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HK1198169A1 - Method for obtaining fab fragments from single antibody producing cells by multiplexed pcr in combination with taqman probes taqman pcr fab - Google Patents

Method for obtaining fab fragments from single antibody producing cells by multiplexed pcr in combination with taqman probes taqman pcr fab

Info

Publication number
HK1198169A1
HK1198169A1 HK14111645.0A HK14111645A HK1198169A1 HK 1198169 A1 HK1198169 A1 HK 1198169A1 HK 14111645 A HK14111645 A HK 14111645A HK 1198169 A1 HK1198169 A1 HK 1198169A1
Authority
HK
Hong Kong
Prior art keywords
pcr
taqman
fab
combination
producing cells
Prior art date
Application number
HK14111645.0A
Other languages
English (en)
Chinese (zh)
Inventor
Hans-Willi Krell
Alexander Lifke
Valeria Lifke
Kairat Madin
Christian Weilke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of HK1198169A1 publication Critical patent/HK1198169A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
HK14111645.0A 2011-09-21 2014-11-18 Method for obtaining fab fragments from single antibody producing cells by multiplexed pcr in combination with taqman probes taqman pcr fab HK1198169A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11182223 2011-09-21
PCT/EP2012/068532 WO2013041617A1 (fr) 2011-09-21 2012-09-20 Procédé d'obtention de fragments fab à partir de cellules uniques productrices d'anticorps par pcr multiplexe en combinaison avec des sondes taqman

Publications (1)

Publication Number Publication Date
HK1198169A1 true HK1198169A1 (en) 2015-03-13

Family

ID=46852037

Family Applications (1)

Application Number Title Priority Date Filing Date
HK14111645.0A HK1198169A1 (en) 2011-09-21 2014-11-18 Method for obtaining fab fragments from single antibody producing cells by multiplexed pcr in combination with taqman probes taqman pcr fab

Country Status (11)

Country Link
US (1) US20150024434A1 (fr)
EP (1) EP2758428A1 (fr)
JP (1) JP2014527825A (fr)
KR (1) KR20140064857A (fr)
CN (1) CN103814047A (fr)
BR (1) BR112014004566A2 (fr)
CA (1) CA2844838A1 (fr)
HK (1) HK1198169A1 (fr)
MX (1) MX2014003326A (fr)
RU (1) RU2014113678A (fr)
WO (1) WO2013041617A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021226917A1 (fr) * 2020-05-14 2021-11-18 Singleron (Nanjing) Biotechnologies, Ltd. Nouvelle méthode d'amplification du transcriptome entier en une étape

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007089A1 (fr) 1987-03-18 1988-09-22 Medical Research Council Anticorps alteres
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US5571690A (en) 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
GB9613418D0 (en) 1996-06-26 1996-08-28 Crc Technology Ltd A cell-free system for initiation of DNA replication
WO1998031827A1 (fr) 1997-01-21 1998-07-23 Daniel Favre Production de polypeptides biologiquement actifs
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
AU4325900A (en) 1999-03-17 2000-10-04 Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
WO2002013862A2 (fr) 2000-08-11 2002-02-21 Favrille, Inc. Methode et composition de modification d'une pathologie a mediation assuree par les lymphocytes b
AU2004284434A1 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. qRT-PCR assay system for gene expression profiling
PL2121920T3 (pl) * 2007-03-01 2012-01-31 Symphogen As Sposób klonowania pokrewnych przeciwciał
ES2570460T3 (es) * 2009-02-20 2016-05-18 Hoffmann La Roche Método de obtención de ácido nucleico que codifica inmunoglobulina
US20130029340A1 (en) * 2010-01-15 2013-01-31 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an un-specific dye which is not interfering with the emission of fluorophore-labeled probes

Also Published As

Publication number Publication date
EP2758428A1 (fr) 2014-07-30
US20150024434A1 (en) 2015-01-22
CN103814047A (zh) 2014-05-21
BR112014004566A2 (pt) 2017-06-13
RU2014113678A (ru) 2015-10-27
KR20140064857A (ko) 2014-05-28
WO2013041617A1 (fr) 2013-03-28
CA2844838A1 (fr) 2013-03-28
MX2014003326A (es) 2014-04-25
JP2014527825A (ja) 2014-10-23

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