JP2001098264A - Antioxidant and active-oxygen eliminator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a natural antioxidant and a natural active-oxygen eliminator both of which are excellent in antioxidant action and active-oxygen- eliminating action, are highly safe for human bodies, and can be compounded into cosmetics, drugs, quasi-drugs, food, and various feed. SOLUTION: An antioxidant and an active oxygen eliminator are provided both of which contain, as the effective component, an extract of a plant with a scientific name: Terminalia arborea K. and; V. (indonesian name: Jaha Kling). An oral composition containing the antioxidant or active-oxygen eliminator is also provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a natural product-derived antioxidant and active oxygen scavenger which can be used in the fields of food, cosmetics, medicine, quasi-drugs and feed.

[0002]

2. Description of the Related Art In general, oxygen is indispensable to living organisms, but sometimes it may harm living organisms or affect the quality of foods and cosmetics.

[0003] In particular, active oxygen called superoxide generated by metabolism is reduced by a metal catalyst such as iron or copper to hydrogen peroxide, and further becomes the most reactive hydroxyl radical to cut DNA. ,
It is known that lipids are oxidized to produce lipid peroxide, which is considered as an aging promoting factor.

[0004] These active oxygens are usually produced in vivo in SO
It is eliminated by an enzyme called D (superoxide dismutase), but when the balance of the living body is lost due to various factors such as stress and aging, SOD decreases, lipid peroxide increases due to active oxygen, myocardial infarction, artery Problems such as sclerosis, diabetes, cancer, stroke, cataract, stiff shoulder, chilliness, hypertension, senile dementia and the like, and spots, freckles, wrinkles, etc. have been pointed out.

In articles such as foods, medicines and cosmetics, lipids are peroxidized or polymerized by the influence of oxygen.
Denaturation of the protein, reduction in the activity of the enzyme, fading due to the decomposition of the pigment, and the like occur, which causes deterioration in the quality of foods and the like containing these components.

[0006] As a solution to the inconvenience caused by oxygen, BHA (butylhydroxyaniso-
), BHT (butylhydroxytoluene), α-tocopherol, ascorbic acid, cysteine, glutathione, SOD and other antioxidants and active oxygen scavengers are known.

However, since BHA and BHT are chemically synthesized products, there is concern about human safety. Α-tocopherol and ascorbic acid have relatively excellent active oxygen scavenging effects, It has poor stability, is particularly unstable to heat, and is easily deactivated. In addition to instability, SOD has a problem that it cannot be obtained at a low price due to the difficulty of its preparation.

Under these circumstances, an antioxidant which is safe even if conventionally applied to the human body, has an excellent antioxidant action or active oxygen scavenging action, can be easily produced, and has excellent stability In addition, the development of an active oxygen scavenger has been desired.

[0009]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a naturally occurring antioxidant and an active oxygen scavenger having both an excellent antioxidant action and an active oxygen scavenging action.
Another object of the present invention is to provide an antioxidant and an active oxygen scavenger which are highly safe for the human body and can be incorporated into cosmetics, pharmaceuticals, quasi-drugs, foods and various feeds.

[0010]

Means for Solving the Problems The present inventors have made intensive studies to achieve the above object, and have been used for food (medicine) for many years, and their safety to the human body has been confirmed. It has been found that an extract of a specific plant from Indonesia has high antioxidant activity and is useful as an antioxidant for preventing quality deterioration of cosmetics, pharmaceuticals, quasi-drugs, foods and various feeds. The present inventors have conducted further research based on such findings, and found that the extract has a remarkable antioxidant effect on living bodies (in vivo antioxidant action) based on its excellent antioxidant activity. In other words, it has been found that it has an active oxygen scavenging effect, and various disorders (myocardial infarction,
It is believed that it is useful as an active oxygen scavenger for improving and preventing arteriosclerosis, diabetes, cancer, stroke, cataract, stiff shoulder, chills, hypertension, senile dementia, spots, freckles, wrinkles). The present invention has been completed based on such new findings.

That is, the present invention provides a scientific name: Terminalia a
rborea K. & V. (Indonesia name: Jaha Kling) is an antioxidant containing plant extract as an active ingredient. The present invention is also a composition containing the above plant extract in an effective amount for antioxidant action.

Furthermore, the present invention provides a scientific name: Terminalia arbor
ea K. & V. (Indonesia name: Jaha Kling) An active oxygen scavenger containing plant extracts as active ingredients. The present invention is also an oral composition, particularly a food, containing the above plant extract in an effective amount for eliminating active oxygen.

Further, the present invention provides a scientific name: Terminalia arborea
An oral composition, particularly a food, for preventing active oxygen free radical-induced diseases, comprising an extract of a plant of K. & V. (Indonesia name: Jaha Kling) as an active ingredient.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The scientific name: Terminalia arborea K. & V. (Indonesia name: Jaha Kling) used as a raw material plant of the extract in the present invention has been used as a crude drug in Indonesia since ancient times.

In the present invention, the whole plant of the above plant or a part thereof (for example, roots, stems, leaves, fruits (seed),
Solvent extracts of flower buds, bark, insects, xylem, heartwood, etc.) are used. The plant part used for extraction is not particularly limited as long as it is the whole plant or a part thereof, but a fruit part is particularly preferable. The whole plant or a part thereof of the plant may be subjected to an extraction operation as it is or as a crushed product, or after drying, may be subjected to an extraction operation as a pulverized powder if necessary.

The solvent used for the extraction is not particularly limited, and lower alcohols, polyhydric alcohols, nonpolar solvents and polar solvents can be widely used. More specifically, lower alcohols include alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propanol and isopropyl alcohol, butanol; polyhydric alcohols such as glycerin and polyethylene glycol; non-polar solvents such as pentane; Saturated hydrocarbons such as hexane, heptane, octane, nonane and decane and unsaturated hydrocarbons such as hexene and heptene; water, acetone, ethyl acetate, methyl acetate and the like are used as polar solvents. These solvents may be used alone or in combination of two or more. For example, in the case of a raw material having a high fat content, after a defatting extraction treatment with a non-polar solvent, an extraction treatment may be carried out with various arbitrary solvents, or an extraction treatment using a water-containing organic solvent may be carried out.

As an extraction method, a generally used method can be adopted. Although there is no limitation, for example, a method of immersing whole or part of the plant (as is or coarse powder, finely chopped material) or dried and crushed material (powder, etc.) in a solvent by cold immersion, hot immersion, etc., heating and stirring Extraction and filtration are performed to obtain an extract, or percolation.

The obtained extract is subjected to filtration or centrifugation, if necessary, to remove solids, and then, depending on the mode of use, may be used as it is or may be partially concentrated or dried by distilling off the solvent. May be used. After concentration or drying, the concentrated or dried product may be washed with an insoluble solvent and purified for use, or it may be further dissolved or suspended in a suitable solvent for use. In addition, the extract can be used by obtaining and purifying a fraction having an antioxidant activity and an active oxygen scavenging activity using a conventional purification method, for example, a countercurrent distribution method or liquid chromatography. It is. Furthermore, in the present invention, for example, the solvent extract obtained as described above can be used as a dried plant extract by ordinary means such as drying under reduced pressure and freeze drying.

(1) Antioxidant The antioxidant of the present invention contains the above plant extract as an active ingredient.

The amount of the plant extract contained in the antioxidant can be appropriately changed depending on its use and application.
There is no particular limitation. Further, the plant extract itself can be used as it is as an antioxidant.

The antioxidant of the present invention can be used for foods, quasi-drugs,
It can be added to and used in pharmaceuticals, cosmetics and feeds for the purpose of preventing taste, quality and texture from being reduced due to oxidation of the object, and preventing deterioration, discoloration and burning of oil.

Here, the term "effective amount of antioxidant action" broadly means an amount exhibiting an antioxidant action. Preferably, the components contained in foods, quasi-drugs, pharmaceuticals, cosmetics, feeds, etc. are composed of oxygen. , Heat, light, pH, ion, etc., which can prevent oxidation. The effective amount of antioxidant action varies depending on the type of the target object and the types of components contained therein, and is not particularly limited as long as the above-described effect is exhibited. As a content ratio (in terms of dry matter), it is usually 0.
01 to 30% (% by weight, the same applies hereinafter), preferably 0.0%
A range of 25 to 10% can be exemplified.

Various compositions such as foods, quasi-drugs, pharmaceuticals, cosmetics and feeds containing the antioxidant of the present invention are not particularly limited as long as they can be adversely affected by oxidation.

For example, the foods preferably include fats and oils, foods containing fats and oils, and foods containing pigments (particularly natural pigments).

The fats and oils include corn oil, rapeseed oil, cottonseed oil, soybean oil, safflower oil, sunflower oil, sesame oil, wheat germ oil, olive oil, evening primrose oil, camellia oil, teaseed oil, avocado oil, castor oil , Coffee oil, cashew nut oil, cocoa beans oil, peanut oil, fish oil, palm oil,
Animal kernels such as palm kernel oil, lard, beef tallow, and chicken fat, and partially hydrogenated oils and fats of these animal and plant fats; oleic acid, linoleic acid, α-linoleic acid, γ-linoleic acid, eicosapentaenoic acid, docosahexaenoic acid, etc. Unsaturated fatty acids and their esters or their unsaturated alcohols; and processed fats and oils such as butter, margarine, shortening, and dressings.

As the food containing oils and fats, foods containing a relatively large amount of oils and fats, such as donuts, fried food, fried confectionery, chocolate, instant noodles and the like can be preferably mentioned.

Other foods include Japanese sweets such as Okaki, Senbayashi, Okoshi, Manju, and candy;
Cookies, biscuits, crackers, pies, sponge cakes, castellas, donuts, waffles, pudding, butter cream, custard cream, cream puffs, chocolate, chocolate candy, caramel, candy, chewing gum, jelly, hot cakes, breads and other Western confectionery; potatoes Snacks such as chips; ice cream, ice candy, ice cream such as sherbet; lactic acid drink, lactic acid bacteria drink, concentrated milk drink, fruit juice drink, fruitless drink, pulp drink, functional drink, transparent carbonated drink, carbonated drink with juice, Soft drinks such as fruit-colored carbonated beverages; taste drinks such as green tea, black tea, instant coffee, cocoa, canned coffee drinks, and commercial coffee; dairy products such as fermented milk, processed milk, cheese; soy processed foods such as soy milk ; Marmalai , Jam, fruit syrup pickles; flour paste, peanut paste, paste such as fruit paste; pickles; ham, sausage,
Meat products such as bacon, dry sausage, beef jerky; fish and shellfish products such as fish ham, fish sausage, kamaboko, chikuwa, hampen, tempura and dried fish; Sea urchin,
Salted squid; various delicacies such as dried squid, dried fish mirin, dried shellfish, dried cod, smoked products such as salmon; seaweed, small fish, shellfish, sardines, wild vegetables, mushrooms, kelp, etc .; Curry such as instant curry, retort curry, canned curry; miso, powdered miso, soy sauce, powdered soy sauce, moromi, fish sauce,
Various seasonings such as sauces, ketchup, mayonnaise, solid bouillon, oyster oil, grilled meat sauce, curry roux, stew ingredients, soup ingredients, dash ingredients, etc .; various foods and drinks such as various range foods and frozen foods containing oils and fats Things can be mentioned. The above-mentioned individual foods are examples of foods targeted by the antioxidant of the present invention, and are not limited to these foods.

As for medicines, quasi-drugs, and cosmetics, troches, liver oil drops, mouthwashes, toothpastes, mouth fresheners,
Examples of the agent include an anti-halitosis agent, a sunscreen, a skin lotion, various skin creams, lipsticks, and the like, and examples of the feed include various cat foods, dog foods, bait for ornamental fish, and bait for cultured fish.

The antioxidant of the present invention can be used in combination with other antioxidants, for example, tocopherol, L-ascorbic acid, sodium erythorbate, BHA and the like.

(2) Active oxygen scavenger The active oxygen scavenger of the present invention contains the above-mentioned plant extract as an active ingredient. Like the antioxidant, the amount of the plant extract contained in the active oxygen scavenger can be appropriately changed and adjusted according to its use and application target, and is not particularly limited. Further, the plant extract itself can be used as it is as an active oxygen scavenger.

The active oxygen scavenger of the present invention is used for improving or preventing various disorders that can be caused by active oxygen in a living body, for oral compositions such as foods and pharmaceuticals, and for skins such as cosmetics and external medicines. An effective amount of active oxygen scavenging action can be added to the external preparation and blended for use.

Various disorders that can be caused by the active oxygen in the living body include various inflammations (including gingivitis, periodontal diseases such as periodontal disease, pneumonia, etc.), circulatory diseases such as myocardial infarction and arteriosclerosis. Diabetic complications such as organ diseases, diabetes and cataracts,
Diseases such as various cancers (including, for example, lung cancer and stomach cancer caused by initiators and promoters), stroke, stiff shoulders, chills, hypertension, senile dementia, Alzheimer's dementia due to β-amyloid protein toxicity, and aging ( Active oxygen free radical-induced diseases) and skin disorders such as spots, freckles, and wrinkles are known, but the present invention is not limited to those diseases and skin disorders whose causal relationship with active oxygen is conventionally known. The present invention also covers diseases and the like whose causal relationship with active oxygen is known in the future.

The active oxygen scavenging effective amount means an amount which exhibits an antioxidant effect in a living body or an amount which can reduce or eliminate active oxygen. The effective amount of active oxygen scavenging action varies depending on the application target, the application site, etc., and is not particularly limited as long as the above-mentioned effect is exerted. Specifically, plants in various compositions such as foods, pharmaceuticals, and cosmetics are used. The content of the extract (in terms of dry matter) is usually 0.025 to 30% (% by weight, the same applies hereinafter),
Preferably, a range of 0.05 to 10% can be exemplified.

The active oxygen scavenger of the present invention may be any of various known components (extending agents, excipients, lubricants) depending on the compounding object, as long as the effects of the present invention are not impaired. , Binders, oils, humectants, thickeners, preservatives, emulsifiers,
Chelating agents, pigments, pH adjusters, medicinal ingredients, ultraviolet absorbers, fragrances), and can be used in combination with various foods (eg, health foods), oral administration preparations, external preparations (creams, milk lotions, lotions, Gels, ointments, powders, etc.) and cosmetics (foundations, packs, essences, lip balms, lipsticks, sun care) and the like can be prepared in any form.

The active oxygen scavenger of the present invention can be used in combination with other active oxygen scavengers.

The present invention is particularly directed to an oral composition containing the above active oxygen scavenger. In the present invention, the oral composition broadly includes those that are orally ingested, such as food and drink, orally administered drugs, food additives added to them, and pharmaceutical additives. According to the oral composition, when taken orally, it exerts an antioxidant effect in the living body and can reduce or eliminate active oxygen in the living body, and thus can be caused by the so-called active oxygen free radicals listed above. It is useful for amelioration or prevention of disease.

That is, from such a viewpoint, the present invention provides an oral composition suitably used for preventing a disease caused by active oxygen free radicals. The oral composition may be a food (including a drink) or a pharmaceutical, but is preferably a food. The oral composition can contain various food-sanitary acceptable carriers and additives, or pharmaceutically-acceptable pharmaceutical carriers and additives, depending on the type of the oral composition.

[0038]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Production Examples, Examples, and Test Examples. Production example Scientific name: Terminalia arborea K & V. Terminalia bellerica
Roxb. Dried fruit (Indonesia name Jaha Kling)
To 100 g of the pulverized material, 3 times the amount of hexane was added,
Extracted at room temperature for 24 hours and filtered. A 1-fold amount of hexane was further added to the filtration residue, and the mixture was stirred and filtered to degrease the sample. After drying, the defatted sample was extracted with 5 times the amount of methanol at room temperature for 48 hours. The extract obtained by filtering the extract is concentrated by an evaporator and freeze-dried to obtain a plant extract (hereinafter referred to as Jaha Kling extract) 21.59.
g was obtained.

Reference Production Example 1 Scientific name: 100 g of crushed dried bark of Parameria laevigata (Juss.) Moldenke (Indonesia name: Kayu-rapat)
Then, 1 L of purified water was added thereto, and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract (hereinafter referred to as Kayu-rapat extract).

Reference Production Example 2 Scientific name: Uncaria gambir (Hunter) Roxb. (Indonesia name: Gambir)
1 L of 0 v / v% ethanol was added, and the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract (hereinafter referred to as Gambir extract).

Reference Production Example 3 Scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
1 g of purified water was added to 100 g of the crushed dried fruit of keh), and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract (hereinafter referred to as Cengkeh extract).

Reference Production Example 4 Scientific name: Terminalia belerica Roxb. (Indonesia name: J
1 L of purified water was added to 100 g of the crushed dried pericarp of aha), and the mixture was stirred and extracted on a hot water bath for 1 hour. This was centrifuged and filtered under pressure to obtain an extract (hereinafter referred to as Jaha extract).

Test Example 1 Antioxidant Activity Test The antioxidant activity of the Jaha Kling extract prepared in the above Production Example was determined as follows.
The lipid peroxidation inhibitory ability of the rat liver homogenate was measured as an index. <Method> Rat hepatectomy Rats were fasted overnight, then bled to death and cooled.
The liver was perfused with a% NaCl solution and excised. The obtained liver was homogenized with 1 ml of tissue using 19 ml of 20 mM phosphate buffer under ice-cooling.

Lipid peroxidation reaction of rat liver homogenate 1 ml of the homogenate solution obtained above was placed in a centrifuge tube, and
mM ascorbic acid 10 μl, 10 mM FeSO ₄ 1
Test substance (Jaha Kling) prepared at 0 μl, 5 mg / ml concentration
Extract) (10 μl of methanol solution)
For 1 hour. At this time, the same experiment was performed using 10 μl of methanol as a control. For comparison, a methanol solution of α-tocopherol, a known antioxidant (5 mg / ml) (Comparative product 1), and Kayu-r, which has an antioxidant effect prepared in Reference Production Example 1, are known.
apat extract (methanol solution: 5mg / ml) (Comparative product 2)
Was also similarly tested.

Measurement of lipid peroxide One hour later, 2 ml of TBA reagent (trichloroacetic acid, thiobarbituric acid, 1N hydrochloric acid, BHT, ethanol) was added to the centrifuge tube, and 30 μl of 50 mM BHT ethanol solution was further added. Incubated at 100 ° C. for 15 minutes. Upon completion, the mixture was immediately cooled, centrifuged at 3000 rpm for 10 minutes, the supernatant was transferred to a glass test tube, and the absorbance at 535 nm was measured with a spectrophotometer. The lipid peroxidation inhibition rate (%) was calculated from the obtained absorbance according to the following equation.

[0046]

(Equation 1)

The results are shown in Table 1 together with the results of the comparative experiment.

[0048]

[Table 1]

From these results, it can be seen that the Jaha Kling extract of the present invention has significantly better antioxidant activity than known antioxidants.

Example 2 Free radical scavenging action test The Jaha Kling extract prepared in the above preparation example was subjected to DP
PH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) radical scavenging action was measured by the following method.

First, a 0.1 M acetate buffer solution (PH5.
5) 2 ml of ethanol solution of Jaha Kling extract (2 m
l) and 2 × 10 ⁻⁴ M DPPH ethanol solution (1 m
l) was added to make the total volume 5 ml, and the absorbance at 517 nm was measured 30 minutes later. Using a control system in which 2 ml of ethanol was added instead of the ethanol solution (2 ml) of the Jaha Kling extract as a control, the rate of decrease in absorbance was determined by the following equation. For comparison, α-tocopherol (a reagent manufactured by Nacalai Tesque, purity: 96%) which is a known antioxidant (comparative product 1), and various antioxidant agents prepared in Reference Production Examples 1 to 4 which are known. The same experiment was performed on a plant extract (aqueous solution or methanol solution: 5 mg / ml) (Comparative products 2 to 5).

[0052]

(Equation 2)

Table 2 shows the results. In addition, the result was shown by the test substance amount (mg) required to reduce the absorbance of the control without the test substance to 1/2.

[0054]

[Table 2]

From these results, it can be seen that the Jaha Kling extract of the present invention has a significantly better active oxygen scavenging action than known active oxygen scavengers.

Example 3 Measurement of Superoxide Dismutase (SOD) -Like Activity SOD was measured on the Jaha Kling extract prepared in the above Production Example.
Activity was measured. The SOD-like activity was measured using a Wako Pure Chemicals SOD Test Wako (nitrotetrazolium method) test kit.

Specifically, 1.0 ml of a color developing solution (0.1 M phosphate buffer (pH 8), 0.4 mmol / l xanthine, 0.24 mmol / l nitro blue tetrazolium), 0.1% (W / V)
Put 0.1 ml of JahaKling extract solution in a test tube,
, And 1.0 ml of an enzyme solution (xanthine oxidase 0.049 unit / ml) was added thereto, followed by stirring and then left at 37 ° C. for 20 minutes. After 20 minutes, 2.0 ml of a reaction stop solution (sodium dodecyl sulfate 69 mmol / l) was added, and the absorbance at 560 nm was measured (main test: specimen, E _S ). In addition, a system in which a diluting solvent (0.1 M phosphate buffer (pH 8)) was added instead of the plant extract solution as a sample (this test: blind,
E _BL ), a system to which a blank solution (0.1 M phosphate buffer (pH 8)) was added instead of the enzyme solution (blind: specimen blind, E
_S-BL ), a system in which a diluting solvent was added instead of a sample and a blank solution was added instead of the enzyme solution (blind: reagent blind, E
_BL-BL ), and the SOD activity value (inhibition rate%) was determined according to the following equation. For comparison, various plant extracts of Reference Production Examples 1 to 4 (0.
The same experiment was performed for 0.1 ml of a 1% (W / V) solution (comparative products 2 to 5).

[0058]

(Equation 3)

The measurement procedure is shown in Table 3 below.

[0060]

[Table 3]

Table 4 shows the results.

[0062]

[Table 4]

From these results, it can be seen that the Jaha Kling extract of the present invention has a significantly better active oxygen scavenging action than the known active oxygen scavenger.

[0064]

Formulation Example 2 Sugar-Coated Tablet A mouthwash was prepared by coating 200 parts by weight of a tablet portion with 130 parts by weight of a sugar-coated portion.

[0066]

[0067] Mix and dissolve the plant extract in purified water. Thereafter, sesame oil was added and mixed by stirring to produce a dressing.

[0068] A 50% ethanol solution of polyvinylpyrrolidone is prepared, added to lactose, crystalline cellulose, and corn starch, uniformly mixed, and granulated using a granulator. After drying, the granules were sized to produce granules.

[0069] The above-mentioned raw materials are mixed uniformly, an appropriate amount of a 50% ethanol solution is added, and granulation is performed using a granulator. After drying, it was tableted to produce tablet confections.

[0070]

[0071]

Industrial Applicability The plant extract according to the present invention has excellent antioxidant activity and high safety. Therefore, it can be added to cosmetics, foods, pharmaceuticals, quasi-drugs, feeds, etc. Inferior quality due to oxidation of the various product compositions (fading,
(Deterioration of taste, deterioration, deformation, and deterioration of texture) can be significantly prevented. In addition, the plant extract according to the present invention has an excellent active oxygen scavenging action and high safety,
Incorporation into foods, pharmaceuticals, quasi-drugs, feeds, and other compositions, especially those that are orally ingested, suppresses oxidation in the living body and activates oxygen (free radicals)
Diseases caused by (eg, various inflammations such as gingivitis, periodontitis, pneumonia; aging; cancers caused by initiators and promoters such as gastric cancer and lung cancer; cardiovascular diseases such as arteriosclerosis and myocardial infarction caused by arteriosclerosis; In addition, it is useful for improving or preventing Alzheimer's dementia and diabetic complications (such as cataract) due to the toxicity of β-amyloid protein.
In addition, by being incorporated into cosmetics or external preparations for the skin, it can prevent oxidation of skin lipid components, prevent oxidative damage and aging of the skin, and can improve and prevent skin disorders such as spots, freckles, and wrinkles. .

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61P 43/00 111 A61P 43/00 111 (72) Inventor Ayako Inoki 2-10 East Katayamacho, Suita-shi, Osaka 1 Building 206 (72) Inventor Chika Kato 5-14-37, Nishihara, Kashiwa-shi, Chiba Prefecture (72) Keisuke Sakuta 3-14-6, Naka, Satte-shi, Saitama F-Term (Reference) MF01 4B021 LW03 LW04 LW05 LW06 LW08 MC03 MK05 MP01 MQ04 4C088 AB12 AB99 AC01 AC03 AC04 AC05 AC06 AC09 BA08 BA09 BA10 MA52 NA14 ZA15 ZA33 ZA36 ZA26 ZA42 ZA45 ZA89 ZA96 ZB26 ZC21 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25 ZC25

Claims (6)

[Claims] (1) An antioxidant comprising a plant extract of Terminalia arborea K. & V. (Indonesia name: Jaha Kling) as an active ingredient. 2. A composition comprising an antioxidant effective amount of an extract of a plant of scientific name: Terminalia arborea K. & V. (Indonesia name: Jaha Kling). 3. An active oxygen scavenger comprising, as an active ingredient, a plant extract of Terminalia arborea K. & V. (Indonesia name: Jaha Kling). 4. An oral composition containing an effective amount of an active oxygen scavenging-effective amount of an extract of a plant of scientific name: Terminalia arborea K. & V. (Indonesia name: Jaha Kling). 5. An oral composition for preventing an active oxygen free radical-induced disease by using an extract of a plant of scientific name: Terminalia arborea K. & V. (Indonesia name: Jaha Kling). 6. The oral composition according to claim 4, which is a food.