JPS594276Y2 - electrophoresis box - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to an electrophoresis box of an automatic electrophoresis apparatus used for testing serum white matter.

Conventionally, electrophoresis has been used for testing serum proteins in clinical laboratories and the like.

Tests for serum proteins using this electrophoretic method were performed manually, such as applying serum onto a support made of cellulose acetate paper, and performing electrophoresis in an electrophoresis box. .

For this reason, the work is inefficient, and when measuring multiple samples, an extremely long work time is required.

In order to eliminate such drawbacks of the conventional electrophoresis method, an apparatus has been developed in which each step of this electrophoresis method is completely automated and all steps are performed continuously.

Among these, in the electrophoresis box in which the sample is subjected to electrophoresis, the support coated with serum is carried into the device by an endless belt placed inside the electrophoresis box, and is held at a predetermined position in order to energize the support. In this stopped state, electricity is applied for a predetermined period of time, and after electricity is applied, the belt is operated again to move the support to the next step.

That is, it has a structure as shown in FIGS. 1 to 3.

In these figures, 1 is the electrophoresis device main body, 2 is the lid, and 3 is the electrophoresis device body.
are the inlet 1a for feeding the support into the main body 1 and the electrophoresis composite body 1.
A pair of rollers are arranged near the exit 1b for carrying out the product, 4 is an endless belt hung on the rollers,
5 is a motor, and by rotating this motor 5, the roller 3 is rotated, and thereby the endless belt 4 is rotated.
Like driving.

Therefore, when a support is inserted through the inlet 1a, the support is fed into the main body 1 by the belt 4, and the support, which has been energized by the means described later, is again sent out from the outlet 1b by the belt 4.

Further, 6 is a partition wall fixed to the main body 1 or formed integrally with the main body 1, and the container-shaped portions IC, l separated by this partition wall.
Buffer solution 7 is placed in d.

Reference numeral 8 denotes a pair of filter paper stands provided on the left and right sides, which are shaped like long plates, and their upper surfaces are inclined as seen from the cross-sectional shape shown in FIG.

Reference numeral 10 denotes a support arm, to which springs 11 are fixed at both ends, and the springs 11 pinch screws 9 fixed to the filter paper base 8, thereby holding the filter paper base 8 in a detachable manner.

Reference numeral 12 is a column supporting the support arm 10 and is attached to the main body 1 so as to be able to move up and down.

Reference numeral 13 denotes cams fixed to the shaft 14. Two cams are provided at the front and rear so as to be located under the column 12. Rotation of the cam 13 causes the column 12, and therefore the filter paper table 8, to move up and down.

Reference numeral 15 has one end placed on the top surface of the filter paper stand 8, the other end of the filter paper soaked in the buffer solution 7, and 16 a long plate-shaped filter paper holder placed above each filter paper stand and fixed thereto. pin 1
6a is inserted into a groove 1e formed in the main body 1 to hold it at a constant height as shown.

17 is a wire stretched almost above the belt 4 by a fixture 18 fixed to the filter paper table 8; 19 is a shielding plate provided so as to be able to slide up and down to open and close the inlet 1a and the outlet 1b; 20 is a crank mechanism for moving the shielding plate 19 up and down based on the rotation of the shaft 14.

Next, the operation of this conventional electrophoresis chamber will be briefly explained.

First, a support coated with serum as described above is inserted from the base 1a by an appropriate means.

At the same time, the motor 5 is rotated, and the endless belt 4 for conveyance is rotated.
When the belt 4 is operated, the support body is fed into the main body by the belt 4.

When this support reaches a predetermined position, the operation of the belt is stopped, and then the shaft 14 is rotated by a suitable drive mechanism, and the cam 13 fixed thereto is rotated, as described above. The filter paper stand 8 is pushed up, and the upper surface of the filter paper stand 8 comes to be pressed against the lower surface of the filter paper presser 16.

Here, as shown in FIG. 2, if the distance between both belts is made narrower than the width of the support 30 coated with serum, the support 30 on the belt 4 that has been sent to a predetermined position will have both ends. It protrudes from the outside of belt 4.

Therefore, when the filter paper stand 8 is raised as described above, the support body 30 also rises at the same time, leaves the belt, and is sandwiched together with the filter paper 15 between the filter paper stand 8 and the filter paper presser 16.

When current is applied through an electrode (not shown) placed in the buffer solution 7, the support body 30 is energized through the filter paper 15, one end of which is soaked in the buffer solution 7.

On the other hand, when the shaft 14 is rotated, the shielding plate 19 is raised via the crank mechanism 20, so the cam 1
3, the filter paper stand 8 rises and at the same time the inlet 1a
and exit 1b are closed.

Next, when the cams 13 and 20 are rotated again after energization for a certain period of time, the filter paper table 8 and the shielding plate 19 are lowered.

At this time, as the filter paper table 8 descends, the fixture 1 fixed to it
Since the wire 17 stretched over the filter paper 8 also descends, even if the support adheres to the filter paper presser 16, the wire 17 also ensures that the support is lowered.

The support that has finished electrophoresis in this way is placed on the belt 4 again, and when the motor is rotated to operate the belt, the support is sent out from the outlet 1b and transferred to the next step.

In the conventional electrophoresis box described above, since the filter paper holder 16 is a flat plate, when the end of the support is sandwiched between the filter paper holder 8 and the filter paper holder 16, the filter paper holder 8 and the filter paper holder 16 are in contact with each other. Unless the surfaces have good flatness and both surfaces are in contact at all parts, there is a drawback that the contact between the support and the filter paper will be insufficient.

Furthermore, when the filter paper presser 16 is lowered to place the support back on the belt after energization, the support may stick to the filter paper presser 16 and not fall onto the belt.

Therefore, in the conventional example, it was necessary to stretch the wire 17 so that the support body could be peeled off from the filter paper presser 16 when the filter paper table 8 was lowered.

However, since it is not possible to provide a large distance between the belt 4 and the wire 17, there are also disadvantages in that the support may be caught in the process of being conveyed.

The present invention was developed in view of the above points, and the filter paper holder is made into a bar-shaped cross section with a circular or triangular cross section, and the support is sandwiched between the filter paper base and the filter paper holder, and the support is attached to the filter paper. To provide an electrophoresis box in which a filter paper presser can be easily separated from a support body while ensuring linear contact with the filter paper holder.

The specific contents of the electrophoresis box of the present invention will be explained below based on the illustrated embodiment.

In FIGS. 4 to 6, reference numeral 31 denotes a filter paper base made of a material such as glass, which has almost the same shape as the conventional example, and has guide shafts 32 fixed to both ends thereof.

The guide shaft 32 is then inserted into a groove 30C formed on the inner surface of the electrophoresis box body 30 and shaped as shown on the left side of FIG. placed.

Reference numeral 33 denotes a filter paper holder, which is rod-shaped and made of a glass material or the like, and its both ends are inserted into and supported by another groove 30d on the inner surface of the main body 30.

Reference numeral 34 denotes a filter paper stand holder, which is fixed to the slide shaft 35.

Further, this slide shaft 35 is arranged in a bearing 36 so as to be able to move up and down.

Reference numeral 37 denotes a drive pipe arranged to be able to move up and down, and is used to move the filter paper stand 31 up and down via the slide shaft 35 and the filter paper stand holder 34.

38 is a spring. The rest of the structure is substantially the same as the conventional example shown in FIGS. 1 to 3, so substantially the same parts are indicated by the same reference numerals as in the conventional example, and the explanation thereof will be omitted.

Next, the operation of the electrophoresis box of the present invention will be explained.

First, when the drive pipe 37 is lowered, the inlet and outlet shielding plates 19 attached to both ends of the pipe 37 are opened and the filter paper table 31 is lowered.

In this state, the support coated with the serum is fed through the inlet and further into the apparatus by the feed belt 4, and the belt is stopped at a predetermined position to stop the support from moving.

If the drive pipe 37 is raised here, the filter paper table 31 will be raised and the support will be separated from the belt.

When the drive pulp 37 further rises and the filter paper stand 31 is pushed up, the edge of the support is sandwiched between the filter paper stand 31 and the filter paper presser 33 at the position where the filter paper stand 31 and the filter paper presser 33 come into contact, and the support is lifted as it is. .

When the filter paper stand 31 rises further, the groove 3 formed in the main body 30
Since 0C has the shape shown in the figure, both corrugated paper bases 31 move diagonally upward in a direction away from each other.

As a result, both sides of the support are provided with a filter paper stand 31 and a filter paper presser 33.
Because they are held together and pulled outward, there is no slack in the center.

If the current is applied in this state, a good image will be formed since the current is applied without any sagging of the support.

Subsequently, when the drive pipe 37 is lowered after the energization ends, the filter paper stand 31 and the filter paper presser 33 are lowered while holding the support.

On the way, the filter paper holder stops descending to reach the lower end of the groove 30d.

Therefore, only the filter paper base 31 is lowered, and the support is naturally separated from the filter paper holder and placed on the belt due to its own weight and the adhesive force between the support and the filter paper base 31.

The filter paper table further descends diagonally inward and returns to its initial position.

At this point, the belt 4 is moved to carry the support out of the migration box.

When the end of the support is clamped between the filter paper base and the filter paper holder by raising the filter paper base, the support is in a straight line with the filter paper at all parts because the filter paper base is flat and the filter paper holder is rod-shaped. We will definitely come into contact.

Furthermore, when the filter paper table is lowered, the support body easily separates from the rod-shaped filter paper holder.

Therefore, the wire used in the conventional example is not required.

Furthermore, since the support naturally separates from the filter paper holder, no distortion occurs in the support at this time.

Note that the filter paper presser is not limited to a round rod shape, but may be a rod shape with a triangular or other polygonal cross section, with the corner portions facing downward.

Furthermore, a wire may be wound around a round rod as shown in FIG. 7, and a resin film may be wrapped around the rod for insulation.

As explained above, the electrophoresis box of the present invention ensures reliable contact between the support and the filter paper, so that good images can be obtained.

Furthermore, since the filter paper holder and the support are in linear contact, the support can be naturally separated from the filter paper holder by simply lowering the filter paper table after electricity is applied, and there is no need for a device to separate the support. Therefore, the electrophoresis device can be made into a simple structure, and the electrophoresis image will not be adversely affected by distortion of the support during separation.

[Brief explanation of drawings]

Figure 1 is a plan view of a conventional electrophoresis box, Figures 2 and 3 are
The figures are line II-II and line III- in Fig. 1, respectively.
4 is a partially cutaway plan view of the electrophoresis box of the present invention, and FIGS. 5 and 6 are sectional views taken along line III.
FIG. 7 is a cross-sectional view taken along lines v-V and vI-vI in the figure, and is a diagram showing an example of a filter paper presser. 30...Main body, 31...Filter paper stand, 33
...Filter paper holder, 34...Filter paper stand holder,
37... Drive pipe.

Claims (1)

[Scope of utility model registration request] A conveyor belt for carrying in and out the support coated with serum, and a pair of long plate-shaped plates that are normally located slightly below the conveyor belt, on which one end of the filter paper is placed, and that are movable up and down. a filter paper stand, and a filter paper presser disposed above the filter paper stand and having a cross-sectional shape capable of making linear contact with the support when the support is clamped; When performing this, the filter paper table is raised, the support is held between it and the filter paper presser, and the support is separated from the conveyor belt, and electricity is applied to the support through the filter paper, and after the energization is completed, is an electrophoresis box in which the filter paper table is lowered, the support is peeled off from the filter paper presser by the support's own weight and the adhesive force between the support and the filter paper table, and the support is placed on the conveyor belt. .