JP2002104924A - Photo aging-preventing agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a new and safe photo aging-preventing agent having an excellent photo aging-preventing effect, preventing wrinkles and slack caused by an irradiation with an ultraviolet light and improving skin roughness, dry skin, etc., maintaining flexibility and can be utilized for the prevention and treatment of diseases associated with the photo aging-preventing effects, and further a cosmetic composition or food. SOLUTION: This photo aging-preventing agent contains a magnolia bark extract or a mixed extract obtained by simultaneously using >=1 kind selected from plant extracts from Isodon japonica, Scutellariae radix, Panax ginseng, Hypericum erectum, comfrey, salvia, Perilla frutescens crispa, linden and moutan bark together with the magnolia bark extract, and the cosmetic composition and food containing them are also provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel and safe plant extract or a photoaging inhibitor, a cosmetic composition or a food or drink containing the same.

[0002]

2. Description of the Related Art "Aging" is definitely occurring in everyday life. “Aging” can be broadly classified into “physiological aging” and “photoaging”. “Physiological aging” is a biological decline caused by aging, and cannot be avoided by everyone. On the other hand, "photoaging" is aging due to the influence of ultraviolet rays, and greatly differs depending on the living environment and customs so far, which influence the amount of ultraviolet irradiation.

[0003] It is generally said that skin deterioration begins around the age of 23, although there are individual differences. There is no doubt that long-term ultraviolet irradiation accelerates skin aging. The most aged parts of the body are the back of the hand and the back of the neck. Ultraviolet light illuminates the exposed skin evenly. Unnecessary UV radiation when you are young has certainly had an adverse effect after years.

[0004] At present, technologies for suppressing aging by scientifically elucidating "physiological aging" and "photoaging" are being actively developed by cosmetic companies and others. In particular, cosmetics incorporate techniques for overcoming aging and maintaining beautiful skin. Prevention of photoaging by measures against ultraviolet rays includes selecting cosmetics that have a sunscreen effect and using hats and parasols on a daily basis. It is a common wisdom that ultraviolet light is "harmless and unreasonable."

[0005]

As described above, skin that has been photoaged by ultraviolet light loses its flexibility and can be wrinkled or sagged. At this time, thickening of the epidermis is observed in the skin. For this reason, development of a substance that suppresses epidermal hyperplasia and maintains skin flexibility even when exposed to ultraviolet rays, that is, has a photoaging protection action, has been awaited.

[0006]

[Means for Solving the Problems] In view of such circumstances,
The present inventors have attempted to screen a natural plant for a substance that is excellent in photoaging protection and has no side effects, and has been studying a novel plant extract and its components. As a result, it is induced by UV irradiation by using one or more plant extracts selected from the extract of Kokumoku, the extract of Komoku, and the extract of Ginkgo biloba, Ogon, Panax ginseng, St. John's wort, Comfrey, Salvia, Perilla, Perilla, Bodaiju and Bompi. Inhibition of epidermal hyperplasia was observed, and it was confirmed to have a photoaging protection effect. The present invention has been completed by providing a highly safe photoaging inhibitor, a cosmetic composition, and a food or drink, which can prevent wrinkles and sagging due to ultraviolet irradiation and maintain flexibility.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The plants used in the present invention are as follows, and all of them are flowers, spikes, peels, fruits, stems, leaves, branches, branches and leaves, stems, bark, rhizomes, root bark, roots and seeds. Alternatively, whole plants can be used, but those available as crude drugs may be used.

"Kouboku" is a magnolia family (Magnolia)
ceae) Magnolia plants of the genus Magnolia (Magnolia obovata Thunb.), Sengoku, Kohokoku Koboku, Karaho (Magnolia officinalis Rehd. e)
t Wils.), Unboku, Unshukoboku (Magnolia)
officinalis Rehd. et Wils.var.biloba Rehd. etWil
s.), etc., using all the flowers, spikes, pericarp, fruits, stems, leaves, branches, foliage, stems, bark, rhizomes, root bark, roots, seeds or whole plants of plants generally called "Kouboku" be able to. In addition, congeners can be used.

[0009] "Emiso-cho: prolonged life grass" refers to the Labiatae family (L
abiatae), a plant of the genus Rabdosia (Rabdosia japonicus (Burm.f.) Hara (= Isodon japoni
cus Hara) ”or Isodon trichocar
pus Kubo) or whole plants.

"Ogon: Yellow Gon" means Labiatae (Labia
tae), a plant of the genus Scutellaria (Scutellaria)
(Scutellaria baicalensis Geoegi) without roots.

"Ginseng: Panax ginseng" means a plant ginseng "Panax ginseng CAMey. (= Panax) belonging to the genus Araliaceae and Panax L.
schinseng Nees) "" Alias: Panax ginseng ".

"Hypericum perforatum" refers to Hypericum family (G
uttiferae), Hypericum perforatum (Hypericum) plant Hypericum perforatum (Hypericum erectum Thunb.), Hypericum perforatum (Hypericum perforatum L.), Hypericum perforatum L. var angustifoliom DC
Use whole grass.

The term "comfrey" refers to the family Boragin
aceae), plant comfrey of the genus Symphytum
(Symphytum officinale Linne) leaves, branches and stems are used.

"Salvia" is a plant of the genus Labiatae and Salvia officinalis.
L.) "Alias: Sage" flowers, leaves and whole plants are used.

"Perilla: Shiso" refers to Labiatae,
Perilla plant (Perilla frutescens Britt)
on var. acuta Kudo) or other related plants.

"Bodaiju: Bodaiju" is a linden family
(Tiliaceae), a plant of the linden genus (Tilia)
(Tilia platyphyllos Scop.)
ordata Mill.), Tilia europaea
L.) or other closely related flowers, fruits and bark.

[Pinpi: Peony] is a family of Pae (Pae)
oniaceae), a plant belonging to the genus Paeonia, and the button `` Paeoni
a moutan Sims (= Paeonia suffruticosa Andrews) "root bark is used.

The various plant extracts of Kokuboku, Echinacea purpurea, Japanese gourd, Panax ginseng, Hypericum perforatum, Comfrey, Salvia, Perilla, Bodaiju and Pontopia used in the present invention are those obtained by pruning various parts of a plant as it is or after crushing. Alternatively, it is extracted with a solvent as it is or after pulverization.

Examples of the extraction solvent include water, alcohols (eg, lower alcohols such as methanol, anhydrous ethanol and ethanol, or polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin), and ketones such as acetone. , Ethyl ether, dioxane, acetonitrile, esters such as ethyl acetate, organic solvents such as xylene, benzene, chloroform, etc. can be used alone or in any combination of two or more. Can also be used in a state where.

The production method is not particularly limited, but may be usually within the range of the boiling point of the solvent at normal temperature and normal pressure. After extraction, filtration or use of an ion-exchange resin is carried out.
A solution, paste, gel, or powder may be obtained by decoloring / purifying. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

[0021]
The plant extracts of Panax ginseng, Hypericum, Comfrey, Salvia, Perilla, Bodaiju, Bompi, can be dried, concentrated, or diluted according to the type and form of the applied photoaging inhibitor, cosmetic composition, or food or beverage. It should be done and adjusted.

[0022] The plant extracts of the present invention, Kokuboku, Japanese radish, Japanese gourd, Panax ginseng, Hypericum perforatum, Comfrey, Salvia, Perilla, Bodaiju and Botanpi,
In addition to being able to be used as a photoaging inhibitor as it is, it can be contained in cosmetic compositions and foods and drinks, but its content is not particularly limited. It varies slightly depending on the type, quality and expected degree of action, and is usually 0.001% by weight or more (hereinafter, expressed as%), preferably 0.01 to 50%. The content is 0.00
If it is less than 1%, the effect cannot be expected sufficiently.

The photoaging protection agent, the cosmetic composition and the food or drink of the present invention may further contain, if necessary, the components and additives described below as long as the effects of the present invention are not impaired. Agents can be arbitrarily selected and used in combination.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil, cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow fatty acid, kukui nut oil, safflower oil, shea butter, liquid shea butter, soybean oil, camellia oil, corn oil, rapeseed Oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, or hydrogenated products of these oils (hardened oil, etc.) )etc.

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, rice wax, squalene, squalane, pristane, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokelide, ceresin, microcrystalline wax and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid And synthetic fatty acids such as 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) alcohols ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharide or water-soluble polymer compound Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose or an ester thereof, trehalose or a derivative thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) to which an alkylene (C2-C4) oxide such as ethylene oxide is added. )
Chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or its salt, hyaluronic acid or its salt, chondroitin sulfate or its salt, heparin, ethyl cellulose, methyl cellulose, carboxymethyl cellulose , Carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyalkylene oxide such as polyethylene oxide and polypropylene oxide Or its crosslinked polymer, carboxyvinyl polymer, polyethylene N'imin like.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type nonionic Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactants, surfactants containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin A
1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1), riboflavin (vitamin B2), pyridoxine (vitamin B)
6), cyanocobalamin (vitamin B12), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: vitamin C acid or its derivative, vitamin D group: ergocalciferol (vitamin D2), cholecalciferol (vitamin D3),
Dihydrotaxelol, vitamin E group: vitamin E or its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K
2), menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid, vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, etc., and their sulfates, phosphates, nitrates, citrates,
Or amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are processed in a conventional manner according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, powdering, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, the processing to be performed later, and the like. Usually, water and a water-soluble organic solvent (eg, ethanol) are used. ,Propylene glycol,
It is desirable to use one or a mixture of two or more selected from 1,3-butylene glycol). However, when the content of the organic solvent is not preferable depending on the application,
Only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in any mixed solution with water, or may be extracted and extracted.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress by fragrance, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, relief of fatigue, promotion of blood flow,
In addition to cosmetic effects such as warm bath effect, fragrance, deodorant, thickening,
Antiseptic and buffering effects can also be expected.

In foods and drinks, nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and other healthy and cosmetic effects, improvement in taste, provision of color tone, aroma and gloss, stabilization, It can be used for purposes such as thickening and preservation. Furthermore, in addition to this, we expect various cosmetic and pharmacological effects of each raw material known so far, and aim to increase the intended effect by combining them,
Alternatively, it is possible to provide a product that is expected to have a multifunctional effect.

As specific plants (crude) as raw materials,
For example, almonds (Hentomo), eye (Aiba), Blue Kazura
(Seifu Fuji), Aoki (Aoki), Aokiri or Kenasia Ogiri
(Otogi), Aotsujirafuji (Kibo), Acacia, Akashoma (Akamasa), Akasuri `` fruit '', Red clover, Red grape, Akaminoki (Logwood), Red moss (Red bud), Red moss (Akane grass root), Red moss Ground yellow), Agi (Awei), Aki Larch, Akinire (Latinaceae),
Aguay Guacu, Aguahe (Omitengu palm),
Akebi (Kidori), Asa (Jin Asako), Malva Asagao or Asagao (Toshibushi), Hydrangea (hydrangea), Ashitaba (Asahi),
Azuki (red azuki), acerola, asenyaku (Asenyaku), acira (shokuyoukanna), anise, abiu, abiurana, rape, avemaki `` fruit '', avocado, ama, amacha (sweet tea), amachaworu, amadocoro (tamatake), Amana (Kikomiko), Amaranthus (Amaranthus), Amaranthus hippochondriacus, Haribiyu, Amaranthus hippochondriacus, Amigasa lily or Bimo (shellfish),
Aristolomelia (Yurizisen), Arcana (Alcanna), Algarrobo (Cabbage), Altea, Arnica, Alpinia or Katsumadai (Souduq), Aloe (Length), Aloe vera, Angelica, Apricot or Hong apricot (Anjin), Ansokkou (Azusa), Yellow sapote , Ikakozorina (ground bile head), Epimedium or Epimedium
(Inyokaku), Igusa (Wicked grass), Knotweed (Tiger root),
Strawberry (first place), strawberry, fig (Fig.
Leaf)), Ichihatsu (first time), Ichibi (Fruit Aoi), Ichiyakusou (one medicinal herb), Ginkgo (Ginkgo `` seed, leaf ''), Itohimemegi (Enshi), Carob, Inu-zuna (Telelexi), Inubeyu (Sorainubiyu), Rice `` seed, seed coat '', Inondo `` seed '', Ibuki-kusou-so, nettle, ylang-ylang, iwa-tobacco (iwatsuchi-chi), iwahiba or iwamatsu (makikashiwa), oolong tea, wikyo (fenka), wheat (tibusanoki), ukiyagara (miyoko) ), Uguisukagura "fruit",
Himeuikyo, Turmeric (Utsukin), Duckweed (Floating), Usubasaishin or Keirinsaishin or Shousaisaishin (Spicy), Usvenia oi, Usvenitai oi, Utsubogusa (summer dry grass), Udo or Shishiudo (Qang, Independent, Tang Independent) ), Unyadegato (Unkaria, Cat's claw), Uba tea, Ume (Ume `` seed, pulp ''), Vladimir, Owaurushi (Kusaco peach), Unshu mandarin (Chen skin), Estragon, Ezocogi (Ezo Goka), Echinashi (Hosobamurasakisakirengiku) , Enishida, Enokitake (Enotake), Ebisugusa or Cassia Tora (Kyoko), Elcampre, Elderberry `` fruit '', Elemi, Elingy or Prelotus elinge, Endu (Solanaceae, Sophoraceae rice), Ogi or Kibanaugi (yellow) Hoki), Ousei (Naruko Yuri, Kagi Kurumabanaruko Yuri), OuHisasa Yuki or Sasanoyuki, Coptis (Coptis), Oogatahouken, giant Karasuuri (Karokon), Ooguruma (civil engineering incense), giant Tsudzurafuji
(Self-defense), psyllium (vegetative child, vegetative grass), toucan hazel (haruko), toucan cactus, obanana okera or okera
(White jujube), giant sedge beetle (banaba), giant beetle "fruit", giant squirrel (cereal sperm), giant caterpillar, hawthorn or hawthorn (mountain sprouts), barley
(Barley), oka, okazeri (snake bed), okra `` fruit '', oguruma (turnover), carrot (carrot), odoris
(Intermittent), Onami fir (blue ears), Onigorumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Oninoyagara (Tenma), Onyuri or Sasayuri or Hakatayuri (Yuri), Ononis, Halibut (Hihayu), Ominaeshi ), Oyuco, Dutch mustard (Watercress), Dutch jelly, Dutch honeywort, olive `` fruit, seed, leaf '', oregano, orange `` fruit, peel, '' Kaikai-jiou (ripe yellow), cacao `` fruit, peel, seed '', Kaki (persimmon `` leaf ''), Kagikazura (fishing hook), kakidooshi or kantosou (rensensou), gaku hydrangea, cassia, casino aki (enamel berry) (Leaf), cascarilla, cascara sagrada, gypsophila, gypsophila, kanikusa (kinsato), kaniwa, carnation, kanokoso (valerian grass root), birch or weeping birch (white birch), moth Vera, pumpkin, capoknoki `` seed '', kahoku sansho (shopper), cattail (bamboo yellow), chamomile or roman chamomile, kamiyatsude (weed), camucum (duckling), color, karakakeman, crow squirrel or cinnamon squirrel (flowering melon), Karasubishaku (midsummer), oats, guarana `` seed '', karahoo (thick), karaya, galyu, karin (cucurbit), garcinia, kawamidori, kawara psycho (geiryo greens, vermilion grass), kawajisha, kawataketake, kawaranashiko (stone bamboo) ) Or Ezokawaranadeshiko (Quaku, Quumiko), Caroline carrot (Qingko),
Japanese mugwort
(Licorice), cantaraasa, candelilla, cantola, canna, raspberry (ezo strawberry, strawberry strawberry, shrimp strawberry, pear strawberry, maple strawberry, european strawberry), kiwi `` fruit, leaf '', kiwi sap (urorone), kikyo (bellflower, bellflower) ), Chrysanthemum (chrysanthemum, sycamore, narcissus), chrysanthemum, catalpa (Azumitsu), sagebrush (yotei), pheasant (kinkumi), kizuta, kidachi aloe, edachi mint, kina, chinoki (shincona, red linden), quinoa (quinoa) , Yellowfin (yellow),
Gymnema sylvesta, kimmenkaku, cabbage, cabbage `` immature fruit '', cucumber, gyoryu (Nishikawayanagi, teriyaki), Kiraja saponaria, Kiraya, Kiranso (gold acne grass), kumquat `` fruit '', ginsekai, kimbusen,
Betel, golden hibiscus (following herbs), guapeba vermelha, guava `` fruits '', guayule, quelux infectria (gallicate), cucuinuts, wolfberry
Gugiko, gugi leaf, ground bone bark)
Kudzu (Kakkon), camphor tree, gooseberry `` fruit '', ginseng (yellow flower), cutie river, gardenia (garden), kunugi (bokusou), cupuas, kumazasa, kumamatsura (horse whip grass), clara (difficult) , Cranberry `` fruit '', chestnut `` seed, fruit, astringent skin '', curculigo latifolia `` fruit '', grapefruit `` fruit / leaf '', buckthorn, black crocodile (rescue), cloves (cloves, cloves),
Gumbinazuna (Sekimei, Sekimeishi), Kaigai (Thorns, Thornspring), Celosia (Corolla, Chicken corolla), Geckavidin, Gecko (laurel), Kenashisarutibara (Tokubukuryo, Yamakiko), Gentian, Gennoshoko (Old grass), Kemponashi (Kigushi), Koukisekikoku, Kishumitsukan (Koji, Tachibana, Obenimikan, Fukuromikan, Sagamikouji, Ponkan, Santara (Tachibana bark)), Koushinbara (Monthly flower), Kosuihakka, Kouzo `` fruit '', Kocha (tea), Kohone (river bone), kouhon (straw and karawara), kouriang, kourikyo (takara ginger), cilantro (fruit), coconut (huohren), lingonberry (koshibachi), coconut `` fruit '', goshyu (goshuyu), Gosho strawberries (covered bonko), pepper (pepper), cosmos, copaiba balsam, coffee "seed, leaves", kobushi or moss Ren (fist), burdock (burdock, Goboko), Kobotandzuru, sesame seeds (sesame), figwort (Scrophularia buergeriana Miq), Gomishi (Schisandra), kadsura japonica or Binankazura or Matsubusa, wheat (wheat),
Rice or rice bran `` red bran, white bran '', rice oil, cola acuminata `` seed '', cola vera `` seed '', colony llah, fenugreek `` fruit '', colombo, conzulango, kelp, konjac, sisal (sisalasa), saxima button or Synapse or cinnamon tree (weird spirits), cherry (Ooyamazakura, Yamazakura, Oyamazakura,
Ed Higashi, Mamezakura, Miyamazakura, Someiyoshino, Takanezakura, Kasumizakura, Kohigan, Satozakura, Kanzakura `` Leaves, flowers, fruits, bark (cherry bark) ''), cherries, pomegranate, sasa, sasanqua, sagemodaka, sweet potato, sugarcane, Sugar beet, jujube jujube (acid jujube), saffron (safflower, west safflower), sapodilla, sapotea marillo, pomelo "fruit", saponso,
Salmon berry `` fruit '', sarasin ginger (masa), sawagyoukyo (yamachona), sawagurumi (yamawalnut), sankaku cactus, sangre de grad (croton), sansouyouso, sanshin ginseng (37 ginseng), sanshuyu ( (Shushuyu), Sansho (Sansho), Sandscon (Yamazone), Shea (Karite), Cyano (fruit), Shiitake (Shiitake), Shion (Shion), Dikitaris, Shikunshi (Tsukimiko), Aodiso or Chirimenzo or Catamendiso ( Perilla leaves, perilla), rosewood, linden, Chinese pine, Chinese forsythia,
Shimeji (Hinshimeji, Shakashimeji, Hatakeshimeji, Oshiroishimeji, Bunashimeji, Homujimesi, Shirotamogitake), Shimokren (Spicy), Spiraea, Potato,
Peonies (shakuyaku), shajin (shasan), jasmine (matsurika), janohige (wheat gate winter), gypsophila,
Sukushamitsu (sand flakes, shrunk sand), juzdama, palm `` fruit '', ginger (ginger), ginger hydrangea (Tsuneyama), shobu (iris, iris root), shojiku `` fruit '', shirakashi `` seed '', shirogoshi `` seed '' , White clover (clover), white adzuki bean (chicken bone), white iris (small iris), white banana (flower), white banana (flower), white nananten (minamitenmi), cinchona sacsylbra, ginkgo biloba (mizuka, mikaka, shencho), cinnamon, cinnamomu・ Cassia (cinnamon bark), watermelon (watermelon), honeysuckle (gold and silver flowers, Shinobi winter),
Sorrel (acid model), swim berry `` fruit '', star apple, stevia, strawberry `` fruit '', suzu psycho (Xu Chang Lord), horsetail (infestation), purslane (horse dent, horse dent), plum `` fruit '', pear Japanese red pine “cone”, larch, vegetation, walnut, walnut, hawthorn, dandelion, horse chestnut
(Marronnier), pear `` fruit '', snow prunus sylvestris, Atlantic elderberry (elder), Atlantic spp.
Horseradish, Sekisho (Iris), Sekkoku (Ishimasu), Cedron (Bowshukuboku), Zeniaoi, Hirohase Nega, Senega, Seri, Celery, Sengoku (Kawakyu), Sengshinren (Shinshinren), Sengdan, Senninsaboten, Senna , Leaves '', Senninsou (great tate), Japanese cabbage (medicinal herb), squid (grass), elephant, buckwheat, buckwheat `` seed seeds '', somenimo, rhubarb (daihuang),
Radish, soybean, daidai (orange, kikomi), takasaburo (dry lotus), takatodai (daigeki), takawarabi (gull spine), dark sweet cherry `` fruit '', octopus mallow, jacuzzi thyme (thyme, momoka), Hikai), Twill willow, tamarind `` seed '', onion, tamashiba (spicy), taranoki `` fruits, leaves, root bark '', dahlia (tenjikubotan), tarui, tanshiuchiwa, tanjin (dansin), dandelion (gakko British) or white dandelion or Moko dandelion, dammar, cherry "fruit", Tigaya "fruit, root,
Buds '', carrots (bamboo ginseng), chicory, chancapiedra (golden ginseng), ginseng
(Ohta), Korean lance (Wuyi), Artemisia (Aiba), Choreimaitake (Irei), Japanese cricket, Tsukimisou, Tsukuritake (mushroom), Camellia, Camellia,
Tsurukusa (lacquer primrose), twigweed (duckling grass), vine adzuki (red azuki bean), tsuldokudami (what crow), tsuruna (banyou), tsujin carrot (four leaf ginseng), tsuwabuki, deiko, teuchigurumi, duberry `` fruit '', tulip, chocolate Nostay, Tengusa, Tencha (Tencha), Tendai Yaku (Wakayaku), Pepper (Winter Gourd), Toukagi Kazura, Pepper (Pan pepper), Touki (Toki), Tokinsenka (Marigold), Tounabena (Kawabashi), Corn or corn hair (nanban hair),
Ligustrum (Female Sadako), Eucalyptus or Korean Rosemary (Ryubin), Tokusa (Pirates), Dokudami (Jyuku), Tokon (root), Toshishi or Mamedaoshi or Porpoise, Eucommia (Tonaka `` bark, leaves, roots '') , Ash (qin bark), tomato, tragacanth, triacanthus `` seed '', eustoma, tormentilla, dronoki, troloioi, tongue bean, Niseria berry `` fruit '', potato or yamanoimo (herbal medicine), potato bloom (butcher bloom), naginata koju, Japanese rape, oilseed rape, oilseed rape or mandarin
(Swords), Natsumikan, Jujube (daijutsu), Naniwaibara (Kinzakurako), Navena (Continuous), Nameko, Narukoyuri (Yoshisei), Peanut (peanut), Nanten (Minami Tenmi), Nanbankarashi (ramie), odor Violets, bitter oysters (bitter wood), sagebrush
(Bitter mushroom), nutmeg, kei or nikkei or Ceylon nikkei or yab nikkei (cinnamon bark) or keiseki (keishi), leek
(Niriko), elderberry (bone-bearing tree `` fruit, flower, stem, leaf ''), garlic (garlic), nurde (five times), leek, nemoki or neb or nebi or nemuriki or digokbana (health), thistle (Oji) , Noibara (real fruit), Nozenkazura (Ryoshohana),
Lacewing, Yarrow, Sawdust (Maehu), Nobara, Nomomo, Pineapple `` Fruit '', Hibiscus (Bussouge, Furinbussouge, Roselle), Hirindou,
Hauchiwa, Pau Doce, Hakamauraboshi (bone crushing supplement), Hakusen (ringworm skin), Huckleberry `` fruit '', Hakobe (proliferation), hazel (Haruko), Hashiridekoro (roto root), basil, lotus (lotus, lotus, (Renko), parsley (Netherlands jelly), naked mussels, batata, bees or medakes (bamboo-boiled), patchouli, mint (lightly loaded, lightly loaded leaves), barley (yokuinin), hanasuge (chimochimo), banana, hana mint, hanabishi (swordfish, Shititsuri), vanilla bins, papaya, japonicus
(Mouse malt grass), pahalobobo, hub `` whole plant, stem, leaf '', paprika, hamago or honeysuckle
(Kazuke), Hamabishi (Situriko), Hamanas (Maikai flower), Hamabo fu (Hama wind), Hamamelis, Palm, Rose (Roses), Ballata, Balata gum tree, Haratake (Haratake, Shirooharatake, Usukimori nokasa), Haran, Paliurus ( (Prunus avium), Parietalia,
Balsamina (Tsurureishi, Nigari), Harunile (Yellow, Yuhakuh, Yuba), Barbasco, Harindou, Handaikai (Greaper Sea), Breadfruit, Hiougi (shadow), Lycopodium ginseng (partisan), Pecan nut, Lycopodium (stone) Hirugaru, Tang Jiasha, Higurumadaria, Hishi (Hishimi), Pistachio, Beet, Hitotsuba (Ishire), Hitotsubashienida, Daisies (Daisy), Hinata Kozuchi (cow knee), Hinoki,
Hiba, Castor, Sunflower, Bell Pepper, Himeuzu
Himegama (Kaba), Himematsutake (Kawariharatake, Hiromatsutake), Pimenta "Fruit", Juniper, Biacucu, Hiyu "Fruit", Below Oy, Hiroha Okinagusa
(Shiratoo), loquat `` fruits, leaves, stems '', betel nut (large clothing skin,
Betel nut), Pu'er tea, Sperm kazura (southern rattan), Butterbur,
Butterflies (winter flowers, winter leaves), Fujibaka (Ranchus), Fuji beans (tonboshi), Fujimodoki (Chowsia zakura, cicada flowers), grapes `` fruit, pericarp, seeds, leaves '', beech, Fuyumushinatsukutaketake (Nasamushi), Brazil licorice , Brazilian carrots, black currant `` fruit '', blackberries, blanin, plum `` fruits '', flucellaria, blueberries (Prunus aegypti), prunes, floral blankas, blonde psyllium, bundou (green beans), hazelnuts, loofah, safflower (safflower) ), Henneken, Belladonna, Berry `` fruit '', Persair, Perbianberg, Pereschia grandifolia, Benquisou or duckweed (Keiten), Boysenberry `` fruit '', Broomweed or Elderweed or Nendow or Hawakigi kokia (skin), Balsam (Hosen, acute child, Honekusa), Pouteria-Sapota, Pouteria-Rukuma, storm (wind), spinach, ground cherry (Toro root), Magnolia obovata (KazuAtsushiBoku, Park), blur
(Gourd), Hosoba Aburagiku (bitter gourd), Hosoba Oguruma,
Hosovanaokera (Aojutsu), Hosobanokirinsou (Kageten Sanshichi), Hosobabarengiku, Button (peony "flowers, leaves, stems, bark"), hops, jojoba, borage (ruriichisha), Bordeaux, whiteberry berries "fruit", Hongoniku (Meat Ji Ryo, Daigei), Hon Sekkoku (Steel Stone Stone, Sparrow Mountain Stone Masu), Mysuruten Nanshaw (Tennan Star), Maitake (Maitake), Maoh
(Mao), maca, macadamia nuts, margaret (mokshungiku), macri (sea grass), maguwa (mulberry white bark `` bark,
Leaf)), Magnolia splengheri, Masalampuba,
Masalan Zuba de Ceara, Masheira de Boi, Mashua (Tamanousenhallen, Kyukonkinrenka), Matatabi (Kiten Tate), Matsukasa, Pine `` leaves, bark,
Roots, Matta Oryo, Matsuhodo (Bukuryo), Matico
(Coldon Sillo), Mayorum (Hanamint), Malvanozadin (Difficult), Mulberry `` Fruit '', Quince, Malein (Beloyd Mozuica), Mango, Mangosteen,
Mansagna (Andesukamitsure), Mansugurumi,
Mandarin `` Fruit '', Mannentake (Lingzhi), Pheasant (Kikomi `` Fruit ''), Mishima Psycho (Saiko), Mizuobako or Mizuasagao (Ryungoshi), Mizokakushi (Hanbe Lotus), Misohagi
(Chikuna), beet willow or elder willow (edition), honeywort, honeywort, midori sapote, green peppermint, mimosa, mioga, miracle fruit `` fruit '', myrrh, mirobaran, oak, oak, oak
(Extended skin), munya, purple (purple root), purple or purple purple pearl, purple maize,
Murasaki Natsufuji (Kunming chicken blood wisteria), Megakanza opuntica, Mehajiki (Emperor grass), Mebogi, Melaleuca, Melissa, Melillot, Melon `` fruit '', Mokoyomigi, Mosobuchikku, Mokkillin, Moje (Kyoboku), Mokko (Mokuko), Maple Butterfly, Peach (peach `` leaves, seeds,
`` Flowers, fruits ''), sprouts, morello cherries `` fruits '', moroheiya (burlap), yakawamurasakiimo, yakuchi (masuti), cornflower (cornflower), cornflower mint, yacon, yashabushi (arrow wheel) or himeyashabushi or giant yashabashi Fruits, peels, and ears "
(Octagonal gold plate), mistletoe (parasitic willow), willow (river willow,
Twill willow, weeping willow, red eye willow, cat willow, dog korean willow, kinuyanagi, koreyanagi, unryuyanagi, miyayamayanagi, palm, yamayanagi, giant willow, tyrikkinyanagi, foxeye willow, foxgall
(Purple gold beef), yabu tobacco (crane squirrel, Tenna sei), pokeweed (land), yamahanoki (yamaharu), yamamomo (yangmei skin), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu Fruits, Lily, Yorugusa, Artemisia (Aiba), Lygan (Rainuma), Lime `` Fruit '', Rye, Lacanca `` Fruit '', Raspberry `` Leaf, Fruit '', Latania (Clameria), Rakkyo or Shallot (Scallop), Lavender , Longan (longan meat), agave (Aono agave, fukurin agave),
Ryokucha (green tea), apple `` fruits, seeds, leaves, roots '', gentian, rubus or suavisimus (sweeten), litchi (lichi, lichi nucleus), lettuce (chisha), red currant `` fruit '', red pitaya, lemon `` Fruit ", lemongrass,
Forsythia or Chinese forsythia (forsythia), astragalus, waxby (wax plum), coconut palm, lokon (reed, azuki), loganberry `` fruit '', rosemary (mannan wax), rosehip (nobara), wasabi, cotton wisteria flower),
Waremokou (chiyu) and the like.

In addition, other seawaters, for example, seawater salt, dried seawater, inorganic salts obtained from the Dead Sea or the Atlantic or Pacific Ocean (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or mud (fango) , For example, sea mud or mud of various places such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango, etc. (Ingredients: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, potassium oxide, magnesium oxide , Calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic,
Wed), Shotoku stone etc.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaaonori, Susiaonori, Hiraaonori, Bowaonori, Hosoedaoonori), Anaaousa (Aousa) and Algae [Aosa] Kinds: Kombu (Macambu, Lysicum, Hosomekombu, Mitsuikonbu), Wakame, Hirome, Aookame, Giant Kelp (Macrocystis pilifera, Macrocystis integrifolia, Neocitys luetokeana), Hijiki, Hibamata, Umiuchiwa, Usubaumiuchiwa Kilevanoumi prickly pear, Akaba prickly pear, Konau prickly pear, Okina prickly pear, Usuki prickly pear, Echinoki prickly pear], seaweed [Red algae: Hijirimen, maxa (Tengusa), Hiracusa, Oniku , Obakusa, Kataobakusa,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Juniperus giraffe, Tsunomata, Obatsunomata, Tochaka (Yahatsutsunomata), Ezotsunomata, Togetsunomata, Hirakotonota, Kotoji, Kotojita
Jareus Banori, Kagius Banori, Siusius Banori, Hyus Banori, Akamomininori] and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Tsurugimidro, Hibimidoro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: magnolia, asamidorigusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: agatamagu, iwata: husaiwata, suricogita, heraiwata, crocodile, crocus Sakibuchimil, Nagamir, Hiramil, and Casanori:
Casanori, Jusmo: Fusuzumo, Tamazumi,
Species of the genus Mizodzumo, genus Genus, genus Colecaetus, genus Ceratophylla, genus Ceratopogon: Rubiaceae, Rana genus: Rana genus, Hirano-Hana, Rana genus, Rhizophora, Genus genus, etc.

Cyanobacteria (Genus L .: L., L., L., L .: Kawatake, Ishikurage,
(Hassay, genus genus, genus racenmo (spirulina): spirulina, genus trichodesmium (aiakacio), etc.).

Brown algae (Piraella spp .: Piraella spp., Spirogyra spp.
Isogawara genus: Isogawara, Kuroshira genus: Gunsenkurogashira, Kashirazaki genus: Kashirazaki, Muchimo genus: Muchimo, Hiramutimo, Kebelligsa, Amidixa genus: Amidigusa, Sakibiroamiji, Sanadagusa: Sanadagusa, Fukurinamiji, Common genus: Artemisia spp.
Genus genus: genus pine, genus genus: genus genus: genus genus, genus okinawa, genus okinawa mozuku, genus nov. Ishige, Irolo, Icmegasa: Ichimegasa, Keyari: Keyari, Umibos: Umibos, Urushigusa: Ursigusa,
Species of Coleoptera, Tobacco moss, Laminaria spp. Genus Kagomenori: Kagomenori, Muraridori: Murachidori, Shakusugusa: Sharks
Genus Genus: Genus Genus, Genus Genus: Genus Genus: Genus Genus, Genus Kayamonori, Genus Kayamono, Genus Genus: Genus Ligustrum, Genus Tsurumo: Genus Tsurumo, Genus Anana: Genus Anastasia, Genus Suzume: Genus Missiscoma: Genus Missiscoma, Atsubumikonga, Kossula , Oni-kombu, Gohei-kombu, Naga-kombu, Pea-kombu, Ochimitsu-kombu, Toro-kombu genus: Toro-kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikuishicombe genus: Kikuishicomb, Nejirekombu: Nekorecome, Kuroshimeo : Nekoshikonbu,
Alame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakameezoishige: Ezoishige, Yabanemoku: Yabanemoku, Rapamoku: Rapamoku, Jiromoku: Uganomok, Jiromoku, Hiemomoku, Hokumoku, Tamamomoku Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametarawara, Tatsukuri, Yasumatamoku, Umitrano, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Saw-mokum, Obanoki-mimoku, Genus genus:
Bulumimo, genus cynomolgus: cynomolgus etc.).

Red algae (Genus genus: genus Ushikenori, Funori no genus, Amanori: Genus Asakusanori, Susabinori,
Genus Upirinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton: Milnoveni, Akebonomozuku: Akebonomozuku, Konahada: Haikonahada, Yogorekonahada, Aokonahada, Sea Species: Acacia sylvestris, Ochryllidae, Larvae genus : Larch, Fusanori: Fusanori, Nisefusanori: Nisefusanori,
Sodegarami: Sodegarami, rattles: Rattle, Hiragaragara, Hirohatamataki: Hirohatamaitadaki, Tamaitadaki: Tamaitadaki, Kakenokenori: Kaginori, Kakenenori, Tengusa: Himetengusa, Hitenugusa, Hitenugusaobana, Hitennakuobusa, Hitenuganugusa, Hitenugusaugou Genus: hibiroudo, himebiroudo,
Genus Isomomedogi: Genus Isomomedo, Genus genus: Genus genus, Ryumonsou: Ryumonsou, Hera Ryumon, Nisekarekigusa: Nisekaregusa, Okutsubara: Oba Otsubara, Akaba: Akaba, Malvaacaba, Naminohana genus
Naminohana, coral genus: rattles, genus cynosporium: shiogosagoromo, genus iwanokawa: esukiiwanokagawa, genus kainokawa: genus kainokawa: kaninote, genus coral: coral genus, coral mosquito, mosquito mosquito , Tambanori, slippery,
Fudarak, Malbahudarak, Isonohana: Nuracusa,
Black skull, scorpion scorpion, squash genus: squamous ibis, scorpion genus: scorpion genus: scorpion sculpture: chabo kintoki, squash, matsunori, komenori, tosakamatsu, tsutsumatsu, fushikintoki, tsunomukade, swordfish, swordfish, saccharomyx, skullfish Nagaobabane: Nagaobane,
Genus Funori: Hanafunori, Fukuronori, Makunori, Karenikusa: Kalexigusa, Tosokamodoki: Hosobanotosakamaki, Hirohanokasudo, Yatsudegatatosakamoki, Kurosakamoka, Nezashinotosakanodoka, Yukasinosa Modoki,
Genus genus: genus genus: genus: kinuhada, genus ezotosaka: genus ezotosaka, genus tsukasanori: genus california, giant porpoise, genus hagano genus: genus honoo, genus genus: genus lycopodium, genus genus Susqueke Beni, Okamuragusa:
Yamadagusa, Mirin genus: Mirin, Hosobamilin, Tosakanori genus: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka genus: Isomocca, Yukari genus: Yukari, Hosoyukari, Ibaranori genus: Ibaranori, Saidaibara, Tachiibara, Kagiinobalon, Kijunio genus Isanthus, Atsubanori: Atsubanori,
Ogonori: Ogonori, Tsurusilamo, Shiramo, Ogogonori, Mizookonori, Kabanori, Beniogonori, Fucresinori, Murasakikabanori, Shinkaikabanori, Togekabanori, Kataogonori, Ryukyuogonori, Ryokyuogonori, Ryukyuogonori, Ryukyuogonori, Ryokyuogonori , Genus Asteraceae: Hachijoenagasomodo, Fushikenori genus: Fushikenori, Namiiwatake genus: Namiiwatake, scorpion genus: Scorpion genus, Occidental genus: Okutsunori, Saimi genus: Itanigusa, Simi, Harigane, Lotus japonicus:
Lotus lotus, genus genus: squid
Nobonori, Black Snapper genus: Black Snapper, Red Snapper genus: Red Snapper, Hishbukuro genus: Hishbukuro, Madaragusa genus: Togemadara, Echimadara, Taoyagusa genus: Taoyagousou, Hanasakura, Saga-no-nagusa , Hirata goat: Hirata goat, Darus: Darus, Masagoshibari, Anadals, Uebagsa: Uebagsa, Benifukuronori: Benifukuronori, Fushitsuna: Fushitsunagi, Himefushinagi,
Species of the genus Hirohafushi and Snapdragons: Snapdragons, Snapdragons, Species: igis, keiigis, crickets, nigiri, amixa, egonori:
Egonori, Futogigis, Saeda: Saeda, Chrysanthemum: Chrysanthemum, Konohanori: Habtaenori, Konohanori, Suzushironori, Usveni: Usveni, Hasuginu: Hasuginu, Nagakonohori: Nagakosugino, Genus suginanu, Hana Yaleusbanori, Sujiusbanori, Hyusbanori, Usubanorimodoki genus: Usubanorimodoki, Ayanishiki: Ayanishiki, Ayaginu:
Ayaginu, Dassia Genus: Enashidazia, Shimadada Genus: Isohagi, Shimadadaji, Dazimodoki Genus: Dazimomodoki, Itogusa Genus: Moletogusa, Futonigusa, Macri Genus: Macri, Salixinus Genus: Hanayanagi, Yuna, Sanagiginori, Mosanyugina, Genus : Kurozozo, Kobusoso, Hanezozo, Sozonohana, Hanegusa: Hanegusa, Kanegusa, Kozanemo: Kozanemo, Isomurasaki, Hosokozanemo, Himegoke: Himegoke, Kurohimegoke, Hyodosigus, Genus genus Suginasugusa, Isobasho Genus: Isobasho, Fujimatsumo Genus: Fujimatsumo, Sawtooth Lilium: Hatakesakinokokihiba, Kawamozuku: Kawamozuku, A Kawamozuku, Himekawamozuku,
Ideyugogome: Ideyugogome, Okitimozuku: Okitimozuku, Itogusa, Chinorimo: Chinorimo, Chisginori: Chisinori, etc.).

Wheel algae (Characta spp.
Hossiturimo: Hossiturimo, Lichnotumnus, Flasks: Himefuramo, Chaboflasmo, Tripella etc., and yellow algae (Hikarimo: Hikarimo etc.).

Materials derived from animal raw materials include a cockscomb extract, a cow or human placenta extract, a pig or cow extract from the stomach, duodenum or intestine or a decomposition product thereof, and a pig or cow spleen extract. Product or its degradation product, extract of pig or cow brain tissue, water-soluble collagen, collagen derivative such as acylated collagen, collagen hydrolyzate, elastin, elastin hydrolyzate, water-soluble elastin derivative, keratin and its decomposition product Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig or bovine blood cell protein degradation product (globin peptide), a pig or bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.),
Milk, casein and its degradation products or their derivatives, skim milk powder and its degradation products or their derivatives, lactoferrin or its degradation products, chicken egg components, fish meat degradation products, nucleic acid-related substances (ribonucleic acid, deoxyribonucleic acid) and the like.

(14) Microbial culture metabolite Yeast metabolite, yeast extract, natto metabolite, natto extract, rice ferment extract, rice bran (red bran, white bran) ferment extract, euglena extract, raw milk or skim milk powder Lactic acid fermentation products, trehalose or derivatives thereof.

(15) α-Hydroxy acids glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) UV absorber / blocker Benzophenone derivatives (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-
5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium salt, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenone-sulfonic acid salt, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), p-aminobenzoic acid Derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate, octyl paradimethylaminobenzoate), methoxycinnamic acid derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate,
Octyl paramethoxycinnamate, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, mono-2-diparamethoxycinnamate
Glyceryl ethylhexanoate, etc.), salicylic acid derivatives (octyl salicylate, salicylphenyl, homomenthyl salicylate, dipropylene glycol salicylate, ethylene glycol salicylate, myristyl salicylate,
Methyl salicylate), anthranilic acid derivatives (methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate, etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives , Camphor derivative, furan derivative, pyrone derivative, nucleic acid derivative, allantoin derivative, nicotinic acid derivative, vitamin B6 derivative, umbelliferone, esculin, benzyl cinnamate, sinoxate, oxybenzone, dioxybenzone, octabenzone, slisobenzone, benzoresorcinol, arbutin Guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin C or its derivatives (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or its derivatives, kojic acid or its derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, placenta extract , Ellagic acid, lucinol and the like.

(19) Tyrosinase activity inhibitor Vitamin C or a derivative thereof (vitamin C phosphate ester magnesium salt, vitamin C glucoside or the like), hydroquinone or a derivative thereof (hydroquinone benzyl ether or the like), kojic acid or a derivative thereof, vitamin E or Derivatives thereof, N-acetyltyrosine or derivatives thereof, glutathione, hydrogen peroxide, zinc peroxide, placental extract, ellagic acid, arbutin, rucinol, silk extract, plant extract (chamomile, mulberry, corn, waremokou, clara, mugwort, Honeysuckle, yellowfin, yellowtail, matsuhodo, adlay, dragonfly, hops, hawthorn, eucalyptus, achillea millefolium, altea, keihi, mankeishi,
Hamamelis, yamaguwa, life-prolonged grass, bellflower, toshishi, spouse, sun-dried, mako, senkyu, dokkatsu, psycho, bohu, hamabou, ogon, peony, peony,
Gennoshoko, kakkon, licorice, five times child, aloe, ginger, safflower, green tea, black tea, asenyaku), etc.

(20) Melanin pigment reduction / decomposition substance Phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide solution, zinc peroxide, hydroquinone or a derivative thereof (hydroquinone benzyl ether)
etc.

(21) Promoting action of turnover / cell activator hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (Aloe, Ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odorikosou, Assemblage, Touki, Tokinsenka, Amacha, Hypericum, Cucumber, Periwinkle,
Rosemary, parsley) etc.

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) etc.

(23) Active oxygen scavengers SOD, catalase, glutathione peroxidase and the like.

(24) Antioxidants Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydroguaselethenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, Parahydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, propolis, etc.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emisoso, Birch, Salvia, Rosemary, Minami Tenji, Agetsu, Ginkgo, Green Tea) and the like.

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor,
dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or a salt thereof, glycyrrhetinic acid or a salt thereof, licorice extract, sicon extract, age extract extract, propolis and the like.

(27) Antibacterial, bactericidal and disinfecting agents acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof, alkyl diaminoethyl glycine chloride solution, triclosan, sodium hypochlorite, Chloramine T, salad powder, iodine compound,
Iodoform, sorbic acid or a salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p-chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, irgasan CH3565, halocarban, hexachlorophen, chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, phenoxyethanol, chlorobutanol, Isopropyl methyl phenol, nonionic surfactants (polyoxyethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene Octyl phenyl ether, etc.), amphoteric surfactants, anionic surfactants (sodium lauryl sulfate, potassium lauroyl sarcosine, etc.),
Cationic surfactants (cetyltrimethylammonium bromide, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride), formaldehyde, hexamine,
Brilliant Green, Malachite Green, Crystal Violet, Jarmal, Photosensitizer No. 101, Photosensitizer No. 201, Photosensitizer No. 401, N-long-chain acyl basic amino acid derivatives and their acid addition salts, zinc oxide, hinokitiol, kudin, propolis, etc. .

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, pyrrolidone carboxylic acid or its salt, lactic acid Sodium, Mini Sasanishiki extract, Bacillus natto metabolite, Bacillus natto extract, Loofah water, Birch (birch) or Akamatsu sap

(29) Inhibitors of elastase activity diisopropyl fluorophosphate, plant extracts (Ougon, Hypericum perforatum, Clara, mulberry leaves, calyx, gennoshoko, comfrey, salvia, elderberry, bollige, buttonpi), seaweed extracts and the like.

(30) Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid and the like.

(31) Antiandrogens Estrogen (eg, estrone, estradiol, ethinylestradiol), isoflavones, oxendrone, etc.

(32) Peripheral vascular blood flow promoter Vitamin E or a derivative thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, touki extract, cepharanthin, carpronium chloride, minoxidil and the like.

(33) Stimulants Pepper tincture, nonyl acid vanillamide, cantaris tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.

(34) Metabolic activators Photosensitizer No. 301, hinokitiol, pantothenic acid or a derivative thereof, allantoin, placental extract, biotin, glyceride pentadecanoate and the like.

(35) Antiseborrheic agent pyridoxine or a derivative thereof, sulfur, vitamin B6
etc.

(36) Keratolytic agent Resorcin, salicylic acid, lactic acid and the like.

(37) Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium acid, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

(38) Hair remover Strontium sulfate, sodium sulfide, barium sulfide,
Inorganic reducing agents such as calcium sulfide; thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, magnesium thioglycolate, strontium thioglycolate) and the like.

(39) Hair swelling agent Ethanolamine, urea, guanidine and the like.

(40) Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3'-iminodiphenol ,
2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, ortho Aminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-
Diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol,
Picramic acid, sodium picramate, N, N'-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenyl Paraphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate,
Orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-sulfate
Diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine , Α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, 2-hydroxy
Sodium 5-5-nitro-2 ', 4'-diaminoazobenzene-5'-sulfonate, hematein and the like.

(41) Flavors Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, Coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Fengqiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batuuri essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Essential oils, vegetable flavors such as Japanese peppermint essential oils, and other synthetic flavors.

(42) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin and the like.

(43) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

(44) Nutrition enhancer Shell calcined calcium, cyanocolabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(45) Dairy products Milk, cheese, fresh cream, butter, margarine, milk powder, whey, condensed milk and the like.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, preservatives / anti-binders, fresheners, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and antiallergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral agents, deodorants / deodorants, bittering agents, seasonings, enzymes, and the like can be used, and when used in combination therewith, various additive and synergistic effects can be expected.

Further, the photoaging protection agent of the present invention, the external preparation for the skin or bath agent, and the dosage form of food and drink are arbitrary, and can be in the form of ampules, capsules, powders, granules, pills, tablets, tablets, etc. Shape, liquid, gel, foam, emulsion, cream, ointment, sheet and other pharmaceuticals, quasi-drugs, skin and hair cosmetics or bath agents, food and drink it can.

Specifically, for example, pharmaceutical preparations for internal and external use, lotions, emulsions, creams, ointments, lotions, oils, packs, and other basic cosmetics, facial cleansers and skin cleansers, shampoos, rinses, and hair treatments , Hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair dyes, hair growth and hair growth products, and other hair cosmetics
Makeup cosmetics such as foundation, white powder, whitening, lipstick, blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc.
Perfumes, bath agents, toothpastes, mouth fresheners / gargles, liquid odor / odor control agents, sanitary articles, sanitary cotton, wet tissues, and the like.

In addition, general foods and drinks, for example, oral compositions such as gums and candies, processed fishery products such as kamaboko and chikuwa, livestock products such as sausage and ham,
Western confectionery, Japanese confectionery, raw noodles, boiled noodles, noodles such as buckwheat, sauce, soy sauce, sauce, sugar, honey, powdered candy,
Seasonings such as water syrup, spices such as curry powder, mustard powder, pepper powder, jam, marmalade, chocolate spread, pickles, soybeans, sprinkle or processed vegetables and fruits such as canned and bottled vegetables and fruits, cheese Dairy products such as butter, yogurt, etc., miso soup, soup, fruit juice, vegetable juice, whey drinks, soft drinks, drinks such as alcoholic beverages), and other beauty foods and health foods.

The photoaging inhibitor containing the extract of Aspergillus niger according to the present invention, a skin external preparation or a bath agent, and a method of adding it to food or drink may be added in advance or during the production. It may be appropriately selected in consideration of workability.

[0086]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Manufacturing Example 1) Japanese red bean, Japanese radish, Japanese gourd, Panax ginseng, Hypericum perforatum, Comfrey,
Salvia, perilla, bollige, and buttonpig 100 g each are extracted with warming in purified water (about 80 ° C) for about 5 hours.
Filter the extract (dry solids: about 0.08-3.0%) to about 1.0
kg.

(Preparation Example 2) [0098] Japanese radish, Chinese cabbage, Japanese gourd, Panax ginseng, Hypericum perforatum, Comfrey,
100 g of each plant of Salvia, Perilla, Perilla, and Bodpi is immersed in a 50% ethanol solution, extracted at room temperature for 5 days and nights, and then filtered to obtain an extract (dry solid content: about 0.1 to
1.0%).

(Production Example 3)
100 g of each plant of Salvia, Perilla, Bodaiju, and Pontopi are immersed in a 40% ethanol solution or a 30% 1,3-butylene glycol solution, or a 30% propylene glycol solution, or purified water (about 30 ° C.), and brought to room temperature. 5 days and nights, and then filter to obtain an extract (dry solids: about 0.1-3.0%).
1.0 kg was obtained.

(Test 1) Photoaging protection test of the extract of Asparagus japonica Thickening of the epidermis was observed in human photoaged skin. Based on this, in the animals observed in this test method, the control group was compared with the group without any UV irradiation.
(Solvent only) showed thickening of the epidermis. From these results, it was determined that the photoaging model could be used. The animals were administered the product of the invention and indomethacin.

(Test Method) a. Sample The extract was extracted 10 times with a 50% aqueous ethanol solution.
did. Extract each of the other plant extracts in the same manner, and
It was mixed with the extract and used as a sample. Positive control is in
Dometasin (manufactured by Sigma) is dissolved in 50% aqueous ethanol solution.
A solution dissolved in 3% was used. In the following formula at the time of application
Was carried out using 10% of each sample. (Ingredients) (% by weight) Triethanolamine 0.4 Potassium hydroxide 0.2 P-210 (Nippon Yushi) 0.3 DOP-8N (Nikko Chemical) 0.3 DDP-10 (Nikko Chemical) 0.1 Polyethylene glycol 1500 2.0 BMG-10 ( Nikko Chemical) 0.5 Dipropylene glycol 0.5 Disodium hydrogen phosphate 0.5 Succinic acid 0.3 Methyl paraben 0.1 Ethanol 10.0 Sample 10.0 Purified water 100.0 b. Test method The method of Kurihara et al. (Kurihara et al., Journal of the Japanese Dermatological Association, 108, 4 (199
8)). That is, a hairless mouse (6 week-old female,
HR-1) 3 times a week on the back (36 to 216 mJ / cm per time) ^Two), Total
4J / cm^TwoUV (Toshiba FL20S ・ E lamp and FL20SBLB
Irradiation was performed for 10 weeks. Each feed
100 μL of the formula applied to the back 1 hour before UV irradiation
did. The animals were divided into 5 animals per group.
Instead, using solvent (50% ethanol aqueous solution), comparison
As an example, a group not irradiated with any ultraviolet light was also provided. In 10 weeks
After the UV irradiation, the animal's skin is collected and a tissue specimen
Was prepared and stained with hematoxylin and eosin.
The average value of each group was calculated.

(Test Results) FIG. 1 shows the thickness of the epidermis of each test group.
It was shown to. When the control group (solvent only) and the non-irradiated group were compared, epidermal hyperplasia of the control group was observed. The epidermal thickness of the indomethacin-administered group showed a tendency to increase in thickness as compared with the non-irradiated group, but decreased compared to the control group. Epidermal thickening was observed in the Kokuboku extract-administered group compared with the non-irradiated group, but the epidermal thickness was clearly reduced as compared with the control group. This reduction was equivalent to the indomethacin treatment group. further,
A decrease in the epidermal thickness was also observed in the sample obtained by combining the Asparagus extract and each plant extract as compared with the control group.

(Test 2) Photo-aging protection test of Asparagus extract The viscoelasticity (plasticity and elasticity) of the photo-aging model animal prepared in Test 1 was measured.

(Measurement Principle) After the ultraviolet irradiation for 10 weeks, the viscoelasticity of the back skin was measured with a skin viscoelasticity meter (CUTOMETER SEM 474 manufactured by Eurotech Japan). Compared. Measurement conditions are Modo
Apply a negative pressure of 500mb for 5 seconds at intervals of 1.5 seconds.
The procedure was repeated twice, and the way in which the skin returned was observed using a waveform, and the average value of each group was calculated.

(Evaluation of Measured Waveform) FIG. 2 shows an example of a skin waveform. The ascending waves were when negative pressure was applied.
The descending wave is when the negative pressure is released. The Uv value indicates a part of the skin viscoelasticity, particularly, plasticity (a property of being continuously deformed when a force is applied and remaining unchanged even when the force is removed). Therefore, in the case of a highly elastic body, since there is almost no plastic portion, the Uv value is extremely small. vice versa,
Mature skin becomes extremely tall. Based on this principle, the degree of plasticity was determined from the following equation. The results are shown in FIG.

<Equation 1> Plasticity = Uv / Ue

The Uf value indicates the maximum suction value, and the return waveform after releasing the negative pressure and completing the suction is indicated by the Ur value. In case of high elasticity,
The waveform when the negative pressure is released immediately drops vertically,
Return to the starting point. Therefore, Uf = Ur. When it becomes a highly viscous body such as skin, two waveforms are observed. An elastic part Ur descending vertically and a viscoelastic plastic part Ua that flattens toward the starting point. If the skin is rich in elasticity, the Uf value and the Ur value will be almost the same. Based on this principle, the elasticity was determined from the following equation. The results are shown in FIG.

<Equation 2> Elasticity = Ur / Uf

(Test Results) From FIGS. 3 and 4, the plasticity of the control group showed an increasing tendency, and the elasticity decreased. The plasticity of the group administered with Koboku was lower than that of the control group, although not as high as indomethacin. Regarding the degree of elasticity, an increase was observed in the Kokuboku extract-administered group compared to the indomethacin-administered group and the control group. Furthermore, also in the sample in which each plant extract was combined, the combined use of the extract of Koboku-ku also showed a decrease in plasticity and an improvement in elasticity.

(Consideration) It has been shown that the plasticity calculated by a skin viscoelasticity measuring device increases with age and the elasticity decreases (Takahashi, Fragrance Journal, 12, 34 (199)
0)). In addition, epidermal thickening has been observed in human photoaged skin (Miyaji, Fragrance Journal, 11, 20 (199
2)). From this, in the animals observed in this test method, the control group containing only the solvent showed an increasing tendency of plasticity, a decrease in elasticity and a thickening of the epidermis in the control group containing no solvent compared with the group not irradiated with any ultraviolet light. The results suggested that it could be a photoaging model.

According to Ota et al. (Ota et al., Fragrance Journal, 4, 25 (1997)), indomethacin was found to have the effect of reducing skin wrinkles and sagging due to ultraviolet rays.
It states that indomethacin can reduce the damage to the skin due to chronic ultraviolet radiation and thus protect against photoaging. It is considered that Koboku which showed the same result as indomethacin in this test method has a photoaging protection effect.

(Test 3) Safety test (1) Primary skin irritation test The extract of Aspergillus niger obtained in Production Examples 1 to 3 was prepared with purified water so that the dry solid concentration was about 1.0%. ,
Japanese white rabbit with shaved back (female, 3 per group, weight 2.
3 kg). Judgment is 24,48,72 after application
Erythema and edema were used as indicators by the primary irritation scoring method at time. The result was negative in all animals without any erythema and edema.

(Test 4) Safety Test (2) Skin Cumulative Irritation Test Similarly, the extract of Aspergillus niger obtained in Production Examples 1 to 3 was purified water so as to have a dry solid concentration of about 1.0%. Once a day, 5 times a week on the skin of Hartley-type guinea pigs (female, 3 animals per group, body weight around 320 g) with shaved flank
Each time, 0.5 mL / animal was applied. The application takes place over two weeks,
Shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. As a result, all animals were judged negative without any erythema and edema over 2 weeks.

(Test 5) Safety Test (3) Acute Toxicity Test A powder obtained by similarly concentrating and drying the extract of Aspergillus niger obtained in Production Examples 1 to 3 (dry solid content: about 1.0
%) Was orally administered to ddy mice (male and female, 5 mice / group, 5 weeks old), which had been fasted for 4 hours before the test, at 2,000 mg / kg, and the appearance and extent of toxic symptoms were observed over time. did. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. Therefore, LD50 is
It was determined to be 2,000 mg / kg or more.

(Prescription examples) Production of various photoaging protection agents or cosmetic compositions The following prescription examples are shown below according to the above evaluation results. Each prescription example is produced by a conventional method in the production of each product. Good, only the amount was shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion Weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Cetyl alcohol 1.5 4. Glycerin monostearate 2.0 5. Polyoxyethylene (20) cetyl ether 3.0 6. Polyoxyethylene (20 ) Sorbitan monooleate 2.0 7.1,3-butylene glycol 1.0 8. Glycerin 2.0 9. Kokuboku extract of Production Example 3 5.0 10. A: Erythmus extract of Production Example 3 B: Ogon extract of Production Example 3 C: Production example Panax ginseng extract of No. 3 D: Hypericum extract of Preparative Example 3 E: Comfrey extract of Preparative Example 3 F: Salvia extract of Preparative Example 3 G: Perilla extract of Preparative Example 3 H: Bodaiju extraction of Preparative Example 3 Liquid I: Red bean extract of Production Example 3 * Extract of one or more of A to I 5.0 11. Preservative (paraoxybenzoic acid ester) qs 12. qsperfume qs 13. Purified water 100

(Formulation Example 2) Emulsion Weight% 1. Squalane 8.0 2. Jojoba oil 7.0 3. Glyceryl paraaminobenzoate 1.0 4. Ethyl urocanate 1.0 5. Magnesium salt of vitamin C phosphate ester 1.0 6. Cetyl alcohol 1.5 7. Glycerin monostearate 2.0 8. Polyoxyethylene (20) cetyl ether 3.0 9. Polyoxyethylene (20) soorbitan monooleate 2.0 10.1,3-butylene glycol 1.0 11. Glycerin 2.0 12. Koboku 60% ethanol extract 3.0 13. Preservative (p-hydroxybenzoate) qs 14. Residual with purified water 100

(Formulation Example 3) Cosmetic oil weight% 1. Liquid paraffin 30.0 2. Squalane 20.0 3. Olive oil 20.0 4. Isopropyl palmitate 10.0 5. Grapefruit fruit or leaf hot water extract 3.0 6. Production example 1 Extract 3.0 3.0 Olive oil 1.0 8. Shea butter 1.0 9. Butyl hydroxyanisole 0.1 10. Vitamin E derivative 0.1 11. Preservative (benzalkonium chloride) qs 12. Fragrance (musk water) qs 13. Purified water 100 Residue

(Formulation Example 4) Flexible lotion% by weight 1. Glycerin 5.0 2.1, 3-butylene glycol 5.0 3. Polyoxyethylene sorbitan monolaurate (20E.O) 1.0 4. Ethanol 15.0 5. Kokuboku 20% ethanol extraction Liquid 1.0 6. A: Extract of E. ginseng extract of Production Example 3 B: Extract of Japanese gourd of Production Example 3 C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I: Perilla extract of Production Example 3 * Extract of any one or more of A to I 8.0 7. 50% ethanol extract of grapefruit fruit or leaf 1.0 8. 50% ethanol extract of kiwi fruit 1.0 9. Appropriate amount of antibacterial and preservative (lactoferrin solution) 10. Appropriate amount of fragrance (Western thin water) 11. Purified water 100 residue

(Formulation Example 5) Astringent lotion weight% 1. Sorvitol 2.0 2. POE (20) oleyl alcohol 1.0 3. Ethanol 15.0 4. Kokuboku 50% 1,3-butylene glycol extract 2.0 5. , 3-butylene glycol extract 2.0 6. Gentian whole plant 50% ethanol extract 1.0 7. Sage whole plant 50% ethanol extract 1.0 8. Allantoin 0.2 9. Astringent (zinc sulfocarbonate) 0.2 10. Antibacterial and preservative (Thiamine lauryl sulfate) Appropriate amount 11.Fragrance (lavender water) Appropriate amount 12.Purified water 100 residue

(Formulation Example 6) Two-layer lotion (liquid to liquid type) wt% 1. squalane 8.0 2. POE sorbitan tetraoleate 0.3 3. sorbit 1.0 4. ethanol 15.0 5. Koboku 45% ethanol extract 2.0 6. Ogon 30% ethanol extract 2.0 7. Comfrey 30% ethanol extract 2.0 8. Aquinome willow 30% ethanol extract 2.0 9. Peony 30% glycerin extract 1.0 10. Extract of hawthorn whole plant 30% glycerin Liquid 1.0 11. Ascorbic acid 0.2 12. Astringent (calamine) 0.1 13. Preservative (phenoxyethanol) proper amount 14. Fragrance (lemon water) proper amount 15. Purified water 100 residue

(Formulation Example 7) Carmine lotion (liquid to solid)% by weight 1. Glycerin 3.0 2.1,3-butylene glycol 5.0 3. Ethanol 15.0 4. Iron oxide (Bengara) 0.2 5. Zinc oxide 0.5 6. Kaolin 2.0 7. Kokuboku 50% propylene glycol extract 2.0 8. Hypericum 50% propylene glycol extract 2.0 9. Panax ginseng root 50% propylene glycol extract 1.0 10. Placenta extract 1.0 11. Camphor 0.2 12. Phenol 0.03 13. Preservative (photosensitive Element 201 & 401) Suitable amount 14.Fragrance (Bodhi tree water) Appropriate amount 15.Purified water 100 residue

(Formulation Example 8) Pack weight% 1. Polyvinyl alcohol 15.0 2. Carboxymethylcellulose sodium 5.0 3. Propylene glycol 3.0 4. Ethanol 10.0 5. Kokuboku 50% ethanol extract 1.0 6. A: Extraction of E. ginseng in Production Example 3 Liquid B: Scutellaria extract of Production Example 3 C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Production Perilla extract of Example 3 H: Bodaiju extract of Production Example 3 I. Pomegranate extract of Production Example 3 * Extract of any one or more of A to I 5.0 7. Thyme whole plant 50% ethanol extract 1.0 8 Melissa leaf or stem 50% ethanol extract 1.0 9. Preservative (paraoxybenzoate) qs 10. Fragrance (Tokisui) qs 11. Purified water 100 balance

(Formulation Example 9) Peel-off pack% by weight 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Pantothenyl ethyl ether 1.0 7. Koboku 50% ethanol Extract 2.0 8. Safflower whole plant 50% ethanol extract 2.0 9. Yuzu fruit 50% ethanol extract 2.0 10. Preservative (paraoxybenzoate) qs 11. perfume (cypress water) qs 12. Purified water 100 Residue

(Formulation Example 10) Cold cream weight% 1. Salicone beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Olive oil 5.0 5. Chamomile oil 5.0 6. Palm oil 5.0 7. Borax 0.5 8. Koboku extract 2.0 (ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 9.Hypericum extract 2.0 (ethanol: 1,3-butylene glycol = 1: 1 extraction solvent) 10.Salvia extract 2.0 (ethanol: 1, 3-butylene glycol = 1: 1 extraction solvent) 11. Liquid shea fat 1.0 12. Preservative (acrinol) 0.1 13. Appropriate amount of fragrance (sage water) 14. Purified water 100 residue

(Formulation Example 11) Emollient cream% by weight 1. Stearic acid 2.0 2. Stearyl alcohol 7.0 3. Reduced lanolin 2.0 4. Squalane 5.0 5. Octyldodecanol 6.0 6. Polyoxyethylene cetyl ether (25E.O.) 3.0 7. Lipophilic glyceryl monostearate 2.0 8. Propylene glycol 5.0 9. Kokuboku hot water extract 3.0 10. Body oil 10% 1,3-butylene glycol extract 3.0 11. Perilla 30% 1,3-butylene glycol extract 3.0 12. Rosemary leaf or flower 50% 1,3-butylene glycol extract 2.0 13. Preservative (paraben) suitable amount 14. Fragrance (cinch water) proper amount 15. Purified water 100 residue

(Formulation Example 12) Face Wash Cream% by Weight 1. Myristic acid 25.0 2. Stearic acid 5.0 3. Tallow fatty acid 5.0 4. Propylene glycol 10.0 5. Potassium hydroxide 6.0 6. Coconut oil fatty acid diethanolamide 6.0 7. Kokuboku extraction Liquid 2.0 (Ethanol: 1,3-butylene glycol = 1: 2 extraction solvent) 8. A: Enmeso extract extract of Production Example 1 B: Ogon extract of Production Example 1 C: Panax ginseng extract of Production Example 1 D: Production Hypericum extract of Example 1 E: Comfrey extract of Production Example 1 F: Salvia extract of Production Example 1 G: Perilla extract of Production Example 1 H: Bodaiju extract of Production Example 1 I: Buttonpig of Production Example 1 Extract * Extract of one or more of A to I 6.0 9. Luffa fruit or stem 50% 1,3-butylene glycol extract 2.0 10. Dipotassium glycyrrhetinate 1.0 11. Preservative (salicylic acid) 0.1 12. Fragrance (Sage ) Remaining to be suitable amount 13. Purified water 100

(Formulation Example 13) Face Wash Cream% by Weight 1. Stearic acid 10.0 2. Palmitic acid 10.0 3. Myristic acid 12.0 4. Lauric acid 4.0 5. Oleyl alcohol 1.5 6. Lanolin derivative (EO adduct) 1.0 7. Glycerin 18.0 8. Potassium hydroxide 6.0 9. Kokuboku 50% 1,3-butylene glycol extract 5.0 10. Royal jelly extract 1.0 (ethanol: 1,3-butylene glycol = 1: 1 extract) 11. Glycyrrhetinic acid 1.0 12. Preservative Agent (salicylic acid) 0.1 13.Perfume (Toki water) Appropriate amount 14.Purified water 100 residue

(Formulation Example 14) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. Whey 1.5 5. Koboku 70% 1,3-butylene glycol extract 1.0 6. A: Antelium extract of Production Example 3 B: Scutellaria extract of Production Example 3 C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Production Example Salvia extract of No. 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I. Persimmon extract of Production Example 3 * Extract of at least one of A to I 5.0 7. Or 70% 1,3-butylene glycol extract 0.5 8. Polyethylene powder 0.5 9. Hydroxypropyl chitosan solution 0.5 10. Amino acids (glycine, glutamine, etc.) 0.5 11. Preservatives (undecylenic acid, phenol) Appropriate quantity 12. pH adjuster Appropriate quantity 13. Perfume (lavender water) Appropriate quantity 14. Purified water 100 residue

(Formulation Example 15) Sunscreen cosmetics (O / W type)% by weight 1. Oxybenzone 2.0 2. Octyl paramethoxycinnamate 5.0 3. Squalane 10.0 4. Vaseline 5.0 5. Stearyl alcohol 3.0 6. Stearic acid 3.0 7. Glyceryl monostearate 2.0 8. Polyethyl acrylate 1.0 9.1,3-butylene glycol 6.0 10. Disodium edetate 0.1 11. Triethanolamine 1.0 12. Titanium dioxide 5.0 13. 50% dipropylene glycol extract in Koboku 0.5 14. A: Extract of E. ginseng extract of Production Example 3 B: Extract of Japanese gourd of Production Example 3 C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I: Perilla extract of Production Example 3 * What is A to I One or more extracts 4.0 15. Honeysuckle whole plant 50% dipropylene glycol extract 0.5 16. Mulberry bark 50% dipropylene glycol extract 0.5 17. Purple root 50% dipropylene glycol extract 0.5 18. Preservative ( Paraben) Appropriate amount 19.Fragrance (apple water) Appropriate amount 20.Purified water 100 residue

(Formulation Example 16) Sunscreen cosmetics (oil type)% by weight 1. Liquid paraffin 70.0 2. Ethyl paraaminobenzoate 1.0 3. Tetrahydroxybenzophenone 1.0 4. Cetyl octanoate 25.0 5. Koboku 30% 1,3 -Butylene glycol extract 1.0 6. Buttonpi 10% 1,3-butylene glycol extract 1.0 7. Aloe leaf 50% 1,3-butylene glycol extract 0.5 8. Saxifrage whole plant 30% 1,3-butylene glycol extract 0.5 9. Barley whole plant 50% 1,3-butylene glycol extract 0.5 10. Fencil fruit 30% 1,3-butylene glycol extract 2.0 11. Antioxidant (butylhydroxytoluene) qs 12. Fragrance (bodaiju water) Appropriate amount

(Formulation Example 17) Cream foundation (O / W type) weight% 1. stearic acid 4.0 2. glycerin monostearate 3.0 3. cetanol 1.5 4. isopropyl myristate 7.0 5. liquid paraffin 10.0 6. salami beeswax 3.0 7. Triethanolamine 1.5 8. Glycerin 3.0 9. Bentonite 1.0 10. Titanium oxide 8.0 11.2,4-Dihydroxybenzophenone 8.0 12. Kaolin 3.0 13. Talc 3.0 14. Color pigment (iron iron oxide) 1.0 15. Hot water extraction Dry powder 0.2 16. Bodaiju hot water extraction dry powder 0.2 17. Preservative (paraben) proper amount 18. Fragrance (rose water) proper amount 19. Purified water 100 residue

(Formulation Example 18) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Kokuboku 20% 2,3-butylene glycol extract 2.0 7. A: Extract of Escherichia coli of Preparation Example 3 B: Extract of Japanese gourd of Preparation Example 3 C: Panax ginseng extract of Preparation Example 3 D: Hypericum perforatum of Preparation Example 3 Extract E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I: Buttonpig extract of Production Example 3 * A Extract of any one or more of I to 5.0 5.0 8. Hydroxypropyl chitosan solution 2.0 9. Mucopolysaccharide solution 1.0 10. Antibacterial and preservative (photosensitive element 101) qs 11. pH control Adjusting agent appropriate amount 12.Perfume (sandalwood water) appropriate amount 13.Purified water 100 residue

(Formulation Example 19) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Koboku 50% 1,2-butylene glycol extract 1.0 6. 50% 1,2-butylene glycol extract 1.0% E. ginseng 1.0 7. Novara 50% 1,2-butylene glycol extract 1.0 8. Futandanpo 50% 1,3-butylene glycol extract 1.0 9. Hydroxyethyl chitosan solution 1.0 10. Trehalose solution 1.0 11. Hydrolyzed silk solution 1.0 12. Appropriate amount of antibacterial and preservative (benzalkonium chloride) 13. Appropriate amount of fragrance (lavender water) 14. Residual amount of purified water 100

(Formulation Example 20) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Ethylene glycol 3.0 6. Kokuboku 50% ethylene glycol extraction Liquid 1.0 7.A: Extract of E. ginseng extract of Production Example 3 B: Extract of Japanese gourd of Production Example 3 C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I: Perilla extract of Production Example 3 * Any one or more of the extracts A to I 5.0 8. Apricot fruit or leaf 30% ethanol extract 1.0 9. Trehalose solution 1.0 10. Hydrolyzed keratin solution 1.0 11. Antibacterial and preservative (benzalkonium chloride) Residual to an appropriate amount 12. Perfume (chamomile water) qs 13. Purified water 100

(Formulation Example 21) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Koboku hot water extract 2.0 6. A : Extract of E. ginseng extract of Production Example 2 B: Extract of Japanese gourd of Production Example 2 C: Panax ginseng extract of Production Example 2 D: Hypericum extract of Production Example 2 E: Comfrey extract of Production Example 2 F: Production Example 2 G: Perilla extract of Production Example 2 H: Bodaiju extract of Production Example 2 I: Perilla extract of Production Example 2 * Extract of one or more of A to I 10.0 7. 50% ethanol extract 1.0 8. Melissa whole plant 50% ethanol extract 1.0 9. Carpronium chloride 0.3 10. Hinokitiol 0.5 11. Keratin decomposition solution 1.0 12. Antibacterial and preservative (parabe , Benzalkonium chloride) qs 13. Perfume (residual to Sinensis water) qs 14. Purified water 100

(Formulation Example 22) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Trimethylene glycol 5.0 6. Koboku 50% Ethanol extract 1.0 7. Egg extract 50% ethanol extract 1.0 8. Parsley whole plant 50% 1,3-butylene glycol extract 0.5 9. Loquat leaf 30% ethanol extract 1.0 10. Azulene 1.0 11. Antibacterial and preservative ( Paraben, benzalkonium chloride) Appropriate amount 12.Fragrance (Touki water) Appropriate amount 13. Residual water to be purified water 100

(Formulation Example 23) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. Koboku 50% ethanol extract 3.0 5. A: Production Example 3 B. Scutellaria extract B of the preparation example 3 C. panax ginseng extract of the preparation example 3 D: Hypericum extract of the preparation example 3 E: Comfrey extract of the preparation example 3 F: Salvia extract of the preparation example 3 G: Perilla extract of Production Example 3 H: Bodaiju extract of Production Example 3 I: Persimmon extract of Production Example 3 * Extract of one or more of A to I 5.0 6. Yuzu leaf or fruit 50% ethanol Extract 3.0 7. Preservative (paraben) qs 8. Purified water 100 residue

(Formulation Example 24) Hair tonic wt% 1.l-menthol 0.2 2. Ethanol 32.5 3. Polyoxyethylene hydrogenated castor oil 0.5 4. Propylene glycol 5.0 5. Diphenhydramine hydrochloride 0.3 6. Koboku hot water extract 2.0 7 Comfrey hot water extract 1.0 8. Panax ginseng 50% ethanol extract 1.0 9. Orange goose 50% ethanol extract 1.0 10. Carboxymethyl chitin solution 1.0 11. Vitamin E derivative 0.5 12. Antibacterial and preservative (paraben) 0.1 13 .Perfume (sage water) Appropriate amount 14. Residual water with 100 purified water

(Formulation Example 25) Hair restorer / hair restorer weight% 1. Ethanol 60.0 2. Kokuboku 50% propylene glycol extract 5.0 3. Panax ginseng root 50% propylene glycol extract 2.0 4. Artemisia leaf or stem 50% 1,3- Butylene glycol extract 1.0 5. 50% nuts 1,3 butylene glycol extract 1.0 6. Vitamin E derivative 0.5 7. Pepper tincture 0.5 8. Resorcin 0.5 9. Dipotassium glycyrrhizinate 0.5 10. Carboxymethyl chitin solution 0.5 11 Beef hematin solution 0.5 12. Mucin solution 0.5 13. Amino acids (glycine, glutamine, etc.) 0.5 14. Antibacterial and preservative (paraben) 0.1 15. Perfume (rosemary water) qs 16. Purified water 100

(Formulation Example 26) Hair restorer / hair restorer% by weight 1. Ethanol 60.0 2. Kokuboku 50% 1,3-butylene glycol extract 1.0 3. A: Extract of E. ginseng of Production Example 3 B: Extract of Japanese gourd of Production Example 3 Liquid C: Panax ginseng extract of Production Example 3 D: Hypericum extract of Production Example 3 E: Comfrey extract of Production Example 3 F: Salvia extract of Production Example 3 G: Perilla extract of Production Example 3 H: Production Bodaiju extract of Example 3 I: Pomegranate extract of Production Example 3 * Extract of one or more of any of A to I 8.0 4. Ethanol extract of whole plant 60% ethanol 5. Canthari tincture 0.5 6. Pantothenic acid Derivatives 0.5 7. Glycyrrhizic acid 0.5 8. Resorcinol 0.5 9. Salicylic acid 0.5 10. Hydroxyethyl chitosan solution 0.5 11. Elastin hydrolyzate 0.5 12. Antibacterial and preservative (paraben) 0.1 13. Perfume (lavender water) Appropriate amount 14. Purification Water 1 00 and the rest

(Formulation Example 27) Granule bath agent weight% 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 2.0 4. Kokuboku hot water extraction dry powder 5.0 5. Sage whole plant hot water extraction dry powder 5.0 6. Suitable amount of pigment (Gunjo green)

(Formulation Example 28) Granule bath agent% by weight 1. Sodium bicarbonate 58.0 2. Anhydrous sodium sulfate 30.0 3. Borax 4.0 4. Kokuboku hot water extraction dry powder 2.0 5. A: Emisoso hot water extraction powder B: Hoton hot water extraction powder C: Panax ginseng hot water extraction powder D: Hypericum hot water extraction powder E: Comfrey hot water extraction powder F: Salvia hot water extraction powder G: Perilla hot water extraction powder H: Bodaiju hot water extraction powder I: Hot-powder hot water extracted powder * Any one or more of A to I 10.0 6. Satsuma mandarin 50% ethanol extracted dry powder 1.0 7. Peach leaf 70% ethanol extracted dry powder 1.0 8. Iris leaf or root 70% ethanol extracted dry powder 1.0 9. Apple juice powder 1.0 10. Pigment (Gunjo, chromium hydroxide) suitable amount 11. Flavor (grape water) proper amount

(Formulation Example 29) Coating protective agent A gauze or liniment cloth is impregnated with a formulation solution containing an appropriate amount of a 50% ethanol extract, an antibiotic, an anti-inflammatory, etc., and attached to the wound. It may be directly applied locally and covered with gauze or the like.

(Formulation Example 30) Mentsuyu liquid% by weight 1. Soy sauce 75.0 2. Black vinegar 2.0 3. Glucose 15.0 4. Sodium glutamate 2.0 5. Kokuboku hot water extraction powder 3.0 6. Comfrey hot water extraction powder 3.0

(Formulation Example 31) Udon or buckwheat wt% 1. Flour or buckwheat flour 96.0 2. Salt 1.0 3. Koboku hot water extract powder 3.0

(Formulation Example 32) Bread Weight% 1. Wheat flour 81.5 2. Salt 0.7 3. Glucose 6.8 4. Kokuboku hot water extraction powder 5.0 5. Panax ginseng hot water extraction powder 3.0 6. Salvia hot water extraction powder 3.0

(Formulation Example 33) Cookie weight% 1. Flour 55.0 2. Milk 8.0 3. Whole egg 14.0 4. Sugar 12.0 5. Constarch 4.6 6. Salt 0.4 7. Kokuboku hot water extraction powder 3.0 8. A: Emmeiso fever Water-extracted powder B: Red gourd hot-water extracted powder C: Panax ginseng hot-water extracted powder D: Hypericum hot-water extracted powder E: Comfrey hot-water extracted powder F: Salvia hot-water extracted powder G: Perilla hot water-extracted powder H: Bodaiju heat Water-extracted powder I: Red bean hot water-extracted powder * Any one or more of A to I 3.0 9. Appropriate perfume

(Formulation Example 34) Sausage weight% 1. Ground meat 89.0 2. Chicken egg 8.0 3. Spices 0.3 4. Seasoning 0.5 5. Koboku hot water extraction powder 1.2 6. Perilla hot water extraction powder 1.0

(Formulation Example 35) Beverage weight% 1. Glucose liquid sugar 33.0 2. Grapefruit juice 50.0 3. Kokuboku hot water extraction powder 2.5 4. A: Emmeso hot water extraction powder B: Ogon hot water extraction powder C: Panax ginseng fever Water extraction powder D: Hypericum hot water extraction powder E: Comfrey hot water extraction powder F: Salvia hot water extraction powder G: Perilla hot water extraction powder H: Bodaiju hot water extraction powder I: Buttonpi hot water extraction powder * A to I 5.0 5. Fragrance 0.5 6. Acidulant proper amount 7. Purified water 100 residue

(Formulation Example 36) Gum weight% 1. Menthol micron 35.0 2. Grapefruit flavor 60.0 3. Kokuboku hot water extraction powder 2.0 4. Emisoe hot water extraction powder 1.5 5. Ogunon hot water extraction powder 1.5

(Test 6) Use effect test A use test was conducted to evaluate the effect when the cosmetic composition of the present invention was actually used. Conditions are for those who suffer from rough or dry skin due to sunburn, or skin without luster or tension.
(20-47 years old, 20 persons) as panelists, an appropriate amount of the emulsion of Formulation Example 1 on the left half of the face after washing the face twice daily in the morning and evening for 3 months, and the active ingredient of the present invention from the emulsion as a control product Is applied to the right half of the face. Here, the test was performed by dividing the test into 10 persons, and one of the tests was performed with Formulation Example 1.
The extract prepared by removing only the extract of Kokuboku from the latex was used, and the other was prepared using the extract of Kokuboku extract. or,
The evaluation method was performed according to the following criteria. The results are as shown in Tables 1 and 2, and the numerical values in the table represent the number of persons. No one complained of abnormal skin during the use period.

"Skin quality improving effect" Effective: Skin gloss and firmness increased, and dry skin quality was improved to fresh skin. Slightly effective: Skin gloss and tension increased slightly, and dry skin quality was slightly improved. Ineffective: No change from before use.

[0144]

[0145]

(Test Results) As shown in Tables 1 and 2, it was confirmed that the emulsion containing the extract of Asparagus of the present invention was effective in increasing the gloss and firmness of the skin and also for the rough and dry skin due to ultraviolet rays. . In addition, together with the extract of Koboku,
A more excellent effect could be confirmed by using together a plant extract selected from a gougon, a panax ginseng, a hypericum, a comfrey, a salvia, a perilla, a borage, and a carp.

(Test 7) Use Effect Test A use test was conducted to evaluate the effect when the food or drink of the present invention was actually used. Conditions are for those who suffer from sunburn with rough skin, dry skin, or shiny skin without tension (20 ~
(Age 47, 20) was a panelist and ate the Cookie of Formulation Example 13 every day for three months. Here, the test was divided into 10 persons, one of which used the cookie of Formulation Example 13 to which only the extract of Koboku was extracted, and the other used the cookie of which the extract of Koboku was extracted. As a control, a cookie from which the active ingredient of the present invention had been removed was used. The evaluation was performed according to the following criteria.
As shown in Table 4 and Table 4, the numbers in the table represent the number of people. No one complained of physical condition during the use period.

"Skin quality improving effect" Effective: The gloss and firmness of the skin increased, and the rough and dry skin quality was improved to a dry skin. Slightly effective: Skin gloss and tension increased slightly, and rough and dry skin quality was slightly improved. Ineffective: No change from before use.

[0149]

[0150]

(Test Results) As shown in Tables 3 and 4, it was confirmed that the cookie containing the extract of Aspergillus niger according to the present invention is effective in increasing the gloss and firmness of the skin, and is also effective for rough skin and dry skin due to ultraviolet rays. . In addition, even more excellent effects could be confirmed by using a plant extract selected from the group consisting of Japanese radish, Japanese gourd, Panax ginseng, St. John's wort, Comfrey, Salvia, Perilla, Bodaiju, and Pontopia, together with the Asparagus extract.

[0152]

EFFECTS OF THE INVENTION A mixture comprising the extract of Asparagus of the present invention and the extract of Aspergillus oryzae, together with at least one plant extract selected from the group consisting of E. glaucoma, Ogon, Panax ginseng, Hypericum perforatum, Comfrey, Salvia, Perilla, Bodaiju, and Botanpi Was found to inhibit epidermal thickening induced by ultraviolet irradiation and to have a photoaging protection effect. In addition, in addition to using the product of the present invention as a photoaging inhibitor, by applying to cosmetic compositions and foods and beverages, it prevents wrinkles and sagging due to ultraviolet irradiation, improves rough and dry skin, and maintains flexibility. Further, it can be used for prevention and treatment of diseases related to the photoaging protection effect. The product of the present invention was also confirmed to be safe for humans and animals.

[Brief description of the drawings]

FIG. 1: Measurement of epidermal thickness after irradiation of a plant extract of Kokuboku extract or a combination of Kokuboku extract with a plant extract such as Japanese cabbage extract, Japanese gourd, Panax ginseng, St. John's wort, Comfrey, Salvia, Perilla, Perilla, Bodaipia It is a figure showing a result.

FIG. 2 is a diagram showing a waveform example of a skin.

FIG. 3 shows the results of measuring the plasticity of the epidermis after irradiation of the extract of the plant of Kokuboku or the mixture of the extract of Kokuboku with the extract of Ginkgo biloba, Japanese gourd, Panax ginseng, St. John's wort, Comfrey, Salvia, Perilla, Perilla, and Bodpi. FIG.

FIG. 4 shows the results of measuring the degree of elasticity of the epidermis after irradiation of a plant extract of Kokuboku extract or a mixture of Kokuboku extract with a plant extract such as Japanese cabbage extract, Japanese gourd, Panax ginseng, Hypericum, Comfrey, Salvia, Perilla, Perilla, and Bodpi. FIG.

────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] February 22, 2001 (2001.2.2)
2)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0023

[Correction method] Change

[Correction contents]

The photoaging protection agent, the cosmetic composition and the food or drink of the present invention may further contain, if necessary, the components and additives described below as long as the effects of the present invention are not impaired. It can be manufactured by optionally selecting and using the agent, the content in the formulation is not particularly defined, but usually 0.0001 ~
50% is preferred.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A23L 1/16 A23L 1/16 F 4B046 1/22 1/22 D 4B047 1/30 1/30 B 4C0831 / 317 1/317 A 4C088 2/52 2/38 C 2/38 A61K 7/06 A61K 7/06 7/075 7/075 7/08 7/08 7/11 7/11 7/42 7/42 7 / 48 7/48 7/50 7/50 35/78 C 35/78 QMW A61P 17/00 A61P 17/00 A23L 2/00 FF term (reference) 4B014 GB13 GG18 GK06 4B017 LC03 LG15 4B018 MD61 MD64 ME10 4B032 DB01 DB22 DK29 4B042 AC04 AD03 AK11 4B046 LA02 LA04 LC07 LG25 4B047 LB09 LG37 LP01 4C083 AA072 AA082 AA111 AA112 AA122 AB032 AB212 AB232 AB242 AB312 AB352 AB372 AB432 AB442 AC022 AC372 AC122 AC122 AC122 AC122 AC122 AC122 AC122 AC122AC122 AC122 AC542 AC582 AC642 AC682 AC692 AC782 AC842 AC852 AC902 AD022 AD092 AD112 AD212 AD322 AD442 AD512 AD532 AD552 AD642 AD662 CC04 CC05 CC06 CC07 CC12 CC19 CC23 CC25 CC32 CC33 CC37 CC38 CC39 DD12 DD22 DD27 DD30 DD31 EE13 EE14 EE17 EE22 4C088 AB12 AC16 AB38 AB05 AC13 CA05 CA06 CA07 CA11 CA13 CA17 MA07 MA52 MA63 NA14 ZA89 ZA92 ZC75

Claims (4)

[Claims] 1. A photoaging protection agent comprising an extract of Asparagus. 2. A photoaging protection comprising: an extract of Aspergillus oryzae, and at least one plant extract selected from the group consisting of Chinese cabbage, Japanese gourd, Panax ginseng, St. John's wort, Comfrey, Salvia, Perilla, Perilla, and Bodpi. Agent. 3. A cosmetic composition comprising the photoaging protection agent according to claim 1 or 2. (4) A food or drink comprising the photoaging protection agent according to (1) or (2).