EP0127841A1 - Methode zum Färben mit Silbersalzen - Google Patents
Methode zum Färben mit Silbersalzen Download PDFInfo
- Publication number
- EP0127841A1 EP0127841A1 EP84105847A EP84105847A EP0127841A1 EP 0127841 A1 EP0127841 A1 EP 0127841A1 EP 84105847 A EP84105847 A EP 84105847A EP 84105847 A EP84105847 A EP 84105847A EP 0127841 A1 EP0127841 A1 EP 0127841A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- solution
- silver
- silver staining
- molecular weight
- polyethylene glycol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000002109 Argyria Diseases 0.000 title claims abstract description 18
- 238000007447 staining method Methods 0.000 title claims abstract description 6
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 14
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 14
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 11
- -1 polyoxyethylene Polymers 0.000 claims abstract description 10
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 9
- 239000003513 alkali Substances 0.000 claims abstract description 8
- 239000003518 caustics Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 239000000126 substance Substances 0.000 claims abstract description 7
- 230000001476 alcoholic effect Effects 0.000 claims abstract description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 18
- 229910021529 ammonia Inorganic materials 0.000 claims description 9
- 125000002924 primary amino group Chemical class [H]N([H])* 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 19
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 239000000499 gel Substances 0.000 description 17
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
- 239000003153 chemical reaction reagent Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 239000012192 staining solution Substances 0.000 description 13
- 238000005406 washing Methods 0.000 description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- 239000012153 distilled water Substances 0.000 description 11
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 8
- 238000001962 electrophoresis Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 150000001298 alcohols Chemical class 0.000 description 5
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052709 silver Inorganic materials 0.000 description 4
- 239000004332 silver Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
- KDSNLYIMUZNERS-UHFFFAOYSA-N 2-methylpropanamine Chemical compound CC(C)CN KDSNLYIMUZNERS-UHFFFAOYSA-N 0.000 description 2
- QUSNBJAOOMFDIB-UHFFFAOYSA-N Ethylamine Chemical compound CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- HQABUPZFAYXKJW-UHFFFAOYSA-N butan-1-amine Chemical compound CCCCN HQABUPZFAYXKJW-UHFFFAOYSA-N 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- WGYKZJWCGVVSQN-UHFFFAOYSA-N propylamine Chemical compound CCCN WGYKZJWCGVVSQN-UHFFFAOYSA-N 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- NDVLTYZPCACLMA-UHFFFAOYSA-N silver oxide Chemical compound [O-2].[Ag+].[Ag+] NDVLTYZPCACLMA-UHFFFAOYSA-N 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- HXKKHQJGJAFBHI-UHFFFAOYSA-N 1-aminopropan-2-ol Chemical compound CC(O)CN HXKKHQJGJAFBHI-UHFFFAOYSA-N 0.000 description 1
- BLFRQYKZFKYQLO-UHFFFAOYSA-N 4-aminobutan-1-ol Chemical compound NCCCCO BLFRQYKZFKYQLO-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206672 Gelidium Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
- WUGQZFFCHPXWKQ-UHFFFAOYSA-N Propanolamine Chemical compound NCCCO WUGQZFFCHPXWKQ-UHFFFAOYSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- CBTVGIZVANVGBH-UHFFFAOYSA-N aminomethyl propanol Chemical compound CC(C)(N)CO CBTVGIZVANVGBH-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 229940102253 isopropanolamine Drugs 0.000 description 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 229910001923 silver oxide Inorganic materials 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
Definitions
- This invention relates to silver stain and more particularly, to a method for staining a carrier, which bears thereon substances being detected, with silver in which the carrier is pretreated with an neutral or approximately neutral alcoholic solution comprising polyethylene glycol or a polyoxyethylene alkylphenol and subsequently treated with a silver staining solution of a specific type.
- a silver staining method which comprises pretreating a carrier bearing thereon a substance to be detected with an alcoholic solution comprising alcohol and water and containing polyethylene glycol or polyoxyethylene alkylphencl; and treating the pretreated carrier with a solution comprising silver nitrate, a compound of the formula R-NH 2 (R-NH 2 has the same meaning as defined above) and a caustic alkali in ratios by molecular weight of 1 : 4.5 - 9.5 : 1.0 - 26.0.
- a sole figure is a band image obtained in Example 3 in which indicated by a is a band image of BSA, by 1, 2, 3, 4, 5 and 6 are respectively, images obtained by electrophoresis of 5 ng, I ng, 500 pg, 100 pg, 50 pg and 10 pg of BSA.
- a carrier which has been subjected to electrophoresis by a usual manner (hereinafter referred to simply as gels) is immersed in an alcoholic solution containing polyethylene glycol or a polyoxyethylene alkylphenol (hereinafter referred to as washing and fixing solution) for about 10 to 60 minutes, preferably about 25 minutes, thereby causing the gels to be shrunk. By the shrinkage, the water and the reagent are released from the gels.
- the alcohols used in the washing and fixing solution include lower alcohols and preferably linear or branched alcohols having from 1 to 4 carbon atoms.
- linear or branched alcohols examples include methanol, ethanol, propanol, isopropanol, butanol, isobutanol, and mixtures thereof. Of theses, isopropanol, or a mixture of methanol and ethanol is preferred. These alcohols are conveniently used as an aqueous solution having a concentration of from 30 to 70 (v/v)%, preferably about 50 (v/v)%.
- Polyethylene glycol suitable for the purpose of the invention has an average molecular weight of from 600 to 3500, preferably 1500 to 2500.
- the polyoxyethylene aklylphenol is represented by the formula, R' in which R' represents a linear or branched alkyl having from 6 to 10 carbon atoms, and n is an integer of 7 to 10 which indicates the center of the distribution.
- R' is octyl and n which indicates a center of the distribution is 9.
- the polyethylene glycol (PEG) or polyoxyethylene alkylphenol is generally used in an concentration of 0.05 to 5 (w/v)%, preferably about 2 (w/v)%, of the washing and fixing solution.
- the gels pretreated with the washing and fixing solution are subsequently immersed in and treated with a silver staining solution for 10 to 30 minutes, preferably about 15 minutes.
- the silver staining solution used for the above treatment is a mixture of silver nitrate, a compound of the formula R-NH 2 (R-NH 2 has the same meaning as defined before), and a caustic alkali in mixing ratios by molecular weight of 1 : 4.5 - 9.5 : 1.0 - 26.0.
- the treatment with the silver staining solution within the above mixing ratios permits staining in good reproducibility and shortening of the operation time.
- Compounds of the formula, R-NH 2 usable in the present invention include, for example, ammonia, ethanolamine, propanolamine, isopropanolamine, butanolamine, isobutanolamine, tris(hydroxymethyl)aminomethane, methylamine, ethylamine, propylamine, butylamine, isobutylamine, etc.
- Examples of the caustic alkali include sodium hydroxide, potassium hydroxide, etc.
- the gels treated with the silver staining solution is then immersed in a developer solution for about 10 to 15 minutes. If necessary, after treatment with the developer, it may be further immersed in a solution of 0.5 to 2 (w/v)% chloroauric acid, by which a clearer image is obtained.
- the method of the present invention is conveniently applied to polymerized gels having the network structure, such as polyacrylamide gel, agar- agar, agarose, etc.
- the method of the invention ensures formation of a clear image in about 60 minutes. Moreover, the same procedure is applied to any substances contained in gels such as proteins, nucleic acids, sugars, lipids, etc.
- the method of the invention has the following features:
- Silver nitrate, ammonia and sodium hydroxide were dissolved in distilled water in ratios by molecular weight of 1: 7: 4.3 to make a total volume of 200 ml.
- citric acid 0.05% citric acid, 37% formalin and distilled water were used to make 200 ml of a 0.02 v/v% formalin solution of 0.005 w/v% citric acid.
- the evaluation standard is the same as in
- Isopropanol and polyethylene glycol 2000 were diluted-with distilled water to make a total volume of 200 ml with concentrations of 50%(v/v) and l%(w/v), respectively.
- Silver nitrate, ammonia and sodium hydroxide were dissolved in distilled water to have mixing ratios by molecular weight of 1: 4.8: 1. The total volume was brought up to 200 ml.
- Reagents (1), (2) and (3) were, respectively, placed in pan of the cabinet size.
- the sample was first immersed in the pan of reagent (1) for 25 minute and removed from the pan, followed by immersing in the reagent (2) thereby causing the silver staining solution to penetrate into the gel.
- the sample was immersed in a pan containing 200 ml of distilled water and washed. This procedure was repeated three times. Finally, the washed sample was immersed in the reagent (3) for 15 minute to form a brown to black band image.
- Methanol, ethanol and polyethylene glycol 2000 were diluted with distilled water to make a total volume of 200 ml with concentrations of 40 %(v/v), 10 %(v/v) and 2%(w/v), respectively.
- Silver nitrate, ammonia and sodium hydroxide were dissolved in distilled water to have mixing ratios by molecular weight of 1: 7: 4.3. The total volume was brought up to 200 ml.
- Example 3 The operation of Example 3 was repeated using the reagents indicated above, thereby obtaining band image similar to the image of Example 3.
- Isopropanol and polyoxyethylene phenol (commercial name of Rhom Haars Co., Ltd., of U .S. A .: Nonizet P -40) were diluted with distilled water in concentrations of 50 %(v/v) and 3 %(v/v) to make a total volume of 200 ml.
- Silver nitrate, ammonia and sodium hydroxide were dissolved in ratios by molecular weight of 1: 9.1: 25.5 to make a total volume of 200 ml.
- Example 3 The procedure of Example 3 was repeated using the reagents indicated above, thereby obtaining band image similar to the image of Example 3.
- Silver nitrate, tris(hydroxymethyl)aminomethane and sodium hydroxide were dissolved in distilled water in ratios by molecular weight of 1: 5: 1.0 to make a total volume of 200 ml.
- Example 3 The procedure of Example 3 was repeated using the reagents indicated above, thereby obtaining a band image similar to the image of Example 3.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AT84105847T ATE36195T1 (de) | 1983-05-26 | 1984-05-22 | Methode zum faerben mit silbersalzen. |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58091487A JPS59217164A (ja) | 1983-05-26 | 1983-05-26 | 銀染色方法 |
| JP91487/83 | 1983-05-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0127841A1 true EP0127841A1 (de) | 1984-12-12 |
| EP0127841B1 EP0127841B1 (de) | 1988-08-03 |
Family
ID=14027765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP84105847A Expired EP0127841B1 (de) | 1983-05-26 | 1984-05-22 | Methode zum Färben mit Silbersalzen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US4582808A (de) |
| EP (1) | EP0127841B1 (de) |
| JP (1) | JPS59217164A (de) |
| AT (1) | ATE36195T1 (de) |
| DE (1) | DE3473177D1 (de) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0359281A2 (de) * | 1988-09-16 | 1990-03-21 | Daiichi Pure Chemicals Co. Ltd. | Silberfärbungstechnik und Kit dafür |
| EP0674177A1 (de) * | 1992-12-10 | 1995-09-27 | NOVIK, Anatoly Matveevich | Verfahren zur diagnose von malignen tumoren in menschen |
| EP1368632A2 (de) * | 2001-03-08 | 2003-12-10 | Pierce Biotechnolgoy, Inc. | Direkte nachweis von molekülen in polyacrylamide gel |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6074818A (en) * | 1990-08-24 | 2000-06-13 | The University Of Tennessee Research Corporation | Fingerprinting of nucleic acids, products and methods |
| WO1992017611A1 (en) * | 1991-03-28 | 1992-10-15 | The University Of Tennessee Research Corporation | Dna silver staining |
| US5595878A (en) * | 1995-06-02 | 1997-01-21 | Boron Biologicals, Inc. | Detection of biopolymers and biooligomers with boron hydride labels |
| US6635489B2 (en) * | 2000-11-20 | 2003-10-21 | Invitrogen Corporation | Materials for enhancing staining of biopolymers in matrices |
| US7498140B2 (en) * | 2001-03-08 | 2009-03-03 | Pierce Biotechnology, Inc. | Direct detection of biomolecules in a gel |
| CA2442282A1 (en) * | 2001-03-29 | 2002-10-10 | Cellect Technologies Corp. | Methods devices and systems for sorting and separating particles |
| CN104458388A (zh) * | 2014-12-01 | 2015-03-25 | 中国农业科学院棉花研究所 | 一种page的高效银染方法 |
| CN111841496B (zh) * | 2020-07-24 | 2022-02-22 | 泉州南京大学环保产业研究院 | 一种快速制备载银活性炭的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3997656A (en) * | 1973-06-25 | 1976-12-14 | Applied Bioscience | Tissue staining method and composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4416998A (en) * | 1981-04-02 | 1983-11-22 | The Upjohn Company | Silver stain procedure and kit for same |
| US4468466A (en) * | 1982-02-16 | 1984-08-28 | The United States Of America As Represented By The Department Of Health And Human Services | Silver stains for protein in gels--a modified procedure |
-
1983
- 1983-05-26 JP JP58091487A patent/JPS59217164A/ja active Granted
-
1984
- 1984-05-18 US US06/611,984 patent/US4582808A/en not_active Expired - Lifetime
- 1984-05-22 EP EP84105847A patent/EP0127841B1/de not_active Expired
- 1984-05-22 AT AT84105847T patent/ATE36195T1/de active
- 1984-05-22 DE DE8484105847T patent/DE3473177D1/de not_active Expired
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3997656A (en) * | 1973-06-25 | 1976-12-14 | Applied Bioscience | Tissue staining method and composition |
Non-Patent Citations (1)
| Title |
|---|
| ELECTROPHORESIS, vol. 2, no. 1, February 1981 D.W. SAMMONS, L.D. ADAMS, E.E. NISHIZAWA "Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels" pages 135-141 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0359281A2 (de) * | 1988-09-16 | 1990-03-21 | Daiichi Pure Chemicals Co. Ltd. | Silberfärbungstechnik und Kit dafür |
| EP0359281A3 (de) * | 1988-09-16 | 1991-01-16 | Daiichi Pure Chemicals Co. Ltd. | Silberfärbungstechnik und Kit dafür |
| EP0674177A1 (de) * | 1992-12-10 | 1995-09-27 | NOVIK, Anatoly Matveevich | Verfahren zur diagnose von malignen tumoren in menschen |
| EP0674177A4 (de) * | 1992-12-10 | 1997-12-10 | Novik Anatoly Matveevich | Verfahren zur diagnose von malignen tumoren in menschen. |
| EP1368632A2 (de) * | 2001-03-08 | 2003-12-10 | Pierce Biotechnolgoy, Inc. | Direkte nachweis von molekülen in polyacrylamide gel |
| EP1368632A4 (de) * | 2001-03-08 | 2009-04-01 | Pierce Biotechnolgoy Inc | Direkte nachweis von molekülen in polyacrylamide gel |
Also Published As
| Publication number | Publication date |
|---|---|
| US4582808A (en) | 1986-04-15 |
| ATE36195T1 (de) | 1988-08-15 |
| JPS59217164A (ja) | 1984-12-07 |
| EP0127841B1 (de) | 1988-08-03 |
| JPH0129423B2 (de) | 1989-06-09 |
| DE3473177D1 (en) | 1988-09-08 |
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