EP2647436A2 - Revolver component for a reagent container - Google Patents

Abstract

The component (10) has a vessel structure (14) formed on the turret component to receive liquid and/or pulverized material (16). A predetermined breaking point (20) is formed at a vessel base (18) of the vessel structure. The predetermined breaking point partially frames a cover element (22) such that the cover element is bendable out of a plane of the breaking point about a predetermined bending point (24) after the breaking point breaks. A turret outer wall (12) enables insertion of the turret component in a reagent vessel. Independent claims are also included for the following: (1) a method for centrifuging a material (2) a method for the pressure treatment of a material (3) a reagent vessel for a centrifuge and/or for a pressure varying apparatus (4) a reagent vessel insertion part.

Description

The invention relates to a revolver component for a reagent vessel. The invention likewise relates to a reagent vessel insertion part and a reagent vessel. Furthermore, the invention relates to a method for centrifuging a material and to a method for pressure-treating a material.

State of the art

In the DE 10 2010 003 223 A1 a device for insertion into a rotor of a centrifuge is described. The device constructed in the format of a standard centrifuge tube may comprise various turrets which are arranged axially one above the other. The turrets may include channels, cavities, reaction chambers, and other structures for performing fluidic unit operations. An integrated ballpoint pen mechanism allows the turrets to be rotated with respect to their positions relative to one another, as a result of which the structures of the revolvers can be switched to one another. An update of the ballpoint pen mechanism is triggered after inserting the device in a centrifuge by means of a centrifugal force caused by the operation of the centrifuge. At the same time, liquids can be transferred along the force vector of the centrifugal force produced.

Disclosure of the invention

The invention provides a revolver component for a reagent container having the features of claim 1, a reagent container insert part having the features of claim 8, a reagent container having the features of claim 11, a method of centrifuging a material having the features of claim 14 and a method for pressure-treating a material having the features of claim 17.

Advantages of the invention

The revolver component for a reagent vessel which can be realized by means of the present invention can be emptied in a simple manner on account of the at least one predetermined breaking point formed on the vessel bottom of the at least one vessel structure. Already breaking the at least one predetermined breaking point creates an outlet opening through which the at least one liquid and / or pulverized material filled into the vessel structure can escape. Due to the at least one predetermined breaking point by means of the formation of the at least one predetermined breaking point, a material flowing out of the at least one vessel structure or falling material can be collected in a simple manner by means of at least one further vessel structure, for example a further turret component.

Thus, the present invention offers a simpler removal of this compared to a conventional suction of at least one filled into the at least one vessel structure material. Even with respect to the conventional manner often applied covering the at least one bottom surface of the at least one vessel structure by means of a foil, the at least one predetermined breaking point of the turret component according to the invention is advantageous, since in such a predetermined breaking point less licking of the at least one vessel structure filled at least one material is to be feared , In contrast, leaking spots often occur in the case of a covering film, through which an undesired escape of the at least one filled-in material can take place.

Furthermore, it should be noted that in the manufacture of the turret component according to the invention no sealing of a capacity / vessel structure by means of a foil, such as a sealing foil made of aluminum, must be performed. Thus, the conventionally often required process step for covering continuous cavities of a turret component by means of a foil in the manufacture of the turret component according to the invention can be saved. Similarly, the conventional way often necessary training a thorn structure on a lid of a downstream turret, which is often required according to the prior art, to pierce the film. Also, the conventionally practiced sometimes forming a double bottom in a prior art turret which has been covered with the foil may be omitted in the manufacture of the turret member of the present invention. The turret component according to the invention is thus produced with a simplified geometry, since no double bottom, in particular for an implementation of a switching logic, is needed.

Preferably, the revolver component is manufactured in one piece by means of a casting process or an injection molding process. Thus, the turret component according to the invention can be produced inexpensively by means of a simple executable process.

In an advantageous embodiment, the at least one predetermined breaking point frames at least one cover element partially so that the respective cover element can be deflected out of a plane of the associated predetermined breaking point after breaking the assigned predetermined breaking point about a predetermined bending point. In this way, a suitable outlet opening for discharging the at least one material filled in the at least one vessel structure can be reliably realized in a sufficient size. In addition, by means of the formation of the at least one predetermined bending point, it can be prevented that the breaking of the predetermined breaking point leads to a fall of a material previously covering the outlet opening of the turret component into a further vessel structure which traps the outgoing material.

In a further advantageous embodiment, the revolver component has a revolver outer wall, which is designed such that the revolver component can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device. As an alternative or in addition thereto, the revolver component can also be used in an insertion part housing of a reagent vessel insertion part, which is designed such that the reagent vessel insertion part can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device. The turret component according to the invention can thus be used for controlling a chemical reaction and / or a biochemical / molecular biological process during centrifuging a material, applying an overpressure and / or applying a negative pressure. The turret component according to the invention is thus suitable for a variety of uses.

For example, the at least one predetermined breaking point can be designed such that the predetermined breaking point can be effected by means of a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged with the revolving component inserted therein, and / or by means of a centrifugal force during operation Druckvariiervorrichtung, in which the reagent vessel is arranged with the turret component inserted therein, frictional effect frictional effect. In particular, a preferred refractive power can be set reliably by the formation of the predetermined breaking point in a simple manner. In this case, even by effecting a centrifugal force / or a compressive force equal to the specified refractive power, the at least one predetermined breaking point can be broken. Thus, during operation of the centrifuge and / or the Druckvariiervorrichtung an advantageous / preferred time for breaking the at least one predetermined breaking point in a simple manner by a corresponding predetermining the speed, the negative pressure and / or the pressure can be adjusted.

Furthermore, the revolver component on and / or in the at least one vessel structure may have at least one holding device, by means of which at least one mass part with a retention force and / or in the at least one holding device is durable, wherein the at least one holding device to the at least one predetermined breaking point is arranged, that after releasing the restraining force, the at least one released mass part falls on the at least one predetermined breaking point and / or on at least one of the at least one predetermined breaking point at least partially framed bottom surface. The cancellation of the restraining force can be reliably carried out, for example, by means of a centrifugal force produced during the operation of the centrifuge and / or by means of a pressure force exerted during the operation of the pressure-varying device. Thus, also in this embodiment, a desired / advantageous time for the discharge of the material filled in the at least one vessel structure can be firmly maintained by means of a specification of the rotational speed, the negative pressure and / or the overpressure.

The advantages enumerated in the preceding paragraphs are also provided by means of a reagent vessel insert having an insert housing adapted to employ the reagent vessel insert in a reagent vessel for a centrifuge and / or pressure varying device and at least one disposed within the insert housing Revolver component according to the invention feasible.

In an advantageous embodiment, in addition to a first turret component with the at least one predetermined breaking point, the reagent vessel insertion part also comprises at least one second turret component, wherein the first turret component and the second turret component Revolver component are arranged by means of an elastic spacer component to each other so that the first turret member and the second turret component by means of a centrifugal force effected in an operation of the centrifuge, in whose rotor means the reagent vessel is arranged with the reagent vessel inserting part inserted therein, and / or by means of a at Operation of the Druckvariiervorrichtung, in which the reagent vessel is arranged with the therein Reagenzgefäß-insert part, effecting compressive force can be brought into contact with each other so that on the contact, a refractive power to the at least one predetermined breaking point is transferable. Thus, it can be ensured in a simple manner that when a breakage of the at least one predetermined breaking point, the second turret component is already in a position relative to the first turret component, in which a reliable collection of emerging from the first turret component by means of the second turret component is ensured.

For example, the reagent vessel insertion part may include a ballpoint pen mechanism as the elastic spacer component. Thus, a low-cost mechanism for the elastic spacer component can be used.

The above-mentioned advantages are also ensured in a reagent vessel having at least one turret component arranged in the reagent vessel according to the present invention.

In addition, the advantages described are feasible by carrying out the corresponding method for centrifuging a material and the corresponding method for pressure-treating a material.

Brief description of the drawings

Fig. 1a und 1b

eine Draufsicht und einen Querschnitt einer Ausführungsform des Revolverbauteils;

Fig. 2a und 2b

einen Querschnitt und ein Teilquerschnitt einer ersten Ausführungsform eines Reagenzgefäß-Einsetzteils;

Fig. 3

eine schematische Teildarstellung einer zweiten Ausführungsform des Reagenzgefäß-Einsetzteils;

Fig. 4

eine schematische Teildarstellung einer dritten Ausführungsform des Reagenzgefäß-Einsetzteils;

Fig. 5

eine schematische Teildarstellung einer vierten Ausführungsform des Reagenzgefäß-Einsetzteils;

Fig. 6

eine schematische Teildarstellung einer fünften Ausführungsform des Reagenzgefäß-Einsetzteils; und

Fig. 7a bis 7d

schematische Teildarstellungen einer sechsten Ausführungsform des Reagenzgefäß-Einsetzteils.

Further features and advantages of the present invention will be explained below with reference to the figures. Show it:

Fig. 1a and 1b

a plan view and a cross section of an embodiment of the revolver component;

Fig. 2a and 2b

a cross section and a partial cross section of a first embodiment of a Reagenzgefäß insert part;

Fig. 3

a schematic partial view of a second embodiment of the Reagenzgefäß-inserting part;

Fig. 4

a schematic partial view of a third embodiment of the Reagenzgefäß-inserting part;

Fig. 5

a schematic partial view of a fourth embodiment of the Reagenzgefäß-inserting part;

Fig. 6

a schematic partial view of a fifth embodiment of the Reagenzgefäß insert part; and

Fig. 7a to 7d

schematic partial views of a sixth embodiment of the Reagenzgefäß insert part.

Embodiments of the invention

Fig. 1a and 1b show a plan view and a cross section of an embodiment of the revolver component.

This in Fig. 1a and 1b Revolver component 10 shown schematically can be used in a reagent vessel. For example, the revolver component 10 may have a turret outer wall 12, which is designed so that the revolver component 10 can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device. Alternatively or in addition, due to its turret outer wall 12, the turret component 10 may be insertable in an insert part housing of a reagent vessel insert which is adapted for insertion of the reagent vessel insert into a reagent vessel for a centrifuge and / or pressure varying device. The applicability of the revolver component 10 / of the reagent vessel insert into the relevant reagent vessel for a centrifuge and / or a pressure varying device can be interpreted such that the revolver outer wall 12 / an outer wall of the Einsetzteilgehäuses to an inner wall of the reagent vessel corresponds. Preferably, the turret outer wall 12 / the outer wall of the Einsetzteilgehäuses contacted the inner wall of the reagent vessel such that even during operation of the centrifuge and / or the Druckvariiervorrichtung a reliable hold of the turret member 10 / the Reagenzgefäß insertion is ensured in the relevant reagent vessel.

By way of example, the reagent vessel can be understood to mean a (standard) test tube / test tube. Other embodiments include centrifuge tubes, 1.5 ml Eppendorf tubes, 2 ml Eppendorf tubes, 5 ml Eppendorf tubes and microtiter plates, such as e.g. 20 μL microtiter plates (per well). Likewise, the reagent vessel can be a test carrier or a disposable cartridge, which are designed as a lab-on-a-chip system on a plastic-plastic-sized plastic substrate. However, it should be noted that the formability of the reagent vessel is not limited to the examples listed here. In addition, the dimensions of the reagent vessel are predetermined only due to a desired usability of the reagent vessel in the centrifuge and / or in the Druckvariiervorrichtung. However, the feasibility of the technologies according to the invention described below does not prescribe any external form of the reagent vessel. In addition, the reagent vessel can be designed to receive samples in an amount which can be chosen optionally from a range of a few μL up to 1 L.

It should be noted that under the centrifuge and Druckvariiervorrichtung mentioned below, no specific device types are to be understood. Instead, the technology according to the invention can be used by means of any centrifuge, by means of which a (minimum) centrifugal force can be exerted from 20 g. Likewise, the technology according to the invention can be used for any pressure varying device, by means of which a sub and / or overpressure can be applied.

Under the revolver component 10 can be understood in particular a turret for a reagent vessel. The turret component 10 may be designed, for example, such that it can be rotated about an axis of rotation 11 by means of a suitable mechanism which can be arranged on the turret component 10 or separately from the turret component 10. The axis of rotation 11 may, in particular, run centrally through the revolver component 10 and / or be aligned perpendicular to the at least one vessel bottom. In particular, the revolver component 10 / the reagent vessel insertion part can also be designed for cooperation with a ballpoint pen mechanism, or a ballpoint pen mechanism include. The turret member 10 / reagent vial insert may hold a volume less than 5 milliliters. The revolver component 10 can thus be designed in particular such that it can be integrated in a stack of further revolvers and / or reaction chambers. By means of a ballpoint pen mechanism, turrets, reaction chambers and / or cavities (axially stacked one above the other) can be positioned axially as well as azimuthally relative to one another. With regard to a possible execution of the ballpoint pen mechanism is on the DE 2010 003 223 A1 directed.

At least one vessel structure 14, into which at least one liquid and / or pulverized material 16 can be filled, is formed on the revolver component 10. In addition, the revolver component 10 has at least one predetermined breaking point 20 on at least one vessel bottom 18 of the at least one vessel structure 14. By means of a breaking of the at least one predetermined breaking point 20, at least one outlet opening can thus be created, through which the material 16 filled into the at least one vessel structure 14 can flow out / fall out. Thus, the filled into the at least one vessel structure 14 material 16 can be easily removed again. As will be explained in more detail below, by means of forming the at least one predetermined breaking point 20 for a compressive force to be applied for breaking it, a desired / advantageous time for the exit of the material 16 can also be set.

In an advantageous embodiment, at least one predetermined breaking point 20 frames at least one cover element 22 partially so that the respective cover element 22 can be bent out of a plane of the associated predetermined breaking point 20 after breaking the associated predetermined breaking point 20 about a predetermined bending point 24. Thus, it is possible to prevent the material of the turret component 10 which previously covered the outlet opening from falling into a further vessel structure used for collecting the exiting material 16. Instead of the at least one predetermined bending point 24, a hinge component and / or a suspension can also be used.

The at least one predetermined breaking point 20 can in particular be designed in such a way that the at least one predetermined breaking point 20 can be brought about by means of a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged with the revolving component 10 inserted therein, and / or by means of a centrifugal force during operation Druckvariiervorrichtung, in which the reagent vessel is arranged with the turret component 10 inserted therein, frictional effecting frictional effect. Thus, the preferred for breaking the at least one predetermined breaking point 20 time by means of a correspondingly effecting the centrifugal force and / or the pressing force can be set reliably. The at least one predetermined breaking point 20 may for example be designed so that the at least one predetermined breaking point 20 breaks due to a centrifugal force which is exerted by the material 16 stored in the vessel structure 14. This is the case if the centrifugal force exceeds the mechanical stability of the predetermined breaking point 20 above a threshold value, which is typically greater than 20 g. By different interpretations of the threshold value of the respective predetermined breaking point 20 and / or different masses / tightness of the material 16, the at least one predetermined breaking point 20 can be broken at an adjustable / definable time. As will be explained in more detail below, however, the at least one predetermined breaking point 20 is also breakable without a direct breaking of the at least one predetermined breaking point 20 by means of the centrifugal force and / or the pressure force at a desired time.

The turret member 10 may be made in one piece by means of a casting method or an injection molding method despite its advantageous usability. The turret component 10 is thus inexpensive to produce. The internal volume of the turret member 10 / reagent vial insert may be at least partially made of a polymer, e.g. from COP, COC, PC, PA, PU, PP, PET and / or PMMA. Other materials are also suitable for forming the interior volume of the turret member 10 / reagent vial insert.

In addition, at least one channel, at least one cavity and / or at least one reaction chamber may be formed in the revolver component 10 / a reagent vessel insertion part equipped therewith. Process steps and structures, such as, for example, sedimentation structures, channel structures or siphon structures for forwarding and switching at least one liquid contained in the revolver component 10 / the reagent vessel insertion part can be integrated in the inner volume of the revolver component 10 / of the reagent vessel insertion part. In particular, at least one further subunit of the inner volume of the revolver component 10 / of the reagent vessel insertion part can be filled with at least one liquid as a "storage container" which contains at least one chemical reaction and / or with a subsequently filled, to be processed and / or examined material / sample material performs a biochemical / molecular biological process. The at least one "storage container" can eg with chemicals (eg buffers), enzymes, lyphilisates, beads, dyes, antibodies, antigens, receptors, proteins, DNA strands and / or Be filled with RNA strands. The turret member 10 / reagent vial insert may also be equipped with additional components such as valves and / or pumps. In addition, the technology according to the invention can also interact with a multiplicity of conventional actuation, detection and / or control units.

Fig. 2a and 2 B show a cross section and a partial cross section of a first embodiment of a Reagenzgefäß-inserting part.

This in Fig. 2a and 2 B The reagent vessel insertion part 30, shown diagrammatically, has an insertion part housing 32, which is designed such that the reagent vessel insertion part 30 can be inserted in a reagent vessel for a centrifuge and / or for a pressure variation device. The applicability of the reagent vessel insertion part 32 into the relevant reagent vessel for a centrifuge and / or a pressure varying device can be interpreted such that an outer wall 34 of the insertion part housing 32 corresponds to an inner wall of the reagent vessel. Preferably, the outer wall 34 of the Einsetzteilgehäuses 32 contacts the inner wall of the reagent vessel such that even during operation of the centrifuge and / or the Druckvariiervorrichtung a reliable hold of the Reagenzgefäß insertion part 30 is ensured in the relevant reagent vessel. With regard to the reagent vessel into which the reagent vessel insertion part 30 can be inserted, reference is made to the above-enumerated embodiments. However, the reagent vessel cooperating with the reagent vessel insertion part 30 is not limited to these.

In addition, the reagent vessel insertion part 30 includes at least one turret member 10a, 10b, and 10c disposed in the insertion part housing 32. The at least one revolver component 10a, 10b and 10c can be designed so that it can be rotated about the axis of rotation 11. In addition, the at least one turret component 10a, 10b and 10c can also be adjustable along the axis of rotation 11 (lateral). As will be explained in more detail below, a distance between adjacent turret components 10a, 10b and 10c can also be varied in this way. With regard to the further fillability of the at least one turret component 10a, 10b and 10c, reference is made to the above descriptions.

The lateral adjustability of the at least one turret component 10a, 10b and 10c is, for example, by means of a ballpoint pen mechanism 36, which in FIG Fig. 2a only is shown schematically, feasible. (Components of the ballpoint pen mechanism may be formed, for example, as part of the first turret component 10a and / or the second turret component 10b.) Instead of the ballpoint pen mechanism 36, a deformable polymer / elastomer can be used to provide a restoring force, which returns the at least one Revolver components 10a, 10b and 10c in a predetermined starting position / initial position causes. Likewise, a compressible material, such as a polymer, can be used for this purpose. Instead of a compressible material, it is also possible to use a stretchable material which generates a tensile force which, as the restoring force, causes the at least one turret component 10a, 10b and 10c to be returned to a starting position / starting position.

At the in Fig. 2a and 2 B In the embodiment shown, the reagent vessel insertion part 30 has, in addition to a first turret component 10a with the at least one predetermined breaking point 20, at least one second turret component 10b. During operation of a centrifuge and / or a Druckvariiervorrichtung a centrifugal force and / or pressure force can be exerted as actuation force Fa on the at least one predetermined breaking point 20, that this breaks. In this way, a material 16 filled into the at least one vessel structure 14 of the first turret component 10a can be transferred to a further vessel structure 38 of the second turret component 10b. Preferably, therefore, the second turret member 10b is aligned with respect to the first turret member 10a in the direction of the actuator force Fa. In addition, by reducing the distance between the two turret members 10a and 10b due to the actuator force Fa counteracting the ballpoint pen mechanism 36 or similar component, leakage-free transfer of the liquid 16 on the first turret member can be achieved 10a are secured in the second turret component 10c. The reaction vessel insertion part 30 is therefore particularly suitable for swing-bucket rotors. (The actuation force Fa may also be used to actuate the ballpoint pen mechanism 36 or a similar component.)

Fig. 3 shows a schematic partial view of a second embodiment of the Reagenzgefäß insertion part.

This in Fig. 3 partially re-introduced reaction vessel insertion part 30 has at least one turret component 10a with at least one predetermined breaking point 20, wherein the turret component 10a and / or in the at least one vessel structure 14 has at least one holding device 40, by means of which at least one mass portion 42 with a retaining force on and / or in the at least one holding device 40 is durable. The at least one holding device 40 is also arranged to the at least one predetermined breaking point 20, that after releasing the restraining force, the at least one released mass portion 42 on the at least one predetermined breaking point 20 and / or at least one of the at least one predetermined breaking point 20 at least partially framed bottom surface For example, the lid member 22 falls. The at least one mass part 42 thus acts as an actuation element for breaking the at least one predetermined breaking point 20. The cancellation of the retaining force can be effected in particular by means of a centrifugal force and / or a compressive force during operation of a centrifuge and / or a pressure varying device. Thus, the time for canceling the restraining force which triggers an acceleration of the mass part 42 along a movement path 44 oriented in the direction of the actuation force Fa can also be advantageously set.

At the in Fig. 3 schematically represented embodiment, the at least one predetermined breaking point 20 may be designed so that it does not break even at a centrifugal acceleration of about 10000g, or at a corresponding compressive force, and despite the mass of filled in the at least one vessel structure 14 material 16 and is stable , By releasing the restraint force of the holding device 40, the mass portion 42 can be released and accelerated by means of the actuation force Fa so that it breaks when breaking on or at the predetermined breaking point 20. As a retaining device 40, for example, a magnet, in particular a permanent magnet, are used, which extracts the at least partially formed of a magnetically attractable material mass portion 42. Likewise, the holding device 40 may be a mechanically biased holding device 40, in which the mass portion 42 is inserted so that it dissolves from a force equal to the retaining force of the mechanically biased holding device 40. It should also be noted that the holding device 40 may be an actively controllable actuator which can be formed, for example, by means of an actively activatable magnet or a coil. The advantageous position of the at least one holding device 40 can be ensured by attaching it to a cover element 46 / cover. However, the examples of forming and arranging the retainer 40 and mass portion 42 described herein are to be interpreted as exemplary only.

Fig. 4 shows a schematic partial view of a third embodiment of the Reagenzgefäß-inserting part.

This in Fig. 4 Partially schematically reproduced reagent vessel insertion part 30 comprises at least the first turret component 10a with the at least one predetermined breaking point 20 and the second turret component 10b. The first turret component 10a and the second turret component 10b are arranged relative to one another by means of an elastic spacer component 48 such that the first turret component 10a and the second turret component 10b use an actuation force Fa, such as during operation of a centrifuge, in the rotor device of the reagent vessel arranged therein inserted reagent vessel insertion part 30, effecting centrifugal force and / or by means of a in a operation of a Druckvariiervorrichtung, in which the reagent vessel is arranged with the Reagenzgefäß-inserting member 30, cause compressive force, so that they can be brought into contact with each other over the contact a refractive power to the at least one predetermined breaking point 20 is transferable. As the elastic spacer component 48, the reagent vessel insertion part 30 may include, for example, a ballpoint pen mechanism and / or the corresponding components set forth above.

By means of a specification of the actuation force Fa, from which the retention force of the elastic spacer component 48 is canceled so that the contact for breaking the predetermined breaking point 20 is present, the time of breaking of the at least one predetermined breaking point 20 can be adjusted. Thus, the embodiment described here ensures the above-mentioned advantages.

It is particularly noted that in the embodiment of the Fig. 4 a break of at least one predetermined breaking point 20 only takes place when the contact between the two turret components 10a and 10b is present. Thus, it can be reliably ensured that the liquid emerging from the at least one vessel structure 14 of the first turret component 10a can be reliably collected by means of the second turret component 10b.

In the embodiment of the Fig. 4 is at one of a predetermined breaking point 20 partially framed floor area, such as the lid member 22, a highlight 50 is formed. The highlighting 50 preferably extends along the direction of the actuation force Fa, for example radially outward and / or inward Direction to the second turret component 10b. The highlighting 50 may be formed, for example, as an elevation, as a bridge and / or as a pin. A reduction in the (lateral) distance between the two turret components 10a and 10b by means of the actuation force Fa results in contact between the highlight 50 and the second turret component 10b. The highlight 50 may contact the second turret member 10b, for example, from a centrifugal acceleration of 20 g. A further increase in the actuation force Fa, for example due to an increase in the centrifugal acceleration, can lead to a breakage of the respective predetermined breaking point 20. In particular, after breaking the associated predetermined breaking point 20, the cover element 22 can be pressed into the associated vessel structure 14 due to the contact between the highlighting 50 and the second turret component 10b. In this way, the material 16 introduced into the associated vessel structure 14 can be quickly released.

The highlighting 50 preferably contacts the second turret component 10b at a contact surface 52 which lies within the further vessel structure 38 of the second turret component 10b. For example, the contact surface 52 can lie on a step 54 formed in the further vessel structure 38 or a corresponding support structure / emphasis. Instead of stage 54, e.g. Also, a web or a pin in the further vessel structure 38 may be formed. In this way, it can be ensured that the breaking of the assigned predetermined breaking point 20 does not take place until the outlet opening exposed in this way is advantageously positioned relative to the further vessel structure 38.

Fig. 5 shows a schematic partial view of a fourth embodiment of the Reagenzgefäß-inserting part.

At the in Fig. 5 partially shown reagent vessel insertion part 30, the emphasis 50 is attached to a mounting surface 56 of the second turret component 10b. The attachment surface 56 is preferably located within a further vessel structure 38 of the second turret component 10b, for example on a step 54 or on a corresponding support structure / emphasis. (Under the support structure, a web or a pin can be understood.) Thus, it is ensured in this case that the desired breaking of the associated predetermined breaking point 20 and / or inward bending of the contacted cover member 22 only in an advantageous position of the vessel structures 14 and 38 to each other he follows.

Fig. 6 shows a schematic partial view of a fifth embodiment of the Reagenzgefäß-inserting part.

At the in Fig. 6 In some embodiments, the geometry of a contact side 58 of the first turret component 10a aligned with the second turret component 10b is designed such that the contact side 58 can dip into the at least one further vessel structure 38 of the second turret component 10b. In addition, the emphasis 50 attached to the first turret component 10a is designed so short that the breaking of the assigned predetermined breaking point 20 takes place only after a partial immersion of the first turret component 10a into the second turret component 10b. Thus, the material 16 filled into the at least one vessel structure 14 can be transferred free of leakage from the at least one vessel structure 14 of the first revolver component 10a into at least one further vessel structure 38 of the second revolver component 10b. It should be noted that this leakage-free transfer is ensured even if the actuation force Fa is inclined to the axis of rotation 11 and / or a longitudinal axis of the Reagenzgefäß-Einsetzteils aligned. This is the case, for example, during processing in the fixed-angle rotor.

The advantageous plug-socket principle of the embodiment of Fig. 6 is formed by the surfaces of the turret components 10a and 10b are not only designed planar and parallel to each other, but provided with elevations and depressions, which are partially offset, complementary and / or overlapping each other.

Fig. 7a to 7d show schematic partial views of a sixth embodiment of the Reagenzgefäß insert part.

In the Fig. 7a to 7d schematically partially reproduced embodiment has as a supplement to the previous embodiment, a second turret component 10b, at least two further vessel structures 38-1 and 38-2 each have a protruding from a bottom surface 62 of the other vessel structure 38 web element 60-1 and 60-2. A first web element 60-1 of a first vessel structure 38-1 of the second turret component 10b is arranged in a first distance a1 directed radially away from the rotation axis 11 to a side inner wall 64-1 of the first vessel structure 38-1 which extends radially from the axis of rotation 11 directed away second distance a2 in which a second web element 60-2 of a second vessel structure 38-2 of the second turret component 10b is arranged to a side inner wall 64-2 of the second vessel structure 38-2. Under the side inner walls 64-1 and 64-2 side wall surfaces can be understood on an outer wall protruding from the bottom surface 62, wherein the outer wall can be aligned in particular (almost) parallel to the axis of rotation 11. (If the second turret component 10b also comprises at least one third vessel structure, its web element can be arranged in a third distance directed radially away from the rotation axis 11 to a side inner wall of the third vessel structure which is not equal to the distances a1 and a2 may thus be equal to the number of vessel structures of the second turret component 10b.)

Preferably, each of the at least two vessel structures 38-1 and 38-2 of the second turret component 10b is assigned a respective vascular structure 14-1 and 14-2 of the first turret component 10a. The respective vessel structure 14-1 and 14-2 of the first turret component 10a has an emphasis 50-1 and 50-2, which in a radially away from the rotation axis 11 directed away distance a1 or a2 to an adjacent to the contact side 58 edge 66 of a Side outer wall 68 of the first turret member 10a on the contact side 58 is arranged. A first emphasis 50-1 of a first vessel structure 14-1 of the first turret component 10a, which is assigned to the first vessel structure 38-1 of the second turret component 10b, is thus in the radial direction away from the rotation axis 11 first distance a1 to the edge 66 of Side outer wall 68 of the first turret member 10a disposed on the contact side 58. Correspondingly, a second emphasis 50-2 of a second vessel structure 14-2 of the first turret component 10a, which is assigned to the second vessel structure 38-1 of the second turret component 10b, in the second distance a2 directed radially away from the rotation axis 11 to the edge 66 of FIG Side outer wall 68 of the first turret member 10a disposed on the contact side 58. In this way, a switching logic can be realized by means of which a first material 16-1 filled into the first vessel structure 14-1 of the first turret component 10a and a different second material 16-2 filled into the second vessel structure 14-2 of the first turret component 10a are selectively / purposefully in certain vascular structures 38-1 and 38-2 of the second turret component 10b are transferable.

Fig. 7a shows a rotational position of the two turret components 10a and 10b to each other, in which the vessel structures 14-1 and 14-2 of the first turret member 10a to a Angle of rotation α not equal to zero with respect to their associated vessel structures 38-1 and 38-2 of the second turret component 10b are rotated. (The non-zero rotational angle α is in Fig. 7b For the sake of clarity, the distance is shown as a distance.) If the restraining force of the elastic spacer component at this non-zero rotational angle is canceled by the actuator force Fa, the first turret member 10a partially plunges into the second turret member 10b, however, each of the vessel structures 14-1 and 14-2 of the first turret component 10a immersed in a unassigned vessel structure 38-1 and 38-2 of the second turret component 10b (see Fig. 7b ). The first vessel structure 14-1 of the first turret component 10a dips, for example, into the second vessel structure 38-1 of the second turret component 10b. However, due to the different distances a1 and a2, the first highlight 50-1 of the first vessel structure 14-1 of the first turret member 10a along its dip path 69-1 does not strike the second support structure 60-2 of the second vial structure 38-2 of the second turret member 10b. (Similarly, the second highlight 50-2 of the second vessel structure 14-2 of the first turret member 10a does not contact the first bearing structure 60-1 of the first vessel structure 38-1 of the second turret member 10b along its dip path 69-2.) The difference between the distances a1 and a2 is thus selected to be sufficiently large to allow contact between the first prominence 50-1 of the first vessel structure 14-1 of the first revolving member 10a and the second abutment structure 60-2 of the second vessel structure 38-1 of the second revolving member 10b, despite the immersion of the prevent first vessel structure 14-1 of the first turret member 10a in the second vessel structure 38-1 of the second turret member 10b. In addition, the first turret component 10a on the side outer wall 68 on a diameter extension, which is formed for example as an external step 70. The space defined by the side inner walls 64-1 and 64-2 of the second turret component 10b has a maximum extent perpendicular to the axis of rotation which is smaller than the maximum diameter of the side outer wall 68 of the first turret component 10a. Thus, a holding structure is formed between the two turret components, which even in a further pressing against each other of the two turret components 10a and 10b a deeper immersion of the first turret member 10a in the second turret member 10b prevented.

The second turret component 10b thus exerts no force on the predetermined breaking points 20 of the first turret component 10a, despite the immersion of the first vessel structure 14-1 of the first turret component 10a into the second vessel structure 38-2 of the second turret component 10b. The two turret parts 10a and 10b are then by means of a Force Fk of a (not outlined) ballpoint pen mechanism pulled apart again. Subsequent rotation 72 of the first turret member 10a relative to the second turret member 10b places the two turret members 10a and 10b in a position in which the vascular structures 14-1 and 14-2 of the first turret member 10a are associated with their associated vascular structures 38-1 and 38-2 of the second turret component 10b are aligned (see Fig. 7c ). A renewed tightening of the two turret components 10a and 10b (eg by means of the actuation force Fa) thus leads to immersion of the first vessel structure 14-1 of the first turret component 10a into the first vessel structure 38-1 of the second turret component 10b and to immersion of the second vessel structure 14 -2 of the first turret component 10a into the second vessel structure 38-2 of the second turret component 10b. In this case, the emphasis 50-1 and 50-2 respectively contact their associated support structures 60-1 and 60-2, so that the predetermined breaking points 20 are broken and material outlet openings in the vessel structures 14-1 and 14-2 of the first turret component 10a are opened. The various materials 16-1 and 16-2 can thus be filled in a targeted manner into their assigned vessel structures 38-1 and 38-2 of the second turret component 10b.

In an advantageous development, a vessel structure 38-1 and 38-2 of the second turret component 10b can also have a plurality of support structures 60-1 and 60-2 at different positions. In this way, the vessel structure 38-1 and 38-2 of the second turret member 10b may be coupled to a plurality of vessel structures 14-1 and 14-2 of the first turret member 10a. The further vessel structure 38-1 and 38-2 of the second turret component 10b can thus be used advantageously as a mixing and / or incubation chamber. In addition, a vessel structure 14-1 and 14-2 of the first turret member 10a may have a plurality of highlights 50-1 and 50-2 at different positions.

The embodiments described in the upper paragraphs can also be combined with each other differently.

In addition, the statements made in the above paragraphs regarding a reagent vessel insertion part according to the technology according to the invention also apply to a reagent vessel for a centrifuge and / or a pressure varying device, which is designed in accordance with the described reagent vessel insertion parts. The advantageous reagent vessel has an outer wall, which is formed so that the reagent vessel in a centrifuge and / or in a Druckvariiervorrichtung can be used. In particular, the reagent vessel is designed so that a reliable hold of the reagent vessel is ensured in the operated centrifuge and / or in the operated Druckvariiervorrichtung. A reagent vessel for a centrifuge and / or a pressure variegating device can thus be understood to mean a reagent vessel which, due to its (external) shape, lends itself well to operation of the centrifuge with a comparatively high rotational speed and / or application of a superordinate to the atmospheric pressure - And / or negative pressure by means of Druckvariiervorrichtung. The advantageous reagent vessel may have vessel structures, such as channels, reaction chambers, storage chambers and / or active components, such as valves and / or pumps. In addition, the reaction vessel may comprise actuation, detection and control units. Thus chemical reactions and / or biochemical / molecular biological processes can be fully automated in the reagent vessel.

The advantageous reagent vessel has at least one turret component arranged in the reagent vessel with at least one predetermined breaking point. In addition, in addition to a first turret component with the at least one predetermined breaking point, the reagent vessel may also comprise at least one second turret component, wherein the first turret component and the second turret component are arranged relative to each other by means of a resilient spacer component such that the first turret component and the second turret component by means of a Operation of a centrifuge, in the rotor device, the reagent vessel is arranged, effecting centrifugal force and / or by means of a force in an operation of a Druckvariiervorrichtung in which the reagent vessel, be effected compressive force can be brought into contact with each other so that a contact force on the at least one predetermined breaking point is transferable. For example, the reagent vessel may include a ballpoint pen mechanism as the resilient spacer component. The other embodiments described above are also applicable to the advantageous reagent container.

The advantages enumerated in the above paragraphs are also ensured in carrying out the method of centrifuging a material and the method of pressure treating a material.

Claims (15)

Revolver component (10, 10a) for a reagent vessel,
wherein at least one vessel structure (14, 14-1, 14-2), into which at least one liquid and / or pulverized material (16, 16-1, 16-2) can be introduced, is formed on the revolver component (10, 10a);
characterized in that
the revolver component (10, 10a) has at least one predetermined breaking point (20) on at least one vessel bottom (18) of the at least one vessel structure (14, 14-1, 14-2). A turret component (10, 10a) according to claim 1, wherein the turret member (10, 10a) is integrally formed by a casting method or an injection molding method. Revolver component (10, 10a) according to claim 1 or 2, wherein the at least one predetermined breaking point (20) at least one cover element (22) partially framed so that the respective cover element (22) after breaking the associated predetermined breaking point (20) about a predetermined bending point ( 24) out of a plane of the associated predetermined breaking point (20) can be bent out. A turret component (10, 10a) according to any one of the preceding claims, wherein the turret member (10, 10a) has a turret outer wall (12) which is configured such that the turret member (10, 10a) is mounted in a reagent vessel for a centrifuge and / or a Druckvariiervorrichtung is used. A turret member (10, 10a) according to any one of the preceding claims, wherein the turret member (10, 10a) is insertable in an insert part housing (32) of a reagent vial inserting part (30) which is formed so that the reagent vial inserting part (30) in a reagent vessel for a centrifuge and / or for a Druckvariiervorrichtung is used. Revolver component (10, 10a) according to claim 4 or 5, wherein the at least one predetermined breaking point (20) is formed such that the at least one predetermined breaking point (20) by means of a centrifuge in operation, in the rotor device, the reagent vessel with the turret component inserted therein (10, 10a) is arranged, effecting centrifugal force and / or by means of a in a operation of the Druckvariiervorrichtung, in which the reagent vessel is arranged with the turret component (10, 10a) is arranged, frictional compressive force. Revolver component (10, 10a) according to one of the preceding claims, wherein the revolver component (10, 10a) on and / or in the at least one vessel structure (14, 14-1, 14-2) at least one holding device (40), by means of which at least one mass portion (42) with a retaining force on and / or in the at least one holding device (40) is durable, and wherein the at least one holding device (40) is arranged to the at least one predetermined breaking point (20) that after lifting the Retaining force that at least one released mass part (42) on the at least one predetermined breaking point (20) and / or at least one of the at least one predetermined breaking point (20) at least partially framed bottom surface (22) falls. Reagent vessel insert (30) with: an insert part housing (32) which is designed such that the reagent vessel insertion part (30) can be inserted in a reagent vessel for a centrifuge and / or for a pressure-varying device; and at least one turret component (10, 10a) arranged in the insertion part housing (32) according to one of the preceding claims. Reagent vessel insertion part (30) according to claim 8, wherein the reagent vessel insertion part (30) in addition to a first turret component (10a) with the at least one predetermined breaking point (20) still at least a second turret component (10b), and wherein the first turret component ( 10 a) and the second turret component (10 b) by means of a elastic spacer component (36, 48) are arranged to each other, that the first turret component (10a) and the second turret component (10b) by means of a centrifuge in operation, in the rotor device, the reagent vessel with the reagent vessel inserting part (30) inserted therein is, cause centrifugal force and / or by means of a in operation of the Druckvariiervorrichtung, in which the reagent vessel is arranged with the Reagenzgefäß insertion part (30) arranged, compressive force can be brought into contact with each other so that a contact force on the at least one predetermined breaking point (20) is transferable. The reagent container insertion part (30) according to claim 9, wherein the reagent vessel insertion part (30) comprises a ball point pen mechanism (36) as the elastic spacer component (36). Reagent vessel for a centrifuge and / or for a pressure-varying device with: at least one turret component (10, 10a) arranged in the reagent vessel according to one of claims 1 to 7. Reagent vessel according to claim 11, wherein the reagent vessel in addition to a first turret component (10a) with the at least one predetermined breaking point (20) still at least a second turret component (10b), and wherein the first turret component (10a) and the second turret component (10b) means a resilient spacer component (36, 48) are arranged relative to one another such that the first turret component (10a) and the second turret component (10b) are caused by a centrifugal force which can be effected during operation of the centrifuge, in whose rotor device the reagent vessel is arranged, and / or by means of a centrifugal force in an operation of the Druckvariiervorrichtung, in which the reagent vessel is arranged, effecting compressive force can be brought into contact with each other so that on the contact a refractive power to the at least one predetermined breaking point (20) is transferable. The reagent vessel of claim 12, wherein the reagent vessel comprises a ballpoint pen mechanism (36) as the resilient spacer component (36). Method for centrifuging a material (16, 16-1, 16-2) with the steps: Filling the material to be centrifuged (16, 16-1, 16-2) into a reagent vessel with a reagent vessel inserting part (30) according to any one of claims 8 to 10 inserted therein and / or into a reagent vessel according to one of claims 11 to 13; and At least operating the centrifuge at a rotational speed which causes a centrifugal force to break the at least one predetermined breaking point (20). Method of pressure treating a material (16, 16-1, 16-2) comprising the steps of: Filling the material to be treated (16, 16-1, 16-2) into a reagent vessel with a reagent vessel inserting part (30) according to any one of claims 8 to 10 inserted therein and / or into a reagent vessel according to one of claims 11 to 13; and At least once applying a negative or positive pressure, which causes a compressive force to break the at least one predetermined breaking point (20).

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