JP2014135949A - Culture medium information registration system, culture medium image analysis device, program, and health management system - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a culture medium information registration system and so forth which facilitates confirmation work of state confirmation of each culture medium so that workload of an administrator can be alleviated.SOLUTION: A health management system S, when a predetermined time passes after culture starts, performs an image analysis processing analyzing a culture medium image imaged from a film type culture medium 60, sorts out a colony from a non-object (non-colony) such as dust among the colony candidates detected based on feature quantities of pixels constituting the culture medium image by using a likelihood indicating plausibility of the colony as a uniform reference, and provides an administrator with image data by which the administrator can notice a colony having possibility of false detection or a non-colony.

Description

  The present invention relates to a food hygiene management system and the like.

  In recent years, due to the growth of fast food and other food service industries and the increase in easy processed foods, the opportunity to eat food prepared by others has increased remarkably. On the other hand, when foods such as processed foods and processed foods are mixed with dangerous substances such as poisons or microorganisms, it directly affects the human body. When handling processed foods, not only the safety of raw materials but also the production There is a need for food safety that strictly controls the hygiene of the process and covers the entire production and processing of food.

  In particular, food poisoning incidents that have occurred in various places are the starting point, and it is a proposition to further improve the safety of foods. In addition to complying with the Food Sanitation Law, “HACCP (Hazard Analysis Critical Control) An increasing number of companies have introduced “Point System” and “FSSC (Food Safety System Certification 22000)”.

  As a system for detecting microorganisms mixed in such processed foods, a film-type medium is used, a specimen cultured in the film-type medium is imaged, and image processing is performed on the image of the imaged medium. Thus, there is known a method for detecting colonies that are a mass of bacteria generated in a specimen and counting them (for example, Patent Document 1).

  In particular, in such a system, it is important to accurately count the number of colonies of different sizes and shapes from the viewpoint of hygiene management indicators. A colony detection system is known in which the shape of each colony is specified by using a plurality of thresholds with respect to the feature amount of each pixel constituting and the number of colonies is detected (for example, Patent Document 2).

JP 2011-2112013 A JP 2012-75409 A

  However, in the system for detecting colonies such as Patent Document 2, colonies are automatically detected from an image of a culture medium, but there are many false detections. Therefore, in such a case, when an erroneous detection is confirmed by the administrator, the administrator manually cancels the erroneously detected colony or registers an undetected colony, which complicates complicated operations. It has become. In particular, such a colony system is prone to erroneous detection depending on the bacterial species or imaging environment, and it is difficult to improve the workability of the administrator's confirmation work.

  The present invention has been made to solve the above-described problems, and its purpose is to facilitate the confirmation work for confirming the status of each medium, and to reduce the work load of the administrator. It is to provide a medium information registration system and the like.

  In order to solve the above-described problem, a culture medium information registration system and the like according to the present invention includes an acquisition unit that acquires culture medium data obtained by imaging a culture medium as culture medium image data, and the acquired culture medium image data. Based on the feature amount relating to the color of each pixel constituting the medium image of the above, a detection means for detecting a colony candidate as a colony candidate and the feature amount relating to the color of the pixel for each detected colony candidate Calculation means for calculating the likelihood of each colony candidate based on the characteristics of the colony candidate; and selection means for selecting the detected colony candidate into the colony and non-colony based on the calculated likelihood; , At least one of the selected colonies and the selected non-colonies based on the calculated likelihood, two or more A discriminating means for discriminating the type and image data for imaging the discriminating result indicating the discriminated result on the medium image in a predetermined display mode are generated, and the generated image data is browsed by an administrator. Providing means for providing to the display means, and registration means for registering information on the medium including information on the colonies in the database as medium information based on the determination result.

  Since the culture medium information registration system and the like according to the present invention can discriminate colonies or non-colonys into two or more types based on the likelihood indicating the degree of likelihood of colonies, the characteristics of non-colony are determined according to a uniform standard. It is possible to discriminate between colonies that are likely to be erroneously detected and non-colony types, such as colonies that are close to and non-colony that have characteristics close to those of the colonies.

  Therefore, the culture medium information registration system according to the present invention facilitates the confirmation work for confirming the status of each culture medium by displaying the discrimination result between the colony and the non-colony for the administrator. The work burden can be reduced, and as a result, colonies can be accurately identified and medium information including them can be registered accurately.

It is a lineblock diagram showing the composition of one embodiment of the hygiene management system concerning the present invention. It is an example of the film type culture medium used for the sanitary management system of one Embodiment. It is an example of the work instruction used for the sanitary management system of one embodiment. It is a block diagram which shows the structure of the portable communication terminal device used for the sanitary management system of one Embodiment. It is a block diagram which shows the structure of the server apparatus used for the sanitary management system of one Embodiment. It is a figure for demonstrating the likelihood calculation process performed in the server apparatus of one Embodiment. It is a figure for demonstrating the colony selection process performed in the server apparatus of one Embodiment. It is a figure for demonstrating the false detection colony discrimination | determination process performed in the server apparatus of one Embodiment. It is a figure for demonstrating the colony correction process performed in the server apparatus of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the portable communication terminal device of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the server apparatus of one Embodiment. It is the flowchart (the 1) which shows operation | movement of the colony detection determination process including the analysis result acquisition display process performed by the administrator terminal device in the server apparatus of one Embodiment. In the server apparatus of one Embodiment, it is a flowchart (the 2) which shows operation | movement of the colony detection determination process containing the analysis result acquisition display process performed by the administrator terminal device.

  Hereinafter, embodiments according to the present invention will be described with reference to the drawings. In addition, the following embodiment is a medium information registration system, program, and the like according to the present invention for a hygiene management system using a film-type medium in a food production line or an inspection line (hereinafter referred to as “work line”). It is embodiment at the time of applying a medium image analysis device and a hygiene management system. However, the present invention is not limited to the following embodiments within the scope including the technical idea.

[1] Hygiene management system First, the hygiene management system S of this embodiment will be described with reference to FIG. In addition, FIG. 1 is a block diagram which shows the structure of the sanitary management system S of this embodiment.

  The sanitary management system S of the present embodiment uses a film-type culture medium (that is, a film-type culture medium) and performs data management on information on the culture medium (hereinafter referred to as “medium information”), thereby producing food. Or it is a system for inspecting the sanitary condition on inspection.

  In addition, the sanitary management system S of the present embodiment extracts a food during or after manufacture as a specimen in a work line 80 having a manufacturing process for manufacturing a processed or processed food or an inspection process for inspecting the food. In addition, this is a system for managing data on the culture state when a pre-specified bacterium such as a general bacterium or E. coli or the like occurring in a specimen in the film-type medium 60 is cultured.

  Then, the hygiene management system S generates information on the work in the manufacture or inspection of food (hereinafter referred to as “work information”) and the extracted sample in order to manage and determine the sanitary state of the food on the data. The culture medium information of the film-type culture medium 60 acquired by the portable communication terminal device 10 before and after culturing the fungus (colony) to be registered can be associated and registered in the server device 40 (specifically, the database 400). It has a possible configuration.

In particular, the hygiene management system S of the present embodiment, after a predetermined time has elapsed since the start of culture,
(1) A process (hereinafter referred to as “image analysis process”) of analyzing a medium image in which the film-type medium 60 is imaged is executed.
(2) Non-objects such as colonies and dust using the likelihood indicating the likelihood of colonies as a uniform reference from among colony candidates detected based on the feature values of pixels constituting the medium image ( (Hereinafter referred to as “non-colony”)
(3) It has a configuration in which the administrator is provided with image data for causing the administrator to pay attention to a colony or a non-colony with a possibility of erroneous detection.

  And the sanitary management system S of this embodiment is the number of colonies of colonies detected and selected based on the medium image while accepting corrections to colony candidates from the administrator or correction instructions to non-colony colonies. Is stored in the database 400.

  In order to realize such a configuration, the sanitary management system S of the present embodiment, as shown in FIG. 1, includes a plurality of portable communication terminal devices 10 used when registering culture medium information regarding each film-type culture medium 60. In addition, the administrator terminal device 20 that manages the sanitary management system S, the network 30, and the database 400, and in conjunction with the portable communication terminal device 10 or the administrator terminal device 20, registration management of medium information is performed. And a server device 40 that executes various types of processing.

  The work information of this embodiment includes, in addition to various types of information related to the manufacturing process or the inspection process, information regarding the culture of the food extracted from the manufacturing process or the inspection process as a specimen and the colonies generated in the specimen. In addition, it is registered in the database 400 using unique identification information (hereinafter referred to as “work ID”).

Specifically, each work information includes
(1) Work ID,
(2) Identification information (hereinafter referred to as “line ID”) of the work line 80 from which the sample is to be extracted or, if the work line 80 has a plurality of processes, the line ID and process identification information (hereinafter referred to as “line ID”). , Referred to as “process ID”) 71b;
(3) Product name of food as a sample, identification information of lot (hereinafter referred to as “lot ID”), lot name,
(4) Type of bacteria detected from specimens such as general bacteria or E. coli (hereinafter referred to as “test type”);
(5) Sample culture conditions such as dilution factor, type of diluent, culture temperature, culture humidity, and culture time,
(6) Specimen culture start time;
(7) a time after a predetermined time has elapsed since the start of culture of the specimen (hereinafter also referred to as “culture test time”);
(8) Sample incubator location such as incubator number and number of stages in incubator,
(9) Work date and time indicating the work start time and end time;
(10) Worker ID (employee ID) and worker name;
Is included. Each piece of work information is registered in the database 400 for each type and for each work.

  The portable communication terminal device 10 is, for example, a digital camera having a communication function, a tablet-type information terminal device, a smartphone or a portable telephone, or the like, or an imaging function such as a still image or a recording function (hereinafter simply referred to as “camera function”). And a communication terminal device portable by an operator.

  In particular, the portable communication terminal device 10 generates an image data (hereinafter referred to as “medium image data”) by imaging the film-type culture medium 60 by the camera function, and also images the work instruction sheet 70 and displays the instruction sheet. It has a configuration capable of generating an image.

  Then, the portable communication terminal device 10 acquires the work ID 71 and the culture medium ID 66 from the image data of the instruction manual image (hereinafter referred to as “instruction data image data”) and the culture medium image data of the film type culture medium 60, The acquired work ID 71 and medium ID 66, medium image data, and metadata of the medium image data can be registered in the database 400 of the server device 40 as medium information.

  On the other hand, when the portable communication terminal device 10 transmits the culture medium information to the server device 40, BLUETOOTH (registered trademark), wireless LAN (WLAN: Wireless Local Area Network), or wireless PAN (WPAN: Wireless Personal Area Network). The data is transmitted to the server device 40 directly or via the access point 50 using a communication standard for short-range wireless communication or the like, or is transmitted to the server device 40 using a public telephone line network via a mobile base station (not shown). It has a configuration.

  The portable communication terminal device 10 has a browser function constructed by a markup language such as XML (extensible Markup Language), and executes an operation input instruction and an operation confirmation by the operator using the browser function. The culture medium information is transmitted to the server device 40 via the browser function.

  The administrator terminal device 20 is an information communication terminal device such as a tablet information terminal device, a smartphone, a personal computer, or a workstation. The administrator terminal device 20 manages the administrator identification information (hereinafter referred to as “manager ID”), the administrator password, and the worker identification information (hereinafter referred to as “worker ID”). It functions as a control device capable of performing management of access authority to the server device 40, management of the terminal ID of the portable communication terminal device 10, correction of registered medium information, and other management.

  Similarly to the portable communication terminal device 10, the administrator terminal device 20 has a browser function constructed by a markup language, and uses the browser function to exchange data with the server device 40 and to generate a report. It has a configuration capable of executing browsing and the like.

In particular, the administrator terminal device 20
(1) A process of acquiring the analysis result of the medium image by the image analysis process from the server device 40 based on the administrator's instruction and displaying it together with the medium image (hereinafter referred to as “analysis result acquisition display process”)
(2) A process of coordinating with the server device 40 to correct a colony displayed together with the medium image according to the obtained analysis result to a non-colony, and to correct a colony candidate (that is, a non-colony) that is not identified as a colony to a colony ( Hereinafter referred to as “colony correction processing”),
It has the structure which performs.

  Note that the administrator terminal device 20 determines the culture medium information when each operation is interrupted due to a failure of a machine that performs the process or the like in the same lot, or when an error in registration of culture medium information occurs in the operator. It has a configuration that can correct other information.

  The network 30 includes, for example, a public telephone line network (hereinafter referred to as “long-distance communication network”) including a mobile phone network, an IP (Internet Protocol) network such as a short-distance wireless network, or both of which are interconnected. Has been. However, the configuration of the network 30 is not limited to this.

  The server device 40 is a server device used in conjunction with the portable communication terminal device 10 or the administrator terminal device 20 to execute each data process for hygiene management of the lot and work line 80.

  Then, the server device 40 is linked with the portable communication terminal device 10, registers the culture medium information in each film-type medium 60 before the start of culture (hereinafter referred to as “initial registration”), and starts the culture in the database 400. A medium formed by media information transmitted from the portable communication terminal device 10 and media image data included in the acquired media information while performing subsequent registration (hereinafter referred to as “post-culture registration”) It has a configuration for detecting colonies generated and cultured in a specimen from an image.

Specifically, when the server device 40 acquires medium image data having a medium image,
(1) Based on the feature amount related to the color of each pixel constituting the medium image, a colony candidate that is a lump of bacteria is detected as a colony candidate,
(2) For each detected colony candidate, calculate the likelihood of each colony candidate based on the characteristics of the colony candidate determined by each pixel constituting the colony candidate image,
(3) Based on the calculated likelihood, the detected colony candidate is sorted into a colony and a non-colony,
(4) At least one of the selected colony and the selected non-colony is discriminated into two or more types based on the calculated likelihood.
(5) The administrator terminal device 20 for generating image data for imaging the determination result indicating the determined result on the culture medium image in a predetermined display mode, and causing the administrator to browse the generated image data. Offer to,
(6) The medium information is registered in the database 400 based on the determination result.

In particular, the server device 40 performs the process (4).
(4A) The calculated likelihood of each colony is compared with a predetermined first threshold, and the first colony and the colony candidate selected as a non-colony from the first colony are justified. A first discrimination process for discriminating a second colony having a close degree (ie, an overdetection correction candidate), and (4B) comparing the calculated likelihood of each non-colony with a predetermined second threshold, Discrimination process for discriminating a first non-colony from the first non-colony and a second non-colony (ie, an undetected correction candidate) having a close likelihood to a colony candidate selected as a colony from the first non-colony ,
It has the structure which performs.

In addition, the server device 40 performs the process (5).
(5A) Image data with different display modes of the first colony and the second colony,
(5B) Image data with different display modes of the first non-colony and the second non-colony, and
(5C) It has the structure which produces | generates the image data of at least any one of the image data from which the display mode of a colony and a non-colony differs.

  Then, the server device 40 is linked with the administrator terminal device 20 and is used when selecting a colony and a non-colony based on an instruction from the administrator, or when determining a type of a colony or a non-colony. Or resetting the parameters. In particular, the parameters include the above-described selection threshold (hereinafter also referred to as “colony selection threshold”), the first threshold (hereinafter also referred to as “colony erroneous detection confirmation threshold”), and the second threshold. (Hereinafter also referred to as “non-colony erroneous detection threshold”).

  Furthermore, the server device 40 has a configuration that accepts correction of the determination result based on an instruction from the administrator in conjunction with the administrator terminal device 20.

  Note that the server device 40 may execute only one of the first determination process or the second determination process, and based on the calculated likelihoods, the rank in at least one of the second colony and the second non-colony is determined. The display mode may be changed based on the ranking.

  With such a configuration, in the sanitary management system S of the present embodiment, the likelihood that indicates the likelihood of colony is uniformly used from among the colony candidates detected based on the feature amount of the pixels constituting the medium image. Colonies or non-colonies can be selected according to the standard, so that colonies and non-colonies that may be erroneously detected (that is, colonies with a likelihood close to a non-colony and non-colony with a likelihood close to a colony) ), And the display mode of the colony or non-colony having a high possibility of erroneous detection can be changed to other colonies or non-colony such as highlighting.

  Therefore, in the sanitary management system S of the present embodiment, unlike the other colonies or non-colony, the administrator can easily watch the colony or non-colony that is likely to be erroneously detected. In addition, the confirmation work for confirming the status of each medium can be facilitated, and the workload of the administrator can be reduced. As a result, the colonies are accurately identified, and the medium information including them is accurately registered. Be able to.

  In the sanitary management system S of the present embodiment, it is possible to save resources as compared with the case where various data analysis and provision are performed using physical resources such as paper media, and the culture medium. By registering media information on the database in the database, registration errors can be significantly reduced and difficult media management can be facilitated, so resources can be saved without wastefully consuming media. Yes.

[2] Film Type Medium Next, the film type medium 60 of the present embodiment will be described with reference to FIG. FIG. 2 is an example of a film-type medium 60 used in this embodiment.

  The film-type medium 60 used in the present embodiment is a medium for culturing bacteria generated in food as a specimen detected from each step of the work line 80 using a film or sheet-like dry medium. For example, the film-type culture medium 60 is used as a culture medium for cultivating general live bacteria, coliform bacteria, and Staphylococcus aureus. In addition, you may use as a culture medium which culture | cultivates various bacteria, substances, or microorganisms, such as mold | fungi, yeast, a Listeria bacterium, a microorganism for water quality, lactic acid bacteria, and a protein.

  The film-type medium 60 includes, for example, a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG. (Hereinafter referred to as “circular frame”) 62, a culture layer 63 for culturing bacteria provided in the frame, a cover sheet 64 covering the culture layer 63, and a base sheet 61. Medium ID66.

  For example, the culture medium ID 66 is formed of a barcode such as a two-dimensional barcode or alphanumeric characters, and is imaged together when the film-type culture medium 60 is imaged, such as a barcode recognition function or an OCR (Optical Character Recognition) function. It is acquired in the portable communication terminal device 10 or the server device 40 using the image analysis function.

  The base material sheet 61 is not particularly limited as long as it is a film-like or sheet-like base material, and for example, a plastic film or paper can be used. Preferred examples of plastic films include resin films such as polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polymethacrylate, polymethyl methacrylate, polymethyl acrylate, polyester, and polycarbonate. Preferred examples include polyethylene terephthalate and polypropylene synthetic paper. Note that the polypropylene-based synthetic paper is a film synthetic paper whose main raw material is polypropylene.

  The film-type culture medium 60 has an IC tag in which a culture medium ID 66 is stored. The short-range wireless communication interface 140 (for example, an IC tag reader) included in the portable communication terminal apparatus 10 causes the medium to be connected to the portable communication terminal apparatus 10. ID66 may be acquired.

[3] Work instruction sheet Next, the work instruction sheet 70 of this embodiment will be described with reference to FIG. FIG. 3 is an example of a work instruction sheet 70 used in the present embodiment.

  The work instruction sheet 70 of the present embodiment includes, for each food group manufactured or inspected under the same conditions, that is, for each lot, work names and contents of one or more manufacturing processes or inspection processes, and work lines 80 to be used. It is an instruction document that describes the name of the food (processed food) to be manufactured or inspected, the quantity, and other information.

  In addition, the work instruction sheet 70 of the present embodiment has an instruction sheet ID 71a formed by printing or other methods on the upper left, and a plurality of process IDs 71b formed by printing or other methods on a predetermined area. . The instruction sheet ID 71a and each process ID 71b are used as the work ID 71, and are formed of, for example, a barcode such as a two-dimensional barcode or alphanumeric characters. The instruction ID 71a and each process ID 71b are imaged in the same manner as the film-type medium 60 when the registration of the film-type medium 60 is executed, and analyzed by the portable communication terminal device 10 or the server device 40. Be recognized.

  The work instruction sheet 70 has an IC tag in which the work ID 71 of the instruction sheet ID 71a or the process ID 71b is stored, like the film type culture medium 60, and has an interface (short distance) that the portable communication terminal device 10 such as a tag reader has. The portable communication terminal device 10 may acquire the work ID 71 by the wireless communication interface 140). In the following description, unless otherwise specified, the work ID 71 indicates an instruction ID 71a or a process ID 71b.

[4] Portable Communication Terminal Device Next, the configuration of the portable communication terminal device 10 of the present embodiment will be described using FIG. FIG. 4 is a block diagram showing the configuration of the portable communication terminal device 10 of the present embodiment.

  The portable communication terminal device 10 of the present embodiment has a data storage unit 100 having a memory function used when various programs are executed, an imaging function, image data of the film-type medium 60, and other image data. In addition to the image data generation unit 110 that generates the media information, the media information is registered in the server device 40 after the initial registration and the post-cultivation registration immediately after the culture in conjunction with the server device 40 (hereinafter referred to as “medium information registration processing”). And an application control unit 120 that executes processing.

  The portable communication terminal device 10 includes a network communication unit 130 that communicates with the server device 40 and other communication devices, a short-range wireless communication interface 140 that exchanges data with an IC tag and other communication interfaces, Controls the current position detection unit 150 that detects the current position, the display unit 160, the display control unit 161 that controls the display unit 160, the operation unit 170 for inputting user operations, the timer 180, and the entire apparatus. A portable terminal management control unit 190.

  And the portable communication terminal device 10 has various necessary members such as a microphone, a speaker, and an e-mail transmission / reception function when having a mail function such as a telephone function and e-mail. Further, the above-described units are connected to each other by a bus 11 to exchange data.

  The data storage unit 100 includes an application storage unit 101 that stores various application programs, an image data storage unit 102 that stores image data captured and generated by the image data generation unit 110, and a portable communication terminal device 10 and management / control programs, and a ROM / RAM 103 that is used as a work area during execution of each program and stores data used in each process executed by the portable communication terminal device 10. doing.

  In particular, the application storage unit 101 includes an application program (hereinafter referred to as “application”) that is executed by the application control unit 120 in conjunction with the image data generation unit 110, the operation unit 170, the display control unit 161, and the image data storage unit 102. Is recorded). The application storage unit 101 also stores a browser program for realizing the above browsing function.

  In the image data storage unit 102, the imaged medium image data and instruction sheet image data, the image ID for managing each image data, various metadata corresponding to each image data such as the imaging time, and the work ID 71 The medium ID 66 and various flag information are stored in association with each other. The image ID is arbitrary identification information that is appropriately given in each portable communication terminal device 10.

  The image data generation unit 110 includes an optical system, a CCDI sensor (Charge Coupled Device Image Sensor) that converts an optical image input from the optical system into an electrical signal, and image data based on the electrical signal generated by the CCDI sensor. And a generating unit that generates

  In particular, when the work instruction sheet 70 is imaged, the image data generation unit 110 converts the imaged work instruction sheet 70 into an image and generates instruction sheet image data. The image data generation unit 110 images the film-type medium 60 that has been imaged when the bacteria generated in the specimen are cultured or the film-type medium 60 that has been cultured is imaged. 60 pieces of medium image data are generated as medium image data.

  The application control part 120 implement | achieves culture-medium information registration process with a predetermined application. In particular, the application control unit 120 executes the network communication unit 130 and the display control unit 161 while executing various control programs for controlling each unit of the portable communication terminal device 10 using the culture medium registration application stored in the application storage unit 101. In addition, various processes are executed in conjunction with or controlled by the operation unit 170.

  Specifically, the application control unit 120 executes the medium registration application recorded in the application storage unit 101, acquires the medium image data of the film-type medium 60 imaged by controlling the image data generation unit 110, The image data is stored in the image data storage unit 102.

  Then, the application control unit 120 acquires the medium ID 66 from the imaged medium image data, and together with the acquired medium ID 66, the image data storage unit displays the medium image data, the metadata of the medium image data, and various flag information. 102. Note that the application control unit 120 may execute barcode recognition or OCR recognition in conjunction with another communication device or the database 400 via a communication line.

  In addition, the application control unit 120 performs initial registration for registering the culture medium information in the server device 40 at the start of the culture of the specimen after imaging of the culture medium image data or at a predetermined timing, and registers the culture medium information after the start of the culture of the specimen. Perform registration after incubation. In particular, the application control unit 120 identifies whether the registration is initial registration or post-cultivation registration based on the operator's instruction, and includes information (flag information) indicating either registration in the culture medium information in the server device 40. Send.

  The application control unit 120 acquires the current time from the timer 180 when the film-type medium 60 is imaged, and the image data storage unit as the metadata of the medium image data together with a predetermined image ID using the current time as the imaging time. 102. When the medium image data is transmitted to the server device 40, it is transmitted together.

  In addition, the application control unit 120, when the culture medium ID 66 and the work ID 71 that is the instruction ID 71a and the process ID 71b are stored in the IC tag, the operation unit 170, the display control unit 161, and the short-range wireless communication interface The medium ID 66 and the work ID 71 are acquired in conjunction with 140.

  When the server device 40 recognizes the medium ID 66 and the work ID 71 from the medium image data and the instruction sheet image data, the application control unit 120 transmits instruction sheet image data in addition to the medium image data as the medium information.

  Furthermore, the application control unit 120 may be realized as a function when the CPU (central processing unit) constituting the mobile terminal management control unit 190 executes an application in terms of implementation.

  The network communication unit 130 establishes a communication line with the server device 40 connected to the network 30 under the control of the application control unit 120 and the mobile terminal management control unit 190, and exchanges various data such as culture medium image data. Do.

  The short-range wireless communication interface 140 performs short-range wireless communication using an IC tag under the control of the application control unit 120 and the mobile terminal management control unit 190.

  The current position detection unit 150 recognizes the position of a GPS (Global Positioning System) satellite via the network 30 under the control of the application control unit 120 or the mobile terminal management control unit 190, and transmits a satellite transmitted from the GPS satellite. A signal (GPS signal) is detected.

  Then, the current position detection unit 150 calculates (that is, detects) coordinate values indicated by the latitude and longitude of the current position of the portable communication terminal device 10 based on the detected GPS signal. In addition, the current position detection unit 150 provides the calculated coordinate value to the application control unit 120 as position information.

  In addition, when the portable communication terminal device 10 has a telephone function or a short-range wireless function, the portable communication terminal device 10 is based on the direction and the radio field intensity received by the telephone base station or the like for the radio wave used for the telephone or the short-range radio. The current position of the portable communication terminal device 10 may be calculated (detected).

  The display unit 160 has an image display area of a predetermined size (for example, 5 inches, W480 × H960 pixels), is configured by a panel of liquid crystal elements or EL (Electro Luminescence) elements, and is generated by the display control unit 161. A predetermined image is displayed based on the display data. In particular, in the present embodiment, when the culture medium registration application is being executed, the display unit 160 is linked to the operation unit 170, and various displays, captured work instructions 70 and film-type culture media that have been imaged. 60 is displayed.

  The display control unit 161 generates drawing data necessary to cause the display unit 160 to draw a predetermined image under the control of the application control unit 120 or the mobile terminal management control unit 190, and the generated drawing data is displayed on the display unit. 160 is output.

  The operation unit 170 includes various confirmation buttons, operation buttons for inputting operation commands, a number of keys such as a numeric keypad, and a touch sensor provided on the display unit 160, and is used when performing each operation. It has become. Specifically, the operation unit 170 is used when performing operations for executing the various processes described above when the culture medium registration application is activated.

  In the present embodiment, the operation unit 170 can also be used when manually inputting the culture medium ID 66 directly.

  The timer 180 provides the application control unit 120 with the date and time when the image data generation unit 110 images the film-type medium.

  The portable terminal management control unit 190 is mainly configured by a central processing unit (CPU) and includes various input / output ports such as a key input port and a display control port, and the overall functions of the portable communication terminal device 10. In addition, overall functions for executing the information providing program are generally controlled.

[5] Server Device [5.1] Configuration of Server Device Next, the configuration of the server device 40 of this embodiment will be described with reference to FIG. FIG. 5 is a configuration diagram showing the configuration of the server device 40 of the present embodiment.

  As shown in FIG. 5, the server device 40 of the present embodiment communicates with a database 400 in which various information such as work information and culture medium information is stored, and the portable communication terminal device 10 and the administrator terminal device 20. A communication control unit 410, a data processing unit 420 that executes various processes such as a culture medium information registration process, a server management control unit 430 that controls each unit of the server device 40, a ROM / RAM 440 that is used to control each unit, and a time And a timer 450 used for management. The above-described units are connected to each other by a bus 41, and data transfer is performed between the components.

  The communication control unit 410 is a predetermined network interface, establishes a communication line with the portable communication terminal device 10 or the administrator terminal device 20, and exchanges various data with the portable communication terminal device 10 or the administrator terminal device 20. I do.

  The database 400 includes an HDD or an SSD, and includes a medium information database (hereinafter abbreviated as “medium information DB”) 401, a line information database (hereinafter abbreviated as “line information DB”) 402, and a lot information database (hereinafter referred to as “line information DB”). , “Lot information DB”) 403 and a work management database (hereinafter abbreviated as “work management DB”) 404. For example, each DB of the present embodiment constitutes a database according to the present invention.

The medium information DB 401 is a database in which medium information regarding the film-type medium 60 of the sample extracted for each process of each work line 80 is stored for each work ID 71 and medium ID 66. For example, in the culture medium information DB 401,
(1) Medium ID
(2) Imaged medium image data (3) Image capture time (4) Other information including flag information (5) Colony candidate specific information including feature amount relating to color of each colony candidate (6) Colony candidate imaged Information of each colony candidate including the feature amount of the detection pixel used for the image processing and the coordinate position of the film-type medium 60 (hereinafter referred to as “colony candidate information”).
(7) ID of each colony candidate to be a colony (ID when labeling) and the number of colonies (8) Based on the number of colonies, it is determined whether the film-type medium 60 (that is, the specimen) is normal or abnormal Results of medium determination (9) Work ID
Are recorded in association with each other.

  When the sample to be extracted is always extracted from the same process on the same work line 80 in the same lot, the work ID may be a lot ID, and the sample to be extracted is the same lot. However, when extracting from a different work line 80 or a different process, the line ID or the process ID 71b is obtained.

  The number of colonies and the result of medium determination in each medium information may be registered when the medium information is registered, or may be registered at a predetermined timing different from the registration timing of the medium information.

  Furthermore, when a plurality of imaging times of medium information of the same film type culture medium 60 are registered (that is, when there are a plurality of culture examination times described later), in the same culture medium ID 66, the culture medium at each culture examination time. Each medium information of an image, imaging time, the number of colonies, and medium determination is stored.

The line information DB 402 is a database in which line information regarding each work line 80 is stored for each line ID. For example, the line information DB 402 includes
(1) Line ID
(2) The process ID 71b of the process included in the work line 80 and the two types of data are recorded in association with each other. As the process type, a name for identifying each process such as material input, blending, filling, boiling, packaging, and packaging is used.

The lot information DB 403 is a database in which lot information regarding each lot is stored for each lot ID. For example, in the lot information DB 403,
(1) Lot ID (instruction ID 71a)
(2) Line ID of work line used for production or inspection of lots
(3) Lot work start time (4) Lot work end time (5) Five pieces of data of a work team of workers who perform lot work are recorded in association with each other.

The work management DB 404 is a database that stores various types of information related to work contents. For example, the work management DB 404 includes
(1) Work ID
(2) Line ID of the work line from which the sample is to be extracted (or line ID and process ID 71b)
(3) Type of sample (food) (product name, lot ID and / or lot name)
(4) Type of inspection (generic bacteria or bacteria to be detected such as E. coli)
(5) Culture conditions (dilution ratio, type of diluent, culture temperature, culture humidity and culture time)
(6) Culture start time (7) Time after a predetermined time has elapsed from the start of culture (hereinafter also referred to as “culture test time”)
(8) Culture location (for example, the position of the incubator number and the number of steps in the incubator)
(9) Work date and time (work start time and end time)
(10) Worker ID (and / or worker name)
Are recorded in association with each other.

  In addition, only a single time (that is, an end time for ending the culture of the specimen) may be set as the culture examination time, or after the statutory or predetermined time has elapsed from the culture start time, for example, the culture A plurality of times such as 24 hours from the start time, 48 hours, or 72 hours may be set.

The data processing unit 420 executes various data processing in accordance with the application recorded in the ROM / RAM 440. In particular, the data processing unit 420 executes a predetermined program,
(1) Operation management of the communication control unit 410;
(2) Medium information registration processing executed in conjunction with each portable communication terminal device 10;
(3) The colony and the non-colony are selected based on the feature amount of each pixel constituting the medium image, and the selection is corrected in conjunction with the administrator terminal device 20 to detect the number of colonies and the number of colonies. Colony detection determination processing for performing medium determination based on
(4) a process of registering the number of selected colonies including the corrected colony and the medium determination in the database 400;
(5) lot determination processing for determining a lot based on registered medium information;
(6) Report processing for outputting the determination result in a report format;
(7) Management and control of the database 400;
I do.

  Specifically, the data processing unit 420 includes a registration processing unit 421, a colony detection determination unit 422, an imaging data generation unit 423, a lot determination processing unit 424, and a report processing unit 425.

  For example, the registration processing unit 421 of the present embodiment constitutes an acquisition unit and a registration unit according to the present invention, and the colony detection determination unit 422 of the present embodiment includes the detection unit, the calculation unit, the selection unit, and the like of the present invention. A determination unit and a setting unit are configured. Further, for example, the imaging data generation unit 423 of the present embodiment constitutes a providing unit according to the present invention. Furthermore, details of the registration processing unit 421, colony detection determination unit 422, imaging data generation unit 423, lot determination processing unit 424, and report processing unit 425 of this embodiment will be described later.

  The server management control unit 430 is mainly configured by a central processing unit (CPU), and integrally controls each unit of the server device 40 by executing a program. Specifically, the server management control unit 430 performs login processing for each user and various other controls based on a login request from the portable communication terminal device 10 by a user operation.

  Various programs necessary for driving the server device 40 are recorded in the ROM / RAM 440. In particular, the ROM / RAM 440 includes information on determination criteria (hereinafter referred to as “medium determination criterion information”) used in performing each determination of medium determination (pass / fail determination), process determination, line determination, and lot determination of each film type medium 60. Etc.) is stored. The ROM / RAM 440 is used as a work area during execution of each program.

  The timer 450 is used for managing the time required when the medium information registration process and the lot determination process are executed.

[5.2] Registration Processing Unit Next, details of the registration processing unit 421 in the server device 40 of the present embodiment will be described.

  The registration processing unit 421 according to the present embodiment performs a medium information registration process for registering each medium information in the medium information DB 401 while being associated with the work ID 71 in conjunction with the portable communication terminal device 10. In particular, the registration processing unit 421 executes different medium information registration processing based on flag information indicating whether the registration is initial registration or post-culture registration included in the culture medium information.

  In addition, the registration processing unit 421 executes a correction process for correcting the medium information stored in the medium information DB 401 under the instruction of the administrator terminal device 20 in conjunction with the imaging data generation unit 423.

(Initial registration)
When the flag information indicating initial registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 performs a culture medium information registration process based on the initial registration.

  Specifically, when the registration processing unit 421 acquires the culture medium information via the communication control unit 410, the registration processing unit 421 specifies the culture medium ID 66 and the work ID 71 included in the culture medium information. In addition, the registration processing unit 421 searches the work management DB 404 based on the specified work ID 71, specifies the corresponding work information, and associates the acquired medium image data and imaging time with the specified work information. Is registered in the culture medium information DB 401.

  In particular, the registration processing unit 421 specifies the lot ID including the work line 80 from which the sample is extracted based on the acquired work ID 71, the line ID of the work line 80, the process ID 71b from which the sample is extracted, and the specification. The medium image data and the imaging time included in the medium information are registered in the medium information DB 401 in association with the lot ID, the line ID, and the process ID 71b.

  Then, the registration processing unit 421 registers the imaging time in the culture medium information DB 401 as the culture start time. As the culture start time, a preset time may be used instead of the imaging time on the assumption that the medium information registration process is executed at the time.

(Registration after culture)
When the flag information indicating post-cultivation registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 performs a registration process based on post-cultivation registration.

  Specifically, when acquiring the culture medium information via the communication control unit 410, the registration processing unit 421 identifies the culture medium ID 66 and the work ID 71 included in the culture medium information, as in the initial registration. Then, the registration processing unit 421 searches the work management DB 404 based on the identified work ID 71, identifies the corresponding work information, and registers the imaging time in the culture medium information DB 401 in association with the identified work information. To do. Note that the culture examination time may be a preset time instead of the imaging time on the assumption that the culture information registration process is executed at the same time as the culture start time.

  On the other hand, the registration processing unit 421 registers the acquired medium image data in the medium information DB 401 together with the analysis result or without analysis while causing the colony detection determination unit 422 to perform image analysis. The colony detection determination unit 422 performs colony detection, colony count, and medium determination by image analysis, and details of the image analysis will be described later.

[5.3] Colony Detection Determination Unit [5.3.1] Principle of Colony Detection Determination Unit Next, the principle of the colony detection determination unit 422 in the server device 40 of the present embodiment will be described.

  The colony detection determination unit 422 of the present embodiment acquires the medium image data transmitted from the portable communication terminal device 10 or the medium image data already registered in the medium information DB 401 under the control of the registration processing unit 421. Then, while detecting colonies imaged in the medium image of the acquired medium image data, the number of the colonies is detected, and the colony detection determination process for performing the medium determination is executed.

  Specifically, when the colony detection determination unit 422 according to the present embodiment acquires a medium image, the colony detection determination unit 422 extracts a feature amount of each pixel constituting the acquired medium image such as RGB gradation values, luminance values, or saturation. At the same time, a colony and a non-colony are selected based on the extracted feature amount of each pixel, and a colony and a non-colony with a high possibility of erroneous detection are discriminated. In addition, the colony detection determination unit 422 corrects the determined colony or non-colony in conjunction with the administrator terminal device 20, executes colony and its number detection, and medium determination based on the number of colonies, and registers This is provided to the processing unit 421.

In particular, when the colony detection determination unit 422 extracts the feature amount of each pixel constituting the medium image,
(1) Colony candidate detection processing for detecting a pixel having a predetermined condition from each pixel based on the extracted feature amount of each pixel and detecting a colony candidate based on each detected pixel;
(2) For each detected colony candidate, likelihood calculation for calculating the likelihood of each colony candidate based on the characteristics of the colony candidate determined by each pixel constituting the image when each colony candidate is imaged processing,
(3) A colony selection process for comparing the calculated likelihood of each colony candidate with a colony selection threshold and selecting each colony candidate into a colony and a non-colony.
(4) Processing for discriminating colony and non-colony types based on likelihood in order to present a colony or non-colony with a high possibility of erroneous detection while allowing the administrator to check the selected colonies and non-colony ( Hereinafter, it is referred to as “false positive colony discrimination process”.)
(5) Based on the administrator's instruction transmitted from the administrator terminal device 20, the colony correction that corrects the colony to a non-colony or the non-colony colony while interlocking with the imaging data generation unit 423 is performed. processing,
(6) Counting process for counting the number of colonies as the number of colonies based on the administrator's instruction (determination instruction described later),
(7) Based on the counted number of colonies, the pass / fail determination of the specimen for each film-type medium 60 (ie, medium determination),
Execute.

  Usually, in a colony candidate, when likelihood is high, possibility as a colony is high, and when likelihood is low, possibility as a colony is low. On the other hand, when selecting colonies and non-colonies such as garbage, a standard is set for the likelihood according to a predetermined threshold, and the colony or non-colony is selected before and after that, but the likelihood with a value close to the threshold is selected. In the colony candidate to have, it is assumed that overdetection as a colony or non-detection due to non-colony occurs.

  Therefore, in the present embodiment, the colony or non-colony is classified into two or more types based on the likelihood indicating the degree of likelihood of colony, and the characteristics of the colony and the colony that are close to the characteristics of the non-colony are determined according to a uniform standard. It is possible to discriminate between colonies that are likely to be erroneously detected and non-colony types such as non-colonies that are close to each other. Details of each process of the colony detection determination unit 422 will be described below.

[5.3.2] Details of Each Process of Colony Detection Determination Unit Next, details of each process of the colony detection determination unit 422 in the server device 40 of the present embodiment will be described with reference to FIGS. To do.

  6 is a diagram for explaining likelihood calculation processing executed in the server device 40 of the present embodiment, and FIG. 7 explains colony selection processing executed in the server device 40 of the present embodiment. It is a figure for doing. Moreover, FIG. 8 is a figure for demonstrating the false detection colony discrimination | determination process performed in the server apparatus 40 of this embodiment, and FIG. 9 shows the colony correction process performed in the server apparatus 40 of this embodiment. It is a figure for demonstrating.

(Colony candidate detection process)
The colony detection determination unit 422 binarizes the feature amount of each pixel of the culture medium image using a predetermined threshold as a predetermined condition, and images a pixel having one value, that is, a colony. Pixels that are assumed to be used for this purpose (hereinafter referred to as “detection pixels”) are extracted.

  Then, the colony detection determination unit 422 performs image filtering processing for reducing noise, connects adjacent detection pixels, performs labeling, assigns an ID, and sets the connected detection pixel group as one object. That is, it detects as a colony candidate.

  In particular, the colony detection determination unit 422 includes, as image filter processing, an expansion process that replaces all pixels adjacent to a detection pixel with detection pixels when other detection pixels exist adjacent to each detection pixel, and When there is a non-detected pixel adjacent to the detection pixel (hereinafter referred to as “non-detection pixel”), a contraction process is performed to replace all the pixels adjacent to the detection pixel with non-detection pixels.

  The colony detection determination unit 422 temporarily stores the extracted detection pixels in the ROM / RAM 440.

(Likelihood calculation process)
The colony detection determination unit 422, for each detected colony candidate, as a characteristic of the detected colony candidate, when imaging each detected colony candidate, a feature amount relating to the color of each pixel constituting the image, the medium of each pixel The likelihood of each colony candidate is calculated based on the arrangement position on the image or both. Then, the colony detection determination unit 422 temporarily stores the calculated likelihood of each colony candidate in the ROM / RAM 440.

Specifically, the colony detection determination unit 422 determines whether each colony candidate is
(1) An average value of feature amounts relating to each detected pixel color belonging to a colony candidate, such as RGB gradation value, luminance value, saturation, or a combination of the two,
(2) The roundness of the colony based on the coordinates of the center of gravity of the colony candidate, and
(3) Edge strength indicating the strength of the edge at the boundary of colony candidates,
Are respectively added using a weighting coefficient, and a value obtained by normalizing a value indicating the addition result is calculated as a likelihood.

  For example, the colony detection determination unit 422 calculates the addition result “f (Ai)” based on (Expression 1), normalizes the calculated value based on (Expression 2), and calculates the likelihood “P (Ai)”. Is calculated.

  The coefficients “α”, “β”, and “γ” are weighting coefficients, and “σ” is a predetermined coefficient. “Fc (Ai)”, “fs (Ai)”, and “fe (Ai)” are the average values of the respective feature amounts of the colony candidates in the “i” -th (colony candidate index number (ID)), colonies The value when adding the roundness and the edge strength is shown. In particular, in (Expression 2), P (Ai) approaches “1” when the likelihood increases, and approaches “0” when the likelihood decreases.

  On the other hand, the colony detection determination unit 422 determines, as the roundness, the distance Ra from the coordinates of the center of gravity of the colony candidate to the outermost detection pixel at the shortest distance on the film type medium 60 and the coordinates of the center of gravity of the colony candidate. A value calculated according to (Equation 3) based on the ratio to the distance Rb to the outermost detection pixel at the longest distance on the mold medium 60 or the area “S” of the colony and the length “L” of the outer periphery Use “M”.

  On the other hand, the colony detection determination unit 422 calculates the average of the difference in luminance, saturation, or RGB gradation value between the outermost detection pixels and the non-detection pixels adjacent to the outermost detection pixels as the edge strength. Use.

  For example, when colony candidates A1 to A7 are detected, the colony detection determination unit 422, as shown in FIG. 6, the average value of the feature values related to the detected pixel colors belonging to the colony candidate, the colony candidate Based on the roundness of the colony based on the coordinates of the center of gravity and the edge strength indicating the strength of the edge at the boundary of the colony candidate, the likelihood is calculated, and the ID of the colony candidate, its center of gravity coordinates, and the likelihood are calculated. Correspondingly, it is temporarily stored in the ROM / RAM 440.

(Colony selection process)
The colony detection determination unit 422 compares the calculated likelihood of each colony candidate with the colony selection threshold, and selects a colony candidate having a likelihood equal to or higher than the colony selection threshold as a colony, and the colony selection threshold A colony selection process for selecting a colony candidate having a lower likelihood as a non-colony is executed.

  For example, as shown in FIG. 6 described above, when the likelihood of a colony candidate is calculated and the colony selection threshold is “0.5”, the colony detection determination unit 422 displays the colony detection determination unit 422 in FIG. As shown, the colony candidates A1 to A4 and A6 are selected as colonies, and the colony candidates A5 and A7 are selected as non-colonies.

  In addition, the colony detection determination part 422 is linked with the administrator terminal device 20, and executes a colony selection process again when a correction instruction for the colony selection threshold is received based on an instruction from the administrator at the time of correction registration.

(Error detection colony discrimination process)
The colony detection determination unit 422 allows the administrator to easily confirm a colony or non-colony with a high possibility of erroneous detection based on a likelihood (i.e., a non-colony actually non-colony). A colony candidate that is a colony that is over-detected) or a non-colony (that is, a colony candidate that is actually a colony but has not been detected) is determined.

  Specifically, for each colony candidate selected as a colony by the colony selection process, the colony detection determination unit 422 compares the likelihood of the colony candidate with a colony false detection confirmation threshold, and is equal to or greater than the colony selection threshold. First, a colony candidate having a likelihood is discriminated as a first colony, and a first discrimination process for discriminating a colony candidate having a likelihood lower than the colony selection threshold as a second colony is executed.

  For example, as shown in FIG. 6 and FIG. 7 described above, when the colony candidates A1 to A4 and A6 are selected as colonies and the threshold for erroneous colony detection confirmation is “0.6”, As shown in FIG. 8, colony candidates A1 to A3 and A8 selected as colonies are determined as first colonies, and similarly, a colony candidate A4 selected as colonies is determined as a second colony.

  On the other hand, for each colony candidate selected as a non-colony by the colony selection process, the colony detection determination unit 422 compares the likelihood of the colony candidate with a non-colony false detection confirmation threshold, and is equal to or less than the non-colony selection threshold. A colony candidate having a likelihood is discriminated as a first non-colony, and a second discrimination process for discriminating a colony candidate having a likelihood higher than the non-colony selection threshold as a second non-colony is executed.

  For example, as shown in FIG. 6 and FIG. 7 described above, when the colony candidates A5 and A7 are selected as non-colonies and the threshold for erroneous colony detection confirmation is “0.4”, As shown in FIG. 8, the colony candidate A7 selected as the non-colony is determined as the first non-colony, and the colony candidate A5 similarly selected as the non-colony is determined as the second non-colony.

  Note that the colony detection determination unit 422 is linked to the administrator terminal device 20, and when the colony selection process is re-executed or based on an instruction from the administrator at the time of correction registration, a colony detection error detection threshold or non-colony detection When the confirmation threshold value correction instruction is received, the first determination process or the second determination process is executed again.

(Colony correction process)
The colony detection determination unit 422 includes the image data generated by the imaging data generation unit 423, performs data transfer in conjunction with the administrator terminal device 20, and if there is an overdetected colony, the corresponding colony ( Specifically, the second colony is corrected to a non-colony, and when there is an undetected colony, a colony correction process is executed to correct the corresponding non-colony (specifically, the second non-colony) to a colony. .

  Specifically, when the colony detection determination unit 422 receives an administrator correction instruction from the administrator terminal device 20, the colony detection determination unit 422 acquires the colony candidate ID transmitted together with the correction instruction, and has the colony candidate ID. When the colony candidate is selected as the second colony, the colony candidate attribute is corrected from the colony to the non-colony, and the colony candidate having the colony candidate ID is selected as the second non-colony. Corrects the attribute of the colony candidate from non-colony to colony.

  If the colony candidate specified by the colony candidate ID transmitted with the correction instruction is the first colony or the first non-colony, the colony detection determination unit 422 corrects the attribute of the colony candidate without correcting the attribute of the colony candidate. The image data generation unit 423 is caused to generate image data for presenting that correction is impossible.

  For example, as shown in FIG. 8 above, the colony candidates A1 to A3 and A8 are the first colony, the colony candidate A4 is the second colony, the colony candidate A7 is the first non-colony, and the colony candidate A5 is the second non-colony. And if the correction instruction | indication of colony candidate A4 is received, the colony detection determination part 422 will correct the colony candidate A4 to a non-colony (namely, 1st non-colony), as shown in FIG.

  Note that the colony detection determination unit 422 may execute correction to a non-colony or correction to a colony based on an instruction from the administrator even for the first colony or the first non-colony.

(Counting the number of colonies)
The colony detection determination unit 422, based on the administrator's instruction (that is, the confirmation instruction) or after executing the colony correction process by the imaging data generation unit 423, the colony candidate selected as the colony, that is, the first The number of colony candidates selected as one colony and the second colony is counted as the number of colonies.

(Sample pass / fail judgment)
The colony detection determination unit 422 performs medium determination (that is, determination of pass / fail of the specimen) as to whether the specimen of each film type culture medium 60 is abnormal or normal. For example, the registration processing unit 421 determines pass / fail of each sample based on a threshold value of the number of bacteria set in advance according to the bacterial species to be detected from the sample (that is, a determination criterion), and the result is stored in the registration processing unit 421. provide.

  In particular, in the present embodiment, since the determination criteria are different based on the bacterial species and the dilution rate, the registration processing unit 421 obtains the medium determination criterion information from the ROM / RAM 440 based on the criteria used when cultivating the specimen and the bacterial species. Read out and perform medium determination.

  Each criterion information is specified in association with the culture medium ID 66. That is, since the type of the film-type medium 60 is different for each bacterial species detected from the specimen, the bacterial species to be detected can be specified by specifying the medium ID 66. Therefore, in this embodiment, the determination reference information specified based on the culture medium ID 66 can be read out by setting the dilution rate in advance.

  Moreover, in this embodiment, since the pass / fail determination of each film type culture medium 60 is used in the process determination, the line determination, and the lot determination, the medium determination is executed in association with these determinations. In addition, the method of pass / fail determination of each film type culture medium 60 in process determination, line determination, and lot determination will be described later.

[5.4] Imaging Data Generation Unit The imaging data generation unit 423 is configured to display the second colony, the second non-colony, or both together with the first colony in a predetermined display mode under the control of the colony detection determination unit 422. Image data is generated for imaging on the image, and image data including the medium image generated via the communication control unit 410 is provided to the administrator terminal device 20.

  Further, when the colony correction process is executed, the imaging data generation unit 423 generates an image that superimposes a predetermined image on the medium image based on the correction instruction from the administrator, and includes the generated medium image The image data is provided to the administrator terminal device 20.

  For example, as shown in FIG. 8, the imaging data generation unit 423 generates image data that can display each colony candidate as a first colony, a second colony, and a second non-colony in different display modes, and corrects the colony After the processing, as shown in FIG. 9, the colony candidate A4 is corrected to a non-colony, and image data that can be displayed in different display modes as the first colony, the second colony, and the second non-colony are generated. The administrator terminal device 20 is provided with image data including the generated culture medium image.

[5.5] Lot Determination Processing Unit Next, details of the lot determination processing unit 424 in the server device 40 of the present embodiment will be described.

  The lot determination processing unit 424 of the present embodiment analyzes data on hygiene management in the designated lot, work line 80 or process based on the instructions of the administrator input to the administrator terminal device 20 (ie, pass / fail determination). Execute.

  Specifically, the lot determination processing unit 424, for each lot, line information of the work line 80 used for the corresponding lot, and medium information registered in association with the corresponding work line 80 (at least the number of colonies). Based on the above, it is determined whether the food group manufactured or inspected under the same condition has a predetermined condition.

  In addition, when the lot determination is instructed, the lot determination processing unit 424 determines whether the work line 80 included in the corresponding lot and the processes belonging to the work line 80 are abnormal or normal in terms of hygiene management. 80 line determinations (that is, pass / fail determination for hygiene management) and process determinations for each process (that is, determination of pass / fail for hygiene management) are performed, and lot determination is performed using the process determination and line determination.

  In particular, when the lot determination is instructed, the lot determination processing unit 424, for example, the number of colonies of the film-type medium 60 in the medium information that has passed a predetermined culture inspection time such as 24 hours or 48 hours from the start of culture, Based on the medium information of the pass / fail determination of the specimen, the pass / fail of hygienic management of the process is determined, and the pass / fail of hygiene management of the work line 80 is determined based on the pass / fail determination of the process included in the corresponding work line 80. Based on the pass / fail determination of the work line 80 included in the corresponding lot, the pass / fail in sanitary management of the lot is determined.

  Note that the lot determination processing unit 424 of the present embodiment may execute not only lot determination but also process determination or line determination as data analysis.

(Process judgment)
The lot determination processing unit 424 has a culture start time at the same timing, a culture examination time at the same timing, a culture start time having a time within a preset time from the first time to the second time, or a preset first time. The culture start time or the culture test time has a predetermined condition, such as having a culture test time having a time within the time from the first time to the second time, and is registered in association with the same process in the same lot. Each process determination is performed based on the culture medium information of the plurality of film-type culture media 60 that has been performed. That is, the lot determination processing unit 424 executes each process determination by comprehensive determination based on each medium determination result of the plurality of film-type mediums 60.

  Specifically, the lot determination processing unit 424 displays the medium determination results of the corresponding film-type medium 60 (that is, all the medium determination results registered in the medium information having the process ID 71b of the process to be subjected to the process determination). In addition to obtaining, if the number of pass / fail of the judgment result satisfies a predetermined condition, the process judgment of the process to be judged is judged to be acceptable, and if not, the judgment should be made The process judgment of the process is judged as rejected.

  For example, the lot determination processing unit 424 determines that the conditions for all the film-type culture media 60 when the acceptable film-type culture media 60 are a predetermined ratio (60%) or more in the individual film-type culture media 60 in the corresponding process ( The number of colonies is equal to or less than a certain number), or the condition that the number of colonies of each film-type medium 60 corresponds (the average number of colonies of a single film-type medium 60 is equal to or less than a certain number). In this case, the corresponding process is determined to be acceptable.

  Note that the lot determination processing unit 424 of the present embodiment can change conditions depending on the type of specimen, the type of bacteria to be detected, etc., and can be set by an administrator or provided as part of a program. The

(Line judgment)
The lot determination processing unit 424 performs line determination for each work line 80 based on the pass / fail determination for hygiene management of a plurality of processes that are determined at a predetermined timing and registered in association with the corresponding line information. To do. That is, the lot determination processing unit 424 performs line determination by comprehensive determination based on the determination results of a plurality of processes of the same lot determined at a predetermined timing.

  Specifically, the lot determination processing unit 424 is determined at the same timing or a timing satisfying a predetermined condition, and has the line ID of the work line 80 to be subjected to line determination as described above. When the determination result of the determination is acquired and the number of pass / fail of the determination result satisfies a predetermined condition, the line determination of the work line 80 to be determined is determined to be acceptable, and the condition is not satisfied The line determination of the work line 80 to be determined is determined to be unacceptable.

  For example, when the three processes have the line ID of the work line 80 to be determined, the first process is “pass”, the second process is “pass”, and the third process is “fail”. In the case where the work line 80 is configured from a process in which the predetermined condition is “60%”, the lot determination processing unit 424 passes the line determination of the work line 80 to be determined.

(Lot judgment)
The lot determination processing unit 424 executes the lot determination for each lot based on the hygiene management pass / fail determination of the plurality of work lines 80 that are determined at a predetermined timing and registered in association with the corresponding lot information. To do. That is, the lot determination processing unit 424 executes lot determination by comprehensive determination based on the determination results of the plurality of work lines 80.

  Specifically, the lot determination processing unit 424 executes the lot determination based on the pass / fail determination in the work line 80 having the lot ID of the lot for which the lot determination is to be performed.

  For example, the lot determination processing unit 424 determines that the lot determination of the lot to be determined is acceptable when the line determination is successful in 60% of the work lines 80 having the lot ID, and the three work lines 80 determine the lot to be determined. Assume that a lot ID is included. In this case, when the first work line 80 is “pass”, the second work line 80 is “pass”, and the third work line 80 is “fail”, the lot determination processing unit 424 determines the lot to be determined. It is determined that the lot determination is acceptable.

  When the lot work time is long, the lot determination may be executed by dividing the time every predetermined time (for example, 8 hours or 12 hours). In this case, when the lot determination is performed while the work is suspended, a dummy result may be used, or it may be determined that the work is being suspended.

(Other)
The lot determination processing unit 424 can perform determinations and data analysis other than those described above using the culture medium information, line information, lot information, and work management DB 404, and the determination result or data analysis via the report processing unit 425. The result can be provided to the administrator terminal device 20 so that the administrator can view the result.

  Specifically, the lot determination processing unit 424 checks the reliability of the specimen cultured in each film-type medium 60, that is, checks whether or not an inspection error has occurred. The medium information in the medium ID is subjected to a predetermined analysis of the medium information or the medium image data in time series from the culture start time to the end of the culture.

  For example, the lot determination processing unit 424 has a specific culture medium ID (same culture medium ID) 66 based on an instruction from the administrator received via the administrator terminal device 20, and starts from the culture start time to a predetermined time. Are extracted together with the medium image data. Then, the lot determination processing unit 424 performs a predetermined analysis on each medium information and each image data along a predetermined time series, or aggregates each medium information and each image data along a time series. In addition, the lot determination processing unit 424 causes the report processing unit 425 to generate the analysis result or the aggregation result as browsing data having a predetermined data format, and allows the administrator to browse the generated browsing data. 20 to provide.

  Note that the lot determination processing unit 424 is linked to the report processing unit 425, and at this time, based on an instruction from the manager terminal device 20, each medium information in one medium ID 66 is used for legal hygiene management reporting. May be made individually viewable.

  The lot determination processing unit 424 may have a search function for medium information, line information, lot information, or work management information. Specifically, the lot determination processing unit 424 uses the lot ID (instruction ID 71a), the work line ID, the process ID 71b, the work ID 71, the culture start time, the lot work date and time, the culture medium ID 66, the specimen type, and the like as search keys. The database 400 may be searched, and the report processing unit 425 may be linked to generate data that allows the administrator to view the corresponding medium information and various types of information.

  Note that the report processing unit 425 is configured to search for medium information (medium image data, number of colonies and medium determination result), process information, and line specified by a search based on an instruction from the administrator received via the administrator terminal device 20. It is also possible to provide information or information on lots in a predetermined report format. That is, the report processing unit 425 is configured to be able to provide various information as evidence or reports in legal inspection reports and other cases.

[5.6] Report Processing Unit Next, details of the report processing unit 425 in the server device 40 of the present embodiment will be described.

  The report processing unit 425 performs data analysis related to hygiene management in the designated lot, work line 80 or process based on the administrator's instruction input to the administrator terminal device 20 and template data having a predetermined report format. An analysis result (that is, a determination result of pass / fail determination) is generated as report data, and the generated report data is provided to the administrator terminal device 20 so as to be viewable by the administrator via the communication control unit 410.

  Specifically, the report processing unit 425 extracts necessary information from the medium information, the line information, and the lot information such as the medium image data, the number of colonies, or the medium determination result, and the extracted information and the lot determination processing unit 424. Based on the analysis result obtained by the above, report data is generated while assigning each information to the template data.

  Note that, as described above, the report processing unit 425 is configured to generate predetermined report data or browsing data in accordance with various analyzes or searches and provide the data to the administrator terminal device 20. . In other words, the report processing unit 425 can also provide medium information, process information, line information, or lot-related information specified by the search in a predetermined report format based on the received administrator's instruction. In addition, it is configured so that various information can be provided as evidence or reports in statutory inspection reports and other cases.

[6] Hygiene management system operation process [6.1] Medium information registration process (portable communication terminal device)
Next, operation | movement of the culture medium information registration process in the portable communication terminal device 10 of this embodiment is demonstrated using FIG. FIG. 10 is a flowchart showing the culture information registration processing operation in the portable communication terminal device 10 of the present embodiment.

  In this operation, it is assumed that corresponding lot information and line information associated with the work instruction sheet 70 are already stored in the line information DB 402 and the lot information DB 403.

  Further, it is assumed that the medium ID or instruction ID is attached to the film-type medium 60 and the work instruction sheet 70 by a two-dimensional barcode.

  Further, in this operation, the number of colonies in each film-type medium 60 is detected at the time of registration in the medium information database 400, and the portable communication terminal device 10 uses RGB for capturing a medium image. It is assumed that calibration for the gradation value or the luminance value is performed.

  First, in the portable communication terminal device 10, when the application control unit 120 detects an activation instruction for the culture medium registration application via the operation unit 170 (step S101), the application control unit 120 reads the culture medium registration application from the application storage unit 101 and activates it ( Step S102). At this time, the application control unit 120 performs initialization of the work memory and other necessary processes under the control of the mobile terminal management control unit 190.

  Next, the application control unit 120 displays an image for interlocking with the display control unit 161 to select whether the initial registration of the film-type medium 60 or the post-culture registration after a predetermined time has elapsed from the start of the culture. It is displayed on the unit 160 and waits for its input (step S103).

  Next, when detecting an input of a selection instruction from the operation unit 170 (step S104), the application control unit 120 determines whether initial registration or post-cultivation registration and sets the result in flag information (step S105).

  Next, the application control unit 120 causes the display unit 160 to display a screen that prompts the imaging of the film-type medium 60, and waits for imaging by the image data generation unit 110 in conjunction with the operation unit 170 (step S106). At this time, the application control unit 120 causes a predetermined image to be superimposed on the display unit 160 in order to align the medium image to be acquired.

  Next, when the application control unit 120 detects imaging by the image data generation unit 110 in conjunction with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S107), the image data generation unit 110 causes the medium ID 66 to be detected. At the same time, the imaged medium image data of the film type medium 60 is acquired and stored in the image data storage unit 102 while giving a predetermined image ID (step S108).

  Next, the application control unit 120 acquires the current time from the timer 180 as the imaging time of the medium image data, and stores it in the image data storage unit 102 as metadata in association with the medium image data (step S109).

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in a predetermined area of the acquired imaged culture medium image data, acquires the culture medium ID 66, and stores the image data in association with the culture medium image data. The information is stored in the unit 102 (step S110).

  Next, the application control unit 120 causes the display unit 160 to display a screen for acquiring the instruction sheet image data together with the instruction sheet ID 71a and the process ID 71b in conjunction with the display control unit 161, and waits for acquisition of the instruction sheet image data. (Step S111).

  Next, when the application control unit 120 detects imaging by the image data generation unit 110 in cooperation with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S112), an instruction in which the work ID 71 is imaged. The book image data is acquired and stored in the image data storage unit 102 in association with the culture medium image data acquired in step S108 (step S113).

  In addition, when acquiring the process ID 71b as the work ID 71, or when acquiring the one process ID 71b from the plurality of process IDs 71b, the application control unit 120 operates in conjunction with the display control unit 161 and the operation unit 170, and the user The process ID 71b to be acquired is displayed, and the corresponding process ID 71b is acquired.

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in the predetermined region of the captured instruction image data and the medium image data, acquires the work ID 71, and corresponds to the instruction image data In addition, the image data is stored in the image data storage unit 102 (step S114).

  Next, the application control unit 120 acquires the current time as the imaging time of the medium image data from the timer 180, and in conjunction with the display control unit 161 and the operation unit 170, the application control unit 120 acquires the acquired instruction image data, work ID 71, The medium image data, medium ID 66, and imaging time are confirmed (step S115).

  Note that the application control unit 120 may re-acquire the culture image data based on an instruction from the operator when the acquired medium information is confirmed by the operator. At this time, the application control unit 120 may be able to correct the medium image data by the operator in conjunction with the display control unit 161 and the operation unit 170.

  Next, the application control unit 120, based on the operator's instruction, together with flag information indicating whether it is initial registration or post-culture registration, terminal ID, and work ID 71, the acquired medium image data, its metadata, and whether it is initial registration. Flag information indicating whether it is registered after culture is transmitted as medium information to the server device 40 via the network communication unit 130 (step S116), and the registration process of the portable communication terminal device 10 is terminated.

  At this time, when transmitting the culture medium information, the network communication unit 130 executes login for accessing the server device 40 based on the terminal ID or the input ID and password, and the server device 40 is completed after the login is completed. Communication line is established and medium information is transmitted.

[6.2] Medium information registration processing (server device)
Next, the culture information registration processing operation in the server device 40 of the present embodiment will be described with reference to FIG. In addition, FIG. 11 is a flowchart which shows the registration processing operation | movement of the culture medium information in the server apparatus 40 of this embodiment.

  In addition, this operation | movement is a process performed in conjunction with the culture medium information registration process performed by the portable communication terminal device 10, and the server apparatus 40 uses the culture medium information transmitted from the portable communication terminal apparatus 10 as a culture medium. This is a process for registering in the information DB 401.

  First, in the server device 40, when the communication control unit 410 receives the culture medium information transmitted from the portable communication terminal device 10 (step S300), the registration processing unit 421 is based on the flag information included in the received culture medium information. Whether the registration is initial registration or post-culture registration is determined (step S301).

  At this time, if the registration processing unit 421 determines initial registration, the process proceeds to step S302. If the registration processing unit 421 determines that it is not initial registration, that is, if it is determined to be post-culture registration, step S304 is performed. Move on to processing.

  Next, when it is determined as initial registration, the registration processing unit 421 extracts and specifies the work ID 71 included in the culture medium information (step S302).

  Next, the registration processing unit 421 registers the culture medium information in the culture medium information DB 401 based on the identified work ID 71 (step S303), and ends this operation. Although the culture start time is stored in advance in the work management DB 404, the registration processing unit 421 registers the imaging time included in the received medium information as the culture start time in the work management DB 404 in the process of step S303. Also good.

  On the other hand, if the registration processing unit 421 determines that the registration is after culture, it extracts and specifies the work ID 71 included in the culture medium information (step S304), and registers the culture medium information based on the imaging time included in the culture medium information. The culture examination time to be specified is specified (step S305).

  At this time, the registration processing unit 421 may be registered by designating a culture examination time for registering the culture medium information in conjunction with the portable communication terminal device 10, or the imaging time included in the received culture medium information. The culture examination time may be registered as it is. In addition, the registration processing unit 421 manages the culture medium registration at the culture test time that has already been performed, and specifies the culture test time based on the timing at which the culture medium information is received and the culture test time that has already been used to register the culture medium information. May be. That is, the registration processing unit 421 may specify the culture examination time by rewriting the execution flag each time the medium information is registered and comparing the received imaging time with reference to the information of the flag.

  Next, the registration processing unit 421 registers the culture image data, the recognized culture medium ID 66, and the culture start time information with the imaging time included in the culture medium information as the culture start time in association with the specified work ID 71 in the culture medium information DB 401. (Step S309), and this operation is terminated.

  The registration processing unit 421 displays line information and information to be registered on the display unit 160 of the portable communication terminal device 10, and based on a user instruction input via the portable communication terminal device 10, the culture medium Information or the like may be registered.

  Further, when the culture medium image data is transmitted from the portable communication terminal apparatus 10 and the medium ID 66 is recognized by analyzing each image data by the server apparatus 40, the registration processing unit 421 receives the reception in the process of step S301. The medium ID 66 imaged on each predetermined area of the medium image data thus obtained is analyzed, and the medium ID 66 is recognized and acquired. Similarly, the server device 40 also recognizes the work ID 71 in the instruction image data.

[6.3] Colony Detection Determination Processing Including Analysis Result Acquisition Display Processing Next, analysis results executed by the administrator terminal device 20 in the server device 40 of the present embodiment with reference to FIGS. 12 and 13. The operation of the colony detection determination process including the acquisition display process will be described. 12 and 13 are flowcharts illustrating the operation of the colony detection determination process including the analysis result acquisition display process executed by the administrator terminal device 20 in the server device 40 of the present embodiment.

  This operation is executed under the instruction of the administrator terminal device 20, and a luminance value is used as a feature value of each pixel in order to select colonies and non-colonys. This operation may be started by an instruction from the administrator, or after the above-described medium information registration process, an inquiry from the server device 40 (that is, execution of the colony detection determination process in the registered medium information) Execution may be started by responding to the presence / absence inquiry.

  First, the administrator terminal device 20 activates a predetermined application and receives an instruction to execute colony detection while specifying medium information (hereinafter referred to as “colony detection instruction”), or a server device. When an execution instruction by the administrator is received in response to an inquiry as to whether or not to execute the colony detection determination process based on the culture medium information registered from 40 (step S401), the server apparatus 40 includes the culture medium ID 66 of the specified culture medium information. A colony detection instruction is transmitted (step S402).

  Note that the administrator terminal device 20 may be specified by the administrator's selection in conjunction with the server device 40 in specifying the culture medium ID 66, or the execution of the colony detection determination process transmitted from the server device 40 may be performed. It may be specified by responding to the presence / absence inquiry. In addition, when the administrator terminal device 20 transmits the colony detection instruction, the administrator terminal device 20 waits for an inquiry regarding the execution of the colony correction process.

  Next, in the server device 40, when the communication control unit 410 receives an instruction to execute colony detection transmitted from the administrator terminal device 20 (step S501), the registration processing unit 421 includes the culture medium information included in the colony detection instruction. And instructing the colony detection determination unit 422 to start executing the colony detection determination process based on the read medium information (step S502). Specifically, the registration processing unit 421 reads the medium information of the medium ID 66 and the medium image data included in the colony detection instruction, and executes the colony detection determination process to the colony detection determination unit 422 while providing the read medium information. Let it begin.

  Next, the colony detection determination unit 422 executes a luminance value extraction process for extracting the luminance value of each pixel constituting the medium image acquired from the medium image data (step S503).

  Next, the colony detection determination unit 422 detects, as a detection pixel, a pixel having a luminance value equal to or higher than the threshold based on a predetermined threshold, and binarizes the detection pixel and a pixel constituting the culture medium. The process is executed (step S504).

  Next, the colony detection determination unit 422 detects colony candidates based on the detected pixels detected (step S505). Specifically, the colony detection determination unit 422 detects colony candidates by connecting adjacent detection pixels while performing image filter processing and labeling processing on adjacent detection pixels among the detected pixels.

  At this time, the colony detection determination unit 422 assigns an ID to each colony candidate through a labeling process. In addition, the colony detection determination unit 422 determines the number of detection pixels, the luminance value of each detection pixel, the coordinate position of each detection pixel on the film-type medium 60, the position of the center of gravity of each pixel, and the like for each colony candidate. Colony candidate information indicating candidate characteristics is acquired.

  Next, when the colony detection determination unit 422 images each detected colony candidate, the brightness value of each pixel constituting the image, the arrangement position of each pixel on the medium image, or both detected colony candidates Based on the characteristics, likelihood calculation processing for calculating the likelihood of each colony candidate is executed (step S506).

Specifically, the colony detection determination unit 422
(1) An average value of feature amounts related to detected pixel colors belonging to colony candidates,
(2) The roundness of the colony based on the coordinates of the center of gravity of the colony candidate, and
(3) Edge strength indicating the strength of the edge at the boundary of colony candidates,
And the likelihood of each colony candidate is calculated based on these.

  Next, the colony detection determination unit 422 selects colony and non-colony detected colonies based on the colony selection threshold set in advance or set by the administrator's instruction and the calculated likelihood of each colony candidate. A colony selection process is performed for selection (step S507).

  Next, the colony detection determination unit 422 is based on the likelihood of each colony candidate calculated in advance and the threshold for erroneous colony detection confirmation and the threshold for non-colony erroneous detection confirmation set according to the administrator's instruction. The first discrimination process for discriminating the colony into the first colony and the second colony is executed, and the second discrimination process for discriminating the non-colony into the first non-colony and the second non-colony is executed (step S508).

  Next, the imaging data generation unit 423 generates the first colony together with the first colony based on the medium information regarding the colony candidate including the likelihood and the types of the first colony, the second colony, the first non-colony, and the second non-colony. Image data for allowing the administrator with different display modes to view the two colonies and the second non-colony is generated, and the generated image data is transmitted to the administrator terminal device 20 (step S509).

  Next, when the administrator terminal device 20 receives the image data transmitted from the server device 30 (step S411), colonies and non-colonies are marked while colony candidates are marked on the medium image by the image data according to a predetermined display mode. The images sorted (hereinafter also referred to as “screened images”) are displayed so as to be viewable to the administrator, and the administrator's instruction is waited (step S412).

  Next, when the administrator terminal device 20 receives the administrator's instruction (step S413), the administrator's instruction is a colony or non-colony correction instruction (hereinafter referred to as “colony correction instruction”) or a change in each threshold value. It is determined whether it is an instruction (hereinafter referred to as “threshold change instruction”) or a confirmation instruction for confirming the first colony and the second colony as colonies (hereinafter referred to as “colony confirmation instruction”) (step S414).

  At this time, if the administrator terminal device 20 determines that the administrator instruction is a colony correction instruction or a threshold value change instruction (hereinafter also simply referred to as “correction instruction”), the administrator terminal apparatus 20 transmits the instruction to the server device 40. (Step S415) The process proceeds to step S411, and if the administrator's instruction is determined to be a colony confirmation instruction, the instruction is transmitted to the server device 40 (step S416), and the process proceeds to step S421. .

  The administrator terminal device 20 transmits a colony correction instruction including the ID of a colony candidate to be corrected to the server device 40, and transmits a threshold type to be changed and a threshold value change instruction including the value to the server device 40.

  Next, in the server device 40, when the communication control unit 410 receives an administrator instruction transmitted from the server device 40 (step S511), the colony detection determination unit 422 determines that the administrator instruction is a colony determination instruction and a colony correction. It is determined whether it is an instruction or a threshold change instruction (steps S512 and S513).

  At this time, if the colony detection determination unit 422 determines that the administrator instruction is a threshold change instruction, the colony detection determination unit 422 changes the corresponding threshold based on the threshold type and the value included in the threshold change instruction (step S514). ), The process proceeds to step S507.

  In addition, when the administrator instruction is determined to be a colony correction instruction, the colony detection determination unit 422 corrects the second colony to a non-colony (for example, the first colony based on the ID of the colony candidate included in the correction instruction). Correction to the first non-colony) or correction to the second non-colony colony (for example, correction to the first colony) is executed (step 515), and the process proceeds to step S508.

  Furthermore, when the administrator's instruction is determined to be a colony determination instruction, the colony detection determination unit 422 proceeds to the process of step S521 for colony determination and colony count.

  Next, the colony detection determination unit 422 counts the number of colonies as the number of colonies while confirming the first colony and the second colony as colonies (step S521), and the result (hereinafter, also referred to as “confirmed result”). It transmits to the administrator terminal device 20 (step S522).

  Next, the colony detection determination unit 422 reads out the medium determination criterion based on the type of the sample specified based on the work ID 71 and the fungus species to be detected, and detects the number of colonies detected based on the read sample determination criterion. It is determined whether or not satisfies a predetermined condition (step S523). That is, the colony detection determination unit 422 determines that it is acceptable (normal) when the predetermined condition is satisfied, and determines that it is unacceptable (abnormal) when the predetermined condition is not satisfied.

  Finally, the registration processing unit 421 registers the information on each colony candidate including whether or not it is a colony, the number of counted colonies, and the determination result of the medium determination in the medium information DB 401 in association with the medium ID 66 (Step S41). 524), this operation is terminated.

  On the other hand, when the administrator terminal device 20 receives the identified colonies and the determination result of the number of colonies (step S421), the administrator terminal device 20 displays the information so as to be viewable to the administrator (step S422) and ends the operation.

[7] Modification [7.1] Modification 1
In the above-described embodiment, the culture medium information registration process and the colony detection determination process are executed at the same timing, but the colony detection determination process may be executed at an arbitrary timing.

[7.2] Modification 2
In this embodiment, instead of performing the culture information registration process using a portable communication terminal device 10 such as a tablet information terminal device, a smartphone, or a mobile phone, a laptop or desktop personal computer is used. The registration process of the culture medium information may be realized by a computer and an image input device such as a scanner, a digital camera, or a smartphone.

  In this case, the personal computer and the image input device may be connected according to a predetermined communication standard, and the registration process of the culture medium information may be realized by using the personal computer and the image input device integrally, or a memory card. The medium image data and instruction sheet image data previously acquired by the image input device are stored in other physical memory, and the registration process of the medium information is realized while the stored image data is captured by the personal computer. May be.

[7.3] Modification 3
In the present embodiment, the portable communication terminal device 10, the administrator terminal device 20, and the server device 40 may be installed or used in the same site, or each may be installed in a remote place such as outside the country or remotely. It may be used on the ground and each process described above may be executed. However, it is assumed that the portable communication terminal device 10 is used in the same lot.

[7.4] Modification 4
In the present embodiment, registration of medium information in a specimen cultured in the film-type medium 60 using the film-type medium 60 is executed, but the medium in the specimen cultured in the agar medium using an agar medium. Information registration may be executed.

[7.5] Modification 5
In the present embodiment, instead of obtaining the culture medium ID 66 by imaging the film-type culture medium 60 by the image data generation unit 110, the culture medium ID 66 may be input by an operator's operation input.

  In this case, the application control unit 120 interlocks with the display control unit 161 and the operation unit 170 and causes the operator to manually input the culture medium ID 66 directly using the operation unit 170.

  In addition, the application control unit 120 controls the display control unit 161 and the operation unit 170 to select a display item such as a pull-down menu by clicking or touch selection, and causes the operator to input the culture medium ID 66.

[7.6] Modification 6
In the present embodiment, various processes are executed by the single server device 40, but various processes may be executed by a server system including a plurality of server devices 40.

[7.7] Modification 7
In the present embodiment, the colony detection determination unit 422 calculates the roundness based on the ratio between the distance to the shortest outer pixel and the distance to the longest outer pixel of the centroid coordinates of the colony candidate. However, it is also possible to use an index that increases the roundness when the concave / convex value (curvature) of the outer periphery of the colony candidate is calculated and the concave portion is small.

[7.8] Modification 8
In the present embodiment, the colony detection determination unit 422 uses the average feature amount or the average edge strength when calculating the likelihood of a colony candidate. A deviation or a maximum value may be used, and each likelihood may be calculated by machine learning based on teacher data acquired in advance.

[7.9] Modification 9
In this embodiment, the colony detection determination unit 422 may change the characteristics of the colony candidate used for calculating the likelihood for each bacterial species using the determination criterion information, or the colony candidate for each bacterial species. The characteristics may be read out from the criterion information and used.

  In such a case, the colony detection determination unit 422 calculates using a likelihood function by a plurality of methods, adds the respective likelihoods, or calculates the maximum likelihood among the respective likelihoods. The evaluation function may be calculated by using it. Note that the colony detection determination unit 422 calculates the likelihood according to the distribution of the feature amount of each pixel, for example, based on a region where the feature amount density of each pixel constituting the medium image is high, as in the seventh modification. The method may be changed.

  And such other elements should just be what shows colony-likeness by a value, for example, colony size is included. Specifically, when using the colony size when calculating the likelihood of each colony candidate, the colony detection determination unit 422 is based on a predetermined average colony size or already registered medium information. The difference fd (Ai) between the learned average colony size (number of detection pixels or area of the area) and the colony size of each colony candidate (number of detection pixels or area of the area) is calculated according to (Equation 4). Then, the calculated size difference fd (Ai) is used as the likelihood of the colony candidate or a part of the likelihood.

  As in the present embodiment, “σ” is a predetermined coefficient, and “fg (Ai)” and “fd (Ai)” are colonies in the “i” -th (colony candidate index number (ID)). Shows the likelihood based on other elements or colony sizes of candidates. “E (s)” represents an average value in the colony size, and “S (Ai)” represents a colony size in the “i” -th colony candidate. Further, (Expression 11) is an expression in which fd (Ai) approaches “1” if the difference between the average colony size and the colony size in the corresponding colony candidate is small, and approaches “0” if it is large.

[7.10] Modification 10
In the present embodiment, the imaging data generation unit 423 generates image data by distinguishing the display mode of the second colony and the display mode of the second non-colony, but the image data is not distinguished. It may be generated.

[7.11] Modification 11
In this embodiment, when there are many colony candidates to be corrected (that is, the second colony or the second non-colony), the imaging data generation unit 423 uses the colony selection threshold value, the colony erroneous detection confirmation threshold value, and the like. In addition, in order to reset the non-colony erroneous detection confirmation threshold, image data including a threshold adjustment bar that is linked to an operation unit (not shown) and a browsing function in the administrator terminal device 20 may be generated.

  In this case, the colony detection determination unit 422 selects the lonely and non-colony based on the colony selection threshold, the colony error detection confirmation threshold, and the Lonnie error detection confirmation threshold reset by the threshold adjustment bar. Or each discrimination | determination process is performed.

[7.12] Modification 12
In the present embodiment, the colony detection determination unit 422 executes ranking in at least one of the second colony and the second non-colony based on the calculated likelihood of each colony candidate, and forms an image. The data generation unit 423 may generate image data that changes the display mode such as pop-up display of the second colony or the second non-colony in order of likelihood closer to the colony selection threshold.

[7.13] Modification 13
In the present embodiment, the colony detection determination unit 422 binarizes the feature amount of each pixel such as luminance to detect colony candidates, but a portion different from the medium on the film-type medium 60 ( That is, if the colony candidate) can be detected, it is not limited to this method.

  For example, the colony detection determination unit 422 multiplies the feature amount of each pixel and, based on each pixel classified by a plurality of threshold values, determines a colony candidate based on the color change and the overlapping state at the position on the film type medium 60. Alternatively, colony candidates may be detected based on changes in the feature values of adjacent pixels with reference to an extreme value having a high feature value of each pixel.

  As described above, in the sanitary management system S of the present embodiment, the colony is determined according to a uniform standard by using the likelihood indicating the likelihood of colony from the colony candidates detected based on the feature amount of the pixels constituting the medium image. Therefore, it is possible to discriminate between colonies and non-colonys that may be erroneously detected (that is, colonies with a likelihood close to a non-colony and non-colony with a likelihood close to a colony). At the same time, the display mode of the colony or non-colony having a high possibility of erroneous detection can be changed to other colonies or non-colony, for example, highlighting.

  Therefore, in the sanitary management system S of the present embodiment, unlike the other colonies or non-colony, the administrator can easily watch the colony or non-colony that is likely to be erroneously detected. In addition, the confirmation work for confirming the status of each medium can be facilitated, and the workload of the administrator can be reduced. As a result, the colonies are accurately identified, and the medium information including them is accurately registered. be able to.

  In the sanitary management system S of the present embodiment, the description has been made using food. However, the present invention is not limited to the food, and can be applied to products that are provided to all living organisms including drugs and drugs. .

DESCRIPTION OF SYMBOLS S ... Hygiene management system 10 ... Portable communication terminal device 20 ... Manager terminal device 30 ... Network 40 ... Server device 60 ... Film type culture medium 70 ... Work instruction sheet 80 ... Work line 100 ... Data storage part 101 ... Application storage part 102 Image data storage unit 110 Image data generation unit 120 Application control unit 130 Network communication unit 140 Short-range wireless communication interface 150 Current position detection unit 160 Display unit 170 Operation unit 180 Timer 190 Mobile device management Control unit 400 ... database 401 ... medium information DB
402 ... Line information DB
403 ... Lot information DB
404 ... Work management DB
410 ... Communication control unit 420 ... Data processing unit 421 ... Registration processing unit 422 ... Colony detection determination unit 423 ... Imaging data generation unit 424 ... Lot determination processing unit 425 ... Report processing unit 430 ... Server management control unit

Claims (13)

An acquisition means for acquiring medium image data obtained by imaging the medium and imaging it as medium image data;
Detection means for detecting a colony candidate as a colony candidate based on a feature amount relating to the color of each pixel constituting the medium image of the acquired medium image data;
For each detected colony candidate, a calculation unit that calculates the likelihood of each colony candidate based on the characteristics of the colony candidate determined by the feature amount relating to the color of the pixel;
Sorting means for sorting the detected colony candidate into the colony and the non-colony based on the calculated likelihood,
Discriminating means for discriminating at least one of the selected colony and the selected non-colony into two or more types based on the calculated likelihood,
Providing display data for generating image data for imaging the determination result indicating the determined result on the medium image in a predetermined display mode, and for causing the administrator to view the generated image data Means,
Based on the determination result, registration means for registering information on the medium containing information on the colonies in the database as medium information;
A culture medium information registration system comprising: In the culture medium information registration system according to claim 1,
The discrimination means is
(1) The calculated likelihood of each colony is compared with a predetermined first threshold, and the first colony and second non-colony are closer to the non-colony than the first colony. A first determination process for determining a colony; and
(2) The calculated likelihood of each non-colony is compared with a predetermined second threshold, and the likelihood of the colony from the first non-colony and the first non-colony is determined from the non-colony. A second discrimination process for discriminating between the second non-colony
The land information registration system which performs at least any one of these and discriminate | determines at least any one of the said colony and the said non-colony into two or more types based on the said calculated likelihood. In the culture medium information registration system according to claim 2,
The providing means comprises:
Image data in which the display mode of the first colony and the second colony is different, Image data in which the display mode of the first non-colony and the second non-colony is different, and the colony and the non-colony A land information registration system that generates image data of at least one of image data having different display modes. In the culture medium information registration system according to claim 2,
Based on the instructions of the administrator, further comprises a setting means for performing setting of parameters used when performing the selection or the determination or resetting of the parameters,
The culture medium information registration system, wherein the parameter includes at least one of a selection threshold used when selecting the colony and the non-colony, the first threshold, and the second threshold. In the culture medium information registration system according to claim 4,
A culture medium information registration system in which at least one of the selection unit and the determination unit executes a selection or determination process based on the reset threshold for selection, the first threshold, or the second threshold. In the culture medium information registration system according to any one of claims 1 to 3,
The determining means performs ranking in at least one of the second colony and the second non-colony based on the calculated likelihoods,
The culture medium information registration system in which the providing means generates image data in which the display mode of the second colony or the second non-colony is changed based on the ranking. In the culture medium information registration system according to any one of claims 1 to 6,
A culture medium information registration system further comprising correction means for receiving correction of the determination result based on an instruction from the manager. In the culture medium information registration system according to any one of claims 1 to 7,
When calculating the likelihood of each colony candidate, when the detected colony candidates are imaged, the feature amount relating to the color of each pixel constituting the image or the arrangement position of each pixel on the medium image A medium information registration system in which at least one of them is used. In the culture medium information registration system according to any one of claims 1 to 8,
The registered medium information includes, for each colony, together with the number of colonies, a feature quantity relating to the color of each pixel constituting the image when the specified colony is imaged, and coordinates on the medium of each pixel. A culture medium information registration system including at least one of a position and the number of each pixel. Computer
An acquisition means for acquiring the culture medium image data obtained by imaging the culture medium as culture medium image data,
Detection means for detecting a colony candidate as a colony candidate based on a feature amount relating to a color of each pixel constituting the medium image of the obtained medium image data;
Calculating means for calculating the likelihood of each colony candidate based on the characteristics of the colony candidate determined by the feature amount relating to the color of the pixel for each detected colony candidate;
Sorting means for sorting the detected colony candidate into the colony and the non-colony based on the calculated likelihood,
Discriminating means for discriminating at least one of the selected colony and the selected non-colony into two or more types based on the calculated likelihood,
Providing display data for generating image data for imaging the determination result indicating the determined result on the medium image in a predetermined display mode, and for causing the administrator to view the generated image data Means and
Based on the determination result, registration means for registering information about the medium including information about the colonies in the database as medium information,
A program characterized by functioning as An acquisition means for acquiring medium image data obtained by imaging the medium and imaging it as medium image data;
Detection means for detecting a colony candidate as a colony candidate based on a feature amount relating to the color of each pixel constituting the medium image of the acquired medium image data;
For each detected colony candidate, a calculation unit that calculates the likelihood of each colony candidate based on the characteristics of the colony candidate determined by the feature amount relating to the color of the pixel;
Sorting means for sorting the detected colony candidate into the colony and the non-colony based on the calculated likelihood,
Discriminating means for discriminating at least one of the selected colony and the selected non-colony into two or more types based on the calculated likelihood,
Providing display data for generating image data for imaging the determination result indicating the determined result on the medium image in a predetermined display mode, and for causing the administrator to view the generated image data Means,
A medium image analysis apparatus comprising: Computer
An acquisition means for acquiring the culture medium image data obtained by imaging the culture medium as culture medium image data,
Detection means for detecting a colony candidate as a colony candidate based on a feature amount relating to a color of each pixel constituting the medium image of the obtained medium image data;
Calculating means for calculating the likelihood of each colony candidate based on the characteristics of the colony candidate determined by the feature amount relating to the color of the pixel for each detected colony candidate;
Sorting means for sorting the detected colony candidate into the colony and the non-colony based on the calculated likelihood,
Discriminating means for discriminating at least one of the selected colonies and the selected non-colonys into two or more types based on the calculated likelihood, and
Providing display data for generating image data for imaging the determination result indicating the determined result on the medium image in a predetermined display mode, and for causing the administrator to view the generated image data means,
A program characterized by functioning as A communication terminal device that generates a medium image obtained by imaging a medium as medium image data;
A server device for detecting colonies generated in the medium based on the medium image data and registering them in a database;
With
The server device is
An acquisition means for acquiring medium image data obtained by imaging the medium and imaging it as medium image data;
Detection means for detecting a colony candidate as a colony candidate based on a feature amount relating to the color of each pixel constituting the medium image of the acquired medium image data;
For each detected colony candidate, a calculation unit that calculates the likelihood of each colony candidate based on the characteristics of the colony candidate determined by the feature amount relating to the color of the pixel;
Sorting means for sorting the detected colony candidate into the colony and the non-colony based on the calculated likelihood,
Discriminating means for discriminating at least one of the selected colony and the selected non-colony into two or more types based on the calculated likelihood,
Providing display data for generating image data for imaging the determination result indicating the determined result on the medium image in a predetermined display mode, and for causing the administrator to view the generated image data Means,
Based on the determination result, registration means for registering information on the medium containing information on the colonies in the database as medium information;
Hygiene management system characterized by comprising.

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