KR100768830B1 - Extract of echinosophora koreensis removing hangover and having anti-oxidant activity - Google Patents

Abstract

A hangover reducing composition containing Echinosophora koreensis extract as an active ingredient is provided to be used as a hangover reducing agent by increasing the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase. A hangover reducing composition contains 0.0001 to 50% by weight of Echinosophora koreensis extract and additionally various flavorings or natural carbohydrates such as monosaccharide, disaccharide, polysaccharide and sugar alcohols. The Echinosophora koreensis extract is obtained by reflux-extracting Echinosophora koreensis with water, C1-3 lower alcohol or a mixed solvent thereof for 2 to 4hr. The mixed solvent contains water and lower alcohol in a weight ratio of 2:8.

Description

Extract of Echinosophora koreensis removing hangover and having anti-oxidant activity

1 is a view showing the effect on the radical scavenging ability of the extract of Echinacea,

2 is a view showing the effect of changing the activity of alcohol dehydrogenase of Echinosophora koreensis extract,

3 is a view showing the effect of changing the activity on acetaldehyde dehydrogenase of Echinacea extract,

4 is a view showing the alcohol metabolism promoting effect of the extract of Echinacea.

The present invention relates to an extract of Echinosophora japonica having a hangover relief and antioxidant activity.

A hangover is a headache, diarrhea, anorexia, nausea, vomiting, chills, or cold sweating after drinking alcohol. Objective symptoms include perception, decreased motor performance, hematologic changes, and hormonal changes. The causes of hangovers are known as dehydration, toxicity of alcohols and alcohol metabolites (acetaldehyde, formaldehyde, acetone, etc.), and nutrient deficiencies due to malabsorption (lack of blood sugar, vitamins, minerals). The degree of hangover is very different depending on the individual's genetic variation, environmental conditions (nutrition, exercise, dehydration, health).

In Korea, consumption of alcohol has surged along with economic growth since the 1980s. In 1996, the average daily drinking volume per adult was six bottles of beer (Chosun Ilbo, a change in drinking culture). Alcohol acts on the central nervous system of the brain to make you feel better and forget about the pain. In ancient times, alcohol was used as a basic excipient for all drugs, and since then, it is gradually taking a large part in socializing. In Korea's drinking culture, many people are showing high interest in drugs or beverages that can get rid of hangovers due to heavy drinking and frequent drinking. The hangover that appears after drinking alcohol itself is not only toxic but also converts into harmful substances in the body during metabolic processes in the body, which is a symptom caused by harmful substances to the digestive system including the brain and liver.

After drinking alcohol is absorbed in small amounts in the esophagus and oral mucosa, about 10% in the stomach 90% in the small intestine. Alcohol is metabolized through three pathways. When the concentration of ethanol is low, alcohol dehydrogenase and acetaldehyde dehydrogenase are present in the gastrointestinal tract or liver. It is metabolized to acetaldehyde and acetic acid by the microsomal ethanol oxidizing system (MEOS) present, and then through the action of catalase present in the peroxisome, carbon dioxide (CO ₂ ) And decomposed into water (H ₂ O). When the proper amount of alcohol is introduced, the metabolic system described above works smoothly and does not cause any symptoms caused by alcohol. However, when a large amount of alcohol is introduced, the balance of the metabolic system is destroyed and thus the homeostasis cannot be maintained. Under these circumstances, the most affected organs are the liver. In the long term, various liver dysfunctions such as fatty liver and cirrhosis occur, and short-term symptoms include headache or double head, loss of concentration, heartburn and indigestion.

In order to relieve hangovers, bean sprouts soup and northern fish soup have been used since ancient times. In particular, the bean sprouts root contains abundant aspartic acid known to protect the liver by promoting the action of alcohol dehydrogenase and promoting alcohol decomposition. The early hangover drinks were made and sold with aspartic acid as the main ingredient.

Currently, hangover drinks are available from Jagi, Astragalus, Lotus seed, rice germ, etc. (CJ Co., Ltd.), Dimkyu (Ceun Kun Dang) based on high-purity chitosan, Dawn tree, and rowan extract 808 ( Dozens of papers are sold, and research publications and patents have been filed. In particular, as the results of comparing the functions of each product are now available for propaganda and advertisement, changes in blood ethanol and acetaldehyde concentrations in animals or humans, and activity of enzymes related to blood liver function (ALT, AST, ALP, etc.) Simple clinical studies on sex changes, hangover-related medical questions, etc. are conducted by each company individually. Materials of the earth, neck, fruit extract, persimmon extract, green tea extract, ginseng (red ginseng) extract, etc. are being studied.

Most of the research on hangover is done through animal experiments in Korea. Japan is focusing on the development of materials to relieve hangovers, while the United States and Europe are actively engaged in research on the symptoms of poisoning of ethanol (methanol), toxic agents and toxic mechanisms. In particular, the structure of tetrahydroisoquinoline or tetrahydro-beta-carbolines produced by the metabolism of ethanol is similar to morphine, resulting in withdrawal symptoms, leading to ethanol dependence. do. This is known to be due to a decrease in ethanol metabolizing enzymes and in part to a decrease in the response of brain neurons. Recently, the metabolism of methanol and formaldehyde in small amount of alcohol (ethanol) has been pointed out as a cause of hangover, and the actual alcohol hangover is reported to be due to alcohol metabolism, acetaldehyde, acetone, etc. It is entering (Moser J, Bagchi D, Akubue PI, Stohs SJ .. Alcohol Alcohol . 28: 287-295, 1993). In Japan, there was a patent claim that fermentation products of rice bran extract contributed more to lower acetaldehyde concentration than blood alcohol concentration. There have been few reports (Wall TL, Horn SM, Johnson ML, Smith TL, Carr LG., J Stud . Alcohol . 61: 13-17, 2000), focusing on acetaldehyde as the causative agent of alcohol-induced hangovers. Are gathering.

Looking at the patents on the existing hangover, it is in the form of beverages, health functional foods, chewing gum, etc., and the raw materials used are silk fibroin (Korean Patent No. 0528740), Ogapi, Root, Chinese quince, cherry tree, low head river (Korea) Registered Patent No. 0528388), wormwood, goji berry, health, dermis, lotus root, earth, lettuce, Schisandra chinensis (Korean Registered Patent No. 0512912), astaxanthin, aspartic acid (Korean Registered Patent No. 0520985), persimmon leaf, persimmon (Korean Registered Patent No. 0504351), Dermis, Baekbokyeong, Injin, Galhwa, Kaleun (Korean Registered Patent No. 0500373), Asparagus, Green Tea, Onion, Plum (Korean Registered Patent No. 0496524), Dharm (Korean Registered Patent No. 0462329 No.), soybeans, bean sprouts, grape seeds (Korean Patent No. 0455075), cricket (Korean Patent No. 0453475), active ingredient Hobenofinolol (Korean Patent No. 0452128), radish , 칡, persimmon leaf, three hundred seconds (Korea Patent No. 0451013), Deckersinol (Korea Patent No. 0448680), earth, earth tree, hut mixture, brown root, Baek Ha Shou, mountain medicine, peony (Korea Registered Patent No. 0446061), Baekmo-geun, Audi, Tosa, Gallium, Creation, Bokbunja, Snakeberry, Rhododendron, Gil-nucleus (Korean Registered Patent No. 0442771), Pine Needles (Republic of Korea Registered Patent No. 0412425), Bark Wood, etc. (Korea Registered Patent) 0403720-22), brown flower, thistle seed, taurine, turmeric (Korea Patent No. 0392876), Narinjin, Naringenin (Korea Patent No. 0375047), alder, rowan (Korea Patent No. 0181168) Etc. Raw materials used in other applied patents include horseshoe mushrooms, green tea catechins, ogapi, palm cactus (baeknyeoncho), cheonma, roe deer mushrooms, chodugu, propolis, jahwajak, falcon, sesame oil and the like. However, until now, the hangover, food, chewing gum and the like have been developed using the above materials, but the effect is almost outstanding.

All aerobic organisms, including humans, survive through energy metabolism using oxygen (O ₂ ). However, when oxygen in the body is subjected to various physical, chemical and biological stresses, harmful substances such as superoxide anion radical (O ₂ −), hydrogen peroxide (H ₂ O ₂ ) and hydroxy radicals (OH) It turns into active oxygen species, causing fatal physiological disorders in the body and, in severe cases, causing disease and losing life.

Although living organisms are thought to have evolved from the self-defense mechanism that removes reactive oxygen species, developing antioxidant mechanisms, the generation of reactive oxygen species that transcends the defenses of tissues is not immune to proteins, DNA, enzymes and T cells. Damage to the system factors cause various diseases. In addition, reactive oxygen species attack perunsaturated fatty acids, which are components of cellular membranes, to cause peroxidation reactions, and lipid peroxides accumulated in vivo are known to cause aging and various diseases (Halliwell, B.,Drugs 42: 569, 1999; Fukuzawa, K and Takaishi, Y.,J. Act . Dxyg . Free Rad . 1:55, 1990; Neuzil, J., Gebicki, J. M. and Stocker, R.,Biochem . J. 293: 601, 1993; Sies, H., ed.,Oxidative stress, 1985; Steinberg. D., et al.,N. Engl . J. Med . 320: 915. 1989).

Recently, as the theory that aging and geriatric diseases are caused by reactive oxygen species has been recognized, research on the development of antioxidants known as substances capable of regulating reactive oxygen species has been actively conducted, and superoxide dismutase Antioxidants such as oxidase, catalase, and glutathione peroxidase, antioxidant enzymes such as tocopherol, ascorbate, carotenoid, and glutathione Much research has been done on (Chang, SS, et al ., J. Food Sic . 42: 1102, 1977; Hammerschmidt, PA and Pratt, DE, J. Food Sic . 43: 556, 1964), synthetic antioxidants such as 2,6-di-tert-butyl-4-hydroxyanisole (BHA) have been developed. It is used in the food field (Kitahara, K., et al ., Chem Pharm . Bull . 40: 2208, 1992; Hatano, T., Natural Medicines 49: 357, 1995; Masaki, H., et al ., Biol. Pharm . Bull . 18: 162, 1995).

However, consumer avoidance of synthetic antioxidants and carcinogenicity have been reported in animal experiments in which large amounts of synthetic antioxidants are administered (Branen, AL, JAOCS 52:59, 1975). The use of synthetic antioxidants is becoming increasingly limited. As such, there is an urgent need to develop new natural antioxidants that are both effective and safer.

Echinosophora koreensis It is an outpost of the legumes nectarine. Also known as ant grass, red ginseng, cedar, and sticks of thieves. It is distributed in the north of Yang-gu, Gangwon-do, Pyeongannam-do, and Hamgyongnam-do. It is a deciduous shrub in the foothills or roadsides. The plant multiplies under the ground and its branches are dark brown and hairy. Winter snow is covered with fur and invisible. Leaves are pinnate, leaves are 13-25 sheets, leaf surface is white, hairy, long oval shape, blunt, hairy edge. Chin leaves do not fall into thorns. In May, yellow flowers bloom in turn. Inflorescences are 3-5 cm long, hanging from the end of a new branch, with a flower about 15 mm in diameter. Small vesicles are basso, black, with hairs. The calyx is divided into 5 pieces and the rear two are slightly smaller. Contains alkaloids and flavonoids. Current antimicrobial effect (Sohn, H.-Y. et al ., Phytomedicine . 11: 666-672, 2004) and antimicrobial effects on MRSA (Hironori T et al ., J. Ethnopharmacol . 5027-5034, 1996).

The present inventors confirmed that the extract of Echinosophora japonica from the plants growing in the high cold region showed excellent radical scavenging ability, and increased the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase to reduce alcohol alcohol and acetaldehyde concentration by promoting alcohol metabolism in vivo. . In addition, the hangover can be removed more efficiently than a commercially available hangover-removing drink and confirmed that there are no side effects, the present invention was completed.

It is an object of the present invention to provide novel hangovers and antioxidants comprising the extract of Echinacea.

In order to achieve the above object, the present invention provides an extract of Echinosophora chinensis, which is extracted with water, alcohol or a mixture thereof and has hangover and antioxidant activity.

The present invention also provides a hangover resolver comprising the extract of Echinosophora koreensis as an active ingredient.

In addition, the present invention provides a hangover for relieving health food comprising the extract of Echinacea as an active ingredient.

The present invention also provides an antioxidant comprising the extract of Echinacea as an active ingredient.

The present invention also provides an antioxidant health food comprising the extract of Echinacea as an active ingredient.

The present invention also provides a cosmetic for antioxidant comprising the extract of Echinacea as an active ingredient.

In addition, the present invention provides an aging inhibitor comprising the extract of Echinacea as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides an extract of Echinosophora japonica having a hangover relief and antioxidant activity.

The present inventors extracted the dried ginseng extract to investigate the effect of the extract of the Korean ginseng on hangover. The extract of Echinacea may be used by a general extraction method known in the art, and extracted by the following extraction method: 1) obtaining an extract from Echinacea using water, alcohol or a mixture thereof; 2) filtering the extract of step 1); 3) concentrating the filtrate of step 3) under reduced pressure. The alcohol in step 1) is preferably ethanol or methanol, the volume is preferably 5 to 10 times. The extraction time is preferably 2 to 24 hours, most preferably 3 hours.

Extract of Echinosophora japonica exhibits excellent radical scavenging activity (see Table 1 and FIG. 1) and increases the activity of alcohol dehydrogenase (see Tables 2 and 2) and acetaldehyde dehydrogenase (see Tables 3 and 3) in a concentration dependent manner. , When administered to experimental animals shows better alcohol decomposing ability than the commercial hangover drink in vivo (see Table 4 and Figure 4). In addition, the measurement of hangover relief for the general public reduced headache and gastrointestinal discomfort, which also appears to be more effective than commercial hangover drink (see Table 6). Through this, the extract of Echinacea promotes alcohol metabolism in vivo, effectively reducing blood alcohol concentration and acetaldehyde concentration, and removing hangover more effectively than commercially available hangover quenching drinks. It can be usefully used as a health food.

The present invention provides a hangover resolver comprising the extract of Echinacea as an active ingredient.

Hangsam extract having a hangover relief function of the present invention can be administered orally or parenterally during clinical administration, one or two or more pharmaceutically acceptable conventional carriers or one or two in an effective amount of the extract of the main extract More than one additive may be selected to prepare the compositions of conventional pharmaceutical formulations.

In the present invention, the carrier and the additive may be used in any pharmaceutically acceptable, specifically, as a diluent lactose (lactose monohydrate), corn starch (cornstarch), soybean oil (soybean oil), microcrystalline cellulose (microcrystalline cellulose) ) Or mannitol (D-mannitorl) is preferable, magnesium stearate or talc is preferred as a lubricant, polyvinyipyrolidone (PVP: polyvinyipyrolidone) or hydroxypropyl cellulose (HPC :) hydroxypropylcellulose). In addition, the disintegrant may be selected from among carboxymethylcellulose calcium (Ca-CMC), sodium starch glycolate, polyacrylin potassium or cross-linked polyvinylpyrrolidone. Preferably, the sweetening agent is selected from sucrose, fructose, sorbitol or aspartame, and as a stabilizer, carboxymethylcellulose sodium (Ma-CMC), beta-cyclodextrin, It is selected from white bee's wax or xanthan gum, and preservatives include methyl p-hydroxy benzoate (methhlparaben), propyl p-hydroxy benzoate (propylparaben), or sorbic acid. It is preferable to select from potassium sorbate.

Hangeul extract of the hangover function of the present invention is to be used for the purpose of efficient hangover removal by promoting alcohol metabolism in vivo, reducing blood alcohol concentration and acetaldehyde concentration is not particularly limited in the formulation, It can be used in beverages, powders, capsules, soft capsules, tablets, gums, adhesive type liquid compositions, and can be used in transdermal dosage forms such as lotions, ointments, gels, creams, patches, or sprays. It can be used in preparations such as powders and granules.

In vitro ( in vitro) and in vivo (in vivo), and the extract gaeneusam on the basis of experimental preferably 0.0001 to 50% by weight of the total weight of the composition for hangover comprising as an active ingredient, most preferably from 0.01 to 5% by weight.

Extract of Echinosophora sieved hangover function of the present invention may further contain one or more active ingredients exhibiting the same or similar functions.

In addition to the above-mentioned main components, the extract of the Korean ginseng extract having a hangover-relieving function according to the present invention contains minerals such as vitamins B, C, E, beta-carotene, Ca, Mg, and Zn, phospholipids such as lecithin or maltol, oligosaccharides, amino acids, etc. Compounds of the present invention can be used, and among them, the use of 2 to 3 components in vitamin C, E or beta-carotene, maltol, etc. is more preferable because it can enhance the biological activity effect.

In addition to the above components, in order to enhance the taste as a known additive, natural flavors such as plum, lemon, pineapple or herb, or natural fruit juice, natural pigments such as chlorophyllin and flavonoids, and fructose, sweet sugar Can be mixed with honey, sugar alcohol, sugar and acidulants such as citric acid and sodium citrate.

The present invention provides a hangover remedy health food comprising an extract of the Echinosophora japonica which has a function for relieving hangover.

Health foods containing the extract of Echinosophora japonica, which function to relieve hangover of the present invention, include folk remedies for the hangover, such as tea, jelly, juice, extract, and beverages, which contain the extract of Echinacea as an active ingredient. . The health food of the present invention processed in various forms as described above is excellent in detoxification without side effects to the human body, easy to take and can be stored for a long period of time can be usefully used to relieve hangovers before and after drinking.

Hangyeom extract of the hangover function of the present invention can be added to health food for the purpose of relieving hangover. When using the Echinacea extract of the present invention as a food additive, the Echinacea extract may be added as it is, or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, during the production of food or beverages, the extract of Echinosophora cava of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on 100 parts by weight of the raw material. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and there is no problem in terms of safety, so the active ingredient may be used in an amount above the above range.

There is no particular limitation on the kind of food. Examples of foods to which the extract of hangover ginseng may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinks , Teas, drinks, alcoholic beverages and vitamin complexes and the like, and includes all healthy foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors, natural carbohydrates, and the like as additional components, as in a conventional beverage. Natural carbohydrates described above are glucose, monosaccharides such as fructose, malsaccharides, disaccharides such as sucrose, and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin, aspartame, and the like can be used. The proportion of natural carbohydrates is generally from 0.01 to 0.04 g, preferably from about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

The composition of the present invention other than the above is a variety of nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention provides an antioxidant comprising the extract of Echinacea as an active ingredient.

Radicals generated in vivo cause fatal physiological disorders in the human body and, in severe cases, cause disease and cause life loss. Echinacea extract of the present invention shows excellent radical scavenging ability (see Table 1) and can be usefully used for anti-aging due to its excellent antioxidant activity.

The present invention provides an antioxidant health food comprising the extract of Echinosophora japonica having the antioxidant activity.

When using the Echinacea extract of the present invention as a food additive, the Echinacea extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient can be suitably determined according to the purpose of use (prevention, health or therapeutic treatment). In general, Echinacea extract of the present invention is added in the amount of 0.1 to 5 parts by weight, preferably 2 or 10 parts by weight based on 100 parts by weight of the raw material in the manufacture of food or beverage. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

There is no particular limitation on the kind of food. Examples of the food to which the substance may be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, ice cream, various soups, drinks, tea, drink, Alcoholic beverages and vitamin complexes, and the like and all of the health foods in the conventional sense.

The present invention provides an anti-oxidant cosmetic comprising the extract of Echinosophora chinensis which has the antioxidant activity.

Antioxidant cosmetics of the present invention include, but are not limited to, lotions, ointments, gels, creams, patches or sprays. In preparing an antioxidant cosmetic containing the extract of Echinacea extract of the present invention, the extract of Echinacea extract of the present invention may be added to 1 to 15 parts by weight, preferably 2 or 10 parts by weight, to the externally used skin composition. In addition to the extract of Echinacea, the skin external preparation of the present invention contains fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ions Any commonly used in type or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or external preparations for the skin It may contain supplements commonly used in the field of dermatology, such as other ingredients of. The ingredients may also be introduced in amounts generally used in the field of dermatology.

The present invention provides an aging inhibitor comprising the extract of Echinacea as an active ingredient.

Aging is caused by free radicals occurring in metabolic processes (Harman, D., Free radical theory of aging: Role of free radicals in the origination and evolution of life, Aging and Disease Processes, p.3-49, Alan R Liss , New York, 1986). Since the present invention possesses excellent antioxidant activity (see Table 1), it can be usefully used as an aging inhibitor.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples, Experimental Examples, and Preparation Examples.

< Example  1> with alcohol Cedar  Extract manufacturer

1 Kg of dried Echinacea starch was added to an extraction bag, immersed in 10% of 100% methanol, extracted under reflux for 3 hours, cooled to room temperature, and filtered through a filter paper. The extract was concentrated by using a concentrator at 50 ° C. under reduced pressure until the solution completely evaporated, thereby obtaining crystals.

As a result of using the above method, 68 g of the extract could be prepared from 1 kg of dried Echinacea.

< Example  2> using water Cedar  Preparation of Extract

1 Kg of dried Echinacea starch was placed in an extraction bag, immersed in 10 times of water, extracted with hot water at 100 ° C. for 90 minutes, cooled to room temperature, and filtered through a filter paper. The extract was concentrated by using a concentrator at 50 ° C. under reduced pressure until the solution completely evaporated, thereby obtaining crystals.

As a result of using the above method, 46 g of extract could be prepared from 1 kg of dried Echinacea starch.

< Example  3> using a mixture of water and alcohol Cedar  Preparation of Extract

1 Kg of dried Echinacea starch was added to an extraction bag, immersed in 10% of 80% methanol mixed with water and methanol at 2: 8, refluxed for 3 hours, cooled to room temperature, and filtered through a filter paper. The extract was concentrated by using a concentrator at 50 ° C. under reduced pressure until the solution completely evaporated, thereby obtaining crystals.

As a result of using the above method, 59 g of the extract could be prepared from 1 kg of dried Echinacea.

< Experimental Example  1> Radical Scavenging power  Measure

Antioxidant activity was measured by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method in order to confirm the antioxidant activity of the extract of Echinacea extract of Examples 1 to 3 prepared by the above method (Taco, T et al ., Biosci . Biotech . Biochem . 58: 1780-1783, 1994; Na, M. K et al ., Nat Prod . Sic . 8: 26-29, 2002). DPPH is a relatively stable free radical, which exhibits a maximum absorbance at 517 nm when present in the radical state, and loses absorbance when it is eliminated. Specifically, the extract of Echinacea was diluted with a 70% ethanol solution in 10 steps at a concentration of 500 μg / ml in a concentration of 500 μg / ml in 96 well plates, followed by DPPH (1,1-diphenyl-2) prepared as a 100 μM ethanol solution. -picryl-hydrazyl) was added to adjust the total volume of the solution to 200 μl. Thereafter, the reaction was measured at 37 ° C. for 30 minutes, and then the absorbance was measured using a 520 nm ELISA reader, and the sample was not added. ₅₀ (inhibitory Concentration) (Table 1 and Figure 1).

Cedar  Of extract Radical Scavenging power ( IC ₅₀ Unit μg / ml) IC ₅₀ (μg / ml) Example 1 10.62 Example 2 9.97 Example 3 10.27 Vitamin c 2.51 Trolox 3.14

In this experiment, vitamin C and trolox were used as positive controls. As a result, it was found that the extract of Echinosophora japonica exhibits excellent radical scavenging ability, which may be useful for the anti-aging effect of Echinacea extract of the present invention.

< Experimental Example  2> Alcohol dehydrogenase  Activity measurement ( in in vitro )

The enzyme source was 26.4 mg of rat liver homogenate (MOLTOX Co., USA) of lyophilized S9 rats (final concentration was 3.3 mg / ml) and 8 ml of 0.1% BSA (bovine serum albumin) solution. Dissolved in and filtered through a 0.45 μm syringe filter. Alcohol dehydrogenase activity was measured using the rate of NADH production as absorbance at 340 nm. The reaction mixture consisted of 1 ml of distilled water, 0.75 ml of 1 M tris-HCl (pH 8.8), 0.3 ml of 20 nM NAD +, 0.3 ml of ethanol, 0.1 ml of sample, and 0.15 ml of enzyme source in a cuvette. After the reaction was adjusted to mL for 5 minutes at 30 ° C., the change in absorbance was measured at 340 nm for 5 minutes. At this time, the sample was not added as a control. Alcohol dehydrogenase activity of the samples was determined as relative activity (%) relative to the control (Table 2 and FIG. 2).

Cedar  Of extract Alcohol dehydrogenase  Activity (% of unit, concentration μg / ml) 100 μg / ml 50 μg / ml 10 μg / ml Example 1 400 280 200 Example 2 350 250 150 Example 3 370 260 180

As a result, it can be seen that the extract of Echinosophora japonica extract increased alcohol dehydrogenase activity relatively better than the control group, and that the concentration of alcohol extract increased significantly.

< Experimental Example  3> Acetaldehyde dehydrogenase  Activity measurement ( in in vitro )

Acetaldehyde dehydrogenase activity was measured by the rate of NADH production at an absorbance of 340 nm. The composition of the reaction solution was cuvetted with 2 ml of distilled water, 0.3 ml of 1 M tris-HCl (pH 8.0), 20 mM NAD ⁺ 0.1 ml, 0.1 ml of 1 M acetaldehyde, 0.1 ml of sample and 0.1 ml of enzyme source. The reaction was adjusted to a total of 3 ml and reacted for 5 minutes at 30 ° C., and then the change in absorbance at 340 nm was measured for 5 minutes. In this case, the acetaldehyde dehydrogenase activity of the sample was measured as a relative activity (%) relative to the control group without the addition of the sample (Table 3 and Figure 3).

Cedar  Of extract Acetaldehyde dehydrogenase  Activity (% of unit, concentration μg / ml) 100 μg / ml  50 μg / ml 10 μg / ml Example 1 380 280 210 Example 2 350 250 180 Example 3 360 250 190

As a result of the experiment, it can be seen that the extract of Echinacea increased acetaldehyde dehydrogenase activity relatively better than the control group, and the activity of acetaldehyde dehydrogenase of the extract of Echinacea increased with increasing concentration.

< Experimental Example  4> Determination of ethanol resolution ( in vivo )

The experimental animals were magnetic Sprague Dawley rats weighing 200-250 g. The samples were used for pre-breeding after two weeks of free feeding of solid samples and tap water. After 18 hours of fasting, test samples were prepared orally in 0.5% CMC (Carboxy methyl cellulose) solution at a concentration of 500 mg / Kg. After 30 minutes, the blood was collected from the heart at 1, 3 and 5 hours after oral administration of alcohol at 3 g / Kg. The collected blood was mixed with perchloric acid in the same amount, centrifuged at 3,000 rpm for 10 minutes, and the supernatant was mixed with 0.5 volume of buffer solution I and left at 4 ° C. for 30 minutes. After removing proteins by centrifugation at 3,000 rpm for 10 minutes, the concentration of ethanol was measured using an ethanol measurement kit (Roche, USA) (Table 4 and FIG. 4).

Alcohol Degradation of Echinacea Extract (Unit g / ℓ) 1 hours 3 hours 5 hours Control -0.01 -0.01 -0.01 Ethanol group 0.06 0.07 0.067 Company C product 0.032 0.04 0.038 Company G 0.055 0.054 0.048 Example 1 500 mg / kg 0.03 0.028 0.025 Example 1 250 mg / kg 0.04 0.039 0.029 Example 2 500 mg / kg 0.031 0.03 0.029 Example 2 250 mg / kg 0.042 0.041 0.033 Example 3 500 mg / kg 0.03 0.029 0.027 Example 3 250 mg / kg 0.041 0.043 0.032

As a result of the experiment, it can be seen that the extract of Echinacea very well promotes blood alcohol metabolism, thereby reducing the amount of alcohol.

Experimental Example 5 Measurement of Hangover Relief Effect

In order to confirm the hangover relief effect of the composition of the present invention 5 to g and CMC 5 g of the extracts of Examples 1 to 3 were prepared by adjusting 1,000 ml with purified water. As shown in Table 5, Preparation Examples 1 to 3 and Comparative Examples 1 to 3 were prepared and divided into six groups of 60 healthy male adults in their 30s, and the hangover relief effect was measured.

Furtherance Echinacea Extract CMC Company C product Purified water Preparation Example 1 5 (Example 1) 5 - Fit to 1,000 ml Preparation Example 2 5 (Example 2) 5 - Fit to 1,000 ml Preparation Example 3 5 (Example 3) 5 - Fit to 1,000 ml Comparative Example 1 - - - Fit to 1,000 ml Comparative Example 2 - - 1,000 Fit to 1,000 ml Comparative Example 3 - 5 - Fit to 1,000 ml

The subject fasted 12 hours before the start of the experiment and supplied only water. Each experimenter was ingested 100 ml of Preparation Examples 1 to 3 and Comparative Examples 1 to 3 above, and after 30 minutes, 100 ml of 40% alcoholic beverage was ingested. Carboxy methyl cellulose (CMC) also has a thickening and stabilizing effect when used in beverages and is widely used when preparing beverages. As a group to which only the CMC solution was administered was set separately. After 4 hours of alcohol consumption, whether headache or gastrointestinal discomfort was investigated through a questionnaire and is shown in Table 6 below.

Survey on Hangovers headache Responders (People) Very good Good usually Inadequate Preparation Example 1 6 2 2 0 Preparation Example 2 6 2 2 0 Preparation Example 3 6 2 2 0 Comparative Example 1 0 0 One 9 Comparative Example 2 4 3 One 2 Comparative Example 3 2 3 One 4 Gastrointestinal discomfort Responders (People) Very good Good usually Inadequate Preparation Example 1 6 3 One 0 Preparation Example 2 6 2 2 0 Preparation Example 3 6 One 3 0 Comparative Example 1 0 0 2 8 Comparative Example 2 4 2 2 2 Comparative Example 3 2 2 2 4

As can be seen from the above results, the extract is good for the hangover, it can be seen that reduces the headache and gastrointestinal discomfort that is the main symptom of the hangover than the conventional C products.

The preparation example for the hangover composition of this invention is illustrated below.

< Formulation example  1> Preparation of Pharmaceutical Formulations

 1. Preparation of powder

2 g of Echinacea extract

10 g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Echinacea Extract 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional tablet production method.

3. Preparation of Capsule

Echinacea Extract 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

< Formulation example  2> Manufacture of food

Food products containing the extract of Echinosophoraea of the present invention were prepared as follows.

1. Preparation of Cooking Seasonings

10-15 parts by weight of the extract of Echinosophora japonica was prepared by participating in 100 parts by weight of cooking seasoning for hangover.

2. Manufacturing of Flour Foods

0.5 to 5 parts by weight of Echinacea extract of the present invention was added to 100 parts by weight of wheat flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

3. Preparation of soups and gravy

0.1 to 5 parts by weight of extract of Echinosophora cava of the present invention was added to 100 parts by weight of soup and gravy to prepare a processed meat product for health promotion, soup and gravy of noodles.

4. Manufacture of dairy products

5 to 10 parts by weight of Echinacea extract of the present invention was added to 100 parts by weight of milk, and various dairy products such as butter and ice cream were prepared using the milk.

5. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then powdered into powder having a particle size of 60 mesh.

The extract of Echinacea ginseng extract was concentrated under reduced pressure and concentrated in a vacuum concentrator, dried by spraying and a hot air dryer, and ground to a particle size of 60 mesh using a grinder to obtain a dry powder.

The grains, seeds, and Echinacea extracts prepared above were formulated in the following proportions.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Dry powder of Echinacea extract (3 parts by weight),

Ganoderma lucidum (0.5 parts by weight),

Sulfur (0.5 part by weight).

< Formulation example  3> Manufacture of beverage

1. Preparation of carbonated drinks

5 to 10 parts by weight of sugar, 0.05 to 0.3 parts by weight of citric acid, 0.005 to 0.02 parts by weight of caramel, 0.1 to 1 parts by weight of vitamin C, 1 to 5 parts by weight of the extract of Echinacea, and 79 to 94 parts by weight of purified water A syrup is made, the syrup is sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and 0.5 to 0.82 parts by weight of carbonic acid gas is injected to give a carbonated beverage containing the extract of Echinacea ginseng extract according to the present invention. Prepared.

2. Preparation of hangover for functional drinks

0.1 parts by weight of vitamins, 5.8 parts by weight fructose, 3.8 parts by weight of white sugar, 0.12 parts by weight of citric acid, 0.03 parts by weight of malic acid, 0.04 parts by weight of sodium citrate and 0.02 parts by weight of gardenia blue pigment, measured by combining 3 parts by weight of the extract of the present invention Completely dissolved in water. The dissolved beverage was adjusted with purified water so that the total amount was 100 parts by weight.

3. Manufacture of health drinks

10 parts by weight of subsidiary materials such as 0.5 parts by weight of liquid fructose, 2 parts by weight of oligosaccharide, 2 parts by weight of sugar, 0.5 parts by weight of salt, and 75 parts by weight of water, and 10 parts by weight of Echinacea extract are homogenously sterilized and then packaged into small bottles such as glass bottles and plastic bottles. Packaged in a container to prepare a healthy drink.

4. Preparation of Vegetable Juice

5 g of Echinacea extract of the present invention was added to 1 L of tomato or carrot juice to prepare a vegetable juice for health promotion.

5. Preparation of Fruit Juice

1 g of Echinacea extract of the present invention was added to 1 L of apple or grape juice to prepare a fruit juice for health promotion.

The preparation example for the antioxidant composition of this invention is illustrated below.

< Formulation example  4> Preparation of external preparation for skin

1. Preparation of Cream

Cetostearyl alcohol 2.8 parts by weight

Milnan 2.6 parts by weight

Stearic acid 1.4 parts by weight

2 parts by weight of glycerol phosphate monostearate

Fiji-100 Stearate 1 part by weight

Sesquioleate sorbitan 1.4 parts by weight

4 parts by weight of jojoba oil

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

2 parts by weight of macadamia oil

Tocopherol acetate 0.2 parts by weight

0.4 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methyl paraben 0.05 parts by weight

Glycerin 6 parts by weight

0.2 parts by weight of d-pandenol

4.6 parts by weight of Echinacea extract

0.2 parts by weight of triethanolamine

pt 41891 0.2 parts by weight

pH ₂ O 46.05 parts by weight

2. Preparation of Lotion

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Lipophilic monostearic acid 1.8 parts by weight

Fiji-100 Stearate 2.6 parts by weight

0.6 parts by weight of sesquioleate sorbate

4.8 parts by weight of squalene

2 parts by weight of macadamia oil

2 parts by weight of jojoba oil

Tocopherol Acetate 0.4 parts by weight

0.2 parts by weight of methylpolysiloxane

0.1 parts by weight of ethyl paraben

0.1 parts by weight of propylparaben

1,3-butylene glycol 4 parts by weight

0.1 parts by weight of methyl paraben

Xanthan Gum 0.1 parts by weight

Glycerin 4 parts by weight

0.15 parts by weight of d-pandenol

Allantoin 0.1 part by weight

3.5 parts by weight of Echinacea extract

4 parts by weight of carbone (2% aq.Sol)

0.15 parts by weight of triethanolamine

3 parts by weight of ethanol

pt 41891 0.1 parts by weight

pH ₂ 0 48.3 parts by weight

As described above, the extract of Echinacea extract, which is extracted from water, alcohol or a mixture thereof, increases the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase, thereby promoting alcohol metabolism in vivo, thereby reducing blood alcohol and acetaldehyde concentration. It can be used as hangover relieving agent and can also be used as an antioxidant activator because it shows excellent radical scavenging ability. Echinacea extract of the present invention can remove the hangover more efficiently than the commercial hangover drink, and can be usefully used as an antioxidant activator without side effects by effective radical scavenging.

Claims (11)

Hanging ginseng ( Echinosophora koreensis ) water, C ₁ to C ₃ Hangover relief composition comprising an extract of Echinosophora obtained by extraction with a lower alcohol or a mixed solvent thereof as an active ingredient. The composition of claim 1, wherein the lower alcohol is methanol or ethanol and refluxed for 2-4 hours. The composition of claim 1, wherein the mixing ratio of water and lower alcohol constituting the mixed solvent is 2: 8. The method of claim 1, wherein the hangover is resolved by increasing the activity of the alcohol dehydrogenase. The method of claim 1, wherein the hangover is resolved by increasing the activity of acetaldehyde dehydrogenase. delete delete delete delete Hangover ginseng extract of claim 1 as an active ingredient hangover remedy health food. delete

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