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ES2434853B1 - Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos - Google Patents

Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos Download PDF

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Publication number
ES2434853B1
ES2434853B1 ES201230919A ES201230919A ES2434853B1 ES 2434853 B1 ES2434853 B1 ES 2434853B1 ES 201230919 A ES201230919 A ES 201230919A ES 201230919 A ES201230919 A ES 201230919A ES 2434853 B1 ES2434853 B1 ES 2434853B1
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ES
Spain
Prior art keywords
methods
stem cell
therapeutic
mir
molecular marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn - After Issue
Application number
ES201230919A
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English (en)
Other versions
ES2434853A1 (es
Inventor
Manuel Ángel GONZÁLEZ DE LA PEÑA
Antonio Bernad Miana
María TOMÉ PIZARRO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fundacion Centro Nacional de Investigaciones Cardiovasculares Carlos III
Original Assignee
Fundacion Centro Nacional de Investigaciones Cardiovasculares Carlos III
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Priority to ES201230919A priority Critical patent/ES2434853B1/es
Priority to PCT/ES2013/070381 priority patent/WO2013186418A1/es
Publication of ES2434853A1 publication Critical patent/ES2434853A1/es
Application granted granted Critical
Publication of ES2434853B1 publication Critical patent/ES2434853B1/es
Withdrawn - After Issue legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/178Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Rheumatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos.#La presente invención se relaciona con métodos para determinar la capacidad inmunorreguladora de una célula madre, basándose en los niveles de expresión del microARN miR-335. Adicionalmente, la invención también se relaciona con métodos in vitro para incrementar la capacidad inmunorreguladora de una célula madre, en donde dichos métodos comprenden tratar dicha célula madre con un inhibidor de miR-335. Finalmente, la invención se relaciona con usos y métodos terapéuticos para tratar a un sujeto con una enfermedad autoinmune, una enfermedad inflamatoria, la enfermedad de injerto contra huésped, o una enfermedad que requiera la inducción de tolerancia a un trasplante o la regeneración y/o reparación de tejidos, comprendiendo dichos usos y métodos terapéuticos la administración de una célula madre que presenta niveles reducidos de expresión y/o actividad de miR-335.

Description

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Claims (1)

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ES201230919A 2012-06-12 2012-06-12 Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos Withdrawn - After Issue ES2434853B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201230919A ES2434853B1 (es) 2012-06-12 2012-06-12 Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos
PCT/ES2013/070381 WO2013186418A1 (es) 2012-06-12 2013-06-12 Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201230919A ES2434853B1 (es) 2012-06-12 2012-06-12 Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos

Publications (2)

Publication Number Publication Date
ES2434853A1 ES2434853A1 (es) 2013-12-17
ES2434853B1 true ES2434853B1 (es) 2014-09-30

Family

ID=49034107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201230919A Withdrawn - After Issue ES2434853B1 (es) 2012-06-12 2012-06-12 Marcador molecular de potencia terapéutica de células madre mesenquimales humanas y sus usos

Country Status (2)

Country Link
ES (1) ES2434853B1 (es)
WO (1) WO2013186418A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11096967B2 (en) * 2016-02-26 2021-08-24 Scm Lifescience Co., Ltd. Pharmaceutical composition for preventing or treating regulatory T cell-mediated diseases
CN111979187B (zh) * 2020-08-21 2022-04-08 遵义医科大学附属医院 一种抗人间充质干细胞衰老及增强其干性特征的方法

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* Cited by examiner, † Cited by third party
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US4902505A (en) 1986-07-30 1990-02-20 Alkermes Chimeric peptides for neuropeptide delivery through the blood-brain barrier
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US4904582A (en) 1987-06-11 1990-02-27 Synthetic Genetics Novel amphiphilic nucleic acid conjugates
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5256775A (en) 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
US5264564A (en) 1989-10-24 1993-11-23 Gilead Sciences Oligonucleotide analogs with novel linkages
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6057134A (en) 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6365731B1 (en) 1997-08-06 2002-04-02 Ambion, Inc. Stripping nucleic acids with iodine and sodium thiosulfate
KR20010031140A (ko) 1997-10-14 2001-04-16 루미넥스 코포레이션 정밀 형광염료 입자 및 그의 제조방법 그리고 그의 사용
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6461812B2 (en) 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
US6436640B1 (en) 1999-03-18 2002-08-20 Exiqon A/S Use of LNA in mass spectrometry
US6465183B2 (en) 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
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ATE412184T1 (de) 1999-08-17 2008-11-15 Luminex Corp Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten
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Also Published As

Publication number Publication date
WO2013186418A1 (es) 2013-12-19
WO2013186418A9 (es) 2014-04-17
ES2434853A1 (es) 2013-12-17

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