EP0788369A1

EP0788369A1 - AROMATIC COMPOSITION BASED ON OLIBANAM COMBINED WITH A SYNERGIC AGENT (e.g. EUCALYPTOL, BARNEOL, ZINC, SALT, COPPER, SALT) AND USE THEREOF

Info

Publication number: EP0788369A1
Application number: EP95934198A
Authority: EP
Inventors: Aurèle MANNARINI
Original assignee: Gombert Bernard
Current assignee: Gombert Bernard
Priority date: 1994-10-13
Filing date: 1995-10-11
Publication date: 1997-08-13
Also published as: AU3658395A; WO1996011694A2; CA2202554A1; CN1160354A; OA10414A; AP9700958A0; WO1996011694A3; FR2725621A1; FR2725621B1; AP707A; JPH11500466A

Abstract

The present invention concerns the general field or aromatic compounds derived, in particular, from essential oils or plant essences. An aromatic composition comprises at least one active olibanum aromatic system consisting of, firstly, at least one olibanum essential oil and/or one or more components thereof and, secondly, at least one synergic agent selected from the compounds of formula C10H180 and/or one metal selected from copper and zinc salts.

Description

Aromatic composition based on olibanum in combination with a synergistic agent (e.g. eucalyptol, barneol, zinc salt, copper salt) and its use

TECHNICAL AREA

The present invention relates to the general field of aromatic compounds contained, in particular, in essential oils or plant essences.

Such compounds obviously have the primary function of giving off pleasant and marked odors and tastes, so that some of them are used very naturally and very commonly in the field of food flavoring or perfumery. However, these compounds may also have therapeutic, antiseptic, disinfectant, deodorant, or even anti-parasitic and insecticide / insect repellant properties, these virtues possibly being used in industrial and commercial products.

More specifically, the subject of the present invention is an aromatic composition comprising at least one aromatic active ingredient based on olibanum constituted by at least one essential oil of olibanum or by one or more components thereof. The present invention also relates to the use of such a composition for the preparation of medicaments, sanitary and hygienic products, disinfectant, deodorant, antiseptic, insecticide, insect repellant, antiparasitic, phytosanitary, cosmetic products or even perfumes, among others.

PRIOR ART

Frankincense essential oil - olibanum - (frankincense) is extracted from plants of the Burseraceae family. These plants whose countries of origin are mainly those of North East Africa, Arabia as well as Iran, provide a gum - oleoresin which contains sugars, alcohols and acids. Conventionally, oil essential is obtained by steam distillation of this gum-oleoresin. The distillate consists of a viscous liquid which can be colorless, pale yellow, yellowish, reddish yellow depending on its source and the purification treatments to which it may be subjected. Frankincense is obviously used for its pleasant odoriferous and aromatic properties, which develop especially under the action of heat. Therapeutic applications of olibanum essential oil are also known:

- treatment of infectious conditions for internal and external use,

- use as an internal antidepressant, - use as a basis for the development of stimulants and balsamics,

- incorporation in pills of opiate cyanoglosses, in mercurial plasters or in preparations active against certain neuralgias.

And its pleasant odoriferous character means, of course, that it is used in perfumery. However, the industrial and commercial exploitation of essential oil of olibanum or its aromatic components has always remained extremely limited and discreet. Most of these pharmaceutical, cosmetic or perfume applications are no longer used today.

There are several reasons for this obsolescence, which will be examined below.

Firstly, essential oil of olibanum suffers from the drawback of being hardly soluble in conventional organic solvents, used in pharmacies or in perfumery. This considerably hinders the handling of this essence and its convenient incorporation, in different forms in various preparations. In addition, this lack of solubility has the direct consequence of less diffusion, both external and internal, in therapeutic use. This means that the bioavailability of the active ingredients of olibanum cannot be maximum and that, as a result, these therapeutic performances are affected.

Secondly, the essence of olibanum has an annoying tendency to deteriorate during its aging, in particular when it is exposed to aggressive environmental factors, of the type: air, light and heat. This results in a oxidation and by a "resinification", that is to say an increase in viscosity, which only complicates the handling and use of this essence. This degradation phenomenon, in particular by oxidation, is accompanied by the formation of undesirable by-products, having regard to the therapeutic, perfuming, deodorant, disinfectant or other effects sought.

Thirdly, it is quite obvious that the aromatic compositions of the olibanum type would have everything to gain, as regards their industrial and commercial interest, in the improvement of their functional, therapeutic, odoriferous, disinfectant and other properties. In this perspective, the final goal is to increase activity while reducing the dose, for obvious economic reasons.

STATEMENT OF THE INVENTION

In this state of the art, one of the essential objectives of the present invention is to provide an aromatic composition based on active olibanum compounds and having physicochemical properties, in particular improved solubility and aging stability and more adapted to the industrial requirements of simplicity of implementation and long storage life.

Another objective of the invention is to provide an aromatic composition based on Olibanum or the like, which has functional properties, in particular pharmaceutical, cosmetic, perfume or hygienic properties which are maximum at the lowest possible active doses.

Another object of the invention is to provide an aromatic composition which constitutes a raw material not only effective and economical, but also usable easily and universally, in multiple applications.

To meet these objectives, among others, the Applicant has had the merit of highlighting, in a completely surprising and unexpected manner and after numerous researches and experiments, that it is advisable to associate the active compounds of olibanum with at least one synergistic agent chosen from the compounds of formula

C ₁₀ H, _g O and / or metal salts. Thus, the present invention relates to an aromatic composition comprising, on the one hand, at least one active aromatic system based on olibanum and consisting of at least one essential oil of olibanum and / or by one or more components thereof and, on the other hand, at least one synergistic agent chosen from the compounds of formula CioHigO and / or from the metal salts of columns IB and LIB of the periodic table of the elements ("Chemistry - Encyclopedic Dictionary - Jacques Angenault - Editions DUNOD - 1991), zinc and copper being particularly preferred.

The advantage and the interest of this composition lies in the exacerbation of the physicochemical and functional properties of the active aromatic system based on olibanum, by this particular chemical class of aromatic and / or metallic synergistic compounds. This is reflected in particular by:

- a synergy of action,

better solubility in organic solvents and, in particular in ethanol, as well as in aromatic components,

- better dissemination,

- and greater stability in aging.

This original association is of definite industrial and commercial interest for all aromatic plant products from olibanum. The essential oil of olibanum-olibanum (frankincense) more particularly selected in accordance with the invention, is extracted from small trees of the burseraceae family; preferably: Boswellia carterii - birdw and related species. These small trees mainly grow in Iran, India, Africa and Arabia on both sides of the Red Sea and the Indian Ocean. On the African side, it is the horn of Africa, Ethiopia of Somalia, the island of Socotra and Eritrea where the boswellia carterii birdwood, boswellia frereana birdwood (or female incense are found) ) and boswellia bhau-dajiana birdwood (or incense mixed). Boswellia papyrifera (Del.) Hochst is thought to grow in Sudan and Eritrea. In Arabia we find the boswellia sacra flueckiger. In India, we find boswellia serrata Ro. All the boswellia which have just been cited above and which the Applicant has had the merit of isolating and selecting, provide an oleo-resin gum which contains sugars, acids, alcohols, an essential oil.

According to an original and advantageous arrangement of the invention, the components of the essential oil of frankincense are grouped together, according to their chemical structure, with a percentage varying according to geographical origins (Higazy, Obermann, Peyron and others).

- Hydrocarbons: α-pinene (constituent predominant in olibanum essential oil from Aden up to 40%), / 3-pinene, limonene, myrcene ...

- alcohols: olibanol, borneol, farnesol, octanol, incensol (Eritrea) ...

- ethers: cineole-1,8 - carbonylated: carvone, verbenone ...

- boswellic acids α and 3 ...

- esters: octyl acetate (predominant constituent: Eritrea, up to 50 T and more).

Borneol in the nascent or esterified state, as well as cineole are in small proportion in essential oil of olibanum more or less 1% on average in Eritrea and Aden.

There is an olfactory unit of gums-resins-incense and their products. extraction.

Several technologies can be used to extract the components of olibanum resins, e.g.: steam distillation, volatile solvents. thermolysates.

In accordance with the invention, these three techniques are preferred, but steam distillation of this oleoresin gum is particularly preferred. The distillate consists of a viscous liquid which can be colorless, pale yellow, yellowish, reddish yellow depending on the source and the treatments to which it may be subjected. The density of this frankincense essence varies from about 0.850 to 1060. It is all the heavier when it is resinated or it is resinous.

In accordance with a preferred characteristic of the invention, the synergistic agent is at least partly of aromatic nature and comprises eucalyptol, borneol (native or in the form of an ester) or a mixture of them. These two compounds are C ₁₀ Hι ₈ O isomers, widely available and relatively inexpensive. Eucalyptol, also known as cineole-1,8 or epoxy-1,8p-menthane, is an internal ether oxide corresponding to terpine. It is extractable, in liquid form, from various plant essences. It is of course those of eucalyptus which contain the greatest amount, namely up to 85%. By way of example, there may be mentioned: Eucalyptus globulus, Eucalyptus austrialana, Eucalyptus amygdalina,

Eucalyptus polybractea, and Eucalyptus smithii, among others.

We can also mention Malaleuca leucadendron and Malaleuca viridiflora which contain respectively 60 to 75% and 35 to 70% of Eucalyptol. For the record, we will finally mention Artemisa cina and Artemisa vulgaris. Eucalyptol is a mobile, colorless liquid whose smell resembles both mint and camphor, its density is 0.921. The known pharmacological activities of eucalyptol are those of a powerful expectorant and antiseptic. It is mainly used as a means of combating infections of the respiratory and genitourinary systems in particular. There are also uses as a disinfectant, deodorant, antiparasitic, insecticide / insect repellent.

Eucalyptol useful as a synergistic agent in the context of the invention may be in pure form or be provided by an essential oil, which is rich in it, for example by one or more of those mentioned above. With regard to borneol, it can be noted that it is an alcohol supplied by a tree growing in Borneo and Sumatra: Dryobalanops Camphora. Borneol (straight camphol), also known as 1,7,7-trimethylbicyclo [2.2.1] heptane-2-ol, is collected in crystalline form under the bark of Dryobalanops camphora.

Borneol is soluble in ethanol and fluid plant essences. Its density is less than that of water. Various plant essences contain it, either in its pure state, or in the form of an ester. By way of illustration of these source essences of borneol, we can cite:

- Rosmarinus Officin lily (15% weight)

- essential oils of pine, and more particularly: * Abiès sibirica (40% by weight of bornyl acetate)

* Tsuga canadensis, * Pinus silvestris,

* Pinus mariana etc ..

- Origanum majorana (85% weight of a mixture of camphor and borneol "Bruylants") - Thymus satureoides (35% weight).

Borneol is an antiseptic and, for many authors, a general tonic and more specifically a stimulant of the adrenal cortex. It is used in the treatment of infectious diseases and depressive states, or even as a desensitizer. In the same way as for eucalyptol, the addition of a synergistic agent based on borneol in the aromatic composition of the invention can be carried out by means of one or more essential oils, including in particular those mentioned above. and which are rich in this active aromatic compound.

Since, in order to obtain the aromatic compositions according to the invention, it is possible, in particular, to have recourse to all or part of essential oils of olibanum as well as to essential oils containing eucalyptol and / or borneol, it is interesting to specify that these plant essences can present variations in their compositions, said variations depending on several factors. First of all, it is obvious that the vegetable and living origin of these oils determines a variability due to the geographical location, on which the climate and the nature of the soil depend. In addition, changes in composition may occur depending on the time of harvest of the plant. Finally, it is clear that the distillation and the manner in which it is carried out are other factors which somewhat influence the essential oil obtained in fine. This considered, it should be noted that the first factor is not really crucial and that it is possible to control the other two, so as to ensure consistency of the properties, in particular physicochemical, therapeutic and cosmetic, of essential oils .

It is also to be considered that the latter can be in rectified form or not for their use in the aromatic compositions according to the invention. Thus, within the meaning of the present invention, the term "rectified" refers to one or more purification treatments, by means of which the essential oils are rid of one or more of their undesirable components. In particular, terpenes, which are present in virtually all essential oils, can be removed by deterpenation. Such treatment aims to improve the solubility of petrol in ethanol, as well as its aromatic power. This relates more specifically to perfumery. It goes without saying that the purification or rectification of the essence can also extend to other constituents of the essences than the terpenes, namely: the alkaloids, or other non-terpene component.

These purification / rectification treatments make it possible to remove the annoying compounds according to the intended use. These annoying compounds can thus be eliminated completely or partially.

In the case where the active olibanum-based system consists of at least one essential oil of olibanum and where the synergistic agent comprises eucalyptol, the latter is preferably present at 15 to 75%, preferably 25 to 35% by volume relative to the total composition.

On the other hand, when the olibanum-based active aromatic system consists of at least one olibanum essential oil and when the synergistic agent comprises borneol, the latter has a content of 5 to 100% by weight, preferably 20 at 30 Te by weight, and more preferably still around 25% by weight, relative to the active system based on olibanum.

Advantageously, the composition comprises at least one organic solvent chosen from fatty substances, alcohols, ethers or their mixtures, preferably from among alcohols and / or ethers, ethanol being more particularly preferred.

According to an advantageous variant of the invention, the synergistic agent comprises at least one metal salt present in an amount such that the metal cation (s) represent (s) from 0.1 to 10% by weight relative to the total mass of the aromatic compounds forming the aromatic body preferably 0.1 to 5%. Preferably, the synergistic agent comprises at least one zinc salt and / or at least one copper salt. In the case where the cation comes from copper, its concentration, expressed in% by weight relative to the total mass of the aromatic compounds (or aromatic body), is advantageously between 0.3 and 1%, preferably between 0.4 and 0.6%. In the case where the cation comes from zinc, its concentration, expressed in% by weight relative to the total mass of the aromatic compounds (or aromatic body), is advantageously between 0, 1 and 2%, preferably between 0.5 and 2%.

The synergistic combination of olibanum-based system, on the one hand, and eucalyptol and / or borneol and optionally Zn and / or Cu salt, on the other hand, has the advantageous effect of increasing the solubility of olibanum compounds in organic solvents, such as for example ethanol. This is particularly true for essential oil of olibanum, whether in rectified form or not and regardless of the extraction procedure.

It also turns out that the composition diffuses much better, compared to the aromatic composition based on olibanum alone, through various solid supports, such as animal or plant tissues, fibrous materials (when it is at liquid or gaseous state) and through gases, such as air (when in the vapor state).

This excellent diffusion is the source of better performance in terms of bioavailability, therefore therapeutic efficacy, but also in terms of perfumery, cosmetics, disinfection or deodorization of ambient air.

It has also been found that the synergistic combination described above provides particularly optimized storage stability properties. The invention can be implemented according to different embodiments. - a - According to a first exemplary embodiment, olibanum essential oil, whether rectified or not, is mixed with pure (or substantially pure) synergistic agent of the eucalyptol and / or borneol type. We can thus have for example:

- 30% by volume of essential oil of olibanum (rectified or not); - and 70% by volume of eucalyptol; or even : - 75% by weight of essential oil of olibanum (rectified or not),

- and 25% by weight of borneol, or finally:

- 30% by volume of essential oil of olibanum (rectified or not), - 70% by volume of eucalyptol,

- and 25% by weight of borneol relative to essential oil of olibanum. - b - According to a second exemplary embodiment, the composition may consist of a mixture of essential oils constituting a vehicle for the essential active ingredients of the invention, namely: olibanum compounds and the synergistic agent based on eucalyptol and or borneol and optionally at least one metal salt. In this case, the essential active ingredients of the invention (olibanum and synergistic agents) naturally remain present in the same proportions as those mentioned above. In practice, this can, for example, be a mixture of olibanum essential oils, rectified or not, with essential oils, rectified or not, rich in eucalyptol and / or borneol and, preferably, those mentioned above.

Regarding essential oils rich in borneol, you can choose those that contain borneol in their native state. However, the latter has particularly advantageous solubilizing properties, compared to borneol in the form of esters, the performance of which, on the other hand, can be exploited in terms of therapeutic properties.

It is thus possible to adjust the respective contents of native bornéol and bornéol esters, according to the intended end applications:

+ in a first embodiment, the therapeutic properties (synergy) can be favored by enriching the composition in borneol in the form of esters,

+ in a second embodiment, the solubilizing properties (perfumery) can be exploited by increasing the quantity of native borneol,

+ and in a third embodiment, we can use two types of properties. - c - Without it being so! limiting and as a third exemplary embodiment, we can also mention the alternative according to which one selects one or several components of olibanum essence including alcohols such as olibanol and acids. To this end, fractional distillation can be used as well as other purification methods, which allow deterpenation, the consequences of which are a limitation in the amount of the components and an increase in the proportion of acids and alcohols or of a preferred component, such as for example octanol in the elementary state or in the form of an ester such as octyl acetate in the essence of olibanum from Eritrea.

In such an alternative, it is advantageously associated with the component (s) of essential oil of olibanum, either eucalyptol, or a mixture of eucalyptol and borneol (in pure form or in the form of oil (s) essential).

The component (s) thus represent (s), preferably, between 5 and 60% by weight of a composition comprising the component (s) and eucalyptol, the concentration of 15% by weight being particularly preferred.

Preferably, the associated synergistic agent is eucalyptol; but this does not exclude mixtures of eucalyptol / bomeol. In these latter cases, the borneol concentration is between 10 and 50% by weight relative to the mixture of eucalyptol and of the olibanum component (s) chosen, preferably 25% by weight. This corresponds to e.g.:

- component (s) from essential oil of olibanum (15% by weight),

= χ - eucalyptol (85% by weight),

- possibly borneol 25% by weight of x. Given . that the total essence of olibanum is all the more soluble in organic solvents such as ethanol that it is resinous,. and that the latter tends to disappear with the rectified essence, it is conceivable to use total olibanum essential oil that is not rectified and resinous to determine maximum solubility. This improves the therapeutic properties of the composition.

Conversely, it is possible, in particular, for an application in perfumery, to use a non-resinous and rectified olibanum essential oil, and for a increased diffusion and stability and better solubility in organic solvents, to associate it with borneol and / or eucalyptol.

Within the framework of the advantageous variant of the invention mentioned above, the activity of the essential oil of olibanum and / or of one or more components of this same is reinforced with at least one metal salt. essential oil, as well as their association with borneol and / or eucalyptol. The preferred metals are zinc and copper. They appear in the pharmacopoeia as sedative of the nervous system (neuralgia, epilepsy, spasms) and antiseptics. They have the same therapeutic aim as the aromatic components with which it can be associated. And even better, they therefore act in synergy with these aromatic components. The originality consists in associating, for example, one (or two) metal via a mineral salt like zinc chloride or cupric chloride or an organic salt like zinc acetate and copper acetate . The percentage of the elemental metal (or metals) is between 0, 1 and 10% by weight of the aromatic body consisting of essential oil of olibanum or one or more of its components associated with borneol and / or eucalyptol . The percentage of zinc is preferably between 0.5 and 2% and more preferably 1.4% by weight. The percentage of elemental copper is preferably between 0.3 and 1% and more preferably 0.5% by weight. In practice, according to this alternative embodiment, the two metals are combined with the aromatic body. The percentage of the sum of the two metals (elemental metal) is preferably less than or equal to 2% by weight of the aromatic body.

Frankincense essential oil can thus act as an excipient or an intermediate excipient by diluting the borneol and the metal salts which are in the solid state; the whole forming a stable product.

The composition according to the invention, which combines essential oil of olibanum or one or more of its components with eucalyptol and (or) borneol as well as a zinc salt and (or) a copper salt can be used pure or with an aromatic or non-medicated, cosmetic or hygienic additive. In this case, the different compositions have a complementary role.

Indeed, for example during a combination therapy, it is easier to have recourse to a single preparation rather than to several. In this case, several compositions are in the same solution or come in the same dosage form. A pharmaceutical manufacturer interested in the present invention and wishing to associate it with an aromatic drug or not, already existing or future, can incorporate it in the same preparation with said drug. The same is true for a manufacturer of cosmetic and hygiene products.

The composition according to the invention with an additive or not, can be used with excipients, preferably fatty substances, more preferably ethers and alcohols, ethanol being preferred, as well as various preparations and pharmaceutical forms.

Advantageously, the compositions according to the invention can be added with at least one other aromatic ingredient or not.

INDUSTRIAL APPLICATION

According to another of these aspects, the subject of the invention is the use of the composition defined above, in particular in the preparation of drugs (eg anti-infectives, antiseptics, anti-depressants, stimulants, desensitizers) for internal use and or external (as the case may be), sanitary and hygienic products, disinfectant, deodorants, cosmetic products, perfumes or phytosanitary products.

Finally, the present invention relates, by way of new products e.g.: + drugs (e.g. anti-infectives, antiseptics, anti-depressants, desensitizing stimulants), + perfumes,

+ cosmetic products,

+ sanitary and hygienic products (deodorants, disinfectants), comprising the aromatic composition as described above. BEST WAY TO IMPLEMENT THE INVENTION

The examples which follow will make it possible to better understand the invention and to highlight all of these advantages and these various non-limiting variants of implementation.

EXAMPLES:

Examples 1 to 4: Solubility

> In Example 1, the following preparation No. 1 is carried out: - a - essential oil of Olibanum (Saudi origin) 100 g

- b - dissolved borneol 50 g by stirring for 5 min at room temperature (preferably after slightly heating the tube).

20 cm ³ of alcohol (ethanol) at 70 ° dissolve 1 cm ³ of preparation n ° 1, while the same quantity of essential oil - a - as that contained in 1 cm ³ of preparation n ° 1 is not not dissolved in 20 cm ³ of ethanol at 70 °.

> In Example 2, the following preparation No. 2 is carried out:

- c - essential oil of Olibanum rectified (origin Iran) 100 gr

- d - bornéol 50 gr, proceeding in the same way as in example 1.

40 cm ³ of alcohol at 70 ° dissolve 1 cm ³ of preparation No. 2. The same observation is made as in Example 1, of non-solubility in ethanol of gasoline - c - without borneol.

In general, borneol allows better solubility of essential oil of Olibanum whether or not it is corrected, regardless of its origin.

> In Example 3, the following preparation No. 3 is carried out, in volume:

- e - essential oil of Frankincense (Saudi origin) 30%

- f - eucalyptol 70%. in the same way as in Examples 1 and 2. Ten (10) volumes of 60 ° ethanol of this preparation No. 3 dissolve it.

For the essential oil used alone, it takes 80 times its volume of ethanol to 60 ° to dissolve it.

Whatever its origin, rectified or not, eucalyptol remarkably solubilizes essential oil of Frankincense.

Eucalyptol increases the solubility of Frankincense essential oil in ethanol.

> In example 4, the following prepaπration n '4 is carried out:

- g - essential oil of Olibanum supplied by the MANE Laboratory, origin Ethiopia, lot n ^* 95/01973 1 ml.

- h - pharmacy-type ethanol and 90% 0.6 ml.

After stirring, a cloudy mixture is obtained.

4.1 The addition of eucalyptol (French Pharmaceutical Cooperation lot no ^. A25565) in 10 μ \ steps makes it possible to obtain a clear, slightly colored solution. The total amount of eucalyptol added is 30 μi, i.e. 3% by volume compared to the olibanum oil used.

4.2 According to a variant, adding to the starting mixture of borneol (Laboratoire de Combe d'Ase, lot n "HB11) causes a clarification of the starting mixture, after addition of 5 mg of borneol, and the complete solubilization of the oil to 8 g of borneol (clear solution) 4.3 According to another protocol, olibanum resin (Laboratory of Combe d'Ase, batch n ^* HE02) is added to the starting mixture in the form of chips weighed at room temperature. The addition of 10 mg of resin does not remove the turbidity of the starting mixture, but heating to about 40 ° C. allows complete solubilization to be obtained. The mixture becomes cloudy again after cooling to room temperature. another 10 mg of resin, the mixture clears up and a solution is obtained by very slight heating, the solution then remaining clear at room temperature. Finally, 20 mg of resin was added to dissolve 1 ml of essential oil.

Example 5: Oxidation of the three mixing tests comprising essential oil of solubilized olibanum from Example 4.

During the 48 hours which followed the three tests 4.1. 4.2. and 4.3. of Example 4, the solubilized mixtures obtained were brought into contact with air by repeated opening of the bottles containing them. It was finally observed that the mixtures containing 1 ml of olibanum essential oil 0.6 ml of 90% ethanol and a synergistic agent formed by eucalyptol (test 4.1), borneol (test 4.2 ) and olibanum resin (test 4.3), give clear non-oxidized solutions. It therefore turns out that the oxidation of these mixtures does not disturb them, in particular as regards the solubilization of essential oil of olibanum. The NMR spectra of the mixtures before and after oxidation show slight differences.

Example 6: Preparation of a composition of essential oil of olibanum with synergistic agents of aromatic and metallic nature The following composition is prepared: As an example of embodiment of this variant, the following will be cited: aromatic body (molecules - Essential oil of olibanum 30% by aromatic volume) of = - Eucalyptol 70% by volume composition representing lg - Borneol 25% by weight relative to essential oil of olibanum

- zinc chloride 0.030 g - cmvπque chloride. . 0.015 g.

Example 7: Stability

By stability is meant here constant homogeneity of the liquid composition (without formation of precipitates). To appreciate this, hydrochloric acid (HC1) is made to act on Olibanian essential oil. This causes hyper-oxidation, which causes the essence to go from red to dark brown, as well as a characteristic odor. A precipitate is formed of the most oxidized components, as well as those chlorinated.

The test protocol is as follows: - 1/20 th of cm ³ of a 1/3 aqueous HCl solution is poured into 1 cm ³ of olibanum HE. It is stirred for a few seconds and left to stand for a few minutes. The aqueous solution is then separated from the supernatant oxidized essential oil. The aqueous solution is the same red color as the oxidized essential oil, because it contains oxidized and chlorinated products. The oxidized essential oil collected contains a precipitate which is all the more important as it is resinous.

In the hydrochloric acid (HC1) test, the fact of combining borneol or eucalyptol with Olibanum essence leads to the solubilization of the precipitate.

COMMENTS

It appears that eucalyptol and borneol stabilize Olibanum essential oil by dissolving its components and its oxidized and decomposing products.

The phenomenon of solubilization in ethanol of the decomposition products of olibanum essence, using borneol and eucalyptol, is also verified when the composition comprises certain non-rectified essences, as well as certain components of essential oils other than olibanum essence (aromatic additives).

Example 8: Diffusibility

A drop of eucalyptol on a sheet of paper causes a task that quickly grows and evaporates without leaving a trace.

A drop of Olibanum essential oil causes a task that increases slowly and leaves a trace like fixed oils. It seems that this is a specificity of olibanum essential oil compared to other essences. The task spreads more slowly the more essential oil of Olibanum is resinous; a deposit is then formed, the surface of which is less than that of the task.

By repeating the previous experiment in the presence of eucalyptol, it is found that the essence of Olibanum diffuses more quickly, and, for the same resinous essential oil, the surface of the deposit is larger.

Eucalyptol acts as a vehicle. This is important, in particular, for therapeutic use in internal and external use.

Claims

CLAIMS:

1 - Aromatic composition comprising,

on the one hand, at least one active aromatic system based on olibanum and consisting of at least one essential oil of olibanum, and / or by one or more components thereof, and

- on the other hand, at least one synergistic agent chosen from the compounds of formula C ₁₀ H 0, and / or from the metal salts of columns IB and IIB of the periodic table of elements ("Chemistry - Encyclopedic Dictionary - Jacques Angenault - Editions DUNOD - 1991), zinc and copper being particularly preferred.

2 - Aromatic composition according to claim 1, characterized in that the synergistic agent is at least partially aromatic and comprises eucalyptol, borneol or a mixture of them. 3 - Aromatic composition according to claim 1 or claim 2, characterized in that the synergistic agent is provided via at least one essential oil, which is rich in it, said essential oil being in rectified form or not.

4 - Composition according to any one of claims 1 to 3, characterized in that the active aromatic system based on olibanum consists of at least one essential oil of olibanum, and in that the synergistic agent comprising l eucalyptol is present in an amount of 15 to 75% by volume, preferably 25 to 35% by volume of the total composition.

5 - Composition according to any one of claims 1 to 4, characterized in that the active aromatic olibanum system consists of at least one essential oil of olibanum and in that the synergistic agent comprises borneol and in that the latter titer from 5 to 100% by weight, preferably 20 to 30% by weight, and even more preferably still about 25% by weight relative to the active olibanum-based system. 6 - Composition according to any one of claims 1 to 3, characterized in that the active aromatic system based on olibanum is formed by one or more components of essential oil of olibanum, in that it contains eucalyptol and possibly borneol as a synergistic agent, and in that it is quantitatively defined as follows:

- 1 - 15% by weight approximately of component (s) coming from essential oil of olibanum;

- 2 - approximately 85% by weight of analyptol, the borneol possibly being added up to approximately 25% by weight relative to the mixture 1 + 2.

7 - Composition according to any one of claims 1 to 6, characterized in that the synergistic agent comprises at least one zinc salt and / or at least one copper salt, and in that the metal cations of this (or ces) salt (s) represents 0.1 to 10% by weight of the total mass of the aromatic body, preferably 0.1 to 5%.

8 - Composition according to any one of claims 1 to 7, characterized in that it comprises at least one organic solvent chosen from fatty substances, alcohols, ethers or their mixtures, preferably from alcohols and / or ethers, ethanol being more particularly preferred.

9 - Composition according to any one of claims 1 to 8, characterized in that it is added with at least one other aromatic ingredient or not. 10 - Use of the composition according to any one of claims

1 to 9 in the preparation of medicines, sanitary and hygienic products, cosmetic products, perfumes, disinfectants, deodorants or phytosanitary products.