CN101731624B - Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process - Google Patents
Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process Download PDFInfo
- Publication number
- CN101731624B CN101731624B CN2009102634015A CN200910263401A CN101731624B CN 101731624 B CN101731624 B CN 101731624B CN 2009102634015 A CN2009102634015 A CN 2009102634015A CN 200910263401 A CN200910263401 A CN 200910263401A CN 101731624 B CN101731624 B CN 101731624B
- Authority
- CN
- China
- Prior art keywords
- silkworm moth
- male silkworm
- moth
- male
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 59
- 150000001875 compounds Chemical class 0.000 title claims abstract description 36
- 230000036039 immunity Effects 0.000 title abstract description 4
- 238000000034 method Methods 0.000 title description 14
- 230000008569 process Effects 0.000 title description 7
- 235000013305 food Nutrition 0.000 title description 2
- 230000001105 regulatory effect Effects 0.000 title description 2
- 230000003005 anti-senility effect Effects 0.000 title 1
- 239000003814 drug Substances 0.000 claims abstract description 31
- 241000005787 Cistanche Species 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 15
- 235000013402 health food Nutrition 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 10
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 15
- 244000241838 Lycium barbarum Species 0.000 claims description 14
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 9
- 229920001353 Dextrin Polymers 0.000 claims description 7
- 239000004375 Dextrin Substances 0.000 claims description 7
- 235000019425 dextrin Nutrition 0.000 claims description 7
- 230000001900 immune effect Effects 0.000 claims description 7
- 230000033228 biological regulation Effects 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000000796 flavoring agent Substances 0.000 claims description 4
- 235000019634 flavors Nutrition 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 2
- 239000007887 hard shell capsule Substances 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000007901 soft capsule Substances 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 abstract description 7
- 230000032683 aging Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000012467 final product Substances 0.000 abstract description 2
- 240000002624 Mespilus germanica Species 0.000 abstract 2
- 235000017784 Mespilus germanica Nutrition 0.000 abstract 2
- 235000000560 Mimusops elengi Nutrition 0.000 abstract 2
- 235000007837 Vangueria infausta Nutrition 0.000 abstract 2
- 230000007365 immunoregulation Effects 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 238000003809 water extraction Methods 0.000 abstract 1
- 239000002775 capsule Substances 0.000 description 34
- 241000699666 Mus <mouse, genus> Species 0.000 description 30
- 238000002474 experimental method Methods 0.000 description 21
- 229940079593 drug Drugs 0.000 description 18
- 210000005013 brain tissue Anatomy 0.000 description 13
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 12
- 241000208340 Araliaceae Species 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 9
- 235000003140 Panax quinquefolius Nutrition 0.000 description 9
- 235000008434 ginseng Nutrition 0.000 description 9
- 230000007812 deficiency Effects 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 206010003549 asthenia Diseases 0.000 description 7
- 230000002567 autonomic effect Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 229960000890 hydrocortisone Drugs 0.000 description 6
- 150000003254 radicals Chemical class 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 210000004698 lymphocyte Anatomy 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000857 drug effect Effects 0.000 description 4
- 150000002632 lipids Chemical group 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000002605 anti-dotal effect Effects 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000010255 intramuscular injection Methods 0.000 description 3
- 239000007927 intramuscular injection Substances 0.000 description 3
- 239000003726 plant lectin Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 235000014347 soups Nutrition 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 230000002929 anti-fatigue Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 229940000406 drug candidate Drugs 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 239000003777 experimental drug Substances 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 229940127022 high-dose drug Drugs 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 229940127021 low-dose drug Drugs 0.000 description 2
- 230000007721 medicinal effect Effects 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000255783 Bombycidae Species 0.000 description 1
- 244000056139 Brassica cretica Species 0.000 description 1
- 235000003351 Brassica cretica Nutrition 0.000 description 1
- 235000003343 Brassica rupestris Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical group CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 241000308150 Orobanchaceae Species 0.000 description 1
- 235000002791 Panax Nutrition 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003412 degenerative effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- JGEMYUOFGVHXKV-OWOJBTEDSA-N fumaraldehyde Chemical compound O=C\C=C\C=O JGEMYUOFGVHXKV-OWOJBTEDSA-N 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229930014550 juvenile hormone Natural products 0.000 description 1
- 239000002949 juvenile hormone Substances 0.000 description 1
- 150000003633 juvenile hormone derivatives Chemical class 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000010460 mustard Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- -1 oxygen radical Chemical class 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a compound male silk moth anti-aging immunoregulation health food, which is prepared by (1) drying male silk moth, squeezing and crushing by a screw oil press at constant temperature of 45 ℃ in a squeezing and crushing mode, sieving the squeezed product with a 12-mesh sieve, and collecting the sieved fine powder; (2) the medlar and the cistanche are mixed according to the ratio of 1: 1, and spray drying is carried out to form powder after water extraction; obtaining the extracted solid matter of the medlar and the cistanche; mixing to obtain the final product. The male silkworm moth compound patent medicine has the characteristics of good efficacy, simple and effective formula, smooth production process and the like, and is used for resisting aging and improving immunity of people.
Description
Technical field
The present invention relates to a kind of health food, is a kind of health food with anti-ageing, immunoloregulation function and preparation method thereof specifically.
Background technology
Along with progress of science and technology and expanding economy, people's rhythm of life is accelerated day by day, grows with each passing day from the pressure of each side such as work, life and society, and it is old and feeble to cause majority to be prone to, immunologic hypofunction.Aging be body with increasing age lentamente, the function reduction of progressive degenerative, disorderly general performance; It is the inexorable law of life process; It relates to many organs, and the decline of multisystem function and form and internal and external environment adaptive capacity and metabolic function is the biological phenomena of a complicacy.At present; The drug effect of prevention commonly used clinically and treatment aging is too single mostly; Can not improve the old and feeble sign of body, Chinese medicine then can more and more receive people's favor from angle enhances human body function, the raising resistance against diseases of comprehensively regulating because of it comprehensively.
Male silkworm moth contains several amino acids, vitamin and trace element, and is especially abundant with calcium, zinc, iron, cadmium content.Bioactivators such as a large amount of male sex hormones, juvenile hormone, brain hormone and moulting hormone in its body particularly are the good merchantable brands that integrates " integration of drinking and medicinal herbs " of dietotherapy, health, health care, help.
Some are arranged in the market is the functional health-care food of primary raw material with male silkworm moth; Like health foods such as male silkworm moths spirit, male silk moth capsules; Have antifatigue, improve the effect of immunity, but the shortcoming that these product formulation technologies fall behind: at first be that common drying and crushing is adopted in male silkworm moth powder process; Because the moth body and the moth wing are by local detachment, its patent medicine is renderd a service and is weakened; Secondly, the ubiquity prescription is complicated, and price is higher, contains a large amount of nourishing Chinese medicines, should not take for a long time, carries shortcomings such as inconvenience.
Summary of the invention
Above shortcoming in view of prior art the purpose of this invention is to provide the preparation method that a kind of master contains the compound male silkworm moth health food of male silkworm moth, makes it to overcome the deficiency of prior art, through the processing procedure of innovation, gives full play to the overall drug effect of male silkworm moth; And have that prescription is simple, technology is controlled, be easy to the characteristics of production and processing.
The present invention also aims to the compound male silkworm moth health food that provides a kind of master to contain male silkworm moth, be convenient for people to strengthen anti-ageing and raising immunity down in effective compound compatibility synergy.The objective of the invention is to realize like this:
The preparation method of the anti-ageing immunological regulation health food of a kind of compound male silkworm moth; Comprise following patent medicine step: male silk moth powder is after 30-50 ℃-oven dry low temperature drying in 15-25 hour; Squeeze (machine temperature is controlled within 45 ℃) one time through small-sized oil press, cross 12 mesh sieves, collect the fine powder after sieving.Matrimony vine and saline cistanche are extracted solid content and adopt following method to make: matrimony vine; Saline cistanche two flavor medicinal materials were by weight 1: 1 ratio compatibility; Amount of water is pressed 10 extraordinarily entry extractions of medicinal material gross weight, and twice, merge filtrating twice; Add the dry edible dextrin (weight adds by 1/2 of matrimony vine and saline cistanche medicinal material weight) of crossing 80 mesh sieves when heating is concentrated into relative density for 1.06-1.08, go into spray dryer powder process at 30-50 ℃.Merge male silkworm moth fine powder of above gained and matrimony vine saline cistanche and extract the solid content xeraphium, mixing promptly gets.
The anti-ageing immunological regulation health food of the compound male silkworm moth that adopts above method to make; Active principle is to get by following substances in parts by weight is evenly mixed: male silk moth powder (adult of the male Sichuan of Bombycidae silkworm Bombyx mori Linaeus) 20-80 part (weight portion; Down with), matrimony vine saline cistanche extraction solid content 15-60 part that matrimony vine (dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L.) and saline cistanche (succulent stem of the dry zone scale leaf of Orobanchaceae plant Inner Mongol saline cistanche Cistanche deserticolaY.C.Ma.) adopt as above technology to make.
The anti-ageing immunological regulation health food of compound male silkworm moth provided by the invention; Because male silkworm moth is adopted the special processing mode of pulverizing of pressing of squeezing; Moth body of male silkworm moth and moth alar part are pressed by the height extrusion; Male silkworm moth main component protein and lipid form effective fusion, compare with the drying and crushing of routine, play the uniqueness of activation drug effect and concoct effect.In addition, such greasy after the male silkworm moth that obtains of squeezing is pulverized unlike medicinal pulverizer can solve the technological requirement of the fluency that sieves.Through good prescription compatibility, make the medicinal effects synergy of three kinds of components, prescription is simple effectively, be easy to production and processing.
The specific embodiment:
Small-sized squeezer (the sx01 type Henan echo machinery adapted power 15-18kw of the Co., Ltd) discontinuity that the male silkworm moth of low temperature drying (in 15-25 hour drying of 30-50 ℃-oven dry) is used with peasant household's hot pickled mustard tube seed squeezes; Machine temperature is controlled at (generally 40-45 ℃ of scope, surpass this temperature and then shut down heat radiation, compressible is 5 kilograms in half an hour) about 45 ℃; Cross 12 mesh sieves; Fine powder after collection is sieved, rough segmentation stay once more and squeeze with oil press, and fine powder is for use.
Matrimony vine, saline cistanche two flavor medicinal materials were in 1: 1 ratio compatibility, and water is extraordinarily gone into by 10 of medicinal material total amount, and water extracts twice; Merge twice filtrating and add dextrin, dextrin (drying is crossed 80 mesh sieves) adds (respectively add 100g like matrimony vine, saline cistanche, then dextrin adds 50g) by 1/2 of single dose; In the time of 40 ℃, add and use the mixer mixing, filter, place electric furnace; It is to add spray dryer between the 1.06-1.08 that heating is concentrated into proportion, beats powder, gets powder; Pack is kept dry, and it is subsequent use to obtain matrimony vine saline cistanche extraction solid content.
By male silk moth powder: the matrimony vine saline cistanche is extracted solid content=20-80: 15-60 (weight portion) and evenly blends A, B, and the product of three kinds of different proportionings of C (is that heavy dose is 80: 60; Middle dosage is 40: 30; Low dose is 20: 15), make drug effect with positive thing and use contrast; Good effect is all arranged, wherein 40: 30 the bests (seeing following experimental result).
Medicinal effects is observed
(1) the antidotal effect of compound male silkworm moth capsule is observed
1 experiment material
1.1 animal used as test
Select 60 of healthy adult cleaning level Kunming white mouses for use, male, body weight 25 ± 5g, available from HuaXi college of pharmacy, SiChuan University Animal Experimental Study center, the conventional raising.
1.2 experiment medicine
(1) positive drug: Ginseng decoction.Genseng (the dry root of Araliaceae genseng Panax ginsengC.A.Mey.) is bought the big pharmacy of health shellfish in Chengdu.Preparation: take by weighing the 4.4g genseng in the 500ml large beaker, add suitable quantity of water and soak about 30min, place to decoct about 45min on the electric furnace, evaporation and concentration is promptly processed the Ginseng decoction that concentration is 0.022g/ml to 200ml, after the cooling, preserves with 4 ℃ of refrigerators, and is subsequent use.
(2) experimental drug: make the compound male silkworm moth capsule by preceding method, specification: 0.5g/ grain.
2 experimental techniques
2.1 the foundation of mice group and model of yang asthenia and medication
(1) animal divides into groups: 72 of healthy mices; Male; Be divided into 6 groups at random: dose drug group, compound male silkworm moth capsule low-dose drugs group in normal control group, model control group, positive drug control group, compound male silkworm moth capsule in high dose drug group, the compound male silkworm moth capsule, 12 every group.
(2) the mouse model of yang asthenia is set up: except that normal control group injection equal-volume physiological saline, all the other respectively organize all every intramuscular injection hydrocortisone (2mg/10ml) 0.2ml/d of mouse, and 7d causes model of yang asthenia continuously.In modeling, every of normal control group and model control group mouse are irritated stomach with physiological saline 0.6ml/d; Every of positive drug group mouse is irritated stomach with Ginseng decoction (0.022g/ml) 0.6ml/d; The high, medium and low dose groups mouse of compound male silkworm moth pharmaceutical capsules is irritated stomach, continuous 7 days with high, medium and low dosage soup (1.5g, 0.75g, 0.375g capsule 's content/d, this dosage is converted by people and animal-use drug dosage) respectively.
2.2 experimental technique
(1) mouse autonomic activities number of times determination experiment mouse autonomic activities number of times in 10min behind last administration 30min.
(2) superoxide dismutase (Superoxide Dismutase, SOD) and MDA (Maleic Dialdehyde, detection MDA).
This experiment adopts xanthine oxidase to measure the vigor of brain tissue SOD, and sulfo-barbital method (TBA method) is measured the content of brain tissue MDA.
2.3 statistical method
The completely random design is adopted in experiment.(x ± s) expression, the significance,statistical of difference is judged in the t check to experimental result between the employing group with mean ± standard deviation.
3 experimental results
3.1 symptom and sign are observed
The sign situation of each treated animal reacts good before the modeling, hair smoothing, and diet is normal, Quick off the mark.The most mouse of intramuscular injection hydrocortisone modeling the 5th day beginning model of yang asthenia group become thin, lassitude, movablely reduce, slow in reacting, the withered thin tarnish of chaeta, hogback also occurs and alarm symptoms such as hair, loose stool.Medication therapy groups and positive group compare with model group action more flexibly, big slightly, the hair of dietary amount is than moist, unusual behavior all has alleviation in various degree.
But deficiency of yang symptom is alleviated and improved to presentation of results compound male silkworm moth pharmaceutical capsules treatment group and Ginseng decoction's positive drug group antagonism hydrocortisone induced mice.
3.2 each group is to the influence of mouse autonomic activities number of times
Each group of table 1 is to the influence of experiment mice autonomic activities number of times (x ± s)
Annotate: represent P＜0.05 with compared with normal: ☆, ☆ ☆ representes P＜0.01; Compare with model control group: * representes P＜0.05; Compare with the positive drug group: # representes P＜0.05.
As test shown in the result, model group is compared the difference (P＜0.01) that highly significant is arranged with the normal control group, model of yang asthenia modeling success is described.Compound male silkworm moth capsule in high dose, middle dosage treatment group and positive drug group have been compared with model group than significant difference (P＜0.05); Explain that the compound male silkworm moth capsule is One's spirits are drooping to deficiency of yang mouse due to the hydrocortisone; Activity symptom slowly improves significantly; Significantly improve deficiency of yang mouse autonomic activities number of times (in experimentation; 1 of the dead mouse of normal control group, the dose groups mouse is each dead 2 in model control group, positive controls, medicine high dose group, the medicine, 3 of medicine low dose group dead mouses).
3.3 each group is to the influence of mouse brain tissue SOD and MDA
(1) each group is to the influence of mouse brain tissue SOD vigor, and experimental result is as shown in table 2:
Each group of table 2 is to the influence of experiment mice SOD (x ± s)
Annotate: with compared with normal: ☆ P＜0.05; Compare with model control group: * P＜0.05.
As test shown in the result, model group is compared brain tissue SOD vigor and is obviously reduced with the normal control group, significant difference (P＜0.05) is arranged, and model of yang asthenia modeling success is described.The compound male silkworm moth capsule is high, middle dosage treatment group is compared brain tissue SOD vigor with the positive drug group and obviously raise with model group; Have than significant difference (P＜0.05); Explain that the compound male silkworm moth capsule has tangible humidification to the brain tissue SOD vigor of deficiency of yang mouse due to the hydrocortisone; Thereby improve the ability that mouse is removed oxygen radical, delay senility.
(2) each organizes the influence of mouse brain being organized the MDA vigor, and experimental result is as shown in table 3:
Each group of table 3 is to the influence of experiment mice MDA (x ± s)
Annotate: with compared with normal: ☆ P＜0.05; Compare with model control group: * P＜0.05; * P＜0.01.
As test shown in the result, model group is compared brain tissue MDA content and is obviously raise with the normal control group, significant difference (P＜0.05) is arranged, and model of yang asthenia modeling success is described.The compound male silkworm moth capsule is high, middle dosage treatment group is compared brain tissue MDA content with the positive drug group and obviously reduced with model group; Highly significant difference (P＜0.01) is arranged; Explain that the compound male silkworm moth capsule has tangible reduction effect to the brain tissue MDA content of deficiency of yang mouse due to the hydrocortisone; Alleviate body inner lipid peroxidating degree, alleviate the cellular damage degree, thereby delay the senescence process of body tissue.
SOD is one of antioxidase important in the body, and its vigor and content have reflected the ability of body removing free radical.Research shows that with the growth at age, the ability that SOD removes free radical reduces gradually, so the height of SOD level is to weigh one of old and feeble important indicator.
Body aging, the Radical Metabolism meeting disequilibrium in the in-vivo tissue cell, body descends to the defence capability of free radical.The deposition of lipofuscin in cell tissue is the key character of organism aging process.The intermediate product that MDA forms as lipofuscin, its content can reflect the production and the peroxidating degree of body tissue cell to lipid of free radical in the body indirectly, so can be MDA as one of index of estimating aging.
This experiment is through the S&S of observation experiment mouse, and mensuration mouse swimming with a load attached to the body time, autonomic activities number of times, brain tissue SOD vigor, brain tissue MDA content are observed the compound male silkworm moth capsule to the antidotal effect of mouse.
Experimental result shows that the compound male silkworm moth capsule is similar with the positive drug Ginseng decoction, can obviously improve mouse deficiency of yang symptom; Prolong the mouse swimming with a load attached to the body time; Increase the autonomic activities number of times, improve the vigor of mouse brain tissue SOD, reduce the content that mouse brain is organized MDA.Show the ability that strengthens mouse muscle power, antifatigue, enhancing endurance, the antagonism deficiency of yang, removes free radical and reduction level of lipid peroxidation; Each dose groups and model group are more variant, and relevant with dosage, with the increase of dosage; These abilities strengthen, thereby reach certain antidotal purpose.
(2) the compound male silkworm moth capsule improves the effect observation of immunologic function
1 experiment material
1.1 animal used as test
Select 60 of healthy adult cleaning level Kunming white mouses for use, male, body weight 25 ± 5g, available from HuaXi college of pharmacy, SiChuan University Animal Experimental Study center, the conventional raising.
1.2 experiment medicine
(1) positive drug: Ginseng decoction.Genseng is bought the big pharmacy of health shellfish in Chengdu.
Ginseng decoction's preparation: take by weighing the 4.4g genseng in the 500ml large beaker, add suitable quantity of water and soak about 30min, place and decoct about 45min on the electric furnace; Evaporation and concentration is promptly processed the Ginseng decoction that concentration is 0.022g/ml, after the cooling to 200ml; Preserve with 4 ℃ of refrigerators, subsequent use.
(2) experimental drug: compound male silkworm moth capsule.The compound male silkworm moth capsule be the early stage preparation research during, the capsule medicine of processing.Specification: 0.5g/ grain.
The preparation of compound male silkworm moth capsule 's content soup: get 1.5g compound male silkworm moth capsule 's content porphyrize in mortar, add get behind an amount of distilled water appropriate amount of starch in mortar with capsule 's content porphyrize, mixing.Mixing liquid is gone in the small beaker, and adding distil water is heated to starch and becomes pasty state to 20ml on electric furnace, processes high dose and (contains the soup of 1.5g capsule 's content/20ml), cool off subsequent use.In dosage (contain 0.75g capsule 's content/20ml) and low dosage and (contain that the 0.375g capsule 's content/20ml) filling gastric juice is got final product by the high dose liquid diluting in use.
1.3 main agents
Phytohemagglutin phytolectin (phytohaemagglutinin, PHA): purchase Rui Sheng Science and Technology Ltd., specification 5mg/ bottle in Sichuan.
1.4 key instrument
Biomicroscope (BH one 2 types, OLYMPUSCo, LTO product)
Electro-heating standing-temperature cultivator (HHBll420 one S one 11 types, Shanghai make a leapleap forward medical apparatus and instruments factory's product)
Light microscope (XSP one 16A type, optical instrument factory, south of the River product)
Electronic balance (1004 types, Shanghai Precision Scientific Apparatus Co., Ltd's product)
2 experimental techniques
2.1 animal divides into groups
72 of healthy mices; Male; Be divided into 6 groups at random: dose drug group, compound male silkworm moth capsule low-dose drugs group in normal control group, model control group, positive drug control group, compound male silkworm moth capsule in high dose drug group, the compound male silkworm moth capsule, 12 every group.
2.2 experimental technique
(1) phytohemagglutin phytolectin (PHA) the lymphocyte transformation experiment of inducing
Experimental technique: mice group, modeling, administration add intramuscular injection 10mg/kg phytohemagglutin phytolectin in the 1st, 2,3 days every mouse of administration as stated.Administration every day 1 time, successive administration 7d.The 8th day docking blood sampling smear, Wright's staining, get under the oily mirror slide head, in, trail a machine counting lymphocyte, calculate conversion ratio.
200 of lymphocyte transformation rate %=(lymphocyte number of conversion * 100%)/TLC
2.3 statistical method
The completely random design is adopted in experiment.(x ± s) expression, the significance,statistical of difference is judged in the t check to experimental result between the employing group with mean ± standard deviation.
3 results
Experimental result is as shown in table 4:
Each group of table 4 is to the influence of experiment mice lymphocyte transformation rate (x ± s)
Annotate: with compared with normal: ☆ P＜0.001; Compare with model group: * * P＜0.001.
Experimental result shows, compares with the normal control group, and model group PBLC conversion ratio significantly reduces (P＜0.001), explains that the immunocompromised model prepares successfully.With the model group ratio, high, middle dosage compound male silkworm moth pharmaceutical capsules treatment group and Ginseng decoction's positive drug group all can make the PBLC conversion ratio raise, and there were significant differences (P＜0.001) for dose groups and positive drug group and model group in its Chinese traditional medicine.
The auxiliary process experiment shows saline cistanche two flavor medicinal materials by 1: 1 compatibility, and water extracts twice, and water inventory is extraordinarily gone into by 10 of medicinal material total amount, merges filtrating twice; Be controlled at 40 ℃ and added the dry dextrin stirring and evenly mixing of 80 mesh sieves by 1/2 of simple weight; It is that to carry out spraying drying powder-forming between the 1.06-1.08 be to have the working condition of technology fluency and cost control preferably that heat is concentrated into proportion.
Basic scheme based on the present invention proposes can have multiple variation when actual production is made, such as, edible dextrin can use other edible adjuvants of this ratio value (being the relative weight ratio of active material) to add that the solid shape of health care replaces.Health food can be processed pill, tablet, soft capsule, hard shell capsules, granule, oral liquid etc. according to actual needs.
The processing processing procedure fluency of male silkworm moth: see from the angle of technology,, be unfavorable for and other medicinal material batchings and filling capsule because male silkworm moth main component is protein and lipid; Or utilize the grease absorption of the male silkworm moth after oil-Absorbing Sheets will be pulverized clean, though can obtain being beneficial to particle very time-consuming and the oil-Absorbing Sheets of loading capsule at last, render a service and improve processing cost but can influence patent medicine.Such greasy after the male silkworm moth that the present invention squeezing obtains is pulverized unlike medicinal pulverizer; Can satisfy the demand that smoothness is sieved; Particle after sieving can with other batch mixes evenly and satisfy the condition of loading capsule, and the meal after sieving can carry out twice pressing.
Claims (2)
1. the preparation method of the anti-ageing immunological regulation health food of compound male silkworm moth comprises following patent medicine step:
(1) male silkworm moth is after 15-25 hour drying of 30-50 ℃ of oven dry, and through adopting the mode of pulverizing of pressing of squeezing, thermostatic control is at 45 ℃, adopts screw oil expeller to squeeze to press to pulverize, and extract is crossed 12 mesh sieves, collects the fine powder after sieving;
(2 matrimony vines, saline cistanche two flavor medicinal materials were by 1: 1 compatibility, and water extracts twice, and water inventory is extraordinarily gone into by 10 of medicinal material total amount, merges filtrating twice; Be controlled at 40 ℃ and added the dry dextrin stirring and evenly mixing of 80 mesh sieves by 1/2 of simple weight; It is to carry out spraying drying powder-forming between the 1.06-1.08 that heat is concentrated into proportion; Obtain the matrimony vine saline cistanche and extract solid content;
(3) above two step gains evenly mix in proportion, and weight ratio is: male silk moth powder 40 weight portions, the matrimony vine saline cistanche is extracted solid content 30 weight portions, obtains product.
2. according to the anti-ageing immunological regulation health food of the said compound male silkworm moth of claim 1, it is characterized in that: said health food can be made into pill, tablet, soft capsule, hard shell capsules, granule, oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102634015A CN101731624B (en) | 2009-12-15 | 2009-12-15 | Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102634015A CN101731624B (en) | 2009-12-15 | 2009-12-15 | Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101731624A CN101731624A (en) | 2010-06-16 |
| CN101731624B true CN101731624B (en) | 2012-05-09 |
Family
ID=42456037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102634015A Expired - Fee Related CN101731624B (en) | 2009-12-15 | 2009-12-15 | Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101731624B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126775A (en) * | 2014-06-23 | 2014-11-05 | 王奎 | Duck meat nourishing glutinous fragrant rice and preparation method thereof |
| CN108124964A (en) * | 2017-12-11 | 2018-06-08 | 长春三真实业有限公司 | A kind of establishing-Yang male silk moth milk tablet |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403564A (en) * | 2002-07-27 | 2003-03-19 | 战青芳 | Nourishing male silk moth wine and its making process |
-
2009
- 2009-12-15 CN CN2009102634015A patent/CN101731624B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1403564A (en) * | 2002-07-27 | 2003-03-19 | 战青芳 | Nourishing male silk moth wine and its making process |
Non-Patent Citations (2)
| Title |
|---|
| 刘绍等.食品免疫与免疫食品新进展.《实用预防医学》.2004,第11卷(第3期),第635－636页. * |
| 周宝宽.抗疲劳防治亚健康中药整理与研究.《中华中医药学刊》.2008,第26卷(第1期),第193-195页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101731624A (en) | 2010-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103099089B (en) | Health-care product for reducing blood sugar and blood pressure, relaxing bowels, losing weight, resisting fatigue and improving sexual function and production technology thereof | |
| CN102907604B (en) | Health-care product having effects of reducing blood sugar and pressure, losing weight, relaxing bowels, maintaining beauty and resisting fatigue and production process thereof | |
| CN102406860B (en) | Composition for preventing and treating diabetes, and preparation method and use thereof | |
| CN102919836B (en) | Health-care product capable of reducing blood glucose, releasing pressure, reducing lipid, relaxing bowel, beautifying, losing weight and resisting fatigue and production process thereof | |
| CN101822749B (en) | Medicinal preparation for curing hypertension and hyperlipaemia and preparation method thereof | |
| US20190328817A1 (en) | Traditional chinese medicine healthcare preparation for fat elimination and weight reduction | |
| CN104095231B (en) | A kind of health products containing agate coffee, the stem of noble dendrobium, American Ginseng and preparation method thereof | |
| CN103417837A (en) | Medical and edible Chinese wolfberry health maintenance cream and preparing method thereof | |
| CN103734421A (en) | Liver-benefiting kidney-tonifying stomach-invigorating tea and preparation method thereof | |
| CN105532998A (en) | Health-care tea containing cordyceps militaris and preparation method thereof | |
| CN102652801A (en) | Chinese herbal medicine tea bag for lowering blood sugar and preventing diabetes mellitus complications | |
| CN102178217B (en) | Konjac-flour-containing mixture and preparation method thereof | |
| CN102551065A (en) | Blood sugar reducing food series | |
| CN104432063B (en) | Composition for alleviating athletic fatigue and preparation method thereof | |
| CN104187681B (en) | A kind of reducing blood sugar and blood fat health food and preparation method thereof | |
| CN102579836A (en) | Traditional Chinese medical dendrobium huoshanense composition and preparation method thereof | |
| CN101731624B (en) | Compound health-care food for anti-senility and regulating immunity of male silkworm moth and its preparing process | |
| CN102389114B (en) | Health food with the function of physical fatigue alleviation and preparation method thereof | |
| KR20160141027A (en) | Phamaceutical composition or healthy food comprising water extracts from Pleurotus eryngii var. ferulea (Pf.). for treating or preventing metabolic disorder | |
| CN108066384A (en) | A kind of Chinese medicine preparation with strengthen immunity and preparation method thereof | |
| CN105101817B (en) | Edible composition and preparation method thereof and the food comprising said composition | |
| CN102499366B (en) | Health-care food for adjusting blood fat and preventing fatigue | |
| CN105495603A (en) | Preparation method for radix ginseng rubra-fructus crataegi cell-wall breaking superfine powder | |
| CN101120750A (en) | Method for preparing ginkgo bee product | |
| JP6273440B2 (en) | GLP-1 production promoter, DPPIV inhibitor and glucose absorption inhibitor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120509 Termination date: 20141215 |
|
| EXPY | Termination of patent right or utility model |