JPS63243879A - Standard solution for calibrating hemocytometer - Google Patents
Standard solution for calibrating hemocytometerInfo
- Publication number
- JPS63243879A JPS63243879A JP62079087A JP7908787A JPS63243879A JP S63243879 A JPS63243879 A JP S63243879A JP 62079087 A JP62079087 A JP 62079087A JP 7908787 A JP7908787 A JP 7908787A JP S63243879 A JPS63243879 A JP S63243879A
- Authority
- JP
- Japan
- Prior art keywords
- standard solution
- hemocytometer
- calibrating
- dye
- hemoglobin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012086 standard solution Substances 0.000 title claims description 32
- 102000001554 Hemoglobins Human genes 0.000 claims abstract description 23
- 108010054147 Hemoglobins Proteins 0.000 claims abstract description 23
- 239000002245 particle Substances 0.000 claims description 13
- 239000004816 latex Substances 0.000 claims description 11
- 229920000126 latex Polymers 0.000 claims description 11
- 238000002835 absorbance Methods 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 239000000975 dye Substances 0.000 abstract description 7
- AJDUTMFFZHIJEM-UHFFFAOYSA-N n-(9,10-dioxoanthracen-1-yl)-4-[4-[[4-[4-[(9,10-dioxoanthracen-1-yl)carbamoyl]phenyl]phenyl]diazenyl]phenyl]benzamide Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C=CC=C2NC(=O)C(C=C1)=CC=C1C(C=C1)=CC=C1N=NC(C=C1)=CC=C1C(C=C1)=CC=C1C(=O)NC1=CC=CC2=C1C(=O)C1=CC=CC=C1C2=O AJDUTMFFZHIJEM-UHFFFAOYSA-N 0.000 abstract description 4
- 239000001043 yellow dye Substances 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 12
- 238000005534 hematocrit Methods 0.000 description 8
- 238000004820 blood count Methods 0.000 description 6
- 210000003743 erythrocyte Anatomy 0.000 description 6
- 239000012895 dilution Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003906 humectant Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229960005323 phenoxyethanol Drugs 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000001057 purple pigment Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000001047 purple dye Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】
［発明の目的］
（産業上の利用分野）
本発明は血球計数器を校正するために用いられる標準液
の改良に関する。DETAILED DESCRIPTION OF THE INVENTION OBJECTS OF THE INVENTION Field of Industrial Application The present invention relates to improvements in standard solutions used to calibrate hemocytometers.
（従来の技術）
従来のこの種の標準液としては、血球数、ヘモグロビン
値、ヘマトクリット値が校正できる血液を固定化した標
準血液と赤血球中のヘモグロビンを精製し調整したもの
、そして血球数またはへマドクリット値の校正としてラ
テックス粒子を用いた標準粒子がある。(Prior art) Conventional standard solutions of this type include standard blood, which is fixed blood that can be used to calibrate blood cell counts, hemoglobin values, and hematocrit values, purified and adjusted hemoglobin in red blood cells, and standard solutions that can be used to calibrate blood cell counts, hemoglobin values, and hematocrit values. There are standard particles that use latex particles to calibrate Madcrit values.
（発明が解決しようとする問題点）
しかし、ヘモグロビン値の校正に従来より用いられてき
た上記の標準液には次のような欠点がある。(Problems to be Solved by the Invention) However, the above-mentioned standard solutions conventionally used for calibrating hemoglobin values have the following drawbacks.
■保存が困難で市る：血球やヘモグロビンはタンパク質
でおるから変質しやすい。品質を保つためには低温で温
度変化の少ないところで保存する必要がある。■Difficult to store and marketable: Blood cells and hemoglobin are covered with protein and are easily deteriorated. To maintain quality, it must be stored at a low temperature with little temperature change.
■高価である：実際の血液から採取するためその標準液
は高価となる。■ Expensive: Standard solutions are expensive because they are collected from actual blood.
■廃液に有害物質を含んでいる：従来のヘモグロビン標
準液はシアンメトヘモグロビンとして封入されているも
のと、測定時にシアン化カリウムを加えシアンメトヘモ
グロビンに変えるものの２種類あるが、いずれもシアン
化カリウムが使用される。シアン化カリウムは有毒（致
死１０．１５ＣＩ）であり、この標準液の１回の使用量
におけるシアン濃度は１７ＰＰＭ 、廃液は６〜７ Ｐ
ＰＭになる。このため廃液は、シアン濃度１ ＰＰＭ以
下に分解後、中和して捨てなければならない。従って、
これらの標準液の取扱いは注意を要すると共に煩雑でお
る。■The waste liquid contains harmful substances: There are two types of conventional hemoglobin standard solutions: one is sealed as cyanmethemoglobin, and the other is converted to cyanmethemoglobin by adding potassium cyanide during measurement, but potassium cyanide is used in both cases. . Potassium cyanide is toxic (lethal 10.15 CI), and the cyanide concentration in one use of this standard solution is 17 PPM, and the waste solution has a concentration of 6 to 7 PPM.
Become a PM. For this reason, the waste liquid must be decomposed to a cyanide concentration of 1 PPM or less, neutralized, and then discarded. Therefore,
Handling of these standard solutions requires care and is complicated.
尚、シアン化カリウムを用いるのは、ヘモグロビン値の
国際標準法としてシアンメトヘモグロビン法が採用され
ているからである。Note that potassium cyanide is used because the cyanmethemoglobin method is adopted as an international standard method for measuring hemoglobin values.
本発明はこのような従来の標準液の欠点に鑑みなされた
もので、その目的は有害物質を含まず、保存、取扱いが
容易な血球計数器校正用の標準液を提供することである
。The present invention was made in view of the drawbacks of the conventional standard solutions, and its purpose is to provide a standard solution for calibrating a hematology cell counter that does not contain harmful substances and is easy to store and handle.
［発明の構成］
（問題点を解決するための手段）
第１の発明では、ヘモグロビン値の測定機能を有する血
球計数器を校正するための標準液において、５２０〜５
８０ ｎｍの範囲に吸光度のピークを有する色素を有効
成分としている。[Structure of the Invention] (Means for Solving the Problems) In the first invention, in a standard solution for calibrating a hemocytometer having a hemoglobin value measurement function, 520-5
The active ingredient is a dye that has an absorbance peak in the 80 nm range.
第２の発明では、上記第１の発明の標準液に所定の大き
さのラテックス粒子を含ませている。In a second invention, latex particles of a predetermined size are included in the standard solution of the first invention.
（作用）
第１の発明について：ヘモグロビン値の校正において、
標準液に要求される条件は波長５４０ｎｍ付近の吸光度
が安定していることである。また、−例として紫色色素
と黄色色素を用いれば波長４５０〜６００ｎｍでシアン
メトヘモグロビンの吸光波形に似せることもできる。本
発明の一例として標準液とシアンメトヘモグロビンから
成る標準夫々の光波長と吸光度の関係を第１図に示す。(Function) Regarding the first invention: In the calibration of hemoglobin value,
The standard solution is required to have stable absorbance around a wavelength of 540 nm. For example, if a violet dye and a yellow dye are used, the absorption waveform of cyanmethemoglobin can be made to resemble that of cyanmethemoglobin at a wavelength of 450 to 600 nm. As an example of the present invention, FIG. 1 shows the relationship between light wavelength and absorbance of a standard solution and a standard made of cyanmethemoglobin.
第２の発明について：この標準液は所定の大きさのラテ
ックス粒子を有してあり、この粒子の大きざを例えば赤
血球の大きざとしておくならば赤血球の数やヘマトクリ
ット値の校正を行なうことができると共に第１の発明の
標準液と同様にヘモグロビン値の校正を行なうことがで
きる。Regarding the second invention: This standard solution has latex particles of a predetermined size, and if the particle size is set to the size of red blood cells, for example, the number of red blood cells and hematocrit value can be calibrated. In addition, hemoglobin values can be calibrated similarly to the standard solution of the first invention.
（実施例） 次に第１の発明の一実施例の成分を示す。(Example) Next, components of an embodiment of the first invention will be shown.
紫色色素 ０．０１１ａ／Ｊｌ（
黄色色素） ０．０３Ｍ１プロピ
レングリコール ５０ｍ ｌ ／ １工チレン
グリコールモノフエニルエーテル３ｍ１／Ｉ
ＥＤＴＡ ・２Ｎａ Ｏ１ｇ／ｌ（ノ
ニオン系界面活性剤＞ １ｍ１／１上記の紫色
色素量は、ヘモグロビン値を１４〜１６ｃ＋／ｄｆの範
囲内でに校正するために決定したものである。Purple pigment 0.011a/Jl (
Yellow dye) 0.03M1 propylene glycol 50ml / 1-functional tylene glycol monophenyl ether 3m1/I EDTA ・2Na O1g/l (nonionic surfactant > 1m1/1 The amount of purple pigment above increases the hemoglobin value from 14 to 16c+ This was determined in order to calibrate within the range of /df.
プロピレングリコール、エチレングリコールモノフェニ
ルエーテル、ＥＤＴＡ・２Ｎａはそれぞれ保湿剤、防腐
剤、キレート剤として加えられている。Propylene glycol, ethylene glycol monophenyl ether, and EDTA/2Na are added as humectants, preservatives, and chelating agents, respectively.
上記の成分量は希釈操作を行なわず直接使用することを
前提としたものでおるが、成分口を変えることにより希
釈（例えば２００倍）して校正に使用する標準液を作成
することも可能でおる。The above component amounts are based on the assumption that it will be used directly without dilution, but it is also possible to dilute (for example, 200 times) by changing the component ports to create a standard solution for use in calibration. is.
希釈タイプの標準液を作成するには、希釈操作を行なわ
ないタイプの標準液における色素）農度を希釈倍率倍す
れば良い。例えば希釈倍率２００倍のときは、それぞれ
を２００倍すれば良い。この希釈タイプの標準液を使用
する際には、希釈器で希釈した後、その希釈した標準液
により校正を行なうことになる。To create a diluted type standard solution, it is sufficient to multiply the pigment yield in a type of standard solution that does not require dilution by the dilution ratio. For example, when the dilution ratio is 200 times, each of the amounts may be multiplied by 200 times. When using this diluted standard solution, it must be diluted with a diluter and then calibrated using the diluted standard solution.
次に第２の発明の一実施例の成分を示す。Next, components of an embodiment of the second invention will be shown.
紫色色素 ２．２Ｍ １黄色色
素 Ｃｒ、ＯＶ１プロピレング
リコール ５０ｍ Ａ ／ １工チレングリコ
ールモノフエニルエーテル３ｍ Ａ ／ Ｉ
ＥＤＴＡ ・２Ｎａ ０．１ｇ／ｉ４
．９μφラテックス粒子 ２．２５Ｘ １０１０個／
１２．２μφラテックス粒子 １．５ ＸＩＯ”個／
ｌこの溶液は２００倍に希釈した後、各測定値を校正す
るように調整されたものでおる。上記各色素の量は、ヘ
モグロビン値を１４〜１６ｑ／ｄ １２に校正するため
に決定したものでおる。Purple dye 2.2M 1 Yellow dye Cr, OV1 Propylene glycol 50m A/1-functional tylene glycol monophenyl ether 3m A/I EDTA ・2Na 0.1g/i4
．． 9μφ latex particles 2.25X 1010 pieces/
12.2 μφ latex particles 1.5 XIO” pieces/
This solution was diluted 200 times and then adjusted to calibrate each measured value. The amounts of each of the above dyes were determined to calibrate the hemoglobin value to 14-16q/d12.
プロピレングリコール、エチレングリコールモノフェニ
ルエーテル、ＥＤＴＡ・２Ｎａはそれぞれ保湿剤、防腐
剤、キレート剤として加えられている。ラテックス粒子
は、その大きざが、４．９μφ、２．２μφの２種類の
ものが用いられている。Propylene glycol, ethylene glycol monophenyl ether, and EDTA/2Na are added as humectants, preservatives, and chelating agents, respectively. Two types of latex particles are used, each having a size of 4.9 μφ and 2.2 μφ.
尚、上記成分量を変えることにより、希釈操作なしで使
用できる標準液を作ることもできる。この直接使用する
タイプの標準液を作るには、上記の希釈タイプの標準液
中にお（ブる２つの色素および２種類のラテックス粒子
の濃度を、それぞれ１ ／２００倍すれば良い。By changing the amounts of the above components, it is also possible to create a standard solution that can be used without dilution. To prepare this type of standard solution for direct use, the concentrations of the two dyes and the two types of latex particles in the above-mentioned diluted standard solution can be multiplied by 1/200.
上記標準液を用いた各測定値の校正方法を以下に示す。The method for calibrating each measured value using the above standard solution is shown below.
■白血球数、赤血球数は４．９μφラテックス粒子の数
で校正する。■The white blood cell count and red blood cell count are calibrated using the number of 4.9μφ latex particles.
■血小板数は、２．２μφラテックス粒子の数で校正す
る。■The platelet count is calibrated by the number of 2.2μφ latex particles.
■ヘマトクリット値は４．９μφラテックス粒子が占め
る体積で校正する。■The hematocrit value is calibrated by the volume occupied by 4.9μφ latex particles.
■ヘモグロビン値は、この標準液における波長５４０ｎ
ｍの吸光度で校正する。■The hemoglobin value is the wavelength of 540n in this standard solution.
Calibrate with the absorbance of m.
■平均赤血球容積は、上記■、■でそれぞれ校正した赤
血球数およびヘマトクリット値を次式に代入することに
より校正する。(2) Calibrate the mean corpuscular volume by substituting the red blood cell count and hematocrit value calibrated in (1) and (2) above into the following equation.
平均赤血球容積（ｆＬ＞＝ （ヘマトクリット値（％）
／赤血球＠（百万個））ＸＩＯ
■平均赤平均赤血球ヘモグロビン上記■、■てそれぞれ
校正した赤血球数およびヘモグロビン値を次式に代入す
ることにより校正する。Mean corpuscular volume (fL>= (hematocrit value (%)
/Red blood cells@(million cells))
平均赤血球ヘモグロビン１ｆｆ（１）Ｃ１）＝（ヘモグ
ロビン値（ｇ／ｄ １ ） ／赤血球数（百万個））ｘ
ｌＯ■平均赤血球ヘモグロビン濃度は、上記■、■でそ
れぞれ校正したヘマトクリット値およびヘモグロビン値
を次式に代入することにより校正する。Average corpuscular hemoglobin 1ff (1) C1) = (hemoglobin value (g/d 1 ) / red blood cell count (millions)) x
The lO2 average corpuscular hemoglobin concentration is calibrated by substituting the hematocrit value and hemoglobin value, respectively calibrated in the above 1 and 2, into the following equation.
平均赤血球ヘモグロビン８度（（］／ｄ １ ） ＝（
ヘモグロビン値（ＣＩ／Ｌ！ ） ／ヘマトクリット値
（％））ｘｌｏｏ
［発明の効果］
本発明によれば、保存か容易でかつ有毒物質を含まず、
取扱いが容易な血球計数器校正用の標準液を実現するこ
とができる。Average corpuscular hemoglobin 8 degrees ((]/d 1 ) = (
Hemoglobin value (CI/L!) / Hematocrit value (%))
It is possible to realize a standard solution for calibrating a blood cell counter that is easy to handle.
第１図は本発明の詳細な説明図でおる。 代理人 弁理士 本 １） 崇 口１図 FIG. 1 is a detailed explanatory diagram of the present invention. Agent Patent Attorney Book 1) Takashi Mouth 1 diagram
Claims (2)
校正するための血球計数器校正用標準液において、５２
０〜５６０ｎｍの範囲に吸光度のピークを有する色素を
有効成分とすることを特徴とする血球計数器校正用標準
液。(1) In a hemocytometer calibration standard solution for calibrating a hemocytometer having a hemoglobin value measurement function, 52
A standard solution for calibrating a hemocytometer, characterized in that the active ingredient is a dye having an absorbance peak in the range of 0 to 560 nm.
校正するための血球計数器校正用標準液において、５２
０〜５６０ｎｍの範囲に吸光度のピークを有する色素と
、所定の大きさのラテックス粒子とを有効成分とするこ
とを特徴とする血球計数器校正用標準液。(2) In a hemocytometer calibration standard solution for calibrating a hemocytometer having a function of measuring hemoglobin values, 52
A standard solution for calibrating a hemocytometer, characterized in that the active ingredients are a dye having an absorbance peak in the range of 0 to 560 nm and latex particles of a predetermined size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62079087A JPS63243879A (en) | 1987-03-31 | 1987-03-31 | Standard solution for calibrating hemocytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62079087A JPS63243879A (en) | 1987-03-31 | 1987-03-31 | Standard solution for calibrating hemocytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63243879A true JPS63243879A (en) | 1988-10-11 |
Family
ID=13680099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62079087A Pending JPS63243879A (en) | 1987-03-31 | 1987-03-31 | Standard solution for calibrating hemocytometer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63243879A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS646865A (en) * | 1987-06-30 | 1989-01-11 | Japan Synthetic Rubber Co Ltd | Standard liquid for calibration of blood measuring instrument |
| JPH04278460A (en) * | 1991-03-06 | 1992-10-05 | Shima Kenkyusho:Kk | Method for controlling examination result of urine sediment, control marker particle and preparation thereof |
| JPH05135A (en) * | 1991-02-15 | 1993-01-08 | Nippon Koden Corp | Blood model |
| EP0965841A1 (en) * | 1998-04-06 | 1999-12-22 | AVL Medical Instruments AG | Control or calibration standard for instruments for the optical determination of hemoglobin concentration in blood samples |
| WO2007132903A1 (en) * | 2006-05-16 | 2007-11-22 | Arkray, Inc. | Method for automatically identifying control liquid |
| JP2008201968A (en) * | 2007-02-22 | 2008-09-04 | Asahi Kasei Pharma Kk | Method of stabilizing leuco pigment |
| US8080423B2 (en) | 2004-08-05 | 2011-12-20 | Asahi Kasei Pharma Corporation | Reagent containing protease reaction promoter and/or colorant stabilizer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4940928A (en) * | 1972-08-24 | 1974-04-17 | ||
| JPS6027861A (en) * | 1983-07-21 | 1985-02-12 | コ−ニング・グラス・ワ−クス | Co-oximetry characteristic control composition |
| JPS62194461A (en) * | 1986-02-20 | 1987-08-26 | Fuji Photo Film Co Ltd | Standard solution for dry analytical element for whole blood |
-
1987
- 1987-03-31 JP JP62079087A patent/JPS63243879A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4940928A (en) * | 1972-08-24 | 1974-04-17 | ||
| JPS6027861A (en) * | 1983-07-21 | 1985-02-12 | コ−ニング・グラス・ワ−クス | Co-oximetry characteristic control composition |
| JPS62194461A (en) * | 1986-02-20 | 1987-08-26 | Fuji Photo Film Co Ltd | Standard solution for dry analytical element for whole blood |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS646865A (en) * | 1987-06-30 | 1989-01-11 | Japan Synthetic Rubber Co Ltd | Standard liquid for calibration of blood measuring instrument |
| JPH05135A (en) * | 1991-02-15 | 1993-01-08 | Nippon Koden Corp | Blood model |
| JP2683848B2 (en) * | 1991-02-15 | 1997-12-03 | 日本光電工業株式会社 | Blood model |
| JPH04278460A (en) * | 1991-03-06 | 1992-10-05 | Shima Kenkyusho:Kk | Method for controlling examination result of urine sediment, control marker particle and preparation thereof |
| EP0965841A1 (en) * | 1998-04-06 | 1999-12-22 | AVL Medical Instruments AG | Control or calibration standard for instruments for the optical determination of hemoglobin concentration in blood samples |
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