JP2913219B2 - Urine sediment test result management method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a urine sediment inspection result management method.

[0002]

BACKGROUND OF THE INVENTION In the urine, red blood cells due to capillary bleeding, white blood cells due to extravasation, epithelial cells lining the kidney and genitourinary organs, under certain circumstances casts formed in the renal tubules, and often local infections and systemic Includes the formation of microorganisms such as bacteria, fungi, protozoa, and parasites due to infection. Also, in the case of renal failure, urine may contain crystals composed of chemical components, pharmaceuticals and free fat.

[0003] Therefore, by microscopic examination of urine, it is possible to know the concentration of these components and other components whose presence, presence and form and number are clinically significant, and to make a clear judgment on the condition of the patient. Therefore, microscopic examination of urine sediment is widely spread worldwide.

Conventional urine sediment microscopy is

As shown in FIG. 1, the urine sample 2 in the urine collection cup 1 is mixed well to make the components uniform, and
The sample is accurately collected up to the mark line 4 of
500r. p. m. After centrifugation for 5 minutes, the supernatant is removed by decantation or suction to obtain 200 μl (concentration ratio: 50 times) of urine sediment 6. Next, the urine sediment 6 is uniformly suspended, and about 15 μl is transferred to a slide glass 7, covered with a cover glass 8, set on a stage of a microscope 9, observed at 400 × magnification for particle morphology, and classified and counted. The results are manually recorded on a recording sheet.

However, the urine sample 2 has a large individual difference in the amount of urinary sediment, and is extremely thin and has only a small amount of urinary sediment.
1 (per 10 ml of urine). or,
The urine specimen 2 having a large amount of urine sediment tends to be insufficiently mixed.

In the operation method, there is a difference in the manufacturer of the Spitz tube 3 used by the inspection facility, and the centrifugal force applied by the centrifugal separator 5 used in the centrifugal separation is constant even though the rotation speed and the centrifugation time are unified. Forces vary, and the recovery of each particle in urine varies from laboratory to laboratory.

[0007] Further, if the supernatant is removed by suction removal, the amount of urine sediment can be accurately set to 200 µl. However, this method is troublesome and time-consuming. In general, decanting is performed, and the amount of urinary sediment largely reflects individual differences. For example, the amount of urinary sediment is less than 200 μl or more than 200 μl, and the concentration ratio fluctuates greatly.

Further, when urine sediment 6 is transferred to slide glass 7,

As shown in FIG. 1, it is sufficient to suspend the urine sediment 6 with the dropper 10 and collect 15 μl of the urine sediment. However, since labor and cost are required, the spitz tube 3 is lightened without using the dropper 10 in an inspection facility having a large number of samples. Shake to mix urine sediment 6,
In many cases, the Spitz tube 3 is turned upside down, and one drop of urine sediment 6 is collected by striking it on a slide glass 7. However, in such a knocking method, urine sediment 6
Suspension becomes insufficient, and the volume of the solution dropped is less than or larger than the reference volume of 15 μl, and thus varies.

As described above, urine can be easily obtained, and much diagnostic information can be obtained from the urine. On the other hand, the components are easily denatured, and it is difficult to predict what and how much is contained.
It is a difficult point that the determination easily includes an individual difference of a sample, a difference between test facilities, a difference between test personnel, and the like. Therefore, the necessity of standardization of urine sediment is recognized.

Conventionally, a method for standardizing urine sediment has not been determined, and a method using control urine for urine sediment for the purpose of quality control has been proposed. That is, healthy human blood is washed with NaCl-added phosphate buffer solution (PBS), and
Erythrocytes fixed with 5% glutaraldehyde, and leukocytes collected from the blood, similarly fixed with glutaraldehyde, were pooled with normal urine to remove formalin.
In addition to the 5% concentration, the mixture was allowed to stand overnight at 4 ° C. for over night to remove the precipitate, and a control urine was added to the pool urine obtained by adding sodium azide so as to have a constant concentration. In addition, the number of white blood cells is inspected to control the test results. (Hygiene inspection, 39 volumes, No. 1,
1990, 55-61)

[0011]

However, such a control method using control urine is effective as a means for eliminating differences between test personnel and improving reproducibility in the same laboratory, but it is effective for individual differences in urine specimens. It cannot be corrected if the amount of urine sediment varies.

When the control urine was used and tested at 58 laboratories nationwide by the routine method of each laboratory, it was found that fixed erythrocytes were 33 cells / μl (theoretical value per field of view: 12.1 cells / HPF). 2.0 to 52.7 pieces per visual field, 18.8 on average, CV 59.6 per adjusted display value
%, The inter-facility variability is extremely large, and the need for standardization is reaffirmed (Medical Examination, Volume 40,
No. 1, 1991, 75-79).

Therefore, each laboratory must work on improving the test method so that the test result using the control urine becomes the indicated value of 33 / μl (12.1 / HPF), as described above. In order to improve the accuracy of the test results of urine sediment, which often causes fluctuations, it is necessary to take time and effort, increase the cost, and limit the throughput of many samples.

[0014]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned conventional problems, and comprises adding marker particles to urine and centrifuging the urine to remove target particles and marker particles in the obtained urine sediment. The present invention relates to a urine sediment test result management method, which comprises counting and correcting the number of target particles based on the recovery rate of marker particles.

Here, the correction method is as follows.
It is on the street.

(Number of target particles counted) × (Number of theoretically recovered marker particles / Number of counted marker particles)
・ (Equation 1)

(Number of counted target particles) × (number of experimentally recovered marker particles / number of counted marker particles)
・ (Equation 2)

(Number of counted target particles / Number of counted marker particles) × (Urine concentration of added marker particles) (Equation 3)

Furthermore, it is also possible to add marker particles to urine and centrifuge the urine, count the target particles and marker particles in the resulting urine sediment, and rank urine samples according to the ratio of target particles to marker particles. . A plurality of the above correction methods may be used in combination.

The marker particles used in the urine sediment test result management method described above have a particle size of 1 to 20 μm and a specific gravity of
It is a marker particle having a particle diameter of 1.040 to 1.20 , preferably a particle diameter of 2 to 10 μm and a specific gravity of 1.05 to 1.20 . Further, the particle size of the marker particles is preferably different from the target particles in the urine sediment. Here, the target particles refer to one or more of the particles found in the urine sediment. For example, blood cell components such as red blood cells, white blood cells, histiocytes, squamous cells, migration, tubules (small circles), fat-containing cells, epithelial cells such as inclusion body cells, multinucleated giant cells, atypical cells, glass, granules, wax-like Erythrocytes, leukocytes, blood, epithelium, fat, casts such as casts, calcium oxalate, ammonium magnesium phosphate, calcium phosphate, calcium carbonate, ammonium urate, uric acid, bilirubin and other crystals, bacteria, yeast-like fungi, fat globules, There are other particles such as amorphous salts.

As the marker particles, cells of animals and plants, cells of microorganisms and the like, latex particles, microcapsules, starch particles, gelatin particles and the like can be used.

The marker particles have a particle size of 1 to 20 μm.
When the particle diameter is preferably 2 to 10 μm, the particle diameter is suitable for visual observation or counting by an automatic particle counter such as an automatic cell counter, and the specific gravity is 1.040 to 1.20, preferably 1.05 to 1.05.
When it is set to １．２1.20 , when the compound is added to urine and collected by centrifugation, the recovery rate is the same as that of target particles in urine.

When the color tone of the marker particles is different from the color tone of the target particles, identification and counting become easy. As the color tone of the marker particles, the target particles may be stained by an inspection facility by the Sternheimer method, the Sternheimer-Malvin method, etc., and therefore, a color tone different from that of the target particles stained by these is preferable. The color may be a color observed in a color other than red, purple, and blue, such as black, brown, green, and gold. Specifically, fixed stained blood cells,
Examples include stained cells, fixed stained cells, stained cells, fixed stained cells, colored latex particles, colored microcapsules, colored starch particles, colored gelatin particles, and the like.

The above marker particles have a specific gravity of urine of 1.003.
Since the specific gravity is slightly larger than 1.030, sedimentation may occur when left standing for a long time. Therefore, the specific gravity of the suspension is preferably equal to or higher than the specific gravity of the marker particles. In order to adjust the specific gravity of the suspension, a commonly used buffer may be used at a high concentration, and inorganic and organic salts, saccharose, lactose, mannitol, saccharides such as sorbitol are added. Furthermore, propylene glycol (specific gravity 1.036 to 1.040), dimethyl sulfoxide (specific gravity 1.100 to 1.105), diethylene glycol (specific gravity 1.113 to 1.123), ethylene glycol (specific gravity 1.114) To 1.117) and glycerin (specific gravity of 1.260 or more). That is, the specific gravity can be adjusted by using various substances in combination, as long as the properties of the target particles in the urine sediment and the urine test of the centrifuged supernatant are not affected. Further, the dispersibility of the marker particles can be enhanced by adding a nonionic surfactant.

As a method for producing marker particles, for example, fixed erythrocytes and a reaction solution containing 3,3'-diaminobenzidine, hydrogen peroxide solution and a surfactant in a buffer solution having a pH of 8 to 10 are mixed for a certain period of time. After washing.

As the erythrocytes, erythrocytes of animals such as rabbits, cows, pigs, and sheep can be used in addition to humans. Fixation of these erythrocytes can be performed with glutaraldehyde, formalin, or the like.

The buffer of the buffer having pH 8 to 10 is pH 8
Various buffering agents having a buffering power between 10 and 10 can be used, and tris-hydrochloric acid type, glycine-NaOH type, boric acid type and the like can be used.

In the buffer, 3,3′-diaminobenzidine and aqueous hydrogen peroxide are added, and in addition to these, a nonionic surfactant can be added.

If the concentration of the marker particles is too low, the C.I. V. If the value is too large, or if the value is too large, it takes time to count.
A range of 00 is appropriate. Within this range, the time required for the counting of marker particles can be short, and there is no large variation in the overall operation time, which does not hinder the processing of many samples.

[0030]

When marker particles are added to urine at a concentration of 5 to 500 particles / μl, for example, 50 particles / μl and centrifuged, 80 to 90% of target particles and marker particles in urine are sedimented and collected. After removing the supernatant by decantation and homogenizing the urine sediment by shaking, one drop (about 15 μl) is collected on a slide glass by a tapping method, covered with a cover glass, and inspected by microscopy. The target particles and the marker particles are averaged respectively by counting and multiple fields of view.

Assuming that the recovery rate of marker particles is 100%, the theoretical number of recovered marker particles is 18.4 per visual field (recommended method by the Japan Society of Clinical Laboratory Engineers: urine 10 ml, 1500
r. p. m. 5 minutes, aspirate supernatant, urine sediment volume 200μ
1, the amount of urine sediment collected is 15 μl), and if the average number of marker particles is 18.4, the recovery rate is 100%. For example, when the amount of urinary sediment exceeds 200 μl, the enrichment rate becomes 50 times or less, the average number of marker particles becomes 18.4 particles / HPF or less, and the recovery rate becomes 100% or less. Since the recovery is also the same, if the correction is made by the equation 1, the correction is made based on the theoretical number of recovered particles. When the amount of urine sediment is less than 200 μl, the concentration is 50 times or more in decant, the average number of marker particles is 18.4 particles / HPF or more, and the recovery is 100%.
%, But the recovery rate of the target particles is also the same, so if the correction is made by the equation 1, the correction is similarly made based on the theoretical number of recovered particles.

As a result, the individual differences of the urine specimen and the test method
Differences between inspection facilities such as inspection equipment and inspection staff are corrected and reproducibility is improved. The average number of target particles per visual field is corrected based on the theoretical number of recovered particles and standardized.

Further, since the average value of the number of collected marker particles in each laboratory is determined in advance by experiment at each laboratory, the conventional average number of collected particles in each laboratory can be used as a correction standard. Thereby, the individual difference of the urine sample is corrected without changing the criterion of the test result in each test facility, and the measurement accuracy such as reproducibility is improved.

Further, for example, marker particles may be added
When the concentration is 0 particles / μl, the number of target particles in the urine sample can be displayed by multiplying the ratio of the target particles and the marker particles in a plurality of visual fields by 50, and the urine sample can be displayed. Since the differences between individuals and differences between laboratories are corrected, it is possible to deal with the direct method.

Further, marker particles are added to urine and centrifuged. The target particles and marker particles in the obtained urine sediment are counted, and the urine specimen is ranked according to the ratio of target particles to marker particles. Inspection can be further reasonably and quickly performed. That is, in recent years, rather than expressing the number of each target particle in the urine sediment with a continuous numerical value, an appropriate section is set for the number according to the target particle, and the number of target particles in the urine sample is ranked. There is a tendency to make it easier to judge the test results and to streamline the test. Particularly, a system that reports the test results online using a computer often ranks.

The marker particles to be added have a particle size of 1 to
Within the range of 20 μm, recognition and counting are easy at the time of microscopy, and if the size is different from that of the target particles, identification becomes easier and counting by an automatic particle counter becomes possible. If the specific gravity of the marker particles is 1.04 or more, the marker particles can be collected by centrifugation. If the specific gravity is in the range of 1.06 to 1.20, the marker particles can behave together with the target particles in the urine sample during centrifugation. The rates are almost the same. Further, when the marker particles have a different color tone from the target particles under the microscope, it is easier to distinguish the marker particles from the marker particles. Further, by suspending the marker particles in a liquid having a specific gravity equal to or higher than the specific gravity of the marker particles, sedimentation due to long-term storage of the marker particles is prevented, and the suspension can be easily homogenized before addition, and can be used immediately and dispersed in urine samples. The property is also good.

[0037]

【Example】

Example 1 When marker particles obtained by coloring latex particles having an average particle diameter of 5 μm and specific gravity of 1.100 to green were adjusted to 10,000 particles / μl, and 50 μl was added to 10 ml of urine, urine 1
The number of marker particles per μl is 50. 1500r.
p. m. , Centrifuged for 5 minutes at, and the supernatant was removed by decanting. After urine sediment was suspended uniformly, a drop (approximately 15 μl) was taken on a slide glass by tapping method, and
The sample was examined under a 400-fold magnification with a cover glass of mm, and red blood cells as target particles and latex particles as marker particles were counted, respectively, and the average value of a plurality of visual fields was calculated.

The average number of red blood cells in 10 visual fields is 15.2 cells / 1.
Field of view (HPF), average marker particle number is 14.5
/ HPF. The red blood cell count in the sample urine corrected by the formula 1 was 19.3 / HPF, where the theoretical collection marker particle count by the Japanese Society of Clinical Laboratory Engineers was 18.4 / HPF.

Example 2 The same operation as in Example 1 was performed using microcapsules having an average particle diameter of 2 μl and a specific gravity of 1.080. The average number of leukocytes was 14.3 per visual field, and the average number of marker particles was 12.3.

The average number of collected marker particles per field obtained by the same operation using 10 urine samples in advance is 1
Since the number was 1.6, the white blood cell count in the urine sample corrected by the expression 2 was 13.5 / HPF.

Example 3 The same operation as in Example 1 was performed using marker particles in which latex particles having an average particle diameter of 8 μm and a specific gravity of 1.110 were colored black, and the number of marker particles per μl of urine was 40. The average number of red blood cells was 27.8 / HPF, and the average number of marker particles was 19.3 / HPF. Equation 3
The red blood cell count in the urine sample corrected by
There were six.

Example 4 The same operation as in Example 1 was performed to calculate the average ratio of the number of red blood cells to the number of marker particles. The result was 15.2: 14.5, and the number of red blood cells was 1.05 times the number of marker particles. Met.
In advance, the theoretical number of recovered marker particles per visual field is 18.
When the number of erythrocytes per field is 1 to 5, the ratio of erythrocytes to marker particles is 0.054 to 0.272, and similarly, the number of erythrocytes is 6 to 10. In this case, it is 0.326 to 0.543, and 11
0.611 to 1.087 in the case of ~ 20 pieces,
In the case of 50 pieces, it is 1.141 to 2.717 and 51 to 1
In the case of 00 pieces, it is 2.771 to 5.435, and in the case of 101 pieces or more, it becomes 5.489 or more. Therefore, since the urine sample has a red blood cell count ratio of 1.05, the red blood cell count
Rank 20 / HPF.

Example 5 Human erythrocytes are fixed with glutaraldehyde and suspended in a pH 9 Tris-HCl buffer. 250 ml of 3,3'-diaminobenzidine in 50 ml of Tris-HCl buffer of pH 9
g, 1.5 ml of 3% aqueous hydrogen peroxide, and Tween 80
Was added to make a reaction solution. The reaction solution and the fixed erythrocytes were mixed in equal amounts, washed 10 minutes later with PBS, adjusted to 10,000 cells / μl in PBS containing a preservative, floated, and stored refrigerated.

Example 6 10 ml of urine was collected into a plastic Spitz tube with a marked line, and 50 μl of the fixed stained blood cells prepared in Example 5 was added to a concentration of 50 cells per 1 μl of urine. 1500r.
p. m. After centrifugation for 5 minutes, the supernatant was removed by suction with an aspirator, the sediment volume was adjusted to 200 μl, mixed uniformly with a micropipette, 15 μl was collected on a slide glass, covered with a cover glass, and red blood cells and fixed stained blood cells were calculated.
Ten fields of view were averaged. When the above operation was repeated 10 times at the same time, the average number of red blood cells was 12.3 / HPF. V. Was 8.5%. The average number of fixed stained blood cells was 11.2 / HPF, and C.I. V. Was 8.3%. When the theoretical recovery value of fixed-stained blood cells is corrected to 18.4 cells / HPF according to Equation 1, the number of red blood cells in a urine sample is 2
0.2 / HPF.

Example 7 The average number of red blood cells and the average number of fixed stained blood cells per visual field of each urine sample were determined by the same operation as in Example 6 using 30 urine samples. The average of 30 cases of fixed stained blood cells was 13.
3 / HPF, C.I. V. Was 22.2%. The erythrocyte count of each urine sample was corrected by Equation 2 using an average value of 13.3 / HPF obtained experimentally.

Example 8 In Example 6, the Spitz tube was made of glass, the supernatant was decanted, and the urine sediment was collected by tapping. The average value of 10 red blood cell counts per visual field is 3
8.8, C.I. V. Is 24.3%, the average value of fixed stained blood cells is 39.5, C.I. V. Was 22.1%. When the theoretical recovery value was corrected to 18.4 cells / HPF using Equation 1, the average red blood cell count was 18.1 cells / HPF.

Example 9 The average number of red blood cells and the average number of fixed stained blood cells per visual field of each urine sample were determined by the same operation as in Example 8 using 30 urine samples used in Example 7. 3 of fixed stained blood cells
The average of 0 cases was 38.5 / HPF. V. Is 4
It was 4.8%. When the red blood cell count of each urine sample was corrected using Equation 2 using the average value of 38.5 / HPF obtained experimentally, it was approximated to the red blood cell count of 30 urine samples obtained in Example 7. Correction values were obtained respectively.

Example 10 In Example 8, collection of urine sediment was performed using a dropper with 15 μl.
Performed by sampling. The average value of the average of 10 red blood cell counts was 35.2 cells / HPF. V. Is 11.5%, and fixed stained blood cells have an average value of 34.0 cells / HPF and C.I. V. Is 1
0.6%. When the theoretical recovery value was corrected to Equation 1 with 18.4 cells / HPF, the average red blood cell count was 19.
0 / HPF.

Example 11 Using the 30 urine samples used in Example 7, Example 10
By the same operation as described above, the average number of red blood cells and the average number of fixed stained blood cells per visual field of each urine sample were determined. The average value of 30 cases of fixed-stained blood cells was 26.2 cells / HPF. V. Is 3
It was 9.4%. When the erythrocyte count of each urine sample was corrected according to Equation 2 using the average value 26.2 / HPF obtained experimentally, the erythrocyte count of the 30 urine samples obtained in Examples 7 and 9 was Approximate correction values were obtained respectively.

Example 12 The amount of urine collected in a urine collection cup was read using the scale of the urine collection cup, and starch particles colored by an iodostarch reaction per 18 ml of urine (18500 particles / μm)
l) was added (18.5 cells / μl urine) and the urine collection cup was shaken to confirm that the blue starch particles were uniformly suspended and the contents were uniformly mixed. 10 ml of this urine was collected in a Spitz tube, and operated in the same manner as in Example 1.

5 starch particles / HP in 10 visual fields
Since it was around F, the uniformity of urine was supported. The average number of red blood cells was 20.4 / HPF, and the average number of latex particles was 13.7. 1 theoretical recovered latex
When corrected according to Equation 1 as 8.4 / HPF, the red blood cell count was 27.4 / HPF.

According to this embodiment, by adding two types of marker particles having different sizes and / or color to the urine collection cup and the Spitz tube, it is possible to easily and surely confirm the uniformity of the components at the time of urine collection and to achieve the object. Since the particles can be corrected, not only the individual differences of the urine samples were improved, but also the reproducibility was improved and the quality of the test results was remarkably improved.

Example 13 Latex particles having an average particle diameter of 3 μm and a specific gravity of 1.050 were adjusted to 10,000 particles / μl, and 50 μl per 10 ml of urine
Was added and mixed well. This was directly counted by an automatic particle counter while distinguishing between red blood cells having a particle diameter of 7 μm and marker particles having a particle diameter of 3 μm. Marker particles have a display concentration of 50 particles / μl, whereas measured values are 49 particles / μl.
Therefore, it was confirmed that the urine was sufficiently mixed.

[0054]

As described above, according to the present invention, the following various excellent effects can be obtained.

1. ADVANTAGE OF THE INVENTION According to the method of this invention, the test result of a urine sediment can correct not only the difference between measurement persons, the difference between facilities, but also the individual difference of a urine sample, and is useful for quality control.

[0056] 2. If the number of target particles is corrected using Equation 1 based on the theoretical recovery value of marker particles per visual field, a test result that conforms to the Japanese Society of Clinical Laboratory Engineers can be obtained. Standardization becomes possible on a nationwide scale.

[0057] 3. Correcting the number of target particles using Equation 2 based on the average value of the experimental collection of marker particles per field of view can correct individual differences in urine samples without changing the conventional criteria at each laboratory. Can be.

4. If the average number of target particles per visual field and the average marker particles are determined and corrected by Equation 3, the urine concentration of target particles (units / μl urine) is obtained, and the number of target particles in urine is obtained. Can be directly displayed. Further, by using the correction in accordance with Equation 2, it is possible to standardize among the laboratories by displaying the concentration without changing the conventional judgment standard in each laboratory.

5. The urine samples can be ranked for each target particle based on the ratio between the average number of target particles and the average number of marker particles per visual field, so that counting and reporting are facilitated and rationalized.

[0060] 6. Marker particle diameter is 1-20 μm, specific gravity is 1.04
If it is set to 1.20, the recovery rate from the urine sample becomes substantially the same as that of the target particles, and it can be easily counted at the time of microscopy.

[0061] 7. If the diameter of the marker particle is different from the diameter of the target particle, the identification becomes easier, and if the color tone of the marker particle is different from that of the target particle, the identification and the counting become easier.

[Brief description of the drawings]

FIG. 1 is an explanatory view showing a urine sediment inspection method.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Urine collection cup 2 Urine specimen 3 Spitz tube 4 Marking line 5 Centrifuge 6 Urine sediment 9 Microscope 10 Dropper

Claims (11)

(57) [Claims] 1. Addition of marker particles to urine and centrifugation,
A urine sediment test result management method, wherein the target particles and marker particles in the obtained urine sediment are counted, and the number of target particles is corrected based on the recovery rate of the marker particles. 2. (Number of target particles counted) × (Number of theoretically recovered marker particles / Number of marker particles counted)
The method according to claim 1, wherein the correction is performed according to (Equation 1). 3. (Number of target particles counted) × (Number of experimentally recovered marker particles / Number of counted marker particles)
The method according to claim 1, wherein the correction is performed according to (Equation 2). 4. The urine sediment test according to claim 1, wherein the correction is performed by (number of counted target particles / number of counted marker particles) × (urine concentration of added marker particles) (Equation 3). Grade management method. 5. Addition of marker particles to urine and centrifugation,
A urine sediment test result management method comprising counting target particles and marker particles in the obtained urine sediment, and ranking urine samples according to a ratio of the target particles to the marker particles. 6. The urine sediment test result management method according to claim 1, wherein the marker particles have a particle size of 1 to 20 μm and a specific gravity of 1.040 to 1.20 . 7. The urine sediment test result management method according to claim 1, wherein the marker particles have a particle diameter different from that of the target particles. 8. The method according to claim 1, wherein the marker particles have different particle diameters from the target particles, and the counting is performed by an automatic particle counter.
3. The method for managing results of urine sediment inspection according to 3, 4, or 5. 9. The method of claim 1, 2, 3, 4 or 5, wherein the marker particles are cells, bacterial cells, latex particles, microcapsules, starch particles, gelatin particles, pollen. 10. The urine sediment test result management method according to claim 1, wherein the marker particles have a different color tone under the microscope from the target particles. 11. The marker particles are fixed stained blood cells, stained cells, fixed stained cells, stained cells, fixed stained cells, colored latex particles, colored microcapsules, colored starch particles, and colored gelatin particles. 7. The method for managing results of urine sediment inspection described in 3, 4, or 5.