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CN108129238A - A kind of multi-functional enzyme liquid and preparation method thereof - Google Patents

A kind of multi-functional enzyme liquid and preparation method thereof Download PDF

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Publication number
CN108129238A
CN108129238A CN201810069874.0A CN201810069874A CN108129238A CN 108129238 A CN108129238 A CN 108129238A CN 201810069874 A CN201810069874 A CN 201810069874A CN 108129238 A CN108129238 A CN 108129238A
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CN
China
Prior art keywords
enzyme liquid
bacillus
functional enzyme
soil
culture solution
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Pending
Application number
CN201810069874.0A
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Chinese (zh)
Inventor
杨兴平
白二镐
李佼怀
刘林学
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenmu Da Worth Bioengineering Co Ltd
Beijing Multi Matter Technology Co Ltd
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Shenmu Da Worth Bioengineering Co Ltd
Beijing Multi Matter Technology Co Ltd
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Application filed by Shenmu Da Worth Bioengineering Co Ltd, Beijing Multi Matter Technology Co Ltd filed Critical Shenmu Da Worth Bioengineering Co Ltd
Priority to CN201810069874.0A priority Critical patent/CN108129238A/en
Publication of CN108129238A publication Critical patent/CN108129238A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • Plant Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to technical field of microbial fermentation, disclose a kind of multi-functional enzyme liquid and preparation method thereof, including:Using kuh-seng, Phytolacca acinosa or rice chaff medicinal herbs zymotic fluid is obtained according to mass ratio with sugar;Medicinal herbs zymotic fluid and culture solution are obtained into medicinal herbs zymotic fluid culture solution according to mass ratio culture;Bacillus and culture solution are obtained into bacillus culture solution according to mass ratio culture;Zymotic fluid culture solution, bacillus culture solution, trace element salt are added in into mating groove by ratio of weight and the number of copies, are concentrated to give concentrate after mixing;Concentrate is subjected to homogeneous, fermentation, obtains multi-functional enzyme liquid.The enzyme liquid can accelerate the corruption of plant stalk in soil by microbial activities, and then improve the soil organism, nutrient content, improve soil physical and chemical property and soil microflora, improve soil activation, plant nutrient, accelerate the decomposition of soil nutrient, increase microbe metabolite such as micro-element, amino acid, auximone etc., fertility is improved so as to activate soil.

Description

A kind of multi-functional enzyme liquid and preparation method thereof
Technical field
The invention belongs to technical field of microbial fermentation, and in particular to a kind of multi-functional enzyme liquid and preparation method thereof.
Background technology
Agricultural is one of China's pillar industry, and agricultural refers to the growth-development law using animals and plants, passes through artificial culture To obtain the industry of product.Agricultural belongs to the primary industry, and the science for studying agricultural is agronomy.The subject of labour of agricultural is that have life Animals and plants, the product of acquisition be animals and plants in itself.Agricultural is to provide the basic industry of the support development of the national economy and development.Agriculture Industry plantation is plant culture, including various crops, forest, fruit tree, flowers and plants, medicinal and ornamental etc. cultivations of plants, there is grain Crop, industrial crops, vegetable crop, green manure crop, forage crop, herbage etc..As science and technology continues to develop, agricultural planting technique Also it is rapidly developed.Fertilizer is one of more crucial factor in agricultural planting, and the quality of fertilizer is also largely determined Determine the success or failure of agricultural planting, with agriculture large-scale plantation, traditional farm manure has not caught up with the demand of agricultural planting.For It increases production and improves productivity, at present more the most commonly used is chemical fertilizer abbreviation chemical fertilizer in agricultural planting, chemical fertilizer refers to chemistry and (or) physics The fertilizer of nutrient needed made of method containing one or more of crop growths.Although chemical fertilizer is to a certain extent, Fertilising is facilitated, improves agricultural planting yield and output value.But largely using chemical fertilizer so that heavy metal-polluted soil is excessive, destroys Land resource causes the pollution of environment.Also, the crops of plantation are also excessive containing Partial Elements, affect the body of people Agricultural planting purpose is not achieved in health.
Invention content
Based on this, in view of the above-mentioned problems, the present invention proposes a kind of multi-functional enzyme liquid and preparation method thereof, environmental protection can be used as Ferment fertilizer realizes no pesticide cultivation, by the technique manufacture of microbial fermentation, is conducive to the absorption of crop, and fertilization effect is good, and Soil can be improved, soil fertility is made to improve.
In one embodiment, the present invention proposes a kind of preparation method of multi-functional enzyme liquid, including:
Kuh-seng, Phytolacca acinosa or rice chaff are added to according to the ratio of mass ratio 1: 0.7-1 in container, and with again by S101 with sugar Object compacting is extracted, and obtains medicinal herbs extract liquor;The medicinal herbs extract liquor and water are mixed with the ratio of mass ratio 1: 1.5-2, After fermentation, medicinal herbs zymotic fluid is obtained;
The obtained medicinal herbs zymotic fluids of S101 and culture solution are added in the first hair by S102 according to the ratio of mass ratio 1: 70-110 It in fermentation tank, cultivates 5-9 days, the sieve for crossing 65-70 mesh obtains medicinal herbs zymotic fluid culture solution;
S103 adds in bacillus and culture solution in the second fermentation tank according to the ratio of mass ratio 1: 60-100, culture 5-9 days, the sieve for crossing 60-65 mesh obtained bacillus culture solution;
S104, the gemma bar for by ratio of weight and the number of copies obtaining medicinal herbs zymotic fluid culture solution that 1 part of S102 is obtained, 1 part of S103 Bacteria culture fluid, 0.00166-0.0144 parts of trace element salts add in mating groove, and concentrated machine concentration, is concentrated after mixing Liquid;
S105, the concentrate that S104 is obtained is in 1.5Kg/cm2-3Kg/cm2Under the conditions of carry out homogeneous, fermentation, and will be equal After liquid after matter, fermentation crosses the sieve of 100-120 mesh, multi-functional enzyme liquid is obtained.
Optionally, the bacillus includes:Bacillus subtilis, bacillus megaterium, bacillus licheniformis or lactic acid Bacillus.
Optionally, extraction temperature is 20 DEG C -60 DEG C in step S101, the fermentation temperature of the medicinal herbs extract liquor for 20 DEG C - 40℃。
Optionally, the cultivation temperature in first fermentation tank in the step S102 is 36 DEG C -38 DEG C, oxygen is passed through Measure is 0.35-0.55 cubes m/h.
Optionally, the cultivation temperature in second fermentation tank in the step S103 is 30 DEG C -38 DEG C, oxygen is passed through Measure is 0.4-0.58 cubes m/h.
Optionally, the trace element salt in the step S104 is by ratio of weight and the number of copies by 0.0008-0.0014 parts of boron Hydrochlorate and 0.00003-0.00004 parts of molybdate compositions.
Optionally, the concentration condition in the step S104 is pressure 600mmHg-750mmHg, -75 DEG C of temperature 60 C.
In another embodiment, the invention also provides a kind of multi-functional enzyme liquids, and the multi-functional enzyme liquid is by described The preparation method of above-mentioned multi-functional enzyme liquid is made.
The beneficial effects of the invention are as follows:
1st, enzyme liquid provided by the invention accelerates the corruption of plant stalk etc. in soil by microbial activities, and then improves The soil organism.
2nd, enzyme liquid provided by the invention is used for agricultural planting, can effectively reduce destruction of the heavy metal to soil, preferably Land resource is protected, avoids Environment pollution.And it containing multiple nutritional components, preferably meets needed for crop growth Element improves the output value yield of agricultural planting, it is ensured that the economic benefit of agricultural planting.
3rd, enzyme liquid provided by the invention is used for agricultural planting, and pol, weight and the size of raise crop greatly carry Height, and reduce agricultural chemicals expense, fertilizer cost and labour cost, reduces cost, and the salt in soil prevents continuous cropping obstacle without accumulation, point Remains of pesticide is solved, so non-environmental-pollution, apparent to the improved effect of soil, particularly various growth is hindered and disease has Preferably prevent and prevent from acting on.
4th, enzyme liquid is prepared using method provided by the invention, processing method is simple, is easy to grasp, and production process is easy to steady Fixed control, has preferably ensured the quality of agricultural planting enzyme liquid fertilizer.
Description of the drawings
Attached drawing described herein is used to provide further understanding of the present invention, and forms the part of the application, not Form limitation of the invention.In the accompanying drawings:
Fig. 1 is the preparation method flow chart of the multi-functional enzyme liquid of one embodiment of the invention.
In the accompanying drawings, identical or corresponding label represents identical or corresponding part.
Specific embodiment
The principle and spirit of the invention are described below with reference to several illustrative embodiments.It should be appreciated that provide this A little embodiments are not with any just for the sake of better understood when those skilled in the art and then realize the present invention Mode limits the scope of the invention.On the contrary, these embodiments are provided so that the disclosure is more thorough and complete, and energy It is enough that the scope of the present disclosure is completely communicated to those skilled in the art.
Coordinate schema and presently preferred embodiments of the present invention below, the present invention is further explained to reach predetermined goal of the invention institute The technological means taken.
Fig. 1 is the preparation method flow chart of the multi-functional enzyme liquid of one embodiment of the invention.As shown in Figure 1, this method packet It includes:
Kuh-seng, Phytolacca acinosa or rice chaff are added to according to the ratio of mass ratio 1: 0.7-1 in container, and with again by S101 with sugar Object compacting is extracted, and obtains medicinal herbs extract liquor;The medicinal herbs extract liquor and water are mixed with the ratio of mass ratio 1: 1.5-2, After fermentation, medicinal herbs zymotic fluid is obtained;
The obtained medicinal herbs zymotic fluids of S101 and culture solution are added in the first hair by S102 according to the ratio of mass ratio 1: 70-110 It in fermentation tank, cultivates 5-9 days, the sieve for crossing 65-70 mesh obtains medicinal herbs zymotic fluid culture solution;
S103 adds in bacillus and culture solution in the second fermentation tank according to the ratio of mass ratio 1: 60-100, culture 5-9 days, the sieve for crossing 60-65 mesh obtained bacillus culture solution;
S104, the gemma bar for by ratio of weight and the number of copies obtaining medicinal herbs zymotic fluid culture solution that 1 part of S102 is obtained, 1 part of S103 Bacteria culture fluid, 0.00166-0.0144 parts of trace element salts add in mating groove, and concentrated machine concentration, is concentrated after mixing Liquid;
S105, the concentrate that S104 is obtained is in 1.5Kg/cm2-3Kg/cm2Under the conditions of carry out homogeneous, fermentation, and will be equal After liquid after matter, fermentation crosses the sieve of 100-120 mesh, multi-functional enzyme liquid is obtained.
From the point of view of Fig. 1, in one embodiment, the bacillus includes:Bacillus subtilis, huge gemma Bacillus, bacillus licheniformis or Bacillus acidi lactici.
In one embodiment, extraction temperature is 20 DEG C -60 DEG C in step S101, and the fermentation temperature of the medicinal herbs extract liquor is 20℃-40℃。
In one embodiment, the cultivation temperature in first fermentation tank in the step S102 is 36 DEG C -38 DEG C, oxygen Gas intake is 0.35-0.55 cubes m/h.
In one embodiment, the cultivation temperature in second fermentation tank in the step S103 is 30 DEG C -38 DEG C, oxygen Gas intake is 0.4-0.58 cubes m/h.
In one embodiment, the trace element salt in the step S104 is by ratio of weight and the number of copies by 0.0008- 0.0014 part of borate and 0.00003-0.00004 parts of molybdate compositions.
In one embodiment, the concentration condition in the step S104 for pressure 600mmHg-750mmHg, temperature 60 C- 75℃。
In another embodiment, the invention also provides a kind of multi-functional enzyme liquids, and the multi-functional enzyme liquid is by described The preparation method of above-mentioned multi-functional enzyme liquid is made.
Wherein, the preponderant functional bacteria of ferment bacterium mainly includes saccharomycete, bacillus subtilis, bacillus licheniformis, huge Bacillus, Bacillus acidi lactici aspergillus oryzae etc..
Specifically, saccharomycete:With good Fermentation Function, thalline micro-element, protein content hypermetabolism product contain Nucleic acid, glutathione, coacetylase, ATP, biotin, thiamine.
Bacillus subtilis:Main function is to generate poly- wheat bran amine acid, is soil protection film, effectively prevent supporting Divide, the loss of moisture;Generate high activity catabolic enzyme, it may be difficult to which the macromolecular substances utilized resolve into available small molecule object Matter;Synthesize a variety of nutrients being easily utilized such as a variety of organic acids, enzyme, physiological activator;It takes up space, competes food, suppression The growth and breeding of the miscellaneous bacterias such as harmful bacteria processed, pathogen.
Bacillus megaterium:There is organophosphor in good degradation soil, improve and extend fertilizer efficiency, reduce chemical fertilizer Usage amount;Promote plant growth, pre- disease prevention;Improve the soil restoration ecological balance;Quality of agricultural product is improved, which preserves for a long time Non-inactivation.
Bacillus licheniformis:Main function is to contain antibacterial substance in metabolite, can inhibit the numerous of pathogen in soil Invasion to root with it are grown, the soil-borne disease of plant is reduced, prevents the generation of multiple diseases;The emergence rate of seed is improved, is carried High crop survival rate;Promote root growth;Improve soil aggregate structure, improve soil, improve soil water storage, energy storage capability, have Effect increases low temperature, alleviates continuous cropping obstacle, humus is synthesized again after promoting the organic matter decomposition in soil, greatly improves soil fertilizer Effect promotes plant growth, promotes early ripening of the crops.
Bacillus acidi lactici:With immunoregulation effect, there is inhibiting effect to pathogenic bacteria.
It is specific with reference to one in order to carry out apparent explanation to above-mentioned multi-functional enzyme liquid and preparation method thereof Embodiment illustrate, however, it should be noted that the embodiment merely to be better described the present invention, is not formed The present invention is improperly limited.
First, the mechanism of action of ferment bacterium is as follows:Ferment bacterium is typical aerobic fermentation micropopulation, metabolism production Containing tens kinds of different types of enzymes in object, such as amylase, protease, lipase, cellulase, oxidoreducing enzyme, lactase, wheat Bud carbohydrase, invertase, urase.The enzyme of these high activities can decomposition of organic matter in a short time, be rich in more particularly to decompose The sawdust of lignin and cellulose, sawdust, stalk, bark etc..It is to the crops mechanism of action:Crop root system vigor is improved, Increase absorption area;Improve plant nutrition situation, ensure crop robust growth;Increase chlorophyll content, increase leaf-area coefficient, Enhance photosynthesis;Respiratory intensity is reduced, reduces nutrient consumption, ensures substance accumulation;Plant growth is stimulated, is had anti-well Disease effect.
The multi-functional enzyme liquid that the present invention prepares has following functions effect:
1st, photosynthesis enhances, and promotes nutrient absorption, and agricultural product fruit size and density increase, and can increase production 20%- 30%;
2nd, it is easy to use, greatly reduce the usage amount of chemical fertilizer and pesticide, planting cost declines 30%;
3rd, soil is improved, improves soil property, increases fertility of land, resists hardened, anti-continuous cropping, continuous cropping cultivation is accessible;
4th, enhance crop immunity, prevent various pest and disease damages, decompose the Harmful Residue in agricultural product, improve food security Property;
5th, quality of agricultural product is improved, extends the agricultural products fresh-keeping phase, taste is naturally, pure in mouth feel;
6th, it is environmentally protective, it is pure natural, it is harmless to people and animals and environment.
The enzyme liquid can improve soil nutrient content, improve soil physical and chemical property, improve plant nutrient, and it is micro- to improve soil Biota improves soil activation, due to the increase of Soil Microorganism quantity, improves plant nutrient, and soil porosity increases, Accelerate the decomposition of soil nutrient, increase the microbe metabolite such as secretion such as micro-element, amino acid, auximone, so as to living Change soil and improve fertility.
Its general physical and chemical index meets the regulation of table 1;Characteristics index meets table 2 using 3 or 3 or more indexs Regulation.
The 1 general physical and chemical index of planting industry plant enzyme of table
Project Index
PH value≤ 6.0
Thick many candies g/L >= 20
Organic acid, (in terms of lactic acid), g/L (liquid), >= 1
Living bacteria count, CFU/g (mL) >= 107
Table 2 planting industry plant enzyme characteristics index
Project Index
Micro- a g/L >= 2
Amino acid g/L >= 10
Oligosaccharide g/L >= 5
Protease U/L >= 100
Cellulase mg/g/24h >= 30
Invertase mg/g/24h >= 1000
Dextranase U/L >= 200
Oxidoreducing enzyme mg/g/24h >= 15
Auxin IAA ng/mL (g) >= 15
Polyphenol g/L >= 0.1
Organic matter g/L >= 1
Specifically, it is as follows to obtain application method in different crops:
First, usage and dosage;
1st, matching method:100 times of dilutions are+6 bottle cap of=1 sprayer.+ 4 bottle cap of 200 times=1 sprayer.300 times= + 2 bottle cap of 1 sprayer.And so on.
2nd, it is manured into soil:Be diluted with water 200 times spray or be sufficiently stirred with farm manure, organic fertilizer etc. after apply soil Earth.
3rd, foliage-spray:300 times are diluted with water to spray.
4th, the prevention and control of plant diseases, pest control:100 times are diluted with water to spray on the crop for have pest and disease damage.
5th, dosage:Field crop, every season, (bread crop, plantation to harvesting) used 3-4 liters per acre.Booth vegetable, fruit Wait industrial crops dosage that can increase.Different Crop, different regions, different methods of cultivation etc., dosage is also different.It should be according to practical feelings Condition adjusts at any time.
2nd, fruit tree kind;
(1), it blooms preceding fruit (peach, plum, Lee, apricot, pears, cherry etc.):
(1), 200 times of dilutions are every perfusion fertilising in 15 days 1 time, totally 2 times after falling leaves.Perfusion fertilising 1 before second year germination It is secondary.
(2), before early spring blooms, 300 times of liquid are fully sprayed on branch, trunk, and are manured into soil after being mixed with farm manure.
(3), it spends and falls behind to before growing 5-6 piece leaves, 300 times of dilution foliage-spray and perfusion fertilising are used.
(4), the every 10 days 1 time foliage-spray of 300 times of dilutions after result, totally 3 times.
(5), the generation of pest and disease damage in order to prevent carries out 1 leaf in every 7 days before pest and disease damage occurs with 100 times of dilution Face sprays, totally 3 times.
(6), 20 days before ripe harvest time, foliage-spray is carried out with 300 times of dilutions.
(2), come into leaves preceding fruit (Malus spectabilis, jujube tree, pomegranate, hawthorn, grape, persimmon, apple, persimmon, grape, core Peach, citrus, Chinese chestnut etc.):
(1), 200 times of dilution applied fertilizer every perfusion in 15 days after falling leaves, totally 2 times.Perfusion fertilising 1 before second year germination It is secondary.
(2), 5,6 young leaves are grown to before blooming, are applied fertilizer with 300 times of dilution foliage-sprays and perfusion.And and farmers' It is manured into soil after fertilizer mixing.
(3), it spends to fall behind and 1 foliage-spray was carried out every 10 days with 300 times of dilution, totally 3 times.
(4), 300 times of dilutions progress in every 10 days, 1 foliage-spray after result, totally 3 times.
(5), the generation of pest and disease damage in order to prevent carries out 1 leaf in every 7 days before pest and disease damage occurs with 100 times of dilution Face sprays, totally 3 times.
(6), 20 days before ripe harvest time, foliage-spray is carried out with 300 times of dilutions.
3rd, greengrocery;
(1) fruit type vegetables (capsicum, cucumber, tomato, cucurbita pepo, eggplant, fresh kidney beans, watermelon, muskmelon, muskmelon, strawberry Deng):
1st, sowing is pulled out after seed is impregnated 1 hour in 200 times of dilution.
2nd, soil is carried out with 300 times of dilution on seedling plate and pour fertilising 2 times, uniformly pour.
With being transplanted after 300 times of dilution root dippings and fertilising is perfused when the 3, transplanting.
4th, after transplanting 15 1 foliage-spray is carried out with 300 times of dilution within every 10 days, totally 3 times.
5th, the pest and disease damage generation phase carries out 1 foliage-spray in every 7 days with 100 times of dilution, totally 3 times.
6th, 15 days before harvest time, foliage-spray is carried out 1 time with 300 times of dilutions.
(2), leafy vegetable (romaine lettuce, Chinese cabbage, leek, green onion, cabbage, rape, celery etc.):
1st, seed pulls sowing out after being impregnated 1 hour in 200 times of dilution.
2nd, soil is carried out with 300 times of dilution on seedling plate and pour fertilising 2 times, uniformly pour, every square metre of use 300ml。
It is transplanted and is perfused with after 300 times of dilution root dippings when the 3, transplanting.
4th, after transplanting 15 1 foliage-spray is carried out with 300 times of dilution within every 10 days, totally 3 times.
5th, the pest and disease damage generation phase carries out 1 foliage-spray in every 7 days with 100 times of dilution, totally 3 times.
6th, 15 days before harvest time, 300 times of dilution carries out foliage-spray 1 time.
(3), rhizome vegetable (garlic, onion, ginger, radish, carrot, asparagus lettuce, potato, pachyrhizus etc.):
1st, sowing is pulled out after seed is impregnated 1 hour in 200 times of dilution.
2nd, soil is carried out with 300 times of dilutions on seedling plate and pour fertilising 2 times, uniformly pour.
It is transplanted and is perfused with after 300 times of dilution root dippings when the 3, transplanting.
4th, after transplanting 15 1 foliage-spray is carried out with 300 times of dilution within every 10 days, totally 3 times.
5th, the pest and disease damage generation phase carries out 1 foliage-spray in every 7 days with 100 times of dilution, totally 3 times.
6th, 15 days before harvesting, 300 times of dilutions spray 1 time.
4th, bread crop (rice, wheat, millet, corn and soybean, small coarse cereals etc.);
1st, sowing is pulled out after seed is impregnated 1 hour in 200 times of dilution.
2nd, soil is carried out with 300 times of dilution to pour 2 times, uniformly pour on seedling plate,
Every square metre uses 300ml.
3rd, broadcast sowing or rice transplanting before with 300 times of dilution carry out 1 soil-perfusion apply fertilizer, about using 200ml stostes.
4th, after transplanting 15 1 foliage-spray is carried out with 300 times of dilution within every 10 days, totally 3 times.
5th, the pest and disease damage generation phase carries out 1 foliage-spray in every 3 days with 100 times of dilution, totally 3 times.
5th, medicinal crop (ginseng, the root of straight ladybell, campanulaceae, Chinese medicine etc.);
1st, sowing is pulled out after seed is impregnated 1 hour in 200 times of dilution.
With 300 times of dilutions be once perfused when the 2nd, transplanting and apply fertilizer, about 200ml stostes.
3rd, after transplanting 15 1 foliage-spray is carried out with 300 times of dilution within every 10 days, totally 3 times.
4th, the phase occurs for pest and disease damage, and 1 foliage-spray is carried out within every 7 days with 100 times of dilution of dilution, totally 3 times.
5th, 2 years or more growth periods, repetition do the 3rd, 4.
6th, flowers, nursery stock, sunflower, rape etc.;
1st, it soaks seed, 200 times of dilutions impregnate 1 hour.
2nd, seedbed is applied fertilizer, and 300 times of dilutions carry out 2 fertilisings, is applied fertilizer before transplanting with 300 times of dilution perfusion.
With 300 times of dilution root dippings when the 3rd, transplanting, 1 blade face was carried out every 15 days with 300 times of dilutions after transplanting 15 It sprays, totally 3 times.
4th, the phase occurs for pest and disease damage, and 1 foliage-spray was carried out every 5 days with 100 times of dilutions, totally 3 times.
5th, 15 days before harvesting, foliage-spray is carried out with 300 times of dilutions.
7th, tealeaves;
1st, 300 times of dilutions of sprinkling before germination, offer the full penetration of branch bar tea, send out gap twice and are also fully sprayed by this method It spills.
2nd, it is primary at interval of sprinkling in 10-15 days after tea tree germination, after current year tealeaves harvests, in order to which second year is had a good harvest Continue sprinkling 2 times.
3rd, the phase occurs for pest and disease damage, and 1 foliage-spray was carried out every 3 days with 100 times of dilutions, totally 3 times.
8th, tobacco leaf;
1st, tobacco leaf seed is sowed after being impregnated 2 hours with 200 times of dilutions.
2nd, it is sprayed with 300 times of dilutions in tobacco leaf nursery 1~2 time (situations such as tobacco leaf is suffered from malaria after prevention nursery).
With 300 times of liquid root dippings when the 3rd, transplanting, at interval of 15 days after transplanting, with 300 times of dilutions sprinklings 1 time, totally 2~3 It is secondary.
4th, the phase occurs for pest and disease damage, and 1 foliage-spray is carried out within every 7 days with 100 times of dilutions, totally 3 times.
5th, 500 times of dilution foliage spray 1 time before harvesting.
9th, fruits and vegetables, flowers etc. are fresh-keeping;
Effect:At normal temperatures, leaf vegetables can prolong storage period 3-5 days, and melon and fruit class can prolong storage period 6-10 days.
Application method:Before fruits and vegetables storage, this product dilution is uniformly sprayed for 300 times on surface.
After " more objects " ferment composite fertilizer is diluted with 200 times of water when the 1st, making farm manure, equably stirred with farm manure It mixes, is used after fermentation.
2nd, Manufactured farm manure:After 200 times of the water dilution of " more objects " ferment composite fertilizer, with Nong Jia Fei Zhuo it is even into Row stirring, you can use.
3rd, various agricultural wastes, food waste offal etc. can use waste material, with 200 times of dilutions of " more objects " agricultural composite enzyme It stirs evenly, is used after fermentation.
The beneficial effects of the invention are as follows:
1st, enzyme liquid provided by the invention accelerates the corruption of plant stalk etc. in soil by microbial activities, and then improves The soil organism.
2nd, enzyme liquid provided by the invention is used for agricultural planting, can effectively reduce destruction of the heavy metal to soil, preferably Land resource is protected, avoids Environment pollution.And it containing multiple nutritional components, preferably meets needed for crop growth Element improves the output value yield of agricultural planting, it is ensured that the economic benefit of agricultural planting.
3rd, enzyme liquid provided by the invention is used for agricultural planting, and pol, weight and the size of raise crop greatly carry Height, and reduce agricultural chemicals expense, fertilizer cost and labour cost, reduces cost, and the salt in soil prevents continuous cropping obstacle without accumulation, point Remains of pesticide is solved, so non-environmental-pollution, apparent to the improved effect of soil, particularly various growth is hindered and disease has Preferably prevent and prevent from acting on.
4th, enzyme liquid is prepared using method provided by the invention, processing method is simple, is easy to grasp, and production process is easy to steady Fixed control, has preferably ensured the quality of agricultural planting enzyme liquid fertilizer.
Particular embodiments described above has carried out the purpose of the present invention, technical solution and advantageous effect further in detail Describe in detail it is bright, it should be understood that the above is only a specific embodiment of the present invention, the guarantor being not intended to limit the present invention Range is protected, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this Within the protection domain of invention.

Claims (8)

1. a kind of preparation method of multi-functional enzyme liquid, which is characterized in that including:
Kuh-seng, Phytolacca acinosa or rice chaff and sugar are added in container according to the ratio of mass ratio 1: 0.7-1 by S101, and with weight pressure System is extracted, and obtains medicinal herbs extract liquor;The medicinal herbs extract liquor and water are mixed with the ratio of mass ratio 1: 1.5-2, fermentation Afterwards, medicinal herbs zymotic fluid is obtained;
The obtained medicinal herbs zymotic fluids of S101 and culture solution are added in the first fermentation tank by S102 according to the ratio of mass ratio 1: 70-110 In, it cultivates 5-9 days, the sieve for crossing 65-70 mesh obtains medicinal herbs zymotic fluid culture solution;
Bacillus and culture solution are added according to the ratio of mass ratio 1: 60-100 in the second fermentation tank, cultivate 5-9 by S103 My god, the sieve for crossing 60-65 mesh obtains bacillus culture solution;
S104, the bacillus for by ratio of weight and the number of copies obtaining medicinal herbs zymotic fluid culture solution that 1 part of S102 is obtained, 1 part of S103 are trained Nutrient solution, 0.00166-0.0144 parts of trace element salts add in mating groove, and concentrated machine concentration, obtains concentrate after mixing;
S105, the concentrate that S104 is obtained is in 1.5Kg/cm2-3Kg/cm2Under the conditions of carry out homogeneous, fermentation, and by homogeneous, hair After liquid after ferment crosses the sieve of 100-120 mesh, multi-functional enzyme liquid is obtained.
2. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that the bacillus includes: Bacillus subtilis, bacillus megaterium, bacillus licheniformis or Bacillus acidi lactici.
3. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that extraction temperature in step S101 It it is 20 DEG C -60 DEG C, the fermentation temperature of the medicinal herbs extract liquor is 20 DEG C -40 DEG C.
4. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that the institute in the step S102 State that the cultivation temperature in the first fermentation tank is 36 DEG C -38 DEG C, oxygen intake is 0.35-0.55 cubes m/h.
5. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that the institute in the step S103 State that the cultivation temperature in the second fermentation tank is 30 DEG C -38 DEG C, oxygen intake is 0.4-0.58 cubes m/h.
6. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that the institute in the step S104 Trace element salt is stated to be made of 0.0008-0.0014 parts of borates and 0.00003-0.00004 parts of molybdates by ratio of weight and the number of copies.
7. the preparation method of multi-functional enzyme liquid according to claim 1, which is characterized in that dense in the step S104 Contracting condition is pressure 600mmHg-750mmHg, -75 DEG C of temperature 60 C.
8. a kind of multi-functional enzyme liquid, which is characterized in that the multi-functional enzyme liquid is by any one of described claim 1 to 7 The preparation method of the multi-functional enzyme liquid is made.
CN201810069874.0A 2018-01-24 2018-01-24 A kind of multi-functional enzyme liquid and preparation method thereof Pending CN108129238A (en)

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Publication number Priority date Publication date Assignee Title
CN109220463A (en) * 2018-09-21 2019-01-18 大连市竹庭休闲农业有限公司 A kind of cherry implantation methods
CN110627568A (en) * 2019-10-21 2019-12-31 河北省农林科学院石家庄果树研究所 Seed soaking composition and method for improving germination rate and seedling rate of cherry seeds
CN111019848A (en) * 2019-12-26 2020-04-17 郑州浩创农业科技有限公司 Disease-resistant functional microbial inoculum and preparation method thereof
CN111233542A (en) * 2020-02-23 2020-06-05 黄河三角洲京博化工研究院有限公司 Special fertilizer for improving quality of Chinese chives and preparation method thereof
CN114213191A (en) * 2021-11-23 2022-03-22 清大国杰生物科技有限公司 Agricultural folic acid active mycoprotein polypeptidase plant enzyme, formula and preparation method

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CN105254359A (en) * 2015-10-28 2016-01-20 山东宝来利来生物工程股份有限公司 Enzyme fertilizer and preparation method and application thereof
CN106064971A (en) * 2016-06-03 2016-11-02 北京多物科技有限公司 The preparation method of a kind of ferment compound fertilizer, ferment compound fertilizer and application thereof

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WO2007114324A1 (en) * 2006-03-31 2007-10-11 Menicon Co., Ltd. Method of treating biomass, compost, mulching material for livestock and agent for treating biomass
CN105254359A (en) * 2015-10-28 2016-01-20 山东宝来利来生物工程股份有限公司 Enzyme fertilizer and preparation method and application thereof
CN106064971A (en) * 2016-06-03 2016-11-02 北京多物科技有限公司 The preparation method of a kind of ferment compound fertilizer, ferment compound fertilizer and application thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109220463A (en) * 2018-09-21 2019-01-18 大连市竹庭休闲农业有限公司 A kind of cherry implantation methods
CN110627568A (en) * 2019-10-21 2019-12-31 河北省农林科学院石家庄果树研究所 Seed soaking composition and method for improving germination rate and seedling rate of cherry seeds
CN111019848A (en) * 2019-12-26 2020-04-17 郑州浩创农业科技有限公司 Disease-resistant functional microbial inoculum and preparation method thereof
CN111233542A (en) * 2020-02-23 2020-06-05 黄河三角洲京博化工研究院有限公司 Special fertilizer for improving quality of Chinese chives and preparation method thereof
CN114213191A (en) * 2021-11-23 2022-03-22 清大国杰生物科技有限公司 Agricultural folic acid active mycoprotein polypeptidase plant enzyme, formula and preparation method

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Application publication date: 20180608