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HK1256896A1 - 用於實現多重cold-pcr的方法和組合物 - Google Patents

用於實現多重cold-pcr的方法和組合物

Info

Publication number
HK1256896A1
HK1256896A1 HK18116006.8A HK18116006A HK1256896A1 HK 1256896 A1 HK1256896 A1 HK 1256896A1 HK 18116006 A HK18116006 A HK 18116006A HK 1256896 A1 HK1256896 A1 HK 1256896A1
Authority
HK
Hong Kong
Prior art keywords
pcr
compositions
methods
enable multiplex
cold
Prior art date
Application number
HK18116006.8A
Other languages
English (en)
Inventor
G‧馬克裡吉奧古斯
Original Assignee
達納-法伯癌症研究院有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 達納-法伯癌症研究院有限公司 filed Critical 達納-法伯癌症研究院有限公司
Publication of HK1256896A1 publication Critical patent/HK1256896A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK18116006.8A 2011-03-31 2018-12-13 用於實現多重cold-pcr的方法和組合物 HK1256896A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161470022P 2011-03-31 2011-03-31

Publications (1)

Publication Number Publication Date
HK1256896A1 true HK1256896A1 (zh) 2019-10-04

Family

ID=46932388

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18116006.8A HK1256896A1 (zh) 2011-03-31 2018-12-13 用於實現多重cold-pcr的方法和組合物

Country Status (7)

Country Link
US (1) US11130992B2 (zh)
EP (2) EP2691541B1 (zh)
CA (1) CA2830361C (zh)
DK (1) DK2691541T3 (zh)
ES (1) ES2656557T3 (zh)
HK (1) HK1256896A1 (zh)
WO (1) WO2012135664A2 (zh)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101550489B1 (ko) 2010-03-08 2015-09-07 다나-파버 캔서 인스티튜트 인크. 기준 차단 서열을 이용한 완전 ｃｏｌｄ－ｐｃｒ 풍부화
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
NO2694769T3 (zh) 2012-03-06 2018-03-03
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
RS60032B1 (sr) * 2015-05-18 2020-04-30 Saga Diagnostics Ab Detekcija ciljne nukleinske kiseline i varijanti
ES2917175T3 (es) * 2015-06-24 2022-07-07 Dana Farber Cancer Inst Inc Degradación selectiva de ADN de tipo salvaje y enriquecimiento de alelos mutantes usando nucleasa
WO2017035347A1 (en) * 2015-08-25 2017-03-02 The Broad Institute, Inc. Screening of nucleic acid sequence variations via random loading of optically-encoded particles
WO2017048863A1 (en) * 2015-09-14 2017-03-23 Transgenomic, Inc. Step-up method for ice cold-pcr enrichment
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) * 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
US20210285034A1 (en) * 2018-07-27 2021-09-16 Roche Sequencing Solutions, Inc. Formamide free target enrichment compositions for next-generation sequencing applications
AU2020334071A1 (en) 2019-08-21 2022-01-20 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in DNA microsatellites
EP4126364A4 (en) * 2020-03-24 2024-02-28 Bio-Rad Laboratories, Inc. METHOD AND SYSTEM FOR THERMAL CONTROL OF A CHEMICAL REACTION IN DROPS

Family Cites Families (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB2228086A (en) 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5075217A (en) 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
WO1990013668A1 (en) 1989-05-05 1990-11-15 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
US5256775A (en) 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5045450A (en) 1989-07-13 1991-09-03 Massachusetts Institute Of Technology Determination of a mutational spectrum
DE3938907C2 (de) 1989-11-24 1999-11-04 Dade Behring Marburg Gmbh Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
CA2076853A1 (en) 1990-02-26 1991-08-27 Stephen H. Hughes Method for the fluorescent detection of a dna sequence in real time
WO1991014003A2 (en) 1990-03-09 1991-09-19 Ig Laboratories, Inc. Characterization and analysis of polymorphic vntr loci
CA2116215C (en) 1990-05-07 1998-12-22 Tapestry Pharmaceuticals, Inc. Diagnostic applications of double d-loop formation
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
AU714486B2 (en) 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6010878A (en) 1996-05-20 2000-01-04 Smithkline Beecham Corporation Interleukin-1 β converting enzyme like apoptotic protease-6
US6599704B2 (en) * 1997-03-04 2003-07-29 Christoph Wagener Method for detecting mutated alleles
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
JP3785517B2 (ja) 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6994962B1 (en) * 1998-12-09 2006-02-07 Massachusetts Institute Of Technology Methods of identifying point mutations in a genome
US6850846B2 (en) 2000-01-11 2005-02-01 Affymetrix, Inc. Computer software for genotyping analysis using pattern recognition
DE10012540B4 (de) 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
JP2004520812A (ja) 2000-08-30 2004-07-15 ハプロゲン・エルエルシー 対立遺伝子を決定するための方法
EP1345698A4 (en) 2000-11-16 2006-05-17 Fluidigm Corp MICROFLUID DEVICES FOR THE INTRODUCTION AND DISPOSAL OF FLUIDS FROM MICROFLUID SYSTEMS
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
US6762049B2 (en) 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
DE10158516A1 (de) 2001-11-29 2003-06-12 Focusgenomics Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren
AUPS076902A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Novel selective polymerase chain reaction
AUPS076802A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
JP2005523701A (ja) 2002-04-26 2005-08-11 ユニバーシティ・オブ・ユタ・リサーチ・ファウンデーション 二本鎖核酸−特異的色素を使用する二次構造の融解分析による一本鎖核酸の特徴付け
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
AU2003289651A1 (en) 2002-12-18 2004-07-09 Dingbang Xu Pcr method and application by transnormal low thermo-denaturation temperature
AU2003299817A1 (en) 2002-12-20 2004-07-22 Applera Corporation Genetic polymorphisms associated with stenosis, methods of detection and uses thereof
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
JPWO2006093082A1 (ja) 2005-03-01 2008-08-07 和光純薬工業株式会社 サブトラクション・ポリヌクレオチドの取得方法
US7456281B2 (en) 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
CN101370945B (zh) * 2005-10-14 2011-12-28 克利夫兰州立大学 用于鉴别大量野生型dna背景中多个dna突变标记的方法
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
EP1987162A4 (en) 2006-01-23 2009-11-25 Population Genetics Technologi NUCLEIC ACID ANALYSIS ABOUT SEQUENCE TOKENS
US20100009355A1 (en) 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8501403B2 (en) 2006-03-28 2013-08-06 Commonwealth Scientific And Industrial Research Organisation Amplification of DNA fragments
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
US20080269068A1 (en) * 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2009031054A2 (en) 2007-06-29 2009-03-12 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
CN101815789B (zh) 2007-08-01 2013-06-12 达纳-法伯癌症研究院有限公司 靶序列的富集
US8071338B2 (en) 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
WO2009139898A2 (en) 2008-05-16 2009-11-19 President And Fellows Of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010065626A1 (en) * 2008-12-02 2010-06-10 Globeimmune, Inc. Genotyping tools, methods and kits
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
WO2011011535A2 (en) 2009-07-21 2011-01-27 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
KR101550489B1 (ko) 2010-03-08 2015-09-07 다나-파버 캔서 인스티튜트 인크. 기준 차단 서열을 이용한 완전 ｃｏｌｄ－ｐｃｒ 풍부화
CA2828535A1 (en) 2011-02-28 2012-09-07 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
EA027558B1 (ru) 2011-05-19 2017-08-31 Эйджена Байосайенс, Инк. Способ обнаружения мультиплексных нуклеиновых кислот
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
CN105189746B (zh) 2013-03-14 2018-12-04 宝生物工程株式会社 用于使用耐热的错配内切核酸酶的方法
CN111383715B (zh) 2013-04-17 2024-09-10 先锋国际良种公司 用于在基因组中表征dna序列组成的方法
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture

Also Published As

Publication number Publication date
ES2656557T3 (es) 2018-02-27
WO2012135664A3 (en) 2012-12-27
EP2691541A4 (en) 2015-04-15
WO2012135664A2 (en) 2012-10-04
CA2830361C (en) 2021-03-16
DK2691541T3 (en) 2018-01-22
EP2691541A2 (en) 2014-02-05
EP3333269A1 (en) 2018-06-13
CA2830361A1 (en) 2012-10-04
US11130992B2 (en) 2021-09-28
US20140051087A1 (en) 2014-02-20
EP2691541B1 (en) 2017-10-18
EP3333269B1 (en) 2021-05-05

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