JP5275779B2 - Whitening agent and external preparation for skin - Google Patents

Description

  The present invention relates to a whitening agent and a skin external preparation containing the same.

As an active ingredient of a skin external preparation such as cosmetics, various plant-derived ingredients are used. For example, an extract of bark of a pine family plant is known to have an inhibitory action on tyrosinase activity and an inhibitory action on matrix metaprotease activity, and it has been proposed to be used as a skin external preparation such as cosmetics (Patent Documents 1 to 3). 4). Further, Patent Document 5 proposes a skin external preparation containing canine larch extract, and Patent Document 6 proposes a skin external preparation containing a pine family plant or a solvent extract thereof. In Patent Document 5, it is described that the “dog larch extract” is an extract of bark or root bark of dog larch, and in Patent Document 6, a trunk or bark is exemplified as a preferable use site, and according to Examples. Only the extract of the bark of the Pinaceae plant has actually been confirmed. Further, Patent Document 7 proposes a cosmetic containing a pine extract and its moisturizing action has been confirmed, but there is no description as to which part of the pine is an extract. Patent Document 8 discloses a medicinal cosmetic comprising a mixture of chlorogenic acid, pantetonic acid and the like obtained by selecting and extracting at least one kind from a plant group such as pine, and tea infusion. Has been proposed and its whitening effect has been confirmed, but there is no description as to which part of the pine extract was actually used.
Thus, although the above-mentioned patent document describes the effect of the whitening action, etc., on the extract of the bark, root bark or stem part of the pine plant, the extract of the pine cone of the pine plant There is no description specifically showing the effect of the whitening action or the like.
JP 7-196640 A Japanese Patent Laid-Open No. 10-25238 JP 2003-238425 A JP 2003-277223 A JP 2000-226323 A JP 2004-352658 A JP 2001-31521 A JP 2003-192529 A

  By the way, conventionally, various plant extracts having a whitening effect are known. From the viewpoint of obtaining a sufficient whitening effect, actually, whitening agents such as arbutin, ascorbic acid and derivatives thereof, kojic acid, and ellagic acid. Is widely used. However, although these commonly used whitening agents are excellent in whitening effect, there are many restrictions on blending into the external preparation for skin, and external preparations containing these whitening agents are discolored or colored over time, separated, In addition, various problems such as crystal precipitation are likely to occur. In addition, there are drawbacks to skin feel such as stickiness.

  The present invention has been made in view of the above problems, and can be easily blended into an external preparation for skin, and has a high whitening effect, and has a good stability, a good skin feel, and a high whitening effect. It is an object of the present invention to provide an external preparation for skin.

[MEANS TO SOLVE THE PROBLEM] In order to solve the said subject, this invention provides the whitening agent which uses the extract of the pine genus Pinus cone as an active ingredient, and the skin external preparation containing it.
The whitening agent of the present invention is a whitening agent having an ability to suppress melanin production.
Examples of the plant belonging to the genus Pinus include Pinus densiflora.
Moreover, the skin external preparation of this invention may contain the other whitening agent, As an example, 1 or more types chosen from ascorbic acid or its derivative (s), and arbutin are preferable.

ADVANTAGE OF THE INVENTION According to this invention, the whitening agent which can be easily mix | blended easily with skin external preparations widely without choosing dosage forms, such as an aqueous | water-based dosage form and an emulsifier type | mold, and has the outstanding whitening effect can be provided.
Moreover, according to this invention, while showing the outstanding whitening effect, stability is favorable, the skin feel is also favorable, and the skin external preparation for skin with a high whitening effect can be provided.

Hereinafter, the present invention will be described in detail. In the present specification, “to” means a range including numerical values before and after.
The present invention relates to a whitening agent comprising as an active ingredient an extract of a pine cone of the genus Pinaceae. As described above, the extract of other parts of the genus Pinaceae has been confirmed to inhibit tyrosinase activity. However, depending on the site, since the cytotoxicity of the extract is strong, it may be difficult to formulate at a high concentration to a degree sufficient to obtain a whitening effect due to its ability to inhibit tyrosinase activity. As a result of intensive studies by the present inventors, it has been found that an extract of the pine genus Pinus has high melanin production-inhibiting ability and low cytotoxicity. Furthermore, the extract of the pine genus Pinus spp. Is suitable for use as a skin external preparation applied to the skin because it exhibits a high whitening effect without causing unpleasant skin feeling such as stickiness. .

  As the Pinus densiflora plant, Pinus sylvestris Linne (Pinaceae) is also preferable. In the present invention, a cone of Pinaceae, a so-called pine cone extract is used. The extraction can be performed according to a general method. Specifically, it can be prepared by immersing a cone of a Pinus pine plant such as Scots pine in an extraction solvent for a predetermined time. If desired, it may be carried out under heating or pressure. Further, in order to facilitate extraction, pretreatment such as cutting the cone into small pieces and pulverizing it into powder may be performed.

  Although it does not specifically limit as an extraction solvent, For example, Water; Lower monohydric alcohols, such as methyl alcohol and ethyl alcohol; Liquid polyhydric alcohols, such as glycerol, propylene glycol, and 1, 3- butylene glycol; Ketones, such as acetone; Ethyl One or more of ethers such as ether; esters such as ethyl acetate; and the like can be used. Water, lower monohydric alcohol, and liquid polyhydric alcohol, and water-lower monohydric alcohol mixed solution and water-liquid polyhydric alcohol mixed solution are preferable. Also preferred are lower monohydric alcohols and liquid polyhydric alcohols to which about 1% urea is added.

  The extract obtained by the extraction can be used as it is as a whitening agent, or it can be used as it is after standing for aging for an appropriate period. If necessary, it can be used after being subjected to a purification treatment such as deodorization and decolorization by filtration or ion exchange resin within a range that does not affect the effect of the present invention. Further, a fraction having high activity can be taken out and used by using a separation means such as liquid chromatography.

  The extract may be in any form such as liquid, paste or gel. The liquid extract containing the extraction solvent can also be used after it is solidified by drying under reduced pressure or freeze drying. It can also be dried by spray drying or the like and used as a powder.

  Also, there are commercially available pinecone extracts of the genus Pinaceae, for example, the extract of pinus cone of Pinus densiflora under the trade names “Matsukasa extract BG” and “Betetol water-soluble pine”. Since they are variously provided, they can be used as the whitening agent of the present invention as they are or after being treated.

  The present invention also relates to an external preparation for skin containing the whitening agent of the present invention, that is, a cone extract of the genus Pinaceae as an active ingredient. Since the external preparation for skin of the present invention exhibits a high whitening effect by the cone extract, the external preparation for whitening exhibits an action of whitening the skin or preventing or improving the blackening of the skin when applied to the skin. Useful as. The content of the cone extract in the external preparation for skin is preferably 0.00001 to 1% by mass (hereinafter simply referred to as “%”), more preferably 0.0001 to 0 in terms of dry solid content. 0.1%. If it exists in this range, the said extract can be mix | blended stably and the high whitening effect can be exhibited. When the extract is used as a solution, the concentration of the extract is not limited as long as the content of the dry solid content as a solute is within the above range.

  The external preparation for skin of the present invention may contain other whitening agents together with the whitening agent of the present invention. Examples of other whitening agents include ascorbic acid or derivatives thereof, arbutin, linoleic acid, vitamin E and derivatives thereof, glycyrrhizic acid and derivatives thereof, tranexamic acid, placenta extract, chamomile extract, licorice extract, age extract , Ogon extract, seaweed extract, cucumber extract, caquette extract, gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sun penz extract, white lily extract, peonies extract, Sempukuka extract, soybean extract, tea extract, molasses extract, sandalwood extract, grape extract, hop extract, micaika extract, mokka extract, yukinoshita extract and the like. Among them, the other whitening agent is preferably one or more selected from ascorbic acid or a derivative thereof and arbutin. These whitening agents have a high whitening effect, but on the other hand, high concentration blending may be difficult in terms of stability over time and usability. By using together with the whitening agent of the present invention, it is possible to obtain a whitening effect that is more than the whitening effect of the conventional skin whitening preparation for whitening without impairing the stability over time and the feeling of use. The blending ratio of the other whitening agent and the whitening agent of the present invention (other whitening agent / whitening agent of the present invention) is preferably 3000: 1 to 1: 3000 in terms of mass ratio, and has a higher whitening effect. It is more preferable that it is 300: 1 to 1: 300 from the point obtained.

  Further, in preparing the external preparation for skin of the present invention, other active ingredients that do not impair the effects of the present invention, such as anti-aging effects, or other active ingredients that exhibit wrinkle formation suppression effects by exposure to ultraviolet rays, You may mix | blend. More specifically, examples include, but are not limited to, UV protection agents, antibacterial agents, anti-inflammatory agents, cell activators, active oxygen scavengers, and moisturizers.

  Examples of the UV protection agent include paramethoxycinnamate-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 sodium sulfate, 4-t-butyl-4′- Examples thereof include methoxydibenzoylmethane, 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, and zinc oxide.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoate ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, and the like.

  Anti-inflammatory agents have the effect of suppressing inflammation such as hot flashes and erythema after sunburn. For example, sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, Altea extract, Ashitaba extract , Arnica extract, Ginseng extract, nettle extract, Duckweed extract, Hypericum extract, Chamomile extract, Snapdragon extract, Watercress extract, Comfrey extract, Salvia extract, Bamboo extract, Perilla extract , Birch extract, gentian extract and the like.

  Cell activators are used for the purpose of improving rough skin, such as caffeine, chicken crown extract, shell extract, shell extract, royal jelly, silk protein and its degradation products or derivatives thereof, lactoferrin or degradation products thereof. , Mucopolysaccharides such as chondroitin sulfate and hyaluronic acid or their salts, collagen, yeast extract, lactic acid bacteria extract, bifidobacteria extract, fermentation metabolic extract, ginkgo biloba extract, barley extract, assembly extract, lysium extract Products, carrot extract, rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, succinic acid and the like.

  The active oxygen scavenger has an action such as suppression of lipid peroxide production. For example, superoxide dismutase, mannitol, quercetin, catechin and derivatives thereof, rutin and derivatives thereof, button pi extract, yashajitsu extract, melissa extract , Lanahan fruit extract, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivative, riboflavin and its derivative, pyridoxine and its derivative, vitamin B such as nicotinic acid and its derivative, tocopherol and its derivative, etc. Vitamin E, dibutylhydroxytoluene, butylhydroxyanisole, etc. are mentioned.

  Examples of the humectant include proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, amino acids such as glycine, serine, aspartic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, Trehalose, inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipid, ceramide, auren extract, ginger extract, diautum extract, sensyu extract Product, mallow extract, gypsophila extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

  Moreover, arbitrary components other than the whitening agent of this invention can be mix | blended with the skin external preparation of this invention. Examples of such components include, but are not limited to, physiologically active substances such as amino acids, lipids, sugars, hormones, enzymes, and nucleic acids. In addition, components that are usually used in the production of cosmetics, quasi-drugs, skin external preparations and the like, for example, water (purified water, hot spring water, deep water, etc.), oil agents, Surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, fragrance, deodorant, salt, pH adjuster, refreshing agent, plant / animal -Extracts derived from microorganisms, blood circulation promoters, astringents, antiseborrheic agents, chelating agents, keratolytic agents, enzymes, hormones, other vitamins and the like can be used as necessary.

  The external preparation for skin of the present invention has various forms such as powders such as powder and powder foundation; solids such as soap and lipstick; emulsions such as cream, emulsion and cream foundation; It is preferable that it is a cosmetic composition. However, it is not limited to these.

  Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples. Hereinafter, “part” means “part by mass” unless otherwise specified.

[Example 1: Preparation of extract of Scots pine cone]
Scots pine cones are shredded and extracted with a mixed solution of water-ethyl alcohol (containing 50 vol% ethyl alcohol), and the resulting extract is dried to a powder and extracted from Scots pine cones Got.

[Example 2 (for comparative example): Preparation of Siberian deciduous pine (parts other than cones) extract]
The formation layer and the xylem of the siberian deciduous pine sapwood were extracted in the same manner as in Example 1 to obtain an extract of siberian deciduous pine (parts other than cones).

[Example 3: Melanin production inhibitory effect (whitening rate) and cell growth rate test by cell culture]
B16 melanoma cultured cells derived from mice were used to examine the melanin production inhibitory effect and cell growth rate of the pine cone extract extracted above.
Specifically, an appropriate amount of 10 vol / vol% FBS-containing MEM medium was taken in two 6-well plates, seeded with B16 melanoma cells, and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%. The next day, the red pine cone extract prepared in Example 1 has a final concentration of 0 μg / mL (control), 40 μg / mL, 80 μg / mL, 120 μg / mL, 160 μg / mL, and 200 μg / mL with respect to the medium. Were added and mixed. On day 5 of the culture, the medium was changed, and the sample preparation solution was added again. On the next day, the medium was removed, and the cells were washed with phosphate buffer on one plate and then collected, and the degree of whiteness of the cultured B16 melanoma cells was evaluated according to the following criteria. In addition, kojic acid with known whitening effect was used as a positive control for comparison.
(Criteria)
++: A whitening effect comparable to that of a sample to which 200 ug / mL of kojic acid is added is shown.
+: A whitening effect comparable to that of the sample to which 100 ug / mL of kojic acid was added is shown.
±: A whitening effect comparable to that of the sample to which 50 ug / mL of kojic acid was added.
-: No whitening effect.
n. d. : Cell growth rate is low and cannot be determined.

For the remaining one plate, cells were fixed with formalin, added to a 1 w / vol% crystal violet solution and stained. The cell growth rate (%) with respect to each sample concentration was measured with a monocerator (Olympus).
The results are shown in the table below.

From the results of Table 1, the red pine cone extract prepared in Example 1 exhibits a remarkable whitening effect of “++” and has a low decrease in cell growth rate at a concentration exhibiting the high whitening effect. Can understand that it is an excellent whitening agent.
On the other hand, for the samples to which the Siberian deciduous pine extract prepared in Example 2 was added, the cell growth rate was too low, and the whitening effect could not be determined.

[Example 4: Preparation of pine cone extract]
Cut 10 kg of pine cones, add about 70 L of water-1,3-butylene glycol mixed solution containing 50 vol% of 1,3-butylene glycol, extract by heating, and filter to remove solid matter. . The filtrate is left to mature for more than 3 days in a cool place, and the oliage and precipitates produced in this step are removed by filtration, and further mixed with water-1,3-butylene glycol containing 50 vol% 1,3-butylene glycol. The liquid was added to obtain about 100 kg of an extract of Scots pine cones. The content of Scots pine cone extract in this extract was 0.8% in terms of dry solids.

[Example 5: Preparation of lotion 1]
A lotion sample having the composition shown in the following table was prepared.
(Manufacturing method)
A. In the following table, component No. 1-8 are mixed and dissolved.
B. In the following table, component No. 9-17 are mixed and dissolved.
C. A is added to B and mixed to obtain a lotion.

(Evaluation)
The following evaluation was performed about each prepared lotion. The results are shown in the table below.
Evaluation on whitening effect (overall observation such as whiteness, dullness, blotches):
Each sample was applied to the face twice a day in the morning and every day by 15 people, and after one month of continuous use, the skin condition was self-observed, and the whitening effect was evaluated according to the following criteria.
Effective: Significant improvement compared to before use.
Slightly effective: Improved compared to before use.
No change: No change from before use.
Deterioration: Deteriorated compared to before use.
Furthermore, the evaluation results of the subjects were statistically evaluated, and the whitening effect was evaluated for each sample according to the following criteria.
◎: Total number of effective / slightly effective is 12 or more ○: Total number of effective / slightly effective is 8 to 11 △: Total number of effective / slightly effective is 5 to 7 ×: Total number of effective / slightly effective Less than 4

Evaluation of stickiness:
Each sample was actually applied to the skin by 10 professional panelists, and the stickiness was evaluated according to the following criteria.
I do not feel any stickiness. 5 points Little stickiness. 4 points I feel a little sticky, but I don't mind. 3 points I feel sticky and a little anxious. 2 points I feel quite sticky. 1 point Furthermore, the evaluation result of the panel member was statistically evaluated, and each sample was evaluated for stickiness according to the following criteria.
◎: Average score of 10 people is 4 points or more ○: Average score of 10 people exceeds 3 points and less than 4 points △: Average score of 10 people exceeds 2 points and 3 points or less ×: Average of 10 people 2 points or less

Stability assessment:
Prepare two samples and store them for one month at 40 ° C and 5 ° C, respectively, and then visually observe the appearance of each sample (change in appearance such as coloring, discoloration, odor change, precipitation, crystal precipitation, etc.) And evaluated according to the following criteria.
A: No change is seen at all.
○: Slight change but no problem.
Δ: A change is observed, which causes a slight problem.
X: A big change is seen and it is a problem.

  The inventive products 1 to 5 were superior to the comparative products 1 to 3 in any evaluation of the whitening effect, non-stickiness, and stability.

[Example 6: Preparation of lotion 2]
(Manufacturing method)
A. The following components (1) to (7) are mixed and dissolved.
B. The following components (8) to (11) are mixed and dissolved.
C. B was added to A and mixed to obtain a skin lotion.
(Ingredient)%
(1) Citric acid 0.05
(2) Sodium citrate 0.2
(3) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(4) Glycerin 3.0
(5) 1,3-butylene glycol 8.0
(6) Scots pine cone extract ^{* 1} 0.05
(7) Purified water remaining amount (8) Ethyl alcohol 10.0
(9) Perfume Appropriate amount (10) Preservative Appropriate amount (11) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
* 1: Extract prepared in Example 4

  The prepared lotion was not sticky, had a fresh skin feel, had an excellent whitening effect, had no odor, discoloration, or precipitation, and had good stability.

[Example 7: Preparation of emulsion]
(Manufacturing method)
A. The following components (1) to (10) are dissolved by heating and maintained at 70 ° C.
B. The following components (11) to (16) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (17) is further added and mixed.
D. C is cooled, the following component (18) is added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 1.0
(2) Cetanol 0.5
(3) Lipophilic glyceryl monostearate 0.5
(4) Liquid paraffin 2.0
(5) Squalane 3.0
(6) Jojoba oil 3.0
(7) Cetyl palmitate 0.2
(8) Preservative appropriate amount (9) Sorbitan monostearate 0.3
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(11) Triethanolamine 0.5
(12) 1,3-butylene glycol 15.0
(13) Glycerin 3.0
(14) Polyethylene glycol 6000 0.5
(15) Scots pine cone extract ^{* 1} 0.1
(16) Purified water remaining amount (17) 1% solution of carboxyvinyl polymer 8.0
(18) Perfume appropriate amount * 1: Extract prepared in Example 4

  The prepared emulsion was non-sticky, had a smooth skin feel, had an excellent whitening effect, had no odor, discoloration, separation, etc., and had good stability.

[Example 8: Preparation of cream]
(Manufacturing method)
A. The following components (1) to (13) are dissolved by heating and maintained at 70 ° C.
B. The following components (14) to (19) are dissolved by heating and maintained at 70 ° C.
C. B is added to A to emulsify, and the following component (20) is further added and mixed.
D. C is cooled, and the following component (21) is added and mixed to obtain an emulsion.
(Ingredient)%
(1) Stearic acid 2.5
(2) Cetanol 2.5
(3) Lipophilic glyceryl monostearate 2.0
(4) Vaseline 2.0
(5) Dipentaerythritol fatty acid ester ^{* 1} 2.0
(6) Isotridecyl myristate 5.0
(7) Liquid paraffin 8.0
(8) Squalane 5.0
(9) Beeswax 1.0
(10) Cetyl palmitate 2.0
(11) Sorbitan sesquioleate 0.5
(12) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.5
(13) Preservative appropriate amount (14) Triethanolamine 1.2
(15) 1,3-butylene glycol 8.0
(16) Glycerin 2.0
(17) Polyethylene glycol 20000 0.5
(18) Scots pine cone extract ^{* 2} 1.0
(19) Purified water remaining amount (20) 1% aqueous solution of carboxyvinyl polymer 10.0
(21) Fragrance appropriate amount * 1: Cosmol 168AR (Nisshin Oillio Group)
* 2: Extract prepared in Example 4

  The prepared cream had no stickiness, had a smooth and rich skin feel, had an excellent whitening effect, had no odor and discoloration, and had good stability.

[Example 9: Preparation of serum]
(Manufacturing method)
A. The following components (1) to (8) are mixed and dissolved.
B. The following components (9) to (17) are mixed and dissolved.
C. A is added to B and mixed to obtain a serum.
(Ingredient)%
(1) Glyceryl tri-2-ethylhexanoate 0.1
(2) Meadow Home Oil 0.05
(3) Jojoba oil 0.05
(4) Preservative appropriate amount (5) perfume appropriate amount (6) polyoxyethylene monooleate (20E.O.)
Sorbitan 0.5
(7) Polyoxyethylene hydrogenated isostearate castor oil (50 EO) 1.5
(8) Ethyl alcohol 5.0
(9) Glycerin 4.0
(10) Dipropylene glycol 8.0
(11) 1,3-butylene glycol 8.0
(12) Sodium lactate 0.5
(13) Sodium pyrrolidonecarboxylate (50%) solution 0.5
(14) Scots pine cone extract ^{* 1} 0.5
(15) Hydroxyethyl cellulose 0.08
(16) Sodium alginate 0.05
(17) Remaining amount of purified water * 1: Extract prepared in Example 4

  The prepared essence had no stickiness, had a mild and smooth skin feel, had an excellent whitening effect, had no odor, discoloration, precipitation, and had good stability.

Example 10: Preparation of pack
(Manufacturing method)
A. The following components (1) to (6) are dissolved by heating.
B. The following components (7) to (11) are mixed and dissolved.
C. After A is cooled, B is added and mixed to obtain a pack.
(Ingredient)%
(1) Polyvinyl alcohol 12.0
(2) Methylcellulose 0.1
(3) Glycerin 3.0
(4) 1,3-butylene glycol 5.0
(5) Scots pine cone extract ^{* 1} 0.5
(6) Purified water remaining amount (7) perfume appropriate amount (8) preservative appropriate amount (9) glyceryl tri-2-ethylhexanoate 0.1
(10) Polyoxyethylene monooleate (20E.O.)
Sorbitan 1.0
(11) Ethyl alcohol 13.0
* 1: Extract prepared in Example 4

  The applied pack is moderately tensioned when applied to the skin, is not sticky after being peeled off, is fresh, has an excellent whitening effect, and has good stability without odor, discoloration, separation, etc. It was a thing.

[Example 11: Liquid foundation (O / W type)]
(Manufacturing method)
A. The following components (1) to (7) are dissolved by heating.
B. The following components (8) to (11) are added to A, mixed uniformly, and kept at 70 ° C.
C. The following components (12) to (16) are dissolved by heating and maintained at 70 ° C.
D. B is added to C and emulsified.
E. After cooling D, the following component (17) was added and mixed to obtain a liquid foundation (O / W type).
(Ingredient)%
(1) Stearic acid 2.0
(2) Cetanol 0.5
(3) Behenyl alcohol 1.0
(4) Petrolatum 2.5
(5) Liquid paraffin 5.0
(6) Self-emulsifying glyceryl monostearate 1.0
(7) Preservative appropriate amount (8) Titanium oxide 6.0
(9) Color pigment 4.0
(10) Mica 2.0
(11) Talc 4.0
(12) Carboxymethylcellulose 0.2
(13) Bentonite 0.4
(14) Scots pine cone extract ^{* 1} 0.1
(15) 1,3-butylene glycol 8.0
(16) Purified water Remaining amount (17) Perfume appropriate amount * 1: Extract prepared in Example 4

  The prepared liquid foundation had good spread, no stickiness, a beautiful finish, an excellent whitening effect, no odor and separation, and good stability.

  ADVANTAGE OF THE INVENTION According to this invention, the skin whitening agent which can be easily mix | blended with a skin external preparation, and has a high whitening effect, and the skin external preparation which has the stability containing the good, the skin feel, and a high whitening effect are provided. be able to.

Claims (4)

European red pine cones, water and 1,3-mixture or water and melanin production inhibitor as an active ingredient an extract with a mixture of ethanol and butylene glycol. For use by adding the skin external preparation, agent according to claim 1. Furthermore, the agent of Claim 1 or 2 containing 1 or more types chosen from ascorbic acid or its derivative (s), and arbutin. A method for inhibiting melanin production (excluding medical practice), comprising a step of administering to the skin an extract of a cone of Scots pine, a mixture of water and 1,3-butylene glycol or a mixture of water and ethanol.