JP2019034902A - Composition - Google Patents

Abstract

To provide a composition capable of being used for weight reduction and cosmetic effects, capable of achieving muscle enhancement and metabolism improvement and being used always.SOLUTION: There is provided a composition including a Lagerstroemia speciosa processed product, a maca processed product, and palo azul processed product for solving problems. According to the invention, improvement, mitigation, recovery, treatment or prevention of body weight gain, abnormality of skin and various kinds of diseases and symptoms caused by them, for example, a life habit disease such as obesity (metabolic syndrome), diabetes, lipid abnormality, exercise function reduction symptom such as a joint pain, and a symptom related to a cosmetic aspect such as wrinkles and loosening of skin, can be expected.SELECTED DRAWING: Figure 1

Description

The present invention relates to a composition. In particular, the present invention relates to a composition selected from the group consisting of a muscle building composition, a diet composition and a cosmetic composition.

Due to changes in lifestyle and dietary habits, the number of people suffering from lifestyle-related diseases such as obesity (metabolic syndrome), diabetes, dyslipidemia, and their reserves are increasing. Therefore, in order to prevent or ameliorate lifestyle-related diseases, diet therapy such as diet restriction or fasting, exercise therapy for promoting energy consumption by exercise, and diets that combine these are performed.

In the diet, dietary therapy prevents overeating, avoids intake of foods high in carbohydrates and lipids, and exercise therapy increases metabolism. However, it is difficult to balance diet and exercise therapy, and it is difficult to achieve both diets efficiently.

In addition, when muscle function is reduced due to aging, lack of exercise, or inability to exercise, a decrease in muscle mass may lead to cosmetic problems such as wrinkles and sagging. Therefore, as an agent that prevents or improves or reduces muscle function deterioration such as muscle mass reduction, muscle degradation, muscle fatigue, and lowering of exercise ability, it has a muscle strengthening action, and it is effective for diet and beauty. Compositions containing are desired.

On the other hand, recently, many plants have been studied as raw materials for so-called health foods. For example, Banaba [Lagerstromia Speciosa, Linn. Or Pers. ] Has been used as a folk medicine since it has been decocted in Southeast Asia and Australia for a long time and has been eaten as a beverage. Focusing on this banaba leaf, an anti-diabetic agent comprising a concentrated dry product (banaba extract powder extract) obtained by extracting banaba leaf with hot water or an organic solvent has been proposed (Patent Document 1).

Japanese Patent Laid-Open No. 5-310587

Controlling the nutrients ingested by diet as in the conventional diet, trying to improve muscles and improve metabolism through exercise therapy is a heavy burden and has both temporal and spatial limitations. Take it.

Therefore, an efficient diet by strengthening muscles and improving metabolism is desired without requiring intake restriction by diet therapy. In particular, for such a diet, an oral composition that can be used on a daily basis, which can strengthen muscles and can fundamentally and continuously improve metabolism, is useful. However, no suitable oral composition has been known so far, and diets that have established fundamental and continuous metabolic improvements are still difficult to achieve.

Moreover, by improving muscle strengthening and metabolism, a slimming effect that tightens the body as a diet effect can be obtained, and a cosmetic effect that improves skin tightening, wrinkles, sagging, and the like can be obtained.

Then, this invention makes it the subject which this invention tends to solve to provide the composition which can be provided for a diet, beauty, etc. and established the muscle reinforcement | strengthening and the metabolic improvement which can be used regularly.

In order to solve the above-mentioned problems, the present inventors have intensively studied various ingredients, particularly ingredients having food experience, and surprisingly, banaba processed products, maca processed products, and Paloisul processed products. When combined, the effect of increasing the number of myoblasts, which was hardly seen with each component alone, was significantly increased by the combination. This indicates that the combination of the banaba processed product, the maca processed product, and the Paloastle processed product provides a very excellent myoblast number increasing effect that can be said to be synergistic rather than additive.

Moreover, surprisingly, the AMPK activating action that was not seen with each of the above components alone was significantly increased by combining the above components. It has been found that, by combining a banaba processed product, a maca processed product, and a Paloastle processed product, a very excellent AMPK activation action that can be said to be synergistic rather than additive is obtained.

It is known that AMPK is activated under the condition that intracellular ATP level decreases, and functions as a “metabolic sensor” that promotes lipid and carbohydrate metabolism and promotes ATP synthesis. AMPK is not only regulated by the intracellular energy level as described above, but is also considered to be an intracellular mediator of fatty acid oxidation and glucose utilization promoting action in addition to muscle exercise (Nature, Vol. 415, PP339-343, 2002, Nature, Vol.423, PP762-769, 2003, J. Clin. Invest., Vol.108, PP1167-1174, 2001). Here, AMPK can suppress the activity of ACC and reduce the amount of malonyl CoA, which is a product of ACC, by phosphorylating (Ser79) acetyl CoA carboxylase (ACC). As a result, it is considered that carnitine palmitoyltransferase (CPT-1) is not strongly inhibited by malonyl CoA, the activity of CPT-1 is enhanced, long chain fatty acids are taken into mitochondria, and fatty acid oxidation is promoted. Yes. Therefore, activation of AMPK contributes to energy metabolism activation such as lipid metabolism promotion, carbohydrate metabolism and fat accumulation suppression, and prevention and improvement of lifestyle-related diseases such as obesity and diabetes.

On the other hand, AMPK is an enzyme that is activated during exercise such that it is activated by phosphorylation in a situation where the intracellular AMP / ATP ratio increases, such as skeletal muscle exercise. Therefore, energy can be produced from sugars and fats that can obtain a pseudo exercise effect by AMPK activation.

As a result of the above, the present inventors can increase the number of muscle cells themselves, particularly the number of muscle cells, and also have an AMPK activating action, and therefore have a muscle enhancing action including an increase in muscle mass. As a composition that can be used for muscular energy production promotion, slimming promotion, anti-obesity, shape-up, diet and beauty, etc. by promoting metabolism, all processed banaba processed products, maca processed products, and Paloisul processing We have succeeded in creating a composition containing a combination of products. The present invention has been completed on the basis of such knowledge and successful examples.

Therefore, according to the present invention, the following composition [1] is provided.
[1] A composition comprising a processed banaba product, a processed maca product, and a processed paloassle product.

In the composition of one embodiment of the present invention, since the components are all ingredients that are experienced in eating and are safe when ingested, the number of muscle cells per se increases by combining each component, so that the amount of muscle tissue Can be increased and energy consumption can be increased fundamentally and sustainably. The composition of one embodiment of the present invention can activate each factor regulated by AMPK by activating AMPK in cells of the body such as muscle cells and fat cells. The composition of one embodiment of the present invention has such an action, so that it can exert a dieting effect and a cosmetic effect through a metabolic improving action, and can increase body weight, abnormal skin, and various symptoms and diseases caused by these. For example, it can be expected to improve, alleviate, recover, treat or prevent lifestyle-related diseases such as obesity (metabolic syndrome), diabetes and dyslipidemia, and cosmetic symptoms such as wrinkles and sagging.

FIG. 1 is a diagram showing the results of evaluating the effect of increasing the number of myoblasts using each test substance and control solution, as described in the examples described later. FIG. 2 is a diagram showing the results of evaluating the AMPK activity of myoblasts using each test substance and control solution as described in the examples described later. FIG. 3 is a diagram showing the results of evaluating the AMPK activity of adipocytes using each test substance and control solution as described in the examples described later.

Hereinafter, the composition of this invention is demonstrated based on the preferable embodiment. Hereinafter, when referring to the composition of the present invention, it applies to any of “oral composition”, “oral preparation”, “food composition”, “food composition”, and “food product”, and the present invention is safe. “Composition for increasing the number of myoblasts”, “Composition for AMPK activity”, “Composition for muscle enhancement”, “Composition for promoting muscle energy production”, “Composition for promoting slimming” , “Anti-obesity composition”, “Shape-up composition”, “Beauty composition”, “Diet composition”, “Muscle reduction prevention composition”, “Locomo syndrome prevention and / or improvement” It applies to any of the “compositions”. It should be noted that the technical scope of the present invention is not limited only to the matters of this item, and the present invention can take various forms as long as the object is achieved.

The composition which is one embodiment of the present invention preferably contains a processed banaba product, a processed maca product, and a processed Paloisul product as components useful for increasing the number of myoblasts and improving metabolism.

Banaba (Lagerstroemeria Speciosa, Linn. Or Pers.) Used as a raw material of the composition of the present invention is a plant belonging to the family Myrtaceae, commonly referred to as “Ovanasarisuberi”, including the Philippines, India, Malaysia, This plant grows widely in Southeast Asia such as South China and Australia. In the present invention, banaba flowers, leaves, bark, roots or seeds can be used, but it is preferable to use leaves from the viewpoint of muscle strengthening, dieting or beauty. Banaba leaves may contain stems. Since banaba leaves are susceptible to corrosion, it is preferable to dry them immediately after harvesting to obtain dried products. Fresh leaves may be dried either naturally or air-dried, but preferably forcedly dried by air-drying. Drying is performed so that the water content is 20% by mass or less, preferably 10% by mass or less, thereby preventing the corrosion of microorganisms and keeping the components in the banaba leaf stably.

Examples of the processed banaba used in the present invention include dry chips, pulverized products, juices, extracts, and dry powders thereof. The processed banaba used in the present invention is easy to apply in consideration of the formulation properties when used in a powdery, granular, tablet-like, or capsule-like composition, which is a preferred application form of the present invention described later. Therefore, it is preferably a pulverized product, a squeezed product or an extract, and more preferably an extract. The processed banaba may be manufactured by a method generally known by those skilled in the art or may be distributed on the market.

The method for obtaining the banaba pulverized product is not particularly limited. For example, the pulverized apparatus can be used to obtain a processed product obtained after being washed or dried by using a sun or a dryer, as it is or after being cut into an appropriate shape or size. It can obtain by grind | pulverizing using. As the pulverizer, those usually used can be widely used. For example, a pulverizer constituted by a raw material hopper, a pulverizer, a classifier, a product holder and the like can be used.

The method of obtaining the banaba juice is not particularly limited, and examples thereof include a method of squeezing banaba or a fragmented product thereof, a method of centrifuging or filtering the banaba fragmented product, and the like. As an example of a specific method for producing a squeezed product, squeezed by a mechanical crushing means such as a mixer or a juicer, and if necessary, squeezed liquid by removing crude solids by means such as sieving or filtration. The method of obtaining is mentioned. The squeezed product may be concentrated as necessary, or may be processed into freeze powder by subjecting it to freeze drying, hot air drying, spray drying or the like.

The method for obtaining the banaba extract is not particularly limited. For example, an extract obtained by extracting the components contained in the banaba leaf with a solvent according to a conventional method, a diluted solution or a concentrated solution thereof, or a dried product thereof, The powder etc. are mentioned.

Examples of the solvent used for extraction include water; lower alcohols such as ethanol, methanol, isopropanol, and butanol; lower esters such as ethyl acetate and methyl acetate; acetone; a mixed solvent of these with water. In the present invention, a mixed solvent of an organic solvent and water can be used. As the mixed solvent, for example, a mixed solvent of ethanol, methanol, isopropanol, butanol, ethyl acetate, methyl acetate, acetone, and water is used. Preferably, an acetone / water (2/8 to 8/2, volume ratio) mixture, an ethanol / water (2/8 to 8/2, volume ratio) mixture, and the like can be given. The temperature of the extraction solvent can be appropriately set between room temperature and the boiling point or less depending on the solvent used.

In the extraction method, for example, a solvent having a mass of 2 to 20 times, preferably 5 to 15 times, more preferably 8 to 10 times the mass of banaba is added, preferably 50 ° C. or more, more preferably about 50 to 85. Examples of the method include extraction within a range of several minutes to several tens of hours, preferably about 30 minutes to 2 hours, and extraction under arbitrary conditions such as standing, shaking, stirring, and reflux. In the case of extraction at a high temperature, it is preferable to perform extraction by heating and reflux because the organic solvent (for example, ethanol) may evaporate and the extraction efficiency may decrease. After the extraction operation, it is preferable to perform solid-liquid separation operations such as filtration and centrifugation to remove insoluble solid matter. An extract can be obtained by performing operations such as dilution and concentration as necessary. Further, the same operation may be repeated for insoluble matter, and the extract may be used in combination with the previous extract. These extracts may be used after further purification by a purification method commonly used by those skilled in the art.

A method for obtaining a dried product from the extract is not particularly limited, and examples thereof include a method of subjecting the extract and its concentrate to a drying process commonly used by those skilled in the art, such as spray drying, freeze drying, drying under reduced pressure, and fluidized drying. It is done. Furthermore, the dried product thus obtained can be used after being pulverized by a method known to those skilled in the art. For example, a banaba extract can be obtained according to the method described in JP-A-2005-263650. According to this method, banaba leaves are subjected to (a) an extraction step with an aqueous ethanol solution, (b) a treatment step with activated carbon, and (c) a step of concentrating and recovering a precipitate, thereby containing corosolic acid in a high content. Banaba extract can be obtained.

The content of the processed banaba is not particularly limited as long as the effect of increasing the number of myoblasts and / or the AMPK activating effect is recognized. For example, the ratio of the amount of corosolic acid to the total amount of the processed product (corosolic acid content) is 0.01 to 50% (W / W), preferably 0.1 to 40% (W / W), more preferably 0.5 to 30% (W / W), still more preferably 1 to 20% (W / W).

The raw material of the processed maca used in the present invention is a Brassicaceae Repidium genus plant, and any part such as whole grass, flowers, fruits, leaves, stems including bulbs, bulbs, etc. may be used, but muscle augmentation, diet Or it is preferable to use a bulb part from a viewpoint of beauty. For example, the components contained in maca include nitrogen-containing compounds in addition to minerals and amino acids, and examples of nitrogen-containing compounds include alkaloids.

Examples of processed maca used in the present invention include dried chips, slices, grated, pulverized products, juices, extracts, and dry powders thereof. The processed maca product used in the present invention is easy to apply in consideration of the formulation properties when used in a powdery, granular, tablet-like, or capsule-like composition which is a preferred form of use of the present invention described later. Therefore, it is preferably a pulverized product, a squeezed product or an extract, and more preferably an extract. The processed maca product may be manufactured by a method generally known by those skilled in the art or may be distributed on the market.

The maca pulverized product can be obtained by, for example, pulverizing a processed product obtained by washing with sun or a dryer after washing as it is or by cutting into a suitable shape or size using a pulverizer. Can be obtained. As the pulverizer, those usually used can be widely used. For example, a pulverizer constituted by a raw material hopper, a pulverizer, a classifier, a product holder and the like can be used.

Maca's squeezed product can be produced, for example, in the same manner as banaba squeezed product, and the obtained squeezed product may be concentrated if necessary, and may be processed by freeze drying, hot air drying, spray drying, etc. To obtain a dry powder.

The extract of maca is not particularly limited as long as it is an extract of maca components. For example, an extract obtained by extracting a component contained in banaba with a solvent according to a conventional method, a diluted solution or a concentrated solution thereof Or a dried product thereof or a powder thereof.

Examples of the solvent used for extraction include water; lower alcohols such as ethanol, methanol, isopropanol, and butanol; lower esters such as ethyl acetate and methyl acetate; acetone; a mixed solvent of these with water. In the present invention, a mixed solvent of an organic solvent and water can be used. As the mixed solvent, for example, a mixed solvent of ethanol, methanol, isopropanol, butanol, ethyl acetate, methyl acetate, acetone, and water is used. Preferably, an acetone / water (2/8 to 8/2, volume ratio) mixture, an ethanol / water (2/8 to 8/2, volume ratio) mixture, and the like can be given. The temperature of the extraction solvent can be appropriately set between room temperature and the boiling point or less depending on the solvent used. After the extraction operation, it is preferable to perform solid-liquid separation operations such as filtration and centrifugation to remove insoluble solid matter. An extract can be obtained by performing operations such as dilution and concentration as necessary. Further, the same operation may be repeated for insoluble matter, and the extract may be used in combination with the previous extract. These extracts may be used after further purification by a purification method commonly used by those skilled in the art.

A method for obtaining a dried product from the extract is not particularly limited, and examples thereof include a method of subjecting the extract and its concentrate to a drying process commonly used by those skilled in the art, such as spray drying, freeze drying, drying under reduced pressure, and fluidized drying. It is done. Furthermore, the dried product thus obtained can be used after being pulverized by a method known to those skilled in the art.

The processed product of Paloisul used in the present invention is Cyclolepis genistoids Don., A perennial plant belonging to the family Asteraceae native to the Republic of Paraguay in South America. (General name: Palo Azul) is used as a raw material. As a part of the plant body, any part such as an aerial part such as a stem, a leaf or a branch, or an underground part such as a rhizome may be used, but from the viewpoint of muscle strengthening, diet or beauty, a stem, a leaf, a branch or the like. It is preferable to use the above-ground part. The processed paloassle may be manufactured by a method generally known by those skilled in the art or may be commercially available.

Examples of the processed Palo Athlet used in the present invention include dry chips, pulverized products, juices, extracts, and dry powders thereof. The processed banaba used in the present invention is easy to apply in consideration of the formulation properties when used in a powdery, granular, tablet-like, or capsule-like composition, which is a preferred application form of the present invention described later. Therefore, it is preferably a pulverized product, a squeezed product or an extract, and more preferably an extract.

The pulverized product of Palo Aisle can be obtained by, for example, pulverizing a processed product obtained after being washed or dried by using a sun or a dryer as it is or by cutting into a suitable shape or size using a pulverizer. Can be obtained. As the pulverizer, those usually used can be widely used. For example, a pulverizer constituted by a raw material hopper, a pulverizer, a classifier, a product holder and the like can be used.

The squeezed product of Paloisul can be manufactured, for example, in the same manner as the squeezed product of banaba, and the obtained squeezed product may be concentrated as necessary, or may be treated by freeze drying, hot air drying, spray drying, or the like. To obtain a dry powder.

The extract of Palo Aisle is not particularly limited as long as the Palo Aisle component is extracted. For example, the extract obtained by extracting the component contained in the above-ground part of Palo Aisle with a solvent according to a conventional method, and its diluted solution Or a concentrated solution thereof, or a dried product thereof or a powder thereof.

Examples of the solvent used for extraction include water; lower alcohols such as ethanol, methanol, isopropanol, and butanol; lower esters such as ethyl acetate and methyl acetate; acetone; a mixed solvent of these with water. In the present invention, a mixed solvent of an organic solvent and water can be used. As the mixed solvent, for example, a mixed solvent of ethanol, methanol, isopropanol, butanol, ethyl acetate, methyl acetate, acetone, and water is used. Preferably, an acetone / water (2/8 to 8/2, volume ratio) mixture, an ethanol / water (2/8 to 8/2, volume ratio) mixture, and the like can be given. The temperature of the extraction solvent can be appropriately set between room temperature and the boiling point or less depending on the solvent used. After the extraction operation, it is preferable to perform solid-liquid separation operations such as filtration and centrifugation to remove insoluble solid matter. An extract can be obtained by performing operations such as dilution and concentration as necessary. Further, the same operation may be repeated for insoluble matter, and the extract may be used in combination with the previous extract. These extracts may be used after further purification by a purification method commonly used by those skilled in the art.

A method for obtaining a dried product from the extract is not particularly limited, and examples thereof include a method of subjecting the extract and its concentrate to a drying process commonly used by those skilled in the art, such as spray drying, freeze drying, drying under reduced pressure, and fluidized drying. It is done. Furthermore, the dried product thus obtained can be used after being pulverized by a method known to those skilled in the art.

The composition of one embodiment of the present invention has a myoblast number increasing action and / or an AMPK activating action by containing the processed banaba, processed maca, and processed paloass. It has muscle strengthening effect, diet effect and beauty effect through its action.

The “muscle enhancement effect” in the present specification refers to, for example, an increase in the number of myoblasts and / or a muscle enhancement effect due to an increase in muscle mass through activation of AMPK, a promotion of muscle energy production by an improvement in metabolism, etc. The effect of use. The “diet effect” in the present specification refers to an effect such as an increase in the number of myoblasts and / or prevention of slimming and weight gain by improving metabolism through AMPK activation. The “beauty effect” in the present specification refers to, for example, an improvement, relaxation, or recovery of skin tissue including facial muscles such as skin tightening wrinkles and sagging through the action of increasing the number of myoblasts and / or AMPK activation. , Refers to treatment or prevention.

The method for producing the composition of one embodiment of the present invention is not particularly limited. For example, the composition can be made into a powdery, granular, solid or capsule composition by containing each component at room temperature or under heating. be able to. Alternatively, this composition can be dissolved in a solvent such as water to form a liquid composition. Furthermore, it can be set as a liquid composition by adding and mixing another solid component to the liquid material of each component. In addition, you may pulverize the obtained liquid composition through a drying process. Examples of the drying process in this case include spray drying and freeze drying, but are not limited thereto.

In the composition of a specific embodiment of the present invention, the content of the processed banaba product, processed maca product, and processed paloass product is not particularly limited. For example, the mass ratio ([processed banaba product]: [processed maca product] ]: [Processed Palo Aisle]) is 1: 0.001-50: 0.001-50, preferably 1: 0.01-30: 0.01-30, more preferably 1: 0. 1-10: 0.1-10.

As a specific embodiment of the composition of the present invention, a composition containing 1 to 98 wt% of the processed Paloassule, 1 to 98 wt% of the processed banaba, and 1 to 98 wt% of the processed macaque can be mentioned.

The composition of one embodiment of the present invention can be used in various forms that are expected to have a muscle strengthening effect, a diet effect and a cosmetic effect. For example, you may utilize for various purposes, without adding a special process. When the composition of one embodiment of the present invention is used for oral use, the dosage form is not particularly limited. For example, the pharmaceutical composition for muscle strengthening, dieting or cosmetic, quasi-drug composition, and food and beverage composition Or the like.

The specific one aspect | mode of the food-drinks composition is a functional food-drinks which are food-drinks which have fixed functionality with respect to a biological body, for example. Functional foods and beverages include, for example, specific health foods and drinks, functional indication foods and drinks, nutritional functional foods and drinks, health functional foods and drinks, special purpose foods and drinks, nutritional supplements and foods, health supplements and foods, supplements, beauty foods and beverages In addition to so-called healthy foods and beverages such as foods and beverages for infants, foods and beverages for pregnant women, foods and beverages for specific people such as foods and beverages for elderly people are included. Furthermore, functional food / beverage products include the health food / beverage products to which the health claim based on the food standards of Codex (FAO / WHO Joint Food Standards Committee) is applied.

The amount of intake of the composition of one embodiment of the present invention is not particularly limited, and can be appropriately set according to the degree of muscle strengthening, diet, and beauty required by the intaker, the intake mode, etc. The total amount of the processed banaba, processed maca and processed paloass is 0.1 to 10,000 mg / kg, preferably 0.5 to 1,000 mg / kg, particularly preferably based on the weight of the intake person. 1-100 mg / kg.

The daily intake of the composition of one embodiment of the present invention is not particularly limited. For example, the total amount of processed banaba, processed maca and processed paloass is 0.01 to 10 g, preferably 0.05 to 5 g. Yes, particularly preferably 0.1 to 1 g.

As described above, the utilization form of the composition of one embodiment of the present invention is not particularly limited, and for example, it can be an oral composition. The form of the oral composition is not particularly limited, and can be any form. As the form of the oral composition, for example, a form suitable for oral intake, specifically, liquid, powder, granule, granule, tablet, rod, plate, block, solid, round, paste , Capsules like hard capsules and soft capsules, caplets, tablets, gels, etc., but can be ingested easily, so it is in the form of powder, granules, tablets, capsules, tablets It is more preferable.

The composition of one embodiment of the present invention can be a container-packed composition sealed in a container. Although the container is not particularly limited, for example, metal such as aluminum, paper, plastic such as PET or PTP, single layer or laminated film bag, retort pouch, vacuum pack, aluminum container, plastic container, bottle, can, etc. The packaging container is mentioned. In order to avoid deterioration over time, the composition of one embodiment of the present invention is preferably one that has been sterilized by pressurization and / or heating after being packed in a container and sealed.

The method of using the composition of one embodiment of the present invention is not particularly limited. For example, the composition of one embodiment of the present invention is orally ingested by eating and drinking, as it is, together with water, or diluted with water. can do. The composition of one embodiment of the present invention may be mixed with another solid or liquid and taken orally according to the taste of the user. When the composition of one embodiment of the present invention is made into an orally disintegrating dosage form, it can be taken orally without water.

The composition of one embodiment of the present invention only needs to contain at least a processed banaba product, a processed maca product, and a processed paroassule, and these may be combined with other components. Although it does not specifically limit as another component, For example, a thickener, a brightener, a manufacturing agent, etc. can be used as another component. In addition to these, various excipients, binders, lubricants, stabilizers, diluents, extenders, emulsifiers, colorants, fragrances, perfume oils and the like can be used as other components. The content of other components can be appropriately selected depending on the form of the composition of one embodiment of the present invention.

The composition of one embodiment of the present invention uses the muscle strengthening effect, diet effect, and / or cosmetic effect through its myoblast number increasing action and AMPK activating action. It is useful for maintaining the health of persons suffering from symptoms or diseases caused by abnormalities and those at risk. In addition, it is useful for maintaining the health of persons suffering from other symptoms or diseases caused by weight gain or skin abnormality directly or indirectly and those at risk. Furthermore, the composition of one embodiment of the present invention is useful for healthy persons who feel metabolic deterioration and skin aging, particularly middle-aged persons and those who lack exercise. Furthermore, the composition of one embodiment of the present invention is particularly interested in muscle enhancement, beauty by strengthening facial muscles, collagen production, skin turnover activation by increasing muscle mass and activating skin metabolism. It is also useful for healthy people from young to middle-aged.

Specifically, the muscle strengthening composition of the present invention includes pharmaceuticals (including quasi drugs) and so-called health foods. In so-called health foods, “muscle strengthening”, “shape up”, “muscles” Examples of the display may include “recovery from fatigue”, “improvement of muscle fatigue”, “prevention of bedridden”, “prevention of bedridden”, “locomo care”, and the like. The subject of ingesting the oral composition of the present invention is not particularly limited as long as it is a person who needs muscle strengthening, but a person aiming to shape up, an athlete, an elderly person with weak legs and the like, etc. It can be illustrated preferably.

Specifically, the diet composition of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods. In so-called health foods, “to those who are concerned about body fat”, “obesity” Examples of those that display "For those who are uncomfortable", "For those who are concerned about body weight (BMI)", "Reduce body weight and stomach fat (visceral and subcutaneous fat)", "Reduce waist circumference", etc. can do.

Specifically, the cosmetic composition of the present invention includes pharmaceuticals (including quasi-drugs) and so-called health foods. In so-called health foods, “skin condition”, “skin tone, Examples include those that display “Keep gloss”, “Tighten skin”, “Improve skin wrinkles and sagging”, and the like.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples, and the present invention can take various modes as long as the problems of the present invention can be solved. .

As described below, the effect of increasing the number of myoblasts and the effect of activating AMPK by the combination of the processed banaba, processed maca, and processed paloass were evaluated.

[Example 1. Increases the number of myoblasts]
(1) As the test substance banaba processed product, a commercially available powdered banaba leaf hydrous ethanol extract was used. Further, as the processed maca, a commercially available powdered maca root hydrous ethanol extract was used. As the processed Paloisul product, a commercially available powdered Paloasl above-ground hydrous ethanol hot water extract was used.

(2) By preparing a solution in which each test substance is dissolved in test sample DMSO to a concentration of 60 mg / mL, the solution is dissolved in 2% (w / v) BSA-KRPH buffer. The test sample was prepared so that the final concentration of each test substance was the value shown in Table 1 (μg / mL). Further, a solution containing only 2% (w / v) BSA-KRPH buffer without containing a test substance was used as a control.

(3) Cell culture The mouse skeletal muscle-derived myoblast cell line C2C12 was cultured in a 75 cm ² flask containing 10 vol% FBS-DMEM medium at 37 ° C in a 5% CO ₂ incubator.

After the culture, cells suspended by trypsin treatment were collected from the 75 cm ² flask, and the number of cells was counted. Based on the measurement results, C2C12 cells were seeded at a cell density of 10,000 cells / well in each well of a collagen-coated 96-well plate. The 96-well plate seeded with cells was pre-cultured in a 37 ° C., 5% CO ₂ incubator for 3 days.

After culturing, the medium in the wells was removed, and 100 μL each of the test samples prepared in Example 1, and Comparative Examples 1 to 3 whose concentrations were adjusted as shown in Table 1 were added to each well. _The cells were cultured for 24 hours in _two incubators.

(4) Cell number measurement After the culture, the medium was removed, and each well was washed once with PBS 200 μL / well. Subsequently, 150 μL / well of Cell Counting Kit-8 solution diluted 30-fold with serum-free DMEM was added.

After the addition, the plate was allowed to stand in a 37 ° C., 5% CO ₂ incubator for appropriate color development, and then the absorbance at 450 nm of each well was measured. Moreover, in order to correct | amend the attenuation | damping of the light absorbency by a cell, the light absorbency in 650 nm was also measured and corrected. Based on the obtained data, the ratio of the number of cells to the control (% of control) was calculated based on the following formula, and the rate of change relative to the control was calculated.
% Of control = (Data sample-Data blank) / (Data control-Data blank) × 100
Rate of change (%) = Data sample (% of control) −Data control (% of control)
Data sample: Absorbance (450 nm-650 nm) of Example 1 and Comparative Examples 1 to 3
Data control: Absorbance of control (450 nm-650 nm)
Data blank: Blank when there is no cell (450 nm-650 nm)

(5) FIG. 1 below shows a summary of the calculation results of the ratio of the number of cells in Example 1 and Comparative Examples 1 to 3, with 0 as the evaluation control. In addition, the thing whose value is lower than control was described as 0.

As shown in FIG. 1, the comparison between Comparative Examples 1 to 3 and Example 1 showed that the number of myoblasts did not increase with each component alone, whereas the processed banaba and maca processed The combination of the product and the processed Paloassule increased the number of myoblasts.

From the above results, it was found that those containing the processed banaba, processed maca and processed paloass showed an excellent myoblast increasing action.

[Example 2. AMPK activation action of myoblasts]
(1) The test substance banaba processed product, maca processed product, Paloastle processed product, and control were all the same as those used in the myoblast number increasing action of Example 1 above.

(2) By preparing a solution in which each test substance is dissolved in test sample DMSO to a concentration of 60 mg / mL, the solution is dissolved in 2 (w / v)% BSA-DMEM, Test samples were prepared so that the final concentration of each test substance was the value shown in Table 2 (μg / mL). Further, a 2% (w / v) BSA-DMEM solution containing no test substance was used as a control.

(3) Myoblast cell culture The mouse skeletal muscle-derived myoblast cell line C2C12 was cultured using a 75 cm ² flask containing 10 vol% FBS-DMEM medium in a 37 ° C., 5% CO ₂ incubator. After the culture, cells suspended by trypsin treatment were collected from the 75 cm ² flask, and the number of cells was counted. Subsequently, each well of a collagen-coated 24-well plate was seeded at a cell density of 50,000 cells / well. The 24-well plate seeded with the cells was pre-cultured in a 37 ° C., 5% CO ₂ incubator for 3 days. After culturing, the medium in the well was removed, and then differentiation medium (2 vol% HS-DMEM) was added to induce differentiation into myotube cells, and the medium was changed every 2 to 3 days at 37 ° C., 5% The cells were cultured for 7 days in a CO ₂ incubator.

After culturing, the wells were washed once with 2% (w / v) BSA-DMEM medium, and 500 μL of each test sample of control, Example 2 and Comparative Examples 4 to 6 was added to each well.

(4) Measurement of AMPK activity Each cell of (3) above was cultured for 1 hour, the culture supernatant was removed, and each well was washed twice with ice-cold PBS. Then, 200 μl / well of RIPA buffer containing 1% protease inhibitor and 20 mM sodium fluoride was added to lyse the cells. This cell lysate was collected in a 1.5 mL tube, centrifuged at 13,000 rpm for 3 minutes, and the supernatant was collected to obtain a sample. The protein concentration of the resulting sample was measured using a BCA protein assay kit. The sample, RIPA buffer, 4 × Laemmli Sample Buffer were mixed, heated at 95 ° C. for 5 minutes, allowed to cool to room temperature, and then electrophoresed. The protein used for electrophoresis was adjusted to 4 μg / well. Proteins were transferred from the gel after electrophoresis to a PVDF membrane.

The mixture was blocked with 5% BSA-TBST buffer by shaking at room temperature for 1 hour. After soaking in 5% BSA-TBST containing primary antibodies (anti-total AMPK rabbit monoclonal antibody and anti-phosphorylated AMPK rabbit monoclonal antibody), shaking overnight at 4 ° C., washing with TBST for 5 minutes × 3 times, and then secondary antibody ( HRP-conjugated anti-rabbit IgG goat polyclonal antibody) and shaken at room temperature for 1 hour. After washing with TBST, chemiluminescence measurement was performed. The developed area was digitized with imaging software, phosphorylated AMPK (pAMPK) in total AMPK (tAMPK) was calculated, and calculated as a ratio to the control. FIG. 2 shows the results using the above myoblasts.

FIG. 2 shows that in myoblasts, an increase in AMPK activity was observed in the group in which all the components were combined as compared to the individual component group, indicating that the composition of the present invention activates AMPK. It was.

[Example 3. AMPK activation action of adipocytes]
(1) Test substance and test sample Table 3 below shows the concentration (μg / mL) of each test sample prepared in the same manner using the same test substance as the myoblast test substance in Example 2 above. Show.

(2) Cell culture of adipocytes Mouse-derived fibroblast 3T3-L1 was cultured in a 75 cm ² flask containing 10 vol% FBS-DMEM medium in a 37 ° C., 5% CO ₂ incubator. Cells suspended by trypsin treatment were collected from a 75 cm ² flask, counted, and then seeded at a cell density of 60,000 cells / well in each well of a collagen-coated 24-well plate. The 24-well plate seeded with the cells was pre-cultured in a 37 ° C., 5% CO ₂ incubator for 3 days.

After culturing, the medium in the well was removed, and in order to induce differentiation, the cells were cultured in a differentiation induction medium for 3 days and in a differentiation maintenance medium for 4 days. While changing the medium every 1 to 3 days, the cells were cultured in a 37 ° C., 5% CO ₂ incubator for a total of 7 days to induce differentiation into adipocytes. After the wells were washed once with 2% (w / v) BSA-DMEM medium, 500 μL of each test sample of control, Example 3 and Comparative Examples 7 to 9 was added to each well. The differentiation-inducing medium is 10% (V / V) FBS-containing DMEM, 3-isobutyl-1-methylxanthine (3-isobutyl-1-methylxanthine) is 0.5 mM, dexamethasone is 0.5 μM, What was prepared so that insulin (Insulin) might be 10 microgram / mL was used. Further, the differentiation maintenance medium used was a 10% (V / V) FBS-containing DMEM prepared such that insulin (Insulin) was 10 μg / mL.

(3) Measurement of AMPK activity FIG. 3 shows the results of measurement of adipocytes by the same method as the measurement of AMPK activity of the myoblasts of Example 2 above.

From FIG. 3, in the adipocytes, compared to each component alone group, an increase in AMPK activity was observed in the group in which all the components were combined, indicating that the composition of the present invention activates AMPK. .

From the above results, compared to the single group of processed banaba, processed maca and processed paloass, the combined group showed an increase in the number of myoblasts and an increase in AMPK activity of myoblasts and adipocytes. Because it was prominently seen, muscle mass was increased or maintained, such as metabolic improvement effect by promoting energy production by muscle, body tightening (slimming) effect, obesity prevention effect by preventing decline in basal metabolism due to muscle weakness, etc. Diet effect can be expected. Also, a beauty effect such as tightening the skin (preventing or improving wrinkles and sagging) by increasing or maintaining muscles can be expected.

Hereinafter, Formulation Examples 1 to 5 are illustrated as specific embodiments of the present invention, but the composition of the present invention is not limited only by the following Formulation Examples 1 to 5.

The raw materials were prepared according to the blending ratios described in the following formulation examples, and granules exhibiting the effects of the present invention were produced. In addition, the hydrated ethanol extract of the banaba leaf was used as the processed banaba, the hydrated ethanol extract of the maca bulb was used as the processed maca, and the hydrated ethanol extract of the above-ground part of Paloassul was used as the processed Paloastle.

The raw material was prepared according to the blending ratio described in the following formulation examples, and a tablet having the effects of the present invention was produced. In addition, the water-containing ethanol extract of the banaba leaf was used as the processed banaba, the dried powder of the maca bulb was used as the processed maca, and the dry powder of the above-ground part of the Paloastle was used as the processed taro.

The raw materials were prepared according to the blending ratios described in the following formulation examples, and soft capsules having the effects of the present invention were produced. In addition, the squeezed product of the banaba leaf was used as the banaba processed product, the squeezed product of the maca bulb part was used as the processed maca product, and the squeezed product of the above-ground part of the Palo Astle processed product was used.

Raw materials were prepared according to the blending ratios described in the following formulation examples, and hard capsules having the effects of the present invention were produced. In addition, the dried powder of banaba leaf was used as the processed banaba, the dried powder of the maca bulb was used as the processed maca, and the dried powder of the above-ground part of the Paloastle was used as the processed paloass.

The raw material was prepared according to the compounding ratio as described in the following prescription example, and the powdered drink which show | plays the effect of this invention was manufactured. In addition, the water extract of the banaba leaf was used as the processed banaba, the water extract of the maca bulb was used as the processed maca, and the hot water extract of the above-ground part of the Paloastle was used as the processed Paloastle.

Since the composition of one embodiment of the present invention can increase or maintain muscle mass and improve metabolism by promoting energy production by muscles, a person who wants a diet such as slimming or prevention of obesity, or a youthful It can be used to maintain the health of those who want to maintain or regain the appearance, train facial muscles such as wrinkles and sagging that cause it to appear older than the age, and activate skin turnover, etc. is there.

Claims (1)

A composition comprising a processed banaba product, a processed maca product, and a processed Paloisul product.