JPS5920847A - Apparatus for electrophoresis - Google Patents

Abstract

PURPOSE:To automate processes, by holding a supporting body by a receiving plate and a pushing plate, positively separating the supporing body by a supporting body separating means which is provided on the pushing plate when the supporting body is separated and conveyed, thereb making it possible to separate and convey the supporting body in an electrophoresis box. CONSTITUTION:When a conveying table 21 completely enters into an electrophoresis box 50, rods 69 and 76 are lifted by cams 71 and 77, and a filter paper receiving plate 64 and an application receiving plate 30 are lifted. Then, the supporting body is held by the filter paper receiving plate 64 and the receiving plate 67, and the application of serum is performed by a serum applying tool 40. After the application of serum, the rod 76 is lowered by the cams 71 and 77 again. When the conveying table 21 is sent out of the electrophoresis box, divided images of the serum are formed in the electrophoresis box 50. Thereafter, the conveying table 21 is made to enter into the electrophoresis box 50 again, and the filter paper receiving plate 64 is lowered. At this time, a separating plate, which is provided on a filter paper pushing plate 67, descends by its own weight. Then the supporting body is separated and mounted on the belt of the conveying table.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention automatically tests serum by electrophoresis using a C-shaped support. There is Item-C related to improvement of PII equipment.

Electrophoresis has traditionally been used to test serum protein levels in hospitals. This electrophoretic test is
As is well known, serum is applied in a line on the surface of a support made of paper using a biven 1, and an electric current is applied to it to form an image of the serum to be examined. . The support that formed the image of this serum is Bonso 3R and trichloride vinegar! l! Stain with a stain solution dissolved in I solution, and further,
The portions other than the separated images are decolorized in an acetic acid solution, dried, and crushed in a clarifying liquid such as liquid paraffin to make it transparent, and then quantified by colorimetric dew 1.

Conventionally, each of these steps was performed manually using a lift, which was extremely inefficient.

Furthermore, when applying serum to a support, for example, if the applied serum does not form a constant +1j thin line, accurate measurement results cannot be obtained, so this work requires extremely skill. Furthermore, even when performed by a skilled worker, different results were often obtained depending on the worker. This happens not only in the work of applying serum on the support, but also in each step of the electrophoresis method described in (a) above.

In this way, serum testing by electrophoresis must be performed by highly trained personnel, and has the disadvantage that it is difficult to obtain accurate and uniform test results, and is inefficient because it is done manually. It also had certain drawbacks.

In order to eliminate and overcome these drawbacks of the conventional method, each step of the electrophoresis method described above is carried out automatically, and the columns are connected by an appropriate dipping device S. An automatic electrophoresis device has been developed that automatically performs all steps continuously.

However, in this automatic electrophoresis apparatus N-C, the support coated with a buffer solution is conveyed into an electrophoresis box, and then the support is sandwiched between the ends of the support through a filter paper. Electrophoresis is performed by applying electricity while holding the container. After electrophoresis, this clamping is carried out with r/? Then, it is sent to the next dyeing process. At this time, the support is loose!
Since the wet paper is coated with liquid II, it often sticks to the clamping plate and does not stick properly, which often causes malfunctions. The present invention was made in view of the above circumstances, and an object of the present invention is to make it possible to easily transport a sandwiched support within an electrophoresis box.

The electrophoresis box of the present invention will be described below based on one embodiment shown in the drawings. The explanation will include the device for transporting the support to the electrophoresis box.

From Fig. 1 to Fig. 3, J3 shows 1 a support wound into a roll, 2.3 a support feed roller, 4 a cutter,
5 determines the length of the support 7711-renly, 6 determines the rotation start time of the support feed roller 77+trenly, 10 is a wet paper web device, and buffer tank 1 containing buffer solution 12.
1. Two rollers 13 arranged so that a part of one roller 13 is immersed in the loose liquid 12, -I = I
The wet paper web roller and the guide 15 are arranged in a straight line. 1G is a feed roller made of a sponge etc. which also serves to adsorb and remove excess loose lilyte adhering to the support, and 2o is a transport roller that carries the support 1 moistened with a buffer solution into the electrophoresis box. This is a support transport device used to carry out the support after energization from inside the electrophoresis box.In this device, 21 has a cross section close to a U-shape as shown in FIG.
The kite support 22 is a transport platform with a structure long in the horizontal direction in the figure and is supported by a support 23.
It is held by a pair of bearings 24 and a pair of rollers 25, which move along the deflection guide rod 22, so as to be movable in the left-right direction in FIG. 26 is transport platform 2
A rack 27 provided on the side surface in the longitudinal direction of the gear A7 is provided to mesh with the rack 26 and is prevented from being rotated by the motor 28. The rotation of this gear A7-27 causes the rack 2G to mesh with it. Avoid moving, and avoid moving the carrier 21. 29 is a U-shaped side plate 2 of the transport platform 21.
Bells 1 to 1 are attached to the upper end surface of the frame 1a.

Further, reference numeral 30 denotes a base for determining the coating amount, and as shown in the plan view in FIG. It consists of a leg portion 32 integrally formed with this and a plurality of pairs of rollers 33 attached to the leg portion, and is disposed within the central recess of the carrier 21. Reference numeral 35 is a clamp installed on the transport stand 21, and holds the dispensing measuring stand 30 by pinching it with two leaf springs 35a. With the provided bottle, the application table 30
is placed on the carrier 21, and the I formed at the rear of it at 1ζ
This is to prevent the coating amount () table 30 from moving sideways when the conveyor table 21 is moved after being inserted into the M 30 a. 37 is used to keep the transfer bottle 38 and the slider bottle 38 from sliding into the slot 1 at all times as shown in FIG. When the coating amount ( ) 30 is placed at the J position shown in FIGS. It is designed to be inserted into.

4 and 5 show cross-sectional views of the electrophoresis box 50, and 51
is the electrophoresis box body installed on the holding table 52 with a population of 51a.
A part of it is shown in Figure 4, and as shown in C, the transport bag +i 20 has a population of 5.
It is installed so that it stands near 1a. 53 is a lid, and 54 is a window for serum application, which has a rotating body 54a with a semicircular cross-sectional shape as shown in FIG. 111 and press it onto the support to smear the serum onto the support.The blood coating tool 40 holds a number of pen nibs 4'1 on the holding plate 42. It is arranged so that it can move up and down by 1!'7. Reference numerals 43 are push rods fixed on both sides of the holding plate 42. 5G is a shutter placed at 518, and 58 is a closing operation of the shutter 56. Rod, 59 is the rod 5B.] cam to avoid force,
Reference numeral 60 denotes a motor 64 for rotating the Kamshalaf F-63 via Hebergia A7-61 and G2, and a pair of filter paper holders, each with a filter paper 65 in the buffer tank 51b formed in the electrophoresis box body 51. Accumulated #! tij1cut 6G
67 is each filter paper receiver 1) A pair of filter paper retainers placed on the stand 65; 68 is a filter paper retainer plate 67; A separation plate 69 that is slidably held downward supports a filter paper receiving plate, and a plurality of pairs of rods are provided to move this up and down, and the lower part of each rod is attached to a support plate 70. Fixed. Reference numeral 71 denotes a cam fixed to the camshaft 1-72, 73 a motor that prevents rotation of the camshaft V-shaft via gear \7-t4.75, and 76 a coating receiver ( ) A rod for moving the table 30 up and down, a nozzle fixed to the 77μ camshaft 1-72.
78 is an electrode.

Next, the operation of the electrophoresis box of the present invention will be explained. first,
The support 1 wound into a roll is fed by a support feed roller 2, and after passing through a saw blade 4, is further advanced by a support feed roller 3. When the tip of this supporting material feed reaches the flattener 5, the cutter 4 is moved based on the detection of the supporting material by the 7711-transfer sensor 5, and the supporting material is cut to a predetermined length. The coated support 1 is further advanced by the feeder 1 - roller 3 and fed into a paper pack 10 , where it is collected in a buffer solution 12 as it passes between wet paper rollers 13 and 14 and covered. The support 1 is soaked in the buffer solution by the wet paper roller 13.The tip of the support coated with the buffer solution is just stretched into the carrying bag @ 20 (XJ to the pierced bell 1 29 and the feed roller 16). When the motor 2B reaches the position where it is in contact with the
After detecting the tip of the support by the 71I-lx input 6, the L-28 is started after a predetermined time until the tip of the support reaches the bell 1 of the 2nd floor between the 29 and the roller 10. Just do it like this. Also, use motor 60 with this f A1-senri °-6! l (3 motions, electrophoresis box 50
You may also ask them to listen to the shutter 56. Thereafter, due to the rotation of the motor 28, the transport platform 21 is moved to the right side in FIG. O entry IZI 50
A is inserted inside it. When the coating tray 30 is completely inside the migration box, the motor 731 starts rotating.
As a result, the cams 71 and 77 rotate clockwise by 90 degrees as shown in Fig. 4, but these cams raise the rod 69.7G, respectively, and the filter paper holder is connected to the plate 64 (the holder is connected to the table 30 (see Fig. 5). (indicated by a chain line) is raised.As a result, the support 1 is also raised to its upper limit, but at the same time, both ends thereof are raised to +
Ai paper is held together with one end of the filter paper holder 1 and the filter paper holder 67.

In this state, a voltage is applied between both frost poles 78 to pre-energize the filter paper and the support, and the energized state is determined. When the checking is finished, the serum coating TD tool 40, which has been coated with serum on the pen tip 41 through the window 54, is lowered 1! When closed, the push rods 43 at both ends push the rotating body 54a, and the applicator 40 is inserted through the window 54 to apply the serum onto the support. Since there is a coating amount of 1.30 below this serum, the serum is well coated. When the coating is completed, the self-extinguishing applicator 40 leaves the migration box through the window 43 after one step. When the rotor 54a of this window is pushed into the recess with the symbol △ and d3 at υ, the rotor is rotated by the force Tn and the window is closed again. After that, power l\Sitoff 1-72 again
by rotating Nonom 71.77 further 90 degrees, that is, the fourth
Rotated 180 DEG from the position shown in the figure, the slide rod 69 remains in the same convexity, but the Rondoor G is lowered and the amount of coating is reduced. Next, the motor 28 is reversed to move the carrier 21 to the left in FIG.
Close the shutter 56. In this way, the electrophoresis box 50
With the area completely closed, electricity is applied for a predetermined period of time to form an image of the serum. After the electrophoresis is completed, the motor 60 is rotated to open the shutter 56 at the entrance 51a, and then the motor 73 is rotated again to move the carrier 21 to the right side in FIG. In this case, since the pins 38 of the base 1 to the collar 37 are inserted into the notches 3011 of the coating weighing table 30, only the conveying table 21 enters the migration box without moving the table 30 to measure the coating amount. Next, rotate the motor 73 to rotate the cam 71.7.
If you turn 7 further, the filter paper tray 1) plate 671 will lower.
Ru. At this time, the Annex 1 plate 6 installed on the ilJ ffd holding plate 07 was pushed up from the m m receiving () plate 64.
8 will be lowered to the position shown in the figure on your vehicle, so press the filter paper holding plate 6.
14 supports are attached to 7 and C is also attached to this separating plate 6.
8, the heald 29 of the transport platform 31 is
Get on top.

Here, C rack, gear, transport platform 31, population 51a
The support is then returned to the outside of the electrophoresis chamber and transferred to the next stage of staining, decolorization, etc.

In addition, an outlet is installed on the side opposite to the inlet of the electrophoresis box, and the support is transferred to the next step after the above-mentioned electrophoresis 1FII is completed.
The +1f1 machine 21 may be taken out from this exit and moved to the next step by appropriate means. In addition, a 114-built 114-built 11ρ stand similar to this 1ffJ stand 2'1 was placed outside one of these outlets, and this was placed into the electrophoresis box from the exit side to carry away the support after electrophoresis. It's so hot. In this way, a platform that also has an exit in the migration box is
Needless to say, it would be better to install a 1j4-built Shitotsuta machine 1111 similar to the one at the entrance.

As explained above, in the electrophoresis apparatus C of the present invention, the support is held between the receiver and the presser, and when the support is stagnated, the support separating means provided on the presser is used. Since the IIT (indeed R1) is increased by the IIT (R1), the conveyance of the support is smooth (J).

[Brief explanation of the drawing]

Figure 1 is a side view of the conveyance device that conveys the support, Figure 2 is a plan view of the conveyor table and the coating measuring table, and Figure 3 is the conveyor table and the coating plate 5.
FIG. 4 is a cross-sectional view of the electrophoresis box of the present invention, and FIG. 5 is a cross-sectional view of the receiver 4-1. 50... Electrophoresis box, 51... Electrophoresis box wooden body, 5
3...Lid, 54...Window, 56...Shutter, 0
4... Filter paper receiver JJ board, 67... Filter paper presser plate. m-"l" Figure 3 Figure 4 0 626I ゝ60 Figure 5

Claims (1)

[Scope of Claims] In an electrophoresis device in which a sample is electrophoresed on a support by applying a voltage to both ends of the support coated with a sample such as serum, the support A support valve that is held between a presser plate that presses from the upper surface of the end and a support that presses from the lower surface of the end, and that is slidable up and down on the presser plate when the clamping is released. 1'll
Particularly, the presser plate is separated by one means.
A neat electrophoresis device.