JP2004189662A - Angiotensin converting enzyme inhibitor and antihypertension food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To develop an angiotensin converting enzyme inhibitor and an antihypertension food having a mild effects and allowing continuous intake. <P>SOLUTION: The angiotensin converting enzyme inhibitor and the antihypertension food, having activities for preventing or ameliorating hypertension are obtained by formulating one or more kinds selected from a shell of a seed of Jatoba (Hymenaea courbaril), a leaf of guava, Murraya koenigii, lagerstroemia speciosa, a leaf of Morus Bombycis, Tamarix chinensis Lour, Agrimonia pilosa, an extract of Mimusops elengi, conchiolin and a product obtained by treating a hydrolyzed product of the conshiolin with succinic anhydride. <P>COPYRIGHT: (C)2004,JPO&NCIPI

Description

【００４６】
実施例をそれぞれ血圧の高い人１０人づつに６ヶ月間使用してもらい使用前と使用後に血圧を測定して最高血圧の低下を見た。（１日当り、錠剤は１０錠、飲料は１００ｍｌ飲食してもらった）
以下にその結果を示す。（１０名の平均値である。）

【００４７】
【効果】上記のようにアンギオテンシン変換酵素を阻害し、実施例を飲食すると、血圧が低下し、有用であることがわかった。なお、副作用はなく安全性も高いことがわかった。[0001]
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an angiotensin converting enzyme inhibitor and an antihypertensive food which moderately lowers blood pressure.
[0002]
2. Description of the Related Art In recent years, lifestyle-related diseases have increased due to changes in dietary habits. Among them, hypertension is the most common disease. When hypertension occurs, angiotensin 2 is a substance that increases blood pressure in the body. This angiotensin 2 is made from angiotensin 1 by angiotensin converting enzyme which is present in lungs, vascular endothelial cells and renal proximal tubules. Inhibiting this enzyme can prevent hypertension. Angiotensin converting enzyme inhibitors such as captopril, cilazapril, and enalapril are known.
However, there was a night cough as a side effect, and the incidence of side effects was close to 20%, and eventually the continuation of this drug had to be interrupted due to this side effect.
[0003]
Jatoba (Jatoba) is a Takagi tree belonging to the genus Oochoenma of the leguminous family, and is called the Japanese name Ooyamae bean, scientific name Hymenaea courbaril. Distributed in tropical America, the height is 15 to 40 m, the diameter of the trunk is 100 to 200 cm, and the bark is light gray to gray. The wood is used for furniture and the like as wood, and the bark, resin, and endothelium are used for anthelmintic, healthy stomach, bronchitis, cystitis, urination, prostatitis, gonorrhea and the like. Beans are edible. It has already been disclosed to use an extract obtained by extracting Jatoba fruit or bark with water, ethanol or the like for whitening cosmetics (Japanese Patent Laid-Open No. 9-12441).
[0004]
Guava is called a banjirou or banshio tsumugi. It is a plant belonging to the genus Tungaceae, genus Vanjiro, and its scientific name is Psidium Guajava L. In general, the fruit is eaten with raw food, jam, juice or the like. Origin is tropical America, but it is widely cultivated in the tropics and subtropics, cultivated in southern Kyushu in Japan, and wild in the Ryukyu Islands. It is also used as a medicine for enteritis, dysentery and indigestive diarrhea.
[0005]
Murraya koenigii is a dicotyledonous net, a petal flower net, a diptera, and a citrus family plant that is distributed in India and Sri Lanka and is commonly found in dry lowlands. For medicinal purposes, it has been used for constipation, stomach pain, diarrhea, and the like.
[0006]
Lagerstroemia sp. Is a semi-deciduous tree that grows in India and is a plant belonging to the genus Sarsbergi. The roots of the giant clam are used for diarrhea and the bark and leaves are used as laxatives.
[0007]
Mulberry leaves are the dried leaves of mulberry trees whose scientific names are named Morus Bombycis, Morus alba and the like. Morse bombibisis is distributed throughout Japan, from the Minami Kuril, Korea, China, Vietnam to India, and from Kashmir to the temperate zone to the subtropics. A deciduous tree that grows in the mountains and is planted in a field, called mulberry or yamagua. Mors Alba is native to China and the Korean peninsula, and has come to Japan since ancient times. It is a deciduous tree that is planted in various places for garden trees, street trees, and sericulture, and is called Karagua and Touguwa.
[0008]
Nishikawayanagi is a young branch with leaves of a plant called Garyoridae, which has the scientific name of the genus Gyoryaceae, Tamarix chinensis Lour. Although this plant is originally from China, it is a small deciduous tree that came to Japan for viewing and was planted in gardens. Applications include sweating, antipyretic, diuretic, antitussive, antibacterial (pneumococcus, α-streptococcus, white staphylococci, influenza).
[0009]
The plant belonging to the genus Kimihiki belongs to the dicotyledonous network, the petal flower net, and the rose family. In Japan, Akimonia eupatoria grows naturally in Honshu, Shikoku, Kyushu, and Ryukyu, and is a perennial that grows on the mountains and roadsides. Outside Japan, it is distributed in the warm to temperate zones of Korea, Taiwan, Siberia and the Himalayas. This grass is called Senzaku grass and the whole grass is used. Its purpose is hemostasis, antistasis, anti-inflammation, tonic, and it is used for various bleeding symptoms such as vomiting, blood, stool blood, urinary blood, cerebral hemorrhage, and dysentery. However, as cosmetics, they are only used as head cosmetics in JP-A-1-313414 and as bactericidal compositions in JP-A-60-184024.
[0010]
Mimusops elengi is an evergreen small tree of the Acetaceae family from India to the Tarai region of Nepal, and is often planted as a garden tree. The bark is dark gray and cracked. The leaves are oval to oblong, and the flowers are fertile and single, or bunch are small, pale yellow and star-shaped, and have a good fragrance. For chemical use, the bark is astringent and tonic, and is also prescribed for heat. The leaves are also used for treatment when bitten by a snake. Ripe fruit pulp is astringent and is used to treat chronic diarrhea. Seeds are used for bruising, and in some regions they are used for enema during constipation in children. No patent has been filed for these uses.
[0011]
This is because conchiolin or a hydrolyzate thereof (referred to collectively as conchiolin) is excellent in moisture retention. JP-A-4-36214 discloses that conchiolin is effective as an antioxidant.
[0012]
In addition, the product obtained by treating this conchiolin hydrolyzate with succinic anhydride has increased water solubility even when the degree of hydrolysis is small, that is, the molecular weight is large, and the moisture retention is further improved than the conchiolin hydrolyzate. This is disclosed by the present inventors in Japanese Patent Application No. 5-312222.
[0013]
[Problems to be solved by the invention]
In view of the current situation as described above, the present inventors have developed an angiotensin converting enzyme inhibitor and an antihypertensive food that can be continuously ingested with moderate effects.
[0014]
[Means for Solving the Problems]
As a result of diligent studies to solve the above problems, the present inventors have extracted Jatoba (Hymenaea courbaril) husks, guava leaves, Muraya coenji, Greater crape myrtle, mulberry leaves, Nishikawa Yanagi, Sentsukusa, Misakinohana. It has been found that the above problem can be solved by using a product obtained by treating a product, conchiolin, or a hydrolyzate of conchiolin with succinic anhydride.
[0015]
As a method of the extract of Jatoba (Hymenea courbaril) husk, guava leaf, Muraya coenji, Greater crape myrtle, mulberry tea, Nishikawayanagi, Sentsukusa, Misakinohana used in the present invention, water or a hydrophilic organic solvent, For example, extraction is performed with ethanol, methanol, acetone or the like. However, since it is extraction of the raw material applied to a human body, naturally extraction with water, ethanol, or these mixed solvents is preferable. In some cases, polyhydric alcohols such as glycerin, 1,3-butylene glycol, and propylene glycol, or a mixture of polyhydric alcohol and water can also be used for extraction. Furthermore, lyophilization and use as a powder is also effective depending on the method of use.
[0016]
Conchiolin is a kind of hard protein contained in shells and pearls, and is relatively contained in pearl oysters, mussels and crows. Production methods are described in Japanese Patent No. 1587730, Japanese Patent No. 1560610, Japanese Patent No. 1587734, and the like.
[0017]
In the production method, a protein is obtained by decalcification from shells such as pearl oysters, mussels and mussels, and this is subjected to necessary hydrolysis by a usual method. One example is shown below, but is not limited thereto. Shells such as pearl oysters, mussels, and mussels are crushed and calcium content is removed with dilute hydrochloric acid, etc., and insoluble materials are collected by solid-liquid separation methods such as centrifugation and filtration decantation. Collect insoluble material by centrifugation, filtration, etc. Repeat as necessary.
[0018]
Furthermore, 2-10% hydrochloric acid aqueous solution may be added to this conchiolin and hydrolyzed at 50-110 ° C. for 5 hours to 5 days to obtain a conchiolin hydrolyzate. This is sometimes simply called conchiolin.
[0019]
Hydrolysis using dilute sulfuric acid instead of dilute hydrochloric acid, neutralization using barium hydroxide and neutralization to pH 5-6 using an alkali hydroxide solution, followed by centrifugation and filtration of the precipitate The hydrolyzed solution removed by the above method is made into a concentrated solution or a dry powder by using a known concentration method or drying method. In the hydrolysis, it goes without saying that the acid concentration, temperature and time should be controlled so that conchiolin does not completely decompose into amino acids.
[0020]
A method for preparing a substance obtained by treating a hydrolyzate of conchiolin with succinic anhydride is to add succinic anhydride while maintaining the pH at 6 to 9 while stirring in an aqueous solution of the hydrolyzate of conchiolin. The amount of succinic anhydride is adjusted depending on the purpose and the like because the amount added does not need to be succinylated or the rate of hydrolysis of conchiolin.
[0021]
Furthermore, the preparation method of the hydrolyzate of conchiolin treated with succinic acid is a cosmetic product with a new utilization method by adding the succinic anhydride to the substance prepared by the above method and stirring it while maintaining the pH at 6-9. Raw material is made. Since this method can be carried out regardless of the method of reducing the molecular weight, various methods including the usual acid method can be adopted for the degradation of conchiolin.
[0022]
Jatba (Hymenaea courbaril) husk, guava leaf, Muraya coenji, giant crape myrtle, mulberry tea, saiheyanagi, sensakusa, misaki nohana extract, conchiolin, conchiolin hydrolyzate treated as above An angiotensin converting enzyme inhibitor and an antihypertensive food are prepared by blending at least one kind of acid-treated pearl oyster glycoprotein. The dosage form is not particularly limited, and can be any form such as tablets, powders, liquids, tablets, powders, granules, dragees, capsules, suspensions, ampoules, injections, carriers, excipients, binders, dilutions. Prepare by mixing with the agent. In addition, beverages such as soup, fruit juice, vegetable juice, whey beverage, soft drink, ice cream, gum, candy, rice cake, Western confectionery, Japanese confectionery, jam, marmalade, cheese, butter, yogurt, etc. It can be used as food and drink by mixing with the form or other food and drink.
[0023]
EXAMPLES The production examples used in the present invention and examples which are actual utilization methods are described below, but the present invention is not limited by these production examples and examples.
[0024]
Production Example 1
500 g of pearls are gradually added with stirring 1 kg of hydrochloric acid to deash. Further, 300 g of 1N hydrochloric acid was gradually added while stirring to deash, and this was filtered to collect insoluble matters. 100 ml of sulfuric acid diluted 30 times with water was added thereto, sealed in a glass container, and thermally decomposed at 110 ° C. for 24 hours. After cooling, 90% barium hydroxide equivalent to the sulfuric acid used was added in small portions with good stirring. Further, 1% aqueous sodium hydroxide solution was added until pH 5.8. Next, it was centrifuged at 200 G for 10 minutes and then filtered through a 0.45 micron membrane filter to obtain a decomposition product. This was lyophilized. Yield was 8.9 g.
[0025]
Production Example 2
5.0 g of Production Example 1 was taken and dispersed in 100 ml of purified water. While stirring, 1 g of succinic anhydride was added 5 times every 15 minutes while maintaining 1-6 aqueous sodium hydroxide at pH 6-9. This was lyophilized. Yield was 9.9 g.
[0026]
Production Example 3
Jatoba JATOBA fruit (dried product) was added to 10 g of a 50% aqueous ethanol solution (300 ml) and allowed to stand for 5 days with occasional stirring. This was filtered and evaporated and then lyophilized.
[0027]
Production Example 4
300 g of 50% aqueous ethanol solution was added to 10 g of dried Senzaku grass and left for 5 days with occasional stirring. This was filtered and evaporated and then lyophilized.
[0028]
Production Example 5
The bark of dried Misakis elengi is added to 10 g of 300 ml of 50% aqueous methanol solution and heated with a reflux condenser. This was lyophilized.
[0029]
Production Example 6
To 10 g of guava leaf (dried product), 300 ml of 50% aqueous ethanol solution was added and left for 5 days with occasional stirring. This was filtered and freeze-dried.
[0030]
Production Example 7
Nishikawayanagi (dried product) was added to 10 g of 300 ml of 50% aqueous ethanol solution and allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0031]
Production Example 8
To 10 g of the leaf of the giant clam leaf (dried product), 300 ml of a 50% aqueous ethanol solution was added and allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0032]
Production Example 9
A branch (dry product) of Murara koenigii was added to 10 g of 300 ml of a 50% aqueous ethanol solution and allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0033]
Production Example 10
Mulberry (Morus alba) leaves (dried product) were added to 10 g of 300 ml of 50% aqueous ethanol solution and allowed to stand for 5 days with occasional stirring. This was filtered and freeze-dried.
[0034]
Example-1 Tablet (parts by weight)
Production Example 1 10.0
Lactose 30.0
Cornstarch 26.0
Crystalline cellulose 30.0
Polyvinylpyrrolidone 3.0
Mg stearate 1.0
According to a conventional method, each of the above components was granulated and compression-molded to prepare 1000 tablets of 500 mg tablets.
[0035]
Example-2 Beverage Production Example 2 1g
Xylitol 10g
Vitamin B1 hydrochloride 0.5mg
Vitamin B2 0.2mg
Vitamin C 500mg
Niacin 1.0mg
Pantothenic acid Ca 1.0mg
100 g of purified water
According to a conventional method, each of the above components was measured and mixed.
[0036]
Example-3
The production example 1 of Example-1 was replaced with the production example 3, and others were produced similarly to Example-1.
[0037]
Example-4
Production Example 1 of Example-2 was replaced with Production Example 4, and the others were produced in the same manner as Example-2.
[0038]
Example-5
The production example 1 of Example-1 was replaced with the production example 5, and others were produced similarly to Example-1.
[0039]
Example-6
Production Example 1 of Example-2 was replaced with Production Example 6, and the others were produced in the same manner as Example-2.
[0040]
Example-7
Production Example 1 of Example-1 was replaced with Production Example 7, and the others were produced in the same manner as Example-1.
[0041]
Example-8
Production Example 1 of Example-2 was changed to Production Example 8, and the others were produced in the same manner as Example-2.
[0042]
Example-9
Production Example 1 of Example-1 was replaced with Production Example 9, and the others were produced in the same manner as Example-1.
[0043]
Example-10
Production Example 1 of Example-2 was replaced with Production Example 10, and the others were produced in the same manner as Example-2.
[0044]
The test for which the effectiveness has been confirmed will be described below.
Angiotensin-converting enzyme inhibition test test method Add 0.25 ml of 100 mM HEPES buffer (pH 8.3) containing 5 mM Hipriluhistidylleucine to 0.5 ml of 1 mg / ml sample solution and incubate at 37 ° C. for 3 minutes. Then, 0.25 ml of 3.0 mU / ml angiotensin-1 converting enzyme (ACE) was added and reacted at 37 ° C. for 30 minutes. After adding 2.0 ml of 0.1N sodium hydroxide solution to stop the reaction, 0.1 ml of 0.2% o-phthalaldehyde methanol solution was added to form a free fluorescent derivative of histileuleucine. And left at 4 ° C. for 15 minutes. After neutralizing the reaction solution by adding 0.4 ml of 1.5 M phosphoric acid solution, the fluorescence intensity (C) at an excitation wavelength of 360 nm and a fluorescence wavelength of 480 nm was measured. The same operation was performed for the sample not containing the sample (A), the sample not containing the ACE (B), and the sample not containing the ACE (D). The rate, ie ACE inhibition rate, was calculated.
Inhibition rate (%) = {1− (C−D) / (A−B)} × 100
[0045]
The results are shown below.

[0046]
Each of the ten examples with high blood pressure was used for 6 months, and blood pressure was measured before and after use to see a decrease in the maximum blood pressure. (10 tablets per day and 100 ml of drinks per day)
The results are shown below. (The average value of 10 people.)

[0047]
[Effect] It was found that when angiotensin converting enzyme was inhibited as described above and the examples were consumed, blood pressure was lowered and useful. It was found that there were no side effects and safety was high.

Claims (1)

1 of Jatoba (Hymenaea courbaril) husk, guava leaf, Muraya coenji, giant crape myrtle, mulberry leaf, Nishikawayanagi, Sentsukaku, Misakinohana extract, conchiolin, conchiolin hydrolyzate treated with succinic anhydride Angiotensin-converting enzyme inhibitor and antihypertensive food, which contain more than one species and have an effect of preventing and improving hypertension