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EP2647436A2 - Composant de revolver pour un flacon de réactif - Google Patents

Composant de revolver pour un flacon de réactif Download PDF

Info

Publication number
EP2647436A2
EP2647436A2 EP20130155992 EP13155992A EP2647436A2 EP 2647436 A2 EP2647436 A2 EP 2647436A2 EP 20130155992 EP20130155992 EP 20130155992 EP 13155992 A EP13155992 A EP 13155992A EP 2647436 A2 EP2647436 A2 EP 2647436A2
Authority
EP
European Patent Office
Prior art keywords
component
turret
reagent vessel
vessel
breaking point
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP20130155992
Other languages
German (de)
English (en)
Other versions
EP2647436A3 (fr
Inventor
Martina Daub
Arne Kloke
Felix Von Stetten
Nils Paust
Juergen Steigert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of EP2647436A2 publication Critical patent/EP2647436A2/fr
Publication of EP2647436A3 publication Critical patent/EP2647436A3/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber

Definitions

  • the invention relates to a revolver component for a reagent vessel.
  • the invention likewise relates to a reagent vessel insertion part and a reagent vessel.
  • the invention relates to a method for centrifuging a material and to a method for pressure-treating a material.
  • the device constructed in the format of a standard centrifuge tube may comprise various turrets which are arranged axially one above the other.
  • the turrets may include channels, cavities, reaction chambers, and other structures for performing fluidic unit operations.
  • An integrated ballpoint pen mechanism allows the turrets to be rotated with respect to their positions relative to one another, as a result of which the structures of the revolvers can be switched to one another.
  • An update of the ballpoint pen mechanism is triggered after inserting the device in a centrifuge by means of a centrifugal force caused by the operation of the centrifuge. At the same time, liquids can be transferred along the force vector of the centrifugal force produced.
  • the invention provides a revolver component for a reagent container having the features of claim 1, a reagent container insert part having the features of claim 8, a reagent container having the features of claim 11, a method of centrifuging a material having the features of claim 14 and a method for pressure-treating a material having the features of claim 17.
  • the revolver component for a reagent vessel which can be realized by means of the present invention can be emptied in a simple manner on account of the at least one predetermined breaking point formed on the vessel bottom of the at least one vessel structure.
  • Already breaking the at least one predetermined breaking point creates an outlet opening through which the at least one liquid and / or pulverized material filled into the vessel structure can escape.
  • Due to the at least one predetermined breaking point by means of the formation of the at least one predetermined breaking point a material flowing out of the at least one vessel structure or falling material can be collected in a simple manner by means of at least one further vessel structure, for example a further turret component.
  • the present invention offers a simpler removal of this compared to a conventional suction of at least one filled into the at least one vessel structure material.
  • the at least one predetermined breaking point of the turret component according to the invention is advantageous, since in such a predetermined breaking point less licking of the at least one vessel structure filled at least one material is to be feared ,
  • leaking spots often occur in the case of a covering film, through which an undesired escape of the at least one filled-in material can take place.
  • the conventionally often required process step for covering continuous cavities of a turret component by means of a foil in the manufacture of the turret component according to the invention can be saved.
  • the conventionally practiced sometimes forming a double bottom in a prior art turret which has been covered with the foil may be omitted in the manufacture of the turret member of the present invention.
  • the turret component according to the invention is thus produced with a simplified geometry, since no double bottom, in particular for an implementation of a switching logic, is needed.
  • the revolver component is manufactured in one piece by means of a casting process or an injection molding process.
  • the turret component according to the invention can be produced inexpensively by means of a simple executable process.
  • the at least one predetermined breaking point frames at least one cover element partially so that the respective cover element can be deflected out of a plane of the associated predetermined breaking point after breaking the assigned predetermined breaking point about a predetermined bending point.
  • a suitable outlet opening for discharging the at least one material filled in the at least one vessel structure can be reliably realized in a sufficient size.
  • the breaking of the predetermined breaking point leads to a fall of a material previously covering the outlet opening of the turret component into a further vessel structure which traps the outgoing material.
  • the revolver component has a revolver outer wall, which is designed such that the revolver component can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • the revolver component can also be used in an insertion part housing of a reagent vessel insertion part, which is designed such that the reagent vessel insertion part can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • the turret component according to the invention can thus be used for controlling a chemical reaction and / or a biochemical / molecular biological process during centrifuging a material, applying an overpressure and / or applying a negative pressure.
  • the turret component according to the invention is thus suitable for a variety of uses.
  • the at least one predetermined breaking point can be designed such that the predetermined breaking point can be effected by means of a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged with the revolving component inserted therein, and / or by means of a centrifugal force during operation Druckvariiervoriques, in which the reagent vessel is arranged with the turret component inserted therein, frictional effect frictional effect.
  • a preferred refractive power can be set reliably by the formation of the predetermined breaking point in a simple manner.
  • the at least one predetermined breaking point can be broken even by effecting a centrifugal force / or a compressive force equal to the specified refractive power.
  • a centrifugal force / or a compressive force equal to the specified refractive power.
  • an advantageous / preferred time for breaking the at least one predetermined breaking point in a simple manner by a corresponding predetermining the speed, the negative pressure and / or the pressure can be adjusted.
  • the revolver component on and / or in the at least one vessel structure may have at least one holding device, by means of which at least one mass part with a retention force and / or in the at least one holding device is durable, wherein the at least one holding device to the at least one predetermined breaking point is arranged, that after releasing the restraining force, the at least one released mass part falls on the at least one predetermined breaking point and / or on at least one of the at least one predetermined breaking point at least partially framed bottom surface.
  • the cancellation of the restraining force can be reliably carried out, for example, by means of a centrifugal force produced during the operation of the centrifuge and / or by means of a pressure force exerted during the operation of the pressure-varying device.
  • a desired / advantageous time for the discharge of the material filled in the at least one vessel structure can be firmly maintained by means of a specification of the rotational speed, the negative pressure and / or the overpressure.
  • a reagent vessel insert having an insert housing adapted to employ the reagent vessel insert in a reagent vessel for a centrifuge and / or pressure varying device and at least one disposed within the insert housing Revolver component according to the invention feasible.
  • the reagent vessel insertion part in addition to a first turret component with the at least one predetermined breaking point, also comprises at least one second turret component, wherein the first turret component and the second turret component Revolver component are arranged by means of an elastic spacer component to each other so that the first turret member and the second turret component by means of a centrifugal force effected in an operation of the centrifuge, in whose rotor means the reagent vessel is arranged with the reagent vessel inserting part inserted therein, and / or by means of a at Operation of the Druckvariiervoriques, in which the reagent vessel is arranged with the therein Reagenzgefäß-insert part, effecting compressive force can be brought into contact with each other so that on the contact, a refractive power to the at least one predetermined breaking point is transferable.
  • the second turret component is already in a position relative to the first turret component, in which a reliable collection of emerging from the first turret component by means of the second turret component is ensured.
  • the reagent vessel insertion part may include a ballpoint pen mechanism as the elastic spacer component.
  • a low-cost mechanism for the elastic spacer component can be used.
  • Fig. 1a and 1b show a plan view and a cross section of an embodiment of the revolver component.
  • Revolver component 10 shown schematically can be used in a reagent vessel.
  • the revolver component 10 may have a turret outer wall 12, which is designed so that the revolver component 10 can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device.
  • the turret component 10 may be insertable in an insert part housing of a reagent vessel insert which is adapted for insertion of the reagent vessel insert into a reagent vessel for a centrifuge and / or pressure varying device.
  • the applicability of the revolver component 10 / of the reagent vessel insert into the relevant reagent vessel for a centrifuge and / or a pressure varying device can be interpreted such that the revolver outer wall 12 / an outer wall of the Einassieilgephaseuses to an inner wall of the reagent vessel corresponds.
  • the turret outer wall 12 / the outer wall of the Einassieilgephaseuses contacted the inner wall of the reagent vessel such that even during operation of the centrifuge and / or the Druckvariiervoriques a reliable hold of the turret member 10 / the Reagenzgefäß insertion is ensured in the relevant reagent vessel.
  • the reagent vessel can be understood to mean a (standard) test tube / test tube.
  • Other embodiments include centrifuge tubes, 1.5 ml Eppendorf tubes, 2 ml Eppendorf tubes, 5 ml Eppendorf tubes and microtiter plates, such as e.g. 20 ⁇ L microtiter plates (per well).
  • the reagent vessel can be a test carrier or a disposable cartridge, which are designed as a lab-on-a-chip system on a plastic-plastic-sized plastic substrate.
  • the formability of the reagent vessel is not limited to the examples listed here.
  • the dimensions of the reagent vessel are predetermined only due to a desired usability of the reagent vessel in the centrifuge and / or in the Druckvariiervoriques. However, the feasibility of the technologies according to the invention described below does not prescribe any external form of the reagent vessel.
  • the reagent vessel can be designed to receive samples in an amount which can be chosen optionally from a range of a few ⁇ L up to 1 L.
  • the technology according to the invention can be used by means of any centrifuge, by means of which a (minimum) centrifugal force can be exerted from 20 g.
  • the technology according to the invention can be used for any pressure varying device, by means of which a sub and / or overpressure can be applied.
  • Under the revolver component 10 can be understood in particular a turret for a reagent vessel.
  • the turret component 10 may be designed, for example, such that it can be rotated about an axis of rotation 11 by means of a suitable mechanism which can be arranged on the turret component 10 or separately from the turret component 10.
  • the axis of rotation 11 may, in particular, run centrally through the revolver component 10 and / or be aligned perpendicular to the at least one vessel bottom.
  • the revolver component 10 / the reagent vessel insertion part can also be designed for cooperation with a ballpoint pen mechanism, or a ballpoint pen mechanism include.
  • the turret member 10 / reagent vial insert may hold a volume less than 5 milliliters.
  • the revolver component 10 can thus be designed in particular such that it can be integrated in a stack of further revolvers and / or reaction chambers.
  • turrets, reaction chambers and / or cavities axially stacked one above the other
  • azimuthally relative to one another With regard to a possible execution of the ballpoint pen mechanism is on the DE 2010 003 223 A1 directed.
  • At least one vessel structure 14, into which at least one liquid and / or pulverized material 16 can be filled, is formed on the revolver component 10.
  • the revolver component 10 has at least one predetermined breaking point 20 on at least one vessel bottom 18 of the at least one vessel structure 14.
  • at least one outlet opening can thus be created, through which the material 16 filled into the at least one vessel structure 14 can flow out / fall out.
  • the filled into the at least one vessel structure 14 material 16 can be easily removed again.
  • a desired / advantageous time for the exit of the material 16 can also be set.
  • At least one predetermined breaking point 20 frames at least one cover element 22 partially so that the respective cover element 22 can be bent out of a plane of the associated predetermined breaking point 20 after breaking the associated predetermined breaking point 20 about a predetermined bending point 24.
  • a hinge component and / or a suspension can also be used.
  • the at least one predetermined breaking point 20 can in particular be designed in such a way that the at least one predetermined breaking point 20 can be brought about by means of a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged with the revolving component 10 inserted therein, and / or by means of a centrifugal force during operation Druckvariiervoriques, in which the reagent vessel is arranged with the turret component 10 inserted therein, frictional effecting frictional effect.
  • the preferred for breaking the at least one predetermined breaking point 20 time by means of a correspondingly effecting the centrifugal force and / or the pressing force can be set reliably.
  • the at least one predetermined breaking point 20 may for example be designed so that the at least one predetermined breaking point 20 breaks due to a centrifugal force which is exerted by the material 16 stored in the vessel structure 14. This is the case if the centrifugal force exceeds the mechanical stability of the predetermined breaking point 20 above a threshold value, which is typically greater than 20 g.
  • a threshold value which is typically greater than 20 g.
  • the at least one predetermined breaking point 20 can be broken at an adjustable / definable time.
  • the at least one predetermined breaking point 20 is also breakable without a direct breaking of the at least one predetermined breaking point 20 by means of the centrifugal force and / or the pressure force at a desired time.
  • the turret member 10 may be made in one piece by means of a casting method or an injection molding method despite its advantageous usability.
  • the turret component 10 is thus inexpensive to produce.
  • the internal volume of the turret member 10 / reagent vial insert may be at least partially made of a polymer, e.g. from COP, COC, PC, PA, PU, PP, PET and / or PMMA. Other materials are also suitable for forming the interior volume of the turret member 10 / reagent vial insert.
  • At least one channel, at least one cavity and / or at least one reaction chamber may be formed in the revolver component 10 / a reagent vessel insertion part equipped therewith.
  • Process steps and structures, such as, for example, sedimentation structures, channel structures or siphon structures for forwarding and switching at least one liquid contained in the revolver component 10 / the reagent vessel insertion part can be integrated in the inner volume of the revolver component 10 / of the reagent vessel insertion part.
  • At least one further subunit of the inner volume of the revolver component 10 / of the reagent vessel insertion part can be filled with at least one liquid as a "storage container" which contains at least one chemical reaction and / or with a subsequently filled, to be processed and / or examined material / sample material performs a biochemical / molecular biological process.
  • the at least one "storage container” can eg with chemicals (eg buffers), enzymes, lyphilisates, beads, dyes, antibodies, antigens, receptors, proteins, DNA strands and / or Be filled with RNA strands.
  • the turret member 10 / reagent vial insert may also be equipped with additional components such as valves and / or pumps.
  • the technology according to the invention can also interact with a multiplicity of conventional actuation, detection and / or control units.
  • Fig. 2a and 2 B show a cross section and a partial cross section of a first embodiment of a Reagenzgefäß-inserting part.
  • the reagent vessel insertion part 30, shown diagrammatically, has an insertion part housing 32, which is designed such that the reagent vessel insertion part 30 can be inserted in a reagent vessel for a centrifuge and / or for a pressure variation device.
  • the applicability of the reagent vessel insertion part 32 into the relevant reagent vessel for a centrifuge and / or a pressure varying device can be interpreted such that an outer wall 34 of the insertion part housing 32 corresponds to an inner wall of the reagent vessel.
  • the outer wall 34 of the Einiganeilgephases 32 contacts the inner wall of the reagent vessel such that even during operation of the centrifuge and / or the Druckvariiervorraum a reliable hold of the Reagenzgefäß insertion part 30 is ensured in the relevant reagent vessel.
  • the reagent vessel into which the reagent vessel insertion part 30 can be inserted reference is made to the above-enumerated embodiments.
  • the reagent vessel cooperating with the reagent vessel insertion part 30 is not limited to these.
  • the reagent vessel insertion part 30 includes at least one turret member 10a, 10b, and 10c disposed in the insertion part housing 32.
  • the at least one revolver component 10a, 10b and 10c can be designed so that it can be rotated about the axis of rotation 11.
  • the at least one turret component 10a, 10b and 10c can also be adjustable along the axis of rotation 11 (lateral). As will be explained in more detail below, a distance between adjacent turret components 10a, 10b and 10c can also be varied in this way.
  • the lateral adjustability of the at least one turret component 10a, 10b and 10c is, for example, by means of a ballpoint pen mechanism 36, which in FIG Fig. 2a only is shown schematically, feasible.
  • a ballpoint pen mechanism 36 which in FIG Fig. 2a only is shown schematically, feasible.
  • a deformable polymer / elastomer can be used to provide a restoring force, which returns the at least one Revolver components 10a, 10b and 10c in a predetermined starting position / initial position causes.
  • a compressible material such as a polymer, can be used for this purpose.
  • a compressible material instead of a compressible material, it is also possible to use a stretchable material which generates a tensile force which, as the restoring force, causes the at least one turret component 10a, 10b and 10c to be returned to a starting position / starting position.
  • the reagent vessel insertion part 30 has, in addition to a first turret component 10a with the at least one predetermined breaking point 20, at least one second turret component 10b.
  • a centrifugal force and / or pressure force can be exerted as actuation force Fa on the at least one predetermined breaking point 20, that this breaks.
  • a material 16 filled into the at least one vessel structure 14 of the first turret component 10a can be transferred to a further vessel structure 38 of the second turret component 10b.
  • the second turret member 10b is aligned with respect to the first turret member 10a in the direction of the actuator force Fa.
  • the reaction vessel insertion part 30 is therefore particularly suitable for swing-bucket rotors.
  • the actuation force Fa may also be used to actuate the ballpoint pen mechanism 36 or a similar component.
  • Fig. 3 shows a schematic partial view of a second embodiment of the Reagenzgefäß insertion part.
  • This in Fig. 3 partially re-introduced reaction vessel insertion part 30 has at least one turret component 10a with at least one predetermined breaking point 20, wherein the turret component 10a and / or in the at least one vessel structure 14 has at least one holding device 40, by means of which at least one mass portion 42 with a retaining force on and / or in the at least one holding device 40 is durable.
  • the at least one holding device 40 is also arranged to the at least one predetermined breaking point 20, that after releasing the restraining force, the at least one released mass portion 42 on the at least one predetermined breaking point 20 and / or at least one of the at least one predetermined breaking point 20 at least partially framed bottom surface For example, the lid member 22 falls.
  • the at least one mass part 42 thus acts as an actuation element for breaking the at least one predetermined breaking point 20.
  • the cancellation of the retaining force can be effected in particular by means of a centrifugal force and / or a compressive force during operation of a centrifuge and / or a pressure varying device.
  • the time for canceling the restraining force which triggers an acceleration of the mass part 42 along a movement path 44 oriented in the direction of the actuation force Fa can also be advantageously set.
  • the at least one predetermined breaking point 20 may be designed so that it does not break even at a centrifugal acceleration of about 10000g, or at a corresponding compressive force, and despite the mass of filled in the at least one vessel structure 14 material 16 and is stable ,
  • the mass portion 42 can be released and accelerated by means of the actuation force Fa so that it breaks when breaking on or at the predetermined breaking point 20.
  • a retaining device 40 for example, a magnet, in particular a permanent magnet, are used, which extracts the at least partially formed of a magnetically attractable material mass portion 42.
  • the holding device 40 may be a mechanically biased holding device 40, in which the mass portion 42 is inserted so that it dissolves from a force equal to the retaining force of the mechanically biased holding device 40.
  • the holding device 40 may be an actively controllable actuator which can be formed, for example, by means of an actively activatable magnet or a coil. The advantageous position of the at least one holding device 40 can be ensured by attaching it to a cover element 46 / cover.
  • the examples of forming and arranging the retainer 40 and mass portion 42 described herein are to be interpreted as exemplary only.
  • Fig. 4 shows a schematic partial view of a third embodiment of the Reagenzgefäß-inserting part.
  • Partially schematically reproduced reagent vessel insertion part 30 comprises at least the first turret component 10a with the at least one predetermined breaking point 20 and the second turret component 10b.
  • the first turret component 10a and the second turret component 10b are arranged relative to one another by means of an elastic spacer component 48 such that the first turret component 10a and the second turret component 10b use an actuation force Fa, such as during operation of a centrifuge, in the rotor device of the reagent vessel arranged therein inserted reagent vessel insertion part 30, effecting centrifugal force and / or by means of a in a operation of a Druckvariiervoriques, in which the reagent vessel is arranged with the Reagenzgefäß-inserting member 30, cause compressive force, so that they can be brought into contact with each other over the contact a refractive power to the at least one predetermined breaking point 20 is transferable.
  • the elastic spacer component 48
  • the embodiment described here ensures the above-mentioned advantages.
  • a break of at least one predetermined breaking point 20 only takes place when the contact between the two turret components 10a and 10b is present.
  • the liquid emerging from the at least one vessel structure 14 of the first turret component 10a can be reliably collected by means of the second turret component 10b.
  • a highlight 50 is formed.
  • the highlighting 50 preferably extends along the direction of the actuation force Fa, for example radially outward and / or inward Direction to the second turret component 10b.
  • the highlighting 50 may be formed, for example, as an elevation, as a bridge and / or as a pin.
  • a reduction in the (lateral) distance between the two turret components 10a and 10b by means of the actuation force Fa results in contact between the highlight 50 and the second turret component 10b.
  • the highlight 50 may contact the second turret member 10b, for example, from a centrifugal acceleration of 20 g.
  • a further increase in the actuation force Fa for example due to an increase in the centrifugal acceleration, can lead to a breakage of the respective predetermined breaking point 20.
  • the cover element 22 can be pressed into the associated vessel structure 14 due to the contact between the highlighting 50 and the second turret component 10b. In this way, the material 16 introduced into the associated vessel structure 14 can be quickly released.
  • the highlighting 50 preferably contacts the second turret component 10b at a contact surface 52 which lies within the further vessel structure 38 of the second turret component 10b.
  • the contact surface 52 can lie on a step 54 formed in the further vessel structure 38 or a corresponding support structure / emphasis.
  • stage 54 e.g. Also, a web or a pin in the further vessel structure 38 may be formed. In this way, it can be ensured that the breaking of the assigned predetermined breaking point 20 does not take place until the outlet opening exposed in this way is advantageously positioned relative to the further vessel structure 38.
  • Fig. 5 shows a schematic partial view of a fourth embodiment of the Reagenzgefäß-inserting part.
  • the emphasis 50 is attached to a mounting surface 56 of the second turret component 10b.
  • the attachment surface 56 is preferably located within a further vessel structure 38 of the second turret component 10b, for example on a step 54 or on a corresponding support structure / emphasis. (Under the support structure, a web or a pin can be understood.)
  • Fig. 6 shows a schematic partial view of a fifth embodiment of the Reagenzgefäß-inserting part.
  • the geometry of a contact side 58 of the first turret component 10a aligned with the second turret component 10b is designed such that the contact side 58 can dip into the at least one further vessel structure 38 of the second turret component 10b.
  • the emphasis 50 attached to the first turret component 10a is designed so short that the breaking of the assigned predetermined breaking point 20 takes place only after a partial immersion of the first turret component 10a into the second turret component 10b.
  • the material 16 filled into the at least one vessel structure 14 can be transferred free of leakage from the at least one vessel structure 14 of the first revolver component 10a into at least one further vessel structure 38 of the second revolver component 10b.
  • the advantageous plug-socket principle of the embodiment of Fig. 6 is formed by the surfaces of the turret components 10a and 10b are not only designed planar and parallel to each other, but provided with elevations and depressions, which are partially offset, complementary and / or overlapping each other.
  • Fig. 7a to 7d show schematic partial views of a sixth embodiment of the Reagenzgefäß insert part.
  • a second turret component 10b at least two further vessel structures 38-1 and 38-2 each have a protruding from a bottom surface 62 of the other vessel structure 38 web element 60-1 and 60-2.
  • a first web element 60-1 of a first vessel structure 38-1 of the second turret component 10b is arranged in a first distance a1 directed radially away from the rotation axis 11 to a side inner wall 64-1 of the first vessel structure 38-1 which extends radially from the axis of rotation 11 directed away second distance a2 in which a second web element 60-2 of a second vessel structure 38-2 of the second turret component 10b is arranged to a side inner wall 64-2 of the second vessel structure 38-2.
  • Under the side inner walls 64-1 and 64-2 side wall surfaces can be understood on an outer wall protruding from the bottom surface 62, wherein the outer wall can be aligned in particular (almost) parallel to the axis of rotation 11.
  • the second turret component 10b also comprises at least one third vessel structure
  • its web element can be arranged in a third distance directed radially away from the rotation axis 11 to a side inner wall of the third vessel structure which is not equal to the distances a1 and a2 may thus be equal to the number of vessel structures of the second turret component 10b.
  • each of the at least two vessel structures 38-1 and 38-2 of the second turret component 10b is assigned a respective vascular structure 14-1 and 14-2 of the first turret component 10a.
  • the respective vessel structure 14-1 and 14-2 of the first turret component 10a has an emphasis 50-1 and 50-2, which in a radially away from the rotation axis 11 directed away distance a1 or a2 to an adjacent to the contact side 58 edge 66 of a Side outer wall 68 of the first turret member 10a on the contact side 58 is arranged.
  • a first emphasis 50-1 of a first vessel structure 14-1 of the first turret component 10a which is assigned to the first vessel structure 38-1 of the second turret component 10b, is thus in the radial direction away from the rotation axis 11 first distance a1 to the edge 66 of Side outer wall 68 of the first turret member 10a disposed on the contact side 58.
  • a second emphasis 50-2 of a second vessel structure 14-2 of the first turret component 10a which is assigned to the second vessel structure 38-1 of the second turret component 10b, in the second distance a2 directed radially away from the rotation axis 11 to the edge 66 of FIG Side outer wall 68 of the first turret member 10a disposed on the contact side 58.
  • a switching logic can be realized by means of which a first material 16-1 filled into the first vessel structure 14-1 of the first turret component 10a and a different second material 16-2 filled into the second vessel structure 14-2 of the first turret component 10a are selectively / purposefully in certain vascular structures 38-1 and 38-2 of the second turret component 10b are transferable.
  • Fig. 7a shows a rotational position of the two turret components 10a and 10b to each other, in which the vessel structures 14-1 and 14-2 of the first turret member 10a to a Angle of rotation ⁇ not equal to zero with respect to their associated vessel structures 38-1 and 38-2 of the second turret component 10b are rotated.
  • the non-zero rotational angle ⁇ is in Fig.
  • the distance is shown as a distance.
  • the first turret member 10a partially plunges into the second turret member 10b, however, each of the vessel structures 14-1 and 14-2 of the first turret component 10a immersed in a unassigned vessel structure 38-1 and 38-2 of the second turret component 10b (see Fig. 7b ).
  • the first vessel structure 14-1 of the first turret component 10a dips, for example, into the second vessel structure 38-1 of the second turret component 10b.
  • the first highlight 50-1 of the first vessel structure 14-1 of the first turret member 10a along its dip path 69-1 does not strike the second support structure 60-2 of the second vial structure 38-2 of the second turret member 10b.
  • the second highlight 50-2 of the second vessel structure 14-2 of the first turret member 10a does not contact the first bearing structure 60-1 of the first vessel structure 38-1 of the second turret member 10b along its dip path 69-2.
  • the difference between the distances a1 and a2 is thus selected to be sufficiently large to allow contact between the first prominence 50-1 of the first vessel structure 14-1 of the first revolving member 10a and the second abutment structure 60-2 of the second vessel structure 38-1 of the second revolving member 10b, despite the immersion of the prevent first vessel structure 14-1 of the first turret member 10a in the second vessel structure 38-1 of the second turret member 10b.
  • first turret component 10a on the side outer wall 68 on a diameter extension which is formed for example as an external step 70.
  • the space defined by the side inner walls 64-1 and 64-2 of the second turret component 10b has a maximum extent perpendicular to the axis of rotation which is smaller than the maximum diameter of the side outer wall 68 of the first turret component 10a.
  • the second turret component 10b thus exerts no force on the predetermined breaking points 20 of the first turret component 10a, despite the immersion of the first vessel structure 14-1 of the first turret component 10a into the second vessel structure 38-2 of the second turret component 10b.
  • the two turret parts 10a and 10b are then by means of a Force Fk of a (not outlined) ballpoint pen mechanism pulled apart again.
  • a renewed tightening of the two turret components 10a and 10b thus leads to immersion of the first vessel structure 14-1 of the first turret component 10a into the first vessel structure 38-1 of the second turret component 10b and to immersion of the second vessel structure 14 -2 of the first turret component 10a into the second vessel structure 38-2 of the second turret component 10b.
  • the emphasis 50-1 and 50-2 respectively contact their associated support structures 60-1 and 60-2, so that the predetermined breaking points 20 are broken and material outlet openings in the vessel structures 14-1 and 14-2 of the first turret component 10a are opened.
  • the various materials 16-1 and 16-2 can thus be filled in a targeted manner into their assigned vessel structures 38-1 and 38-2 of the second turret component 10b.
  • a vessel structure 38-1 and 38-2 of the second turret component 10b can also have a plurality of support structures 60-1 and 60-2 at different positions.
  • the vessel structure 38-1 and 38-2 of the second turret member 10b may be coupled to a plurality of vessel structures 14-1 and 14-2 of the first turret member 10a.
  • the further vessel structure 38-1 and 38-2 of the second turret component 10b can thus be used advantageously as a mixing and / or incubation chamber.
  • a vessel structure 14-1 and 14-2 of the first turret member 10a may have a plurality of highlights 50-1 and 50-2 at different positions.
  • a reagent vessel insertion part according to the technology according to the invention also apply to a reagent vessel for a centrifuge and / or a pressure varying device, which is designed in accordance with the described reagent vessel insertion parts.
  • the advantageous reagent vessel has an outer wall, which is formed so that the reagent vessel in a centrifuge and / or in a Druckvariiervoriques can be used.
  • the reagent vessel is designed so that a reliable hold of the reagent vessel is ensured in the operated centrifuge and / or in the operated Druckvariiervorraum.
  • a reagent vessel for a centrifuge and / or a pressure variegating device can thus be understood to mean a reagent vessel which, due to its (external) shape, lends itself well to operation of the centrifuge with a comparatively high rotational speed and / or application of a superordinate to the atmospheric pressure - And / or negative pressure by means of Druckvariiervoriques.
  • the advantageous reagent vessel may have vessel structures, such as channels, reaction chambers, storage chambers and / or active components, such as valves and / or pumps.
  • the reaction vessel may comprise actuation, detection and control units.
  • the advantageous reagent vessel has at least one turret component arranged in the reagent vessel with at least one predetermined breaking point.
  • the reagent vessel may also comprise at least one second turret component, wherein the first turret component and the second turret component are arranged relative to each other by means of a resilient spacer component such that the first turret component and the second turret component by means of a Operation of a centrifuge, in the rotor device, the reagent vessel is arranged, effecting centrifugal force and / or by means of a force in an operation of a Druckvariiervoriques in which the reagent vessel, be effected compressive force can be brought into contact with each other so that a contact force on the at least one predetermined breaking point is transferable.
  • the reagent vessel may include a ballpoint pen mechanism as the resilient spacer component.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Centrifugal Separators (AREA)
EP20130155992 2012-04-04 2013-02-20 Composant de revolver pour un flacon de réactif Withdrawn EP2647436A3 (fr)

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DE102012205516A DE102012205516A1 (de) 2012-04-04 2012-04-04 Revolverbauteil für ein Reagenzgefäß

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Cited By (1)

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USD903898S1 (en) 2018-09-24 2020-12-01 Gentueri Inc. Sampling assembly
US11471889B2 (en) 2018-09-24 2022-10-18 Gentueri Inc. Sample assembly
CN110302850B (zh) * 2019-06-10 2024-08-23 东莞东阳光医疗智能器件研发有限公司 一种离心式液体释放装置
CN110508338B (zh) * 2019-08-30 2024-07-02 烟台芥子生物技术有限公司 用于微流控芯片的液囊

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JP6041800B2 (ja) * 2011-04-25 2016-12-14 富士紡ホールディングス株式会社 試薬容器

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2926906A1 (fr) * 2014-04-04 2015-10-07 Robert Bosch Gmbh Dispositif de soupape et dispositif de manipulation de liquides

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CN103357521A (zh) 2013-10-23
DE102012205516A1 (de) 2013-10-10
EP2647436A3 (fr) 2015-03-04

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