JP7357400B1 - Anti-aging composition, manufacturing method and application - Google Patents

Abstract

An object of the present invention is to provide an anti-aging composition, a manufacturing method, and an application. The present invention discloses an anti-aging composition, a manufacturing method, and its application, and includes nanoliposomes, diglucosyl gallic acid, glucosyl hesperidin, Laminaria japonica extract, biotin, and nanoliposomes loaded into the nanoliposomes. and an active ingredient consisting of caffeine. The present invention further discloses the application of the anti-aging composition to the production of cosmetics. The anti-aging composition of the present invention can promote the proliferation of human skin fibroblasts and the production of collagen, and can improve the transdermal absorption performance of the anti-aging composition, thereby improving wrinkles. Skin tone is improved, damaged skin is repaired, and dry skin is improved. [Selection diagram] Figure 1

Description

The present invention belongs to the field of cosmetics and relates to anti-aging compositions, in particular anti-aging compositions, manufacturing methods and applications.

The epidermal tissues and cells of human skin not only protect from external invasion through the epidermal barrier effect, but also have the effect of slowing down the aging process of the skin tissue through water retention or moisturizing mechanisms that prevent water evaporation in the skin tissue. able to demonstrate. The barrier construction and moisturizing/antiaging effects of the epidermal tissue of the skin are extremely important and essential for the survival of organisms and tissue protection. Skin tissue cells, like other cells in the human body, undergo aging and decline in structure, composition, function, etc. with the passage of time and the aging of the organism, and produce many metabolic waste products, inactivation and aging. The inability to effectively remove damaged cells and subcellular structures, resulting in an imbalance between normal and steady-state physiological cells, accelerates skin aging and increases the susceptibility to disease.

Aging is a process in which, after an organism passes through a period of sexual maturity, the self-renewal and repair capacity of cells decreases, the structure and function of tissue organs degenerates, and ultimately leads to death, and the pressure stress capacity decreases. , characterized by an imbalance and an increased risk of contracting the disease. With the aging of the global population, various geriatric degenerative diseases and the huge medical costs associated with them are becoming increasingly serious social issues. Maintaining the health status of the elderly is an important link in reducing the social and economic burden associated with population aging. Therefore, it is of great significance to consider measures and methods to affect aging and aging-related diseases that are simple, easy to implement, economical, effective, safe and easy to develop.

Until now, the use of anti-aging drugs is a common anti-aging measure. Many of the drugs used clinically to delay aging include vitamin E, which can promote cell division and suppress the production of oxyradicals, procaine preparations, which can extend the lifespan of cells, and drugs that can delay brain aging. Piracetam is a synthetic aspirin-like drug that reduces the decline in the body's functions due to aging by resisting oxidative stress.

Skin aging is a skin aging phenomenon caused by natural or non-natural factors. After a person is born, the skin tissue grows day by day, its functions gradually become more active, and at a certain age it begins to degenerate, and the aging of the skin progresses without realizing it. The period of growth of skin tissue, called the "turning point of the skin," generally ends around the age of 25. After this, growth progresses with aging, and the elastic fibers of the skin become coarser, leading to the onset of old age.40- Skin aging becomes apparent at the age of 50, but the degree of aging differs from person to person. With skin aging, the skin becomes thinner and atrophic, subcutaneous fat decreases, and changes in elastic fibers and collagen fibers cause the skin to gradually lose its elasticity and tension, further causing sagging and wrinkles.

Currently, in the field of medical beauty and anti-aging, in addition to facelift surgery and small plastic surgery using implanted thread lifts, there are treatments such as filling the skin with collagen, elastin, and hyaluronic acid, photoelectric treatments such as Thermage, syringe injections, etc. Similar to cosmetic surgery treatments, fibroblasts are activated by stimulating cells with light and high-frequency radio waves to promote collagen production. should stimulate fibroblast behavior after undergoing a mild burn injury. Recovery of burn areas mainly depends on fibroblasts, and fibroblast regenerative medicine injects cultured fibroblasts directly into the dermal layer to directly replenish regenerated cells, increase cell number, and stimulate cell division. It increases the speed and continues to produce substances such as collagen in the skin to return to a youthful skin state, and the effect is long-lasting, natural, and can be continuously cultivated and used.

As the number of fibroblasts decreases with increasing age, such anti-aging medical methods require multiple injections or facelifts or thread implants on the skin surface.

Currently, anti-aging cosmetics or wrinkle-improving cosmetics for the skin surface have appeared on the market, but in practical use, the active ingredients do not penetrate the skin to exert their biological effects and are difficult to absorb. There are deficiencies and problems such as the wrinkle improvement effect is slowly achieved and the effect is not significant.

Therefore, there is a need for compositions or products containing such compositions that can promote dermal fibroblast proliferation and collagen production.

In a first aspect of the present invention, in order to solve the above problems, the present invention can promote the proliferation of human skin fibroblasts and the production of collagen, and improve the transdermal absorption performance of an anti-aging composition. To provide an anti-aging composition that improves wrinkles, improves skin color, repairs damaged skin, and improves dry skin.

In a second aspect, the present invention further provides a method for producing the above anti-aging composition.

In a third aspect, the present invention further provides an application of the above anti-aging composition.

In order to achieve the above object, the present invention adopts the following technical solutions.

In a first aspect, the present invention provides an anti-aging drug comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the active ingredient including diglucosyl gallic acid, glucosyl hesperidin, Lacustilla chinensis extract, biotin, and caffeine. A composition is provided.

In the present invention, the microbial layer of the skin is capable of producing α-glucosidase, and diglucosyl gallic acid cleaves the glucose bond of diglucosyl gallic acid to produce trihydroxybenzoic acid through the action of α-glucosidase. By quickly acting on the target, melanin pigment production is reduced, and trihydroxybenzoic acid is highly effective in reducing free radicals and inhibiting tyrosinase activity. It is possible to suppress the expression of the enzyme that produces melanin pigment and to suppress the movement of melanin pigment to the surface layer of the skin, and by suppressing the movement of melanin pigment, effects such as reducing melanin pigment can be achieved. Diglucosyl gallic acid can effectively suppress the activity of tyrosinase and prevent the movement of melanin pigment, thereby clearly erasing age spots, brightening the skin color, and increasing the whiteness of the skin. The acid furthermore shows a high anti-free radical effect and has an anti-aging effect.

Glucosyl hesperidin is a natural bioflavonoid extracted from citrus peel and is a water-soluble derivative of hesperidin. By improving blood circulation and promoting circulation on the skin surface, it can improve dark circles, and the flavonoid compounds in glucosyl hesperidin have the effects of antioxidant, ultraviolet absorption and free radical scavenging, and also improve collagen structure. can be effectively strengthened.

Laminaria extract promotes the production of collagen and laminin in the skin, reduces facial muscle contractions, reduces and/or prevents fine lines or wrinkles on the face, and improves the dynamic appearance of facial redundant muscle contractions. reducing facial and forehead wrinkles (e.g., facial wrinkles), increasing collagen production, reducing matrix metalloproteinases, firming skin, and/or loose, drooping, and lax skin conditions. can be used to improve

Biotin is also called vitamin H or coenzyme R, and is a water-soluble vitamin that also falls under vitamin B group B7. It is required for the synthesis of vitamin C, which is essential for the normal metabolism of fats and proteins. It is also an essential nutrient for the natural growth, development and maintenance of normal functional health of the human body. Biotin has excellent beauty effects and can maintain clear, white, and fine-textured skin.

Caffeine is a white powder with a characteristic odor and has the effect of promoting cellular metabolism and accelerating local microcirculation.

The active ingredients, which are blended with caffeine, biotin, Laminaria japonica extract, glucosyl hesperidin, and diglucosyl gallic acid, are effectively absorbed into the skin and effectively reduce melanin pigment deposition, making it free from external invasion. It can resist radicals and effectively suppress the activity of inflammatory factors, reduce skin water loss, thereby effectively improving and erasing dark circles, delaying skin aging, and improving skin Increase gloss.

Furthermore, loading active ingredients into nanoliposomes is highly stable, gentle, and less irritating to the skin, avoiding structural destruction of active ingredients during the manufacturing process.

As a preferred solution of the invention, the anti-aging composition comprises, in weight percentages, 0.011%-1.1% diglucosyl gallic acid, 0.01%-1.5% glucosyl hesperidin, 0. 0.01% - 0.8% Laminaria extract, 0.00001% - 0.0005% Biotin, 0.00001% - 0.0005% Caffeine, 0.6% - 2.6% Cholesterol , 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water.

As a preferred solution of the present invention, the anti-aging composition comprises, in weight percentages, 0.011%-0.5% diglucosyl gallic acid, 0.01%-0.5% glucosyl hesperidin, 0. 0447% - 0.6% Laminaria japonica extract, 0.00001% - 0.0005% biotin, 0.00001% - 0.0005% caffeine, 0.6% - 2.6% cholesterol , 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water.

As a preferred solution of the present invention, the anti-aging composition comprises, in weight percent, 0.011% diglucosyl gallic acid, 0.5% glucosyl hesperidin, 0.0447% Lacustnia chinensis extract, 0. .0001% biotin, 0.0001% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the remainder deionized water.

As a preferred solution of the present invention, the particle size of the nanoliposomes is 100-250 nm.

In a second aspect of the present invention,
a) dissolving the active ingredient and nanoliposome to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. After completing the treatment, the method for producing the anti-aging composition is provided, which includes the step of filtering to obtain the anti-aging composition.

In a third aspect, the present invention provides an application of the above-mentioned anti-aging composition to the production of cosmetics, and the cosmetics include lotion, face pack, essence, eye cream, eye pack, skin care gel, skin care emulsion, face Contains cream or spray.

As a preferred solution of the present invention, the cosmetic product contains at least one of a solvent, a thickener, a preservative, a pH adjuster, a humectant, an emollient, a chelating agent, an emulsifier, an essential oil, a fragrance, and a pigment. Further included are additives that include.

As a preferred solution of the present invention, the cosmetic product comprises, in parts by weight, 0.01-60 parts of the anti-aging composition, 0.1-0.8 parts of hydrogenated rapeseed oil alcohol, 5-diisostearyl malate. 10 parts, 1,3-propanediol 5-10 parts, coconut oil alkyl caprylate 3-12 parts, macadamia nut fatty acid phytosteryl 0.01-0.1 part, retinol palmitate 0.01-2 parts, hesperidin methyl chalcone 0 0.01-0.5 parts and 50-80 parts deionized water.

As a preferred solution of the present invention, the method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, and make an eye cream. and a step of obtaining.

Compared with the prior art, the present invention has the following beneficial effects.

1) The present invention is highly stable and gentle by blending caffeine, biotin, Laminaria chinensis extract, glucosyl hesperidin, and diglucosyl gallic acid as active ingredients, and loading the active ingredients with nanoliposomes. , has low irritation to the skin, avoids structural destruction of active ingredients during the manufacturing process, is more effectively absorbed by the skin, effectively reduces melanin pigmentation, resists external invasion and free radicals, It can also effectively suppress the activity of inflammatory factors and reduce skin water loss, thereby effectively improving and erasing dark circles, delaying skin aging, and increasing skin gloss.

2) The anti-aging composition of the present invention can promote the proliferation of skin fibroblasts and the production of collagen, thereby improving wrinkles, improving skin tone, and repairing damaged skin. , dry skin is improved.

FIG. 3 is a diagram showing the results of promoting cell proliferation according to the present invention. FIG. 3 is a diagram showing the results of collagen production according to the present invention.

In addition, numerous specific details are set forth in the following detailed description to better explain the invention. As will be understood by those skilled in the art, the invention may equally be practiced without the specific details. In other instances, methods, means, equipment and steps that are familiar to those skilled in the art have not been described in detail in order to obscure the spirit of the invention.

It should be explained that in this specification, the use of "capable" or "possible" means both performing a certain process and not performing a certain process. In this specification, the value range indicated by "value A-value B" refers to a range that includes endpoint values A and B.

Unless otherwise specified, all units used in the present invention are international units, and it should be understood that all values and ranges of values appearing in the present invention include tolerances for industrial production.

All of the raw materials used in the present invention, such as diglucosyl gallic acid, glucosyl hesperidin, Laburnum orandascenchi extract, biotin, and caffeine, are purchased from the market.

The Acmella oleracea extract used is a water-alcoholic extract, the solvent comprises a liquid mixture of water, ethanol and 1,3-propanediol, and the ethanol is removed from the resulting extract. be done.

In the present invention, additives used in cosmetics are commonly used in the conventional cosmetics market, such as solvents, thickeners, preservatives, pH regulators, humectants, emollients, chelating agents, emulsifiers, essential oils, fragrances, and pigments. It may also be an additive used.

Example 1
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percentages, 0.011% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 5% glucosyl hesperidin, 0.0447% Lacustilla chinensis extract, 0.0001% biotin, 0.0001% caffeine, 1.8% cholesterol, 1.2% behenyl alcohol, 6.3% of hydrogenated lecithin, and the balance of deionized water.

The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the anhydrous ethanol in the mixed solution of step a), add deionized water at 40 ° C., perform ultrasound treatment at 15 Hz ultrasound frequency for 15 minutes, and after completing the ultrasound treatment, and filtering to obtain an anti-aging composition.

Example 2
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 1.1% diglucosyl gallic acid; 1.1% diglucosyl gallic acid; 5% glucosyl hesperidin, 0.8% Lavender chinensis extract, 0.0005% biotin, 0.0005% caffeine, 2.6% cholesterol, 1.5% behenyl alcohol, 6.5% of hydrogenated lecithin, and the balance of deionized water.

The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the anhydrous ethanol in the mixed solution of step a), add deionized water at 50 °C, and perform ultrasonic treatment at 25 Hz ultrasonic frequency for 18 minutes, after completing the ultrasonic treatment, and filtering to obtain an anti-aging composition.

Example 3
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percentages, 0.011% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% diglucosyl gallic acid, 0.01% glucosyl hesperidin, 0.01% Lacustilla chinensis extract, 0.00001% biotin, 0.00001% caffeine, 0.6% cholesterol, 0.1% behenyl alcohol, 6% water Contains added lecithin and the balance deionized water.

The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Evaporate and remove the absolute ethanol in the mixed solution of step a), add deionized water at 60°C, and perform ultrasonic treatment at an ultrasonic frequency of 15-35 Hz for 15-20 minutes. and filtering to obtain an anti-aging composition.

Example 4
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 0.011% diglucosyl gallic acid, 1. 2% glucosyl hesperidin, 0.6% Lavender chinensis extract, 0.0002% biotin, 0.000078% caffeine, 1.8% cholesterol, 0.9% behenyl alcohol, 6.2% of hydrogenated lecithin, and the balance of deionized water.

The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 45 °C, perform ultrasonic treatment at 15 Hz ultrasonic frequency for 20 minutes, and after completing the ultrasonic treatment, filter. and obtaining an anti-aging composition.

Example 5
This example provides an anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, the anti-aging composition comprising, in weight percent, 0.43% diglucosyl gallic acid, 1. 12% glucosyl hesperidin, 0.0447% Lacustilla dianthus extract, 0.0001% biotin, 0.0004% caffeine, 0.9% cholesterol, 1.1% behenyl alcohol, 6% water Contains added lecithin and the balance deionized water.

The method for producing the anti-aging composition is as follows:
a) dissolving diglucosyl gallic acid, glucosyl hesperidin, Laburnum chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in absolute ethanol at a mixing ratio to obtain a mixed solution;
b) Remove the anhydrous ethanol in the mixed solution of step a) by evaporation, add deionized water at 55 ° C, and perform ultrasonic treatment at 35 Hz ultrasonic frequency for 15 minutes, after completing the ultrasonic treatment. and filtering to obtain an anti-aging composition.

Example 6
This example shows, in parts by weight, 12.5 parts of the anti-aging composition obtained in Example 1, 0.5 parts of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, and 1,3-propanediol. 10 parts of coconut oil alkyl caprylate, 0.05 part of macadamia nut fatty acid phytosteryl, 0.5 part of retinol palmitate, 0.2 part of hesperidin methyl chalcone, and 80 parts of deionized water. do.

The method for producing the eye cream for wrinkle improvement includes:
1) Hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, PHYTOSTERYL MACADAMIATE, purchased from Aoke New Material Technology (Shanghai) Co., Ltd. is a compound that can be purchased from multiple suppliers (International Cosmetic Ingredient Dictionary). - Handbook, 12th Edition, Volume 1, Page 1146 (2008), the contents of which are incorporated herein by reference).On the other hand, the ingredient is Sederma SAS (France), sold under the trade name EyelissTM. can be obtained by using a mixture, which is a combination of water, glycerol, hesperidin methyl chalcone, steareth-20, dipeptide-2 (valine-tryptophan), and palmitoyltetrapeptide-7 (Pal-GQPR). Adding and stirring uniformly, heating to 50 ° C. and stirring uniformly to form an oil phase,
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction vessel of a supercritical apparatus, and bring the temperature inside the reaction vessel of the supercritical apparatus to 55. ℃, set the pressure to 12Mpa and time to 30 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .

Example 7
This example includes, in parts by weight, 25 parts of the anti-aging composition obtained in Example 1, 0.8 parts of hydrogenated rapeseed oil alcohol, 10 parts of diisostearyl malate, 5 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, which contains 3 parts of coconut oil alkyl caprylate, 0.01 part of macadamia nut fatty acid phytosteryl, 0.01 part of retinol palmitate, 0.01 part of hesperidin methyl chalcone, and 50 parts of deionized water.

The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir uniformly, heat to 90°C, and stir uniformly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction vessel of a supercritical device, and bring the temperature inside the supercritical device reaction vessel to 65%. ℃, set the pressure to 14 MPa and time to 30 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain eye cream. .

Example 8
This example includes, in parts by weight, 50 parts of the anti-aging composition obtained in Example 1, 0.1 part of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, 7 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, comprising 12 parts of coconut oil alkyl caprylate, 0.1 part of macadamia nut fatty acid phytosteryl, 2 parts of retinol palmitate, 0.5 part of hesperidin methyl chalcone, and 75 parts of deionized water.

The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir evenly, heat to 80°C, and stir evenly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water and 1,3-propanediol to form an aqueous phase;
3) The oil phase obtained in step 1) and the aqueous phase obtained in step 2) are mixed, and the resulting mixture is placed in the reaction vessel of a supercritical device until the temperature inside the supercritical device reaction vessel reaches 45%. ℃, set the pressure to 15 MPa and time to 40 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .

Example 9
This example includes, in parts by weight, 100 parts of the anti-aging composition obtained in Example 1, 0.1 part of hydrogenated rapeseed oil alcohol, 5 parts of diisostearyl malate, 7 parts of 1,3-propanediol, To provide an eye cream for improving wrinkles, comprising 12 parts of coconut oil alkyl caprylate, 0.1 part of macadamia nut fatty acid phytosteryl, 2 parts of retinol palmitate, 0.5 part of hesperidin methyl chalcone, and 75 parts of deionized water.

The method for producing the eye cream for wrinkle improvement includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, alkyl coconut oil caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methyl chalcone, stir evenly, heat to 80°C, and stir evenly. , a step of forming an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water and 1,3-propanediol to form an aqueous phase;
3) The oil phase obtained in step 1) and the aqueous phase obtained in step 2) are mixed, and the resulting mixture is placed in the reaction vessel of a supercritical device until the temperature inside the supercritical device reaction vessel reaches 45%. ℃, set the pressure to 15 MPa and time to 40 minutes, reduce the pressure to release carbon dioxide, and cool to room temperature to obtain an eye cream. .

The anti-aging composition obtained in Example 1 is subjected to a cell proliferation promoting effect test and a collagen production effect test.

In the control group, no anti-aging composition is added.

Normal human skin fibroblasts are used as cells.

The cell proliferation promoting effect test method is as follows.

1) The grown cells are dispersed in Dulbecco's MEM medium containing FBS and inoculated into a 96-well culture plate. Subsequently, the cells are cultured in a CO ₂ incubator (at 37° C.).

2) On the second day after inoculation, the medium was prepared with FBS supplemented with the anti-aging composition obtained in Example 1 (adding 12.5%, 25%, 50%, and 100% by mass percentage, respectively). Change to Dulbecco's MEM medium containing and Dulbecco's MEM medium containing FBS of the control group, followed by culturing in a CO ₂ incubator.

3) On the last day of culture, remove the medium in the plate, prepare MTT solution with Dulbecco's MEM containing FBS, and culture in a _CO2 incubator. Thereafter, the extract and microparticles are dissolved, and the absorbance is measured using a speed counter, which is used as a value to indicate the number of cells. The measurement results were used as a control and converted to 100, and if necessary, a significant difference test was performed using Student's t test, and the results are shown in FIG. 1.

In combination with FIG. 1, the contents of 25% and 50% have a clear promoting effect on the increase of human skin fibroblasts. However, there is clearly no significance at 12.5% and 100% contents.

The collagen production promotion test method is as follows.

1) The grown cells are dispersed in Dulbecco's MEM medium containing FBS and inoculated into a 96-well culture plate. Subsequently, the cells are cultured in a CO ₂ incubator (at 37° C.).

2) On the second day after inoculation, the culture medium was prepared using Dulbecco's anti-aging composition obtained in Example 1 (added at 12.5%, 25%, and 50% by mass percentage, respectively) and FBS as a control group. The medium is replaced with a medium supplemented with MEM medium, followed by culturing in a CO ₂ incubator.

3) Then, collect the supernatant and measure the amount of type I collagen by ELISA method. The amount of type I collagen protein per unit in the supernatant was collected, calculated and estimated using lysed cells, and evaluated. The results are shown in FIG. 2.

Combined with Figure 2, when the eye cream containing the anti-aging composition was added to the medium, the amount of type I collagen per unit of protein increased compared to the control group, and when 25% was added, A significant difference is observed.

From the above, the anti-aging composition of the present invention has a proliferation-promoting effect on normal human skin fibroblasts and a collagen production-promoting effect, and type I collagen is clearly related to skin elasticity and strength. Therefore, when the anti-aging composition of the present invention is added to a product, it has the effect of improving skin elasticity and reducing wrinkles.

The above description is only a preferred embodiment of the invention and is not intended to substantially limit the invention in any way. It is noted that those skilled in the art can make various further improvements and supplements without departing from the method described in the present invention, and these improvements and supplements should be considered as the protection scope of the present invention. Any number of changes, modifications and evolutions that can be made by those skilled in the art using the technical content disclosed above without departing from the spirit and scope of the present invention are equivalents of the present invention. Examples, and any equivalent changes, modifications and evolutions made to the above embodiments based on the substantive technology of the present invention shall still be within the scope of the technical solutions of the present invention. .

[Additional notes]
[Additional note 1]
1. An anti-aging composition comprising a nanoliposome and an active ingredient loaded into the nanoliposome, including diglucosyl gallic acid, glucosyl hesperidin, Lacustilla japonica extract, biotin, and caffeine.

[Additional note 2]
In weight percentages, 0.011%-1.1% diglucosyl gallic acid, 0.01%-1.5% glucosyl hesperidin, 0.01%-0.8% Lacustrum extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 Anti-aging composition according to appendix 1, characterized in that it comprises .5% hydrogenated lecithin and the balance deionized water.

[Additional note 3]
In weight percentages, 0.011%-0.5% diglucosyl gallic acid, 0.01%-0.5% glucosyl hesperidin, 0.0447%-0.6% Laminaria japonica extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 Anti-aging composition according to appendix 1, characterized in that it comprises .5% hydrogenated lecithin and the balance deionized water.

[Additional note 4]
In weight percentages, 0.011% diglucosyl gallic acid, 0.5% glucosyl hesperidin, 0.0447% Lacustnia chinensis extract, 0.0001% biotin, 0.0001% caffeine, 0.6 %-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water. Anti-aging composition as described.

[Additional note 5]
The anti-aging composition according to any one of appendices 1 to 4, wherein the nanoliposome has a particle size of 100 to 250 nm.

[Additional note 6]
a) dissolving the active ingredient and nanoliposome to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. After completing the treatment, the method for producing an anti-aging composition according to any one of Supplementary Notes 1 to 5, comprising the step of filtering to obtain an anti-aging composition.

[Additional note 7]
Application of the anti-aging composition according to any one of Supplementary Notes 1-5 to the production of cosmetics, wherein the cosmetics include lotions, face packs, essences, eye creams, eye packs, skin care gels, skin care emulsions, Application of an anti-aging composition, characterized in that it comprises a face cream or spray.

[Additional note 8]
The cosmetic product further includes an additive including at least one of a solvent, a thickener, a preservative, a pH adjuster, a humectant, an emollient, a chelating agent, an emulsifier, an essential oil, a fragrance, and a pigment. Application of the anti-aging composition according to appendix 7, characterized in that:

[Additional note 9]
The cosmetic product contains, in parts by weight, 12.5-100 parts of anti-aging composition, 0.1-0.8 parts of hydrogenated rapeseed oil alcohol, 5-10 parts of diisostearyl malate, and 1,3-propanediol. 5-10 parts of coconut oil alkyl caprylate, 3-12 parts of macadamia nut fatty acid phytosteryl, 0.01-0.1 part of retinol palmitate, 0.01-0.5 part of hesperidin methyl chalcone, and Application of the anti-aging composition according to appendix 8, characterized in that it is an eye cream containing 50-80 parts of ionized water.

[Additional note 10]
The method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, Application of the anti-aging composition according to appendix 9, characterized in that it comprises the step of obtaining a cream.

Claims (8)

An anti-aging composition for suppressing skin aging, comprising a nanoliposome and an active ingredient that is encapsulated in the nanoliposome and includes diglucosyl gallic acid, glucosyl hesperidin, Lacustilla chinensis extract, biotin, and caffeine. It is a thing,
In weight percentages, 0.011%-1.1% diglucosyl gallic acid, 0.01%-1.5% glucosyl hesperidin, 0.01%-0.8% Lacustrum extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 An anti-aging composition comprising .5% hydrogenated lecithin and the balance deionized water . In weight percentages, 0.011%-0.5% diglucosyl gallic acid, 0.01%-0.5% glucosyl hesperidin, 0.0447%-0.6% Laminaria japonica extract, 0.00001 %-0.0005% biotin, 0.00001%-0.0005% caffeine, 0.6%-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6 Anti-aging composition according to claim 1, characterized in that it comprises .5% hydrogenated lecithin and the balance deionized water. In weight percentages, 0.011% diglucosyl gallic acid, 0.5% glucosyl hesperidin, 0.0447% Lacustnia chinensis extract, 0.0001% biotin, 0.0001% caffeine, 0.6 %-2.6% cholesterol, 0.1%-1.5% behenyl alcohol, 6%-6.5% hydrogenated lecithin, and the balance deionized water . The anti-aging composition described in . a) A step of dissolving diglucosyl gallic acid, glucosyl hesperidin, Lacustnia chinensis extract, biotin, caffeine, cholesterol, behenyl alcohol, and hydrogenated lecithin in a solvent to obtain a mixed solution;
b) Remove the solvent in the mixed solution of step a) by evaporation, add deionized water at 40-60 °C, and perform ultrasonic treatment for 15-20 minutes at an ultrasonic frequency of 15-35 Hz. The method for producing an anti-aging composition according to any one of claims 1 to 3 , comprising the step of filtering after completing the treatment to obtain an anti-aging composition. Application of the anti-aging composition according to any one of claims 1 to 3 to the production of cosmetics, wherein the cosmetics are lotions, face packs, essences, eye creams, eye packs, skin care gels, skin care emulsions. Application of an anti-aging composition, characterized in that it comprises a face cream or a spray. The cosmetic product further includes an additive including at least one of a solvent, a thickener, a preservative, a pH adjuster, a humectant, an emollient, a chelating agent, an emulsifier, an essential oil, a fragrance, and a pigment. Application of the anti-aging composition according to claim 5 , characterized in that: The cosmetic product contains, in parts by weight, 12.5-100 parts of anti-aging composition, 0.1-0.8 parts of hydrogenated rapeseed oil alcohol, 5-10 parts of diisostearyl malate, and 1,3-propanediol. 5-10 parts of coconut oil alkyl caprylate, 3-12 parts of macadamia nut fatty acid phytosteryl, 0.01-0.1 part of retinol palmitate, 0.01-0.5 part of hesperidin methyl chalcone, and Application of the anti-aging composition according to claim 6 , characterized in that it is an eye cream containing 50-80 parts of ionized water. The method for producing the eye cream includes:
1) Add hydrogenated rapeseed oil alcohol, diisostearyl malate, retinol palmitate, coconut oil alkyl caprylate, phytosteryl macadamia nut fatty acid, and hesperidin methylchalcone, stir evenly, and heat to 50-90°C to make a uniform mixture. Stirring to form an oil phase;
2) uniformly mixing and stirring the anti-aging composition, water, and 1,3-propanediol to form an aqueous phase;
3) Mix the oil phase obtained in step 1) and the aqueous phase obtained in step 2), put the obtained mixture into the reaction pot of the supercritical device, and raise the temperature inside the reaction pot of the supercritical device. Adjust the temperature by injecting carbon dioxide to 45-65℃, set the pressure to 12-15Mpa and time to 30-45 minutes, reduce the pressure to release carbon dioxide, cool to room temperature, Application of the anti-aging composition according to claim 7 , characterized in that it comprises the step of obtaining a cream.