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DE602004009022D1 - Methode und Reagentien zur Extraktion von RNA und Methode zur Analyse von biologischem Material - Google Patents

Methode und Reagentien zur Extraktion von RNA und Methode zur Analyse von biologischem Material

Info

Publication number
DE602004009022D1
DE602004009022D1 DE602004009022T DE602004009022T DE602004009022D1 DE 602004009022 D1 DE602004009022 D1 DE 602004009022D1 DE 602004009022 T DE602004009022 T DE 602004009022T DE 602004009022 T DE602004009022 T DE 602004009022T DE 602004009022 D1 DE602004009022 D1 DE 602004009022D1
Authority
DE
Germany
Prior art keywords
rna
reagents
extraction
analysis
biological material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004009022T
Other languages
English (en)
Other versions
DE602004009022T2 (de
Inventor
Yoshihiro Yamashita
Toshinari Sakurai
Norihito Kuno
Kenko Uchida
Toshiaki Yokobayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi High Tech Corp
Original Assignee
Hitachi High Technologies Corp
Hitachi High Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi High Technologies Corp, Hitachi High Tech Corp filed Critical Hitachi High Technologies Corp
Publication of DE602004009022D1 publication Critical patent/DE602004009022D1/de
Application granted granted Critical
Publication of DE602004009022T2 publication Critical patent/DE602004009022T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE602004009022T 2003-11-07 2004-11-05 Verfahren und Reagentien zur Extraktion von RNA und Verfahren zur Analyse von biologischem Material Expired - Lifetime DE602004009022T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003378516 2003-11-07
JP2003378516A JP3714940B2 (ja) 2003-11-07 2003-11-07 Ｒｎａ抽出方法、および生体材料の分析方法

Publications (2)

Publication Number Publication Date
DE602004009022D1 true DE602004009022D1 (de) 2007-10-31
DE602004009022T2 DE602004009022T2 (de) 2008-01-10

Family

ID=34431348

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004009022T Expired - Lifetime DE602004009022T2 (de) 2003-11-07 2004-11-05 Verfahren und Reagentien zur Extraktion von RNA und Verfahren zur Analyse von biologischem Material

Country Status (5)

Country Link
US (2) US20050123965A1 (de)
EP (1) EP1529841B1 (de)
JP (1) JP3714940B2 (de)
CN (1) CN100537590C (de)
DE (1) DE602004009022T2 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4699868B2 (ja) 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
WO2008002882A2 (en) 2006-06-26 2008-01-03 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US8163535B2 (en) 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
ES2394815T3 (es) * 2006-12-13 2013-02-05 F. Hoffmann-La Roche Ag Utilización de TDE para el aislamiento de ácidos nucleicos
ATE490320T1 (de) * 2006-12-13 2010-12-15 Hoffmann La Roche Verwendung von acetalen zur isolation von nukleinsäuren
WO2008135196A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
US20110060135A1 (en) * 2007-11-29 2011-03-10 New England Biolabs, Inc. Selective Purification of Small RNAs from Mixtures
CA2705267C (en) * 2007-11-30 2018-07-31 Ge Healthcare Bio-Sciences Corp. Method for isolation of genomic dna, rna and proteins from a single sample
JP5367078B2 (ja) 2008-08-01 2013-12-11 エフ．ホフマン−ラ ロシュ アーゲー ｍＲＮＡ定量のための改善された溶解および逆転写
DE102008045705A1 (de) * 2008-09-04 2010-04-22 Macherey, Nagel Gmbh & Co. Kg Handelsgesellschaft Verfahren zur Gewinnung von kurzer RNA sowie Kit hierfür
US20110172405A1 (en) * 2008-09-17 2011-07-14 Ge Healthcare Bio-Sciences Corp. Method for small rna isolation
CN101864414A (zh) * 2010-07-12 2010-10-20 大连海洋大学 仿刺参体壁总rna提取方法
CN102140451A (zh) * 2010-12-09 2011-08-03 中国农业大学 一种提取dna和rna的方法
US9051563B2 (en) 2011-01-14 2015-06-09 Zymo Research Corporation Nucleic acid purification
US8629264B2 (en) 2011-05-19 2014-01-14 Blood Cell Storage, Inc. Gravity flow fluidic device for nucleic acid extraction
US9518901B2 (en) 2011-06-29 2016-12-13 Kabushiki Kaisha Dnaform Pretreatment method of biological sample, detection method of RNA, and pretreatment kit
DE102011080853B4 (de) * 2011-08-11 2014-03-27 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von RNA aus Volblutproben
CN102586230A (zh) * 2012-03-02 2012-07-18 山东农业大学 基于pcr的玉米半粒种子dna快速提取方法
US9040675B2 (en) * 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9040679B2 (en) * 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9206469B2 (en) 2012-07-18 2015-12-08 Zymo Research Corporation Nucleic acid purification
CN102911930A (zh) * 2012-09-18 2013-02-06 湖南农业大学 一种红花檵木叶片总rna的提取方法
WO2014099121A1 (en) * 2012-12-20 2014-06-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9534214B2 (en) * 2013-10-31 2017-01-03 General Electric Company Substrates and associated methods for elution of nucleic acids
CN107429209B (zh) * 2015-01-22 2021-05-07 爱科来株式会社 目标分析芯片以及目标分析方法
FI4035762T3 (fi) 2015-09-09 2023-12-04 Drawbridge Health Inc Laitteita näytteiden keräämistä, stabilointia ja säilytystä varten
US11434480B2 (en) * 2017-09-27 2022-09-06 Industry Foundation Of Chonnam National University Viral RNA extracting composition and viral RNA extracting method
CN111254141B (zh) * 2020-04-28 2020-08-04 博奥生物集团有限公司 核酸提取组合物及其应用，含有该核酸提取组合物的试剂、试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US192667A (en) * 1877-07-03 Improvement in fastening devices for axle-box covers
US5503979A (en) * 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
EP1146049B1 (de) * 1994-02-11 2005-08-17 Qiagen GmbH Verfahren zur Trennung von Doppelstrang/Einzelstrangnukleinsäurestrukturen
JPH09327291A (ja) * 1996-06-11 1997-12-22 Toyobo Co Ltd Ｒｎａの抽出精製方法
DE19912799B4 (de) * 1998-03-12 2004-12-30 AGOWA Gesellschaft für molekularbiologische Technologie mbH Superparamagnetisches Adsorptionsmaterial und seine Verwendung
JP3602071B2 (ja) * 2001-06-05 2004-12-15 株式会社日立製作所 核酸の精製分離方法

Also Published As

Publication number Publication date
CN1637012A (zh) 2005-07-13
US20050123965A1 (en) 2005-06-09
EP1529841B1 (de) 2007-09-19
JP2005137295A (ja) 2005-06-02
JP3714940B2 (ja) 2005-11-09
EP1529841A1 (de) 2005-05-11
US20090111114A1 (en) 2009-04-30
CN100537590C (zh) 2009-09-09
DE602004009022T2 (de) 2008-01-10

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