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EP1855804A1 - Tip for sampling pipette and pipette thus equipped - Google Patents

Tip for sampling pipette and pipette thus equipped

Info

Publication number
EP1855804A1
EP1855804A1 EP06709248A EP06709248A EP1855804A1 EP 1855804 A1 EP1855804 A1 EP 1855804A1 EP 06709248 A EP06709248 A EP 06709248A EP 06709248 A EP06709248 A EP 06709248A EP 1855804 A1 EP1855804 A1 EP 1855804A1
Authority
EP
European Patent Office
Prior art keywords
pipette
cone
tip
end part
sampling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP06709248A
Other languages
German (de)
French (fr)
Inventor
Yves May
Bernard Roussel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gilson SAS
Original Assignee
Gilson SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilson SAS filed Critical Gilson SAS
Publication of EP1855804A1 publication Critical patent/EP1855804A1/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing

Definitions

  • the invention relates to the field of laboratory pipettes, intended for the calibrated sampling and introduction of liquids into containers.
  • Laboratory pipettes have one or more tips, generally removable, of substantially frustoconical exterior shape. Each of these tips is equipped with a removable and disposable plastic sampling cone. Conventionally, each of these cones is fitted onto the corresponding end piece and is held on the end piece by friction.
  • the inner surface of the sampling cone has a substantially conical longitudinal section, the dimensions of which, in the zone where said friction with the tip must be achieved, must be such as to allow the cone to be fitted onto the tip and its fixing. with suitable holding force and tightness. Ejection of the cone is preferably carried out using a suitable device integrated into the pipette, such as, for example, that described in document FR-A-2 807 342.
  • the force to be exerted for the ejection of the cone is random, and can be very high if the cone has been fitted onto the end piece with a significant force and depth of insertion.
  • the aim of the invention is to provide a pipette tip that best solves the problems just mentioned.
  • the invention relates to a sampling pipette tip, the substantially frustoconical or cylindrical end part of which is intended to receive by fitting a sampling cone, characterized in that said end part comprises at least two annular shoulders at the ends. levels at which the diameter of said end part decreases towards the lower end of the end piece, said shoulders providing at their levels a reduction in the diameter of said end part from 0.2 to 2mm, preferably 0.3 to 1.8mm .
  • Said shoulders can be rounded.
  • Said shoulders can then extend over a length of 0.2 to 5mm, preferably 0.5 to 3mm.
  • the distance between the shoulders can be 2 to 8mm, preferably 3.5 to 6mm.
  • Tip can be adapted for use on a multichannel pipette
  • the subject of the invention is also a sampling pipette, characterized in that it comprises at least one tip of the above type.
  • the invention can be a multichannel pipette.
  • the invention is based on a new pipette tip configuration.
  • the outer surface of its lower end portion comprises at least two annular stepped shoulders of dimensions chosen from a precise range, constituting preferred contact zones with the sampling cone.
  • the lower shoulder seals the cone / tip contact.
  • the upper shoulder essentially serves as an aid in centering the cone on the end piece and as a stop.
  • FIG. 1 which shows, seen in longitudinal section, the end of an exemplary pipette tip according to the invention, equipped with its sampling cone;
  • the tip example 1 according to the invention shown in Figure 1 has, as is known, in its lower end part 2, a generally frustoconical outer shape so as to allow the fitting on said end part 2 of a sampling cone 3, the interior space 4 of which is shaped with a corresponding frustoconical longitudinal section in the zone where contact with the tip 1 is to take place.
  • This contact must have the best possible seal compatible with easy ejection of the cone 3 when the latter must be replaced.
  • the cone 3 may have a completely usual configuration and dimensions, for example a strictly cylindrical or frustoconical inner surface, without hollow or landforms.
  • constituent material which may be of any nature known to be suitable for the manufacture of pipette tips.
  • the tip 1 according to the invention can be designed to equip a pre-existing pipette by simply replacing a removable tip of conventional configuration. It can also constitute a non-removable end of a pipette which, moreover, would be of the usual design.
  • the end part 2 of the end piece 1 has two annular shoulders: an upper shoulder 5 and a lower shoulder 6. They each provide at their level a relatively sudden change in the outer diameter of the end piece 1, namely a reduction in this diameter in the direction of the lower end 8 of the nozzle 1. They successively come into contact with the inner wall 7 of the cone 3 when fitting the cone 3 on the nozzle 1, and allow ensure the function of maintaining the cone 3 on the end piece 1 with better efficiency than when, conventionally, the end part 2 of the end piece 1 has a diameter which constantly decreases progressively towards its lower end 8.
  • shoulders 5, 6 each correspond to a reduction in the diameter of the end piece 1 which is from 0.2 to 2mm, preferably from 0.3 to 1.8mm.
  • these shoulders 5, 6 do not have a sharp angle but are rounded to facilitate the fitting of the cone 3.
  • the reduction in the diameter of the end piece 1 corresponding to a shoulder 5, 6 is carried out over a length from 0.2 to 5mm, preferably from 0.5 to 3mm.
  • the main function of the upper shoulder 5 is to constitute both a centering member of the cone 3 during fitting and a progressive stop.
  • the lower shoulder 6, for its part, essentially ensures the tightness of the cone 3 / end piece 1 connection, and it is at its level that the very localized deformation of the cone 3 caused by the fitting is strongest.
  • the distance between the two shoulders 5, 6 is typically of the order of 2 to 8mm, preferably 3.5 to 6mm. The precise choices of these dimensions depend essentially on the characteristics of the cones which will be used. As shown, the cone 3 can, conventionally, contain a filter 9; but this characteristic, independent of the invention, is of course not at all compulsory.
  • the invention makes it possible to make the geometry of the cone 3 / tip 1 contact more reliable, the quality of which thus becomes much less dependent than in the prior art on the force exerted by the user when fitting the cone 3. Due to the fact , in particular, the existence of the stop constituted by the upper shoulder 5, a high fitting force does not lead to a depression of the end part 2 of the end piece 1 in the cone 3 substantially greater than that provided by less fitting effort.
  • this contact pressure is very variable from one tip to another and that the highest pressures are reached, as is generally observed, for the tips located in the lateral zones of the pipette.
  • Curve 14 shows this same contact pressure, measured at the level of the sealing zones of each of the 12 tips of a multichannel pipette whose tips are shaped according to the invention and whose cones are identical to those of the previous conventional pipette. . It can be seen that: - this contact pressure is situated at values which are appreciably higher than for the conventional pipette; this is due, as has been said, to the smaller surface area of the sealing zone;
  • the pipette tip according to the invention can be used for single-channel or multi-channel pipettes.
  • the typical characteristics of the invention can be used indifferently in the two cases.
  • the other characteristics of the tip are the usual characteristics making them suitable for use on either of these types of pipettes.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

The invention relates to a tip for a sampling pipette, the end part of which (2) essentially takes the form of a truncated cone or a cylinder in which a sampling cone (3) is inserted. The invention is characterised in that the aforementioned end part comprises at least two annular flanges (5, 6), whereat the diameter of said end part (2) diminishes in the direction of the lower end (8) of the tip. The invention is further characterised in that the flanges provide a reduction in the diameter of the end part (2) of between 0.2 and 2 mm and, preferably, between 0.3 and 1.8 mm. The invention also relates to a sampling pipette which is equipped with said tip.

Description

Embout pour pipette de prélèvement, et pipette ainsi équipée. Tip for sampling pipette, and pipette thus equipped.
L'invention concerne le domaine des pipettes de laboratoire, destinées au prélèvement et à l'introduction calibrées de liquides dans des récipients.The invention relates to the field of laboratory pipettes, intended for the calibrated sampling and introduction of liquids into containers.
Les pipettes de laboratoire comportent un ou de multiples embouts, généralement amovibles, de forme extérieure sensiblement tronconique. Chacun de ces embouts est équipé d'un cône de prélèvement en matière plastique amovible et jetable. Classiquement, chacun de ces cônes est emmanché sur l'embout correspondant et est maintenu sur l'embout par friction. La surface intérieure du cône de prélèvement présente une section longitudinale sensiblement conique dont les dimensions, dans la zone où ladite friction avec l'embout doit être réalisée, doivent être telles qu'elles autorisent l'emmanchement du cône sur l'embout et sa fixation avec une force de maintien et une étanchéité convenables. L'éjection du cône est, de préférence, réalisée à l'aide d'un dispositif approprié intégré à la pipette, tel que, par exemple, celui décrit dans le document FR-A-2 807 342.Laboratory pipettes have one or more tips, generally removable, of substantially frustoconical exterior shape. Each of these tips is equipped with a removable and disposable plastic sampling cone. Conventionally, each of these cones is fitted onto the corresponding end piece and is held on the end piece by friction. The inner surface of the sampling cone has a substantially conical longitudinal section, the dimensions of which, in the zone where said friction with the tip must be achieved, must be such as to allow the cone to be fitted onto the tip and its fixing. with suitable holding force and tightness. Ejection of the cone is preferably carried out using a suitable device integrated into the pipette, such as, for example, that described in document FR-A-2 807 342.
Les embouts de pipettes existants et les cônes de prélèvement correspondants tels qu'on vient de les décrire ne permettent cependant pas d'obtenir des propriétés d'usage complètement satisfaisantes. En effet :The existing pipette tips and the corresponding sampling cones as just described do not, however, make it possible to obtain completely satisfactory properties of use. Indeed :
- l'effort nécessaire à l'emmanchement des cônes est aléatoire, et peut être très important ; de plus, rien n'est prévu pour limiter cet effort ;- the force required for fitting the cones is random, and can be very high; moreover, nothing is planned to limit this effort;
- les pressions de contact au niveau de la zone d'étanchéité sont faibles ; il s'ensuit que la qualité de l'étanchéité devient aléatoire et non reproductible ;- the contact pressures at the sealing zone are low; it follows that the quality of the seal becomes random and not reproducible;
- la hauteur et la position de la zone d'étanchéité ne sont pas maîtrisées de façon suffisamment strictes ; cela représente un inconvénient notamment pour les pipettes multicanaux, qui comportent plusieurs embouts alignés sur lesquels on adapte simultanément un nombre équivalent de cônes disposés dans un rack ou reliés l'un à l'autre pour former une barrette ; dans ce cas, il n'est pas garanti que le positionnement de tous les cônes soit correct, et, donc, qu'ils procurent tous une étanchéité satisfaisante du contact avec l'embout correspondant ;- the height and position of the sealing zone are not controlled in a sufficiently strict manner; this represents a drawback in particular for multichannel pipettes, which comprise several aligned tips on which an equivalent number of cones arranged in a rack or connected to one another to form a strip is simultaneously fitted; in this case, it is not guaranteed that the positioning of all the cones is correct, and, therefore, that they all provide a satisfactory seal on contact with the corresponding end piece;
- l'effort à exercer pour l'éjection du cône est aléatoire, et peut être très élevé si le cône a été emmanché sur l'embout avec une force et une profondeur d'insertion importantes.- The force to be exerted for the ejection of the cone is random, and can be very high if the cone has been fitted onto the end piece with a significant force and depth of insertion.
Il a été proposé dans le document WO-A-2003/002980 d'améliorer l'étanchéité de la liaison cône-embout en prévoyant un bourrelet annulaire sur l'embout. Ce bourrelet procure un épaississement localisé de l'embout et constitue souvent une butée contre laquelle vient reposer une portée ménagée sur la paroi interne du cône. Cependant, ce bourrelet tend à rendre l'insertion du cône plus difficile et nécessite des cônes présentant une conformation et une déformabilité inhabituelles dans la zone de contact entre le cône et le bourrelet.It has been proposed in document WO-A-2003/002980 to improve the tightness of the cone-nozzle connection by providing an annular bead on the nozzle. This bead provides localized thickening of the end piece and often constitutes a stop against which a bearing provided on the internal wall of the cone rests. However, this bead tends to make insertion of the cone more difficult and requires cones exhibiting unusual conformation and deformability in the zone of contact between the cone and the bead.
Le but de l'invention est de proposer un embout de pipette résolvant au mieux les problèmes que l'on vient de citer. A cet effet, l'invention a pour objet un embout de pipette de prélèvement, dont la partie terminale sensiblement tronconique ou cylindrique est destinée à recevoir par emmanchement un cône de prélèvement, caractérisé en ce que ladite partie terminale comporte au moins deux épaulements annulaires aux niveaux desquels le diamètre de ladite partie terminale diminue en direction de l'extrémité inférieure de l'embout, lesdits épaulements procurant à leurs niveaux une diminution du diamètre de ladite partie terminale de 0,2 à 2mm, préférentiellement 0,3 à 1 ,8mm.The aim of the invention is to provide a pipette tip that best solves the problems just mentioned. To this end, the invention relates to a sampling pipette tip, the substantially frustoconical or cylindrical end part of which is intended to receive by fitting a sampling cone, characterized in that said end part comprises at least two annular shoulders at the ends. levels at which the diameter of said end part decreases towards the lower end of the end piece, said shoulders providing at their levels a reduction in the diameter of said end part from 0.2 to 2mm, preferably 0.3 to 1.8mm .
Lesdits épaulements peuvent être arrondis.Said shoulders can be rounded.
Lesdits épaulements peuvent alors s'étendre sur une longueur de 0,2 à 5mm, préférentiellement de 0,5 à 3mm.Said shoulders can then extend over a length of 0.2 to 5mm, preferably 0.5 to 3mm.
La distance entre les épaulements peut être de 2 à 8mm, de préférence 3,5 à 6mm.The distance between the shoulders can be 2 to 8mm, preferably 3.5 to 6mm.
L'embout peut être adapté à une utilisation sur une pipette multicanauxTip can be adapted for use on a multichannel pipette
L'invention a également pour objet une pipette de prélèvement, caractérisée en ce qu'elle comporte au moins un embout du type précédent.The subject of the invention is also a sampling pipette, characterized in that it comprises at least one tip of the above type.
Il peut s'agir d'une pipette multicanaux. Comme on l'aura compris, l'invention repose sur une nouvelle configuration d'embout de pipette. La surface extérieure de sa partie terminale inférieure comporte au moins deux épaulements annulaires étages de dimensions choisies dans une plage précise, constituant des zones de contact privilégiées avec le cône de prélèvement.It can be a multichannel pipette. As will be understood, the invention is based on a new pipette tip configuration. The outer surface of its lower end portion comprises at least two annular stepped shoulders of dimensions chosen from a precise range, constituting preferred contact zones with the sampling cone.
L'épaulement inférieur procure l'étanchéité du contact cône/embout. L'épaulement supérieur sert essentiellement d'aide au centrage du cône sur l'embout et de butée.The lower shoulder seals the cone / tip contact. The upper shoulder essentially serves as an aid in centering the cone on the end piece and as a stop.
L'invention sera mieux comprise à la lecture de la description qui suit, donnée en référence aux figures annexées suivantes :The invention will be better understood on reading the following description, given with reference to the following appended figures:
- la figure 1 qui montre, vue en coupe longitudinale, l'extrémité d'un exemple d'embout de pipette selon l'invention, équipé de son cône de prélèvement ;- Figure 1 which shows, seen in longitudinal section, the end of an exemplary pipette tip according to the invention, equipped with its sampling cone;
- la figure 2 qui montre de la même façon un détail dudit embout équipé de son cône ;- Figure 2 which shows in the same way a detail of said tip equipped with its cone;
- la figure 3 qui montre schématiquement la courbe « effort d'éjection = f (effort d'emmanchement) » d'un cône sur un embout de pipette classique et sur un embout de pipette selon l'invention ;FIG. 3 which schematically shows the curve “ejection force = f (fitting force)” of a cone on a conventional pipette tip and on a pipette tip according to the invention;
- la figure 4 qui montre schématiquement la pression de contact entre les différents cônes et les différents embouts dans le cas d'une pipette multicanaux classique et d'une pipette multicanaux équipée d'embouts.- Figure 4 which schematically shows the contact pressure between the different cones and the different tips in the case of a conventional multichannel pipette and a multichannel pipette equipped with tips.
L'exemple d'embout 1 selon l'invention représenté sur la figure 1 présente, comme il est connu, dans sa partie terminale inférieure 2, une forme extérieure générale tronconique de façon à permettre l'emmanchement sur ladite partie terminale 2 d'un cône de prélèvement 3, dont l'espace intérieur 4 est conformé avec une section longitudinale tronconique correspondante dans la zone où le contact avec l'embout 1 doit avoir lieu. Ce contact doit présenter la meilleure étanchéité possible compatible avec une éjection aisée du cône 3 lorsque celui-ci doit être remplacé. Dans le cadre de l'invention, le cône 3 peut avoir une configuration et des dimensions tout à fait habituelles, par exemple une surface intérieure strictement cylindrique ou tronconique, sans creux ou reliefs. Il n'y a pas, non plus, d'exigences particulières quant à son matériau constitutif, qui peut être de toute nature connue pour convenir à la fabrication des cônes de pipettes.The tip example 1 according to the invention shown in Figure 1 has, as is known, in its lower end part 2, a generally frustoconical outer shape so as to allow the fitting on said end part 2 of a sampling cone 3, the interior space 4 of which is shaped with a corresponding frustoconical longitudinal section in the zone where contact with the tip 1 is to take place. This contact must have the best possible seal compatible with easy ejection of the cone 3 when the latter must be replaced. In the context of the invention, the cone 3 may have a completely usual configuration and dimensions, for example a strictly cylindrical or frustoconical inner surface, without hollow or landforms. There are also no particular requirements as to its constituent material, which may be of any nature known to be suitable for the manufacture of pipette tips.
L'embout 1 selon l'invention peut être conçu pour équiper une pipette préexistante en se substituant simplement à un embout amovible de configuration classique. Il peut aussi constituer une extrémité non amovible d'une pipette qui, par ailleurs, serait de conception habituelle.The tip 1 according to the invention can be designed to equip a pre-existing pipette by simply replacing a removable tip of conventional configuration. It can also constitute a non-removable end of a pipette which, moreover, would be of the usual design.
Selon l'invention, la partie terminale 2 de l'embout 1 présente deux épaulements annulaires : un épaulement supérieur 5 et un épaulement inférieur 6. Ils procurent chacun à leur niveau une modification relativement brutale du diamètre extérieur de l'embout 1 , à savoir une diminution de ce diamètre en direction de l'extrémité inférieure 8 de l'embout 1. Ils viennent successivement en contact avec la paroi intérieure 7 du cône 3 lors de l'emmanchement du cône 3 sur l'embout 1 , et permettent d'assurer la fonction de maintien du cône 3 sur l'embout 1 avec une meilleure efficacité que lorsque, classiquement, la partie terminale 2 de l'embout 1 a un diamètre qui diminue constamment de façon progressive en direction de son extrémité inférieure 8.According to the invention, the end part 2 of the end piece 1 has two annular shoulders: an upper shoulder 5 and a lower shoulder 6. They each provide at their level a relatively sudden change in the outer diameter of the end piece 1, namely a reduction in this diameter in the direction of the lower end 8 of the nozzle 1. They successively come into contact with the inner wall 7 of the cone 3 when fitting the cone 3 on the nozzle 1, and allow ensure the function of maintaining the cone 3 on the end piece 1 with better efficiency than when, conventionally, the end part 2 of the end piece 1 has a diameter which constantly decreases progressively towards its lower end 8.
Ces épaulements 5, 6 correspondent chacun à une diminution de diamètre de l'embout 1 qui est de 0,2 à 2mm, de préférence de 0,3 à 1 ,8mm. De préférence, ces épaulements 5, 6 ne présentent pas d'angle vif mais sont arrondis pour faciliter l'emmanchement du cône 3. Typiquement, la diminution du diamètre de l'embout 1 correspondant à un épaulement 5, 6 est réalisée sur une longueur de 0,2 à 5mm, de préférence de 0,5 à 3mm.These shoulders 5, 6 each correspond to a reduction in the diameter of the end piece 1 which is from 0.2 to 2mm, preferably from 0.3 to 1.8mm. Preferably, these shoulders 5, 6 do not have a sharp angle but are rounded to facilitate the fitting of the cone 3. Typically, the reduction in the diameter of the end piece 1 corresponding to a shoulder 5, 6 is carried out over a length from 0.2 to 5mm, preferably from 0.5 to 3mm.
L'épaulement supérieur 5 a pour fonction essentielle de constituer à la fois un organe de centrage du cône 3 lors de l'emmanchement et une butée progressive. L'épaulement inférieur 6, lui, assure essentiellement l'étanchéité de la liaison cône 3/embout 1 , et c'est à son niveau que la déformation très localisée du cône 3 provoquée par l'emmanchement est la plus forte. La distance entre les deux épaulements 5, 6 est typiquement de l'ordre de 2 à 8mm, de préférence de 3,5 à 6mm. Les choix précis de ces dimensions dépendent essentiellement des caractéristiques des cônes qui seront utilisés. Comme représenté, le cône 3 peut, de manière classique, contenir un filtre 9 ; mais cette caractéristique, indépendante de l'invention, n'est, bien entendu, pas du tout obligatoire.The main function of the upper shoulder 5 is to constitute both a centering member of the cone 3 during fitting and a progressive stop. The lower shoulder 6, for its part, essentially ensures the tightness of the cone 3 / end piece 1 connection, and it is at its level that the very localized deformation of the cone 3 caused by the fitting is strongest. The distance between the two shoulders 5, 6 is typically of the order of 2 to 8mm, preferably 3.5 to 6mm. The precise choices of these dimensions depend essentially on the characteristics of the cones which will be used. As shown, the cone 3 can, conventionally, contain a filter 9; but this characteristic, independent of the invention, is of course not at all compulsory.
L'invention permet de fiabiliser la géométrie du contact cône 3 /embout 1, dont la qualité devient ainsi beaucoup moins dépendante que dans l'art antérieur de l'effort exercé par l'utilisateur lors de l'emmanchement du cône 3. Du fait, notamment, de l'existence de la butée constituée par l'épaulement supérieur 5, un effort d'emmanchement élevé ne conduit pas à un enfoncement de la partie terminale 2 de l'embout 1 dans le cône 3 sensiblement supérieur à celui que procure un effort d'emmanchement moindre.The invention makes it possible to make the geometry of the cone 3 / tip 1 contact more reliable, the quality of which thus becomes much less dependent than in the prior art on the force exerted by the user when fitting the cone 3. Due to the fact , in particular, the existence of the stop constituted by the upper shoulder 5, a high fitting force does not lead to a depression of the end part 2 of the end piece 1 in the cone 3 substantially greater than that provided by less fitting effort.
Une conséquence en est que lorsqu'on désire séparer le cône 3 de l'embout 1 , l'effort d'éjection qu'il est nécessaire d'effectuer prend généralement une valeur bien déterminée, comme le montre la figure 3. Celle-ci représente qualitativement et schématiquement l'effort d'éjection Faction qu'il est nécessaire d'exercer sur le cône 3, en fonction de l'effort Femmanchement qui a été exercé au cours de l'emmanchement du cône 3. Dans le cas d'un embout 1 dont la partie terminale 2 est conformée de la façon habituelle (courbe 10), l'effort d'éjection nécessaire augmente sensiblement linéairement avec l'effort d'emmanchement qui a été exercé. Mais dans le cas où on utilise un embout 1 dont la partie terminale 2 est conformée selon l'invention (courbe 11), du fait de la présence de l'épaulement supérieur 5 qui sert de butée au cône 3, il existe un effort d'emmanchement nominal Fn0m, correspondant à un effort d'éjection limite F|irn, au-delà duquel une augmentation de l'effort d'emmanchement n'a pas d'effet sur le position du cône. En conséquence, même pour un effort d'emmanchement supérieur à Fπom, l'effort d'éjection nécessaire demeure égal à Fπm. Il est donc aisé, rien qu'en appliquant un effort d'emmanchement supérieur à Fn0m, mais qui n'a pas besoin d'être déterminé plus précisément, de garantir qu'un effort d'éjection précis, égal à F|jm, sera nécessaire.A consequence of this is that when it is desired to separate the cone 3 from the nozzle 1, the ejection force which it is necessary to perform generally takes on a well-determined value, as shown in FIG. 3. qualitatively and schematically represents the ejection force Faction which it is necessary to exert on the cone 3, according to the force F emma nissement which was exerted during the fitting of the cone 3. In the case of a tip 1, the end part 2 of which is shaped in the usual way (curve 10), the necessary ejection force increases substantially linearly with the fitting force which has been exerted. But in the case where a tip 1 is used, the end part 2 of which is shaped according to the invention (curve 11), due to the presence of the upper shoulder 5 which serves as a stop for the cone 3, there is a force d nominal fitting F n0 m, corresponding to a limit ejection force F | irn , beyond which an increase in the fitting force has no effect on the position of the cone. Consequently, even for a fitting force greater than F π om, the necessary ejection force remains equal to Fπ m . It is therefore easy, just by applying a fitting force greater than F n0 m, but which does not need to be determined more precisely, to guarantee that a precise ejection force, equal to F | j m , will be necessary.
Concernant la zone d'étanchéité constituée par la zone de contact entre l'épaulement inférieur 6 et la paroi interne 7 du cône 3, sa surface est plus faible qu'avec la configuration lisse la plus habituelle de la partie terminale 2 de l'embout 1. La pression de contact à son niveau est donc plus élevée, ce qui est favorable à l'obtention d'une étanchéité fiable et reproductible. De plus, la position de cette zone d'étanchéité est bien maîtrisée. Ces avantages sont particulièrement importants dans le cas des pipettes multicanaux pour lesquelles, comme on l'a exposé précédemment, il est habituellement très difficile d'obtenir des conditions d'étanchéité identiques et assurément suffisantes au niveau de chaque cône. La figure 4 illustre ce fait. Elle montre qualitativement (courbe 12) la pression de contact Pcontact mesurée après l'emmanchement simultané de l'ensemble des cônes sur les 12 embouts d'une pipette multicanaux classique, au niveau des zones d'étanchéité de chacun des embouts. On voit que cette pression de contact est très variable d'un embout à l'autre et que les pressions les plus élevées sont atteintes, comme on le constate généralement, pour les embouts situés dans les zones latérales de la pipette. Sur les embouts situés dans la zone médiane de la pipette, on risque de ne pas atteindre la valeur minimale 13 de la pression de contact assurant une bonne étanchéité. La courbe 14 montre cette même pression de contact, mesurée au niveau des zones d'étanchéité de chacun des 12 embouts d'une pipette multicanaux dont les embouts sont conformés selon l'invention et dont les cônes sont identiques à ceux de la pipette classique précédente. On voit que : - cette pression de contact se situe à des valeurs sensiblement plus élevées que pour la pipette classique ; cela est dû, comme on l'a dit, à la plus faible surface de la zone d'étanchéité ;Regarding the sealing zone formed by the contact zone between the lower shoulder 6 and the internal wall 7 of the cone 3, its surface is smaller than with the most usual smooth configuration of the end part 2 of the end piece 1. The contact pressure at its level is therefore higher, which is favorable to obtaining a reliable and reproducible seal. In addition, the position of this sealing zone is well controlled. These advantages are particularly important in the case of multichannel pipettes for which, as explained above, it is usually very difficult to obtain identical and certainly sufficient sealing conditions at each cone. Figure 4 illustrates this fact. It qualitatively shows (curve 12) the contact pressure Pcont a c t measured after the simultaneous fitting of all the cones on the 12 tips of a conventional multichannel pipette, at the level of the sealing zones of each of the tips. It can be seen that this contact pressure is very variable from one tip to another and that the highest pressures are reached, as is generally observed, for the tips located in the lateral zones of the pipette. On the nozzles located in the middle zone of the pipette, there is a risk of not reaching the minimum value 13 of the contact pressure ensuring a good seal. Curve 14 shows this same contact pressure, measured at the level of the sealing zones of each of the 12 tips of a multichannel pipette whose tips are shaped according to the invention and whose cones are identical to those of the previous conventional pipette. . It can be seen that: - this contact pressure is situated at values which are appreciably higher than for the conventional pipette; this is due, as has been said, to the smaller surface area of the sealing zone;
- et que sa valeur est beaucoup plus homogène sur l'ensemble des canaux que pour la pipette classique. On est ainsi assuré d'obtenir une étanchéité satisfaisante pour tous les embouts.- and that its value is much more homogeneous on all the channels than for the classic pipette. This ensures that a satisfactory seal is obtained for all the end pieces.
Il serait envisageable de prévoir sur la partie terminale 2 de l'embout 1 plus de deux épaulements 5, 6 tels que décrits précédemment. L'étanchéité serait ainsi de même qualité et l'embout 1 tout aussi tolérant vis-à-vis de petits écarts sur les dimensions intérieures du cône par rapport aux dimensions nominales. L'embout de pipette selon l'invention peut d'adresser à des pipettes monocanal ou multicanaux. Les caractéristiques typiques de l'invention sont utilisables indifféremment dans les deux cas. Les autres caractéristiques de l'embout sont les caractéristiques habituelles les rendant adaptées à une utilisation sur l'un ou l'autre de ces types de pipettes. It would be conceivable to provide on the end part 2 of the end piece 1 more than two shoulders 5, 6 as described above. The seal would thus be of the same quality and the end piece 1 just as tolerant of small deviations on the internal dimensions of the cone with respect to the nominal dimensions. The pipette tip according to the invention can be used for single-channel or multi-channel pipettes. The typical characteristics of the invention can be used indifferently in the two cases. The other characteristics of the tip are the usual characteristics making them suitable for use on either of these types of pipettes.

Claims

REVENDICATIONS
1. Embout pour pipette de prélèvement, dont la partie terminale (2) sensiblement tronconique ou cylindrique est destinée à recevoir par emmanchement un cône de prélèvement (3), caractérisé en ce que ladite partie terminale comporte au moins deux épaulements annulaires (5, 6) aux niveaux desquels le diamètre de ladite partie terminale diminue en direction de l'extrémité inférieure (8) de l'embout, et en ce que lesdits épaulements procurent à leurs niveaux une diminution du diamètre de ladite partie terminale (2) de 0,2 à 2mm, préférentiellement 0,3 à 1 ,8mm. 1. Tip for sampling pipette, the end part (2) of which is substantially frustoconical or cylindrical is intended to receive by fitting a sampling cone (3), characterized in that said end part comprises at least two annular shoulders (5, 6) ) at which the diameter of said end part decreases towards the lower end (8) of the nozzle, and in that said shoulders provide at their levels a decrease in the diameter of said end part (2) of 0, 2 to 2mm, preferably 0.3 to 1.8mm.
2. Embout selon la revendication 1 , caractérisé en ce que lesdits épaulements (5, 6) sont arrondis.2. End piece according to claim 1, characterized in that said shoulders (5, 6) are rounded.
3. Embout selon la revendication 2, caractérisé en ce que lesdits épaulements (5, 6) s'étendent sur une longueur de 0,2 à 5mm, préférentiellement de 0,5 à 3mm. 3. End piece according to claim 2, characterized in that said shoulders (5, 6) extend over a length of 0.2 to 5mm, preferably 0.5 to 3mm.
4. Embout selon l'une des revendications 1 à 3, caractérisé en ce que la distance entre les épaulements (5, 6) est de 2 à 8mm, de préférence 3,5 à 6mm.4. End piece according to one of claims 1 to 3, characterized in that the distance between the shoulders (5, 6) is 2 to 8mm, preferably 3.5 to 6mm.
5. Embout selon l'une des revendications 1 à 4, caractérisé en ce qu'il est adapté à une utilisation sur une pipette multicanaux.5. Tip according to one of claims 1 to 4, characterized in that it is suitable for use on a multichannel pipette.
6. Pipette de prélèvement, caractérisée en ce qu'elle comporte au moins un embout (1 ) selon l'une des revendications 1 à 5.6. Sampling pipette, characterized in that it comprises at least one tip (1) according to one of claims 1 to 5.
7. Pipette selon la revendication 6, caractérisée en ce qu'il s'agit d'une pipette multicanaux. 7. Pipette according to claim 6, characterized in that it is a multichannel pipette.
EP06709248A 2005-02-18 2006-02-03 Tip for sampling pipette and pipette thus equipped Ceased EP1855804A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0501713A FR2882273B1 (en) 2005-02-18 2005-02-18 TIP FOR TAKING PIPETTE, AND PIPETTE THUS EQUIPPED
PCT/FR2006/000259 WO2006087444A1 (en) 2005-02-18 2006-02-03 Tip for sampling pipette and pipette thus equipped

Publications (1)

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EP1855804A1 true EP1855804A1 (en) 2007-11-21

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US (1) US8480972B2 (en)
EP (1) EP1855804A1 (en)
JP (1) JP4977626B2 (en)
KR (1) KR101236589B1 (en)
CN (1) CN101119803B (en)
AU (1) AU2006215489A1 (en)
CA (1) CA2598337C (en)
FR (1) FR2882273B1 (en)
MX (1) MX2007010056A (en)
RU (1) RU2388538C2 (en)
WO (1) WO2006087444A1 (en)

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US7662344B2 (en) * 2006-10-24 2010-02-16 Viaflo Corporation Locking pipette tip and mounting shaft
FR2920675B1 (en) 2007-09-10 2010-12-03 Gilson Sas MULTICHANNEL PIPETTING SYSTEM COMPRISING AN IMPROVED GUIDE PISTON HOLDER
WO2010082080A1 (en) * 2009-01-16 2010-07-22 Gilson S.A.S. Multi-channel pipette guidance system
US20150273463A1 (en) 2012-11-02 2015-10-01 Biotix, Inc. Pipetting system device
WO2014179256A1 (en) * 2013-04-30 2014-11-06 Corning Incorporated Pipette tip
WO2015198469A1 (en) * 2014-06-27 2015-12-30 株式会社 エー・アンド・デイ Pipette tip
JP1542088S (en) * 2015-03-16 2017-07-10
CA3165085C (en) 2020-02-14 2024-02-20 DeNovix, Inc. Dynamic broad volumetric range pipette

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Also Published As

Publication number Publication date
WO2006087444A1 (en) 2006-08-24
KR101236589B1 (en) 2013-02-22
MX2007010056A (en) 2007-10-16
JP4977626B2 (en) 2012-07-18
JP2008530573A (en) 2008-08-07
US20070297948A1 (en) 2007-12-27
CA2598337C (en) 2013-08-06
FR2882273A1 (en) 2006-08-25
US8480972B2 (en) 2013-07-09
CN101119803B (en) 2010-11-17
RU2388538C2 (en) 2010-05-10
AU2006215489A1 (en) 2006-08-24
CN101119803A (en) 2008-02-06
CA2598337A1 (en) 2006-08-24
RU2007134513A (en) 2009-03-27
FR2882273B1 (en) 2007-06-01
KR20070108545A (en) 2007-11-12

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