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ES2150985T3 - Procedimiento y sistema de analisis optico de muestras. - Google Patents

Procedimiento y sistema de analisis optico de muestras.

Info

Publication number
ES2150985T3
ES2150985T3 ES94909763T ES94909763T ES2150985T3 ES 2150985 T3 ES2150985 T3 ES 2150985T3 ES 94909763 T ES94909763 T ES 94909763T ES 94909763 T ES94909763 T ES 94909763T ES 2150985 T3 ES2150985 T3 ES 2150985T3
Authority
ES
Spain
Prior art keywords
analyte
present
density
test
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94909763T
Other languages
English (en)
Inventor
Albert E Chu
Lian Tao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
E-Y Laboratories Inc
Original Assignee
E-Y Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E-Y Laboratories Inc filed Critical E-Y Laboratories Inc
Application granted granted Critical
Publication of ES2150985T3 publication Critical patent/ES2150985T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1765—Method using an image detector and processing of image signal
    • G01N2021/177—Detector of the video camera type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N2021/5915—Processing scan data in densitometry
    • G01N2021/5919—Determining total density of a zone
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N2021/5915—Processing scan data in densitometry
    • G01N2021/5923—Determining zones of density; quantitating spots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N2021/5915—Processing scan data in densitometry
    • G01N2021/593—Correcting from the background density
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N2021/5915—Processing scan data in densitometry
    • G01N2021/5934—Averaging on a zone
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8483—Investigating reagent band
    • G01N2021/8488—Investigating reagent band the band presenting reference patches
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Theoretical Computer Science (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Holo Graphy (AREA)

Abstract

LA PRESENTE INVENCION DEFINE UN METODO Y UN SISTEMA PARA LOCALIZAR Y ANALIZAR AUTOMATICAMENTE UN ANALITO DEPOSITADO SOBRE LA SUPERFICIE DE UN SUBSTRATO DE EXAMEN QUE CONTIENE UN REACTIVO. SE NOTARA VISUALMENTE UN RESULTADO POSITIVO DEL TEST COMO UNA REGION MAS OSCURA DONDE EL ANALITO HA REACCIONADO CON EL REACTIVO. INICIALMENTE, LA PRESENTE INVENCION CALIBRA LA LUZ REQUERIDA PARA DETERMINAR LOS RESULTADOS DEL TEST (124). SE CAPTURA UNA IMAGEN DIGITAL A PARTIR DE LA IMAGEN ANALOGICA (118) Y SE PROCESA (102) MEDIANTE LA PRESENTE INVENCION. LA PRESENTE INVENCION AUTOMATICAMENTE LOCALIZA EL REGION DE INTERES Y COMPUTA UN AREA DE LA MAYOR DENSIDAD DE COLOR U OPTICA CORRESPONDIENTE A LA UBICACION DEL ANALITO. UNA SEGUNDA AREA, LEJOS DEL LUGAR DE REACCION, SE UTILIZA PARA PROPORCIONAR UNA LECTURA DE DENSIDAD DE FONDO. LA DENSIDAD DE FONDO SE UTILIZA ENTONCES PARA AJUSTAR LA MEDICION DE LA DENSIDAD DEL ANALITO SEGUN UNA FUNCION MATEMATICA PREDEFINIDA PARA PROPORCIONAR LOS RESULTADOS CUANTITATIVOS DEL TEST (122).
ES94909763T 1993-02-26 1994-02-24 Procedimiento y sistema de analisis optico de muestras. Expired - Lifetime ES2150985T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2311393A 1993-02-26 1993-02-26

Publications (1)

Publication Number Publication Date
ES2150985T3 true ES2150985T3 (es) 2000-12-16

Family

ID=21813206

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94909763T Expired - Lifetime ES2150985T3 (es) 1993-02-26 1994-02-24 Procedimiento y sistema de analisis optico de muestras.

Country Status (9)

Country Link
US (2) US5717778A (es)
EP (1) EP0731951B1 (es)
JP (1) JP3093270B2 (es)
AT (1) ATE194431T1 (es)
DE (1) DE69425158T2 (es)
DK (1) DK0731951T3 (es)
ES (1) ES2150985T3 (es)
HK (1) HK1014598A1 (es)
WO (1) WO1994019767A1 (es)

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JPH08504950A (ja) 1996-05-28
JP3093270B2 (ja) 2000-10-03
EP0731951A1 (en) 1996-09-18
US5717778A (en) 1998-02-10
WO1994019767A1 (en) 1994-09-01
HK1014598A1 (en) 1999-09-30
EP0731951B1 (en) 2000-07-05
ATE194431T1 (de) 2000-07-15
US6249593B1 (en) 2001-06-19
EP0731951A4 (en) 1997-09-17
DK0731951T3 (da) 2000-11-06
DE69425158T2 (de) 2001-03-15
DE69425158D1 (de) 2000-08-10

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