JPS6272604A - Skin cosmetic - Google Patents

Abstract

PURPOSE:A skin cosmetic keeping the skin in a healthy state or restoring it to a healthy state by promoting water retention effect of the skin of its own, having improved skin beautifying effects, obtained by blending a skin cosmetic with sterol glycoside prepared by extracting a plant. CONSTITUTION:A skin cosmetic is blended with preferably 0.1-5wt% based on its total amounts of a sterol glycoside such as sitosteryl-beta-D-glycoside, etc.,) shown by the formula, which occurs in leaves, petioles, roots, seeds, etc., of unpolished rice, soybean, Tetragonia tetragonoides(Pall) 0. Kuntze, potato, alfalfa, etc., and obtained by extracting, separating and purifying a lipid from the tissue by a conventional procedure, to give a skin cosmetic which normalizes tissue ranging from the lamellated granulation of the top of stratum granulosum to the corneum, promotes water retention ability of the skin of its own to improve dried skin, keeps the skin in a healthy state to prevents its aging and has beautifying effects to provide the skin with wetting properties, flexibility and elasticity.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of the Invention) The present invention relates to a skin cosmetic containing styrene glucoside that can restore the moisture retention function of the skin.

(Prior art) In order to maintain healthy skin, hydrophilic skin moisturizers and oily skin softeners that provide appropriate moisture and oil content to the skin have traditionally been blended into skin cosmetics. .

Skin moisturizers include glycerin, propylene glycol,
Polyethylene glycol, pyrolidone carboxylate, etc. are used, but these can absorb moisture from the stratum corneum, the outermost layer of the skin, and cause moisture loss in the skin. However, skin cosmetics that contain a large amount of these substances are not always satisfactory, as they give an unpleasant feeling such as stickiness.

In addition, liquid paraffin, petrolatum, olive oil, squalane, lanolin, synthetic ester oils, etc. are used as skin softeners, but these are also included in skin cosmetics in such amounts that they sufficiently prevent water evaporation from the epidermis. When exposed to skin, it has disadvantages such as inhibiting the normal metabolism of the skin.

(Disclosure of the Invention) In view of the above-mentioned shortcomings and drawbacks of skin moisturizers and skin softeners, the present inventors have determined that the physical effects of these ingredients can be used to replenish moisture to the epidermis or prevent water evaporation from the epidermis. The result of intensive research aimed at providing skin cosmetics that maintain or repair the skin in a healthy state by restoring the skin's inherent moisture retention function, rather than relying on it. The present invention was completed based on the discovery that a skin cosmetic containing styrene glucoside exhibits an effect that meets the above objectives.

Water in the skin is distributed from the dermis to the basal cell layer of the epidermis, and then to the keratin layer.
Moisture always moves from the inside of the skin to the outer layer along the gradient of water content, which decreases toward the outer layer of the skin, and transpires to the outside through the stratum corneum, but this water evaporation mainly occurs in the lamina at the top of the granular layer. It is controlled by a protective function (barrier function) consisting of dense cell tissue ranging from granules to the stratum corneum, and the amount of transpiration [Transepidermal Wa
ter 1oss ) value (hereinafter referred to as TWL value)
) is 0.2 to 0.3 Q / cd / on the skin surface of the forearm under normal conditions of healthy skin.
h r (7) Range, normal! ,tO,2511i1/
The cti/hr level is maintained below about
Alternatively, in dry skin such as aging skin, the TWL value shows the upper limit of the above range or a value larger than the upper limit depending on the degree of dryness, and it is recognized that the moisture retaining function of the skin is reduced. This is because, in the case of dry skin, the normal control limit of the protective function of the stratum corneum is exceeded, or the protective function is weakened.

Therefore, if the structures of the stratum corneum and lamellar granules can be densified and their defense functions activated, the water retention function of the skin will be restored and the skin will be maintained in a healthy state. At the same time, dry skin can be further improved or repaired. Therefore, the present inventors conducted intensive research on the effects of styrene glucoside, which is known to be present in plant leaves, petioles, seeds, and seeds, on human skin. By normalizing the tissues from the top granules at the top of the layer to the stratum corneum and restoring the skin's own moisture retention function, it improves dry skin or maintains the skin in a healthy state to prevent aging. , moisture to the skin (moist feeling), flexibility (°smooth feeling)
It has been found that it has a beautifying effect on the skin by imparting elasticity and luster.

In the case of the skin cosmetics of the present invention, unlike conventional skin cosmetics, there is no need to incorporate large amounts of the above-mentioned skin moisturizers and skin softeners, and there is no risk of interfering with the normal physiological functions of the skin.

(Objective of the Invention) The object of the present invention is to maintain or repair the skin in a healthy state by restoring the skin's inherent moisture retention function, and to provide a skin cosmetic that has excellent skin beautifying effects. It is on offer.

(Structure of the Invention) The present invention is a skin cosmetic characterized by containing styrene glucoside extracted from plants.

(Explanation of specific structure) Styrene glucoside used in the present invention is known to be present in plants, petioles, roots, parapets, etc., and can be obtained by extracting, separating and purifying the above-mentioned tissues using ordinary lipid extraction methods. It is being [Oil Chemistry, Vol. 20, No. 10, No. 85-97
Page, 1971.

Journal of the Japanese Society of Agricultural Chemistry, Volume 47, No. 3, Nos. 229-23
0 page, 1973, etc.] Also, the examination (Tsurna, Tetr
styrene glucoside (β-5itost) obtained by extraction from Agonia tetragonides).
It has been reported that eryl-β-D-glucoside, etc.) has an inhibitory effect on restraint water immersion stress ulcers in mice. [Refer to Pharmaceutical Journal, No. 103.8, No. 1, pp. 43-48, 1983] The present inventors have investigated the use of rice grains containing particularly high styrene glucosides or from which it is easy to extract styrene glucoside, such as brown rice and soybean. , examination, styrene glucosides were extracted, separated and purified from plants such as potatoes and alfalfa as described in the experimental examples described below, and the styrene glucosides were blended into various skin cosmetics to be used in the various manners described below. A test was conducted.

(Experimental Example) Experimental Example (1)...See Table 1 ■ 10 kg of brown rice (Koshihikari from Akita) was ground to obtain brown rice powder with a particle size of 0.1 to 0.5M.

(2) This powder was extracted three times with 1001 chloroform-methanol mixture (chloroform:methanol = 2:IV/V, hereinafter simply abbreviated as 2:1) at a temperature of 50-60''C while stirring.

(2) This extract was washed with water three times using the Folch method, and the chloroform layer was concentrated under reduced pressure to obtain 10 g of a total lipid solution in chloroform. (Calculated amount of total lipids: 300 g) ■ This solution was injected into a column containing 1 kg of silicic acid, and again 101 chloroform and acetone were injected in sequence, and the acetone eluate was concentrated to obtain glycolipid section 309.

■ Next, this glycolipid was subjected to silicic acid column chromatography, and a chloroform-acetone mixture (7.5:2.5
The eluate of ~6.5:8.5) was concentrated to obtain 7 g of crude styrene glucoside.

■ This crude styrene glucoside was further subjected to silicic acid column chromatography, treated with a chloroform-methanol mixture (8:2) to remove impurities, and then eluted with a chloroform-methanol mixture (7:3). The eluate was collected and concentrated, chloroform was distilled off under reduced pressure, and purified.
4.59 of the desired styrene glucoside was obtained.

The styrene glucoside obtained by the above extraction is
The main component is β-sitosteryl β-D-glucoside, which is expressed by the Mizozo formula in the table, and stigmasteryl β-D-
It contained glucoside, Δ7-stikmasteryl β-D-glucoside, and contained 95.7 wt % (purity) of styrene glucoside 1.

The confirmation and purity of styrene glucoside was confirmed using gas chromatography mass spectrometer and nuclear magnetic resonance Fit5.
1 2 < Giant 11i-1 remaining
Residue No. 2 Table H β-sitosteryl β-D-glucoside Experimental Example (2) The experimental example (
6.7 g of the target styrene glucoside (purity 96.5 wt%) was obtained in the same manner as the extraction separation and purification method in 1).

The component of this styrene glucoside was β-sitosteryl β-D-glucoside, similar to the styrene glucoside of brown rice.

Experimental Example (3) From 10 kq of alfalfa whole plant dry matter, the experimental example (
The target styrene glucoside (purity 94.7 wt%) was extracted with 6.57 wt% in the same manner as the extraction separation purification method in 1).
Obtained.

The main component of this styrene glucoside is β-sitosteryl β-D-
It was a glucoside.

In the present invention, the blending amount of the styrene glucoside is 0.1 to 5% based on the total amount of the skin cosmetic (composition).
A range of wt% is preferred. The blending amount is 0.1w
Below t %, the effect is not fully achieved, while 5 w t
%, it cannot be expected to improve the effect commensurate with the increase.

The skin softening/sugar agent of the present invention can be applied to, for example, lotions, emulsions, creams, packs, etc.

In addition to the above, pigments, fragrances, preservatives, surfactants, pigments, antioxidants, and the like may be appropriately incorporated into the skin cosmetic of the present invention within a range that achieves the object of the present invention.

The present invention will be explained in detail below based on Examples and Test Examples.

The methods for measuring or evaluating the TWL value, rate of change in TWL value, angular PR/irl turnover rate, and exfoliation characteristics of corneocytes are shown below.

(1) TWL value A method was used to measure the humidity change in the air above the sealed skin surface over a certain period of time using electrical resistance.

That is, the test subject's skin surface is sealed in a measurement cell, forced dry air is vented into the cell to fully replace the inside of the cell with dry air, and then the ventilation of dry air is stopped and the measurement is carried out at that point. Determine the relative humidity RHs (%) in the cell, then leave it for 10 minutes and measure the relative humidity 'RHIO (%) in the cell again.
The TWL value was calculated from the humidity change at this time using the following formula.

However, Dt = Density of saturated water vapor in the air at measurement temperature (t''C) (g/l) V: Cell volume (A') S: Measurement area (d) (2) TWL value change rate The TWL values before and after applying the sample (skin cosmetic) to the skin were determined, and the rate of change was calculated using the following formula to evaluate the TWL reduction effect (moisture retention function enhancement effect).

TWL value before application of sample (skin cosmetic): TWLn TWL value after application of sample (skin cosmetic): TWLm (3)
Method for measuring the turnover rate of the stratum corneum: An ointment containing 5% by weight of the fluorescent dye danzyl chloride in white petrolatum is made, and the ointment is applied to the skin of the subject's forearm for 24 hours to inject danzyl chloride into the stratum corneum. osmotic bond. Thereafter, the test sample was applied to the same area twice a day (morning and evening), and the fluorescence of danzyl chloride was examined every day.The number of days until the fluorescence disappeared was defined as the turnover rate of the skin stratum corneum. Note that the normal turnover rate of the skin's stratum corneum is 1
It lasts for 4 to 16 days, but in aged skin it increases around 18 days. On the other hand, if the anti-aging effect appears, 1
It will be shortened to around 2 days.

(4) Peeling characteristics of keratinocytes Scotch tape (Tiban mending tape) was applied to the skin, and this was peeled off to allow the keratinocytes on the skin surface to adhere to the tape. Next, the condition of the keratinocytes was observed in detail using a scanning electron microscope, and the exfoliation characteristics of the keratinocytes were classified based on the criteria shown in Table 3, and their indices were determined.

Table 3 Criteria for Judging the Peeling Properties of Corneocytes The peeling properties of corneocytes serve as an index for determining the structural properties of the stratum corneum, and in general, in dry skin and aging skin, intercellular adhesion Is the resultant force weak and the structure less dense? It has been confirmed that the index becomes higher from -ki.

Examples 1 to 3 Comparative Example 1 [Skin Cream] Skin creams of Examples 1 to 3 of the present invention and Comparative Example 1 were prepared by blending the styrene glucoside obtained in the above experimental example [(1) Composition (2) Preparation method ω component Each skin cream was prepared by heating and dissolving the ingredients (and) respectively at 80"C, mixing, stirring and cooling to 30"C.

Test Example 1 When the skin creams of the present invention of Examples 1 to 3 and the skin cream of Comparative Example 1 were applied, their effects on 'I'WL values and keratin cell exfoliation properties were investigated.

(1) Test method 60 healthy normal skinned JR test subjects (age 20-2)
3 groups (A, O and E) of 20 people each (5 years old female)
In addition, there were 60 test subjects (females aged 20 to 25 years old) with normal dry skin in 3 groups of 20 each (B).

D and F groups).

Prior to the test, the TWL values on the skin surfaces of the left and right bent arms of all subjects were measured, and the average value was calculated for each group. Next, the skin cream of Comparative Example 1 was applied to the skin surface of the forearm of the test subjects, and the skin cream of Comparative Example 1 was applied to the left forearm for all the test subjects, and the skin cream of Example 1 and C was applied to the right forearm of each group. The skin cream of Example 2 was applied to Groups D and D, and the skin cream of Example 3 was applied to Groups E and F for one month continuously twice a day (morning and evening), and the cream was applied to all subjects on the day after the final application. The TWL value of the left and right forearm skin surface areas to which the product was applied was measured, and the keratin cell exfoliation properties were further evaluated.

After the application test, a doctor diagnosed no abnormalities in the skin or physical condition of the forearm of any of the test subjects.

(2) Results of skin cream application and TWL values after application.
Table 4 shows the TWL value change rate determined from the WL value and the index of keratinocyte exfoliation properties (all values are average values of 20 people in each group).

From the results shown in Table 4, Example 1 of the present invention,
The effects of skin creams 2 and 3 were observed.

■ In the normal, healthy skin of groups A, O, and E, the moisture retention function of the skin is originally maintained in a normal state. ) Although the effect of coating is difficult to become apparent, a slight improvement is still observed compared to Comparative Example 1.

■ For dry skin in groups B, D and F, the skin surface of the right forearm coated with the skin cream of the present invention (Examples 1, 2 and 3) was applied to the skin surface of the left forearm (comparative example 1). A significant improvement in T'WL value was observed compared to skin cream), and the value was equal to or approaching that of healthy skin.

Example 3: Comparison between creams of the present invention
In Examples 2 and 1, the water/minute retention function improvement effect was the highest, followed by Examples 2 and 1. Furthermore, similar results were obtained in the corneum cell peeling test.

That is, the skin creams of Examples 1, 2, and 3 of the present invention exhibited the effect of maintaining healthy skin in Groups B, C, and E;

It is clear that groups D and F have the effect of bringing the skin closer to healthy skin.

From these results, the styrene glucoside contained in the skin creams of Examples 1, 2, and 3 of the present invention effectively acts on epidermal cells, improves the intercellular bonding ability of the stratum corneum, and
It can be seen that the structure is made denser and the water retention function of the skin is enhanced, and that it is highly safe and does not interfere with the physiological functions of healthy skin. -1・ya1・ (hereinafter referred to as white) Test Example 2 TWL values and stratum corneum turnover rates of healthy skin and aged dry skin for the skin cream of the present invention of Example 3 and the skin cream of Comparative Example 1. We investigated the effect on

(1) Test method In the G group, 20 test subjects (females aged 20 to 25 years old) with normal healthy skin, and in the ■ group, 20 test subjects with dry skin due to aging ( For a total of 40 women (50 to 60 years old), the TWL values on the skin surface of the left and right forearms were measured in advance, and then ointment containing danzyl chloride was applied as an occluded patch on the left and right forearms for 24 hours.

Next, the skin cream of Comparative Example 1 was applied to the skin surface of the left forearm of all subjects in Groups G and H, and the skin cream of Example 1 was applied to the skin surface of the right forearm twice a day (in the morning, Evening) The solution was applied continuously for one month, and the fluorescence was examined every day. On the day after the final application, the 'I'WL values of the skin surface areas of the left and right forearms to which the cream was applied were measured for all test subjects.

After the completion of the application test, no abnormality was observed in the skin of the forearm or physical condition of all the test subjects according to the results of diagnosis by a doctor.

(2) Results TWL values before and after skin cream application (average value of 20 people in each group), TWL calculated from these TWL values
Table 5 shows the value change rate and the stratum corneum turnover rate (average values for 20 people in each group).

(Hereinafter referred to as prize) From the results shown in Table 5, the effects of the skin cream of Example 3 of the present invention were recognized as follows.

■ Since the functional status of the skin was maintained normally in the healthy normal skin of Group G, it was confirmed that the skin cream of the present invention (Example 3) restored the turnover rate of the stratum corneum. I couldn't. Further, regarding the TWL value, the same results as in Test Example 1 were obtained.

■ In the aged dry skin of group H, the skin surface of the right forearm to which the skin cream of the present invention (Example 3) was applied was compared to the skin surface of the left curved arm (to which the skin cream of Comparative Example 1 was applied). A normalization (shortening) of the turnover of the stratum corneum was observed, and the value approached that of normal skin. T
Regarding the WL value, application of the cream of the present invention (Example 3) showed a significant improvement (reduction in TWL value), similar to what was observed in normal dry skin in Test Example 1, and it was found that it was equivalent to the value of a normal person. Or something close to that value.

That is, it can be said that the skin cream of Example 1 of the present invention brings the turnover rate of the stratum corneum of aged dry skin closer to that of healthy skin, and enhances the water retention function.

Test Example 3 The skin creams of Comparative Example 1 and Examples 1 to 3 were applied to each of 30 elderly test subjects (women aged 26 to 34) complaining of dry skin twice a day (in the evening) for one consecutive month. . As a result of the diagnosis by the doctor, no abnormality was observed in the skin or physical condition of all the test subjects.

Moisture (moist feeling) on the skin after applying the cream,
Table 6 shows the results of a survey on all test subjects regarding the effects on flexibility (smooth feel), elasticity, and gloss.

As is clear from the results in Table 6, the skin creams of Examples 1 to 3 containing styrene glucoside were the same as those of Comparative Example 1.
Q6 Table Chest 7 Examples 4 to 5, Comparative Example 2 [Skin Lo Silane (Two-layer type)] Similar to Example 1, the styrene glucoside obtained in the above experimental example was blended. Each skin low silane was prepared and various tests were conducted.

(1) Composition (2) Preparation method After uniformly dissolving each component (A) and 03),
(2) and component (2) are mixed, stirred and dispersed, and then filled into a container.

When using, shake and disperse the contents evenly and apply to the skin.

Preview Example 4 The skin lotion silanes of Comparative Example 2 and Examples 4 to 5 were given to 30 test subjects (women aged 26 to 34 years old) who complained of dry skin twice a day (morning and evening) in a row. I applied it for a month. Diagnosis by a doctor revealed no abnormalities in the skin or physical condition of all subjects.

Next, Table 7 shows the results of a survey on all test subjects to determine the effect of applying Skin Losilane on the skin, which imparts moisture (moist feeling), flexibility (smooth feel), elasticity, and luster.

As is clear from the results in Table 7
All Skin Lolicons No. 5 showed better results than Comparative Example 2, but in particular, Skin Lolicine 3 of Example 5 showed a remarkable skin improvement effect.

(Effects of the Invention) The skin cosmetics containing the styrene glucoside of the present invention maintain or repair the skin in a healthy state by enhancing the moisture retention function that skin+i inherently has. The present invention provides a skin cosmetic that imparts moisture, flexibility, elasticity and luster to the skin, and has an excellent skin beautifying effect.

Claims (1)

[Claims] A skin cosmetic characterized by containing styrene glucoside extracted from plants.