JPH0338511A - External preparation - Google Patents

Abstract

PURPOSE:To obtain an external preparation improving physiological function of the skin, providing the skin with tension, having excellently beautifying effects on the skin, capable of supplying the hair with smoothness, luster and finishing effects, containing transglutaminase. CONSTITUTION:An external preparation containing 0.001-20wt.%, especially 0.01-5wt.% based on the whole amount of external preparation of transglutaminase. In application of transglutaminase to external preparation, in order to prevent reduction in activity, the external preparation is prepared preferably by blending with vaseline, by two-pack type of dissolution and preparation with powdor base, water or toilet lotion, etc., in use or by stable blending with aqueous base through microcapsule.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention improves physiological functions for the skin, gives firmness to the skin, and has excellent skin beautifying effects.
The present invention relates to a novel external preparation that can impart smoothness, shine, and finishing effects to hair.

(Prior art) Conventionally, due to various factors such as aging and dryness, the skin has suffered from decreased physiological functions, decreased firmness, skin irritation, etc., and hair has also suffered from various problems such as decreased smoothness and luster. . To improve these symptoms, external preparations, especially for the skin, include various moisturizers such as glycerin, 1,3-
Butylene glycol, hyaluronic acid, etc. have been used.

Various silicone oils and various oils are also used for hair to improve its smoothness and shine.

(Problems to be Solved by the Invention) However, the above-mentioned substances that have been used in the past only have the effect of supplying moisture to the stratum corneum of the skin, and they also have a negative effect on the hair due to temperature, humidity, wind, etc. When environmental conditions are poor due to the influence of sunlight, it is often not as effective as expected.

(Means for Solving the Problems) As a result of intensive research to overcome the above problems, the present inventors found that when transglutaminase, which is a type of in-vivo enzyme, is applied to the skin or hair, the skin It has been discovered that physiological functions and skin beautification effects can be enhanced, and the smoothness and shine of hair can be significantly improved, and based on this knowledge, the present invention has been completed.

That is, the present invention relates to an agent for external use characterized by containing transglutaminase.

The present invention will be explained in detail below.

First, the transglutaminase applied to the present invention is
Molecular weight 30,000 to 1 distributed in the organs of animals including humans
It is an enzyme protein of about 1,000,000, and is known to have the effect of specifically cross-linking lysine residues and glutamine residues in the skin with ε (γ-glutamyl-lysine) bonds in vivo. .

In addition, although such transglutaminase exists as a standard substance for measuring enzyme activity and as a reagent, there have been no examples of using it externally, and furthermore, in that case, there is no effect. It was also completely unknown.

In order to actually obtain this, it is generally extracted from the liver of a guinea pig or the like using conventional methods such as physical or biochemical means, fractionated and purified, and freeze-dried to form a powder. .

However, since the activity of transglutaminase decreases over time in an aqueous solution and instantaneously in an organic solvent, when applying it to the external preparation of the present invention, in order to prevent a decrease in activity, transglutaminase should be mixed with vaseline or powdered. Two-dose formulation prepared by dissolving the base and water or lotion before use, or stabilizing formulation into a water-soluble base using microcapsules (Japanese Patent Publication No. 55-1
No. 41405> etc., and it is also preferable to prepare it at room temperature.Next, the transglutaminase content in the external preparation should be 0.001 to 20% by weight based on the total amount of the external preparation. Preferably, especially o, o
i to 5% by weight is more preferred.

Here, if the amount is less than 0.001% by weight, it is difficult to expect the desired effect, and on the other hand, if the amount exceeds 20% by weight, no enhancement of the effect commensurate with the increase in amount is observed, which is uneconomical.

In general, the external preparation of the present invention may contain oils such as hydrocarbons, triglycerides, and higher alcohols, humectants such as glycerin, preservatives such as parabens, etc., as necessary, to the extent that the effects of the present invention are not impaired. Nonionic, anionic, and cationic surfactants, fragrances, etc. can be used, and these ingredients can be used as compositions for solubilizing systems such as lotions, emulsifying systems such as creams and emulsions, cartilage, powders, etc. You can take a mold.

(Example) Next, the present invention will be explained with reference to Examples, but the present invention is not limited thereto. The blending ratios are in parts by weight.

In addition, an evaluation test was conducted using both Examples and Comparative Examples.

[See the blank space below. Table 1. Essence (Method) The ingredients shown in Table 1 above are sealed in a polyethylene bag and mixed until uniform to obtain a powdered essence.

This is divided into 0.5 to 2 g packages, and when used, approximately 5 to 1
Disperse in 0x amount of water or lotion and apply to face or body.

(Evaluation test) The essence of Example 1 was applied to the entire back of the right hand and the essence of Comparative Example 1 was applied to the back of the left hand three times a day using the above method for 30 women with dry or rough skin. ,5
A continuous use test was conducted for 0 days. The implementation period was from January to February, when dryness tends to occur, and judgments were made on the 25th and 50th day.
I did it on the first day. The evaluation method was as follows: Participants were asked to report how their right hand had improved compared to their left hand using the following criteria. The results are shown in Table 2.

As shown in Table 2, it can be seen that the products of the present invention are excellent in improving dry skin and rough skin, and have a high skin beautifying effect.

milky lotion No. table （＊）： Known nylon microencapsulation method More nylon microcapsules Transglutaminase.

(Method) Components 1 to 10 shown in Table 3 above are mixed in a conventional manner to obtain a milky lotion.

(Evaluation test) 28 adult female panelists were arbitrarily divided into 2 groups of 14 each, one group received the Milky Lotion of Example 2, and the other group received the Milky Lotion of Comparative Example 2. A 30-day continuous application test was conducted by having all types apply the product twice a day after washing in the morning and before going to bed at night.

The evaluation was based on the results of the sensory evaluation before and after the continuous use test.
Shown in the table.

teeth, skin firmness, Excellent for improving moist feeling I understand that.

3. Shampoo (Method) Components 1 to 6 shown in Table 5 above are sealed in a polyethylene bag and mixed until uniform to obtain a powdered shampoo.

Take an appropriate amount of this, and when using it, redistribute it in about 5 times the amount of water, apply it all over your hair, and rinse it off.

(Evaluation test) Example 3 and Comparative Example 3 on 22 adult female panelists
We conducted actual hair washing tests on both shampoos.
The smoothness, shine, and combability of the hair were evaluated.

The results are shown in Table 6.

Table 6 As shown in Table 6, it can be seen that the products of the present invention have superior effects on hair smoothness, shine, and combability compared to comparative amounts.

(Effects of the Invention) As detailed above, the present invention, by containing transglutaminase, gives firmness to the skin, improves the physiological functions of the skin, has excellent skin beautifying effects, and has smoothness and smoothness for hair. It can add luster and finish to the hair.

Claims (1)

[Claims] 1) An external preparation characterized by containing transglutaminase.