IL78571A - Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form - Google Patents

Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form

Info

Publication number: IL78571A
Authority: IL; Israel
Prior art keywords: plasminogen activator; human tissue; tissue plasminogen; vectors; enzymatic cleavage
Prior art date: 1985-04-22

Application number

IL78571A

Other languages

English (en)

Other versions

IL78571A0 (en

Original Assignee

Genentech Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-04-22

Filing date

1986-04-21

Publication date

1991-06-30

1986-04-21 Application filed by Genentech Inc filed Critical Genentech Inc

1986-08-31 Publication of IL78571A0 publication Critical patent/IL78571A0/xx

1991-06-30 Publication of IL78571A publication Critical patent/IL78571A/xx

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Crystallography & Structural Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Public Health (AREA)
Diabetes (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Animal Behavior & Ethology (AREA)
Hematology (AREA)
Veterinary Medicine (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Peptides Or Proteins (AREA)

IL78571A 1985-04-22 1986-04-21 Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form IL78571A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US72546885A	1985-04-22	1985-04-22
US84669786A	1986-04-01	1986-04-01

Publications (2)

Publication Number	Publication Date
IL78571A0 IL78571A0 (en)	1986-08-31
IL78571A true IL78571A (en)	1991-06-30

Family

ID=27111150

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL78571A IL78571A (en)	1985-04-22	1986-04-21	Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form

Country Status (21)

Country	Link
US (1)	US5147643A (da)
EP (1)	EP0199574B1 (da)
JP (2)	JPH0740940B2 (da)
AT (1)	ATE68825T1 (da)
AU (1)	AU596356B2 (da)
CA (1)	CA1341580C (da)
DD (1)	DD258026A5 (da)
DE (2)	DE3613390C2 (da)
DK (1)	DK175380B1 (da)
ES (2)	ES8802184A1 (da)
FI (1)	FI98930C (da)
FR (1)	FR2581652B1 (da)
GB (1)	GB2173804B (da)
GR (1)	GR861037B (da)
HU (1)	HU206520B (da)
IE (1)	IE58574B1 (da)
IL (1)	IL78571A (da)
MY (1)	MY102550A (da)
NO (1)	NO175216C (da)
NZ (1)	NZ215920A (da)
PT (1)	PT82429B (da)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS58149871A (ja) *	1982-02-27	1983-09-06	本田技研工業株式会社	二，三輪車の盗難防止装置
JPS6087751A (ja) *	1983-10-20	1985-05-17	House Food Ind Co Ltd	レトルト豆腐の製造法
EP0201153A3 (en) *	1985-02-09	1987-10-07	Beecham Group Plc	Modified enzyme and process for its preparation
ATE80306T1 (de) *	1985-03-06	1992-09-15	Survival Technology	Mittel zur absorptionsverbesserung von proteinen.
US5756093A (en) *	1985-04-22	1998-05-26	Genentech, Inc.	Tissue plasminogen activator variants
US5736134A (en) *	1985-04-22	1998-04-07	Genentech, In.C	Tissue plasminogen activator variants
DD258026A5 (de) *	1985-04-22	1988-07-06	Genentech Inc	Verfahren zur Herstellung von Human-Gewebeplasminogen-Aktivator-Mutanten
US4959318A (en) *	1985-06-27	1990-09-25	Zymogenetics, Inc.	Expression of protein C
US4777043A (en) *	1985-12-17	1988-10-11	Genentech, Inc.	Stabilized human tissue plasminogen activator compositions
EP0227462B1 (en) *	1985-12-23	1992-04-01	Chiron Corporation	Novel peptide plasminogen activators
DK43387A (da) *	1986-01-29	1987-07-30	Beecham Group Plc	Fibrinolytisk enzym
US5837518A (en) *	1986-01-31	1998-11-17	Genetics Institute, Inc.	Thrombolytic proteins
AU612974B2 (en) *	1986-01-31	1991-07-25	Genetics Institute, Llc	Novel thrombolytic proteins
US5071972A (en) *	1986-01-31	1991-12-10	Genetics Institute, Inc.	DNA sequences encoding novel thrombolytic proteins
US5258298A (en) *	1986-01-31	1993-11-02	Genetics Institute, Inc.	Deletion and glycosylation mutant of human tissue plasminogen activator
US5589361A (en) *	1986-03-18	1996-12-31	Genentech, Inc.	Human tissue-type plasminogen activator variant
IE81073B1 (en) *	1986-03-18	2000-01-12	Genentech Inc	Modified human tissue-type plasminogen activator and its preparation
PT84589B (en) *	1986-04-02	1989-05-09	Beecham Group Plc	Process for preparing a fibrinolytic enzyme
PT84588B (en) *	1986-04-02	1989-05-09	Beecham Group Plc	Process for preparing a fibrinolytic enzyme
US5169763A (en) *	1986-04-08	1992-12-08	Transgene S.A., Institut Pasteur	Viral vector coding glycoprotein of HIV-1
GB8701811D0 (en) *	1987-01-28	1987-03-04	Beecham Group Plc	Compounds
MY103358A (en) *	1987-04-15	1993-06-30	Novartis Ag	Process for the production of protiens.
DK275788A (da) *	1987-05-22	1989-02-03	Zymogenetics Inc	Fibrinolytiske proteiner
US5200340A (en) *	1987-05-22	1993-04-06	Zymogenetics, Inc.	Thrombin-activated tissue plasminogen activators
EP0293936B1 (en) *	1987-06-04	1995-02-22	Zymogenetics, Inc.	Tissue plasminogen activator analogs having modified growth factor domains
DK302288A (da) *	1987-06-04	1989-02-03	Zymogenetics Inc	Vaevsplasminogenaktivator med modificeret kringledomaene
DE3886755T3 (de) *	1987-06-30	2005-12-08	Genentech, Inc., South San Francisco	Verfahren zur behandlung von gefässkrankheiten.
US5302390A (en) *	1987-07-01	1994-04-12	Beecham Group Plc	Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) *	1987-08-03	1995-07-31	Fujisawa Pharmaceutical Co	Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
JP2708749B2 (ja) *	1987-08-10	1998-02-04	エーザイ株式会社	修飾型ｔＰＡ含有注射用組成物
US4963357A (en) *	1987-10-09	1990-10-16	Monsanto Company	Tissue plasminogen activator modified by the substitution of Arg for Cys at position 73, methods and pharmaceutical compositions therefor
US5037752A (en) *	1987-10-09	1991-08-06	Monsanto Company	Modified tissue plasminogen activator substituted at cysteine-73 and lysine-277
US5244676A (en) *	1987-10-09	1993-09-14	Monsanto Company	Modified tissue plasminogen activator with modified glycosylation site
DE3851117T2 (de) *	1987-10-16	1995-03-30	Genencor Int	Sequenzspezifische saure E.coli-Protease.
DE3854195T2 (de) *	1987-10-29	1995-12-21	Yamanouchi Pharma Co Ltd	Polypeptid-verbindungen.
US5504001A (en) *	1987-11-25	1996-04-02	Zymogenetics, Inc.	Hybrid plasminogen activator
US5094953A (en) *	1988-03-21	1992-03-10	Genentech, Inc.	Human tissue plasminogen activator variants
WO1990001540A1 (en) *	1988-08-11	1990-02-22	California Biotechnology Inc.	Method for stabilizing heterologous protein expression and vectors for use therein
US5262170A (en) *	1988-09-02	1993-11-16	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5258180A (en) *	1988-09-02	1993-11-02	Genetech, Inc.	Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5108901A (en) *	1988-09-02	1992-04-28	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) *	1988-09-02	1998-02-03	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
US5087572A (en) *	1989-12-01	1992-02-11	Genentech, Inc.	Dna encoding human plasminogen modified at the cleavage site
US5190756A (en) *	1989-12-01	1993-03-02	Genentech, Inc.	Methods and materials for expression of human plasminogen variant
DE3942142A1 (de) *	1989-12-20	1991-06-27	Boehringer Mannheim Gmbh	Stabilisierung von glykosyliertem t-pa
CA2124937C (en) *	1991-12-16	2001-06-05	William F. Bennett	T-pa substitution variants with improved fibrin-specificity
AU670774B2 (en) *	1991-12-16	1996-08-01	Genentech Inc.	Novel tissue plasminogen activator variants
CA2129660C (en) *	1992-06-03	2005-06-28	William F. Bennett	Tissue plasminogen activator glycosylation variants with improved therapeutic properties
GB2298821A (en) *	1995-03-15	1996-09-18	Prestek Ltd	A ribbon winding mechanism
DK0827751T3 (da) *	1996-09-06	2003-03-31	Chemo Sero Therapeut Res Inst	Medicinsk præparat indeholdende vævsplasminogenaktivator og nicotinamid
US6706504B1 (en)	1996-11-12	2004-03-16	The Scripps Research Institute	Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
CA2271697C (en) *	1996-11-12	2007-10-23	The Scripps Research Institute	Tissue type plasminogen activator (t-pa) variants: compositions and methods of use
US5837688A (en) *	1996-11-27	1998-11-17	Gelfand; Mathew I.	Use of thrombolytic reagents for prevention of vascular disease
AU2000268170A1 (en) *	2000-09-04	2002-03-22	Hunan Royal Biotech	A cell line expressing mutant human tissue-type plasminogen activator, constructing strategy and method of expressed protein preparation
US7597895B2 (en) *	2001-02-20	2009-10-06	The Governing Council Of The University Of Toronto	Signal peptides, nucleic acid molecules and methods for treatment of caries
US7556807B2 (en)	2001-02-20	2009-07-07	Kane Biotech, Inc.	Signal peptides, nucleic acid molecules and methods for treatment of caries

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ZA833174B (en) *	1982-05-05	1984-08-29	Genentech Inc	Human tissue plasminogen activator
GR79202B (da) *	1982-05-05	1984-10-22	Genentech Inc
IL90047A (en) *	1982-10-19	1992-12-01	Cetus Oncology Corp	Cysteine-depleted biologically active muteins other than interferon - '
US4753879A (en) *	1984-08-27	1988-06-28	Biogen N.V.	Modified tissue plasminogen activators
EP0201153A3 (en) *	1985-02-09	1987-10-07	Beecham Group Plc	Modified enzyme and process for its preparation
DD258026A5 (de) *	1985-04-22	1988-07-06	Genentech Inc	Verfahren zur Herstellung von Human-Gewebeplasminogen-Aktivator-Mutanten
DK43387A (da) *	1986-01-29	1987-07-30	Beecham Group Plc	Fibrinolytisk enzym
AU612974B2 (en) *	1986-01-31	1991-07-25	Genetics Institute, Llc	Novel thrombolytic proteins

1986
- 1986-04-21 DD DD28944386A patent/DD258026A5/de unknown
- 1986-04-21 AU AU56416/86A patent/AU596356B2/en not_active Expired
- 1986-04-21 DE DE3613390A patent/DE3613390C2/de not_active Expired - Lifetime
- 1986-04-21 FR FR868605756A patent/FR2581652B1/fr not_active Expired
- 1986-04-21 EP EP86302980A patent/EP0199574B1/en not_active Expired - Lifetime
- 1986-04-21 PT PT82429A patent/PT82429B/pt unknown
- 1986-04-21 GR GR861037A patent/GR861037B/el unknown
- 1986-04-21 NO NO861559A patent/NO175216C/no unknown
- 1986-04-21 FI FI861673A patent/FI98930C/fi not_active IP Right Cessation
- 1986-04-21 IL IL78571A patent/IL78571A/xx not_active IP Right Cessation
- 1986-04-21 DE DE8686302980T patent/DE3682104D1/de not_active Expired - Lifetime
- 1986-04-21 HU HU861676A patent/HU206520B/hu unknown
- 1986-04-21 IE IE105586A patent/IE58574B1/en not_active IP Right Cessation
- 1986-04-21 AT AT86302980T patent/ATE68825T1/de not_active IP Right Cessation
- 1986-04-21 GB GB8609683A patent/GB2173804B/en not_active Expired
- 1986-04-21 DK DK198601812A patent/DK175380B1/da not_active IP Right Cessation
- 1986-04-22 ES ES554251A patent/ES8802184A1/es not_active Expired
- 1986-04-22 NZ NZ215920A patent/NZ215920A/xx unknown
- 1986-04-22 JP JP61094382A patent/JPH0740940B2/ja not_active Expired - Lifetime
- 1986-04-22 CA CA000507294A patent/CA1341580C/en not_active Expired - Fee Related
1987
- 1987-09-21 MY MYPI87001800A patent/MY102550A/en unknown
1988
- 1988-01-15 ES ES557800A patent/ES9000011A1/es not_active Expired - Fee Related
1991
- 1991-08-05 US US07/741,120 patent/US5147643A/en not_active Expired - Lifetime
1994
- 1994-02-16 JP JP6019382A patent/JP2529816B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE3682104D1 (de)	1991-11-28
IE58574B1 (en)	1993-10-06
DK181286A (da)	1986-10-23
ES9000011A1 (es)	1990-03-01
ES554251A0 (es)	1988-04-16
DE3613390A1 (de)	1986-10-30
FI98930C (fi)	1997-09-10
PT82429A (en)	1986-05-01
IE861055L (en)	1986-10-22
GB8609683D0 (en)	1986-05-29
MY102550A (en)	1992-07-31
DK181286D0 (da)	1986-04-21
GB2173804B (en)	1989-07-05
ATE68825T1 (de)	1991-11-15
NO175216C (no)	1994-09-14
JP2529816B2 (ja)	1996-09-04
HU206520B (en)	1992-11-30
GR861037B (en)	1986-07-16
EP0199574A3 (en)	1987-08-05
DK175380B1 (da)	2004-09-20
NO175216B (da)	1994-06-06
NZ215920A (en)	1989-07-27
ES557800A0 (es)	1990-03-01
FI861673A (fi)	1986-10-23
NO861559L (no)	1986-10-23
AU5641686A (en)	1986-10-30
IL78571A0 (en)	1986-08-31
JPH06277071A (ja)	1994-10-04
GB2173804A (en)	1986-10-22
HUT40703A (en)	1987-01-28
DE3613390C2 (de)	2001-10-11
US5147643A (en)	1992-09-15
EP0199574A2 (en)	1986-10-29
JPS6224A (ja)	1987-01-06
CA1341580C (en)	2008-09-30
DD258026A5 (de)	1988-07-06
FR2581652B1 (fr)	1989-12-22
ES8802184A1 (es)	1988-04-16
FI98930B (fi)	1997-05-30
JPH0740940B2 (ja)	1995-05-10
AU596356B2 (en)	1990-05-03
EP0199574B1 (en)	1991-10-23
PT82429B (pt)	1988-03-03
FI861673A0 (fi)	1986-04-21
FR2581652A1 (fr)	1986-11-14

Publication	Publication Date	Title
IL78571A (en)	1991-06-30	Human tissue plasminogen activator mutants resistant to enzymatic cleavage resulting in two-chain form
EP0370036B1 (en)	1992-06-03	Modified factor vii/viia
AU572108B2 (en)	1988-05-05	Human tpa production using vectors coding for dhfr protein
ES8205263A1 (es)	1982-06-01	Un procedimiento para la produccion de una proteina activadora del piasminogeno.
GB2176489A (en)	1986-12-31	Polypeptide and polypeptide composition
Sumi et al.	1982	Higher fibrin-binding and thrombolytic properties of single polypeptide chain-high molecular weight urokinase
HUT56884A (en)	1991-10-28	Process for producing protein having urate oxidase activity, gene coding for it, expression vector, microorganisms and transformed cells
US5338546A (en)	1994-08-16	Tissue plasminogen activator variants with decreased clearance
DE3886755T2 (de)	1994-05-26	Verfahren zur behandlung von gefässkrankheiten.
US6706504B1 (en)	2004-03-16	Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
EP0542869B1 (en)	1995-09-13	Tissue plasminogen activator variants with decreased clearance
AU649765B2 (en)	1994-06-02	Tissue plasminogen activator substitution variant
WO1998021320A9 (en)	1998-08-27	TISSUE TYPE PLASMINOGEN ACTIVATOR (t-PA) VARIANTS: COMPOSITIONS AND METHODS OF USE
US5756093A (en)	1998-05-26	Tissue plasminogen activator variants
JPH0330679A (ja)	1991-02-08	ＢａｎＩ制限エンドヌクレアーゼをコードするＤＮＡ

Legal Events

Date	Code	Title
1996-07-23	KB	Patent renewed
2000-08-31	KB	Patent renewed
2004-06-01	KB	Patent renewed
2006-08-01	EXP	Patent expired