[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

MX2014003326A - Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman. - Google Patents

Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman.

Info

Publication number
MX2014003326A
MX2014003326A MX2014003326A MX2014003326A MX2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A
Authority
MX
Mexico
Prior art keywords
combination
fab fragments
producing cells
antibody producing
single antibody
Prior art date
Application number
MX2014003326A
Other languages
English (en)
Inventor
Valeria Lifke
Hans-Willi Krell
Alexander Lifke
Kairat Madin
Christian Weilke
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014003326A publication Critical patent/MX2014003326A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En la presente se reporta un método para reacción en cadena de polimerasa específica de gen, por transcriptasa inversa en tiempo real en un tubo, multiplexada para amplificación y cuantificación de ácidos nucleicos que codifican para las cadenas pesada y ligera de IgG a fin (isotipo de IgG humano) a partir de una célula única.
MX2014003326A 2011-09-21 2012-09-20 Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman. MX2014003326A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11182223 2011-09-21
PCT/EP2012/068532 WO2013041617A1 (en) 2011-09-21 2012-09-20 METHOD FOR OBTAINING FAB FRAGMENTS FROM SINGLE ANTIBODY PRODUCING CELLS BY MULTIPLEXED PCR IN COMBINATION WITH TaqMan PROBES

Publications (1)

Publication Number Publication Date
MX2014003326A true MX2014003326A (es) 2014-04-25

Family

ID=46852037

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014003326A MX2014003326A (es) 2011-09-21 2012-09-20 Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman.

Country Status (11)

Country Link
US (1) US20150024434A1 (es)
EP (1) EP2758428A1 (es)
JP (1) JP2014527825A (es)
KR (1) KR20140064857A (es)
CN (1) CN103814047A (es)
BR (1) BR112014004566A2 (es)
CA (1) CA2844838A1 (es)
HK (1) HK1198169A1 (es)
MX (1) MX2014003326A (es)
RU (1) RU2014113678A (es)
WO (1) WO2013041617A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021226917A1 (en) * 2020-05-14 2021-11-18 Singleron (Nanjing) Biotechnologies, Ltd. Novel method of one-step whole transcriptome amplification

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007089A1 (en) 1987-03-18 1988-09-22 Medical Research Council Altered antibodies
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US5571690A (en) 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
GB9613418D0 (en) 1996-06-26 1996-08-28 Crc Technology Ltd A cell-free system for initiation of DNA replication
WO1998031827A1 (en) 1997-01-21 1998-07-23 Daniel Favre Production of biologically active polypeptides
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
AU4325900A (en) 1999-03-17 2000-10-04 Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
WO2002013862A2 (en) 2000-08-11 2002-02-21 Favrille, Inc. Method and composition for altering a b cell mediated pathology
AU2004284434A1 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. qRT-PCR assay system for gene expression profiling
PL2121920T3 (pl) * 2007-03-01 2012-01-31 Symphogen As Sposób klonowania pokrewnych przeciwciał
ES2570460T3 (es) * 2009-02-20 2016-05-18 Hoffmann La Roche Método de obtención de ácido nucleico que codifica inmunoglobulina
US20130029340A1 (en) * 2010-01-15 2013-01-31 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an un-specific dye which is not interfering with the emission of fluorophore-labeled probes

Also Published As

Publication number Publication date
EP2758428A1 (en) 2014-07-30
US20150024434A1 (en) 2015-01-22
CN103814047A (zh) 2014-05-21
BR112014004566A2 (pt) 2017-06-13
RU2014113678A (ru) 2015-10-27
KR20140064857A (ko) 2014-05-28
WO2013041617A1 (en) 2013-03-28
CA2844838A1 (en) 2013-03-28
HK1198169A1 (en) 2015-03-13
JP2014527825A (ja) 2014-10-23

Similar Documents

Publication Publication Date Title
HK1245802A1 (zh) 多特異性抗體、抗體類似物、組合物和方法
EP2242836A4 (en) METHOD FOR MANUFACTURING HYBRID CELLS EXPRESSING USEFUL ANTIBODIES
BRPI0916915A2 (pt) anticorpos anti-il-17a/f de reatividade cruzada e biespecíficos
EP2464738A4 (en) METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID
EP2330209A4 (en) METHOD FOR PRODUCING A CONTINUOUS CHEMICAL CULTURE SYSTEM
CL2011000797A1 (es) Metodo para producir anticuerpos a partir de celulas plasmaticas que comprende el cultivo de celulas plasmaticas en cantidades limitadas.
SI2352763T2 (sl) Bispecifična enoverižna protitelesa s specifičnostjo za tarčne antigene z visoko molekulsko maso
UY32501A (es) Anticuerpo antagonista específico para el heterodímero alfa4-beta7
IL224752A (en) METHOD OF USING IL-α1 ANTIBODIES
NZ626269A (en) Anti-phf-tau antibodies and their uses
HK1133905A1 (en) Method for cloning cognate antibodies
EP2633328A4 (en) Automatic real-time pcr system for the various analysis of biological smaple
PL2563739T3 (pl) Sposób wytwarzania cegieł przy zastosowaniu bakterii wytwarzającej enzym
EP2020449A4 (en) METHOD FOR MULTIPLE AND SIMULTANEOUS DETECTION AND QUANTIFICATION OF PATHOGENS BY REAL-TIME POLYMERASE CHAIN REACTION
EP2141497B8 (en) Method for the analysis of circulating antibodies
EP2650375A4 (en) PROCESS FOR THE PRODUCTION OF CHEMICALS BY CONTINUOUS FERMENTATION
EP2115139A4 (en) PROCESS FOR PRODUCING NOVEL IGE-BASED REAGENTS
EP2657343A4 (en) Method for producing chemical by continuous fermentation
WO2012050325A3 (ko) 광생물 반응기
IL206795A0 (en) Multi-step method for producing algae products
MX2014003326A (es) Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman.
WO2009076317A3 (en) Activated t cells
BRPI0922873A2 (pt) método de determinação da temperatura máxima enfrentada por uma rocha sedimentar, especialmente no campo de formação de hidrocarboneto
UA70049U (ru) Способ детекции возбудителя сибирки
EP2484698A4 (en) HYBRIDOMAS FOR THE PRODUCTION OF ANTIBODIES TO METHYLATED DNA AND USE THEREOF