JP2618347B2 - Push-pull probe sampling device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus for continuously fractionating and collecting biological substances near a probe by connecting a push-pull probe inserted into a living tissue to a dialysate supply end and a sampling liquid suction end. Things.

[0002]

2. Description of the Related Art Living tissue is composed of cells.
The area around the cells is filled with biological fluid, and a wide range of biological substances (low molecular weight substances, peptite,
Enzymes, proteins, etc.) play various important roles in vivo. Therefore, a push-pull probe is used as a useful tool for investigating the movement of these substances.

[0003] As the name implies, a push-pull probe supplies (pushes) a dialysate to a probe inserted into a living body, and conveys a biological fluid collected at the tip of the probe using the dialysate, and actively aspirates it. The biological material is collected by (pulling), and a push syringe 1 and a pull syringe 2 are used as shown in FIG. In this configuration, the probe 3 is sucked at substantially the same flow rate from the pull syringe 2 with respect to the liquid supply amount (flow rate) from the push syringe 1.
The fluid inflow and outflow in the vicinity of the distal end of the dialysis fluid are balanced to prevent pressurization of the tissue 4 due to oversupply of the dialysate, and to prevent decompression due to excessive suction. By operating the push-pull probe without increasing or decreasing the pressure on the tissue in this manner, the extracellular fluid can be collected in a steady state without destruction of the tissue.

However, in such a conventional push-pull probe sampling apparatus, in order to fractionate and collect the liquid collected by the pull syringe at regular intervals, the push-pull operation is stopped each time and the liquid is removed from the pull syringe. Must be taken out. But,
Once the operation is stopped in such a push-pull flow path, it takes a predetermined time (about 5 minutes) to equilibrate the hydraulic pressure in this flow path, and during this time the liquid supply becomes excessive. Pressurize the tissue, which will adversely affect the tissue cells. In this push-pull flow path, when operated using a syringe of exactly the same volume for push and pull, a part of the supplied liquid stays in the living body in exchange for the collection of biological fluid, and the There are drawbacks such as the inflow and outflow cannot be balanced.

[0005]

DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned problems and provides a biological fluid collected by the action of a suction means such as a push syringe without affecting the collection function and the tissue to be examined. And to provide a sampling device in which the push-pull operation itself is executed while maintaining the balance of the liquid inflow and outflow.

[0006]

SUMMARY OF THE INVENTION Accordingly, the present invention provides
A liquid feeding means for connecting to a liquid feeding conduit extended from a push-pull probe for collecting biological material inserted into the test tissue, and a measuring tube for connecting to a suction conduit extending from the push-pull probe. A flow path switching valve provided, and when the flow path switching valve is at the sampling position, suction means connected to the suction conduit via the measuring pipe,
When the flow path switching valve is at the liquid recovery position, by providing the liquid to be collected pushing out means and a separation flow path outlet connected to each other via the measuring pipe, the separation flow at the liquid recovery position is provided. The sampling device of the push-pull probe is configured so that a liquid to be collected can be fractionated and collected from a passage outlet in a flow path separated from the suction conduit and the suction means.

In a preferred aspect of the present invention, the liquid sending means comprises a first syringe, and the suction means comprises a second syringe having substantially the same volume as the first syringe, and a second syringe having substantially the same volume as the first syringe. And a very small capacity auxiliary cylinder.

In a further preferred aspect of the present invention, the liquid to be sampled is further provided with another syringe, a preparation position for allowing the syringe to once inhale the solvent, and the sucked solvent in the measuring tube. A second flow path switching valve having a fractionation position for feeding in, wherein the first flow path switching valve, the second flow path switching valve, and all the syringes are driven by a timer. It is.

[0009]

According to the above arrangement, by using the sampling flow path switching valve, the liquid to be collected in the measuring tube can be fractionated and collected without affecting the push-pull flow path. By the configuration with the addition of a syringe, it is possible to completely balance the liquid inflow and outflow,
These sampling and fractionation operations can be automated by timer control.

[0010]

FIG. 2 is a diagram showing an embodiment of the present invention.
In this embodiment, each of the push syringe 11 and the pull syringe 12 is a gas-tight syringe having a capacity of 5 ml, and an auxiliary pull syringe 13 composed of a gas-tight syringe having a capacity of 100 μl is arranged next to the pull syringe 12. The push syringe 11 is connected to the beginning of a liquid feeding conduit 14 extending from the push-pull probe 3, and the suction conduit 15 drawn from the push-pull probe 3 is terminated at one end of a flow switching valve 16 composed of a six-way valve. An inlet channel 17 for the pull syringe 12 is connected to a channel port b connected to the channel port a and adjacent to the channel port a of the channel switching valve 16. The above-mentioned auxiliary pull syringe 13 is also connected to the inlet channel 17. The other two flow ports e and d of the flow path switching valve 16 are inserted and connected to the fractionation flow path 18. The fractionation channel 18 is connected to the sample pushing syringe 20 through another channel switching valve 19 composed of a three-way solenoid valve, and is connected to the channel port e.
The sample is pushed out from the channel port d for fractionation, and can be discharged to the container 18a placed below the outlet of the fractionation channel 18. A conduit from the extruding solvent tank 21 is connected to another one of the flow path switching valves 19. Since the flow path switching valve 19 has the T flow path cock, it can selectively occupy the extruded solvent suction position and the sample pushed out position according to the angular position of the cock.

First flow path switching valve 1 comprising a six-way valve
The six-port cock No. 6 has three connecting flow paths indicated by solid arcs in the drawing and a measuring pipe 22 connected between the two connecting flow paths, and the measuring pipe 22 is used for suction as shown in FIG. The sampling position connected between the conduit 15 and the inlet channel 17 of the pull syringe and the fraction collection position where the measuring tube 22 is inserted into the fraction channel 18 can be selectively occupied. The flow path switching valve 16, the second flow path switching valve 19, and the syringes 11, 12, and 13 are timing driven by a timer 23. In the illustrated centering position, the syringes 11 and 12 are driven synchronously by the same motor (not shown), and the auxiliary pull syringe 13 is driven simultaneously by another motor (not shown). In the embodiment, the push syringe 11 and the pull syringe 12
Is driven at a flow rate of 3 μl / min, and the auxiliary pull syringe 13 is driven to correct at a flow rate of 0.03 μl / min, so that the liquid inflow / outflow amount at the tip of the probe 3 can be kept constant for a long time.

The ratio of the capacity of the push / pull syringe to the capacity of the auxiliary pull syringe need not be limited to the above-mentioned 50: 1, but the latter capacity can be increased to about 10: 1.

[0013]

As described above, the present invention provides a push-pull sampling device which is extremely useful in medical and other experiments in which a biological fluid can be fractionated and collected for a long time without adversely affecting the collection function or the test tissue. Is provided.

[Brief description of the drawings]

FIG. 1 is a schematic diagram illustrating a method of using a conventional push-pull probe.

FIG. 2 is a diagram showing an embodiment of a push-pull sampling device of the present invention.

[Explanation of symbols]

 REFERENCE SIGNS LIST 11 push syringe 12 pull syringe 13 auxiliary pull syringe 14 liquid feeding conduit 15 suction conduit 16, 19 flow path switching valve 17 inlet flow path 18 fractionation flow path 20 sample extruding syringe 21 extruding solvent tank 22 measuring pipe 23 timer

Claims (3)

(57) [Claims] 1. A liquid feeding means for connecting to a liquid feeding conduit extending from a push-pull probe for collecting biological material inserted into a test tissue, and a suction conduit extending from the push-pull probe. A flow switching valve provided with a measuring pipe, a suction unit connected to the suction conduit via the measuring pipe when the flow switching valve is at the sampling position, and When in the collection position, by providing the liquid to be sampled and the separation channel outlet connected to each other via the measuring pipe, the sample liquid is collected from the separation channel outlet at the liquid collection position. ,
A sampling device for a push-pull probe, wherein a fraction can be collected in a flow path separated from the suction conduit and the suction means. 2. The liquid supply means comprises a first syringe, and the suction means comprises a second syringe having substantially the same volume as the first syringe, and an auxiliary cylinder having an extremely small volume as compared with these syringes. The sampling device according to claim 1, comprising: 3. The liquid to be sampled pushing means further includes another syringe, a preparation position for allowing the syringe to once suck the solvent, and a fractionating position for feeding the sucked solvent into the measuring tube. And a second flow path switching valve, wherein the first flow path switching valve, the second flow path switching valve, and all the syringes are driven by a timer. A sampling device as described.