JP4737671B2 - Agricultural / horticultural fungicide composition - Google Patents

Description

  The present invention relates to an agricultural and horticultural fungicide, and more particularly to an agricultural and horticultural fungicide composition excellent in disease control action, which contains bacterial spores belonging to the genus Bacillus and a specific chemically synthesized fungicide.

  Plant disease control methods include cultivating or physical control using crop rotation and solar heat, chemical control using chemical pesticides, control using disease resistant varieties, and biological control using antagonistic microorganisms against attenuated viruses and pathogens. Etc. Among these, research and development of chemical pesticides, especially organic synthetic fungicides, has developed remarkably until recently, many drugs having high efficacy and various actions have been developed one after another, and various application methods have also been developed. It was. Chemical control methods using these have greatly contributed to the control of plant diseases and the labor saving of control work, and are widely spread. However, due to the emergence of so-called drug-resistant bacteria in recent years, the phenomenon that the control effect by the chemical control method is reduced has been recognized in some crops and diseases and has become a problem. In addition, as crops are designated as local production areas, continuous cropping is unavoidable. As a result, the occurrence of soil-borne diseases that are difficult to control with chemical pesticides has become a serious problem. Furthermore, the method of repeatedly using a large number of chemical pesticides repeatedly releases chemical substances that do not exist in nature into the environment, so that not only drugs that are directly toxic to animals and plants, There are concerns that it will cause adverse effects.

  As described above, disease control with chemical pesticides is likely to reduce the control effect due to the emergence of resistant bacteria, and in this case, development of a new fungicide is required. In addition, for the control of diseases that are difficult to control with chemical pesticides, alternative means or other means must be taken together. Furthermore, establishment of control technology with higher safety for the environment is also desired.

  In recent years, based on this background, we reviewed the control methods that are biased toward the use of chemical pesticides, and proposed biocontrol methods using so-called microorganisms (so-called biological pesticides) that are expected to be safer from the environment. Some of them have been put into practical use. Examples of microorganisms used for controlling diseases of such agricultural and horticultural plants include filamentous fungi belonging to the genus Trichoderma, genus Gliocladium, genus Talaromyces, bacteria belonging to the genus Agrobacterium, Pseudomonas and Bacillus.

  Among the bacteria belonging to the genus Bacillus, for example, Bacillus subtilis JB3 strain (NCIB12375), Bacillus subtilis JB3.6 strain (NCIB12376), Bacillus subtilis R1 strain (NCIB12616) or mutants thereof. Antibacterial substances obtained from the above control plant diseases, microbial infections of animals and humans, and further suppress general microbial contamination, and control diseases of various agricultural and horticultural plants using cultures of the above strains Attempts have been made (Patent Document 1).

  Under such circumstances, it is conceivable to control diseases by the combined use of chemically synthesized fungicides and antagonistic microorganisms. However, chemical synthetic fungicides often adversely affect the growth of antagonistic microorganisms, and it is difficult to obtain an additive or synergistic control effect. Under such circumstances, attempts have been made to obtain a synergistic control effect by using a microorganism that antagonizes the pathogen and a chemically synthesized fungicide (for example, Patent Document 2). Attempts have also been made to obtain an inhibitory effect on resistant bacteria by using a microorganism that antagonizes pathogenic bacteria and a chemically synthesized fungicide (for example, Patent Document 3).

  In the former attempt, a fungicide composition comprising a bacterium belonging to the genus Bacillus and a chemically synthesized fungicide as in the present invention is used. However, the examples are limited to seed treatment, for example, a direct plant. There is no description of plant disease control using a synergistic control effect such as a method of spraying on a soil, a method of spraying on soil, a method of adding to water or fertilizer added to plants or soil.

  Even in the latter attempt, a technique for obtaining an inhibitory effect on resistant bacteria by using a bacterium belonging to the genus Bacillus and a chemically synthesized fungicide in the same manner as in the present invention has been disclosed. There is no description about plant disease control using the synergistic control effect by the combined use.

Further, there is no known mixture of bacteria spores belonging to the genus Bacillus and agricultural and horticultural fungicidal compounds used in the present invention.
JP-A-63-273470 JP-T 6-511258 JP-A-10-109913

  In order to meet the needs of the industry such as safety and sufficient consideration for the environment while seeking an excellent disease control effect, the present invention has a high control effect even if the amount of chemically synthesized fungicides is reduced. An object of the present invention is to provide an agricultural and horticultural fungicide composition that is safer for the environment and superior in disease control.

  As a result of intensive studies to solve the above problems, the present inventors have found that the above problems can be solved by a combination of bacterial spores belonging to the genus Bacillus and a chemically synthesized fungicide. The present invention has been completed based on this finding.

  The gist of the present invention is as follows. Agricultural and horticultural bactericidal compounds are commercially available or known as agricultural and horticultural bactericides. These compounds are published by the Japanese Agricultural Handbook (2002), published by the National Federation of Agricultural Cooperatives. Kumiai Agricultural Chemicals Directory (2005) and SHIBUYA INDEX (2005) published by the association are known.

(1) Chemically synthetic bactericidal compounds, that is, inorganic copper compounds such as basic copper sulfate, anhydrous copper sulfate, cupric hydroxide, basic copper chloride, etc., organic copper compounds such as organic copper, copper nonylphenol sulfonate, etc., inorganic Sulfur compounds such as sulfur, total sulfide sulfur, etc.Organic sulfur compounds such as dineb, manneb, propineb, thiadiazine, thiuram, polycarbamate, anilinopyrimidine compounds such as cyprodinil, pyrimethanil, mepanipyrim, phenylpyrrole compounds, Organic chlorine compounds such as fludioxonil, such as chlorothalonil, captan, triazine, fluazinam, sulfenic acid, fusalide, etc., hydrogen carbonate agents such as sodium hydrogen carbonate, potassium hydrogen carbonate, etc.Organic phosphorus compounds such as EDDP, fosetyl, torquelophos Mechi , IBP, etc., benzimidazole compounds such as carbendazim, thiophanate methyl, thiabendazole, benomyl, fuberidazole, etc., dicarboximide compounds such as iprodione, procymidone, vinclozoline, etc., azole compounds such as fenbuconazole, cimeconazole, diclobutra Sol, triticonazole, ipconazole, fluconazole, microbutanyl, penconazole, viteltanol, bromconazole, oxpoconazole, cyproconazole, difenoconazole, diniconazole, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriahol , Hexaconazole, imibenconazole, metconazole, propiconazole, cypconazole, Conazole, tetraconazole, triadimethone, triadimenol, etc., imidazole compounds such as triflumizole, prochloraz, imazalyl, pefrazoate, piperazine compounds such as triphorin, morpholine compounds such as fenpropimorph, tridemorph, fenpro Pyridine, hydroxypyrimidine compounds such as ethylimyl, dimethylimole, etc., guanidine compounds such as iminotadine acetate, iminoctadine arbesylate, guazatine, acid amide compounds such as oxycarboxyl, benzoanilide compounds such as mepronil, Acylalanine compounds such as dichromedin, flutolanil, pencyclon, furametopil, tifluzamide, etc., such as oxadixyl, metalaxyl, methoxyacrylic Rate compounds such as azoxystrobin, cresoxime methyl, metminostrobin, trifloxystrobin, picoxystrobin, pyraclostrobin, orissastrobin, quinoxaline compounds such as quinomethionate, hydroxyanilide compounds such as fenhexamide Cyanoacetamide compounds such as simoxanil, cyanoimidazole compounds such as cyazofamide, etc.Famoxadone, spiroxamine, triazoxide, pyrazophos, fluorimido, dimethomorph, iprovaricarb, fenamidone, ethaboxam, cyflufenamide, dithianone, carpropamide, probenazole, metasulfocarbamide , Pyroxylone, hydroxyisoxazole, tricyclazole, diflumetrim, Containing one or more chemical fungicides selected from nazinoxide, isoprothiolane, oxolinic acid, acibenzoral-S-methyl, quinoxyphene, benchavaricarb isopropyl, thiazinyl, and bacterial spores belonging to the genus Bacillus A feature of a fungicidal composition for agriculture and horticulture.

(2) The agricultural and horticultural fungicide composition as described above, wherein the bacterium belonging to the genus Bacillus is Bacillus subtilis.

(3) Bacteria belonging to the genus Bacillus are Bacillus sp. The agricultural and horticultural fungicide composition as described above, which is D747 (Accession No. FERM BP-8234).

(1) A plant disease control method using the agricultural and horticultural fungicide composition according to any one of (1) to (3).

  Hereinafter, the present invention will be described in detail.

<1> Spores of bacteria belonging to the genus Bacillus The agricultural and horticultural fungicide composition of the present invention contains spores obtained from a culture of bacteria belonging to the genus Bacillus as an active ingredient.

  Bacteria belonging to the genus Bacillus used in the present invention are not particularly limited as long as they belong to the genus Bacillus, but preferably include bacteria belonging to the genus Bacillus that antagonize phytopathogenic fungi, more preferably Bacillus subtilis and Bacillus sp. D747.

  This strain D747 is a strain isolated from the air in Kikukawa City, Shizuoka Prefecture, and is currently being used by the National Institute of Advanced Industrial Science and Technology, the Patent Biological Deposit Center as Bacillus sp. It is deposited internationally as D747 (accession number FERM BP-8234).

  This Bacillus sp. The bacterial properties of strain D747 are as shown below. The bacteriological properties were tested based on Bergey's Manual of Systematic Bacteriology, volume 1 (1984).

(A) Morphological properties Form: Neisseria gonorrhoeae Size: Width 1.0-1.2 μm, Length 3-5 μm
Mobility: +
Flagellar state: Periflagellate Endospore: +
Spore position: center Spore expansion: −

(B) Culture properties Colony color: White to light brown Meat broth agar plate culture: White to cream-colored colonies, wrinkled surface

(C) Physiological properties Gram stainability: +
Reduction of nitrate: +
MR test: −
VP test: +
Indole production: −
Hydrolysis of starch: +
Utilization of citric acid: +
Inorganic nitrogen source: +
Oxidase: −
Catalase: +
Growth pH
6.8, meat extract medium: +
5.7, meat extract medium: +
Growth temperature 30 ° C: +
50 ° C .: −
Growth NaCl concentration 2%: +
5%: +
7%: +
Aerobic growth: +
Anaerobic growth: −
OF test: O
Egg yolk reaction: −
Acid production from glucose: +
Acid production from mannitol: −
Acid production from L-arabinose: −
Acid production from D-xylose: −
Gas production from glucose: −
β-galactosidase: −
NaCl and KCl requirements: −

  The agricultural and horticultural fungicide composition of the present invention comprises Bacillus sp. Which can control plant diseases as described above. D747 is contained. In the plant disease control agent of the present invention, the D747 strain can be used alone, but a mutant of the D747 strain can also be used alone or together with the D747 strain. The mutant has the bacteriological characteristics of the above-mentioned D747 strain and has a plant disease control action, and is a natural mutant, a mutant using ultraviolet rays or chemical mutants, a cell fusion strain and a gene. Recombinant strains can also be used. The D747 strain contained in the plant control agent of the present invention includes mutants of the D747 strain.

  The spore used in the present invention is obtained from a culture of bacteria belonging to the genus Bacillus. Bacteria belonging to the genus Bacillus can be cultured according to the usual culture method for bacteria belonging to the genus Bacillus, such as reciprocal shaking culture, jar fermenter culture, liquid culture such as a culture tank, and solid culture. For example, in addition to a common medium such as a meat extract medium, a medium containing glucose, peptone, yeast extract, and the like can be mentioned. In addition to the liquid medium, a solid medium such as a slope medium containing agar and a plate medium may be used. The D747 strain can be grown by culturing to obtain the desired cell mass.

  Any carbon source that can be assimilated by the above strain can be used as the carbon source of the medium. For example, in addition to sugars such as glucose, galactose, lactose, sucrose, maltose, and malt extract starch hydrolysate, various synthetic or natural carbon sources that can be used by the strain D747 can be mentioned. Similarly, as a nitrogen source of the medium, various synthetic or natural products that can be used by the strain, including organic nitrogen-containing materials such as peptone, meat extract, and yeast extract, can be used. In accordance with a conventional method for culturing microorganisms, inorganic salts such as sodium chloride and phosphate, metal salts such as calcium, magnesium and iron, trace nutrients such as vitamins and amino acids can be added as necessary.

  The culture can be performed under aerobic conditions such as shaking culture and aeration culture. The culture temperature is 20-30 ° C., preferably 25-30 ° C., pH 5-8, preferably 6-7, and the culture period is 1-4 days, preferably 2-3 days.

  As a method for separating spores from a culture of bacteria belonging to the genus Bacillus obtained as described above, a method such as membrane separation, centrifugation, or filtration separation can be used. The obtained spore fraction can be used as it is for the agricultural and horticultural fungicide composition of the present invention in a state containing a certain amount of water, or by using a drying method such as freeze drying or spray drying, if necessary. It can also be used in the agricultural and horticultural fungicide composition of the present invention as a dried product.

<2> Chemically synthetic fungicide The chemically synthesized fungicide used in the present invention provides a synergistic control effect when used in combination with bacterial spores belonging to the genus Bacillus.

<3> Agricultural and horticultural fungicide composition The agricultural and horticultural fungicide composition of the present invention contains one or more spore of bacteria belonging to the genus Bacillus and one or more of the above chemically synthesized fungicides.

  The agricultural and horticultural fungicide composition of the present invention, according to a normal method for producing a microbial preparation, the spore of the bacterium belonging to the genus Bacillus and the chemical synthetic fungicide together with various optional components, if necessary, powder, granule, It can be formulated and used in wettable powders, emulsions, liquids, flowables, coatings and the like.

  Furthermore, in the plant disease control agent according to the present invention, the D747 strain is used as a cell or culture alone, diluted with an inert liquid or solid carrier, and a surfactant or other auxiliary agent is added as necessary. It may be used as a drug. Specific examples of preparations include dosage forms such as granules, powders, wettable powders, suspension preparations, and emulsions. Examples of preferred carriers include solid carriers such as talc, bentonite, clay, kaolin, diatomaceous earth, white carbon, vermiculite, slaked lime, silica sand, ammonium sulfate, urea, porous, water, isopropyl alcohol, xylene, cyclohexanone, methylnaphthalene, alkylene Examples thereof include liquid carriers such as glycol. Examples of the surfactant and dispersant include dinaphthyl methane sulfonate, alcohol sulfate ester salt, alkyl aryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, and polyoxyethylene sorbitan. Examples include monoalkylates. Examples of the auxiliary agent include carboxymethyl cellulose, polyethylene glycol, propylene glycol, gum arabic, and xanthan gum, and examples of the protective agent include skim milk and pH buffering agent. In this case, the amount of the D747 strain and / or its culture, as well as the application time and application amount, can be appropriately determined according to the case of the above live bacteria.

  The agricultural and horticultural fungicide composition of the present invention thus obtained can be stored for a long period of time regardless of the form of the preparation. For example, even after being stored as a wettable powder at room temperature for 48 months, A control value equivalent to that without a synthetic fungicide is obtained.

  The agricultural and horticultural fungicide composition of the present invention belongs to algal fungi (Omycetes), ascomycetes (Ascomycetes), basidiomycetes, Zygomycetes, and incomplete fungi (Deuteromycetes), depending on the application form. Plant diseases caused by fungi and bacteria can be controlled.

  The plant pathogens that can be controlled by the agricultural and horticultural fungicide composition of the present invention are specifically Pseudoperonospora, such as Pseudoperonospora cubensis and Venturia. Bacteria such as Venturia inaequalis, Erysiphe genus such as wheat powdery mildew, Pyricularia genus, such as rice blast fungus For example, Botrytis cinerea, Rhizoctonia ) Genus fungi such as Rhizoctonia solani, Cladosporium genus such as tomato leaf mold fungus (Cladosporum fulvum), Collototricum genus fungus such as strawberry charcot rot Puccinia genus bacteria, such as wheat red rust fungus (Puccinia recondita), Septoria genus bacteria such as Septoria nodorum, Sclerotinia nuclei, sclerotiorum), Pythium spp. Phythium debaryanum Hesse, genus Gaeumanomyces, for example, wheat stem blight (Gaeumanomyces graminis), and bacteria such as Burkolderia, for example, rice seedling However, the present invention is not limited to these examples.

<4> Method for applying agricultural and horticultural fungicide composition of the present invention The agricultural and horticultural fungicide composition of the present invention can be applied directly or diluted with water or the like. The application method as a plant disease control agent is not specifically limited, For example, the method of spraying directly to a plant, the method of spraying to soil, the method of adding to the water and fertilizer added to a plant or soil, etc. are mention | raise | lifted. In addition, since the dosage of the preparation varies depending on the target disease, target crop, application method, occurrence tendency, degree of damage, environmental conditions, dosage form used, etc., it is preferably adjusted as appropriate.

  In addition, when applying the agricultural and horticultural fungicide composition to the cultivated plant, the insecticide, nematicide, herbicide, plant growth regulator, fertilizer, soil improvement material, etc. are mixed, applied alternately or simultaneously. It is also possible.

The application amount of the agricultural and horticultural fungicide composition of the present invention varies depending on the type of disease, the type of plant to be applied, the dosage form of the fungicide composition, etc. When the composition is sprayed, the spore concentration is 10 ⁵ to 10 ¹⁰ cfu (colony forming unit) / ml, preferably 10 ⁷ to 10 ⁹ cfu / ml. Moreover, it is preferable to apply 0.5-1000 liters of the agricultural and horticultural fungicide composition as a liquid agent per 10 ares.

  The agricultural and horticultural fungicide composition of the present invention exhibits excellent control effects against various pathogens such as cucumber gray mold, downy mildew, wheat powdery mildew, rice blast, etc. Can be reduced. Also, the safety for useful crops is extremely high.

  The present invention will be specifically described by the following examples, but the present invention is not limited to these examples.

(Production Example 1: Production of wet cells)
Bacillus sp. Isolated from the air in Shizuoka Prefecture D747 strain (FERM BP-8234) was cultured on a plate medium, and the isolated colonies were inoculated into a flask. In a 20 ml broth medium (meat extract 1%, peptone 1%, sodium chloride 0.5%), 27 ° C., 120 rpm, after 1 day shaking culture, resulting culture with 1% glucose, soluble starch 2%, polypeptone 0.5%, dried yeast 1%, defatted soybean _{1% KH} 2 PO 4 0.2% After inoculating 20 L of a medium of pH 6.0 containing 0.2% NaCl and 0.3% calcium carbonate and shaking culture at 27 ° C., 120 rpm for 3 days, centrifugation (10,000 × g, 15 minutes) And suspended in sterile water to wash the medium components. This operation was performed twice to obtain wet cells (spore fraction) having a wet weight of about 1 kg. This spore fraction is a Bacillus sp. It contains 50% by weight of D747 spores by dry weight.

(Production Example 2: Production of powder spore)
Bacillus sp. Obtained in Production Example 1 above. 1 kg of the D747 spore fraction was suspended in 5 L of distilled water and then treated with a spray dryer (Niro Japan) (inlet temperature 150 ° C., outlet temperature 100 ° C.). By crushing the dried product obtained by the spray dryer, about 100 g of dry powder spores were obtained. This dry powder is Bacillus sp. D747 spores containing 5 × 10 ¹¹ cfu / g or more.

  Next, the preparation method will be specifically described with reference to typical preparation examples of the agricultural and horticultural fungicide composition of the present invention. In the following description, “%” indicates a weight percentage.

(Formulation example 1: wettable powder)
Bacillus sp. Obtained as described above. 20% powder spores of D747 strain, 1.8% of agricultural and horticultural fungicide mepanipyrim, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, clay 49 .2% was mixed and ground uniformly to obtain a wettable powder.

(Formulation example 2: wettable powder)
Bacillus sp. Obtained as described above. D747 strain powder spore 30%, agricultural and horticultural bactericidal compound fludioxonil 0.9%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 40 .1% was mixed and ground uniformly to obtain a wettable powder.

(Formulation example 3: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 30% sodium bicarbonate of agricultural and horticultural compound, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, clay 31 % Was uniformly mixed and ground to obtain a wettable powder.

(Formulation example 4: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 9% azoxystrobin, agricultural and horticultural bactericidal compound, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, clay 52% was uniformly mixed and ground to obtain a wettable powder.

(Formulation example 5: wettable powder)
Bacillus sp. Obtained as described above. D747 strain powder spore 60%, agricultural and horticultural bactericidal compound tricyclazole 0.05%, naphthalenesulfonic acid formalin condensate sodium salt 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 10 .95% was mixed and ground uniformly to obtain a wettable powder.

(Formulation Example 6: Powder)
Bacillus sp. Obtained as described above. The powder spore of D747 strain was uniformly mixed and pulverized with 10% of the powder spore, 1% of the agricultural and horticultural bactericidal compound fusaride, 6% of diatomaceous earth and 83% of clay to obtain a powder.

(Formulation Example 7: Granules)
Bacillus sp. Obtained as described above. 10% powder spores of D747 strain, 10% agricultural and horticultural bactericidal compound flutolanil, 2% sodium lauryl alcohol sulfate, 5% sodium lignin sulfonate, 2% carboxymethylcellulose and 71% clay were uniformly mixed and ground. . 20 parts by weight of water was added to 100 parts by weight of the mixture, kneaded, processed into granules of 14 to 32 mesh using an extrusion granulator, and dried to form granules.

(Formulation example 8: wettable powder)
Bacillus sp. Obtained as described above. 10% powder spore of D747 strain, 1.5% naphthalenesulfonic acid formalin condensate sodium salt, 1.5% polyoxyethylene alkylaryl, 26% diatomaceous earth, 61% clay, and uniformly pulverized, wettable powder It was.

(Formulation example 9: wettable powder)
Agricultural and horticultural bactericidal compound mepanipyrim 1.8%, sodium naphthalene sulfonic acid formalin condensate 1.5%, polyoxyethylene alkylaryl 1.5%, diatomaceous earth 26%, clay 69.2% uniformly mixed and ground. To obtain a wettable powder.

  Next, test examples show that the agricultural and horticultural fungicide composition of the present invention is useful as a plant disease control agent.

(Test Example 1: Cucumber gray mold control effect test)
Ten cucumber seeds (variety: Sagamihanjiro) were sown in 9 cm x 9 cm PVC pots, grown in a greenhouse for 7 days, and cucumber seedlings with cotyledons were used as test plants. The agricultural and horticultural fungicide composition of the present invention prepared by appropriately changing the blending amount so as to obtain the test concentration according to Formulation Examples 1 to 5, Bacillus sp. D747 and a chemically synthesized fungicide prepared according to Formulation Example 9 were diluted with water so that the active ingredient had a predetermined concentration, and 10 ml per one pot was sprayed. After air-drying, a paper disk soaked with a spore suspension of Botrytis cinerea was placed on the surface of the cucumber cotyledon and managed in a wet chamber at 20 ° C. Three days after the inoculation, the lesion diameter of the cotyledon was obtained, and the control value (%) was obtained from Equation 1 based on the obtained numerical value.

  The control effect expected when two types of active compounds and microorganisms are mixed and processed can be obtained from the calculation formula of Colby (calculation of synergistic and antagonistic responses of herbicide combinations: Calculating) Synergistic and Antagonistic Responses of Herbide Combination, Weed 15, pages 20-22, 1967). The calculation formula of Colby is shown in Formula 2.

  In Equation 2, X represents the control effect (control value) when one active compound or microorganism is treated at a concentration of m (ppm or cfu / ml), and Y represents the other active compound or microorganism n (ppm or The control effect (control value) when treated at a concentration of cfu / ml). In that case, E is the control when the former active compound or microorganism is mixed and treated so that the concentration of the latter active compound or microorganism is n (ppm or cfu / ml) and n (ppm or cfu / ml). It represents the expected value due to the additive effect (control value).

  The control effect of the agricultural and horticultural fungicide composition of the present invention is X as Bacillus sp. If the control effect obtained by treating only D747 and the control effect obtained by treating only the chemically synthesized fungicide as Y is larger than the calculated value (E) obtained from the Colby formula, the control effect by this combination is obtained. It shows that it is being demonstrated by a synergistic effect. Table 1 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

(Test Example 2: Wheat powdery mildew control effect test)
Nine wheat seeds (variety: Norin 61) were sown in a 9 cm × 9 cm PVC pot, grown in a greenhouse for 8 days, and used as a test plant. The agricultural and horticultural fungicide composition of the present invention prepared by appropriately changing the blending amount so as to obtain the test concentration according to Formulation Examples 1 to 5, Bacillus sp. D747 and a chemically synthesized fungicide prepared according to Formulation Example 9 were diluted with water so that the active ingredient had a predetermined concentration, and 10 ml was sprayed per pot. After air drying, spores of wheat powdery mildew (Erysiphe graminis) were inoculated and placed in a 20-25 ° C. greenhouse. 10 days after the inoculation, each diseased area of the first leaf was indexed according to the following criteria. Based on the obtained index value, the degree of damage was obtained by Equation 3, and the control value (%) was obtained by Equation 4. It was. Table 2 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

Disease index 0: Does not detect disease
1: Disease area less than 5% of leaf area
2: Disease area of 5% or more and less than 10% of leaf area
3: Disease area of 10% or more and less than 25% of leaf area
4: Onset area of 25% or more of leaf area

(Test Example 3: Cucumber downy mildew control effect test)
Ten cucumber seeds (variety: Sagamihanjiro) were sown in 9 cm x 9 cm PVC pots, grown in a greenhouse for 7 days, and cucumber seedlings with cotyledons were used as test plants. The agricultural and horticultural fungicide composition of the present invention prepared by changing the blending amount appropriately so as to obtain the test concentration according to Formulation Examples 1 to 5, and Bacillus sp. D747 and a chemically synthesized fungicide prepared according to Formulation Example 9 were diluted with water so that the active ingredient had a predetermined concentration, and sprayed at 10 ml per pot. After air drying, a conidial spore suspension of cucumber downy mildew (Pseudoperonospora cubensis) was spray-inoculated and immediately placed in a 20 ° C. humidity chamber for 24 hours. After that, after moving into the greenhouse, after 7 days, the degree of disease of each cotyledon was investigated according to the following criteria of disease index, and based on the obtained index value, the degree of damage was calculated by Equation 3, and the control value by Equation 4 ( %). Table 3 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

Disease index 0: Does not detect disease
1: Disease area less than 5% of leaf area
2: Onset area of 5% or more and less than 33.3% of leaf area
3: Onset area of 33.3% to less than 66.6% of leaf area
4: Disease area of 66.6% or more of leaf area, or fallen leaves

(Test Example 4: Rice Blast Control Effect Test)
About 15 seeds of rice seed (variety: Aichi Asahi) were sown in 7 cm diameter clay pots, grown in a greenhouse for 2 to 3 weeks, and rice seedlings in which the fourth leaf was completely developed were used as test plants. The agricultural and horticultural fungicide composition of the present invention prepared by changing the blending amount appropriately so as to obtain the test concentration according to Formulation Examples 1 to 5, and Bacillus sp. D747 and a chemically synthesized fungicide prepared according to Formulation Example 9 were diluted with water so that the active ingredient had a predetermined concentration, and sprayed at 10 ml per pot. After air-drying, a conidial spore suspension of Pyricularia oryzae was spray-inoculated and immediately placed in a humid chamber at 25 ° C. for 24 hours. Then, it moved to the greenhouse, and the number of lesions on the 4th leaf was investigated 5 days after the inoculation. Table 4 shows the control value (%) of the experimental value obtained by the test and the control value (%) of the calculated value obtained from the calculation formula of Colby.

As is clear from the above test examples, the agricultural and horticultural fungicide composition of the present invention has an excellent control effect due to a synergistic effect even at a concentration at which only individual antagonistic microorganisms and chemically synthesized fungicides alone show an insufficient control effect. Therefore, even if the amount of the chemically synthesized fungicide is reduced, a high control effect can be obtained.

Claims (2)

1 selected from mepanipyrim, fludioxonil, sodium bicarbonate, fenbuconazole, diniconazole, azoxystrobin, cresoxime methyl, chlorothalonil, trifloxystrobin, simoxanyl, cyazofamide, ethaboxam, iprovalib, fusaride, metminostrobin, carpropamide, tricyclazole Species or two or more chemical fungicides, and Bacillus sp. An agricultural and horticultural fungicide composition containing spores of D747 (accession number FERM BP-8234) . A plant disease control method using the agricultural and horticultural fungicide composition according to claim 1.
more than