생육이 증가되었다. 토마토에서는 큰 차이를 볼 수 없었으나 개화가 촉진되었다.The experiment was performed to see the results as shown in Table 1 below, and the growth of the ground portion or the growth of the ground portion in red pepper was promoted, especially the root growth of the ground portion was increased. In tomato, there was no big difference, but flowering was accelerated.
도 2는 대조군과 실시예를 처리한 고추를 사진으로 나타낸 것으로 (가)는 고추의 지상부 생육정도를 (나)는 지하부의 생육정도를 나타낸 것이다. 도 2의 실시예 1을 사용한 고추에서 고추의 측아 발달과 뿌리 증가를 볼 수 있으며 엽색이 짙어지는 현상을 볼 수 있었다. 도 3은 대조군과 실시예 1을 처리한 토마토를 사진으로 나타낸 것으로 (가)는 토마토의 지상부 생육정도를 (나)는 지하부의 생육정도를 나타낸 것이다.Figure 2 shows a picture of the pepper treated with the control group and the example (A) shows the growth of the ground portion of the pepper (B) shows the growth of the underground. In the pepper using Example 1 of Figure 2 can see the side development and root increase of the pepper and the phenomenon of darkening of the leaf. Figure 3 is a photograph of the tomato treated with the control group and Example 1 (A) shows the growth of the ground portion of the tomato (B) shows the growth of the underground portion.
[실시예 2]Example 2
상기 실시예 1과 동일한 방법으로, 본 발명의 아조토박터 빈란디JC-01 KCTC 0795BP와 클로스트리디움 파스퇴리아눔 JC-02 KCTC 0796BP를 포함한 유기비료를 제조하였다.In the same manner as in Example 1, an organic fertilizer including azotobacter binrandidi JC-01 KCTC 0795BP and Clostridium pasteurinum JC-02 KCTC 0796BP of the present invention was prepared.
[실험예 2]Experimental Example 2
상기 실시예 2의 유기비료를 원액 1/100, 2/100, 2/1000, 1/1000로 물에 희석하여 국화 배양토에 시비 후 시간에 따른 토양의 pH 변화와 국화의 생장 정도를 관찰하였다. 대조군은 실시예 2의 유기비료를 시비하지 않고 물만 공급하였다.The organic fertilizer of Example 2 was diluted with water in 1/100, 2/100, 2/1000, and 1/1000 of the stock solution, and the change in soil pH and growth of chrysanthemum were observed with time after fertilization on chrysanthemum culture soil. The control group was fed only water without fertilizing the organic fertilizer of Example 2.
도 4는 본 발명의 유기비료를 시비하였을 때 국화배양토의 pH를 7일마다 관찰한 것으로, 유기비료 시비 후 한 달이 지났을때 배양토는 pH가 6.5-6.6을 보이는 반면에 대조군은 5.7로 나타났다.Figure 4 shows the pH of the chrysanthemum culture soil every 7 days when fertilizing the organic fertilizer of the present invention, when one month after fertilizing the organic fertilizer showed a pH of 6.5-6.6, while the control was 5.7.
도 5는 본 발명의 유기비료 원액의 희석정도에 따라 국화 생육에 미치는 영향을 본 것으로, 유기비료를 1000배로 물에 희석하여 1일 1회 시비하였을 때 초장, 엽장, 근장 , 수분함량, 엽록소함량이 가장 좋았고 시비 횟수보다는 유기비료의 희석정도가 식물생육에 변수로 작용함을 알 수 있었다.Figure 5 shows the effect on the growth of chrysanthemum according to the degree of dilution of the organic fertilizer stock of the present invention, when the organic fertilizer was diluted in water 1000 times and fertilized once a day, herbaceous, leafy, root, moisture content, chlorophyll content This was the best and the dilution of organic fertilizer was more important than the number of fertilizers.
[실험예 3]Experimental Example 3
상기 실시예 2에서 제조한 유기비료를 쑥갓, 삼엽체를 대상으로 실험하였다. 실험방법은 실험예 1과 동일하게 수행하였다.The organic fertilizer prepared in Example 2 was tested on wormwood, trilobite. Experimental method was carried out in the same manner as in Experiment 1.
(1)쑥갓 실험(1) wormwood experiment
상기 실험예 1과 동일하게 실험을 수행하여 하기 표 2의 결과를 나타내었다.The experiment was performed in the same manner as in Experimental Example 1 to show the results of Table 2 below.
상기의 표에 나타난바와 같이 실시예의 쑥갓의 생육이 대조군에 비해 향상되었으며 특히 지상부의 식물 개체당 생체중은 대조군이 8.58 g이고 실시예가 9.69 g로 약 1 g정도가 증가되었다. 이는 실제로 약 13 %의 증가를 뜻한다. 지하부는 실시예의 쑥갓이 뿌리 발달이 좋아 생체중과 건물율이 좋았다. 또한 쑥갓의 생육을 비교하기 위하여 도 6에서 대조군과 실시예를 사진으로 나타내었다. 도 6에서 나타나듯이 실시예 2는 대조군에 비해 키는 비슷하나 줄기가 굵고 측아의 발생이 많은 것을 볼 수 있었으며 뿌리가 잘 발달되었음을 알 수 있었다.As shown in the above table, the growth of mugwort in the example was improved compared to the control group, and in particular, the live weight per plant population in the above ground was 8.58 g in the control group and the example was increased to about 1g to 9.69 g. This actually means an increase of about 13%. In the basement, the wormwood root of the Example had good root development and had a good living weight and dry rate. In addition, in order to compare the growth of mugwort, the control and the example in Figure 6 is shown in the photograph. As shown in Figure 6, Example 2 was similar in height to the control group, but the stem was thick and the occurrence of side buds were seen a lot, it can be seen that the root is well developed.
(2) 삼엽채 실험(2) Trilobite Experiment
상기 쑥갓 실험과 동일하게 실험을 수행한 결과, 하기 표 3과 같이 초장, 엽장, 엽폭, 근장, 생체중, 건물율이 대조군에 비해 실시예의 삼엽채가 생육이 좋았으며 뿌리생육도 증가하였다.As a result of the experiment in the same way as the mugwort experiment, as shown in Table 3, the plant height, leaf length, leaf width, root length, live weight, dry rate of the three leaf vegetables of the Example was better than the control group, root growth was also increased.
또한 도 7은 대조군과 실시예 2를 처리한 삼엽채를 사진으로 나타낸 것으로 실시예의 삼엽채의 잎이 크고 탐스러워 상품성이 있었다.In addition, Figure 7 shows the control and the three-leaf vegetable treated with Example 2 as a photograph of the leaf of the three leaf vegetables of the Example was large and greedy commercially available.
상기에 언급한 바와 같이 본 발명의 질소고정 미생물을 함유하는 유기비료는 식물 근권의 생육촉진과 작물에 따라 지상부의 생육증진을 도우며 환경오염의 문제가 없는 자연 친화적인 미생물제제이다. 또한 본 발명의 유기비료는 질소고정 미생물에 의하여 토질을 개선시키며 생물학적 균형을 유지시킨다.As mentioned above, the organic fertilizer containing the nitrogen-fixed microorganism of the present invention is a natural-friendly microbial agent that helps the growth of the ground part according to the growth promotion and crop growth of the plant root zone and does not have the problem of environmental pollution. In addition, the organic fertilizer of the present invention improves the soil by nitrogen-fixed microorganisms and maintains the biological balance.
Claims (16)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102082251B1 (en) * | 2019-12-23 | 2020-02-28 | 김근태 | Slow released liquid fertilizer and Method for Forming the Same |
| KR102516431B1 (en) | 2022-04-14 | 2023-04-03 | 케이지케미칼 주식회사 | Microbial Fertilizer for Carbon Dioxide Reduction and Preparation Method of the Same |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR800001441B1 (en) * | 1979-03-07 | 1980-12-10 | 이철호 | Fertilizer composition |
| KR950014035A (en) * | 1993-11-12 | 1995-06-15 | 강형모 | Nitrogen-fixed microbial fertilizer and its manufacturing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR800001441B1 (en) * | 1979-03-07 | 1980-12-10 | 이철호 | Fertilizer composition |
| KR950014035A (en) * | 1993-11-12 | 1995-06-15 | 강형모 | Nitrogen-fixed microbial fertilizer and its manufacturing method |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102082251B1 (en) * | 2019-12-23 | 2020-02-28 | 김근태 | Slow released liquid fertilizer and Method for Forming the Same |
| KR102516431B1 (en) | 2022-04-14 | 2023-04-03 | 케이지케미칼 주식회사 | Microbial Fertilizer for Carbon Dioxide Reduction and Preparation Method of the Same |
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