JPS57197034A - Preparation of immobilized nucleic acid - Google Patents
Preparation of immobilized nucleic acidInfo
- Publication number
- JPS57197034A JPS57197034A JP56081709A JP8170981A JPS57197034A JP S57197034 A JPS57197034 A JP S57197034A JP 56081709 A JP56081709 A JP 56081709A JP 8170981 A JP8170981 A JP 8170981A JP S57197034 A JPS57197034 A JP S57197034A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- insolubilize
- preparation
- irradiated
- immobilized nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
PURPOSE: To immobilize and insolubilize nucleic acid, by a method wherein nucleic acid and a water-soluble high-molecular base material are mixed and irradiated with glow discharge plasma to insolubilize the surface layer part thereof by crosslinked gel formation.
CONSTITUTION: Nucleic acid is mixed with a water-soluble high molecular material as a matrix such as polyvinyl alcohol or gelatine and the mixture is irradiated with glow discharge plasma to insolubilize the surface layer part thereof by crosslinked gel formation. By utilizing this process, nucleic acid is confined into the matrix to prepare immobilized nucleic acid which is useful as a filler material of affinity chromatography and an adsorbent material in an antigen-antibody reaction.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56081709A JPS57197034A (en) | 1981-05-28 | 1981-05-28 | Preparation of immobilized nucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56081709A JPS57197034A (en) | 1981-05-28 | 1981-05-28 | Preparation of immobilized nucleic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57197034A true JPS57197034A (en) | 1982-12-03 |
JPS6128375B2 JPS6128375B2 (en) | 1986-06-30 |
Family
ID=13753911
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56081709A Granted JPS57197034A (en) | 1981-05-28 | 1981-05-28 | Preparation of immobilized nucleic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57197034A (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001033227A1 (en) * | 1999-11-04 | 2001-05-10 | Center For Advanced Science And Technology Incubation, Ltd. | Method for immobilizing material |
WO2004046714A1 (en) * | 2002-11-19 | 2004-06-03 | Daicel Chemical Industries, Ltd. | Separating agent for optical isomer |
WO2004091753A1 (en) * | 2003-04-16 | 2004-10-28 | Nissei Bio Co., Ltd. | Method of removing harmful substances and filter for removing harmful substances |
JP2006223241A (en) * | 2005-02-21 | 2006-08-31 | National Printing Bureau | Dna-containing material |
WO2007008755A2 (en) * | 2005-07-08 | 2007-01-18 | The Board Of Regents, The University Of Texas System | Surface functionalization of polymeric materials |
JP2007277447A (en) * | 2006-04-10 | 2007-10-25 | Inoac Corp | Nucleotide chain carrier and application thereof |
JP2008001763A (en) * | 2006-06-21 | 2008-01-10 | Gunma Univ | Method for producing gel composition stably containing deoxyribonucleic acid and the resultant gel composition |
JP2008030039A (en) * | 2007-08-13 | 2008-02-14 | Nissei Bio Kk | Method for removing harmful substance and filter for removing harmful substance |
JP2008068254A (en) * | 2007-08-13 | 2008-03-27 | Nissei Bio Kk | Method and filter for removing harmful substance |
JP2008272657A (en) * | 2007-04-27 | 2008-11-13 | Canon Inc | Dna fixing method, dna porous composite and purification system using this composite |
CN106854619A (en) * | 2017-01-19 | 2017-06-16 | 西安交通大学 | A kind of crosslinking apparatus based on plasma, application method and application |
-
1981
- 1981-05-28 JP JP56081709A patent/JPS57197034A/en active Granted
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001033227A1 (en) * | 1999-11-04 | 2001-05-10 | Center For Advanced Science And Technology Incubation, Ltd. | Method for immobilizing material |
US7407576B2 (en) | 2002-11-19 | 2008-08-05 | Daicel Chemical Industries, Ltd. | Separating agent for an optical enantiomeric isomer |
WO2004046714A1 (en) * | 2002-11-19 | 2004-06-03 | Daicel Chemical Industries, Ltd. | Separating agent for optical isomer |
US8092677B2 (en) | 2002-11-19 | 2012-01-10 | Daicel Chemical Industries, Ltd. | Separating agent for an enantiomeric isomer |
WO2004091753A1 (en) * | 2003-04-16 | 2004-10-28 | Nissei Bio Co., Ltd. | Method of removing harmful substances and filter for removing harmful substances |
JP2006223241A (en) * | 2005-02-21 | 2006-08-31 | National Printing Bureau | Dna-containing material |
WO2007008755A2 (en) * | 2005-07-08 | 2007-01-18 | The Board Of Regents, The University Of Texas System | Surface functionalization of polymeric materials |
WO2007008755A3 (en) * | 2005-07-08 | 2007-04-19 | Univ Texas | Surface functionalization of polymeric materials |
JP2007277447A (en) * | 2006-04-10 | 2007-10-25 | Inoac Corp | Nucleotide chain carrier and application thereof |
JP2008001763A (en) * | 2006-06-21 | 2008-01-10 | Gunma Univ | Method for producing gel composition stably containing deoxyribonucleic acid and the resultant gel composition |
JP2008272657A (en) * | 2007-04-27 | 2008-11-13 | Canon Inc | Dna fixing method, dna porous composite and purification system using this composite |
JP2008068254A (en) * | 2007-08-13 | 2008-03-27 | Nissei Bio Kk | Method and filter for removing harmful substance |
JP2008030039A (en) * | 2007-08-13 | 2008-02-14 | Nissei Bio Kk | Method for removing harmful substance and filter for removing harmful substance |
CN106854619A (en) * | 2017-01-19 | 2017-06-16 | 西安交通大学 | A kind of crosslinking apparatus based on plasma, application method and application |
CN106854619B (en) * | 2017-01-19 | 2023-10-20 | 西安交通大学 | Crosslinking device based on plasma, using method and application |
Also Published As
Publication number | Publication date |
---|---|
JPS6128375B2 (en) | 1986-06-30 |
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